                         SEQUENCE LISTING

<110>  Crucell Holland B.V.
       Radosevic, Katarina
       Widjojoatmodjo, Myra
       Custers, Jerome
       Vellinga, Jort
 
<120>  Vaccine against RSV

<130>  0200 EP P00 PRI

<160>  2     

<170>  PatentIn version 3.3

<210>  1
<211>  574
<212>  PRT
<213>  respiratory syncytial virus


<220>
<221>  RSV F protein (A2 strain)
<222>  (1)..(574)

<400>  1

Met Glu Leu Leu Ile Leu Lys Ala Asn Ala Ile Thr Thr Ile Leu Thr 
1               5                   10                  15      


Ala Val Thr Phe Cys Phe Ala Ser Gly Gln Asn Ile Thr Glu Glu Phe 
            20                  25                  30          


Tyr Gln Ser Thr Cys Ser Ala Val Ser Lys Gly Tyr Leu Ser Ala Leu 
        35                  40                  45              


Arg Thr Gly Trp Tyr Thr Ser Val Ile Thr Ile Glu Leu Ser Asn Ile 
    50                  55                  60                  


Lys Lys Asn Lys Cys Asn Gly Thr Asp Ala Lys Ile Lys Leu Ile Lys 
65                  70                  75                  80  


Gln Glu Leu Asp Lys Tyr Lys Asn Ala Val Thr Glu Leu Gln Leu Leu 
                85                  90                  95      


Met Gln Ser Thr Pro Ala Thr Asn Asn Arg Ala Arg Arg Glu Leu Pro 
            100                 105                 110         


Arg Phe Met Asn Tyr Thr Leu Asn Asn Ala Lys Lys Thr Asn Val Thr 
        115                 120                 125             


Leu Ser Lys Lys Arg Lys Arg Arg Phe Leu Gly Phe Leu Leu Gly Val 
    130                 135                 140                 


Gly Ser Ala Ile Ala Ser Gly Val Ala Val Ser Lys Val Leu His Leu 
145                 150                 155                 160 


Glu Gly Glu Val Asn Lys Ile Lys Ser Ala Leu Leu Ser Thr Asn Lys 
                165                 170                 175     


Ala Val Val Ser Leu Ser Asn Gly Val Ser Val Leu Thr Ser Lys Val 
            180                 185                 190         


Leu Asp Leu Lys Asn Tyr Ile Asp Lys Gln Leu Leu Pro Ile Val Asn 
        195                 200                 205             


Lys Gln Ser Cys Ser Ile Ser Asn Ile Glu Thr Val Ile Glu Phe Gln 
    210                 215                 220                 


Gln Lys Asn Asn Arg Leu Leu Glu Ile Thr Arg Glu Phe Ser Val Asn 
225                 230                 235                 240 


Ala Gly Val Thr Thr Pro Val Ser Thr Tyr Met Leu Thr Asn Ser Glu 
                245                 250                 255     


Leu Leu Ser Leu Ile Asn Asp Met Pro Ile Thr Asn Asp Gln Lys Lys 
            260                 265                 270         


Leu Met Ser Asn Asn Val Gln Ile Val Arg Gln Gln Ser Tyr Ser Ile 
        275                 280                 285             


Met Ser Ile Ile Lys Glu Glu Val Leu Ala Tyr Val Val Gln Leu Pro 
    290                 295                 300                 


Leu Tyr Gly Val Ile Asp Thr Pro Cys Trp Lys Leu His Thr Ser Pro 
305                 310                 315                 320 


Leu Cys Thr Thr Asn Thr Lys Glu Gly Ser Asn Ile Cys Leu Thr Arg 
                325                 330                 335     


Thr Asp Arg Gly Trp Tyr Cys Asp Asn Ala Gly Ser Val Ser Phe Phe 
            340                 345                 350         


Pro Gln Ala Glu Thr Cys Lys Val Gln Ser Asn Arg Val Phe Cys Asp 
        355                 360                 365             


Thr Met Asn Ser Leu Thr Leu Pro Ser Glu Val Asn Leu Cys Asn Val 
    370                 375                 380                 


Asp Ile Phe Asn Pro Lys Tyr Asp Cys Lys Ile Met Thr Ser Lys Thr 
385                 390                 395                 400 


Asp Val Ser Ser Ser Val Ile Thr Ser Leu Gly Ala Ile Val Ser Cys 
                405                 410                 415     


Tyr Gly Lys Thr Lys Cys Thr Ala Ser Asn Lys Asn Arg Gly Ile Ile 
            420                 425                 430         


Lys Thr Phe Ser Asn Gly Cys Asp Tyr Val Ser Asn Lys Gly Val Asp 
        435                 440                 445             


Thr Val Ser Val Gly Asn Thr Leu Tyr Tyr Val Asn Lys Gln Glu Gly 
    450                 455                 460                 


Lys Ser Leu Tyr Val Lys Gly Glu Pro Ile Ile Asn Phe Tyr Asp Pro 
465                 470                 475                 480 


Leu Val Phe Pro Ser Asp Glu Phe Asp Ala Ser Ile Ser Gln Val Asn 
                485                 490                 495     


Glu Lys Ile Asn Gln Ser Leu Ala Phe Ile Arg Lys Ser Asp Glu Leu 
            500                 505                 510         


Leu His Asn Val Asn Ala Val Lys Ser Thr Thr Asn Ile Met Ile Thr 
        515                 520                 525             


Thr Ile Ile Ile Val Ile Ile Val Ile Leu Leu Ser Leu Ile Ala Val 
    530                 535                 540                 


Gly Leu Leu Leu Tyr Cys Lys Ala Arg Ser Thr Pro Val Thr Leu Ser 
545                 550                 555                 560 


Lys Asp Gln Leu Ser Gly Ile Asn Asn Ile Ala Phe Ser Asn 
                565                 570                 


<210>  2
<211>  1722
<212>  DNA
<213>  respiratory syncytial virus


<220>
<221>  codon optimized sequence encoding RSV F protein (A2 strain)
<222>  (1)..(1722)

<400>  2
atggaactgc tgatcctgaa ggccaacgcc atcaccacca tcctgaccgc cgtgaccttc     60

tgcttcgcca gcggccagaa catcaccgag gaattctacc agagcacctg tagcgccgtg    120

tccaagggct acctgagcgc cctgcggacc ggctggtaca ccagcgtgat caccatcgag    180

ctgagcaaca tcaaaaagaa caagtgcaac ggcaccgacg ccaaaatcaa gctgatcaag    240

caggaactgg acaagtacaa gaacgccgtg accgagctgc agctgctgat gcagagcacc    300

cccgccacca acaaccgggc cagacgggag ctgccccggt tcatgaacta caccctgaac    360

aacgccaaaa agaccaacgt gaccctgagc aagaagcgga agcggcggtt cctgggcttc    420

ctgctgggcg tgggcagcgc cattgctagc ggagtggctg tgtctaaggt gctgcacctg    480

gaaggcgaag tgaacaagat caagtccgcc ctgctgagca ccaacaaggc cgtggtgtcc    540

ctgagcaacg gcgtgtccgt gctgaccagc aaggtgctgg atctgaagaa ctacatcgac    600

aagcagctgc tgcccatcgt gaacaagcag agctgcagca tcagcaacat cgagacagtg    660

atcgagttcc agcagaagaa caaccggctg ctggaaatca cccgcgagtt cagcgtgaac    720

gccggcgtga ccacccccgt gtccacctac atgctgacca acagcgagct gctgagcctg    780

atcaacgaca tgcccatcac caacgaccag aaaaagctga tgagcaacaa cgtgcagatc    840

gtgcggcagc agagctactc catcatgtcc atcatcaaag aagaggtgct ggcctacgtg    900

gtgcagctgc ccctgtacgg cgtgatcgac accccctgct ggaagctgca caccagcccc    960

ctgtgcacca ccaacaccaa agagggcagc aacatctgcc tgacccggac cgaccggggc   1020

tggtactgcg ataatgccgg cagcgtgtca ttctttccac aagccgagac atgcaaggtg   1080

cagagcaacc gggtgttctg cgacaccatg aacagcctga ccctgcccag cgaggtgaac   1140

ctgtgcaacg tggacatctt caaccctaag tacgactgca agatcatgac ctccaagacc   1200

gacgtgtcca gctccgtgat cacctccctg ggcgccatcg tgtcctgcta cggcaagacc   1260

aagtgcaccg ccagcaacaa gaaccggggc atcatcaaga ccttcagcaa cggctgcgac   1320

tacgtgtcca acaagggcgt ggacaccgtg tccgtgggca acaccctgta ctacgtgaac   1380

aaacaggaag gcaagagcct gtacgtgaag ggcgagccca tcatcaactt ctacgacccc   1440

ctggtgttcc ccagcgacga gttcgacgcc agcatcagcc aggtcaacga gaagatcaac   1500

cagagcctgg ccttcatcag aaagagcgac gagctgctgc acaatgtgaa tgccgtgaag   1560

tccaccacca atatcatgat caccacaatc atcatcgtga tcatcgtcat cctgctgtcc   1620

ctgatcgccg tgggcctgct gctgtactgc aaggcccggt ccacccctgt gaccctgtcc   1680

aaggaccagc tgagcggcat caacaatatc gccttctcca ac                      1722


