                         SEQUENCE LISTING

<110>  VIB VZW
       UNIVERSITEIT GENT
 
<120>  Membrane span-kinase fusion protein and the uses thereof

<130>  JT/RKISS/407

<150>  EP 12158276.1
<151>  2012-03-06

<160>  37    

<170>  PatentIn version 3.5

<210>  1
<211>  1187
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Pro Leu Arg His Trp Gly Met Ala Arg Gly Ser Lys Pro Val Gly 
1               5                   10                  15      


Asp Gly Ala Gln Pro Met Ala Ala Met Gly Gly Leu Lys Val Leu Leu 
            20                  25                  30          


His Trp Ala Gly Pro Gly Gly Gly Glu Pro Trp Val Thr Phe Ser Glu 
        35                  40                  45              


Ser Ser Leu Thr Ala Glu Glu Val Cys Ile His Ile Ala His Lys Val 
    50                  55                  60                  


Gly Ile Thr Pro Pro Cys Phe Asn Leu Phe Ala Leu Phe Asp Ala Gln 
65                  70                  75                  80  


Ala Gln Val Trp Leu Pro Pro Asn His Ile Leu Glu Ile Pro Arg Asp 
                85                  90                  95      


Ala Ser Leu Met Leu Tyr Phe Arg Ile Arg Phe Tyr Phe Arg Asn Trp 
            100                 105                 110         


His Gly Met Asn Pro Arg Glu Pro Ala Val Tyr Arg Cys Gly Pro Pro 
        115                 120                 125             


Gly Thr Glu Ala Ser Ser Asp Gln Thr Ala Gln Gly Met Gln Leu Leu 
    130                 135                 140                 


Asp Pro Ala Ser Phe Glu Tyr Leu Phe Glu Gln Gly Lys His Glu Phe 
145                 150                 155                 160 


Val Asn Asp Val Ala Ser Leu Trp Glu Leu Ser Thr Glu Glu Glu Ile 
                165                 170                 175     


His His Phe Lys Asn Glu Ser Leu Gly Met Ala Phe Leu His Leu Cys 
            180                 185                 190         


His Leu Ala Leu Arg His Gly Ile Pro Leu Glu Glu Val Ala Lys Lys 
        195                 200                 205             


Thr Ser Phe Lys Asp Cys Ile Pro Arg Ser Phe Arg Arg His Ile Arg 
    210                 215                 220                 


Gln His Ser Ala Leu Thr Arg Leu Arg Leu Arg Asn Val Phe Arg Arg 
225                 230                 235                 240 


Phe Leu Arg Asp Phe Gln Pro Gly Arg Leu Ser Gln Gln Met Val Met 
                245                 250                 255     


Val Lys Tyr Leu Ala Thr Leu Glu Arg Leu Ala Pro Arg Phe Gly Thr 
            260                 265                 270         


Glu Arg Val Pro Val Cys His Leu Arg Leu Leu Ala Gln Ala Glu Gly 
        275                 280                 285             


Glu Pro Cys Tyr Ile Arg Asp Ser Gly Val Ala Pro Thr Asp Pro Gly 
    290                 295                 300                 


Pro Glu Ser Ala Ala Gly Pro Pro Thr His Glu Val Leu Val Thr Gly 
305                 310                 315                 320 


Thr Gly Gly Ile Gln Trp Trp Pro Val Glu Glu Glu Val Asn Lys Glu 
                325                 330                 335     


Glu Gly Ser Ser Gly Ser Ser Gly Arg Asn Pro Gln Ala Ser Leu Phe 
            340                 345                 350         


Gly Lys Lys Ala Lys Ala His Lys Ala Val Gly Gln Pro Ala Asp Arg 
        355                 360                 365             


Pro Arg Glu Pro Leu Trp Ala Tyr Phe Cys Asp Phe Arg Asp Ile Thr 
    370                 375                 380                 


His Val Val Leu Lys Glu His Cys Val Ser Ile His Arg Gln Asp Asn 
385                 390                 395                 400 


Lys Cys Leu Glu Leu Ser Leu Pro Ser Arg Ala Ala Ala Leu Ser Phe 
                405                 410                 415     


Val Ser Leu Val Asp Gly Tyr Phe Arg Leu Thr Ala Asp Ser Ser His 
            420                 425                 430         


Tyr Leu Cys His Glu Val Ala Pro Pro Arg Leu Val Met Ser Ile Arg 
        435                 440                 445             


Asp Gly Ile His Gly Pro Leu Leu Glu Pro Phe Val Gln Ala Lys Leu 
    450                 455                 460                 


Arg Pro Glu Asp Gly Leu Tyr Leu Ile His Trp Ser Thr Ser His Pro 
465                 470                 475                 480 


Tyr Arg Leu Ile Leu Thr Val Ala Gln Arg Ser Gln Ala Pro Asp Gly 
                485                 490                 495     


Met Gln Ser Leu Arg Leu Arg Lys Phe Pro Ile Glu Gln Gln Asp Gly 
            500                 505                 510         


Ala Phe Val Leu Glu Gly Trp Gly Arg Ser Phe Pro Ser Val Arg Glu 
        515                 520                 525             


Leu Gly Ala Ala Leu Gln Gly Cys Leu Leu Arg Ala Gly Asp Asp Cys 
    530                 535                 540                 


Phe Ser Leu Arg Arg Cys Cys Leu Pro Gln Pro Gly Glu Thr Ser Asn 
545                 550                 555                 560 


Leu Ile Ile Met Arg Gly Ala Arg Ala Ser Pro Arg Thr Leu Asn Leu 
                565                 570                 575     


Ser Gln Leu Ser Phe His Arg Val Asp Gln Lys Glu Ile Thr Gln Leu 
            580                 585                 590         


Ser His Leu Gly Gln Gly Thr Arg Thr Asn Val Tyr Glu Gly Arg Leu 
        595                 600                 605             


Arg Val Glu Gly Ser Gly Asp Pro Glu Glu Gly Lys Met Asp Asp Glu 
    610                 615                 620                 


Asp Pro Leu Val Pro Gly Arg Asp Arg Gly Gln Glu Leu Arg Val Val 
625                 630                 635                 640 


Leu Lys Val Leu Asp Pro Ser His His Asp Ile Ala Leu Ala Phe Tyr 
                645                 650                 655     


Glu Thr Ala Ser Leu Met Ser Gln Val Ser His Thr His Leu Ala Phe 
            660                 665                 670         


Val His Gly Val Cys Val Arg Gly Pro Glu Asn Ile Met Val Thr Glu 
        675                 680                 685             


Tyr Val Glu His Gly Pro Leu Asp Val Trp Leu Arg Arg Glu Arg Gly 
    690                 695                 700                 


His Val Pro Met Ala Trp Lys Met Val Val Ala Gln Gln Leu Ala Ser 
705                 710                 715                 720 


Ala Leu Ser Tyr Leu Glu Asn Lys Asn Leu Val His Gly Asn Val Cys 
                725                 730                 735     


Gly Arg Asn Ile Leu Leu Ala Arg Leu Gly Leu Ala Glu Gly Thr Ser 
            740                 745                 750         


Pro Phe Ile Lys Leu Ser Asp Pro Gly Val Gly Leu Gly Ala Leu Ser 
        755                 760                 765             


Arg Glu Glu Arg Val Glu Arg Ile Pro Trp Leu Ala Pro Glu Cys Leu 
    770                 775                 780                 


Pro Gly Gly Ala Asn Ser Leu Ser Thr Ala Met Asp Lys Trp Gly Phe 
785                 790                 795                 800 


Gly Ala Thr Leu Leu Glu Ile Cys Phe Asp Gly Glu Ala Pro Leu Gln 
                805                 810                 815     


Ser Arg Ser Pro Ser Glu Lys Glu His Phe Tyr Gln Arg Gln His Arg 
            820                 825                 830         


Leu Pro Glu Pro Ser Cys Pro Gln Leu Ala Thr Leu Thr Ser Gln Cys 
        835                 840                 845             


Leu Thr Tyr Glu Pro Thr Gln Arg Pro Ser Phe Arg Thr Ile Leu Arg 
    850                 855                 860                 


Asp Leu Thr Arg Leu Gln Pro His Asn Leu Ala Asp Val Leu Thr Val 
865                 870                 875                 880 


Asn Pro Asp Ser Pro Ala Ser Asp Pro Thr Val Phe His Lys Arg Tyr 
                885                 890                 895     


Leu Lys Lys Ile Arg Asp Leu Gly Glu Gly His Phe Gly Lys Val Ser 
            900                 905                 910         


Leu Tyr Cys Tyr Asp Pro Thr Asn Asp Gly Thr Gly Glu Met Val Ala 
        915                 920                 925             


Val Lys Ala Leu Lys Ala Asp Cys Gly Pro Gln His Arg Ser Gly Trp 
    930                 935                 940                 


Lys Gln Glu Ile Asp Ile Leu Arg Thr Leu Tyr His Glu His Ile Ile 
945                 950                 955                 960 


Lys Tyr Lys Gly Cys Cys Glu Asp Gln Gly Glu Lys Ser Leu Gln Leu 
                965                 970                 975     


Val Met Glu Tyr Val Pro Leu Gly Ser Leu Arg Asp Tyr Leu Pro Arg 
            980                 985                 990         


His Ser Ile Gly Leu Ala Gln Leu  Leu Leu Phe Ala Gln  Gln Ile Cys 
        995                 1000                 1005             


Glu Gly  Met Ala Tyr Leu His  Ala Gln His Tyr Ile  His Arg Asp 
    1010                 1015                 1020             


Leu Ala  Ala Arg Asn Val Leu  Leu Asp Asn Asp Arg  Leu Val Lys 
    1025                 1030                 1035             


Ile Gly  Asp Phe Gly Leu Ala  Lys Ala Val Pro Glu  Gly His Glu 
    1040                 1045                 1050             


Tyr Tyr  Arg Val Arg Glu Asp  Gly Asp Ser Pro Val  Phe Trp Tyr 
    1055                 1060                 1065             


Ala Pro  Glu Cys Leu Lys Glu  Tyr Lys Phe Tyr Tyr  Ala Ser Asp 
    1070                 1075                 1080             


Val Trp  Ser Phe Gly Val Thr  Leu Tyr Glu Leu Leu  Thr His Cys 
    1085                 1090                 1095             


Asp Ser  Ser Gln Ser Pro Pro  Thr Lys Phe Leu Glu  Leu Ile Gly 
    1100                 1105                 1110             


Ile Ala  Gln Gly Gln Met Thr  Val Leu Arg Leu Thr  Glu Leu Leu 
    1115                 1120                 1125             


Glu Arg  Gly Glu Arg Leu Pro  Arg Pro Asp Lys Cys  Pro Cys Glu 
    1130                 1135                 1140             


Val Tyr  His Leu Met Lys Asn  Cys Trp Glu Thr Glu  Ala Ser Phe 
    1145                 1150                 1155             


Arg Pro  Thr Phe Glu Asn Leu  Ile Pro Ile Leu Lys  Thr Val His 
    1160                 1165                 1170             


Glu Lys  Tyr Gln Gly Gln Ala  Pro Ser Val Phe Ser  Val Cys 
    1175                 1180                 1185         


<210>  2
<211>  158
<212>  PRT
<213>  Homo sapiens

<400>  2

Thr Val Val His Ser Gly Tyr Arg His Gln Val Pro Ser Val Gln Val 
1               5                   10                  15      


Phe Ser Arg Ser Glu Ser Thr Gln Pro Leu Leu Asp Ser Glu Glu Arg 
            20                  25                  30          


Pro Glu Asp Leu Gln Leu Val Asp His Val Asp Gly Gly Asp Gly Ile 
        35                  40                  45              


Leu Pro Arg Gln Gln Tyr Phe Lys Gln Asn Cys Ser Gln His Glu Ser 
    50                  55                  60                  


Ser Pro Asp Ile Ser His Phe Glu Arg Ser Lys Gln Val Ser Ser Val 
65                  70                  75                  80  


Asn Glu Glu Asp Phe Val Arg Leu Lys Gln Gln Ile Ser Asp His Ile 
                85                  90                  95      


Ser Gln Ser Cys Gly Ser Gly Gln Met Lys Met Phe Gln Glu Val Ser 
            100                 105                 110         


Ala Ala Asp Ala Phe Gly Pro Gly Thr Glu Gly Gln Val Glu Arg Phe 
        115                 120                 125             


Glu Thr Val Gly Met Glu Ala Ala Thr Asp Glu Gly Met Pro Lys Ser 
    130                 135                 140                 


Tyr Leu Pro Gln Thr Val Arg Gln Gly Gly Tyr Met Pro Gln 
145                 150                 155             


<210>  3
<211>  985
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SSTR2-Tyk2(C) fusion construct

<400>  3

Met Asp Met Ala Asp Glu Pro Leu Asn Gly Ser His Thr Trp Leu Ser 
1               5                   10                  15      


Ile Pro Phe Asp Leu Asn Gly Ser Val Val Ser Thr Asn Thr Ser Asn 
            20                  25                  30          


Gln Thr Glu Pro Tyr Tyr Asp Leu Thr Ser Asn Ala Val Leu Thr Phe 
        35                  40                  45              


Ile Tyr Phe Val Val Cys Ile Ile Gly Leu Cys Gly Asn Thr Leu Val 
    50                  55                  60                  


Ile Tyr Val Ile Leu Arg Tyr Ala Lys Met Lys Thr Ile Thr Asn Ile 
65                  70                  75                  80  


Tyr Ile Leu Asn Leu Ala Ile Ala Asp Glu Leu Phe Met Leu Gly Leu 
                85                  90                  95      


Pro Phe Leu Ala Met Gln Val Ala Leu Val His Trp Pro Phe Gly Lys 
            100                 105                 110         


Ala Ile Cys Arg Val Val Met Thr Val Asp Gly Ile Asn Gln Phe Thr 
        115                 120                 125             


Ser Ile Phe Cys Leu Thr Val Met Ser Ile Asp Arg Tyr Leu Ala Val 
    130                 135                 140                 


Val His Pro Ile Lys Ser Ala Lys Trp Arg Arg Pro Arg Thr Ala Lys 
145                 150                 155                 160 


Met Ile Thr Met Ala Val Trp Gly Val Ser Leu Leu Val Ile Leu Pro 
                165                 170                 175     


Ile Met Ile Tyr Ala Gly Leu Arg Ser Asn Gln Trp Gly Arg Ser Ser 
            180                 185                 190         


Cys Thr Ile Asn Trp Pro Gly Glu Ser Gly Ala Trp Tyr Thr Gly Phe 
        195                 200                 205             


Ile Ile Tyr Thr Phe Ile Leu Gly Phe Leu Val Pro Leu Thr Ile Ile 
    210                 215                 220                 


Cys Leu Cys Tyr Leu Phe Ile Ile Ile Lys Val Lys Ser Ser Gly Ile 
225                 230                 235                 240 


Arg Val Gly Ser Ser Lys Arg Lys Lys Ser Glu Lys Lys Val Thr Arg 
                245                 250                 255     


Met Val Ser Ile Val Val Ala Val Phe Ile Phe Cys Trp Leu Pro Phe 
            260                 265                 270         


Tyr Ile Phe Asn Val Ser Ser Val Ser Met Ala Ile Ser Pro Thr Pro 
        275                 280                 285             


Ala Leu Lys Gly Met Phe Asp Phe Val Val Val Leu Thr Tyr Ala Asn 
    290                 295                 300                 


Ser Cys Ala Asn Pro Ile Leu Tyr Ala Phe Leu Ser Asp Asn Phe Lys 
305                 310                 315                 320 


Lys Ser Phe Gln Asn Val Leu Cys Leu Val Lys Val Ser Gly Thr Asp 
                325                 330                 335     


Asp Gly Glu Arg Ser Asp Ser Lys Gln Asp Lys Ser Arg Leu Asn Glu 
            340                 345                 350         


Thr Thr Glu Thr Gln Arg Thr Leu Leu Asn Gly Asp Leu Gln Thr Ser 
        355                 360                 365             


Ile Ala Ala Ala Gly Gly Ser Ile Thr Gln Leu Ser His Leu Gly Gln 
    370                 375                 380                 


Gly Thr Arg Thr Asn Val Tyr Glu Gly Arg Leu Arg Val Glu Gly Ser 
385                 390                 395                 400 


Gly Asp Pro Glu Glu Gly Lys Met Asp Asp Glu Asp Pro Leu Val Pro 
                405                 410                 415     


Gly Arg Asp Arg Gly Gln Glu Leu Arg Val Val Leu Lys Val Leu Asp 
            420                 425                 430         


Pro Ser His His Asp Ile Ala Leu Ala Phe Tyr Glu Thr Ala Ser Leu 
        435                 440                 445             


Met Ser Gln Val Ser His Thr His Leu Ala Phe Val His Gly Val Cys 
    450                 455                 460                 


Val Arg Gly Pro Glu Asn Ser Met Val Thr Glu Tyr Val Glu His Gly 
465                 470                 475                 480 


Pro Leu Asp Val Trp Leu Arg Arg Glu Arg Gly His Val Pro Met Ala 
                485                 490                 495     


Trp Lys Met Val Val Ala Gln Gln Leu Ala Ser Ala Leu Ser Tyr Leu 
            500                 505                 510         


Glu Asn Lys Asn Leu Val His Gly Asn Val Cys Gly Arg Asn Ile Leu 
        515                 520                 525             


Leu Ala Arg Leu Gly Leu Ala Glu Gly Thr Ser Pro Phe Ile Lys Leu 
    530                 535                 540                 


Ser Asp Pro Gly Val Gly Leu Gly Ala Leu Ser Arg Glu Glu Arg Val 
545                 550                 555                 560 


Glu Arg Ile Pro Trp Leu Ala Pro Glu Cys Leu Pro Gly Gly Ala Asn 
                565                 570                 575     


Ser Leu Ser Thr Ala Met Asp Lys Trp Gly Phe Gly Ala Thr Leu Leu 
            580                 585                 590         


Glu Ile Cys Phe Asp Gly Glu Ala Pro Leu Gln Ser Arg Ser Pro Ser 
        595                 600                 605             


Glu Lys Glu His Phe Tyr Gln Arg Gln His Arg Leu Pro Glu Pro Ser 
    610                 615                 620                 


Cys Pro Gln Leu Ala Thr Leu Thr Ser Gln Cys Leu Thr Tyr Glu Pro 
625                 630                 635                 640 


Thr Gln Arg Pro Ser Phe Arg Thr Ile Leu Arg Asp Leu Thr Arg Val 
                645                 650                 655     


Gln Pro His Asn Leu Ala Asp Val Leu Thr Val Asn Arg Asp Ser Pro 
            660                 665                 670         


Ala Val Gly Pro Thr Thr Phe His Lys Arg Tyr Leu Lys Lys Ile Arg 
        675                 680                 685             


Asp Leu Gly Glu Gly His Phe Gly Lys Val Ser Leu Tyr Cys Tyr Asp 
    690                 695                 700                 


Pro Thr Asn Asp Gly Thr Gly Glu Met Val Ala Val Lys Ala Leu Lys 
705                 710                 715                 720 


Ala Asp Cys Gly Pro Gln His Arg Ser Gly Trp Lys Gln Glu Ile Asp 
                725                 730                 735     


Ile Leu Arg Thr Leu Tyr His Glu His Ile Ile Lys Tyr Lys Gly Cys 
            740                 745                 750         


Cys Glu Asp Gln Gly Glu Lys Ser Leu Gln Leu Val Met Glu Tyr Val 
        755                 760                 765             


Pro Leu Gly Ser Leu Arg Asp Tyr Leu Pro Arg His Ser Ile Gly Leu 
    770                 775                 780                 


Ala Gln Leu Leu Leu Phe Ala Gln Gln Ile Cys Glu Gly Met Ala Tyr 
785                 790                 795                 800 


Leu His Ala His Asp Tyr Ile His Arg Asp Leu Ala Ala Arg Asn Val 
                805                 810                 815     


Leu Leu Asp Asn Asp Arg Leu Val Lys Ile Gly Asp Phe Gly Leu Ala 
            820                 825                 830         


Lys Ala Val Pro Glu Gly His Glu Tyr Tyr Arg Val Arg Glu Asp Gly 
        835                 840                 845             


Asp Ser Pro Val Phe Trp Tyr Ala Pro Glu Cys Leu Lys Glu Tyr Lys 
    850                 855                 860                 


Phe Tyr Tyr Ala Ser Asp Val Trp Ser Phe Gly Val Thr Leu Tyr Glu 
865                 870                 875                 880 


Leu Leu Thr His Cys Asp Ser Ser Gln Ser Pro Pro Thr Lys Phe Leu 
                885                 890                 895     


Glu Leu Ile Gly Ile Ala Gln Gly Gln Met Thr Val Leu Arg Leu Thr 
            900                 905                 910         


Glu Leu Leu Glu Arg Gly Glu Arg Leu Pro Arg Pro Asp Lys Cys Pro 
        915                 920                 925             


Cys Glu Val Tyr His Leu Met Lys Asn Cys Trp Glu Thr Glu Ala Ser 
    930                 935                 940                 


Phe Arg Pro Thr Phe Glu Asn Leu Ile Pro Ile Leu Lys Thr Val His 
945                 950                 955                 960 


Glu Lys Tyr Gln Gly Gln Ala Pro Ser Val Phe Ser Val Cys Leu Glu 
                965                 970                 975     


Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
            980                 985 


<210>  4
<211>  975
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  AGTR1-Tyk2(C) fusion construct

<400>  4

Met Ile Leu Asn Ser Ser Thr Glu Asp Gly Ile Lys Arg Ile Gln Asp 
1               5                   10                  15      


Asp Cys Pro Lys Ala Gly Arg His Asn Tyr Ile Phe Val Met Ile Pro 
            20                  25                  30          


Thr Leu Tyr Ser Ile Ile Phe Val Val Gly Ile Phe Gly Asn Ser Leu 
        35                  40                  45              


Val Val Ile Val Ile Tyr Phe Tyr Met Lys Leu Lys Thr Val Ala Ser 
    50                  55                  60                  


Val Phe Leu Leu Asn Leu Ala Leu Ala Asp Leu Cys Phe Leu Leu Thr 
65                  70                  75                  80  


Leu Pro Leu Trp Ala Val Tyr Thr Ala Met Glu Tyr Arg Trp Pro Phe 
                85                  90                  95      


Gly Asn Tyr Leu Cys Lys Ile Ala Ser Ala Ser Val Ser Phe Asn Leu 
            100                 105                 110         


Tyr Ala Ser Val Phe Leu Leu Thr Cys Leu Ser Ile Asp Arg Tyr Leu 
        115                 120                 125             


Ala Ile Val His Pro Met Lys Ser Arg Leu Arg Arg Thr Met Leu Val 
    130                 135                 140                 


Ala Lys Val Thr Cys Ile Ile Ile Trp Leu Leu Ala Gly Leu Ala Ser 
145                 150                 155                 160 


Leu Pro Ala Ile Ile His Arg Asn Val Phe Phe Ile Glu Asn Thr Asn 
                165                 170                 175     


Ile Thr Val Cys Ala Phe His Tyr Glu Ser Gln Asn Ser Thr Leu Pro 
            180                 185                 190         


Ile Gly Leu Gly Leu Thr Lys Asn Ile Leu Gly Phe Leu Phe Pro Phe 
        195                 200                 205             


Leu Ile Ile Leu Thr Ser Tyr Thr Leu Ile Trp Lys Ala Leu Lys Lys 
    210                 215                 220                 


Ala Tyr Glu Ile Gln Lys Asn Lys Pro Arg Asn Asp Asp Ile Phe Lys 
225                 230                 235                 240 


Ile Ile Met Ala Ile Val Leu Phe Phe Phe Phe Ser Trp Ile Pro His 
                245                 250                 255     


Gln Ile Phe Thr Phe Leu Asp Val Leu Ile Gln Leu Gly Ile Ile Arg 
            260                 265                 270         


Asp Cys Arg Ile Ala Asp Ile Val Asp Thr Ala Met Pro Ile Thr Ile 
        275                 280                 285             


Cys Ile Ala Tyr Phe Asn Asn Cys Leu Asn Pro Leu Phe Tyr Gly Phe 
    290                 295                 300                 


Leu Gly Lys Lys Phe Lys Arg Tyr Phe Leu Gln Leu Leu Lys Tyr Ile 
305                 310                 315                 320 


Pro Pro Lys Ala Lys Ser His Ser Asn Leu Ser Thr Lys Met Ser Thr 
                325                 330                 335     


Leu Ser Tyr Arg Pro Ser Asp Asn Val Ser Ser Ser Thr Lys Lys Pro 
            340                 345                 350         


Ala Pro Cys Phe Glu Val Glu Ala Ala Ala Gly Gly Ser Ile Thr Gln 
        355                 360                 365             


Leu Ser His Leu Gly Gln Gly Thr Arg Thr Asn Val Tyr Glu Gly Arg 
    370                 375                 380                 


Leu Arg Val Glu Gly Ser Gly Asp Pro Glu Glu Gly Lys Met Asp Asp 
385                 390                 395                 400 


Glu Asp Pro Leu Val Pro Gly Arg Asp Arg Gly Gln Glu Leu Arg Val 
                405                 410                 415     


Val Leu Lys Val Leu Asp Pro Ser His His Asp Ile Ala Leu Ala Phe 
            420                 425                 430         


Tyr Glu Thr Ala Ser Leu Met Ser Gln Val Ser His Thr His Leu Ala 
        435                 440                 445             


Phe Val His Gly Val Cys Val Arg Gly Pro Glu Asn Ser Met Val Thr 
    450                 455                 460                 


Glu Tyr Val Glu His Gly Pro Leu Asp Val Trp Leu Arg Arg Glu Arg 
465                 470                 475                 480 


Gly His Val Pro Met Ala Trp Lys Met Val Val Ala Gln Gln Leu Ala 
                485                 490                 495     


Ser Ala Leu Ser Tyr Leu Glu Asn Lys Asn Leu Val His Gly Asn Val 
            500                 505                 510         


Cys Gly Arg Asn Ile Leu Leu Ala Arg Leu Gly Leu Ala Glu Gly Thr 
        515                 520                 525             


Ser Pro Phe Ile Lys Leu Ser Asp Pro Gly Val Gly Leu Gly Ala Leu 
    530                 535                 540                 


Ser Arg Glu Glu Arg Val Glu Arg Ile Pro Trp Leu Ala Pro Glu Cys 
545                 550                 555                 560 


Leu Pro Gly Gly Ala Asn Ser Leu Ser Thr Ala Met Asp Lys Trp Gly 
                565                 570                 575     


Phe Gly Ala Thr Leu Leu Glu Ile Cys Phe Asp Gly Glu Ala Pro Leu 
            580                 585                 590         


Gln Ser Arg Ser Pro Ser Glu Lys Glu His Phe Tyr Gln Arg Gln His 
        595                 600                 605             


Arg Leu Pro Glu Pro Ser Cys Pro Gln Leu Ala Thr Leu Thr Ser Gln 
    610                 615                 620                 


Cys Leu Thr Tyr Glu Pro Thr Gln Arg Pro Ser Phe Arg Thr Ile Leu 
625                 630                 635                 640 


Arg Asp Leu Thr Arg Val Gln Pro His Asn Leu Ala Asp Val Leu Thr 
                645                 650                 655     


Val Asn Arg Asp Ser Pro Ala Val Gly Pro Thr Thr Phe His Lys Arg 
            660                 665                 670         


Tyr Leu Lys Lys Ile Arg Asp Leu Gly Glu Gly His Phe Gly Lys Val 
        675                 680                 685             


Ser Leu Tyr Cys Tyr Asp Pro Thr Asn Asp Gly Thr Gly Glu Met Val 
    690                 695                 700                 


Ala Val Lys Ala Leu Lys Ala Asp Cys Gly Pro Gln His Arg Ser Gly 
705                 710                 715                 720 


Trp Lys Gln Glu Ile Asp Ile Leu Arg Thr Leu Tyr His Glu His Ile 
                725                 730                 735     


Ile Lys Tyr Lys Gly Cys Cys Glu Asp Gln Gly Glu Lys Ser Leu Gln 
            740                 745                 750         


Leu Val Met Glu Tyr Val Pro Leu Gly Ser Leu Arg Asp Tyr Leu Pro 
        755                 760                 765             


Arg His Ser Ile Gly Leu Ala Gln Leu Leu Leu Phe Ala Gln Gln Ile 
    770                 775                 780                 


Cys Glu Gly Met Ala Tyr Leu His Ala His Asp Tyr Ile His Arg Asp 
785                 790                 795                 800 


Leu Ala Ala Arg Asn Val Leu Leu Asp Asn Asp Arg Leu Val Lys Ile 
                805                 810                 815     


Gly Asp Phe Gly Leu Ala Lys Ala Val Pro Glu Gly His Glu Tyr Tyr 
            820                 825                 830         


Arg Val Arg Glu Asp Gly Asp Ser Pro Val Phe Trp Tyr Ala Pro Glu 
        835                 840                 845             


Cys Leu Lys Glu Tyr Lys Phe Tyr Tyr Ala Ser Asp Val Trp Ser Phe 
    850                 855                 860                 


Gly Val Thr Leu Tyr Glu Leu Leu Thr His Cys Asp Ser Ser Gln Ser 
865                 870                 875                 880 


Pro Pro Thr Lys Phe Leu Glu Leu Ile Gly Ile Ala Gln Gly Gln Met 
                885                 890                 895     


Thr Val Leu Arg Leu Thr Glu Leu Leu Glu Arg Gly Glu Arg Leu Pro 
            900                 905                 910         


Arg Pro Asp Lys Cys Pro Cys Glu Val Tyr His Leu Met Lys Asn Cys 
        915                 920                 925             


Trp Glu Thr Glu Ala Ser Phe Arg Pro Thr Phe Glu Asn Leu Ile Pro 
    930                 935                 940                 


Ile Leu Lys Thr Val His Glu Lys Tyr Gln Gly Gln Ala Pro Ser Val 
945                 950                 955                 960 


Phe Ser Val Cys Leu Glu Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
                965                 970                 975 


<210>  5
<211>  1593
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ERN1-Tyk2(C) fusion construct

<400>  5

Met Pro Ala Arg Arg Leu Leu Leu Leu Leu Thr Leu Leu Leu Pro Gly 
1               5                   10                  15      


Leu Gly Ile Phe Gly Ser Thr Ser Thr Val Thr Leu Pro Glu Thr Leu 
            20                  25                  30          


Leu Phe Val Ser Thr Leu Asp Gly Ser Leu His Ala Val Ser Lys Arg 
        35                  40                  45              


Thr Gly Ser Ile Lys Trp Thr Leu Lys Glu Asp Pro Val Leu Gln Val 
    50                  55                  60                  


Pro Thr His Val Glu Glu Pro Ala Phe Leu Pro Asp Pro Asn Asp Gly 
65                  70                  75                  80  


Ser Leu Tyr Thr Leu Gly Ser Lys Asn Asn Glu Gly Leu Thr Lys Leu 
                85                  90                  95      


Pro Phe Thr Ile Pro Glu Leu Val Gln Ala Ser Pro Cys Arg Ser Ser 
            100                 105                 110         


Asp Gly Ile Leu Tyr Met Gly Lys Lys Gln Asp Ile Trp Tyr Val Ile 
        115                 120                 125             


Asp Leu Leu Thr Gly Glu Lys Gln Gln Thr Leu Ser Ser Ala Phe Ala 
    130                 135                 140                 


Asp Ser Leu Cys Pro Ser Thr Ser Leu Leu Tyr Leu Gly Arg Thr Glu 
145                 150                 155                 160 


Tyr Thr Ile Thr Met Tyr Asp Thr Lys Thr Arg Glu Leu Arg Trp Asn 
                165                 170                 175     


Ala Thr Tyr Phe Asp Tyr Ala Ala Ser Leu Pro Glu Asp Asp Val Asp 
            180                 185                 190         


Tyr Lys Met Ser His Phe Val Ser Asn Gly Asp Gly Leu Val Val Thr 
        195                 200                 205             


Val Asp Ser Glu Ser Gly Asp Val Leu Trp Ile Gln Asn Tyr Ala Ser 
    210                 215                 220                 


Pro Val Val Ala Phe Tyr Val Trp Gln Arg Glu Gly Leu Arg Lys Val 
225                 230                 235                 240 


Met His Ile Asn Val Ala Val Glu Thr Leu Arg Tyr Leu Thr Phe Met 
                245                 250                 255     


Ser Gly Glu Val Gly Arg Ile Thr Lys Trp Lys Tyr Pro Phe Pro Lys 
            260                 265                 270         


Glu Thr Glu Ala Lys Ser Lys Leu Thr Pro Thr Leu Tyr Val Gly Lys 
        275                 280                 285             


Tyr Ser Thr Ser Leu Tyr Ala Ser Pro Ser Met Val His Glu Gly Val 
    290                 295                 300                 


Ala Val Val Pro Arg Gly Ser Thr Leu Pro Leu Leu Glu Gly Pro Gln 
305                 310                 315                 320 


Thr Asp Gly Val Thr Ile Gly Asp Lys Gly Glu Cys Val Ile Thr Pro 
                325                 330                 335     


Ser Thr Asp Val Lys Phe Asp Pro Gly Leu Lys Ser Lys Asn Lys Leu 
            340                 345                 350         


Asn Tyr Leu Arg Asn Tyr Trp Leu Leu Ile Gly His His Glu Thr Pro 
        355                 360                 365             


Leu Ser Ala Ser Thr Lys Met Leu Glu Arg Phe Pro Asn Asn Leu Pro 
    370                 375                 380                 


Lys His Arg Glu Asn Val Ile Pro Ala Asp Ser Glu Lys Lys Ser Phe 
385                 390                 395                 400 


Glu Glu Val Ile Asn Leu Val Asp Gln Thr Ser Glu Asn Ala Pro Thr 
                405                 410                 415     


Thr Val Ser Arg Asp Val Glu Glu Lys Pro Ala His Ala Pro Ala Arg 
            420                 425                 430         


Pro Glu Ala Pro Val Asp Ser Met Leu Lys Asp Met Ala Thr Ile Ile 
        435                 440                 445             


Leu Ser Thr Phe Leu Leu Ile Gly Trp Val Ala Phe Ile Ile Thr Tyr 
    450                 455                 460                 


Pro Leu Ser Met His Gln Gln Gln Gln Leu Gln His Gln Gln Phe Gln 
465                 470                 475                 480 


Lys Glu Leu Glu Lys Ile Gln Leu Leu Gln Gln Gln Gln Gln Gln Leu 
                485                 490                 495     


Pro Phe His Pro Pro Gly Asp Thr Ala Gln Asp Gly Glu Leu Leu Asp 
            500                 505                 510         


Thr Ser Gly Pro Tyr Ser Glu Ser Ser Gly Thr Ser Ser Pro Ser Thr 
        515                 520                 525             


Ser Pro Arg Ala Ser Asn His Ser Leu Cys Ser Gly Ser Ser Ala Ser 
    530                 535                 540                 


Lys Ala Gly Ser Ser Pro Ser Leu Glu Gln Asp Asp Gly Asp Glu Glu 
545                 550                 555                 560 


Thr Ser Val Val Ile Val Gly Lys Ile Ser Phe Cys Pro Lys Asp Val 
                565                 570                 575     


Leu Gly His Gly Ala Glu Gly Thr Ile Val Tyr Arg Gly Met Phe Asp 
            580                 585                 590         


Asn Arg Asp Val Ala Val Lys Arg Ile Leu Pro Glu Cys Phe Ser Phe 
        595                 600                 605             


Ala Asp Arg Glu Val Gln Leu Leu Arg Glu Ser Asp Glu His Pro Asn 
    610                 615                 620                 


Val Ile Arg Tyr Phe Cys Thr Glu Lys Asp Arg Gln Phe Gln Tyr Ile 
625                 630                 635                 640 


Ala Ile Glu Leu Cys Ala Ala Thr Leu Gln Glu Tyr Val Glu Gln Lys 
                645                 650                 655     


Asp Phe Ala His Leu Gly Leu Glu Pro Ile Thr Leu Leu Gln Gln Thr 
            660                 665                 670         


Thr Ser Gly Leu Ala His Leu His Ser Leu Asn Ile Val His Arg Asp 
        675                 680                 685             


Leu Lys Pro His Asn Ile Leu Ile Ser Met Pro Asn Ala His Gly Lys 
    690                 695                 700                 


Ile Lys Ala Met Ile Ser Asp Phe Gly Leu Cys Lys Lys Leu Ala Val 
705                 710                 715                 720 


Gly Arg His Ser Phe Ser Arg Arg Ser Gly Val Pro Gly Thr Glu Gly 
                725                 730                 735     


Trp Ile Ala Pro Glu Met Leu Ser Glu Asp Cys Lys Glu Asn Pro Thr 
            740                 745                 750         


Tyr Thr Val Asp Ile Phe Ser Ala Gly Cys Val Phe Tyr Tyr Val Ile 
        755                 760                 765             


Ser Glu Gly Ser His Pro Phe Gly Lys Ser Leu Gln Arg Gln Ala Asn 
    770                 775                 780                 


Ile Leu Leu Gly Ala Cys Ser Leu Asp Cys Leu His Pro Glu Lys His 
785                 790                 795                 800 


Glu Asp Val Ile Ala Arg Glu Leu Ile Glu Lys Met Ile Ala Met Asp 
                805                 810                 815     


Pro Gln Lys Arg Pro Ser Ala Lys His Val Leu Lys His Pro Phe Phe 
            820                 825                 830         


Trp Ser Leu Glu Lys Gln Leu Gln Phe Phe Gln Asp Val Ser Asp Arg 
        835                 840                 845             


Ile Glu Lys Glu Ser Leu Asp Gly Pro Ile Val Lys Gln Leu Glu Arg 
    850                 855                 860                 


Gly Gly Arg Ala Val Val Lys Met Asp Trp Arg Glu Asn Ile Thr Val 
865                 870                 875                 880 


Pro Leu Gln Thr Asp Leu Arg Lys Phe Arg Thr Tyr Lys Gly Gly Ser 
                885                 890                 895     


Val Arg Asp Leu Leu Arg Ala Met Arg Asn Lys Lys His His Tyr Arg 
            900                 905                 910         


Glu Leu Pro Ala Glu Val Arg Glu Thr Leu Gly Ser Leu Pro Asp Asp 
        915                 920                 925             


Phe Val Cys Tyr Phe Thr Ser Arg Phe Pro His Leu Leu Ala His Thr 
    930                 935                 940                 


Tyr Arg Ala Met Glu Leu Cys Ser His Glu Arg Leu Phe Gln Pro Tyr 
945                 950                 955                 960 


Tyr Phe His Glu Pro Pro Glu Pro Gln Pro Pro Val Thr Pro Asp Ala 
                965                 970                 975     


Leu Ala Ala Ala Gly Gly Ser Ile Thr Gln Leu Ser His Leu Gly Gln 
            980                 985                 990         


Gly Thr Arg Thr Asn Val Tyr Glu  Gly Arg Leu Arg Val  Glu Gly Ser 
        995                 1000                 1005             


Gly Asp  Pro Glu Glu Gly Lys  Met Asp Asp Glu Asp  Pro Leu Val 
    1010                 1015                 1020             


Pro Gly  Arg Asp Arg Gly Gln  Glu Leu Arg Val Val  Leu Lys Val 
    1025                 1030                 1035             


Leu Asp  Pro Ser His His Asp  Ile Ala Leu Ala Phe  Tyr Glu Thr 
    1040                 1045                 1050             


Ala Ser  Leu Met Ser Gln Val  Ser His Thr His Leu  Ala Phe Val 
    1055                 1060                 1065             


His Gly  Val Cys Val Arg Gly  Pro Glu Asn Ser Met  Val Thr Glu 
    1070                 1075                 1080             


Tyr Val  Glu His Gly Pro Leu  Asp Val Trp Leu Arg  Arg Glu Arg 
    1085                 1090                 1095             


Gly His  Val Pro Met Ala Trp  Lys Met Val Val Ala  Gln Gln Leu 
    1100                 1105                 1110             


Ala Ser  Ala Leu Ser Tyr Leu  Glu Asn Lys Asn Leu  Val His Gly 
    1115                 1120                 1125             


Asn Val  Cys Gly Arg Asn Ile  Leu Leu Ala Arg Leu  Gly Leu Ala 
    1130                 1135                 1140             


Glu Gly  Thr Ser Pro Phe Ile  Lys Leu Ser Asp Pro  Gly Val Gly 
    1145                 1150                 1155             


Leu Gly  Ala Leu Ser Arg Glu  Glu Arg Val Glu Arg  Ile Pro Trp 
    1160                 1165                 1170             


Leu Ala  Pro Glu Cys Leu Pro  Gly Gly Ala Asn Ser  Leu Ser Thr 
    1175                 1180                 1185             


Ala Met  Asp Lys Trp Gly Phe  Gly Ala Thr Leu Leu  Glu Ile Cys 
    1190                 1195                 1200             


Phe Asp  Gly Glu Ala Pro Leu  Gln Ser Arg Ser Pro  Ser Glu Lys 
    1205                 1210                 1215             


Glu His  Phe Tyr Gln Arg Gln  His Arg Leu Pro Glu  Pro Ser Cys 
    1220                 1225                 1230             


Pro Gln  Leu Ala Thr Leu Thr  Ser Gln Cys Leu Thr  Tyr Glu Pro 
    1235                 1240                 1245             


Thr Gln  Arg Pro Ser Phe Arg  Thr Ile Leu Arg Asp  Leu Thr Arg 
    1250                 1255                 1260             


Val Gln  Pro His Asn Leu Ala  Asp Val Leu Thr Val  Asn Arg Asp 
    1265                 1270                 1275             


Ser Pro  Ala Val Gly Pro Thr  Thr Phe His Lys Arg  Tyr Leu Lys 
    1280                 1285                 1290             


Lys Ile  Arg Asp Leu Gly Glu  Gly His Phe Gly Lys  Val Ser Leu 
    1295                 1300                 1305             


Tyr Cys  Tyr Asp Pro Thr Asn  Asp Gly Thr Gly Glu  Met Val Ala 
    1310                 1315                 1320             


Val Lys  Ala Leu Lys Ala Asp  Cys Gly Pro Gln His  Arg Ser Gly 
    1325                 1330                 1335             


Trp Lys  Gln Glu Ile Asp Ile  Leu Arg Thr Leu Tyr  His Glu His 
    1340                 1345                 1350             


Ile Ile  Lys Tyr Lys Gly Cys  Cys Glu Asp Gln Gly  Glu Lys Ser 
    1355                 1360                 1365             


Leu Gln  Leu Val Met Glu Tyr  Val Pro Leu Gly Ser  Leu Arg Asp 
    1370                 1375                 1380             


Tyr Leu  Pro Arg His Ser Ile  Gly Leu Ala Gln Leu  Leu Leu Phe 
    1385                 1390                 1395             


Ala Gln  Gln Ile Cys Glu Gly  Met Ala Tyr Leu His  Ala His Asp 
    1400                 1405                 1410             


Tyr Ile  His Arg Asp Leu Ala  Ala Arg Asn Val Leu  Leu Asp Asn 
    1415                 1420                 1425             


Asp Arg  Leu Val Lys Ile Gly  Asp Phe Gly Leu Ala  Lys Ala Val 
    1430                 1435                 1440             


Pro Glu  Gly His Glu Tyr Tyr  Arg Val Arg Glu Asp  Gly Asp Ser 
    1445                 1450                 1455             


Pro Val  Phe Trp Tyr Ala Pro  Glu Cys Leu Lys Glu  Tyr Lys Phe 
    1460                 1465                 1470             


Tyr Tyr  Ala Ser Asp Val Trp  Ser Phe Gly Val Thr  Leu Tyr Glu 
    1475                 1480                 1485             


Leu Leu  Thr His Cys Asp Ser  Ser Gln Ser Pro Pro  Thr Lys Phe 
    1490                 1495                 1500             


Leu Glu  Leu Ile Gly Ile Ala  Gln Gly Gln Met Thr  Val Leu Arg 
    1505                 1510                 1515             


Leu Thr  Glu Leu Leu Glu Arg  Gly Glu Arg Leu Pro  Arg Pro Asp 
    1520                 1525                 1530             


Lys Cys  Pro Cys Glu Val Tyr  His Leu Met Lys Asn  Cys Trp Glu 
    1535                 1540                 1545             


Thr Glu  Ala Ser Phe Arg Pro  Thr Phe Glu Asn Leu  Ile Pro Ile 
    1550                 1555                 1560             


Leu Lys  Thr Val His Glu Lys  Tyr Gln Gly Gln Ala  Pro Ser Val 
    1565                 1570                 1575             


Phe Ser  Val Cys Leu Glu Tyr  Pro Tyr Asp Val Pro  Asp Tyr Ala 
    1580                 1585                 1590             


<210>  6
<211>  612
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  gp130-ARRB2 fusion construct

<400>  6

Met Asp Tyr Lys Asp Asp Asp Asp Lys Ile Ser Thr Val Val His Ser 
1               5                   10                  15      


Gly Tyr Arg His Gln Val Pro Ser Val Gln Val Phe Ser Arg Ser Glu 
            20                  25                  30          


Ser Thr Gln Pro Leu Leu Asp Ser Glu Glu Arg Pro Glu Asp Leu Gln 
        35                  40                  45              


Leu Val Asp His Val Asp Gly Gly Asp Gly Ile Leu Pro Arg Gln Gln 
    50                  55                  60                  


Tyr Phe Lys Gln Asn Cys Ser Gln His Glu Ser Ser Pro Asp Ile Ser 
65                  70                  75                  80  


His Phe Glu Arg Ser Lys Gln Val Ser Ser Val Asn Glu Glu Asp Phe 
                85                  90                  95      


Val Arg Leu Lys Gln Gln Ile Ser Asp His Ile Ser Gln Ser Cys Gly 
            100                 105                 110         


Ser Gly Gln Met Lys Met Phe Gln Glu Val Ser Ala Ala Asp Ala Phe 
        115                 120                 125             


Gly Pro Gly Thr Glu Gly Gln Val Glu Arg Phe Glu Thr Val Gly Met 
    130                 135                 140                 


Glu Ala Ala Thr Asp Glu Gly Met Pro Lys Ser Tyr Leu Pro Gln Thr 
145                 150                 155                 160 


Val Arg Gln Gly Gly Tyr Met Pro Gln Gly Gly Ser Glu Leu Ser Thr 
                165                 170                 175     


Ser Leu Tyr Lys Lys Ala Gly Tyr Leu Pro Gln Thr Val Arg Gln Gly 
            180                 185                 190         


Gly Tyr Met Pro Gln Gly Gly Ser Glu Phe Thr Met Gly Glu Lys Pro 
        195                 200                 205             


Gly Thr Arg Val Phe Lys Lys Ser Ser Pro Asn Cys Lys Leu Thr Val 
    210                 215                 220                 


Tyr Leu Gly Lys Arg Asp Phe Val Asp His Leu Asp Lys Val Asp Pro 
225                 230                 235                 240 


Val Asp Gly Val Val Leu Val Asp Pro Asp Tyr Leu Lys Asp Arg Lys 
                245                 250                 255     


Val Phe Val Thr Leu Thr Cys Ala Phe Arg Tyr Gly Arg Glu Asp Leu 
            260                 265                 270         


Asp Val Leu Gly Leu Ser Phe Arg Lys Asp Leu Phe Ile Ala Thr Tyr 
        275                 280                 285             


Gln Ala Phe Pro Pro Val Pro Asn Pro Pro Arg Pro Pro Thr Arg Leu 
    290                 295                 300                 


Gln Asp Arg Leu Leu Arg Lys Leu Gly Gln His Ala His Pro Phe Phe 
305                 310                 315                 320 


Phe Thr Ile Pro Gln Asn Leu Pro Cys Ser Val Thr Leu Gln Pro Gly 
                325                 330                 335     


Pro Glu Asp Thr Gly Lys Ala Cys Gly Val Asp Phe Glu Ile Arg Ala 
            340                 345                 350         


Phe Cys Ala Lys Ser Leu Glu Glu Lys Ser His Lys Arg Asn Ser Val 
        355                 360                 365             


Arg Leu Val Ile Arg Lys Val Gln Phe Ala Pro Glu Lys Pro Gly Pro 
    370                 375                 380                 


Gln Pro Ser Ala Glu Thr Thr Arg His Phe Leu Met Ser Asp Arg Ser 
385                 390                 395                 400 


Leu His Leu Glu Ala Ser Leu Asp Lys Glu Leu Tyr Tyr His Gly Glu 
                405                 410                 415     


Pro Leu Asn Val Asn Val His Val Thr Asn Asn Ser Thr Lys Thr Val 
            420                 425                 430         


Lys Lys Ile Lys Val Ser Val Arg Gln Tyr Ala Asp Ile Cys Leu Phe 
        435                 440                 445             


Ser Thr Ala Gln Tyr Lys Cys Pro Val Ala Gln Leu Glu Gln Asp Asp 
    450                 455                 460                 


Gln Val Ser Pro Ser Ser Thr Phe Cys Lys Val Tyr Thr Ile Thr Pro 
465                 470                 475                 480 


Leu Leu Ser Asp Asn Arg Glu Lys Arg Gly Leu Ala Leu Asp Gly Lys 
                485                 490                 495     


Leu Lys His Glu Asp Thr Asn Leu Ala Ser Ser Thr Ile Val Lys Glu 
            500                 505                 510         


Gly Ala Asn Lys Glu Val Leu Gly Ile Leu Val Ser Tyr Arg Val Lys 
        515                 520                 525             


Val Lys Leu Val Val Ser Arg Gly Gly Asp Val Ser Val Glu Leu Pro 
    530                 535                 540                 


Phe Val Leu Met His Pro Lys Pro His Asp His Ile Pro Leu Pro Arg 
545                 550                 555                 560 


Pro Gln Ser Ala Ala Pro Glu Thr Asp Val Pro Val Asp Thr Asn Leu 
                565                 570                 575     


Ile Glu Phe Asp Thr Asn Tyr Ala Thr Asp Asp Asp Ile Val Phe Glu 
            580                 585                 590         


Asp Phe Ala Arg Leu Arg Leu Lys Gly Met Lys Asp Asp Asp Tyr Asp 
        595                 600                 605             


Asp Gln Leu Cys 
    610         


<210>  7
<211>  1187
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ERN1-gp130 fusion construct

<400>  7

Met Asp Tyr Lys Asp Asp Asp Asp Lys Ile Ser Glu Phe Thr Met Pro 
1               5                   10                  15      


Ala Arg Arg Leu Leu Leu Leu Leu Thr Leu Leu Leu Pro Gly Leu Gly 
            20                  25                  30          


Ile Phe Gly Ser Thr Ser Thr Val Thr Leu Pro Glu Thr Leu Leu Phe 
        35                  40                  45              


Val Ser Thr Leu Asp Gly Ser Leu His Ala Val Ser Lys Arg Thr Gly 
    50                  55                  60                  


Ser Ile Lys Trp Thr Leu Lys Glu Asp Pro Val Leu Gln Val Pro Thr 
65                  70                  75                  80  


His Val Glu Glu Pro Ala Phe Leu Pro Asp Pro Asn Asp Gly Ser Leu 
                85                  90                  95      


Tyr Thr Leu Gly Ser Lys Asn Asn Glu Gly Leu Thr Lys Leu Pro Phe 
            100                 105                 110         


Thr Ile Pro Glu Leu Val Gln Ala Ser Pro Cys Arg Ser Ser Asp Gly 
        115                 120                 125             


Ile Leu Tyr Met Gly Lys Lys Gln Asp Ile Trp Tyr Val Ile Asp Leu 
    130                 135                 140                 


Leu Thr Gly Glu Lys Gln Gln Thr Leu Ser Ser Ala Phe Ala Asp Ser 
145                 150                 155                 160 


Leu Cys Pro Ser Thr Ser Leu Leu Tyr Leu Gly Arg Thr Glu Tyr Thr 
                165                 170                 175     


Ile Thr Met Tyr Asp Thr Lys Thr Arg Glu Leu Arg Trp Asn Ala Thr 
            180                 185                 190         


Tyr Phe Asp Tyr Ala Ala Ser Leu Pro Glu Asp Asp Val Asp Tyr Lys 
        195                 200                 205             


Met Ser His Phe Val Ser Asn Gly Asp Gly Leu Val Val Thr Val Asp 
    210                 215                 220                 


Ser Glu Ser Gly Asp Val Leu Trp Ile Gln Asn Tyr Ala Ser Pro Val 
225                 230                 235                 240 


Val Ala Phe Tyr Val Trp Gln Arg Glu Gly Leu Arg Lys Val Met His 
                245                 250                 255     


Ile Asn Val Ala Val Glu Thr Leu Arg Tyr Leu Thr Phe Met Ser Gly 
            260                 265                 270         


Glu Val Gly Arg Ile Thr Lys Trp Lys Tyr Pro Phe Pro Lys Glu Thr 
        275                 280                 285             


Glu Ala Lys Ser Lys Leu Thr Pro Thr Leu Tyr Val Gly Lys Tyr Ser 
    290                 295                 300                 


Thr Ser Leu Tyr Ala Ser Pro Ser Met Val His Glu Gly Val Ala Val 
305                 310                 315                 320 


Val Pro Arg Gly Ser Thr Leu Pro Leu Leu Glu Gly Pro Gln Thr Asp 
                325                 330                 335     


Gly Val Thr Ile Gly Asp Lys Gly Glu Cys Val Ile Thr Pro Ser Thr 
            340                 345                 350         


Asp Val Lys Phe Asp Pro Gly Leu Lys Ser Lys Asn Lys Leu Asn Tyr 
        355                 360                 365             


Leu Arg Asn Tyr Trp Leu Leu Ile Gly His His Glu Thr Pro Leu Ser 
    370                 375                 380                 


Ala Ser Thr Lys Met Leu Glu Arg Phe Pro Asn Asn Leu Pro Lys His 
385                 390                 395                 400 


Arg Glu Asn Val Ile Pro Ala Asp Ser Glu Lys Lys Ser Phe Glu Glu 
                405                 410                 415     


Val Ile Asn Leu Val Asp Gln Thr Ser Glu Asn Ala Pro Thr Thr Val 
            420                 425                 430         


Ser Arg Asp Val Glu Glu Lys Pro Ala His Ala Pro Ala Arg Pro Glu 
        435                 440                 445             


Ala Pro Val Asp Ser Met Leu Lys Asp Met Ala Thr Ile Ile Leu Ser 
    450                 455                 460                 


Thr Phe Leu Leu Ile Gly Trp Val Ala Phe Ile Ile Thr Tyr Pro Leu 
465                 470                 475                 480 


Ser Met His Gln Gln Gln Gln Leu Gln His Gln Gln Phe Gln Lys Glu 
                485                 490                 495     


Leu Glu Lys Ile Gln Leu Leu Gln Gln Gln Gln Gln Gln Leu Pro Phe 
            500                 505                 510         


His Pro Pro Gly Asp Thr Ala Gln Asp Gly Glu Leu Leu Asp Thr Ser 
        515                 520                 525             


Gly Pro Tyr Ser Glu Ser Ser Gly Thr Ser Ser Pro Ser Thr Ser Pro 
    530                 535                 540                 


Arg Ala Ser Asn His Ser Leu Cys Ser Gly Ser Ser Ala Ser Lys Ala 
545                 550                 555                 560 


Gly Ser Ser Pro Ser Leu Glu Gln Asp Asp Gly Asp Glu Glu Thr Ser 
                565                 570                 575     


Val Val Ile Val Gly Lys Ile Ser Phe Cys Pro Lys Asp Val Leu Gly 
            580                 585                 590         


His Gly Ala Glu Gly Thr Ile Val Tyr Arg Gly Met Phe Asp Asn Arg 
        595                 600                 605             


Asp Val Ala Val Lys Arg Ile Leu Pro Glu Cys Phe Ser Phe Ala Asp 
    610                 615                 620                 


Arg Glu Val Gln Leu Leu Arg Glu Ser Asp Glu His Pro Asn Val Ile 
625                 630                 635                 640 


Arg Tyr Phe Cys Thr Glu Lys Asp Arg Gln Phe Gln Tyr Ile Ala Ile 
                645                 650                 655     


Glu Leu Cys Ala Ala Thr Leu Gln Glu Tyr Val Glu Gln Lys Asp Phe 
            660                 665                 670         


Ala His Leu Gly Leu Glu Pro Ile Thr Leu Leu Gln Gln Thr Thr Ser 
        675                 680                 685             


Gly Leu Ala His Leu His Ser Leu Asn Ile Val His Arg Asp Leu Lys 
    690                 695                 700                 


Pro His Asn Ile Leu Ile Ser Met Pro Asn Ala His Gly Lys Ile Lys 
705                 710                 715                 720 


Ala Met Ile Ser Asp Phe Gly Leu Cys Lys Lys Leu Ala Val Gly Arg 
                725                 730                 735     


His Ser Phe Ser Arg Arg Ser Gly Val Pro Gly Thr Glu Gly Trp Ile 
            740                 745                 750         


Ala Pro Glu Met Leu Ser Glu Asp Cys Lys Glu Asn Pro Thr Tyr Thr 
        755                 760                 765             


Val Asp Ile Phe Ser Ala Gly Cys Val Phe Tyr Tyr Val Ile Ser Glu 
    770                 775                 780                 


Gly Ser His Pro Phe Gly Lys Ser Leu Gln Arg Gln Ala Asn Ile Leu 
785                 790                 795                 800 


Leu Gly Ala Cys Ser Leu Asp Cys Leu His Pro Glu Lys His Glu Asp 
                805                 810                 815     


Val Ile Ala Arg Glu Leu Ile Glu Lys Met Ile Ala Met Asp Pro Gln 
            820                 825                 830         


Lys Arg Pro Ser Ala Lys His Val Leu Lys His Pro Phe Phe Trp Ser 
        835                 840                 845             


Leu Glu Lys Gln Leu Gln Phe Phe Gln Asp Val Ser Asp Arg Ile Glu 
    850                 855                 860                 


Lys Glu Ser Leu Asp Gly Pro Ile Val Lys Gln Leu Glu Arg Gly Gly 
865                 870                 875                 880 


Arg Ala Val Val Lys Met Asp Trp Arg Glu Asn Ile Thr Val Pro Leu 
                885                 890                 895     


Gln Thr Asp Leu Arg Lys Phe Arg Thr Tyr Lys Gly Gly Ser Val Arg 
            900                 905                 910         


Asp Leu Leu Arg Ala Met Arg Asn Lys Lys His His Tyr Arg Glu Leu 
        915                 920                 925             


Pro Ala Glu Val Arg Glu Thr Leu Gly Ser Leu Pro Asp Asp Phe Val 
    930                 935                 940                 


Cys Tyr Phe Thr Ser Arg Phe Pro His Leu Leu Ala His Thr Tyr Arg 
945                 950                 955                 960 


Ala Met Glu Leu Cys Ser His Glu Arg Leu Phe Gln Pro Tyr Tyr Phe 
                965                 970                 975     


His Glu Pro Pro Glu Pro Gln Pro Pro Val Thr Pro Asp Ala Leu Ser 
            980                 985                 990         


Arg Gly Ser Gly Gly Ser Gly Gly  Ser Thr Val Val His  Ser Gly Tyr 
        995                 1000                 1005             


Arg His  Gln Val Pro Ser Val  Gln Val Phe Ser Arg  Ser Glu Ser 
    1010                 1015                 1020             


Thr Gln  Pro Leu Leu Asp Ser  Glu Glu Arg Pro Glu  Asp Leu Gln 
    1025                 1030                 1035             


Leu Val  Asp His Val Asp Gly  Gly Asp Gly Ile Leu  Pro Arg Gln 
    1040                 1045                 1050             


Gln Tyr  Phe Lys Gln Asn Cys  Ser Gln His Glu Ser  Ser Pro Asp 
    1055                 1060                 1065             


Ile Ser  His Phe Glu Arg Ser  Lys Gln Val Ser Ser  Val Asn Glu 
    1070                 1075                 1080             


Glu Asp  Phe Val Arg Leu Lys  Gln Gln Ile Ser Asp  His Ile Ser 
    1085                 1090                 1095             


Gln Ser  Cys Gly Ser Gly Gln  Met Lys Met Phe Gln  Glu Val Ser 
    1100                 1105                 1110             


Ala Ala  Asp Ala Phe Gly Pro  Gly Thr Glu Gly Gln  Val Glu Arg 
    1115                 1120                 1125             


Phe Glu  Thr Val Gly Met Glu  Ala Ala Thr Asp Glu  Gly Met Pro 
    1130                 1135                 1140             


Lys Ser  Tyr Leu Pro Gln Thr  Val Arg Gln Gly Gly  Tyr Met Pro 
    1145                 1150                 1155             


Gln Gly  Gly Ser Glu Leu Ser  Thr Ser Leu Tyr Lys  Lys Ala Gly 
    1160                 1165                 1170             


Tyr Leu  Pro Gln Thr Val Arg  Gln Gly Gly Tyr Met  Pro Gln 
    1175                 1180                 1185         


<210>  8
<211>  522
<212>  PRT
<213>  Homo sapiens

<400>  8

Gly Ser Asn Lys Ser Lys Pro Lys Asp Ala Ser Gln Arg Arg Arg Ser 
1               5                   10                  15      


Leu Glu Pro Ala Glu Asn Val His Gly Ala Gly Gly Gly Ala Phe Pro 
            20                  25                  30          


Ala Ser Gln Thr Pro Ser Lys Pro Ala Ser Ala Asp Gly His Arg Gly 
        35                  40                  45              


Pro Ser Ala Ala Phe Ala Pro Ala Ala Ala Glu Pro Lys Leu Phe Gly 
    50                  55                  60                  


Gly Phe Asn Ser Ser Asp Thr Val Thr Ser Pro Gln Arg Ala Gly Pro 
65                  70                  75                  80  


Leu Ala Gly Gly Val Thr Thr Phe Val Ala Leu Tyr Asp Tyr Glu Ser 
                85                  90                  95      


Arg Thr Glu Thr Asp Leu Ser Phe Lys Lys Gly Glu Arg Leu Gln Ile 
            100                 105                 110         


Val Asn Asn Thr Glu Gly Asp Trp Trp Leu Ala His Ser Leu Ser Thr 
        115                 120                 125             


Gly Gln Thr Gly Tyr Ile Pro Ser Asn Tyr Val Ala Pro Ser Asp Ser 
    130                 135                 140                 


Ile Gln Ala Glu Glu Trp Tyr Phe Gly Lys Ile Thr Arg Arg Glu Ser 
145                 150                 155                 160 


Glu Arg Leu Leu Leu Asn Ala Glu Asn Pro Arg Gly Thr Phe Leu Val 
                165                 170                 175     


Arg Glu Ser Glu Thr Thr Lys Gly Ala Tyr Cys Leu Ser Val Ser Asp 
            180                 185                 190         


Phe Asp Asn Ala Lys Gly Leu Asn Val Lys His Tyr Lys Ile Arg Lys 
        195                 200                 205             


Leu Asp Ser Gly Gly Phe Tyr Ile Thr Ser Arg Thr Gln Phe Asn Ser 
    210                 215                 220                 


Leu Gln Gln Leu Val Ala Tyr Tyr Ser Lys His Ala Asp Gly Leu Cys 
225                 230                 235                 240 


His Arg Leu Thr Thr Val Cys Pro Thr Ser Lys Pro Gln Thr Gln Gly 
                245                 250                 255     


Leu Ala Lys Asp Ala Trp Glu Ile Pro Arg Glu Ser Leu Arg Leu Glu 
            260                 265                 270         


Val Lys Leu Gly Gln Gly Cys Phe Gly Glu Val Trp Met Gly Thr Trp 
        275                 280                 285             


Asn Gly Thr Thr Arg Val Ala Ile Lys Thr Leu Lys Pro Gly Thr Met 
    290                 295                 300                 


Ser Pro Glu Ala Phe Leu Gln Glu Ala Gln Val Met Lys Lys Leu Arg 
305                 310                 315                 320 


His Glu Lys Leu Val Gln Leu Tyr Ala Val Val Ser Glu Glu Pro Ile 
                325                 330                 335     


Tyr Ile Val Thr Glu Tyr Met Ser Lys Gly Ser Leu Leu Asp Phe Leu 
            340                 345                 350         


Lys Gly Glu Thr Gly Lys Tyr Leu Arg Leu Pro Gln Leu Val Asp Met 
        355                 360                 365             


Ala Ala Gln Ile Ala Ser Gly Met Ala Tyr Val Glu Arg Met Asn Tyr 
    370                 375                 380                 


Val His Arg Asp Leu Arg Ala Ala Asn Ile Leu Val Gly Glu Asn Leu 
385                 390                 395                 400 


Val Cys Lys Val Ala Asp Phe Gly Leu Ala Arg Leu Ile Glu Asp Asn 
                405                 410                 415     


Glu Tyr Thr Ala Arg Gln Gly Ala Lys Phe Pro Ile Lys Trp Thr Ala 
            420                 425                 430         


Pro Glu Ala Ala Leu Tyr Gly Arg Phe Thr Ile Lys Ser Asp Val Trp 
        435                 440                 445             


Ser Phe Gly Ile Leu Leu Thr Glu Leu Thr Thr Lys Gly Arg Val Pro 
    450                 455                 460                 


Tyr Pro Gly Met Val Asn Arg Glu Val Leu Asp Gln Val Glu Arg Gly 
465                 470                 475                 480 


Tyr Arg Met Pro Cys Pro Pro Glu Cys Pro Glu Ser Leu His Asp Leu 
                485                 490                 495     


Met Cys Gln Cys Trp Arg Lys Glu Pro Glu Glu Arg Pro Thr Phe Glu 
            500                 505                 510         


Tyr Leu Gln Ala Phe Leu Glu Asp Tyr Phe 
        515                 520         


<210>  9
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  9
cccaccggtc cggaattgac aagtttgtac aaaaaagc                               38


<210>  10
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  10
ggggggcccc aaccactttg tacaagaaag c                                      31


<210>  11
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  11
cccgcggccg ctggcggttc gatcacccag ctgtcccact tgg                         43


<210>  12
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  12
tctagactaa gcataatctg gaacatcata tggatactcg aggcacacgc tgaacactga       60

agg                                                                     63


<210>  13
<211>  73
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  13
ccccaattga ccatgtatcc atatgatgtt ccagattatg ctttaattaa aatcacccag       60

ctgtcccact tgg                                                          73


<210>  14
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  14
gggtctagag cggccgcacc ggtcttaatt aagtcgacga attcgcacac gctgaacact       60

gaag                                                                    64


<210>  15
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  15
cccaagcttg aattcaccat gggggagaaa cccgggac                               38


<210>  16
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  16
ggggcggccg cctagcagag ttgatcatca tag                                    33


<210>  17
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  17
cccaagcttg gtaccaccat gccggcccgg cggctgctg                              39


<210>  18
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  18
cccgcggccg cgctagcgag ggcgtctgga gtcactgg                               38


<210>  19
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  19
cccgcggccg ctggcggttc gatcacccag ctgtcccact tgg                         43


<210>  20
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  20
gggcccctaa gcataatctg gaacatcata tggatactcg aggcacacgc tgaacactga       60

agg                                                                     63


<210>  21
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  21
cccgaattca tgccggcccg gcggctgctg                                        30


<210>  22
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  22
cccctcgagg ggagggcgtc tggagtcact gg                                     32


<210>  23
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  23
cccgaattct tctgtcccaa ggatgtcctg                                        30


<210>  24
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  24
gggtaaaaag cagcccatct ggtatgttat tgacc                                  35


<210>  25
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  25
ggtcaataac ataccagatg ggctgctttt taccc                                  35


<210>  26
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  26
cccgatatct atggagacga cgcccttgaa                                        30


<210>  27
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  27
ggggcggccg cttacacagc attcaagcgg a                                      31


<210>  28
<211>  1177
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HA-Tyk2(C)-RTp66 construct

<400>  28

Met Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Leu Ile Lys Ile Thr Gln 
1               5                   10                  15      


Leu Ser His Leu Gly Gln Gly Thr Arg Thr Asn Val Tyr Glu Gly Arg 
            20                  25                  30          


Leu Arg Val Glu Gly Ser Gly Asp Pro Glu Glu Gly Lys Met Asp Asp 
        35                  40                  45              


Glu Asp Pro Leu Val Pro Gly Arg Asp Arg Gly Gln Glu Leu Arg Val 
    50                  55                  60                  


Val Leu Lys Val Leu Asp Pro Ser His His Asp Ile Ala Leu Ala Phe 
65                  70                  75                  80  


Tyr Glu Thr Ala Ser Leu Met Ser Gln Val Ser His Thr His Leu Ala 
                85                  90                  95      


Phe Val His Gly Val Cys Val Arg Gly Pro Glu Asn Ser Met Val Thr 
            100                 105                 110         


Glu Tyr Val Glu His Gly Pro Leu Asp Val Trp Leu Arg Arg Glu Arg 
        115                 120                 125             


Gly His Val Pro Met Ala Trp Lys Met Val Val Ala Gln Gln Leu Ala 
    130                 135                 140                 


Ser Ala Leu Ser Tyr Leu Glu Asn Lys Asn Leu Val His Gly Asn Val 
145                 150                 155                 160 


Cys Gly Arg Asn Ile Leu Leu Ala Arg Leu Gly Leu Ala Glu Gly Thr 
                165                 170                 175     


Ser Pro Phe Ile Lys Leu Ser Asp Pro Gly Val Gly Leu Gly Ala Leu 
            180                 185                 190         


Ser Arg Glu Glu Arg Val Glu Arg Ile Pro Trp Leu Ala Pro Glu Cys 
        195                 200                 205             


Leu Pro Gly Gly Ala Asn Ser Leu Ser Thr Ala Met Asp Lys Trp Gly 
    210                 215                 220                 


Phe Gly Ala Thr Leu Leu Glu Ile Cys Phe Asp Gly Glu Ala Pro Leu 
225                 230                 235                 240 


Gln Ser Arg Ser Pro Ser Glu Lys Glu His Phe Tyr Gln Arg Gln His 
                245                 250                 255     


Arg Leu Pro Glu Pro Ser Cys Pro Gln Leu Ala Thr Leu Thr Ser Gln 
            260                 265                 270         


Cys Leu Thr Tyr Glu Pro Thr Gln Arg Pro Ser Phe Arg Thr Ile Leu 
        275                 280                 285             


Arg Asp Leu Thr Arg Val Gln Pro His Asn Leu Ala Asp Val Leu Thr 
    290                 295                 300                 


Val Asn Arg Asp Ser Pro Ala Val Gly Pro Thr Thr Phe His Lys Arg 
305                 310                 315                 320 


Tyr Leu Lys Lys Ile Arg Asp Leu Gly Glu Gly His Phe Gly Lys Val 
                325                 330                 335     


Ser Leu Tyr Cys Tyr Asp Pro Thr Asn Asp Gly Thr Gly Glu Met Val 
            340                 345                 350         


Ala Val Lys Ala Leu Lys Ala Asp Cys Gly Pro Gln His Arg Ser Gly 
        355                 360                 365             


Trp Lys Gln Glu Ile Asp Ile Leu Arg Thr Leu Tyr His Glu His Ile 
    370                 375                 380                 


Ile Lys Tyr Lys Gly Cys Cys Glu Asp Gln Gly Glu Lys Ser Leu Gln 
385                 390                 395                 400 


Leu Val Met Glu Tyr Val Pro Leu Gly Ser Leu Arg Asp Tyr Leu Pro 
                405                 410                 415     


Arg His Ser Ile Gly Leu Ala Gln Leu Leu Leu Phe Ala Gln Gln Ile 
            420                 425                 430         


Cys Glu Gly Met Ala Tyr Leu His Ala His Asp Tyr Ile His Arg Asp 
        435                 440                 445             


Leu Ala Ala Arg Asn Val Leu Leu Asp Asn Asp Arg Leu Val Lys Ile 
    450                 455                 460                 


Gly Asp Phe Gly Leu Ala Lys Ala Val Pro Glu Gly His Glu Tyr Tyr 
465                 470                 475                 480 


Arg Val Arg Glu Asp Gly Asp Ser Pro Val Phe Trp Tyr Ala Pro Glu 
                485                 490                 495     


Cys Leu Lys Glu Tyr Lys Phe Tyr Tyr Ala Ser Asp Val Trp Ser Phe 
            500                 505                 510         


Gly Val Thr Leu Tyr Glu Leu Leu Thr His Cys Asp Ser Ser Gln Ser 
        515                 520                 525             


Pro Pro Thr Lys Phe Leu Glu Leu Ile Gly Ile Ala Gln Gly Gln Met 
    530                 535                 540                 


Thr Val Leu Arg Leu Thr Glu Leu Leu Glu Arg Gly Glu Arg Leu Pro 
545                 550                 555                 560 


Arg Pro Asp Lys Cys Pro Cys Glu Val Tyr His Leu Met Lys Asn Cys 
                565                 570                 575     


Trp Glu Thr Glu Ala Ser Phe Arg Pro Thr Phe Glu Asn Leu Ile Pro 
            580                 585                 590         


Ile Leu Lys Thr Val His Glu Lys Tyr Gln Gly Gln Ala Pro Ser Val 
        595                 600                 605             


Phe Ser Val Cys Glu Phe Gly Ser Ser Pro Ile Ser Pro Ile Glu Thr 
    610                 615                 620                 


Val Pro Val Lys Leu Lys Pro Gly Met Asp Gly Pro Lys Val Lys Gln 
625                 630                 635                 640 


Trp Pro Leu Thr Glu Glu Lys Ile Lys Ala Leu Val Glu Ile Cys Thr 
                645                 650                 655     


Glu Met Glu Lys Glu Gly Lys Ile Ser Lys Ile Gly Pro Glu Asn Pro 
            660                 665                 670         


Tyr Asn Thr Pro Val Phe Ala Ile Lys Lys Lys Asp Ser Thr Lys Trp 
        675                 680                 685             


Arg Lys Leu Val Asp Phe Arg Glu Leu Asn Lys Arg Thr Gln Asp Phe 
    690                 695                 700                 


Trp Glu Val Gln Leu Gly Ile Pro His Pro Ala Gly Leu Lys Gln Lys 
705                 710                 715                 720 


Lys Ser Val Thr Val Leu Asp Val Gly Asp Ala Tyr Phe Ser Val Pro 
                725                 730                 735     


Leu Asp Lys Asp Phe Arg Lys Tyr Thr Ala Phe Thr Ile Pro Ser Ile 
            740                 745                 750         


Asn Asn Glu Thr Pro Gly Ile Arg Tyr Gln Tyr Asn Val Leu Pro Gln 
        755                 760                 765             


Gly Trp Lys Gly Ser Pro Ala Ile Phe Gln Cys Ser Met Thr Lys Ile 
    770                 775                 780                 


Leu Glu Pro Phe Arg Lys Gln Asn Pro Asp Ile Val Ile Tyr Gln Tyr 
785                 790                 795                 800 


Met Asp Asp Leu Tyr Val Gly Ser Asp Leu Glu Ile Gly Gln His Arg 
                805                 810                 815     


Thr Lys Ile Glu Glu Leu Arg Gln His Leu Leu Arg Trp Gly Phe Thr 
            820                 825                 830         


Thr Pro Asp Lys Lys His Gln Lys Glu Pro Pro Phe Leu Trp Met Gly 
        835                 840                 845             


Tyr Glu Leu His Pro Asp Lys Trp Thr Val Gln Pro Ile Val Leu Pro 
    850                 855                 860                 


Glu Lys Asp Ser Trp Thr Val Asn Asp Ile Gln Lys Leu Val Gly Lys 
865                 870                 875                 880 


Leu Asn Trp Ala Ser Gln Ile Tyr Ala Gly Ile Lys Val Arg Gln Leu 
                885                 890                 895     


Cys Lys Leu Leu Arg Gly Thr Lys Ala Leu Thr Glu Val Val Pro Leu 
            900                 905                 910         


Thr Glu Glu Ala Glu Leu Glu Leu Ala Glu Asn Arg Glu Ile Leu Lys 
        915                 920                 925             


Glu Pro Val His Gly Val Tyr Tyr Asp Pro Ser Lys Asp Leu Ile Ala 
    930                 935                 940                 


Glu Ile Gln Lys Gln Gly Gln Gly Gln Trp Thr Tyr Gln Ile Tyr Gln 
945                 950                 955                 960 


Glu Pro Phe Lys Asn Leu Lys Thr Gly Lys Tyr Ala Arg Met Lys Gly 
                965                 970                 975     


Ala His Thr Asn Asp Val Lys Gln Leu Thr Glu Ala Val Gln Lys Ile 
            980                 985                 990         


Ala Thr Glu Ser Ile Val Ile Trp  Gly Lys Thr Pro Lys  Phe Lys Leu 
        995                 1000                 1005             


Pro Ile  Gln Lys Glu Thr Trp  Glu Ala Trp Trp Thr  Glu Tyr Trp 
    1010                 1015                 1020             


Gln Ala  Thr Trp Ile Pro Glu  Trp Glu Phe Val Asn  Thr Pro Pro 
    1025                 1030                 1035             


Leu Val  Lys Leu Trp Tyr Gln  Leu Glu Lys Glu Pro  Ile Ile Gly 
    1040                 1045                 1050             


Ala Glu  Thr Phe Tyr Val Asp  Gly Ala Ala Asn Arg  Glu Thr Lys 
    1055                 1060                 1065             


Leu Gly  Lys Ala Gly Tyr Val  Thr Asp Arg Gly Arg  Gln Lys Val 
    1070                 1075                 1080             


Val Pro  Leu Thr Asp Thr Thr  Asn Gln Lys Thr Glu  Leu Gln Ala 
    1085                 1090                 1095             


Ile His  Leu Ala Leu Gln Asp  Ser Gly Leu Glu Val  Asn Ile Val 
    1100                 1105                 1110             


Thr Asp  Ser Gln Tyr Ala Leu  Gly Ile Ile Gln Ala  Gln Pro Asp 
    1115                 1120                 1125             


Lys Ser  Glu Ser Glu Leu Val  Ser Gln Ile Ile Glu  Gln Leu Ile 
    1130                 1135                 1140             


Lys Lys  Glu Lys Val Tyr Leu  Ala Trp Val Pro Ala  His Lys Gly 
    1145                 1150                 1155             


Ile Gly  Gly Asn Glu Gln Val  Asp Gly Leu Val Ser  Ala Gly Ile 
    1160                 1165                 1170             


Arg Lys  Val Leu 
    1175         


<210>  29
<211>  1245
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HA-Tyk2(C)-SERT construct

<400>  29

Met Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Leu Ile Lys Ile Thr Gln 
1               5                   10                  15      


Leu Ser His Leu Gly Gln Gly Thr Arg Thr Asn Val Tyr Glu Gly Arg 
            20                  25                  30          


Leu Arg Val Glu Gly Ser Gly Asp Pro Glu Glu Gly Lys Met Asp Asp 
        35                  40                  45              


Glu Asp Pro Leu Val Pro Gly Arg Asp Arg Gly Gln Glu Leu Arg Val 
    50                  55                  60                  


Val Leu Lys Val Leu Asp Pro Ser His His Asp Ile Ala Leu Ala Phe 
65                  70                  75                  80  


Tyr Glu Thr Ala Ser Leu Met Ser Gln Val Ser His Thr His Leu Ala 
                85                  90                  95      


Phe Val His Gly Val Cys Val Arg Gly Pro Glu Asn Ser Met Val Thr 
            100                 105                 110         


Glu Tyr Val Glu His Gly Pro Leu Asp Val Trp Leu Arg Arg Glu Arg 
        115                 120                 125             


Gly His Val Pro Met Ala Trp Lys Met Val Val Ala Gln Gln Leu Ala 
    130                 135                 140                 


Ser Ala Leu Ser Tyr Leu Glu Asn Lys Asn Leu Val His Gly Asn Val 
145                 150                 155                 160 


Cys Gly Arg Asn Ile Leu Leu Ala Arg Leu Gly Leu Ala Glu Gly Thr 
                165                 170                 175     


Ser Pro Phe Ile Lys Leu Ser Asp Pro Gly Val Gly Leu Gly Ala Leu 
            180                 185                 190         


Ser Arg Glu Glu Arg Val Glu Arg Ile Pro Trp Leu Ala Pro Glu Cys 
        195                 200                 205             


Leu Pro Gly Gly Ala Asn Ser Leu Ser Thr Ala Met Asp Lys Trp Gly 
    210                 215                 220                 


Phe Gly Ala Thr Leu Leu Glu Ile Cys Phe Asp Gly Glu Ala Pro Leu 
225                 230                 235                 240 


Gln Ser Arg Ser Pro Ser Glu Lys Glu His Phe Tyr Gln Arg Gln His 
                245                 250                 255     


Arg Leu Pro Glu Pro Ser Cys Pro Gln Leu Ala Thr Leu Thr Ser Gln 
            260                 265                 270         


Cys Leu Thr Tyr Glu Pro Thr Gln Arg Pro Ser Phe Arg Thr Ile Leu 
        275                 280                 285             


Arg Asp Leu Thr Arg Val Gln Pro His Asn Leu Ala Asp Val Leu Thr 
    290                 295                 300                 


Val Asn Arg Asp Ser Pro Ala Val Gly Pro Thr Thr Phe His Lys Arg 
305                 310                 315                 320 


Tyr Leu Lys Lys Ile Arg Asp Leu Gly Glu Gly His Phe Gly Lys Val 
                325                 330                 335     


Ser Leu Tyr Cys Tyr Asp Pro Thr Asn Asp Gly Thr Gly Glu Met Val 
            340                 345                 350         


Ala Val Lys Ala Leu Lys Ala Asp Cys Gly Pro Gln His Arg Ser Gly 
        355                 360                 365             


Trp Lys Gln Glu Ile Asp Ile Leu Arg Thr Leu Tyr His Glu His Ile 
    370                 375                 380                 


Ile Lys Tyr Lys Gly Cys Cys Glu Asp Gln Gly Glu Lys Ser Leu Gln 
385                 390                 395                 400 


Leu Val Met Glu Tyr Val Pro Leu Gly Ser Leu Arg Asp Tyr Leu Pro 
                405                 410                 415     


Arg His Ser Ile Gly Leu Ala Gln Leu Leu Leu Phe Ala Gln Gln Ile 
            420                 425                 430         


Cys Glu Gly Met Ala Tyr Leu His Ala His Asp Tyr Ile His Arg Asp 
        435                 440                 445             


Leu Ala Ala Arg Asn Val Leu Leu Asp Asn Asp Arg Leu Val Lys Ile 
    450                 455                 460                 


Gly Asp Phe Gly Leu Ala Lys Ala Val Pro Glu Gly His Glu Tyr Tyr 
465                 470                 475                 480 


Arg Val Arg Glu Asp Gly Asp Ser Pro Val Phe Trp Tyr Ala Pro Glu 
                485                 490                 495     


Cys Leu Lys Glu Tyr Lys Phe Tyr Tyr Ala Ser Asp Val Trp Ser Phe 
            500                 505                 510         


Gly Val Thr Leu Tyr Glu Leu Leu Thr His Cys Asp Ser Ser Gln Ser 
        515                 520                 525             


Pro Pro Thr Lys Phe Leu Glu Leu Ile Gly Ile Ala Gln Gly Gln Met 
    530                 535                 540                 


Thr Val Leu Arg Leu Thr Glu Leu Leu Glu Arg Gly Glu Arg Leu Pro 
545                 550                 555                 560 


Arg Pro Asp Lys Cys Pro Cys Glu Val Tyr His Leu Met Lys Asn Cys 
                565                 570                 575     


Trp Glu Thr Glu Ala Ser Phe Arg Pro Thr Phe Glu Asn Leu Ile Pro 
            580                 585                 590         


Ile Leu Lys Thr Val His Glu Lys Tyr Gln Gly Gln Ala Pro Ser Val 
        595                 600                 605             


Phe Ser Val Cys Glu Leu Ser Met Glu Thr Thr Pro Leu Asn Ser Gln 
    610                 615                 620                 


Lys Gln Leu Ser Ala Cys Glu Asp Gly Glu Asp Cys Gln Glu Asn Gly 
625                 630                 635                 640 


Val Leu Gln Lys Val Val Pro Thr Pro Gly Asp Lys Val Glu Ser Gly 
                645                 650                 655     


Gln Ile Ser Asn Gly Tyr Ser Ala Val Pro Ser Pro Gly Ala Gly Asp 
            660                 665                 670         


Asp Thr Arg His Ser Ile Pro Ala Thr Thr Thr Thr Leu Val Ala Glu 
        675                 680                 685             


Leu His Gln Gly Glu Arg Glu Thr Trp Gly Lys Lys Val Asp Phe Leu 
    690                 695                 700                 


Leu Ser Val Ile Gly Tyr Ala Val Asp Leu Gly Asn Val Trp Arg Phe 
705                 710                 715                 720 


Pro Tyr Ile Cys Tyr Gln Asn Gly Gly Gly Ala Phe Leu Leu Pro Tyr 
                725                 730                 735     


Thr Ile Met Ala Ile Phe Gly Gly Ile Pro Leu Phe Tyr Met Glu Leu 
            740                 745                 750         


Ala Leu Gly Gln Tyr His Arg Asn Gly Cys Ile Ser Ile Trp Arg Lys 
        755                 760                 765             


Ile Cys Pro Ile Phe Lys Gly Ile Gly Tyr Ala Ile Cys Ile Ile Ala 
    770                 775                 780                 


Phe Tyr Ile Ala Ser Tyr Tyr Asn Thr Ile Met Ala Trp Ala Leu Tyr 
785                 790                 795                 800 


Tyr Leu Ile Ser Ser Phe Thr Asp Gln Leu Pro Trp Thr Ser Cys Lys 
                805                 810                 815     


Asn Ser Trp Asn Thr Gly Asn Cys Thr Asn Tyr Phe Ser Glu Asp Asn 
            820                 825                 830         


Ile Thr Trp Thr Leu His Ser Thr Ser Pro Ala Glu Glu Phe Tyr Thr 
        835                 840                 845             


Arg His Val Leu Gln Ile His Arg Ser Lys Gly Leu Gln Asp Leu Gly 
    850                 855                 860                 


Gly Ile Ser Trp Gln Leu Ala Leu Cys Ile Met Leu Ile Phe Thr Val 
865                 870                 875                 880 


Ile Tyr Phe Ser Ile Trp Lys Gly Val Lys Thr Ser Gly Lys Val Val 
                885                 890                 895     


Trp Val Thr Ala Thr Phe Pro Tyr Ile Ile Leu Ser Val Leu Leu Val 
            900                 905                 910         


Arg Gly Ala Thr Leu Pro Gly Ala Trp Arg Gly Val Leu Phe Tyr Leu 
        915                 920                 925             


Lys Pro Asn Trp Gln Lys Leu Leu Glu Thr Gly Val Trp Ile Asp Ala 
    930                 935                 940                 


Ala Ala Gln Ile Phe Phe Ser Leu Gly Pro Gly Phe Gly Val Leu Leu 
945                 950                 955                 960 


Ala Phe Ala Ser Tyr Asn Lys Phe Asn Asn Asn Cys Tyr Gln Asp Ala 
                965                 970                 975     


Leu Val Thr Ser Val Val Asn Cys Met Thr Ser Phe Val Ser Gly Phe 
            980                 985                 990         


Val Ile Phe Thr Val Leu Gly Tyr  Met Ala Glu Met Arg  Asn Glu Asp 
        995                 1000                 1005             


Val Ser  Glu Val Ala Lys Asp  Ala Gly Pro Ser Leu  Leu Phe Ile 
    1010                 1015                 1020             


Thr Tyr  Ala Glu Ala Ile Ala  Asn Met Pro Ala Ser  Thr Phe Phe 
    1025                 1030                 1035             


Ala Ile  Ile Phe Phe Leu Met  Leu Ile Thr Leu Gly  Leu Asp Ser 
    1040                 1045                 1050             


Thr Phe  Ala Gly Leu Glu Gly  Val Ile Thr Ala Val  Leu Asp Glu 
    1055                 1060                 1065             


Phe Pro  His Val Trp Ala Lys  Arg Arg Glu Arg Phe  Val Leu Ala 
    1070                 1075                 1080             


Val Val  Ile Thr Cys Phe Phe  Gly Ser Leu Val Thr  Leu Thr Phe 
    1085                 1090                 1095             


Gly Gly  Ala Tyr Val Val Lys  Leu Leu Glu Glu Tyr  Ala Thr Gly 
    1100                 1105                 1110             


Pro Ala  Val Leu Thr Val Ala  Leu Ile Glu Ala Val  Ala Val Ser 
    1115                 1120                 1125             


Trp Phe  Tyr Gly Ile Thr Gln  Phe Cys Arg Asp Val  Lys Glu Met 
    1130                 1135                 1140             


Leu Gly  Phe Ser Pro Gly Trp  Phe Trp Arg Ile Cys  Trp Val Ala 
    1145                 1150                 1155             


Ile Ser  Pro Leu Phe Leu Leu  Phe Ile Ile Cys Ser  Phe Leu Met 
    1160                 1165                 1170             


Ser Pro  Pro Gln Leu Arg Leu  Phe Gln Tyr Asn Tyr  Pro Tyr Trp 
    1175                 1180                 1185             


Ser Ile  Ile Leu Gly Tyr Cys  Ile Gly Thr Ser Ser  Phe Ile Cys 
    1190                 1195                 1200             


Ile Pro  Thr Tyr Ile Ala Tyr  Arg Leu Ile Ile Thr  Pro Gly Thr 
    1205                 1210                 1215             


Phe Lys  Glu Arg Ile Ile Lys  Ser Ile Thr Pro Glu  Thr Pro Thr 
    1220                 1225                 1230             


Glu Ile  Pro Cys Gly Asp Ile  Arg Leu Asn Ala Val  
    1235                 1240                 1245 


<210>  30
<211>  1593
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ERN1(K599A)-Tyk2(C)-HA fusion protein

<400>  30

Met Pro Ala Arg Arg Leu Leu Leu Leu Leu Thr Leu Leu Leu Pro Gly 
1               5                   10                  15      


Leu Gly Ile Phe Gly Ser Thr Ser Thr Val Thr Leu Pro Glu Thr Leu 
            20                  25                  30          


Leu Phe Val Ser Thr Leu Asp Gly Ser Leu His Ala Val Ser Lys Arg 
        35                  40                  45              


Thr Gly Ser Ile Lys Trp Thr Leu Lys Glu Asp Pro Val Leu Gln Val 
    50                  55                  60                  


Pro Thr His Val Glu Glu Pro Ala Phe Leu Pro Asp Pro Asn Asp Gly 
65                  70                  75                  80  


Ser Leu Tyr Thr Leu Gly Ser Lys Asn Asn Glu Gly Leu Thr Lys Leu 
                85                  90                  95      


Pro Phe Thr Ile Pro Glu Leu Val Gln Ala Ser Pro Cys Arg Ser Ser 
            100                 105                 110         


Asp Gly Ile Leu Tyr Met Gly Lys Lys Gln Asp Ile Trp Tyr Val Ile 
        115                 120                 125             


Asp Leu Leu Thr Gly Glu Lys Gln Gln Thr Leu Ser Ser Ala Phe Ala 
    130                 135                 140                 


Asp Ser Leu Cys Pro Ser Thr Ser Leu Leu Tyr Leu Gly Arg Thr Glu 
145                 150                 155                 160 


Tyr Thr Ile Thr Met Tyr Asp Thr Lys Thr Arg Glu Leu Arg Trp Asn 
                165                 170                 175     


Ala Thr Tyr Phe Asp Tyr Ala Ala Ser Leu Pro Glu Asp Asp Val Asp 
            180                 185                 190         


Tyr Lys Met Ser His Phe Val Ser Asn Gly Asp Gly Leu Val Val Thr 
        195                 200                 205             


Val Asp Ser Glu Ser Gly Asp Val Leu Trp Ile Gln Asn Tyr Ala Ser 
    210                 215                 220                 


Pro Val Val Ala Phe Tyr Val Trp Gln Arg Glu Gly Leu Arg Lys Val 
225                 230                 235                 240 


Met His Ile Asn Val Ala Val Glu Thr Leu Arg Tyr Leu Thr Phe Met 
                245                 250                 255     


Ser Gly Glu Val Gly Arg Ile Thr Lys Trp Lys Tyr Pro Phe Pro Lys 
            260                 265                 270         


Glu Thr Glu Ala Lys Ser Lys Leu Thr Pro Thr Leu Tyr Val Gly Lys 
        275                 280                 285             


Tyr Ser Thr Ser Leu Tyr Ala Ser Pro Ser Met Val His Glu Gly Val 
    290                 295                 300                 


Ala Val Val Pro Arg Gly Ser Thr Leu Pro Leu Leu Glu Gly Pro Gln 
305                 310                 315                 320 


Thr Asp Gly Val Thr Ile Gly Asp Lys Gly Glu Cys Val Ile Thr Pro 
                325                 330                 335     


Ser Thr Asp Val Lys Phe Asp Pro Gly Leu Lys Ser Lys Asn Lys Leu 
            340                 345                 350         


Asn Tyr Leu Arg Asn Tyr Trp Leu Leu Ile Gly His His Glu Thr Pro 
        355                 360                 365             


Leu Ser Ala Ser Thr Lys Met Leu Glu Arg Phe Pro Asn Asn Leu Pro 
    370                 375                 380                 


Lys His Arg Glu Asn Val Ile Pro Ala Asp Ser Glu Lys Lys Ser Phe 
385                 390                 395                 400 


Glu Glu Val Ile Asn Leu Val Asp Gln Thr Ser Glu Asn Ala Pro Thr 
                405                 410                 415     


Thr Val Ser Arg Asp Val Glu Glu Lys Pro Ala His Ala Pro Ala Arg 
            420                 425                 430         


Pro Glu Ala Pro Val Asp Ser Met Leu Lys Asp Met Ala Thr Ile Ile 
        435                 440                 445             


Leu Ser Thr Phe Leu Leu Ile Gly Trp Val Ala Phe Ile Ile Thr Tyr 
    450                 455                 460                 


Pro Leu Ser Met His Gln Gln Gln Gln Leu Gln His Gln Gln Phe Gln 
465                 470                 475                 480 


Lys Glu Leu Glu Lys Ile Gln Leu Leu Gln Gln Gln Gln Gln Gln Leu 
                485                 490                 495     


Pro Phe His Pro Pro Gly Asp Thr Ala Gln Asp Gly Glu Leu Leu Asp 
            500                 505                 510         


Thr Ser Gly Pro Tyr Ser Glu Ser Ser Gly Thr Ser Ser Pro Ser Thr 
        515                 520                 525             


Ser Pro Arg Ala Ser Asn His Ser Leu Cys Ser Gly Ser Ser Ala Ser 
    530                 535                 540                 


Lys Ala Gly Ser Ser Pro Ser Leu Glu Gln Asp Asp Gly Asp Glu Glu 
545                 550                 555                 560 


Thr Ser Val Val Ile Val Gly Lys Ile Ser Phe Cys Pro Lys Asp Val 
                565                 570                 575     


Leu Gly His Gly Ala Glu Gly Thr Ile Val Tyr Arg Gly Met Phe Asp 
            580                 585                 590         


Asn Arg Asp Val Ala Val Ala Arg Ile Leu Pro Glu Cys Phe Ser Phe 
        595                 600                 605             


Ala Asp Arg Glu Val Gln Leu Leu Arg Glu Ser Asp Glu His Pro Asn 
    610                 615                 620                 


Val Ile Arg Tyr Phe Cys Thr Glu Lys Asp Arg Gln Phe Gln Tyr Ile 
625                 630                 635                 640 


Ala Ile Glu Leu Cys Ala Ala Thr Leu Gln Glu Tyr Val Glu Gln Lys 
                645                 650                 655     


Asp Phe Ala His Leu Gly Leu Glu Pro Ile Thr Leu Leu Gln Gln Thr 
            660                 665                 670         


Thr Ser Gly Leu Ala His Leu His Ser Leu Asn Ile Val His Arg Asp 
        675                 680                 685             


Leu Lys Pro His Asn Ile Leu Ile Ser Met Pro Asn Ala His Gly Lys 
    690                 695                 700                 


Ile Lys Ala Met Ile Ser Asp Phe Gly Leu Cys Lys Lys Leu Ala Val 
705                 710                 715                 720 


Gly Arg His Ser Phe Ser Arg Arg Ser Gly Val Pro Gly Thr Glu Gly 
                725                 730                 735     


Trp Ile Ala Pro Glu Met Leu Ser Glu Asp Cys Lys Glu Asn Pro Thr 
            740                 745                 750         


Tyr Thr Val Asp Ile Phe Ser Ala Gly Cys Val Phe Tyr Tyr Val Ile 
        755                 760                 765             


Ser Glu Gly Ser His Pro Phe Gly Lys Ser Leu Gln Arg Gln Ala Asn 
    770                 775                 780                 


Ile Leu Leu Gly Ala Cys Ser Leu Asp Cys Leu His Pro Glu Lys His 
785                 790                 795                 800 


Glu Asp Val Ile Ala Arg Glu Leu Ile Glu Lys Met Ile Ala Met Asp 
                805                 810                 815     


Pro Gln Lys Arg Pro Ser Ala Lys His Val Leu Lys His Pro Phe Phe 
            820                 825                 830         


Trp Ser Leu Glu Lys Gln Leu Gln Phe Phe Gln Asp Val Ser Asp Arg 
        835                 840                 845             


Ile Glu Lys Glu Ser Leu Asp Gly Pro Ile Val Lys Gln Leu Glu Arg 
    850                 855                 860                 


Gly Gly Arg Ala Val Val Lys Met Asp Trp Arg Glu Asn Ile Thr Val 
865                 870                 875                 880 


Pro Leu Gln Thr Asp Leu Arg Lys Phe Arg Thr Tyr Lys Gly Gly Ser 
                885                 890                 895     


Val Arg Asp Leu Leu Arg Ala Met Arg Asn Lys Lys His His Tyr Arg 
            900                 905                 910         


Glu Leu Pro Ala Glu Val Arg Glu Thr Leu Gly Ser Leu Pro Asp Asp 
        915                 920                 925             


Phe Val Cys Tyr Phe Thr Ser Arg Phe Pro His Leu Leu Ala His Thr 
    930                 935                 940                 


Tyr Arg Ala Met Glu Leu Cys Ser His Glu Arg Leu Phe Gln Pro Tyr 
945                 950                 955                 960 


Tyr Phe His Glu Pro Pro Glu Pro Gln Pro Pro Val Thr Pro Asp Ala 
                965                 970                 975     


Leu Ala Ala Ala Gly Gly Ser Ile Thr Gln Leu Ser His Leu Gly Gln 
            980                 985                 990         


Gly Thr Arg Thr Asn Val Tyr Glu  Gly Arg Leu Arg Val  Glu Gly Ser 
        995                 1000                 1005             


Gly Asp  Pro Glu Glu Gly Lys  Met Asp Asp Glu Asp  Pro Leu Val 
    1010                 1015                 1020             


Pro Gly  Arg Asp Arg Gly Gln  Glu Leu Arg Val Val  Leu Lys Val 
    1025                 1030                 1035             


Leu Asp  Pro Ser His His Asp  Ile Ala Leu Ala Phe  Tyr Glu Thr 
    1040                 1045                 1050             


Ala Ser  Leu Met Ser Gln Val  Ser His Thr His Leu  Ala Phe Val 
    1055                 1060                 1065             


His Gly  Val Cys Val Arg Gly  Pro Glu Asn Ser Met  Val Thr Glu 
    1070                 1075                 1080             


Tyr Val  Glu His Gly Pro Leu  Asp Val Trp Leu Arg  Arg Glu Arg 
    1085                 1090                 1095             


Gly His  Val Pro Met Ala Trp  Lys Met Val Val Ala  Gln Gln Leu 
    1100                 1105                 1110             


Ala Ser  Ala Leu Ser Tyr Leu  Glu Asn Lys Asn Leu  Val His Gly 
    1115                 1120                 1125             


Asn Val  Cys Gly Arg Asn Ile  Leu Leu Ala Arg Leu  Gly Leu Ala 
    1130                 1135                 1140             


Glu Gly  Thr Ser Pro Phe Ile  Lys Leu Ser Asp Pro  Gly Val Gly 
    1145                 1150                 1155             


Leu Gly  Ala Leu Ser Arg Glu  Glu Arg Val Glu Arg  Ile Pro Trp 
    1160                 1165                 1170             


Leu Ala  Pro Glu Cys Leu Pro  Gly Gly Ala Asn Ser  Leu Ser Thr 
    1175                 1180                 1185             


Ala Met  Asp Lys Trp Gly Phe  Gly Ala Thr Leu Leu  Glu Ile Cys 
    1190                 1195                 1200             


Phe Asp  Gly Glu Ala Pro Leu  Gln Ser Arg Ser Pro  Ser Glu Lys 
    1205                 1210                 1215             


Glu His  Phe Tyr Gln Arg Gln  His Arg Leu Pro Glu  Pro Ser Cys 
    1220                 1225                 1230             


Pro Gln  Leu Ala Thr Leu Thr  Ser Gln Cys Leu Thr  Tyr Glu Pro 
    1235                 1240                 1245             


Thr Gln  Arg Pro Ser Phe Arg  Thr Ile Leu Arg Asp  Leu Thr Arg 
    1250                 1255                 1260             


Val Gln  Pro His Asn Leu Ala  Asp Val Leu Thr Val  Asn Arg Asp 
    1265                 1270                 1275             


Ser Pro  Ala Val Gly Pro Thr  Thr Phe His Lys Arg  Tyr Leu Lys 
    1280                 1285                 1290             


Lys Ile  Arg Asp Leu Gly Glu  Gly His Phe Gly Lys  Val Ser Leu 
    1295                 1300                 1305             


Tyr Cys  Tyr Asp Pro Thr Asn  Asp Gly Thr Gly Glu  Met Val Ala 
    1310                 1315                 1320             


Val Lys  Ala Leu Lys Ala Asp  Cys Gly Pro Gln His  Arg Ser Gly 
    1325                 1330                 1335             


Trp Lys  Gln Glu Ile Asp Ile  Leu Arg Thr Leu Tyr  His Glu His 
    1340                 1345                 1350             


Ile Ile  Lys Tyr Lys Gly Cys  Cys Glu Asp Gln Gly  Glu Lys Ser 
    1355                 1360                 1365             


Leu Gln  Leu Val Met Glu Tyr  Val Pro Leu Gly Ser  Leu Arg Asp 
    1370                 1375                 1380             


Tyr Leu  Pro Arg His Ser Ile  Gly Leu Ala Gln Leu  Leu Leu Phe 
    1385                 1390                 1395             


Ala Gln  Gln Ile Cys Glu Gly  Met Ala Tyr Leu His  Ala His Asp 
    1400                 1405                 1410             


Tyr Ile  His Arg Asp Leu Ala  Ala Arg Asn Val Leu  Leu Asp Asn 
    1415                 1420                 1425             


Asp Arg  Leu Val Lys Ile Gly  Asp Phe Gly Leu Ala  Lys Ala Val 
    1430                 1435                 1440             


Pro Glu  Gly His Glu Tyr Tyr  Arg Val Arg Glu Asp  Gly Asp Ser 
    1445                 1450                 1455             


Pro Val  Phe Trp Tyr Ala Pro  Glu Cys Leu Lys Glu  Tyr Lys Phe 
    1460                 1465                 1470             


Tyr Tyr  Ala Ser Asp Val Trp  Ser Phe Gly Val Thr  Leu Tyr Glu 
    1475                 1480                 1485             


Leu Leu  Thr His Cys Asp Ser  Ser Gln Ser Pro Pro  Thr Lys Phe 
    1490                 1495                 1500             


Leu Glu  Leu Ile Gly Ile Ala  Gln Gly Gln Met Thr  Val Leu Arg 
    1505                 1510                 1515             


Leu Thr  Glu Leu Leu Glu Arg  Gly Glu Arg Leu Pro  Arg Pro Asp 
    1520                 1525                 1530             


Lys Cys  Pro Cys Glu Val Tyr  His Leu Met Lys Asn  Cys Trp Glu 
    1535                 1540                 1545             


Thr Glu  Ala Ser Phe Arg Pro  Thr Phe Glu Asn Leu  Ile Pro Ile 
    1550                 1555                 1560             


Leu Lys  Thr Val His Glu Lys  Tyr Gln Gly Gln Ala  Pro Ser Val 
    1565                 1570                 1575             


Phe Ser  Val Cys Leu Glu Tyr  Pro Tyr Asp Val Pro  Asp Tyr Ala 
    1580                 1585                 1590             


<210>  31
<211>  1595
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ERN1(D123P)-Tyk2(K)-HA fusion protein

<400>  31

Met Pro Ala Arg Arg Leu Leu Leu Leu Leu Thr Leu Leu Leu Pro Gly 
1               5                   10                  15      


Leu Gly Ile Phe Gly Ser Thr Ser Thr Val Thr Leu Pro Glu Thr Leu 
            20                  25                  30          


Leu Phe Val Ser Thr Leu Asp Gly Ser Leu His Ala Val Ser Lys Arg 
        35                  40                  45              


Thr Gly Ser Ile Lys Trp Thr Leu Lys Glu Asp Pro Val Leu Gln Val 
    50                  55                  60                  


Pro Thr His Val Glu Glu Pro Ala Phe Leu Pro Asp Pro Asn Asp Gly 
65                  70                  75                  80  


Ser Leu Tyr Thr Leu Gly Ser Lys Asn Asn Glu Gly Leu Thr Lys Leu 
                85                  90                  95      


Pro Phe Thr Ile Pro Glu Leu Val Gln Ala Ser Pro Cys Arg Ser Ser 
            100                 105                 110         


Asp Gly Ile Leu Tyr Met Gly Lys Lys Gln Pro Ile Trp Tyr Val Ile 
        115                 120                 125             


Asp Leu Leu Thr Gly Glu Lys Gln Gln Thr Leu Ser Ser Ala Phe Ala 
    130                 135                 140                 


Asp Ser Leu Cys Pro Ser Thr Ser Leu Leu Tyr Leu Gly Arg Thr Glu 
145                 150                 155                 160 


Tyr Thr Ile Thr Met Tyr Asp Thr Lys Thr Arg Glu Leu Arg Trp Asn 
                165                 170                 175     


Ala Thr Tyr Phe Asp Tyr Ala Ala Ser Leu Pro Glu Asp Asp Val Asp 
            180                 185                 190         


Tyr Lys Met Ser His Phe Val Ser Asn Gly Asp Gly Leu Val Val Thr 
        195                 200                 205             


Val Asp Ser Glu Ser Gly Asp Val Leu Trp Ile Gln Asn Tyr Ala Ser 
    210                 215                 220                 


Pro Val Val Ala Phe Tyr Val Trp Gln Arg Glu Gly Leu Arg Lys Val 
225                 230                 235                 240 


Met His Ile Asn Val Ala Val Glu Thr Leu Arg Tyr Leu Thr Phe Met 
                245                 250                 255     


Ser Gly Glu Val Gly Arg Ile Thr Lys Trp Lys Tyr Pro Phe Pro Lys 
            260                 265                 270         


Glu Thr Glu Ala Lys Ser Lys Leu Thr Pro Thr Leu Tyr Val Gly Lys 
        275                 280                 285             


Tyr Ser Thr Ser Leu Tyr Ala Ser Pro Ser Met Val His Glu Gly Val 
    290                 295                 300                 


Ala Val Val Pro Arg Gly Ser Thr Leu Pro Leu Leu Glu Gly Pro Gln 
305                 310                 315                 320 


Thr Asp Gly Val Thr Ile Gly Asp Lys Gly Glu Cys Val Ile Thr Pro 
                325                 330                 335     


Ser Thr Asp Val Lys Phe Asp Pro Gly Leu Lys Ser Lys Asn Lys Leu 
            340                 345                 350         


Asn Tyr Leu Arg Asn Tyr Trp Leu Leu Ile Gly His His Glu Thr Pro 
        355                 360                 365             


Leu Ser Ala Ser Thr Lys Met Leu Glu Arg Phe Pro Asn Asn Leu Pro 
    370                 375                 380                 


Lys His Arg Glu Asn Val Ile Pro Ala Asp Ser Glu Lys Lys Ser Phe 
385                 390                 395                 400 


Glu Glu Val Ile Asn Leu Val Asp Gln Thr Ser Glu Asn Ala Pro Thr 
                405                 410                 415     


Thr Val Ser Arg Asp Val Glu Glu Lys Pro Ala His Ala Pro Ala Arg 
            420                 425                 430         


Pro Glu Ala Pro Val Asp Ser Met Leu Lys Asp Met Ala Thr Ile Ile 
        435                 440                 445             


Leu Ser Thr Phe Leu Leu Ile Gly Trp Val Ala Phe Ile Ile Thr Tyr 
    450                 455                 460                 


Pro Leu Ser Met His Gln Gln Gln Gln Leu Gln His Gln Gln Phe Gln 
465                 470                 475                 480 


Lys Glu Leu Glu Lys Ile Gln Leu Leu Gln Gln Gln Gln Gln Gln Leu 
                485                 490                 495     


Pro Phe His Pro Pro Gly Asp Thr Ala Gln Asp Gly Glu Leu Leu Asp 
            500                 505                 510         


Thr Ser Gly Pro Tyr Ser Glu Ser Ser Gly Thr Ser Ser Pro Ser Thr 
        515                 520                 525             


Ser Pro Arg Ala Ser Asn His Ser Leu Cys Ser Gly Ser Ser Ala Ser 
    530                 535                 540                 


Lys Ala Gly Ser Ser Pro Ser Leu Glu Gln Asp Asp Gly Asp Glu Glu 
545                 550                 555                 560 


Thr Ser Val Val Ile Val Gly Lys Ile Ser Phe Cys Pro Lys Asp Val 
                565                 570                 575     


Leu Gly His Gly Ala Glu Gly Thr Ile Val Tyr Arg Gly Met Phe Asp 
            580                 585                 590         


Asn Arg Asp Val Ala Val Lys Arg Ile Leu Pro Glu Cys Phe Ser Phe 
        595                 600                 605             


Ala Asp Arg Glu Val Gln Leu Leu Arg Glu Ser Asp Glu His Pro Asn 
    610                 615                 620                 


Val Ile Arg Tyr Phe Cys Thr Glu Lys Asp Arg Gln Phe Gln Tyr Ile 
625                 630                 635                 640 


Ala Ile Glu Leu Cys Ala Ala Thr Leu Gln Glu Tyr Val Glu Gln Lys 
                645                 650                 655     


Asp Phe Ala His Leu Gly Leu Glu Pro Ile Thr Leu Leu Gln Gln Thr 
            660                 665                 670         


Thr Ser Gly Leu Ala His Leu His Ser Leu Asn Ile Val His Arg Asp 
        675                 680                 685             


Leu Lys Pro His Asn Ile Leu Ile Ser Met Pro Asn Ala His Gly Lys 
    690                 695                 700                 


Ile Lys Ala Met Ile Ser Asp Phe Gly Leu Cys Lys Lys Leu Ala Val 
705                 710                 715                 720 


Gly Arg His Ser Phe Ser Arg Arg Ser Gly Val Pro Gly Thr Glu Gly 
                725                 730                 735     


Trp Ile Ala Pro Glu Met Leu Ser Glu Asp Cys Lys Glu Asn Pro Thr 
            740                 745                 750         


Tyr Thr Val Asp Ile Phe Ser Ala Gly Cys Val Phe Tyr Tyr Val Ile 
        755                 760                 765             


Ser Glu Gly Ser His Pro Phe Gly Lys Ser Leu Gln Arg Gln Ala Asn 
    770                 775                 780                 


Ile Leu Leu Gly Ala Cys Ser Leu Asp Cys Leu His Pro Glu Lys His 
785                 790                 795                 800 


Glu Asp Val Ile Ala Arg Glu Leu Ile Glu Lys Met Ile Ala Met Asp 
                805                 810                 815     


Pro Gln Lys Arg Pro Ser Ala Lys His Val Leu Lys His Pro Phe Phe 
            820                 825                 830         


Trp Ser Leu Glu Lys Gln Leu Gln Phe Phe Gln Asp Val Ser Asp Arg 
        835                 840                 845             


Ile Glu Lys Glu Ser Leu Asp Gly Pro Ile Val Lys Gln Leu Glu Arg 
    850                 855                 860                 


Gly Gly Arg Ala Val Val Lys Met Asp Trp Arg Glu Asn Ile Thr Val 
865                 870                 875                 880 


Pro Leu Gln Thr Asp Leu Arg Lys Phe Arg Thr Tyr Lys Gly Gly Ser 
                885                 890                 895     


Val Arg Asp Leu Leu Arg Ala Met Arg Asn Lys Lys His His Tyr Arg 
            900                 905                 910         


Glu Leu Pro Ala Glu Val Arg Glu Thr Leu Gly Ser Leu Pro Asp Asp 
        915                 920                 925             


Phe Val Cys Tyr Phe Thr Ser Arg Phe Pro His Leu Leu Ala His Thr 
    930                 935                 940                 


Tyr Arg Ala Met Glu Leu Cys Ser His Glu Arg Leu Phe Gln Pro Tyr 
945                 950                 955                 960 


Tyr Phe His Glu Pro Pro Glu Pro Gln Pro Pro Val Thr Pro Asp Ala 
                965                 970                 975     


Leu Ala Ser Ala Ala Ala Gly Gly Ser Ile Thr Gln Leu Ser His Leu 
            980                 985                 990         


Gly Gln Gly Thr Arg Thr Asn Val  Tyr Glu Gly Arg Leu  Arg Val Glu 
        995                 1000                 1005             


Gly Ser  Gly Asp Pro Glu Glu  Gly Lys Met Asp Asp  Glu Asp Pro 
    1010                 1015                 1020             


Leu Val  Pro Gly Arg Asp Arg  Gly Gln Glu Leu Arg  Val Val Leu 
    1025                 1030                 1035             


Lys Val  Leu Asp Pro Ser His  His Asp Ile Ala Leu  Ala Phe Tyr 
    1040                 1045                 1050             


Glu Thr  Ala Ser Leu Met Ser  Gln Val Ser His Thr  His Leu Ala 
    1055                 1060                 1065             


Phe Val  His Gly Val Cys Val  Arg Gly Pro Glu Asn  Ser Met Val 
    1070                 1075                 1080             


Thr Glu  Tyr Val Glu His Gly  Pro Leu Asp Val Trp  Leu Arg Arg 
    1085                 1090                 1095             


Glu Arg  Gly His Val Pro Met  Ala Trp Lys Met Val  Val Ala Gln 
    1100                 1105                 1110             


Gln Leu  Ala Ser Ala Leu Ser  Tyr Leu Glu Asn Lys  Asn Leu Val 
    1115                 1120                 1125             


His Gly  Asn Val Cys Gly Arg  Asn Ile Leu Leu Ala  Arg Leu Gly 
    1130                 1135                 1140             


Leu Ala  Glu Gly Thr Ser Pro  Phe Ile Lys Leu Ser  Asp Pro Gly 
    1145                 1150                 1155             


Val Gly  Leu Gly Ala Leu Ser  Arg Glu Glu Arg Val  Glu Arg Ile 
    1160                 1165                 1170             


Pro Trp  Leu Ala Pro Glu Cys  Leu Pro Gly Gly Ala  Asn Ser Leu 
    1175                 1180                 1185             


Ser Thr  Ala Met Asp Lys Trp  Gly Phe Gly Ala Thr  Leu Leu Glu 
    1190                 1195                 1200             


Ile Cys  Phe Asp Gly Glu Ala  Pro Leu Gln Ser Arg  Ser Pro Ser 
    1205                 1210                 1215             


Glu Lys  Glu His Phe Tyr Gln  Arg Gln His Arg Leu  Pro Glu Pro 
    1220                 1225                 1230             


Ser Cys  Pro Gln Leu Ala Thr  Leu Thr Ser Gln Cys  Leu Thr Tyr 
    1235                 1240                 1245             


Glu Pro  Thr Gln Arg Pro Ser  Phe Arg Thr Ile Leu  Arg Asp Leu 
    1250                 1255                 1260             


Thr Arg  Val Gln Pro His Asn  Leu Ala Asp Val Leu  Thr Val Asn 
    1265                 1270                 1275             


Arg Asp  Ser Pro Ala Val Gly  Pro Thr Thr Phe His  Lys Arg Tyr 
    1280                 1285                 1290             


Leu Lys  Lys Ile Arg Asp Leu  Gly Glu Gly His Phe  Gly Lys Val 
    1295                 1300                 1305             


Ser Leu  Tyr Cys Tyr Asp Pro  Thr Asn Asp Gly Thr  Gly Glu Met 
    1310                 1315                 1320             


Val Ala  Val Lys Ala Leu Lys  Ala Asp Cys Gly Pro  Gln His Arg 
    1325                 1330                 1335             


Ser Gly  Trp Lys Gln Glu Ile  Asp Ile Leu Arg Thr  Leu Tyr His 
    1340                 1345                 1350             


Glu His  Ile Ile Lys Tyr Lys  Gly Cys Cys Glu Asp  Gln Gly Glu 
    1355                 1360                 1365             


Lys Ser  Leu Gln Leu Val Met  Glu Tyr Val Pro Leu  Gly Ser Leu 
    1370                 1375                 1380             


Arg Asp  Tyr Leu Pro Arg His  Ser Ile Gly Leu Ala  Gln Leu Leu 
    1385                 1390                 1395             


Leu Phe  Ala Gln Gln Ile Cys  Glu Gly Met Ala Tyr  Leu His Ala 
    1400                 1405                 1410             


His Asp  Tyr Ile His Arg Asp  Leu Ala Ala Arg Asn  Val Leu Leu 
    1415                 1420                 1425             


Asp Asn  Asp Arg Leu Val Lys  Ile Gly Asp Phe Gly  Leu Ala Lys 
    1430                 1435                 1440             


Ala Val  Pro Glu Gly His Glu  Tyr Tyr Arg Val Arg  Glu Asp Gly 
    1445                 1450                 1455             


Asp Ser  Pro Val Phe Trp Tyr  Ala Pro Glu Cys Leu  Lys Glu Tyr 
    1460                 1465                 1470             


Lys Phe  Tyr Tyr Ala Ser Asp  Val Trp Ser Phe Gly  Val Thr Leu 
    1475                 1480                 1485             


Tyr Glu  Leu Leu Thr His Cys  Asp Ser Ser Gln Ser  Pro Pro Thr 
    1490                 1495                 1500             


Lys Phe  Leu Glu Leu Ile Gly  Ile Ala Gln Gly Gln  Met Thr Val 
    1505                 1510                 1515             


Leu Arg  Leu Thr Glu Leu Leu  Glu Arg Gly Glu Arg  Leu Pro Arg 
    1520                 1525                 1530             


Pro Asp  Lys Cys Pro Cys Glu  Val Tyr His Leu Met  Lys Asn Cys 
    1535                 1540                 1545             


Trp Glu  Thr Glu Ala Ser Phe  Arg Pro Thr Phe Glu  Asn Leu Ile 
    1550                 1555                 1560             


Pro Ile  Leu Lys Thr Val His  Glu Lys Tyr Gln Gly  Gln Ala Pro 
    1565                 1570                 1575             


Ser Val  Phe Ser Val Cys Leu  Glu Tyr Pro Tyr Asp  Val Pro Asp 
    1580                 1585                 1590             


Tyr Ala  
    1595 


<210>  32
<211>  309
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  flag tag-gp130-VAMP1 construct

<400>  32

Met Asp Tyr Lys Asp Asp Asp Asp Lys Ile Ser Thr Val Val His Ser 
1               5                   10                  15      


Gly Tyr Arg His Gln Val Pro Ser Val Gln Val Phe Ser Arg Ser Glu 
            20                  25                  30          


Ser Thr Gln Pro Leu Leu Asp Ser Glu Glu Arg Pro Glu Asp Leu Gln 
        35                  40                  45              


Leu Val Asp His Val Asp Gly Gly Asp Gly Ile Leu Pro Arg Gln Gln 
    50                  55                  60                  


Tyr Phe Lys Gln Asn Cys Ser Gln His Glu Ser Ser Pro Asp Ile Ser 
65                  70                  75                  80  


His Phe Glu Arg Ser Lys Gln Val Ser Ser Val Asn Glu Glu Asp Phe 
                85                  90                  95      


Val Arg Leu Lys Gln Gln Ile Ser Asp His Ile Ser Gln Ser Cys Gly 
            100                 105                 110         


Ser Gly Gln Met Lys Met Phe Gln Glu Val Ser Ala Ala Asp Ala Phe 
        115                 120                 125             


Gly Pro Gly Thr Glu Gly Gln Val Glu Arg Phe Glu Thr Val Gly Met 
    130                 135                 140                 


Glu Ala Ala Thr Asp Glu Gly Met Pro Lys Ser Tyr Leu Pro Gln Thr 
145                 150                 155                 160 


Val Arg Gln Gly Gly Tyr Met Pro Gln Gly Gly Ser Glu Leu Ser Thr 
                165                 170                 175     


Ser Leu Tyr Lys Lys Val Gly Met Ser Ala Pro Ala Gln Pro Pro Ala 
            180                 185                 190         


Glu Gly Thr Glu Gly Thr Ala Pro Gly Gly Gly Pro Pro Gly Pro Pro 
        195                 200                 205             


Pro Asn Met Thr Ser Asn Arg Arg Leu Gln Gln Thr Gln Ala Gln Val 
    210                 215                 220                 


Glu Glu Val Val Asp Ile Ile Arg Val Asn Val Asp Lys Val Leu Glu 
225                 230                 235                 240 


Arg Asp Gln Lys Leu Ser Glu Leu Asp Asp Arg Ala Asp Ala Leu Gln 
                245                 250                 255     


Ala Gly Ala Ser Gln Phe Glu Ser Ser Ala Ala Lys Leu Lys Arg Lys 
            260                 265                 270         


Tyr Trp Trp Lys Asn Cys Lys Met Met Ile Met Leu Gly Ala Ile Cys 
        275                 280                 285             


Ala Ile Ile Val Val Val Ile Val Ser Lys Tyr Arg Cys Pro Thr Phe 
    290                 295                 300                 


Leu Tyr Lys Val Val 
305                 


<210>  33
<211>  308
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  flag tag-gp130-VAMP2 fusion construct

<400>  33

Met Asp Tyr Lys Asp Asp Asp Asp Lys Ile Ser Thr Val Val His Ser 
1               5                   10                  15      


Gly Tyr Arg His Gln Val Pro Ser Val Gln Val Phe Ser Arg Ser Glu 
            20                  25                  30          


Ser Thr Gln Pro Leu Leu Asp Ser Glu Glu Arg Pro Glu Asp Leu Gln 
        35                  40                  45              


Leu Val Asp His Val Asp Gly Gly Asp Gly Ile Leu Pro Arg Gln Gln 
    50                  55                  60                  


Tyr Phe Lys Gln Asn Cys Ser Gln His Glu Ser Ser Pro Asp Ile Ser 
65                  70                  75                  80  


His Phe Glu Arg Ser Lys Gln Val Ser Ser Val Asn Glu Glu Asp Phe 
                85                  90                  95      


Val Arg Leu Lys Gln Gln Ile Ser Asp His Ile Ser Gln Ser Cys Gly 
            100                 105                 110         


Ser Gly Gln Met Lys Met Phe Gln Glu Val Ser Ala Ala Asp Ala Phe 
        115                 120                 125             


Gly Pro Gly Thr Glu Gly Gln Val Glu Arg Phe Glu Thr Val Gly Met 
    130                 135                 140                 


Glu Ala Ala Thr Asp Glu Gly Met Pro Lys Ser Tyr Leu Pro Gln Thr 
145                 150                 155                 160 


Val Arg Gln Gly Gly Tyr Met Pro Gln Gly Gly Ser Glu Leu Ser Thr 
                165                 170                 175     


Ser Leu Tyr Lys Lys Val Gly Met Ser Ala Thr Ala Ala Thr Ala Pro 
            180                 185                 190         


Pro Ala Ala Pro Ala Gly Glu Gly Gly Pro Pro Ala Pro Pro Pro Asn 
        195                 200                 205             


Leu Thr Ser Asn Arg Arg Leu Gln Gln Thr Gln Ala Gln Val Asp Glu 
    210                 215                 220                 


Val Val Asp Ile Met Arg Val Asn Val Asp Lys Val Leu Glu Arg Asp 
225                 230                 235                 240 


Gln Lys Leu Ser Glu Leu Asp Asp Arg Ala Asp Ala Leu Gln Ala Gly 
                245                 250                 255     


Ala Ser Gln Phe Glu Thr Ser Ala Ala Lys Leu Lys Arg Lys Tyr Trp 
            260                 265                 270         


Trp Lys Asn Leu Lys Met Met Ile Ile Leu Gly Val Ile Cys Ala Ile 
        275                 280                 285             


Ile Leu Ile Ile Ile Ile Val Tyr Phe Ser Thr Tyr Pro Thr Phe Leu 
    290                 295                 300                 


Tyr Lys Val Val 
305             


<210>  34
<211>  617
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  flag-tag-ERN1cyt-gp130 fusion construct

<400>  34

Met Asp Tyr Lys Asp Asp Asp Asp Lys Ile Ser Glu Phe Phe Cys Pro 
1               5                   10                  15      


Lys Asp Val Leu Gly His Gly Ala Glu Gly Thr Ile Val Tyr Arg Gly 
            20                  25                  30          


Met Phe Asp Asn Arg Asp Val Ala Val Lys Arg Ile Leu Pro Glu Cys 
        35                  40                  45              


Phe Ser Phe Ala Asp Arg Glu Val Gln Leu Leu Arg Glu Ser Asp Glu 
    50                  55                  60                  


His Pro Asn Val Ile Arg Tyr Phe Cys Thr Glu Lys Asp Arg Gln Phe 
65                  70                  75                  80  


Gln Tyr Ile Ala Ile Glu Leu Cys Ala Ala Thr Leu Gln Glu Tyr Val 
                85                  90                  95      


Glu Gln Lys Asp Phe Ala His Leu Gly Leu Glu Pro Ile Thr Leu Leu 
            100                 105                 110         


Gln Gln Thr Thr Ser Gly Leu Ala His Leu His Ser Leu Asn Ile Val 
        115                 120                 125             


His Arg Asp Leu Lys Pro His Asn Ile Leu Ile Ser Met Pro Asn Ala 
    130                 135                 140                 


His Gly Lys Ile Lys Ala Met Ile Ser Asp Phe Gly Leu Cys Lys Lys 
145                 150                 155                 160 


Leu Ala Val Gly Arg His Ser Phe Ser Arg Arg Ser Gly Val Pro Gly 
                165                 170                 175     


Thr Glu Gly Trp Ile Ala Pro Glu Met Leu Ser Glu Asp Cys Lys Glu 
            180                 185                 190         


Asn Pro Thr Tyr Thr Val Asp Ile Phe Ser Ala Gly Cys Val Phe Tyr 
        195                 200                 205             


Tyr Val Ile Ser Glu Gly Ser His Pro Phe Gly Lys Ser Leu Gln Arg 
    210                 215                 220                 


Gln Ala Asn Ile Leu Leu Gly Ala Cys Ser Leu Asp Cys Leu His Pro 
225                 230                 235                 240 


Glu Lys His Glu Asp Val Ile Ala Arg Glu Leu Ile Glu Lys Met Ile 
                245                 250                 255     


Ala Met Asp Pro Gln Lys Arg Pro Ser Ala Lys His Val Leu Lys His 
            260                 265                 270         


Pro Phe Phe Trp Ser Leu Glu Lys Gln Leu Gln Phe Phe Gln Asp Val 
        275                 280                 285             


Ser Asp Arg Ile Glu Lys Glu Ser Leu Asp Gly Pro Ile Val Lys Gln 
    290                 295                 300                 


Leu Glu Arg Gly Gly Arg Ala Val Val Lys Met Asp Trp Arg Glu Asn 
305                 310                 315                 320 


Ile Thr Val Pro Leu Gln Thr Asp Leu Arg Lys Phe Arg Thr Tyr Lys 
                325                 330                 335     


Gly Gly Ser Val Arg Asp Leu Leu Arg Ala Met Arg Asn Lys Lys His 
            340                 345                 350         


His Tyr Arg Glu Leu Pro Ala Glu Val Arg Glu Thr Leu Gly Ser Leu 
        355                 360                 365             


Pro Asp Asp Phe Val Cys Tyr Phe Thr Ser Arg Phe Pro His Leu Leu 
    370                 375                 380                 


Ala His Thr Tyr Arg Ala Met Glu Leu Cys Ser His Glu Arg Leu Phe 
385                 390                 395                 400 


Gln Pro Tyr Tyr Phe His Glu Pro Pro Glu Pro Gln Pro Pro Val Thr 
                405                 410                 415     


Pro Asp Ala Leu Pro Ser Arg Gly Ser Gly Gly Ser Gly Gly Ser Thr 
            420                 425                 430         


Val Val His Ser Gly Tyr Arg His Gln Val Pro Ser Val Gln Val Phe 
        435                 440                 445             


Ser Arg Ser Glu Ser Thr Gln Pro Leu Leu Asp Ser Glu Glu Arg Pro 
    450                 455                 460                 


Glu Asp Leu Gln Leu Val Asp His Val Asp Gly Gly Asp Gly Ile Leu 
465                 470                 475                 480 


Pro Arg Gln Gln Tyr Phe Lys Gln Asn Cys Ser Gln His Glu Ser Ser 
                485                 490                 495     


Pro Asp Ile Ser His Phe Glu Arg Ser Lys Gln Val Ser Ser Val Asn 
            500                 505                 510         


Glu Glu Asp Phe Val Arg Leu Lys Gln Gln Ile Ser Asp His Ile Ser 
        515                 520                 525             


Gln Ser Cys Gly Ser Gly Gln Met Lys Met Phe Gln Glu Val Ser Ala 
    530                 535                 540                 


Ala Asp Ala Phe Gly Pro Gly Thr Glu Gly Gln Val Glu Arg Phe Glu 
545                 550                 555                 560 


Thr Val Gly Met Glu Ala Ala Thr Asp Glu Gly Met Pro Lys Ser Tyr 
                565                 570                 575     


Leu Pro Gln Thr Val Arg Gln Gly Gly Tyr Met Pro Gln Gly Gly Ser 
            580                 585                 590         


Glu Leu Ser Thr Ser Leu Tyr Lys Lys Ala Gly Tyr Leu Pro Gln Thr 
        595                 600                 605             


Val Arg Gln Gly Gly Tyr Met Pro Gln 
    610                 615         


<210>  35
<211>  645
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  flag tag-gp130-RTp51 fusion construct

<400>  35

Met Asp Tyr Lys Asp Asp Asp Asp Lys Ile Ser Thr Val Val His Ser 
1               5                   10                  15      


Gly Tyr Arg His Gln Val Pro Ser Val Gln Val Phe Ser Arg Ser Glu 
            20                  25                  30          


Ser Thr Gln Pro Leu Leu Asp Ser Glu Glu Arg Pro Glu Asp Leu Gln 
        35                  40                  45              


Leu Val Asp His Val Asp Gly Gly Asp Gly Ile Leu Pro Arg Gln Gln 
    50                  55                  60                  


Tyr Phe Lys Gln Asn Cys Ser Gln His Glu Ser Ser Pro Asp Ile Ser 
65                  70                  75                  80  


His Phe Glu Arg Ser Lys Gln Val Ser Ser Val Asn Glu Glu Asp Phe 
                85                  90                  95      


Val Arg Leu Lys Gln Gln Ile Ser Asp His Ile Ser Gln Ser Cys Gly 
            100                 105                 110         


Ser Gly Gln Met Lys Met Phe Gln Glu Val Ser Ala Ala Asp Ala Phe 
        115                 120                 125             


Gly Pro Gly Thr Glu Gly Gln Val Glu Arg Phe Glu Thr Val Gly Met 
    130                 135                 140                 


Glu Ala Ala Thr Asp Glu Gly Met Pro Lys Ser Tyr Leu Pro Gln Thr 
145                 150                 155                 160 


Val Arg Gln Gly Gly Tyr Met Pro Gln Gly Gly Ser Glu Leu Ser Thr 
                165                 170                 175     


Ser Leu Tyr Lys Lys Ala Gly Tyr Leu Pro Gln Thr Val Arg Gln Gly 
            180                 185                 190         


Gly Tyr Met Pro Gln Gly Gly Ser Glu Phe Gly Ser Ser Pro Ile Ser 
        195                 200                 205             


Pro Ile Glu Thr Val Pro Val Lys Leu Lys Pro Gly Met Asp Gly Pro 
    210                 215                 220                 


Lys Val Lys Gln Trp Pro Leu Thr Glu Glu Lys Ile Lys Ala Leu Val 
225                 230                 235                 240 


Glu Ile Cys Thr Glu Met Glu Lys Glu Gly Lys Ile Ser Lys Ile Gly 
                245                 250                 255     


Pro Glu Asn Pro Tyr Asn Thr Pro Val Phe Ala Ile Lys Lys Lys Asp 
            260                 265                 270         


Ser Thr Lys Trp Arg Lys Leu Val Asp Phe Arg Glu Leu Asn Lys Arg 
        275                 280                 285             


Thr Gln Asp Phe Trp Glu Val Gln Leu Gly Ile Pro His Pro Ala Gly 
    290                 295                 300                 


Leu Lys Gln Lys Lys Ser Val Thr Val Leu Asp Val Gly Asp Ala Tyr 
305                 310                 315                 320 


Phe Ser Val Pro Leu Asp Lys Asp Phe Arg Lys Tyr Thr Ala Phe Thr 
                325                 330                 335     


Ile Pro Ser Ile Asn Asn Glu Thr Pro Gly Ile Arg Tyr Gln Tyr Asn 
            340                 345                 350         


Val Leu Pro Gln Gly Trp Lys Gly Ser Pro Ala Ile Phe Gln Cys Ser 
        355                 360                 365             


Met Thr Lys Ile Leu Glu Pro Phe Arg Lys Gln Asn Pro Asp Ile Val 
    370                 375                 380                 


Ile Tyr Gln Tyr Met Asp Asp Leu Tyr Val Gly Ser Asp Leu Glu Ile 
385                 390                 395                 400 


Gly Gln His Arg Thr Lys Ile Glu Glu Leu Arg Gln His Leu Leu Arg 
                405                 410                 415     


Trp Gly Phe Thr Thr Pro Asp Lys Lys His Gln Lys Glu Pro Pro Phe 
            420                 425                 430         


Leu Trp Met Gly Tyr Glu Leu His Pro Asp Lys Trp Thr Val Gln Pro 
        435                 440                 445             


Ile Val Leu Pro Glu Lys Asp Ser Trp Thr Val Asn Asp Ile Gln Lys 
    450                 455                 460                 


Leu Val Gly Lys Leu Asn Trp Ala Ser Gln Ile Tyr Ala Gly Ile Lys 
465                 470                 475                 480 


Val Arg Gln Leu Cys Lys Leu Leu Arg Gly Thr Lys Ala Leu Thr Glu 
                485                 490                 495     


Val Val Pro Leu Thr Glu Glu Ala Glu Leu Glu Leu Ala Glu Asn Arg 
            500                 505                 510         


Glu Ile Leu Lys Glu Pro Val His Gly Val Tyr Tyr Asp Pro Ser Lys 
        515                 520                 525             


Asp Leu Ile Ala Glu Ile Gln Lys Gln Gly Gln Gly Gln Trp Thr Tyr 
    530                 535                 540                 


Gln Ile Tyr Gln Glu Pro Phe Lys Asn Leu Lys Thr Gly Lys Tyr Ala 
545                 550                 555                 560 


Arg Met Lys Gly Ala His Thr Asn Asp Val Lys Gln Leu Thr Glu Ala 
                565                 570                 575     


Val Gln Lys Ile Ala Thr Glu Ser Ile Val Ile Trp Gly Lys Thr Pro 
            580                 585                 590         


Lys Phe Lys Leu Pro Ile Gln Lys Glu Thr Trp Glu Ala Trp Trp Thr 
        595                 600                 605             


Glu Tyr Trp Gln Ala Thr Trp Ile Pro Glu Trp Glu Phe Val Asn Thr 
    610                 615                 620                 


Pro Pro Leu Val Lys Leu Trp Tyr Gln Leu Glu Lys Glu Pro Ile Ile 
625                 630                 635                 640 


Gly Ala Glu Thr Phe 
                645 


<210>  36
<211>  174
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  polypeptide encoded by pMG1 plasmid

<400>  36

Met Asp Tyr Lys Asp Asp Asp Asp Lys Ile Ser Thr Val Val His Ser 
1               5                   10                  15      


Gly Tyr Arg His Gln Val Pro Ser Val Gln Val Phe Ser Arg Ser Glu 
            20                  25                  30          


Ser Thr Gln Pro Leu Leu Asp Ser Glu Glu Arg Pro Glu Asp Leu Gln 
        35                  40                  45              


Leu Val Asp His Val Asp Gly Gly Asp Gly Ile Leu Pro Arg Gln Gln 
    50                  55                  60                  


Tyr Phe Lys Gln Asn Cys Ser Gln His Glu Ser Ser Pro Asp Ile Ser 
65                  70                  75                  80  


His Phe Glu Arg Ser Lys Gln Val Ser Ser Val Asn Glu Glu Asp Phe 
                85                  90                  95      


Val Arg Leu Lys Gln Gln Ile Ser Asp His Ile Ser Gln Ser Cys Gly 
            100                 105                 110         


Ser Gly Gln Met Lys Met Phe Gln Glu Val Ser Ala Ala Asp Ala Phe 
        115                 120                 125             


Gly Pro Gly Thr Glu Gly Gln Val Glu Arg Phe Glu Thr Val Gly Met 
    130                 135                 140                 


Glu Ala Ala Thr Asp Glu Gly Met Pro Lys Ser Tyr Leu Pro Gln Thr 
145                 150                 155                 160 


Val Arg Gln Gly Gly Tyr Met Pro Gln Gly Gly Ser Glu Phe 
                165                 170                 


<210>  37
<211>  202
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  polypeptide encoded by pMG2 plasmid

<400>  37

Met Asp Tyr Lys Asp Asp Asp Asp Lys Ile Ser Thr Val Val His Ser 
1               5                   10                  15      


Gly Tyr Arg His Gln Val Pro Ser Val Gln Val Phe Ser Arg Ser Glu 
            20                  25                  30          


Ser Thr Gln Pro Leu Leu Asp Ser Glu Glu Arg Pro Glu Asp Leu Gln 
        35                  40                  45              


Leu Val Asp His Val Asp Gly Gly Asp Gly Ile Leu Pro Arg Gln Gln 
    50                  55                  60                  


Tyr Phe Lys Gln Asn Cys Ser Gln His Glu Ser Ser Pro Asp Ile Ser 
65                  70                  75                  80  


His Phe Glu Arg Ser Lys Gln Val Ser Ser Val Asn Glu Glu Asp Phe 
                85                  90                  95      


Val Arg Leu Lys Gln Gln Ile Ser Asp His Ile Ser Gln Ser Cys Gly 
            100                 105                 110         


Ser Gly Gln Met Lys Met Phe Gln Glu Val Ser Ala Ala Asp Ala Phe 
        115                 120                 125             


Gly Pro Gly Thr Glu Gly Gln Val Glu Arg Phe Glu Thr Val Gly Met 
    130                 135                 140                 


Glu Ala Ala Thr Asp Glu Gly Met Pro Lys Ser Tyr Leu Pro Gln Thr 
145                 150                 155                 160 


Val Arg Gln Gly Gly Tyr Met Pro Gln Gly Gly Ser Glu Leu Ser Thr 
                165                 170                 175     


Ser Leu Tyr Lys Lys Ala Gly Tyr Leu Pro Gln Thr Val Arg Gln Gly 
            180                 185                 190         


Gly Tyr Met Pro Gln Gly Gly Ser Glu Phe 
        195                 200         


