                               SEQUENCE LISTING

<110> CODEXIS, INC 
 
<120> GENE SHUFFLING METHODS

<130> CDXSP016WO

<140>
<141>

<150> 61/611,484
<151> 2012-03-15

<160> 34    

<170> PatentIn version 3.5

<210> 1
<211> 1317
<212> DNA
<213> Clostridium phytofermentans

<400> 1
atgaagaact atttccccaa cgtcccagaa gtcaaatacg aaggtccaaa ctccacaaat       60

cctttcgctt ttaaatatta tgatgctaat aaagtagtcg ccggtaagac catgaaggag      120

cattgtagat tcgctctatc ctggtggcac actttgtgtg ccggtggtgc tgatccattc      180

ggagtaacta ctatggacag gacctacggt aacattaccg acccaatgga actagctaag      240

gccaaagttg atgctggttt cgaactgatg actaagctgg gcatcgagtt cttctgcttc      300

catgatgccg acattgctcc agaaggtgac accttcgaag agtccaagaa gaatctgttc      360

gagattgttg attacatcaa ggagaagatg gaccaaaccg gcatcaagtt gttatggggc      420

actgctaaca actttagtca ccccaggttc atgcacggtg catcaacttc ttgtaatgcc      480

gatgttttcg cttatgctgc tgcgaaaata aagaacgctt tagatgcgac catcaagttg      540

ggcggtaagg gttatgtctt ttggggtggt agagaaggtt acgagaccct gctgaatact      600

gacctgggct tagaactgga caacatggct aggctaatga agatggccgt agaatacggt      660

agggctaatg gattcgacgg tgacttctac atcgagccta aacccaagga acctactaag      720

caccagtacg acttcgacac tgctaccgta ttagcttttt taaggaagta cgggttggaa      780

aaagacttca agatgaacat cgaagccaat cacgccacac tagcaggcca cacattcgag      840

catgagttag ctatggctag ggtaaacggt gcattcggtt ctgttgatgc taaccaaggt      900

gacccaaact taggatggga cacggatcaa ttccccacag acgttcattc tgctactctt      960

gctatgctgg aggtcttgaa agccggtggt ttcacaaatg gcggcctgaa ctttgatgcg     1020

aaagttcgta ggggttcatt cgagtttgac gatattgcct atggttacat tgctggtatg     1080

gatactttcg cgttagggtt aattaaagct gctgaaatca ttgatgacgg tagaattgcc     1140

aagtttgtgg atgacaggta tgcctcttac aagaccggta ttggtaaagc gatcgttgac     1200

ggaactacct ctttggaaga attggaacaa tacgtgttga ctcattctga acctgtcatg     1260

caatctggta gacaagaggt tctggaaact attgtcaaca acatattgtt tagataa        1317


<210> 2
<211> 438
<212> PRT
<213> Clostridium phytofermentans

<400> 2
Met Lys Asn Tyr Phe Pro Asn Val Pro Glu Val Lys Tyr Glu Gly Pro 
1               5                   10                  15      


Asn Ser Thr Asn Pro Phe Ala Phe Lys Tyr Tyr Asp Ala Asn Lys Val 
            20                  25                  30          


Val Ala Gly Lys Thr Met Lys Glu His Cys Arg Phe Ala Leu Ser Trp 
        35                  40                  45              


Trp His Thr Leu Cys Ala Gly Gly Ala Asp Pro Phe Gly Val Thr Thr 
    50                  55                  60                  


Met Asp Arg Thr Tyr Gly Asn Ile Thr Asp Pro Met Glu Leu Ala Lys 
65                  70                  75                  80  


Ala Lys Val Asp Ala Gly Phe Glu Leu Met Thr Lys Leu Gly Ile Glu 
                85                  90                  95      


Phe Phe Cys Phe His Asp Ala Asp Ile Ala Pro Glu Gly Asp Thr Phe 
            100                 105                 110         


Glu Glu Ser Lys Lys Asn Leu Phe Glu Ile Val Asp Tyr Ile Lys Glu 
        115                 120                 125             


Lys Met Asp Gln Thr Gly Ile Lys Leu Leu Trp Gly Thr Ala Asn Asn 
    130                 135                 140                 


Phe Ser His Pro Arg Phe Met His Gly Ala Ser Thr Ser Cys Asn Ala 
145                 150                 155                 160 


Asp Val Phe Ala Tyr Ala Ala Ala Lys Ile Lys Asn Ala Leu Asp Ala 
                165                 170                 175     


Thr Ile Lys Leu Gly Gly Lys Gly Tyr Val Phe Trp Gly Gly Arg Glu 
            180                 185                 190         


Gly Tyr Glu Thr Leu Leu Asn Thr Asp Leu Gly Leu Glu Leu Asp Asn 
        195                 200                 205             


Met Ala Arg Leu Met Lys Met Ala Val Glu Tyr Gly Arg Ala Asn Gly 
    210                 215                 220                 


Phe Asp Gly Asp Phe Tyr Ile Glu Pro Lys Pro Lys Glu Pro Thr Lys 
225                 230                 235                 240 


His Gln Tyr Asp Phe Asp Thr Ala Thr Val Leu Ala Phe Leu Arg Lys 
                245                 250                 255     


Tyr Gly Leu Glu Lys Asp Phe Lys Met Asn Ile Glu Ala Asn His Ala 
            260                 265                 270         


Thr Leu Ala Gly His Thr Phe Glu His Glu Leu Ala Met Ala Arg Val 
        275                 280                 285             


Asn Gly Ala Phe Gly Ser Val Asp Ala Asn Gln Gly Asp Pro Asn Leu 
    290                 295                 300                 


Gly Trp Asp Thr Asp Gln Phe Pro Thr Asp Val His Ser Ala Thr Leu 
305                 310                 315                 320 


Ala Met Leu Glu Val Leu Lys Ala Gly Gly Phe Thr Asn Gly Gly Leu 
                325                 330                 335     


Asn Phe Asp Ala Lys Val Arg Arg Gly Ser Phe Glu Phe Asp Asp Ile 
            340                 345                 350         


Ala Tyr Gly Tyr Ile Ala Gly Met Asp Thr Phe Ala Leu Gly Leu Ile 
        355                 360                 365             


Lys Ala Ala Glu Ile Ile Asp Asp Gly Arg Ile Ala Lys Phe Val Asp 
    370                 375                 380                 


Asp Arg Tyr Ala Ser Tyr Lys Thr Gly Ile Gly Lys Ala Ile Val Asp 
385                 390                 395                 400 


Gly Thr Thr Ser Leu Glu Glu Leu Glu Gln Tyr Val Leu Thr His Ser 
                405                 410                 415     


Glu Pro Val Met Gln Ser Gly Arg Gln Glu Val Leu Glu Thr Ile Val 
            420                 425                 430         


Asn Asn Ile Leu Phe Arg 
        435             


<210> 3
<211> 1326
<212> DNA
<213> Abiotrophia defective

<400> 3
atgagtgaat tgttccaaaa catcccaaaa atcaaatacg aaggtgcaaa ttccaaaaat       60

cctttggctt ttcattatta tgatgctgaa aaaatagtcc tcggtaagac catgaaggag      120

catttgccat tcgctatggc atggtggcac aatttgtgtg ccgctggtac tgatatgttc      180

ggacgtgata ctgcggacaa gtcctttggt ttggaaaaag gctcaatgga acatgctaag      240

gccaaagttg atgctggttt cgaatttatg gaaaagctgg gcattaaata cttctgcttc      300

catgatgtag accttgttcc agaagcttgc gacattaaag agaccaattc tcgactggac      360

gaaatttctg attacatctt ggagaagatg aagggcactg atattaagtg tttatggggc      420

actgctaata tgttttctaa ccccaggttc gtgaacggtg caggatctac taatagtgcc      480

gatgtttact gttttgctgc tgcgcaaata aagaaagcat tagatattac cgtcaagttg      540

ggcggtagag gttatgtctt ttggggtggt agagaaggtt acgagaccct gctgaatact      600

gacgtgaaat ttgaacagga aaacattgct aatctaatga agatggccgt agaatacggt      660

aggtctattg gattcaaagg tgacttctac atcgagccta aacccaagga acctatgaag      720

caccagtacg acttcgacgc tgctaccgca ataggttttt taaggcagta cgggttggat      780

aaagacttca aattgaacat cgaagccaat cacgccacac tagcaggaca ctcattccag      840

catgagttac gtatttctag tattaacggt atgttgggtt ctgttgatgc taaccaaggt      900

gacatgttgt taggatggga cacggatgaa tttccctttg acgtttatga tactactatg      960

tgtatgtatg aggtccttaa aaacggtggt ttgacaggcg gctttaactt tgatgcgaaa     1020

aatcgtaggc cttcatacac gtatgaagat atgttctatg gtttcattct tggtatggat     1080

tctttcgcgt tagggttgat aaaagctgct aaattgattg aagaaggtac acttgacaat     1140

tttattaagg aaaggtataa atcttttgaa tccgaaattg gtaaaaaaat tagatccaaa     1200

tcagcctctt tgcaagaatt ggcagcttat gctgaggaaa tgggtgctcc cgcgatgccg     1260

ggttcaggta ggcaagagta tctgcaagct gctctcaacc aaaatttgtt tggtgaagtg     1320

taataa                                                                1326


<210> 4
<211> 440
<212> PRT
<213> Abiotrophia defective

<400> 4
Met Ser Glu Leu Phe Gln Asn Ile Pro Lys Ile Lys Tyr Glu Gly Ala 
1               5                   10                  15      


Asn Ser Lys Asn Pro Leu Ala Phe His Tyr Tyr Asp Ala Glu Lys Ile 
            20                  25                  30          


Val Leu Gly Lys Thr Met Lys Glu His Leu Pro Phe Ala Met Ala Trp 
        35                  40                  45              


Trp His Asn Leu Cys Ala Ala Gly Thr Asp Met Phe Gly Arg Asp Thr 
    50                  55                  60                  


Ala Asp Lys Ser Phe Gly Leu Glu Lys Gly Ser Met Glu His Ala Lys 
65                  70                  75                  80  


Ala Lys Val Asp Ala Gly Phe Glu Phe Met Glu Lys Leu Gly Ile Lys 
                85                  90                  95      


Tyr Phe Cys Phe His Asp Val Asp Leu Val Pro Glu Ala Cys Asp Ile 
            100                 105                 110         


Lys Glu Thr Asn Ser Arg Leu Asp Glu Ile Ser Asp Tyr Ile Leu Glu 
        115                 120                 125             


Lys Met Lys Gly Thr Asp Ile Lys Cys Leu Trp Gly Thr Ala Asn Met 
    130                 135                 140                 


Phe Ser Asn Pro Arg Phe Val Asn Gly Ala Gly Ser Thr Asn Ser Ala 
145                 150                 155                 160 


Asp Val Tyr Cys Phe Ala Ala Ala Gln Ile Lys Lys Ala Leu Asp Ile 
                165                 170                 175     


Thr Val Lys Leu Gly Gly Arg Gly Tyr Val Phe Trp Gly Gly Arg Glu 
            180                 185                 190         


Gly Tyr Glu Thr Leu Leu Asn Thr Asp Val Lys Phe Glu Gln Glu Asn 
        195                 200                 205             


Ile Ala Asn Leu Met Lys Met Ala Val Glu Tyr Gly Arg Ser Ile Gly 
    210                 215                 220                 


Phe Lys Gly Asp Phe Tyr Ile Glu Pro Lys Pro Lys Glu Pro Met Lys 
225                 230                 235                 240 


His Gln Tyr Asp Phe Asp Ala Ala Thr Ala Ile Gly Phe Leu Arg Gln 
                245                 250                 255     


Tyr Gly Leu Asp Lys Asp Phe Lys Leu Asn Ile Glu Ala Asn His Ala 
            260                 265                 270         


Thr Leu Ala Gly His Ser Phe Gln His Glu Leu Arg Ile Ser Ser Ile 
        275                 280                 285             


Asn Gly Met Leu Gly Ser Val Asp Ala Asn Gln Gly Asp Met Leu Leu 
    290                 295                 300                 


Gly Trp Asp Thr Asp Glu Phe Pro Phe Asp Val Tyr Asp Thr Thr Met 
305                 310                 315                 320 


Cys Met Tyr Glu Val Leu Lys Asn Gly Gly Leu Thr Gly Gly Phe Asn 
                325                 330                 335     


Phe Asp Ala Lys Asn Arg Arg Pro Ser Tyr Thr Tyr Glu Asp Met Phe 
            340                 345                 350         


Tyr Gly Phe Ile Leu Gly Met Asp Ser Phe Ala Leu Gly Leu Ile Lys 
        355                 360                 365             


Ala Ala Lys Leu Ile Glu Glu Gly Thr Leu Asp Asn Phe Ile Lys Glu 
    370                 375                 380                 


Arg Tyr Lys Ser Phe Glu Ser Glu Ile Gly Lys Lys Ile Arg Ser Lys 
385                 390                 395                 400 


Ser Ala Ser Leu Gln Glu Leu Ala Ala Tyr Ala Glu Glu Met Gly Ala 
                405                 410                 415     


Pro Ala Met Pro Gly Ser Gly Arg Gln Glu Tyr Leu Gln Ala Ala Leu 
            420                 425                 430         


Asn Gln Asn Leu Phe Gly Glu Val 
        435                 440 


<210> 5
<211> 1317
<212> DNA
<213> Ruminococcus flavifaciens

<400> 5
atggaatttt tctccaacat cggaaaaatc caataccaag gtccaaaatc cacagatcct       60

ttgtctttta aatattataa tcctgaagaa gtaatcaacg gtaagaccat gagggagcat      120

ttgaaattcg ctctatcctg gtggcacact atgggtggcg atggtactga tatgttcgga      180

tgtggtacta cggacaagac ctggggtcaa tccgacccag cggcaagagc taaggccaaa      240

gttgatgctg ctttcgaaat tatggataag ctgagcattg attactactg cttccatgat      300

agagaccttt ctccagaata tggctccttg aaagcgacca atgatcaact ggacattgtt      360

actgattaca tcaaggagaa gcagggcgat aaattcaagt gtttatgggg cactgctaaa      420

tgctttgatc accccaggtt catgcacggt gcaggaactt ctcctagtgc cgatgttttc      480

gctttttctg ctgcgcaaat aaagaaagca ttagaatcta ccgtcaagtt gggcggtaat      540

ggttatgtct tttggggtgg tagagaaggt tacgagaccc tgctgaatac taacatgggc      600

ttagaactgg acaacatggc taggctaatg aagatggccg tagaatacgg taggtctatt      660

ggattcaaag gtgacttcta catcgagcct aaacccaagg aacctactaa gcaccagtac      720

gacttcgaca ctgctaccgt attaggtttt ttaaggaagt acgggttgga taaagacttc      780

aagatgaaca tcgaagccaa tcacgccaca ctagcacaac acacattcca gcatgagtta      840

cgtgtggcta gggataacgg tgtattcggt tctattgatg ctaaccaagg tgacgtattg      900

ttaggatggg acacggatca attccccaca aacatttatg atactactat gtgtatgtat      960

gaggtcatta aagccggtgg tttcacaaat ggcggcctga actttgatgc gaaagctcgt     1020

aggggttcat tcacgcctga agatattttc tatagttaca ttgctggtat ggatgctttc     1080

gcgttagggt ttagagcagc tcttaaattg attgaagacg gtagaattga caagtttgtg     1140

gctgacaggt atgcctcttg gaataccggt attggtgcag atattattgc cggaaaagcc     1200

gattttgcat cattggaaaa atatgctttg gaaaaaggtg aagttaccgc gtcattgtct     1260

tctggtagac aagagatgct ggaatctatt gtcaacaacg tattgtttag tttgtaa        1317


<210> 6
<211> 438
<212> PRT
<213> Ruminococcus flavifaciens

<400> 6
Met Glu Phe Phe Ser Asn Ile Gly Lys Ile Gln Tyr Gln Gly Pro Lys 
1               5                   10                  15      


Ser Thr Asp Pro Leu Ser Phe Lys Tyr Tyr Asn Pro Glu Glu Val Ile 
            20                  25                  30          


Asn Gly Lys Thr Met Arg Glu His Leu Lys Phe Ala Leu Ser Trp Trp 
        35                  40                  45              


His Thr Met Gly Gly Asp Gly Thr Asp Met Phe Gly Cys Gly Thr Thr 
    50                  55                  60                  


Asp Lys Thr Trp Gly Gln Ser Asp Pro Ala Ala Arg Ala Lys Ala Lys 
65                  70                  75                  80  


Val Asp Ala Ala Phe Glu Ile Met Asp Lys Leu Ser Ile Asp Tyr Tyr 
                85                  90                  95      


Cys Phe His Asp Arg Asp Leu Ser Pro Glu Tyr Gly Ser Leu Lys Ala 
            100                 105                 110         


Thr Asn Asp Gln Leu Asp Ile Val Thr Asp Tyr Ile Lys Glu Lys Gln 
        115                 120                 125             


Gly Asp Lys Phe Lys Cys Leu Trp Gly Thr Ala Lys Cys Phe Asp His 
    130                 135                 140                 


Pro Arg Phe Met His Gly Ala Gly Thr Ser Pro Ser Ala Asp Val Phe 
145                 150                 155                 160 


Ala Phe Ser Ala Ala Gln Ile Lys Lys Ala Leu Glu Ser Thr Val Lys 
                165                 170                 175     


Leu Gly Gly Asn Gly Tyr Val Phe Trp Gly Gly Arg Glu Gly Tyr Glu 
            180                 185                 190         


Thr Leu Leu Asn Thr Asn Met Gly Leu Glu Leu Asp Asn Met Ala Arg 
        195                 200                 205             


Leu Met Lys Met Ala Val Glu Tyr Gly Arg Ser Ile Gly Phe Lys Gly 
    210                 215                 220                 


Asp Phe Tyr Ile Glu Pro Lys Pro Lys Glu Pro Thr Lys His Gln Tyr 
225                 230                 235                 240 


Asp Phe Asp Thr Ala Thr Val Leu Gly Phe Leu Arg Lys Tyr Gly Leu 
                245                 250                 255     


Asp Lys Asp Phe Lys Met Asn Ile Glu Ala Asn His Ala Thr Leu Ala 
            260                 265                 270         


Gln His Thr Phe Gln His Glu Leu Arg Val Ala Arg Asp Asn Gly Val 
        275                 280                 285             


Phe Gly Ser Ile Asp Ala Asn Gln Gly Asp Val Leu Leu Gly Trp Asp 
    290                 295                 300                 


Thr Asp Gln Phe Pro Thr Asn Ile Tyr Asp Thr Thr Met Cys Met Tyr 
305                 310                 315                 320 


Glu Val Ile Lys Ala Gly Gly Phe Thr Asn Gly Gly Leu Asn Phe Asp 
                325                 330                 335     


Ala Lys Ala Arg Arg Gly Ser Phe Thr Pro Glu Asp Ile Phe Tyr Ser 
            340                 345                 350         


Tyr Ile Ala Gly Met Asp Ala Phe Ala Leu Gly Phe Arg Ala Ala Leu 
        355                 360                 365             


Lys Leu Ile Glu Asp Gly Arg Ile Asp Lys Phe Val Ala Asp Arg Tyr 
    370                 375                 380                 


Ala Ser Trp Asn Thr Gly Ile Gly Ala Asp Ile Ile Ala Gly Lys Ala 
385                 390                 395                 400 


Asp Phe Ala Ser Leu Glu Lys Tyr Ala Leu Glu Lys Gly Glu Val Thr 
                405                 410                 415     


Ala Ser Leu Ser Ser Gly Arg Gln Glu Met Leu Glu Ser Ile Val Asn 
            420                 425                 430         


Asn Val Leu Phe Ser Leu 
        435             


<210> 7
<211> 1323
<212> DNA
<213> Ruminococcus flavifaciens

<220>
<223> Strain FD-1

<400> 7
atggaatttt tcaagaacat ctctaagata ccatacgaag gcaaagactc taccaatcca       60

ttagcattca agtactacaa tcctgacgaa gtaatcgacg gtaagaagat gagagacatc      120

atgaagtttg ctttgtcttg gtggcatact atgggaggtg atggtactga tatgtttggc      180

tgtggtactg ctgataagac atggggcgag aatgatccag ctgctagagc taaagctaaa      240

gttgatgccg catttgaaat catgcagaag ttatccattg attacttctg cttccatgat      300

agagatttgt ctccagagta cggttctttg aaggacacaa acgctcaatt ggacattgtc      360

actgactaca tcaaggctaa acaagctgaa accggtttga aatgtctttg gggtactgct      420

aagtgcttcg accatccaag attcatgcac ggtgctggta cttctccttc agcggatgtc      480

ttcgcattct cagctgctca aatcaagaaa gctctggaat ctaccgtcaa gttgggtgga      540

actggttatg tcttctgggg tggtagagaa ggatatgaaa cgttgttgaa tactaacatg      600

ggacttgaat tggacaacat ggctaggttg atgaagatgg ccgttgagta tggtaggtct      660

attggtttca aaggtgactt ctacattgaa cctaagccaa aggaaccaac taagcatcaa      720

tacgactttg acactgctac agtcttgggc tttctgagaa agtacggcct ggacaaagac      780

ttcaagatga acatagaagc caatcatgca actttagcgc aacatacctt ccagcacgaa      840

ttgtgtgtcg ccagaactaa tggtgctttc ggttctattg atgctaatca aggtgatccc      900

ttgttgggtt gggatacaga tcagtttcct acaaacatct atgatactac tatgtgcatg      960

tacgaagtta tcaaagctgg tggtttcact aatggtggtc ttaactttga tgctaaagct     1020

agaagaggtt ctttcactcc agaagatatt ttctattctt acattgctgg tatggatgct     1080

ttcgctttag gttacaaagc tgcttctaag ctaatcgctg atggtaggat tgatagcttc     1140

attagcgata gatatgcttc ttggtctgaa ggtattggtt tggacatcat ttccggcaaa     1200

gctgatatgg cggctttaga gaagtatgct ttggagaaag gagaggtcac tgattctatc     1260

tcttctggaa gacaggaact gttagagtcc attgttaaca acgtaatctt caacctataa     1320

taa                                                                   1323


<210> 8
<211> 439
<212> PRT
<213> Ruminococcus flavifaciens

<220>
<223> Strain FD-1

<400> 8
Met Glu Phe Phe Lys Asn Ile Ser Lys Ile Pro Tyr Glu Gly Lys Asp 
1               5                   10                  15      


Ser Thr Asn Pro Leu Ala Phe Lys Tyr Tyr Asn Pro Asp Glu Val Ile 
            20                  25                  30          


Asp Gly Lys Lys Met Arg Asp Ile Met Lys Phe Ala Leu Ser Trp Trp 
        35                  40                  45              


His Thr Met Gly Gly Asp Gly Thr Asp Met Phe Gly Cys Gly Thr Ala 
    50                  55                  60                  


Asp Lys Thr Trp Gly Glu Asn Asp Pro Ala Ala Arg Ala Lys Ala Lys 
65                  70                  75                  80  


Val Asp Ala Ala Phe Glu Ile Met Gln Lys Leu Ser Ile Asp Tyr Phe 
                85                  90                  95      


Cys Phe His Asp Arg Asp Leu Ser Pro Glu Tyr Gly Ser Leu Lys Asp 
            100                 105                 110         


Thr Asn Ala Gln Leu Asp Ile Val Thr Asp Tyr Ile Lys Ala Lys Gln 
        115                 120                 125             


Ala Glu Thr Gly Leu Lys Cys Leu Trp Gly Thr Ala Lys Cys Phe Asp 
    130                 135                 140                 


His Pro Arg Phe Met His Gly Ala Gly Thr Ser Pro Ser Ala Asp Val 
145                 150                 155                 160 


Phe Ala Phe Ser Ala Ala Gln Ile Lys Lys Ala Leu Glu Ser Thr Val 
                165                 170                 175     


Lys Leu Gly Gly Thr Gly Tyr Val Phe Trp Gly Gly Arg Glu Gly Tyr 
            180                 185                 190         


Glu Thr Leu Leu Asn Thr Asn Met Gly Leu Glu Leu Asp Asn Met Ala 
        195                 200                 205             


Arg Leu Met Lys Met Ala Val Glu Tyr Gly Arg Ser Ile Gly Phe Lys 
    210                 215                 220                 


Gly Asp Phe Tyr Ile Glu Pro Lys Pro Lys Glu Pro Thr Lys His Gln 
225                 230                 235                 240 


Tyr Asp Phe Asp Thr Ala Thr Val Leu Gly Phe Leu Arg Lys Tyr Gly 
                245                 250                 255     


Leu Asp Lys Asp Phe Lys Met Asn Ile Glu Ala Asn His Ala Thr Leu 
            260                 265                 270         


Ala Gln His Thr Phe Gln His Glu Leu Cys Val Ala Arg Thr Asn Gly 
        275                 280                 285             


Ala Phe Gly Ser Ile Asp Ala Asn Gln Gly Asp Pro Leu Leu Gly Trp 
    290                 295                 300                 


Asp Thr Asp Gln Phe Pro Thr Asn Ile Tyr Asp Thr Thr Met Cys Met 
305                 310                 315                 320 


Tyr Glu Val Ile Lys Ala Gly Gly Phe Thr Asn Gly Gly Leu Asn Phe 
                325                 330                 335     


Asp Ala Lys Ala Arg Arg Gly Ser Phe Thr Pro Glu Asp Ile Phe Tyr 
            340                 345                 350         


Ser Tyr Ile Ala Gly Met Asp Ala Phe Ala Leu Gly Tyr Lys Ala Ala 
        355                 360                 365             


Ser Lys Leu Ile Ala Asp Gly Arg Ile Asp Ser Phe Ile Ser Asp Arg 
    370                 375                 380                 


Tyr Ala Ser Trp Ser Glu Gly Ile Gly Leu Asp Ile Ile Ser Gly Lys 
385                 390                 395                 400 


Ala Asp Met Ala Ala Leu Glu Lys Tyr Ala Leu Glu Lys Gly Glu Val 
                405                 410                 415     


Thr Asp Ser Ile Ser Ser Gly Arg Gln Glu Leu Leu Glu Ser Ile Val 
            420                 425                 430         


Asn Asn Val Ile Phe Asn Leu 
        435                 


<210> 9
<211> 1410
<212> DNA
<213> Phytopthara infestans

<400> 9
atgcaacatc aagtgaaaga atatttccca aacgtcccaa aaatcacatt cgaaggtcaa       60

aatgccaaaa gtgttttggc ttatcgtgaa tataatgctt cagaagtaat catgggtaag      120

accatggagg agtggtgtag attcgctgtg tgttattggc acacttttgg taactctggt      180

tctgatccgt tcggaggtga aacttatacc aatagattgt ggaatgaatc attggaaaga      240

gctaatattt cttctaggga aagattgttg gaagctgcta agtgcaaagc tgatgctgct      300

ttcgaaactt ttacaaagct gggcgttaaa tactacacct tccatgatgt agaccttatt      360

tcagaaggtg ccaaccttga agagtcccaa tctctactgg acgaaatttc tgattacttg      420

ttggataagc agaatcaaac tggtgttagg tgtttatggg gcactactaa tttgtttggt      480

cacagaaggt tcatgaacgg tgcatcaact aatcctgata tgaaagtttt cgctcatgct      540

gctgcgagag taaagaaagc aatggaaatt accttgaagt tgggcggtca aaattttgtc      600

ttttggggtg gtagagaagg tttccagtcc attctgaata ctgacatgaa aactgaactg      660

gatcacatgg ctgctttttt taagttggtc gtagcataca aaaaggaact tggagccaca      720

tttcaattct tggtcgagcc taaacccagg gaacctatga agcaccagta cgactacgac      780

gctgctaccg tagtagcttt tttacatacg tacgggttgc aaaatgactt caaattgaac      840

atcgaaccca atcacaccac actagcagga cacgattacg agcatgatat atattatgct      900

gctagttaca aaatgttggg ttctgttgat tgtaacacag gtgacccgtt ggtaggatgg      960

gacacggatc aatttttgat ggacgaaaaa aaagctgttt tggttatgaa aaagatcgtt     1020

gaaatcggtg gtttggcacc aggcggcttg aactttgatg cgaaagttcg tagggaatca     1080

accgatttgg aagatatttt cattgctcac attggtagta tggattgttt cgcgagaggg     1140

ttgagacaag ctgctaaatt gcttgaaaaa aatgaacttg gcgaattggt taagcaaagg     1200

tatgcatctt ggaaatccac acttggtgaa agaattgaac aaggacaagc cactttggaa     1260

gaagtggcag cttatgctaa ggaaagtggt gaacccgatc atgtgtcagg taagcaagag     1320

ttggcggaac ttatgtggag cacagttgcg ttggctacag ggatttggca agatcatgtt     1380

acttgttctt tgactaaaaa ttggtgttaa                                      1410


<210> 10
<211> 469
<212> PRT
<213> Phytopthara infestans

<400> 10
Met Gln His Gln Val Lys Glu Tyr Phe Pro Asn Val Pro Lys Ile Thr 
1               5                   10                  15      


Phe Glu Gly Gln Asn Ala Lys Ser Val Leu Ala Tyr Arg Glu Tyr Asn 
            20                  25                  30          


Ala Ser Glu Val Ile Met Gly Lys Thr Met Glu Glu Trp Cys Arg Phe 
        35                  40                  45              


Ala Val Cys Tyr Trp His Thr Phe Gly Asn Ser Gly Ser Asp Pro Phe 
    50                  55                  60                  


Gly Gly Glu Thr Tyr Thr Asn Arg Leu Trp Asn Glu Ser Leu Glu Arg 
65                  70                  75                  80  


Ala Asn Ile Ser Ser Arg Glu Arg Leu Leu Glu Ala Ala Lys Cys Lys 
                85                  90                  95      


Ala Asp Ala Ala Phe Glu Thr Phe Thr Lys Leu Gly Val Lys Tyr Tyr 
            100                 105                 110         


Thr Phe His Asp Val Asp Leu Ile Ser Glu Gly Ala Asn Leu Glu Glu 
        115                 120                 125             


Ser Gln Ser Leu Leu Asp Glu Ile Ser Asp Tyr Leu Leu Asp Lys Gln 
    130                 135                 140                 


Asn Gln Thr Gly Val Arg Cys Leu Trp Gly Thr Thr Asn Leu Phe Gly 
145                 150                 155                 160 


His Arg Arg Phe Met Asn Gly Ala Ser Thr Asn Pro Asp Met Lys Val 
                165                 170                 175     


Phe Ala His Ala Ala Ala Arg Val Lys Lys Ala Met Glu Ile Thr Leu 
            180                 185                 190         


Lys Leu Gly Gly Gln Asn Phe Val Phe Trp Gly Gly Arg Glu Gly Phe 
        195                 200                 205             


Gln Ser Ile Leu Asn Thr Asp Met Lys Thr Glu Leu Asp His Met Ala 
    210                 215                 220                 


Ala Phe Phe Lys Leu Val Val Ala Tyr Lys Lys Glu Leu Gly Ala Thr 
225                 230                 235                 240 


Phe Gln Phe Leu Val Glu Pro Lys Pro Arg Glu Pro Met Lys His Gln 
                245                 250                 255     


Tyr Asp Tyr Asp Ala Ala Thr Val Val Ala Phe Leu His Thr Tyr Gly 
            260                 265                 270         


Leu Gln Asn Asp Phe Lys Leu Asn Ile Glu Pro Asn His Thr Thr Leu 
        275                 280                 285             


Ala Gly His Asp Tyr Glu His Asp Ile Tyr Tyr Ala Ala Ser Tyr Lys 
    290                 295                 300                 


Met Leu Gly Ser Val Asp Cys Asn Thr Gly Asp Pro Leu Val Gly Trp 
305                 310                 315                 320 


Asp Thr Asp Gln Phe Leu Met Asp Glu Lys Lys Ala Val Leu Val Met 
                325                 330                 335     


Lys Lys Ile Val Glu Ile Gly Gly Leu Ala Pro Gly Gly Leu Asn Phe 
            340                 345                 350         


Asp Ala Lys Val Arg Arg Glu Ser Thr Asp Leu Glu Asp Ile Phe Ile 
        355                 360                 365             


Ala His Ile Gly Ser Met Asp Cys Phe Ala Arg Gly Leu Arg Gln Ala 
    370                 375                 380                 


Ala Lys Leu Leu Glu Lys Asn Glu Leu Gly Glu Leu Val Lys Gln Arg 
385                 390                 395                 400 


Tyr Ala Ser Trp Lys Ser Thr Leu Gly Glu Arg Ile Glu Gln Gly Gln 
                405                 410                 415     


Ala Thr Leu Glu Glu Val Ala Ala Tyr Ala Lys Glu Ser Gly Glu Pro 
            420                 425                 430         


Asp His Val Ser Gly Lys Gln Glu Leu Ala Glu Leu Met Trp Ser Thr 
        435                 440                 445             


Val Ala Leu Ala Thr Gly Ile Trp Gln Asp His Val Thr Cys Ser Leu 
    450                 455                 460                 


Thr Lys Asn Trp Cys 
465                 


<210> 11
<211> 54
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 11
gctcattaga aagaaagcat agcaatctaa tctaagtttt ggatcccaaa caaa             54


<210> 12
<211> 54
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 12
acttgataat gaaaactata aatcgtaaag acataagaga tccgccatat gtta             54


<210> 13
<211> 54
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 13
gctcattaga aagaaagcat agcaatctaa tctaagtttt ggatcccaaa caaa             54


<210> 14
<211> 54
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 14
acttgataat gaaaactata aatcgtaaag acataagaga tccgccatat gtta             54


<210> 15
<211> 26
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 15
acggtcttca atttctcaag tttcag                                            26


<210> 16
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 16
tcgtactggt gcttagtagg ttccttgg                                          28


<210> 17
<211> 26
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 17
acggtcttca atttctcaag tttcag                                            26


<210> 18
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 18
gcgaaagaat ccataccaag aatg                                              24


<210> 19
<211> 27
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 19
ttatgtcttt tggggtggta gagaagg                                           27


<210> 20
<211> 25
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 20
tattcgtgaa acttcgaaca ctgtc                                             25


<210> 21
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 21
tgtcttctgg ggtggtagag aagg                                              24


<210> 22
<211> 25
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 22
tattcgtgaa acttcgaaca ctgtc                                             25


<210> 23
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 23
tgtcttttgg ggtggtagag aagg                                              24


<210> 24
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 24
gcgaaacaat ccatactacc aatg                                              24


<210> 25
<211> 27
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 25
ttatgtcttt tggggtggta gagaagg                                           27


<210> 26
<211> 25
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 26
tattcgtgaa acttcgaaca ctgtc                                             25


<210> 27
<211> 27
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 27
ttatgtcttt tggggtggta gagaagg                                           27


<210> 28
<211> 25
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 28
tattcgtgaa acttcgaaca ctgtc                                             25


<210> 29
<211> 26
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 29
acggtcttca atttctcaag tttcag                                            26


<210> 30
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 30
gcgaaagcat ccataccagc aatg                                              24


<210> 31
<211> 26
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 31
acggtcttca atttctcaag tttcag                                            26


<210> 32
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 32
gcgaaagcat ccataccagc aatg                                              24


<210> 33
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 33
tgtcttttgg ggtggtagag aagg                                              24


<210> 34
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 34
gcgaaacaat ccatactacc aatg                                              24


