                         SEQUENCE LISTING

<110>  Mascoma Corporation
       Dartmouth College
       Lo, Jonathan
       GUSS, Adam M.
       van DIJKEN, Johannes Pieter
       SHAW, Arthur J. IV
       OLSON, Daniel G.
       HERRING, Christopher D.
 
<120>  ENGINEERINING AN INCREASE IN ETHANOL PRODUCTION BY ALTERING 
       COFACTOR SPECIFICITY

<130>  2608.065PC01

<140>  To Be Assigned
<141>  Herewith

<150>  61/565,261
<151>  2011-11-30

<160>  61    

<170>  PatentIn version 3.5

<210>  1
<211>  1200
<212>  DNA
<213>  Moorella thermoacetica Alcohol dehydrogenase

<400>  1
atgtgggaaa caaaaataaa catcaacgaa gtccgggaaa tccgggctaa aacaaccgtc       60

tactttggag ttggagctat taaaaagatt gacgacatag ccagggaatt taaggaaaag      120

ggatacgata ggatcatcgt aataaccggc aagggggctt ataaagccac cggcgcgtgg      180

gaatatatag ttccggcctt aaataaaaac cagataacct atatccatta cgaccaggtg      240

acgcccaacc cgacggtaga ccaggttgac gaggcaacca aacaagcccg ggaattcggt      300

gcccgagccg tcctggccat cggcgggggt agccccattg atgccgctaa aagcgtagcc      360

gtcttgctct cctaccccga caaaaatgcc cgacagctct accagttaga atttacacct      420

gttaaggccg cacctatcat cgctattaat cttacccatg gtacggggac ggaagccgat      480

cgctttgccg ttgtcagcat ccctgaaaag gcatataaac ccgctattgc ctatgattgc      540

atttacccct tatattcaat tgacgacccg gccctcatgg taaaactgcc gtccgaccag      600

acagcttatg tctctgttga tgccctcaac catgtcgtcg aagcagccac cagcaaagta      660

gccagcccct atactattat cctggccaag gaaacggtac ggctcatcgc ccgatacctg      720

ccccaggccc tgtcccatcc ggcggatttg acggccaggt attatctcct ctatgcttcc      780

ctgattgccg gaatagcctt tgacaacggt ttgctccact tcacccacgc cctggaacac      840

cccctgagcg ccgtcaaacc ggagctcgcc cacggtctgg ggctgggtat gctgctgccg      900

gccgtagtca agcagattta cccggcaacc ccggaggtac tggcggagat actggagccc      960

attgttcccg atctcaaagg cgttcccggt gaagcagaaa aggctgccag cggggtggca     1020

aaatggcttg ccggagccgg tattaccatg aagctaaaag atgcgggctt tcaagcggaa     1080

gatatcgcca ggttaactga cctggccttt accaccccga gtctcgagct tctcctgagt     1140

atggccccgg taacggccga cagggaaagg gttaaggcaa tttaccagga cgccttttaa     1200


<210>  2
<211>  399
<212>  PRT
<213>  Moorella thermoacetica Alcohol dehydrogenase

<400>  2

Met Trp Glu Thr Lys Ile Asn Ile Asn Glu Val Arg Glu Ile Arg Ala 
1               5                   10                  15      


Lys Thr Thr Val Tyr Phe Gly Val Gly Ala Ile Lys Lys Ile Asp Asp 
            20                  25                  30          


Ile Ala Arg Glu Phe Lys Glu Lys Gly Tyr Asp Arg Ile Ile Val Ile 
        35                  40                  45              


Thr Gly Lys Gly Ala Tyr Lys Ala Thr Gly Ala Trp Glu Tyr Ile Val 
    50                  55                  60                  


Pro Ala Leu Asn Lys Asn Gln Ile Thr Tyr Ile His Tyr Asp Gln Val 
65                  70                  75                  80  


Thr Pro Asn Pro Thr Val Asp Gln Val Asp Glu Ala Thr Lys Gln Ala 
                85                  90                  95      


Arg Glu Phe Gly Ala Arg Ala Val Leu Ala Ile Gly Gly Gly Ser Pro 
            100                 105                 110         


Ile Asp Ala Ala Lys Ser Val Ala Val Leu Leu Ser Tyr Pro Asp Lys 
        115                 120                 125             


Asn Ala Arg Gln Leu Tyr Gln Leu Glu Phe Thr Pro Val Lys Ala Ala 
    130                 135                 140                 


Pro Ile Ile Ala Ile Asn Leu Thr His Gly Thr Gly Thr Glu Ala Asp 
145                 150                 155                 160 


Arg Phe Ala Val Val Ser Ile Pro Glu Lys Ala Tyr Lys Pro Ala Ile 
                165                 170                 175     


Ala Tyr Asp Cys Ile Tyr Pro Leu Tyr Ser Ile Asp Asp Pro Ala Leu 
            180                 185                 190         


Met Val Lys Leu Pro Ser Asp Gln Thr Ala Tyr Val Ser Val Asp Ala 
        195                 200                 205             


Leu Asn His Val Val Glu Ala Ala Thr Ser Lys Val Ala Ser Pro Tyr 
    210                 215                 220                 


Thr Ile Ile Leu Ala Lys Glu Thr Val Arg Leu Ile Ala Arg Tyr Leu 
225                 230                 235                 240 


Pro Gln Ala Leu Ser His Pro Ala Asp Leu Thr Ala Arg Tyr Tyr Leu 
                245                 250                 255     


Leu Tyr Ala Ser Leu Ile Ala Gly Ile Ala Phe Asp Asn Gly Leu Leu 
            260                 265                 270         


His Phe Thr His Ala Leu Glu His Pro Leu Ser Ala Val Lys Pro Glu 
        275                 280                 285             


Leu Ala His Gly Leu Gly Leu Gly Met Leu Leu Pro Ala Val Val Lys 
    290                 295                 300                 


Gln Ile Tyr Pro Ala Thr Pro Glu Val Leu Ala Glu Ile Leu Glu Pro 
305                 310                 315                 320 


Ile Val Pro Asp Leu Lys Gly Val Pro Gly Glu Ala Glu Lys Ala Ala 
                325                 330                 335     


Ser Gly Val Ala Lys Trp Leu Ala Gly Ala Gly Ile Thr Met Lys Leu 
            340                 345                 350         


Lys Asp Ala Gly Phe Gln Ala Glu Asp Ile Ala Arg Leu Thr Asp Leu 
        355                 360                 365             


Ala Phe Thr Thr Pro Ser Leu Glu Leu Leu Leu Ser Met Ala Pro Val 
    370                 375                 380                 


Thr Ala Asp Arg Glu Arg Val Lys Ala Ile Tyr Gln Asp Ala Phe 
385                 390                 395                 


<210>  3
<211>  1630
<212>  DNA
<213>  Thermoanaerobacter ethanolicus secondary-alcohol dehydrogenase

<400>  3
tgaacaatag acaacccctt tctgtgatct tgttttttgc aaatgctatt ttatcacaag       60

agatttctct agttcttttt tacttaaaaa aaccctacga aattttaaac tatgtcgaat      120

aaattattga taatttttaa ctatgtgcta ttatattatt gcaaaaaatt taacaatcat      180

cgcgtaagct agttttcaca ttaatgactt acccagtatt ttaggaggtg tttaatgatg      240

aaaggttttg caatgctcag tatcggtaaa gttggctgga ttgagaagga aaagcctgct      300

cctggcccat ttgatgctat tgtaagacct ctagctgtgg ccccttgcac ttcggacatt      360

cataccgttt ttgaaggagc cattggcgaa agacataaca tgatactcgg tcacgaagct      420

gtaggtgaag tagttgaagt aggtagtgag gtaaaagatt ttaaacctgg tgatcgcgtt      480

gttgtgccag ctattacccc tgattggtgg acctctgaag tacaaagagg atatcaccag      540

cactccggtg gaatgctggc aggctggaaa ttttcgaatg taaaagatgg tgtttttggt      600

gaattttttc atgtgaatga tgctgatatg aatttagcac atctgcctaa agaaattcca      660

ttggaagctg cagttatgat tcccgatatg atgaccactg gttttcacgg agctgaactg      720

gcagatatag aattaggtgc gacggtagca gttttgggta ttggcccagt aggtcttatg      780

gcagtcgctg gtgccaaatt gcgtggagcc ggaagaatta ttgccgtagg cagtagacca      840

gtttgtgtag atgctgcaaa atactatgga gctactgata ttgtaaacta taaagatggt      900

cctatcgaaa gtcagattat gaatctaact gaaggcaaag gtgtcgatgc tgccatcatc      960

gctggaggaa atgctgacat tatggctaca gcagttaaga ttgttaaacc tggtggcacc     1020

atcgctaatg taaattattt tggcgaagga gaggttttgc ctgttcctcg tcttgaatgg     1080

ggttgcggca tggctcataa aactataaaa ggcgggctat gccccggtgg acgtctaaga     1140

atggaaagac tgattgacct tgttttttat aagcctgtcg atccttctaa gctcgtcact     1200

cacgttttcc agggatttga caatattgaa aaagccttta tgttgatgaa agacaaacca     1260

aaagacctaa tcaaacctgt tgtaatatta gcataaaaat ggggacttag tccattttta     1320

tgctaataag gctaaataca ctggtttttt tatatgacac atcggccagt aaactcttgg     1380

taaaaaaata acaaaaaata gttattttct taacattttt acgccattaa cacttgataa     1440

catcatcgaa gaagtaaata aacaactatt aaataaaaga agaaggagga ttatcatgtt     1500

caaaatttta gaaaaaagag aattggcacc ttccatcaag ttgtttgtaa tagaggcacc     1560

actagtagcc aaaaaagcaa ggccaggcca attcgttatg ctaaggataa aagaaggagg     1620

agaaagaatt                                                            1630


<210>  4
<211>  1404
<212>  DNA
<213>  T.saccharolyticum Acetaldehyde dehydrogenase

<400>  4
atgaaagtta aagaggaaga tattgaagcg atcgtcaaaa aagtcttatc ggaatttaat       60

tttgaaaaaa atactaaaag tttcagagat tttggcgtat ttcaagatat gaatgatgct      120

attcgtgctg caaaagatgc ccagaaaaaa ttgagaaata tgtccatgga gtcgagagaa      180

aagattatac agaatataag aaaaaagatt atggagaata aaaaaatact tgcagagatg      240

ggcgtcagtg aaactggcat ggggaaagta gagcacaaaa taataaaaca tgagcttgta      300

gcacttaaga cacctggtac cgaagatata gtgacaacag catggtctgg cgataaggga      360

ctgacattgg ttgaaatggg gccatttggt gtaataggta cgattactcc ttcgacaaat      420

ccaagtgaaa ccgtcctttg caatagcata ggtatgatag ccgcaggtaa ttcagtcgta      480

tttaatccac atccaggtgc ggtaaatgta tctaattacg ctgtcaagtt agtaaatgaa      540

gcggtgatgg aagctggcgg ccctgagaat ttagtcgcat ctgttgaaaa acctacactt      600

gaaactggaa atattatgtt caagagtcct gatgtttcgc tattagtagc gacaggcgga      660

cctggtgtag taacatcggt tctctcatct ggcaaaaggg caataggagc aggagcagga      720

aatccaccag ttgtagttga tgaaacggca gatataaaaa aagctgcgaa agatatagtc      780

gatggtgcta catttgacaa caatttgcct tgtattgctg aaaaggaagt agtttctgta      840

gataaaataa cagatgaact gatttactac atgcaacaga atggctgcta caagattgag      900

gggcgagaaa ttgaaaagct cattgaactt gtattggatc acaaaggtgg caagataaca      960

ttaaacagga aatgggttgg caaagatgct catttaatac taaaagctat aggcatagat     1020

gctgatgaaa gcgtaaggtg cataattttt gaggcggaaa aagacaatcc gttagtggta     1080

gaagagctga tgatgcctat tttaggaata gtaagagcca aaaatgtaga tgaagcgata     1140

atgattgcga cagagttaga acatggcaat aggcattcag cacatatgca ttctaaaaac     1200

gttgataatt taacaaagtt tggaaaaata attgacactg ctatatttgt aaaaaatgct     1260

ccatcgtatg ccgcgttagg atatggtggt gaaggttatt gcacatttac gattgcaagc     1320

agaacaggtg aaggattgac atctgcaagg acttttacta aaagtcgtag atgtgtcttg     1380

gcagatggat tatcaataag atag                                            1404


<210>  5
<211>  467
<212>  PRT
<213>  T.saccharolyticum Acetaldehyde dehydrogenase

<400>  5

Met Lys Val Lys Glu Glu Asp Ile Glu Ala Ile Val Lys Lys Val Leu 
1               5                   10                  15      


Ser Glu Phe Asn Phe Glu Lys Asn Thr Lys Ser Phe Arg Asp Phe Gly 
            20                  25                  30          


Val Phe Gln Asp Met Asn Asp Ala Ile Arg Ala Ala Lys Asp Ala Gln 
        35                  40                  45              


Lys Lys Leu Arg Asn Met Ser Met Glu Ser Arg Glu Lys Ile Ile Gln 
    50                  55                  60                  


Asn Ile Arg Lys Lys Ile Met Glu Asn Lys Lys Ile Leu Ala Glu Met 
65                  70                  75                  80  


Gly Val Ser Glu Thr Gly Met Gly Lys Val Glu His Lys Ile Ile Lys 
                85                  90                  95      


His Glu Leu Val Ala Leu Lys Thr Pro Gly Thr Glu Asp Ile Val Thr 
            100                 105                 110         


Thr Ala Trp Ser Gly Asp Lys Gly Leu Thr Leu Val Glu Met Gly Pro 
        115                 120                 125             


Phe Gly Val Ile Gly Thr Ile Thr Pro Ser Thr Asn Pro Ser Glu Thr 
    130                 135                 140                 


Val Leu Cys Asn Ser Ile Gly Met Ile Ala Ala Gly Asn Ser Val Val 
145                 150                 155                 160 


Phe Asn Pro His Pro Gly Ala Val Asn Val Ser Asn Tyr Ala Val Lys 
                165                 170                 175     


Leu Val Asn Glu Ala Val Met Glu Ala Gly Gly Pro Glu Asn Leu Val 
            180                 185                 190         


Ala Ser Val Glu Lys Pro Thr Leu Glu Thr Gly Asn Ile Met Phe Lys 
        195                 200                 205             


Ser Pro Asp Val Ser Leu Leu Val Ala Thr Gly Gly Pro Gly Val Val 
    210                 215                 220                 


Thr Ser Val Leu Ser Ser Gly Lys Arg Ala Ile Gly Ala Gly Ala Gly 
225                 230                 235                 240 


Asn Pro Pro Val Val Val Asp Glu Thr Ala Asp Ile Lys Lys Ala Ala 
                245                 250                 255     


Lys Asp Ile Val Asp Gly Ala Thr Phe Asp Asn Asn Leu Pro Cys Ile 
            260                 265                 270         


Ala Glu Lys Glu Val Val Ser Val Asp Lys Ile Thr Asp Glu Leu Ile 
        275                 280                 285             


Tyr Tyr Met Gln Gln Asn Gly Cys Tyr Lys Ile Glu Gly Arg Glu Ile 
    290                 295                 300                 


Glu Lys Leu Ile Glu Leu Val Leu Asp His Lys Gly Gly Lys Ile Thr 
305                 310                 315                 320 


Leu Asn Arg Lys Trp Val Gly Lys Asp Ala His Leu Ile Leu Lys Ala 
                325                 330                 335     


Ile Gly Ile Asp Ala Asp Glu Ser Val Arg Cys Ile Ile Phe Glu Ala 
            340                 345                 350         


Glu Lys Asp Asn Pro Leu Val Val Glu Glu Leu Met Met Pro Ile Leu 
        355                 360                 365             


Gly Ile Val Arg Ala Lys Asn Val Asp Glu Ala Ile Met Ile Ala Thr 
    370                 375                 380                 


Glu Leu Glu His Gly Asn Arg His Ser Ala His Met His Ser Lys Asn 
385                 390                 395                 400 


Val Asp Asn Leu Thr Lys Phe Gly Lys Ile Ile Asp Thr Ala Ile Phe 
                405                 410                 415     


Val Lys Asn Ala Pro Ser Tyr Ala Ala Leu Gly Tyr Gly Gly Glu Gly 
            420                 425                 430         


Tyr Cys Thr Phe Thr Ile Ala Ser Arg Thr Gly Glu Gly Leu Thr Ser 
        435                 440                 445             


Ala Arg Thr Phe Thr Lys Ser Arg Arg Cys Val Leu Ala Asp Gly Leu 
    450                 455                 460                 


Ser Ile Arg 
465         


<210>  6
<211>  2580
<212>  DNA
<213>  T.saccharolyticum bifunctional alcohol-acetaldehyde dehydrogenase

<400>  6
atggcaacga caaaaacgga attagacgtt cagaagcaga tagatctact tgtgtcaaga       60

gcacaagagg ctcagaaaaa attcatgtct tacacgcaag agcaaatcga cgcaatagtt      120

aaggcaatgg ctttagcagg cgttgacaaa cacgtagagc tggcaaagat ggcgtacgaa      180

gagacaaaaa tgggtgtata cgaagataag ataacaaaaa atctcttcgc aacagagtac      240

gtgtaccacg acataaaaaa tgaaaagact gtaggaatca taaacgagaa catagaagaa      300

aactacatgg aagtggcaga accgataggc gtaattgccg gtgtcacacc tgtcacaaac      360

ccaacatcta ccacgatgtt taaatgctta atatccataa agacgcgaaa tcctataata      420

ttcagcttcc atccaaaggc aataaagtgc agcatcgcag cagccaaagt gatgtatgaa      480

gctgcactaa aggcaggcgc acctgaagga tgcataggat ggatagaaac gccatcaatt      540

gaggccacac agcttctcat gacacatcca ggcgtatcgc tgatccttgc aacgggcggt      600

gcaggaatgg taaaagcggc atacagctca ggaaaaccgg cattaggcgt aggtcctggc      660

aatgtgccat gctacatcga aaaatcagca aacataaaga gggctgtatc ggatctcata      720

ctaagcaaga catttgacaa tggagtaata tgcgcatcag agcaggccgt aataatagac      780

gaggaaatag cagatgaagt caaaaagctt atgaaagaat acggctgcta cttcttaaac      840

aaagatgaaa taaagaagct tgagaaattt gcaattgatg agcaaagctg cgccatgagc      900

cctgcagtgg taggtcagcc agcggcgaag attgctgaaa tggcaggctt caaagtcccc      960

gaaggcacaa agatattagt ggcagagtac gaaggagtag gtccaaaata tcctctatca     1020

agggagaaac taagcccgat tcttgcttgc tacaccgtca aagactacaa tgaaggaatc     1080

aaaaagtgcg aggaaatgac tgaattcgga ggtttaggcc actctgctgt aatacactct     1140

gaaaatcaaa acgtcataaa tgaatttgca aggcgagtcc gcacaggaag acttatcgta     1200

aattcaccat catcacaggg agcaatagga gatatataca atacaaacac gccatcactt     1260

acattaggct gtggttctat gggaagaaac tcaacgacag acaatgtaag cgtcaagaac     1320

cttttgaata ttaagcgtgt cgtgataagg aaggatagaa tgaaatggtt caagattcca     1380

ccgaagattt actttgaaag cgggtcactc cagtacctgt gcaaagtcaa aagaaaaaaa     1440

gcgtttatcg tcacagatcc attcatggtt aagcttggct tcgtagacaa agtgacatat     1500

caattagaca aagcaaacat cgaatacgaa atattctcag aagtagagcc agatccatct     1560

gttgacacag tcatgaacgg cgtaaaaata atgaattcgt acaatcctga cttaataatc     1620

gctgtaggcg gtggctctgc aatagacgca gcaaagggaa tgtggctttt ctacgaatat     1680

cctgatacag agtttgaaac attgaggctt aaatttgcag acatcagaaa aagggcattt     1740

aagttcccag aacttggcaa aaaagcgcta ttcatcgcaa taccgacaac aagcggcaca     1800

ggctcagaag tgacagcatt tgccgtaata accgacaaaa agagaaacat caagtatcca     1860

ctggcagact acgaacttac acctgacata gccataatag atcctgacct tacaaagact     1920

gtaccgccat ctgtaacagc agacacaggc atggatgtgc tgacacacgc catagaagca     1980

tacgtatcag taatggcatc agactacaca gatgcactgg cggaaaaggc tataaagatc     2040

gtatttgaat acctgccaag ggcttataaa aacggcaatg atgaagaagc ccgcgaaaag     2100

atgcacaatg cttcctgcat ggctggtatg gcattcacaa atgcattctt aggaataaac     2160

cacagcatgg cacacatact gggcggaaag ttccacatac cacacggaag agcaaatgca     2220

atacttctgc cgtatgtaat aaggtacaat gcagaaaaac ctacaaagtt tgtggcattc     2280

ccacaatacg aatatccaaa agcagcagaa agatatgcgg aaatcgccaa attcttagga     2340

ctgcctgctt caactgttga agaaggcgta gaaagcttaa tagaagctat aaagaacctc     2400

atgaaagagc ttaacattcc gcttacactt aaagacgccg gcatcaacaa agaacagttt     2460

gaaaaagaaa tagaggaaat gtcagacatc gccttcaacg atcagtgcac agggacaaac     2520

ccgagaatgc ctctcacaaa agaaattgca gagatctaca gaaaagcata cggtgcatag     2580


<210>  7
<211>  859
<212>  PRT
<213>  T.saccharolyticum bifunctional alcohol-acetaldehyde dehydrogenase

<400>  7

Met Ala Thr Thr Lys Thr Glu Leu Asp Val Gln Lys Gln Ile Asp Leu 
1               5                   10                  15      


Leu Val Ser Arg Ala Gln Glu Ala Gln Lys Lys Phe Met Ser Tyr Thr 
            20                  25                  30          


Gln Glu Gln Ile Asp Ala Ile Val Lys Ala Met Ala Leu Ala Gly Val 
        35                  40                  45              


Asp Lys His Val Glu Leu Ala Lys Met Ala Tyr Glu Glu Thr Lys Met 
    50                  55                  60                  


Gly Val Tyr Glu Asp Lys Ile Thr Lys Asn Leu Phe Ala Thr Glu Tyr 
65                  70                  75                  80  


Val Tyr His Asp Ile Lys Asn Glu Lys Thr Val Gly Ile Ile Asn Glu 
                85                  90                  95      


Asn Ile Glu Glu Asn Tyr Met Glu Val Ala Glu Pro Ile Gly Val Ile 
            100                 105                 110         


Ala Gly Val Thr Pro Val Thr Asn Pro Thr Ser Thr Thr Met Phe Lys 
        115                 120                 125             


Cys Leu Ile Ser Ile Lys Thr Arg Asn Pro Ile Ile Phe Ser Phe His 
    130                 135                 140                 


Pro Lys Ala Ile Lys Cys Ser Ile Ala Ala Ala Lys Val Met Tyr Glu 
145                 150                 155                 160 


Ala Ala Leu Lys Ala Gly Ala Pro Glu Gly Cys Ile Gly Trp Ile Glu 
                165                 170                 175     


Thr Pro Ser Ile Glu Ala Thr Gln Leu Leu Met Thr His Pro Gly Val 
            180                 185                 190         


Ser Leu Ile Leu Ala Thr Gly Gly Ala Gly Met Val Lys Ala Ala Tyr 
        195                 200                 205             


Ser Ser Gly Lys Pro Ala Leu Gly Val Gly Pro Gly Asn Val Pro Cys 
    210                 215                 220                 


Tyr Ile Glu Lys Ser Ala Asn Ile Lys Arg Ala Val Ser Asp Leu Ile 
225                 230                 235                 240 


Leu Ser Lys Thr Phe Asp Asn Gly Val Ile Cys Ala Ser Glu Gln Ala 
                245                 250                 255     


Val Ile Ile Asp Glu Glu Ile Ala Asp Glu Val Lys Lys Leu Met Lys 
            260                 265                 270         


Glu Tyr Gly Cys Tyr Phe Leu Asn Lys Asp Glu Ile Lys Lys Leu Glu 
        275                 280                 285             


Lys Phe Ala Ile Asp Glu Gln Ser Cys Ala Met Ser Pro Ala Val Val 
    290                 295                 300                 


Gly Gln Pro Ala Ala Lys Ile Ala Glu Met Ala Gly Phe Lys Val Pro 
305                 310                 315                 320 


Glu Gly Thr Lys Ile Leu Val Ala Glu Tyr Glu Gly Val Gly Pro Lys 
                325                 330                 335     


Tyr Pro Leu Ser Arg Glu Lys Leu Ser Pro Ile Leu Ala Cys Tyr Thr 
            340                 345                 350         


Val Lys Asp Tyr Asn Glu Gly Ile Lys Lys Cys Glu Glu Met Thr Glu 
        355                 360                 365             


Phe Gly Gly Leu Gly His Ser Ala Val Ile His Ser Glu Asn Gln Asn 
    370                 375                 380                 


Val Ile Asn Glu Phe Ala Arg Arg Val Arg Thr Gly Arg Leu Ile Val 
385                 390                 395                 400 


Asn Ser Pro Ser Ser Gln Gly Ala Ile Gly Asp Ile Tyr Asn Thr Asn 
                405                 410                 415     


Thr Pro Ser Leu Thr Leu Gly Cys Gly Ser Met Gly Arg Asn Ser Thr 
            420                 425                 430         


Thr Asp Asn Val Ser Val Lys Asn Leu Leu Asn Ile Lys Arg Val Val 
        435                 440                 445             


Ile Arg Lys Asp Arg Met Lys Trp Phe Lys Ile Pro Pro Lys Ile Tyr 
    450                 455                 460                 


Phe Glu Ser Gly Ser Leu Gln Tyr Leu Cys Lys Val Lys Arg Lys Lys 
465                 470                 475                 480 


Ala Phe Ile Val Thr Asp Pro Phe Met Val Lys Leu Gly Phe Val Asp 
                485                 490                 495     


Lys Val Thr Tyr Gln Leu Asp Lys Ala Asn Ile Glu Tyr Glu Ile Phe 
            500                 505                 510         


Ser Glu Val Glu Pro Asp Pro Ser Val Asp Thr Val Met Asn Gly Val 
        515                 520                 525             


Lys Ile Met Asn Ser Tyr Asn Pro Asp Leu Ile Ile Ala Val Gly Gly 
    530                 535                 540                 


Gly Ser Ala Ile Asp Ala Ala Lys Gly Met Trp Leu Phe Tyr Glu Tyr 
545                 550                 555                 560 


Pro Asp Thr Glu Phe Glu Thr Leu Arg Leu Lys Phe Ala Asp Ile Arg 
                565                 570                 575     


Lys Arg Ala Phe Lys Phe Pro Glu Leu Gly Lys Lys Ala Leu Phe Ile 
            580                 585                 590         


Ala Ile Pro Thr Thr Ser Gly Thr Gly Ser Glu Val Thr Ala Phe Ala 
        595                 600                 605             


Val Ile Thr Asp Lys Lys Arg Asn Ile Lys Tyr Pro Leu Ala Asp Tyr 
    610                 615                 620                 


Glu Leu Thr Pro Asp Ile Ala Ile Ile Asp Pro Asp Leu Thr Lys Thr 
625                 630                 635                 640 


Val Pro Pro Ser Val Thr Ala Asp Thr Gly Met Asp Val Leu Thr His 
                645                 650                 655     


Ala Ile Glu Ala Tyr Val Ser Val Met Ala Ser Asp Tyr Thr Asp Ala 
            660                 665                 670         


Leu Ala Glu Lys Ala Ile Lys Ile Val Phe Glu Tyr Leu Pro Arg Ala 
        675                 680                 685             


Tyr Lys Asn Gly Asn Asp Glu Glu Ala Arg Glu Lys Met His Asn Ala 
    690                 695                 700                 


Ser Cys Met Ala Gly Met Ala Phe Thr Asn Ala Phe Leu Gly Ile Asn 
705                 710                 715                 720 


His Ser Met Ala His Ile Leu Gly Gly Lys Phe His Ile Pro His Gly 
                725                 730                 735     


Arg Ala Asn Ala Ile Leu Leu Pro Tyr Val Ile Arg Tyr Asn Ala Glu 
            740                 745                 750         


Lys Pro Thr Lys Phe Val Ala Phe Pro Gln Tyr Glu Tyr Pro Lys Ala 
        755                 760                 765             


Ala Glu Arg Tyr Ala Glu Ile Ala Lys Phe Leu Gly Leu Pro Ala Ser 
    770                 775                 780                 


Thr Val Glu Glu Gly Val Glu Ser Leu Ile Glu Ala Ile Lys Asn Leu 
785                 790                 795                 800 


Met Lys Glu Leu Asn Ile Pro Leu Thr Leu Lys Asp Ala Gly Ile Asn 
                805                 810                 815     


Lys Glu Gln Phe Glu Lys Glu Ile Glu Glu Met Ser Asp Ile Ala Phe 
            820                 825                 830         


Asn Asp Gln Cys Thr Gly Thr Asn Pro Arg Met Pro Leu Thr Lys Glu 
        835                 840                 845             


Ile Ala Glu Ile Tyr Arg Lys Ala Tyr Gly Ala 
    850                 855                 


<210>  8
<211>  2580
<212>  DNA
<213>  T.saccharolyticum bifunctional alcohol-acetaldehyde dehydrogenase

<400>  8
atggcaacga caaaaacgga attagacgtt cagaagcaga tagatctact tgtgtcaaga       60

gcacaagagg ctcagaaaaa attcatgtct tacacgcaag agcaaatcga cgcaatagtt      120

aaggcaatgg ctttagcagg cgttgacaaa cacgtagagc tggcaaagat ggcgtacgaa      180

gagacaaaaa tgggtgtata cgaagataag ataacaaaaa atctcttcgc aacagagtac      240

gtgtaccacg acataaaaaa tgaaaagact gtaggaatca taaacgagaa catagaagaa      300

aactacatgg aagtggcaga accgataggc gtaattgccg gtgtcacacc tgtcacaaac      360

ccaacatcta ccacgatgtt taaatgctta atatccataa agacgcgaaa tcctataata      420

ttcagcttcc atccaaaggc aataaagtgc agcatcgcag cagccaaagt gatgtatgaa      480

gctgcactaa aggcaggcgc acctgaagga tgcataggat ggatagaaac gccatcaatt      540

gaggccacac agcttctcat gacacatcca ggcgtatcgc tgatccttgc aacgggcggt      600

gcaggaatgg taaaagcggc atacagctca ggaaaaccgg cattaggcgt aggtcctggc      660

aatgtgccat gctacatcga aaaatcagca aacataaaga gggctgtatc ggatctcata      720

ctaagcaaga catttgacaa tggagtaata tgcgcatcag agcaggccgt aataatagac      780

gaggaaatag cagatgaagt caaaaagctt atgaaagaat acggctgcta cttcttaaac      840

aaagatgaaa taaagaagct tgagaaattt gcaattgatg agcaaagctg cgccatgagc      900

cctgcagtgg taggtcagcc agcggcgaag attgctgaaa tggcaggctt caaagtcccc      960

gaaggcacaa agatattagt ggcagagtac gaaggagtag gtccaaaata tcctctatca     1020

agggagaaac taagcccgat tcttgcttgc tacaccgtca aagactacaa tgaaggaatc     1080

aaaaagtgcg aggaaatgac tgaattcgga ggtttaggcc actctgctgt aatacactct     1140

gaaaatcaaa acgtcataaa tgaatttgca aggcgagtcc gcacaggaag acttatcgta     1200

aattcaccat catcacaggg agcaatagga gatatataca atacaaacac gccatcactt     1260

acattaggct gtggttctat gggaagaaac tcaacgacag acaatgtaag cgtcaagaac     1320

cttttgaata ttaagcgtgt cgtgataagg aatgatagaa tgaaatggtt caagattcca     1380

ccgaagattt actttgaaag cgggtcactc cagtacctgt gcaaagtcaa aagaaaaaaa     1440

gcgtttatcg tcacagatcc attcatggtt aagcttggct tcgtagacaa agtgacatat     1500

caattagaca aagcaaacat cgaatacgaa atattctcag aagtagagcc agatccatct     1560

gttgacacag tcatgaacgg cgtaaaaata atgaattcgt acaatcctga cttaataatc     1620

gctgtaggcg gtggctctgc aatagacgca gcaaagggaa tgtggctttt ctacgaatat     1680

cctgatacag agtttgaaac attgaggctt aaatttgcag acatcagaaa aagggcattt     1740

aagttcccag aacttggcaa aaaagcgcta ttcatcgcaa taccgacaac aagcggcaca     1800

ggctcagaag tgacagcatt tgccgtaata accgacaaaa agagaaacat caagtatcca     1860

ctggcagact acgaacttac acctgacata gccataatag atcctgacct tacaaagact     1920

gtaccgccat ctgtaacagc agacacaggc atggatgtgc tgacacacgc catagaagca     1980

tacgtatcag taatggcatc agactacaca gatgcactgg cggaaaaggc tataaagatc     2040

gtatttgaat acctgccaag ggcttataaa aacggcaatg atgaagaagc ccgcgaaaag     2100

atgcacaatg cttcctgcat ggctggtatg gcattcacaa atgcattctt aggaataaac     2160

cacagcatgg cacacatact gggcggaaag ttccacatac cacacggaag agcaaatgca     2220

atacttctgc cgtatgtaat aaggtacaat gcagaaaaac ctacaaagtt tgtggcattc     2280

ccacaatacg aatatccaaa agcagcagaa agatatgcgg aaatcgccaa attcttagga     2340

ctgcctgctt caactgttga agaaggcgta gaaagcttaa tagaagctat aaagaacctc     2400

atgaaagagc ttaacattcc gcttacactt aaagacgccg gcatcaacaa agaacagttt     2460

gaaaaagaaa tagaggaaat gtcagacatc gccttcaacg atcagtgcac agggacaaac     2520

ccgagaatgc ctctcacaaa agaaattgca gagatctaca gaaaagcata cggtgcatag     2580


<210>  9
<211>  859
<212>  PRT
<213>  T.saccharolyticum bifunctional alcohol-acetaldehyde dehydrogenase

<400>  9

Met Ala Thr Thr Lys Thr Glu Leu Asp Val Gln Lys Gln Ile Asp Leu 
1               5                   10                  15      


Leu Val Ser Arg Ala Gln Glu Ala Gln Lys Lys Phe Met Ser Tyr Thr 
            20                  25                  30          


Gln Glu Gln Ile Asp Ala Ile Val Lys Ala Met Ala Leu Ala Gly Val 
        35                  40                  45              


Asp Lys His Val Glu Leu Ala Lys Met Ala Tyr Glu Glu Thr Lys Met 
    50                  55                  60                  


Gly Val Tyr Glu Asp Lys Ile Thr Lys Asn Leu Phe Ala Thr Glu Tyr 
65                  70                  75                  80  


Val Tyr His Asp Ile Lys Asn Glu Lys Thr Val Gly Ile Ile Asn Glu 
                85                  90                  95      


Asn Ile Glu Glu Asn Tyr Met Glu Val Ala Glu Pro Ile Gly Val Ile 
            100                 105                 110         


Ala Gly Val Thr Pro Val Thr Asn Pro Thr Ser Thr Thr Met Phe Lys 
        115                 120                 125             


Cys Leu Ile Ser Ile Lys Thr Arg Asn Pro Ile Ile Phe Ser Phe His 
    130                 135                 140                 


Pro Lys Ala Ile Lys Cys Ser Ile Ala Ala Ala Lys Val Met Tyr Glu 
145                 150                 155                 160 


Ala Ala Leu Lys Ala Gly Ala Pro Glu Gly Cys Ile Gly Trp Ile Glu 
                165                 170                 175     


Thr Pro Ser Ile Glu Ala Thr Gln Leu Leu Met Thr His Pro Gly Val 
            180                 185                 190         


Ser Leu Ile Leu Ala Thr Gly Gly Ala Gly Met Val Lys Ala Ala Tyr 
        195                 200                 205             


Ser Ser Gly Lys Pro Ala Leu Gly Val Gly Pro Gly Asn Val Pro Cys 
    210                 215                 220                 


Tyr Ile Glu Lys Ser Ala Asn Ile Lys Arg Ala Val Ser Asp Leu Ile 
225                 230                 235                 240 


Leu Ser Lys Thr Phe Asp Asn Gly Val Ile Cys Ala Ser Glu Gln Ala 
                245                 250                 255     


Val Ile Ile Asp Glu Glu Ile Ala Asp Glu Val Lys Lys Leu Met Lys 
            260                 265                 270         


Glu Tyr Gly Cys Tyr Phe Leu Asn Lys Asp Glu Ile Lys Lys Leu Glu 
        275                 280                 285             


Lys Phe Ala Ile Asp Glu Gln Ser Cys Ala Met Ser Pro Ala Val Val 
    290                 295                 300                 


Gly Gln Pro Ala Ala Lys Ile Ala Glu Met Ala Gly Phe Lys Val Pro 
305                 310                 315                 320 


Glu Gly Thr Lys Ile Leu Val Ala Glu Tyr Glu Gly Val Gly Pro Lys 
                325                 330                 335     


Tyr Pro Leu Ser Arg Glu Lys Leu Ser Pro Ile Leu Ala Cys Tyr Thr 
            340                 345                 350         


Val Lys Asp Tyr Asn Glu Gly Ile Lys Lys Cys Glu Glu Met Thr Glu 
        355                 360                 365             


Phe Gly Gly Leu Gly His Ser Ala Val Ile His Ser Glu Asn Gln Asn 
    370                 375                 380                 


Val Ile Asn Glu Phe Ala Arg Arg Val Arg Thr Gly Arg Leu Ile Val 
385                 390                 395                 400 


Asn Ser Pro Ser Ser Gln Gly Ala Ile Gly Asp Ile Tyr Asn Thr Asn 
                405                 410                 415     


Thr Pro Ser Leu Thr Leu Gly Cys Gly Ser Met Gly Arg Asn Ser Thr 
            420                 425                 430         


Thr Asp Asn Val Ser Val Lys Asn Leu Leu Asn Ile Lys Arg Val Val 
        435                 440                 445             


Ile Arg Asn Asp Arg Met Lys Trp Phe Lys Ile Pro Pro Lys Ile Tyr 
    450                 455                 460                 


Phe Glu Ser Gly Ser Leu Gln Tyr Leu Cys Lys Val Lys Arg Lys Lys 
465                 470                 475                 480 


Ala Phe Ile Val Thr Asp Pro Phe Met Val Lys Leu Gly Phe Val Asp 
                485                 490                 495     


Lys Val Thr Tyr Gln Leu Asp Lys Ala Asn Ile Glu Tyr Glu Ile Phe 
            500                 505                 510         


Ser Glu Val Glu Pro Asp Pro Ser Val Asp Thr Val Met Asn Gly Val 
        515                 520                 525             


Lys Ile Met Asn Ser Tyr Asn Pro Asp Leu Ile Ile Ala Val Gly Gly 
    530                 535                 540                 


Gly Ser Ala Ile Asp Ala Ala Lys Gly Met Trp Leu Phe Tyr Glu Tyr 
545                 550                 555                 560 


Pro Asp Thr Glu Phe Glu Thr Leu Arg Leu Lys Phe Ala Asp Ile Arg 
                565                 570                 575     


Lys Arg Ala Phe Lys Phe Pro Glu Leu Gly Lys Lys Ala Leu Phe Ile 
            580                 585                 590         


Ala Ile Pro Thr Thr Ser Gly Thr Gly Ser Glu Val Thr Ala Phe Ala 
        595                 600                 605             


Val Ile Thr Asp Lys Lys Arg Asn Ile Lys Tyr Pro Leu Ala Asp Tyr 
    610                 615                 620                 


Glu Leu Thr Pro Asp Ile Ala Ile Ile Asp Pro Asp Leu Thr Lys Thr 
625                 630                 635                 640 


Val Pro Pro Ser Val Thr Ala Asp Thr Gly Met Asp Val Leu Thr His 
                645                 650                 655     


Ala Ile Glu Ala Tyr Val Ser Val Met Ala Ser Asp Tyr Thr Asp Ala 
            660                 665                 670         


Leu Ala Glu Lys Ala Ile Lys Ile Val Phe Glu Tyr Leu Pro Arg Ala 
        675                 680                 685             


Tyr Lys Asn Gly Asn Asp Glu Glu Ala Arg Glu Lys Met His Asn Ala 
    690                 695                 700                 


Ser Cys Met Ala Gly Met Ala Phe Thr Asn Ala Phe Leu Gly Ile Asn 
705                 710                 715                 720 


His Ser Met Ala His Ile Leu Gly Gly Lys Phe His Ile Pro His Gly 
                725                 730                 735     


Arg Ala Asn Ala Ile Leu Leu Pro Tyr Val Ile Arg Tyr Asn Ala Glu 
            740                 745                 750         


Lys Pro Thr Lys Phe Val Ala Phe Pro Gln Tyr Glu Tyr Pro Lys Ala 
        755                 760                 765             


Ala Glu Arg Tyr Ala Glu Ile Ala Lys Phe Leu Gly Leu Pro Ala Ser 
    770                 775                 780                 


Thr Val Glu Glu Gly Val Glu Ser Leu Ile Glu Ala Ile Lys Asn Leu 
785                 790                 795                 800 


Met Lys Glu Leu Asn Ile Pro Leu Thr Leu Lys Asp Ala Gly Ile Asn 
                805                 810                 815     


Lys Glu Gln Phe Glu Lys Glu Ile Glu Glu Met Ser Asp Ile Ala Phe 
            820                 825                 830         


Asn Asp Gln Cys Thr Gly Thr Asn Pro Arg Met Pro Leu Thr Lys Glu 
        835                 840                 845             


Ile Ala Glu Ile Tyr Arg Lys Ala Tyr Gly Ala 
    850                 855                 


<210>  10
<211>  2580
<212>  DNA
<213>  T.saccharolyticum bifunctional alcohol-acetaldehyde dehydrogenase

<400>  10
atggcaacga caaaaacgga attagacgtt cagaagcaga tagatctact tgtgtcaaga       60

gcacaagagg ctcagaaaaa attcatgtct tacacgcaag agcaaatcga cgcaatagtt      120

aaggcaatgg ctttagcagg cgttgacaaa cacgtagagc tggcaaagat ggcgtacgaa      180

gagacaaaaa tgggtgtata cgaagataag ataacaaaaa atctcttcgc aacagagtac      240

gtgtaccacg acataaaaaa tgaaaagact gtaggaatca taaacgagaa catagaagaa      300

aactacatgg aagtggcaga accgataggc gtaattgccg gtgtcacacc tgtcacaaac      360

ccaacatcta ccacgatgtt taaatgctta atatccataa agacgcgaaa tcctataata      420

ttcagcttcc atccaaaggc aataaagtgc agcatcgcag cagccaaagt gatgtatgaa      480

gctgcactaa aggcaggcgc acctgaagga tgcataggat ggatagaaac gccatcaatt      540

gaggccacac agcttctcat gacacatcca ggcgtatcgc tgatccttgc aacgggcggt      600

gcaggaatgg taaaagcggc atacagctca ggaaaaccgg cattaggcgt aggtcctggc      660

aatgtgccat gctacatcga aaaatcagca aacataaaga gggctgtatc ggatctcata      720

ctaagcaaga catttgacaa tggagtaata tgcgcatcag agcaggccgt aataatagac      780

gaggaaatag cagatgaagt caaaaagctt atgaaagaat acggctgcta cttcttaaac      840

aaagatgaaa taaagaagct tgagaaattt gcaattgatg agcaaagctg cgccatgagc      900

cctgcagtgg taggtcagcc agcggcgaag attgctgaaa tggcaggctt caaagtcccc      960

gaaggcacaa agatattagt ggcagagtac gaaggagtag gtccaaaata tcctctatca     1020

agggagaaac taagcccgat tcttgcttgc tacaccgtca aagactacaa tgaaggaatc     1080

aaaaagtgcg aggaaatgac tgaattcgga ggtttaggcc actctgctgt aatacactct     1140

gaaaatcaaa acgtcataaa tgaatttgca aggcgagtcc gcacaggaag acttatcgta     1200

aattcaccat catcacaggg agcaatagga gatatataca atacaaacac gccatcactt     1260

acattaggct gtggttctat gggaagaaac tcaacgacag acaatgtaag cgtcaagaac     1320

cttttgaata ttaagcgtgt cgtgataagg aaggatagaa tgaaatggtt caagattcca     1380

ccgaagattt actttgaaag cgggtcactc cagtacctgt gcaaagtcaa aagaaaaaaa     1440

gcgtttatcg tcacagatcc attcatggtt aagcttggct tcgtagacaa agtgacatat     1500

caattagaca aagcaaacat cgaatacgaa atattctcag aagtagagcc agatccatct     1560

gttgacacag tcatgaacgg cgtaaaaata atgaattcgt acaatcctga cttaataatc     1620

gctgtaggcg gtggctctgc aatagacgca gcaaagggaa tgtggctttt ctacgaatat     1680

cctgatacag agtttgaaac attgaggctt aaatttgcag acatcagaaa aagggcattt     1740

aagttcccag aacttggcaa aaaagcgcta ttcatcgcaa taccgacaac aagcggcaca     1800

ggcttagaag tgacagcatt tgccgtaata accgacaaaa agagaaacat caagtatcca     1860

ctggcagact acgaacttac acctgacata gccataatag atcctgacct tacaaagact     1920

gtaccgccat ctgtaacagc agacacaggc atggatgtgc tgacacacgc catagaagca     1980

tacgtatcag taatggcatc agactacaca gatgcactgg cggaaaaggc tataaagatc     2040

gtatttgaat acctgccaag ggcttataaa aacggcaatg atgaagaagc ccgcgaaaag     2100

atgcacaatg cttcctgcat ggctggtatg gcattcacaa atgcattctt aggaataaac     2160

cacagcatgg cacacatact gggcggaaag ttccacatac cacacggaag agcaaatgca     2220

atacttctgc cgtatgtaat aaggtacaat gcagaaaaac ctacaaagtt tgtggcattc     2280

ccacaatacg aatatccaaa agcagcagaa agatatgcgg aaatcgccaa attcttagga     2340

ctgcctgctt caactgttga agaaggcgta gaaagcttaa tagaagctat aaagaacctc     2400

atgaaagagc ttaacattcc gcttacactt aaagacgccg gcatcaacaa agaacagttt     2460

gaaaaagaaa tagaggaaat gtcagacatc gccttcaacg atcagtgcac agggacaaac     2520

ccgagaatgc ctctcacaaa agaaattgca gagatctaca gaaaagcata cggtgcatag     2580


<210>  11
<211>  859
<212>  PRT
<213>  T.saccharolyticum bifunctional alcohol-acetaldehyde dehydrogenase

<400>  11

Met Ala Thr Thr Lys Thr Glu Leu Asp Val Gln Lys Gln Ile Asp Leu 
1               5                   10                  15      


Leu Val Ser Arg Ala Gln Glu Ala Gln Lys Lys Phe Met Ser Tyr Thr 
            20                  25                  30          


Gln Glu Gln Ile Asp Ala Ile Val Lys Ala Met Ala Leu Ala Gly Val 
        35                  40                  45              


Asp Lys His Val Glu Leu Ala Lys Met Ala Tyr Glu Glu Thr Lys Met 
    50                  55                  60                  


Gly Val Tyr Glu Asp Lys Ile Thr Lys Asn Leu Phe Ala Thr Glu Tyr 
65                  70                  75                  80  


Val Tyr His Asp Ile Lys Asn Glu Lys Thr Val Gly Ile Ile Asn Glu 
                85                  90                  95      


Asn Ile Glu Glu Asn Tyr Met Glu Val Ala Glu Pro Ile Gly Val Ile 
            100                 105                 110         


Ala Gly Val Thr Pro Val Thr Asn Pro Thr Ser Thr Thr Met Phe Lys 
        115                 120                 125             


Cys Leu Ile Ser Ile Lys Thr Arg Asn Pro Ile Ile Phe Ser Phe His 
    130                 135                 140                 


Pro Lys Ala Ile Lys Cys Ser Ile Ala Ala Ala Lys Val Met Tyr Glu 
145                 150                 155                 160 


Ala Ala Leu Lys Ala Gly Ala Pro Glu Gly Cys Ile Gly Trp Ile Glu 
                165                 170                 175     


Thr Pro Ser Ile Glu Ala Thr Gln Leu Leu Met Thr His Pro Gly Val 
            180                 185                 190         


Ser Leu Ile Leu Ala Thr Gly Gly Ala Gly Met Val Lys Ala Ala Tyr 
        195                 200                 205             


Ser Ser Gly Lys Pro Ala Leu Gly Val Gly Pro Gly Asn Val Pro Cys 
    210                 215                 220                 


Tyr Ile Glu Lys Ser Ala Asn Ile Lys Arg Ala Val Ser Asp Leu Ile 
225                 230                 235                 240 


Leu Ser Lys Thr Phe Asp Asn Gly Val Ile Cys Ala Ser Glu Gln Ala 
                245                 250                 255     


Val Ile Ile Asp Glu Glu Ile Ala Asp Glu Val Lys Lys Leu Met Lys 
            260                 265                 270         


Glu Tyr Gly Cys Tyr Phe Leu Asn Lys Asp Glu Ile Lys Lys Leu Glu 
        275                 280                 285             


Lys Phe Ala Ile Asp Glu Gln Ser Cys Ala Met Ser Pro Ala Val Val 
    290                 295                 300                 


Gly Gln Pro Ala Ala Lys Ile Ala Glu Met Ala Gly Phe Lys Val Pro 
305                 310                 315                 320 


Glu Gly Thr Lys Ile Leu Val Ala Glu Tyr Glu Gly Val Gly Pro Lys 
                325                 330                 335     


Tyr Pro Leu Ser Arg Glu Lys Leu Ser Pro Ile Leu Ala Cys Tyr Thr 
            340                 345                 350         


Val Lys Asp Tyr Asn Glu Gly Ile Lys Lys Cys Glu Glu Met Thr Glu 
        355                 360                 365             


Phe Gly Gly Leu Gly His Ser Ala Val Ile His Ser Glu Asn Gln Asn 
    370                 375                 380                 


Val Ile Asn Glu Phe Ala Arg Arg Val Arg Thr Gly Arg Leu Ile Val 
385                 390                 395                 400 


Asn Ser Pro Ser Ser Gln Gly Ala Ile Gly Asp Ile Tyr Asn Thr Asn 
                405                 410                 415     


Thr Pro Ser Leu Thr Leu Gly Cys Gly Ser Met Gly Arg Asn Ser Thr 
            420                 425                 430         


Thr Asp Asn Val Ser Val Lys Asn Leu Leu Asn Ile Lys Arg Val Val 
        435                 440                 445             


Ile Arg Lys Asp Arg Met Lys Trp Phe Lys Ile Pro Pro Lys Ile Tyr 
    450                 455                 460                 


Phe Glu Ser Gly Ser Leu Gln Tyr Leu Cys Lys Val Lys Arg Lys Lys 
465                 470                 475                 480 


Ala Phe Ile Val Thr Asp Pro Phe Met Val Lys Leu Gly Phe Val Asp 
                485                 490                 495     


Lys Val Thr Tyr Gln Leu Asp Lys Ala Asn Ile Glu Tyr Glu Ile Phe 
            500                 505                 510         


Ser Glu Val Glu Pro Asp Pro Ser Val Asp Thr Val Met Asn Gly Val 
        515                 520                 525             


Lys Ile Met Asn Ser Tyr Asn Pro Asp Leu Ile Ile Ala Val Gly Gly 
    530                 535                 540                 


Gly Ser Ala Ile Asp Ala Ala Lys Gly Met Trp Leu Phe Tyr Glu Tyr 
545                 550                 555                 560 


Pro Asp Thr Glu Phe Glu Thr Leu Arg Leu Lys Phe Ala Asp Ile Arg 
                565                 570                 575     


Lys Arg Ala Phe Lys Phe Pro Glu Leu Gly Lys Lys Ala Leu Phe Ile 
            580                 585                 590         


Ala Ile Pro Thr Thr Ser Gly Thr Gly Leu Glu Val Thr Ala Phe Ala 
        595                 600                 605             


Val Ile Thr Asp Lys Lys Arg Asn Ile Lys Tyr Pro Leu Ala Asp Tyr 
    610                 615                 620                 


Glu Leu Thr Pro Asp Ile Ala Ile Ile Asp Pro Asp Leu Thr Lys Thr 
625                 630                 635                 640 


Val Pro Pro Ser Val Thr Ala Asp Thr Gly Met Asp Val Leu Thr His 
                645                 650                 655     


Ala Ile Glu Ala Tyr Val Ser Val Met Ala Ser Asp Tyr Thr Asp Ala 
            660                 665                 670         


Leu Ala Glu Lys Ala Ile Lys Ile Val Phe Glu Tyr Leu Pro Arg Ala 
        675                 680                 685             


Tyr Lys Asn Gly Asn Asp Glu Glu Ala Arg Glu Lys Met His Asn Ala 
    690                 695                 700                 


Ser Cys Met Ala Gly Met Ala Phe Thr Asn Ala Phe Leu Gly Ile Asn 
705                 710                 715                 720 


His Ser Met Ala His Ile Leu Gly Gly Lys Phe His Ile Pro His Gly 
                725                 730                 735     


Arg Ala Asn Ala Ile Leu Leu Pro Tyr Val Ile Arg Tyr Asn Ala Glu 
            740                 745                 750         


Lys Pro Thr Lys Phe Val Ala Phe Pro Gln Tyr Glu Tyr Pro Lys Ala 
        755                 760                 765             


Ala Glu Arg Tyr Ala Glu Ile Ala Lys Phe Leu Gly Leu Pro Ala Ser 
    770                 775                 780                 


Thr Val Glu Glu Gly Val Glu Ser Leu Ile Glu Ala Ile Lys Asn Leu 
785                 790                 795                 800 


Met Lys Glu Leu Asn Ile Pro Leu Thr Leu Lys Asp Ala Gly Ile Asn 
                805                 810                 815     


Lys Glu Gln Phe Glu Lys Glu Ile Glu Glu Met Ser Asp Ile Ala Phe 
            820                 825                 830         


Asn Asp Gln Cys Thr Gly Thr Asn Pro Arg Met Pro Leu Thr Lys Glu 
        835                 840                 845             


Ile Ala Glu Ile Tyr Arg Lys Ala Tyr Gly Ala 
    850                 855                 


<210>  12
<211>  2580
<212>  DNA
<213>  T.saccharolyticum bifunctional alcohol-acetaldehyde dehydrogenase

<400>  12
atggcaacga caaaaacgga attagacgtt cagaagcaga tagatctact tgtgtcaaga       60

gcacaagagg ctcagaaaaa attcatgtct tacacgcaag agcaaatcga cgcaatagtt      120

aaggcaatgg ctttagcagg cgttgacaaa cacgtagagc tggcaaagat ggcgtacgaa      180

gagacaaaaa tgggtgtata cgaagataag ataacaaaaa atctcttcgc aacagagtac      240

gtgtaccacg acataaaaaa tgaaaagact gtaggaatca taaacgagaa catagaagaa      300

aactacatgg aagtggcaga accgataggc gtaattgccg gtgtcacacc tgtcacaaac      360

ccaacatcta ccacgatgtt taaatgctta atatccataa agacgcgaaa tcctataata      420

ttcagcttcc atccaaaggc aataaagtgc agcatcgcag cagccaaagt gatgtatgaa      480

gctgcactaa aggcaggcgc acctgaagga tgcataggat ggatagaaac gccatcaatt      540

gaggccacac agcttctcat gacacatcca ggcgtatcgc tgatccttgc aacgggcggt      600

gcaggaatgg taaaagcggc atacagctca ggaaaaccgg cattaggcgt aggtcctggc      660

aatgtgccat gctacatcga aaaatcagca aacataaaga gggctgtatc ggatctcata      720

ctaagcaaga catttgacaa tggagtaata tgcgcatcag agcaggccgt aataatagac      780

gaggaaatag cagatgaagt caaaaagctt atgaaagaat acggctgcta cttcttaaac      840

aaagatgaaa taaagaagct tgagaaattt gcaattgatg agcaaagctg cgccatgagc      900

cctgcagtgg taggtcagcc agcggcgaag attgctgaaa tggcaggctt caaagtcccc      960

gaaggcacaa agatattagt ggcagagtac gaaggagtag gtccaaaata tcctctatca     1020

agggagaaac taagcccgat tcttgcttgc tacaccgtca aagactacaa tgaaggaatc     1080

aaaaagtgcg aggaaatgac tgaattcgga ggtttaggcc actctgctgt aatacactct     1140

gaaaatcaaa acgtcataaa tgaatttgca aggcgagtcc gcacaggaag acttatcgta     1200

aattcaccat catcacaggg agcaatagga gatatataca atacaaacac gccatcactt     1260

acattaggct gtggttctat gggaagaaac tcaacgacag acaatgtaag cgtcaagaac     1320

cttttgaata ttaagcgtgt cgtgataagg aaggatagaa tgaaatggtt caagattcca     1380

ccgaagattt actttgaaag cgggtcactc cagtacctgt gcaaagtcaa aagaaaaaaa     1440

gcgtttatcg tcacagatcc attcatggtt aagcttggct tcgtagacaa agtgacatat     1500

caattagaca aagcaaacat cgaatacgaa atattctcag aagtagagcc agatccatct     1560

gttgacacag tcatgaacgg cgtaaaaata atgaattcgt acaatcctga cttaataatc     1620

gctgtaggcg gtggctctgc aatagacgca gcaaagggaa tgtggctttt ctacgaatat     1680

cctgatacag agtttgaaac attgaggctt aaatttgcag acatcagaaa aagggcattt     1740

aagttcccag aacttggcaa aaaagcgcta ttcatcgcaa taccgacaac aagcggcaca     1800

ggctcaggag tgacagcatt tgccgtaata accgacaaaa agagaaacat caagtatcca     1860

ctggcagact acgaacttac acctgacata gccataatag atcctgacct tacaaagact     1920

gtaccgccat ctgtaacagc agacacaggc atggatgtgc tgacacacgc catagaagca     1980

tacgtatcag taatggcatc agactacaca gatgcactgg cggaaaaggc tataaagatc     2040

gtatttgaat acctgccaag ggcttataaa aacggcaatg atgaagaagc ccgcgaaaag     2100

atgcacaatg cttcctgcat ggctggtatg gcattcacaa atgcattctt aggaataaac     2160

cacagcatgg cacacatact gggcggaaag ttccacatac cacacggaag agcaaatgca     2220

atacttctgc cgtatgtaat aaggtacaat gcagaaaaac ctacaaagtt tgtggcattc     2280

ccacaatacg aatatccaaa agcagcagaa agatatgcgg aaatcgccaa attcttagga     2340

ctgcctgctt caactgttga agaaggcgta gaaagcttaa tagaagctat aaagaacctc     2400

atgaaagagc ttaacattcc gcttacactt aaagacgccg gcatcaacaa agaacagttt     2460

gaaaaagaaa tagaggaaat gtcagacatc gccttcaacg atcagtgcac agggacaaac     2520

ccgagaatgc ctctcacaaa agaaattgca gagatctaca gaaaagcata cggtgcatag     2580


<210>  13
<211>  859
<212>  PRT
<213>  T.saccharolyticum bifunctional alcohol-acetaldehyde dehydrogenase

<400>  13

Met Ala Thr Thr Lys Thr Glu Leu Asp Val Gln Lys Gln Ile Asp Leu 
1               5                   10                  15      


Leu Val Ser Arg Ala Gln Glu Ala Gln Lys Lys Phe Met Ser Tyr Thr 
            20                  25                  30          


Gln Glu Gln Ile Asp Ala Ile Val Lys Ala Met Ala Leu Ala Gly Val 
        35                  40                  45              


Asp Lys His Val Glu Leu Ala Lys Met Ala Tyr Glu Glu Thr Lys Met 
    50                  55                  60                  


Gly Val Tyr Glu Asp Lys Ile Thr Lys Asn Leu Phe Ala Thr Glu Tyr 
65                  70                  75                  80  


Val Tyr His Asp Ile Lys Asn Glu Lys Thr Val Gly Ile Ile Asn Glu 
                85                  90                  95      


Asn Ile Glu Glu Asn Tyr Met Glu Val Ala Glu Pro Ile Gly Val Ile 
            100                 105                 110         


Ala Gly Val Thr Pro Val Thr Asn Pro Thr Ser Thr Thr Met Phe Lys 
        115                 120                 125             


Cys Leu Ile Ser Ile Lys Thr Arg Asn Pro Ile Ile Phe Ser Phe His 
    130                 135                 140                 


Pro Lys Ala Ile Lys Cys Ser Ile Ala Ala Ala Lys Val Met Tyr Glu 
145                 150                 155                 160 


Ala Ala Leu Lys Ala Gly Ala Pro Glu Gly Cys Ile Gly Trp Ile Glu 
                165                 170                 175     


Thr Pro Ser Ile Glu Ala Thr Gln Leu Leu Met Thr His Pro Gly Val 
            180                 185                 190         


Ser Leu Ile Leu Ala Thr Gly Gly Ala Gly Met Val Lys Ala Ala Tyr 
        195                 200                 205             


Ser Ser Gly Lys Pro Ala Leu Gly Val Gly Pro Gly Asn Val Pro Cys 
    210                 215                 220                 


Tyr Ile Glu Lys Ser Ala Asn Ile Lys Arg Ala Val Ser Asp Leu Ile 
225                 230                 235                 240 


Leu Ser Lys Thr Phe Asp Asn Gly Val Ile Cys Ala Ser Glu Gln Ala 
                245                 250                 255     


Val Ile Ile Asp Glu Glu Ile Ala Asp Glu Val Lys Lys Leu Met Lys 
            260                 265                 270         


Glu Tyr Gly Cys Tyr Phe Leu Asn Lys Asp Glu Ile Lys Lys Leu Glu 
        275                 280                 285             


Lys Phe Ala Ile Asp Glu Gln Ser Cys Ala Met Ser Pro Ala Val Val 
    290                 295                 300                 


Gly Gln Pro Ala Ala Lys Ile Ala Glu Met Ala Gly Phe Lys Val Pro 
305                 310                 315                 320 


Glu Gly Thr Lys Ile Leu Val Ala Glu Tyr Glu Gly Val Gly Pro Lys 
                325                 330                 335     


Tyr Pro Leu Ser Arg Glu Lys Leu Ser Pro Ile Leu Ala Cys Tyr Thr 
            340                 345                 350         


Val Lys Asp Tyr Asn Glu Gly Ile Lys Lys Cys Glu Glu Met Thr Glu 
        355                 360                 365             


Phe Gly Gly Leu Gly His Ser Ala Val Ile His Ser Glu Asn Gln Asn 
    370                 375                 380                 


Val Ile Asn Glu Phe Ala Arg Arg Val Arg Thr Gly Arg Leu Ile Val 
385                 390                 395                 400 


Asn Ser Pro Ser Ser Gln Gly Ala Ile Gly Asp Ile Tyr Asn Thr Asn 
                405                 410                 415     


Thr Pro Ser Leu Thr Leu Gly Cys Gly Ser Met Gly Arg Asn Ser Thr 
            420                 425                 430         


Thr Asp Asn Val Ser Val Lys Asn Leu Leu Asn Ile Lys Arg Val Val 
        435                 440                 445             


Ile Arg Lys Asp Arg Met Lys Trp Phe Lys Ile Pro Pro Lys Ile Tyr 
    450                 455                 460                 


Phe Glu Ser Gly Ser Leu Gln Tyr Leu Cys Lys Val Lys Arg Lys Lys 
465                 470                 475                 480 


Ala Phe Ile Val Thr Asp Pro Phe Met Val Lys Leu Gly Phe Val Asp 
                485                 490                 495     


Lys Val Thr Tyr Gln Leu Asp Lys Ala Asn Ile Glu Tyr Glu Ile Phe 
            500                 505                 510         


Ser Glu Val Glu Pro Asp Pro Ser Val Asp Thr Val Met Asn Gly Val 
        515                 520                 525             


Lys Ile Met Asn Ser Tyr Asn Pro Asp Leu Ile Ile Ala Val Gly Gly 
    530                 535                 540                 


Gly Ser Ala Ile Asp Ala Ala Lys Gly Met Trp Leu Phe Tyr Glu Tyr 
545                 550                 555                 560 


Pro Asp Thr Glu Phe Glu Thr Leu Arg Leu Lys Phe Ala Asp Ile Arg 
                565                 570                 575     


Lys Arg Ala Phe Lys Phe Pro Glu Leu Gly Lys Lys Ala Leu Phe Ile 
            580                 585                 590         


Ala Ile Pro Thr Thr Ser Gly Thr Gly Ser Gly Val Thr Ala Phe Ala 
        595                 600                 605             


Val Ile Thr Asp Lys Lys Arg Asn Ile Lys Tyr Pro Leu Ala Asp Tyr 
    610                 615                 620                 


Glu Leu Thr Pro Asp Ile Ala Ile Ile Asp Pro Asp Leu Thr Lys Thr 
625                 630                 635                 640 


Val Pro Pro Ser Val Thr Ala Asp Thr Gly Met Asp Val Leu Thr His 
                645                 650                 655     


Ala Ile Glu Ala Tyr Val Ser Val Met Ala Ser Asp Tyr Thr Asp Ala 
            660                 665                 670         


Leu Ala Glu Lys Ala Ile Lys Ile Val Phe Glu Tyr Leu Pro Arg Ala 
        675                 680                 685             


Tyr Lys Asn Gly Asn Asp Glu Glu Ala Arg Glu Lys Met His Asn Ala 
    690                 695                 700                 


Ser Cys Met Ala Gly Met Ala Phe Thr Asn Ala Phe Leu Gly Ile Asn 
705                 710                 715                 720 


His Ser Met Ala His Ile Leu Gly Gly Lys Phe His Ile Pro His Gly 
                725                 730                 735     


Arg Ala Asn Ala Ile Leu Leu Pro Tyr Val Ile Arg Tyr Asn Ala Glu 
            740                 745                 750         


Lys Pro Thr Lys Phe Val Ala Phe Pro Gln Tyr Glu Tyr Pro Lys Ala 
        755                 760                 765             


Ala Glu Arg Tyr Ala Glu Ile Ala Lys Phe Leu Gly Leu Pro Ala Ser 
    770                 775                 780                 


Thr Val Glu Glu Gly Val Glu Ser Leu Ile Glu Ala Ile Lys Asn Leu 
785                 790                 795                 800 


Met Lys Glu Leu Asn Ile Pro Leu Thr Leu Lys Asp Ala Gly Ile Asn 
                805                 810                 815     


Lys Glu Gln Phe Glu Lys Glu Ile Glu Glu Met Ser Asp Ile Ala Phe 
            820                 825                 830         


Asn Asp Gln Cys Thr Gly Thr Asn Pro Arg Met Pro Leu Thr Lys Glu 
        835                 840                 845             


Ile Ala Glu Ile Tyr Arg Lys Ala Tyr Gly Ala 
    850                 855                 


<210>  14
<211>  2580
<212>  DNA
<213>  T.saccharolyticum bifunctional alcohol-acetaldehyde dehydrogenase

<400>  14
atggcaacga caaaaacgga attagacgtt cagaagcaga tagatctact tgtgtcaaga       60

gcacaagagg ctcagaaaaa attcatgtct tacacgcaag agcaaatcga cgcaatagtt      120

aaggcaatgg ctttagcagg cgttgacaaa cacgtagagc tggcaaagat ggcgtacgaa      180

gagacaaaaa tgggtgtata cgaagataag ataacaaaaa atctcttcgc aacagagtac      240

gtgtaccacg acataaaaaa tgaaaagact gtaggaatca taaacgagaa catagaagaa      300

aactacatgg aagtggcaga accgataggc gtaattgccg gtgtcacacc tgtcacaaac      360

ccaacatcta ccacgatgtt taaatgctta atatccataa agacgcgaaa tcctataata      420

ttcagcttcc atccaaaggc aataaagtgc agcatcgcag cagccaaagt gatgtatgaa      480

gctgcactaa aggcaggcgc acctgaagga tgcataggat ggatagaaac gccatcaatt      540

gaggccacac agcttctcat gacacatcca ggcgtatcgc tgatccttgc aacgggcggt      600

gcaggaatgg taaaagcggc atacagctca ggaaaaccgg cattaggcgt aggtcctggc      660

aatgtgccat gctacatcga aaaatcagca aacataaaga gggctgtatc ggatctcata      720

ctaagcaaga catttgacaa tggagtaata tgcgcatcag agcaggccgt aataatagac      780

gaggaaatag cagatgaagt caaaaagctt atgaaagaat acggctgcta cttcttaaac      840

aaagatgaaa taaagaagct tgagaaattt gcaattgatg agcaaagctg cgccatgagc      900

cctgcagtgg taggtcagcc agcggcgaag attgctgaaa tggcaggctt caaagtcccc      960

gaaggcacaa agatattagt ggcagagtac gaaggagtag gtccaaaata tcctctatca     1020

agggagaaac taagcccgat tcttgcttgc tacaccgtca aagactacaa tgaaggaatc     1080

aaaaagtgcg aggaaatgac tgaattcgga ggtttaggcc actctgctgt aatacactct     1140

gaaaatcaaa acgtcataaa tgaatttgca aggcgagtcc gcacaggaag acttatcgta     1200

aattcaccat catcacaggg agcaatagga gatatataca atacaaacac gccatcactt     1260

acattaggct gtggttctat gggaagaaac tcaacgacag acaatgtaag cgtcaagaac     1320

cttttgaata ttaagcgtgt cgtgataagg aaggatagaa tgaaatggtt caagattcca     1380

ccgaagattt actttgaaag cgggtcactc cagtacctgt gcaaagtcaa aagaaaaaaa     1440

gcgtttatcg tcacagatcc attcatggtt aagcttggct tcgtagacaa agtgacatat     1500

caattagaca aagcaaacat cgaatacgaa atattctcag aagtagagcc agatccatct     1560

gttgacacag tcatgaacgg cgtaaaaata atgaattcgt acaatcctga cttaataatc     1620

gctgtaggcg atggctctgc aatagacgca gcaaagggaa tgtggctttt ctacgaatat     1680

cctgatacag agtttgaaac attgaggctt aaatttgcag acatcagaaa aagggcattt     1740

aagttcccag aacttggcaa aaaagcgcta ttcatcgcaa taccgacaac aagcggcaca     1800

ggctcagaag tgacagcatt tgccgtaata accgacaaaa agagaaacat caagtatcca     1860

ctggcagact acgaacttac acctgacata gccataatag atcctgacct tacaaagact     1920

gtaccgccat ctgtaacagc agacacaggc atggatgtgc tgacacacgc catagaagca     1980

tacgtatcag taatggcatc agactacaca gatgcactgg cggaaaaggc tataaagatc     2040

gtatttgaat acctgccaag ggcttataaa aacggcaatg atgaagaagc ccgcgaaaag     2100

atgcacaatg cttcctgcat ggctggtatg gcattcacaa atgcattctt aggaataaac     2160

cacagcatgg cacacatact gggcggaaag ttccacatac cacacggaag agcaaatgca     2220

atacttctgc cgtatgtaat aaggtacaat gcagaaaaac ctacaaagtt tgtggcattc     2280

ccacaatacg aatatccaaa agcagcagaa agatatgcgg aaatcgccaa attcttagga     2340

ctgcctgctt caactgttga agaaggcgta gaaagcttaa tagaagctat aaagaacctc     2400

atgaaagagc ttaacattcc gcttacactt aaagacgccg gcatcaacaa agaacagttt     2460

gaaaaagaaa tagaggaaat gtcagacatc gccttcaacg atcagtgcac agggacaaac     2520

ccgagaatgc ctctcacaaa agaaattgca gagatctaca gaaaagcata cggtgcatag     2580


<210>  15
<211>  859
<212>  PRT
<213>  T.saccharolyticum bifunctional alcohol-acetaldehyde dehydrogenase

<400>  15

Met Ala Thr Thr Lys Thr Glu Leu Asp Val Gln Lys Gln Ile Asp Leu 
1               5                   10                  15      


Leu Val Ser Arg Ala Gln Glu Ala Gln Lys Lys Phe Met Ser Tyr Thr 
            20                  25                  30          


Gln Glu Gln Ile Asp Ala Ile Val Lys Ala Met Ala Leu Ala Gly Val 
        35                  40                  45              


Asp Lys His Val Glu Leu Ala Lys Met Ala Tyr Glu Glu Thr Lys Met 
    50                  55                  60                  


Gly Val Tyr Glu Asp Lys Ile Thr Lys Asn Leu Phe Ala Thr Glu Tyr 
65                  70                  75                  80  


Val Tyr His Asp Ile Lys Asn Glu Lys Thr Val Gly Ile Ile Asn Glu 
                85                  90                  95      


Asn Ile Glu Glu Asn Tyr Met Glu Val Ala Glu Pro Ile Gly Val Ile 
            100                 105                 110         


Ala Gly Val Thr Pro Val Thr Asn Pro Thr Ser Thr Thr Met Phe Lys 
        115                 120                 125             


Cys Leu Ile Ser Ile Lys Thr Arg Asn Pro Ile Ile Phe Ser Phe His 
    130                 135                 140                 


Pro Lys Ala Ile Lys Cys Ser Ile Ala Ala Ala Lys Val Met Tyr Glu 
145                 150                 155                 160 


Ala Ala Leu Lys Ala Gly Ala Pro Glu Gly Cys Ile Gly Trp Ile Glu 
                165                 170                 175     


Thr Pro Ser Ile Glu Ala Thr Gln Leu Leu Met Thr His Pro Gly Val 
            180                 185                 190         


Ser Leu Ile Leu Ala Thr Gly Gly Ala Gly Met Val Lys Ala Ala Tyr 
        195                 200                 205             


Ser Ser Gly Lys Pro Ala Leu Gly Val Gly Pro Gly Asn Val Pro Cys 
    210                 215                 220                 


Tyr Ile Glu Lys Ser Ala Asn Ile Lys Arg Ala Val Ser Asp Leu Ile 
225                 230                 235                 240 


Leu Ser Lys Thr Phe Asp Asn Gly Val Ile Cys Ala Ser Glu Gln Ala 
                245                 250                 255     


Val Ile Ile Asp Glu Glu Ile Ala Asp Glu Val Lys Lys Leu Met Lys 
            260                 265                 270         


Glu Tyr Gly Cys Tyr Phe Leu Asn Lys Asp Glu Ile Lys Lys Leu Glu 
        275                 280                 285             


Lys Phe Ala Ile Asp Glu Gln Ser Cys Ala Met Ser Pro Ala Val Val 
    290                 295                 300                 


Gly Gln Pro Ala Ala Lys Ile Ala Glu Met Ala Gly Phe Lys Val Pro 
305                 310                 315                 320 


Glu Gly Thr Lys Ile Leu Val Ala Glu Tyr Glu Gly Val Gly Pro Lys 
                325                 330                 335     


Tyr Pro Leu Ser Arg Glu Lys Leu Ser Pro Ile Leu Ala Cys Tyr Thr 
            340                 345                 350         


Val Lys Asp Tyr Asn Glu Gly Ile Lys Lys Cys Glu Glu Met Thr Glu 
        355                 360                 365             


Phe Gly Gly Leu Gly His Ser Ala Val Ile His Ser Glu Asn Gln Asn 
    370                 375                 380                 


Val Ile Asn Glu Phe Ala Arg Arg Val Arg Thr Gly Arg Leu Ile Val 
385                 390                 395                 400 


Asn Ser Pro Ser Ser Gln Gly Ala Ile Gly Asp Ile Tyr Asn Thr Asn 
                405                 410                 415     


Thr Pro Ser Leu Thr Leu Gly Cys Gly Ser Met Gly Arg Asn Ser Thr 
            420                 425                 430         


Thr Asp Asn Val Ser Val Lys Asn Leu Leu Asn Ile Lys Arg Val Val 
        435                 440                 445             


Ile Arg Lys Asp Arg Met Lys Trp Phe Lys Ile Pro Pro Lys Ile Tyr 
    450                 455                 460                 


Phe Glu Ser Gly Ser Leu Gln Tyr Leu Cys Lys Val Lys Arg Lys Lys 
465                 470                 475                 480 


Ala Phe Ile Val Thr Asp Pro Phe Met Val Lys Leu Gly Phe Val Asp 
                485                 490                 495     


Lys Val Thr Tyr Gln Leu Asp Lys Ala Asn Ile Glu Tyr Glu Ile Phe 
            500                 505                 510         


Ser Glu Val Glu Pro Asp Pro Ser Val Asp Thr Val Met Asn Gly Val 
        515                 520                 525             


Lys Ile Met Asn Ser Tyr Asn Pro Asp Leu Ile Ile Ala Val Gly Asp 
    530                 535                 540                 


Gly Ser Ala Ile Asp Ala Ala Lys Gly Met Trp Leu Phe Tyr Glu Tyr 
545                 550                 555                 560 


Pro Asp Thr Glu Phe Glu Thr Leu Arg Leu Lys Phe Ala Asp Ile Arg 
                565                 570                 575     


Lys Arg Ala Phe Lys Phe Pro Glu Leu Gly Lys Lys Ala Leu Phe Ile 
            580                 585                 590         


Ala Ile Pro Thr Thr Ser Gly Thr Gly Ser Glu Val Thr Ala Phe Ala 
        595                 600                 605             


Val Ile Thr Asp Lys Lys Arg Asn Ile Lys Tyr Pro Leu Ala Asp Tyr 
    610                 615                 620                 


Glu Leu Thr Pro Asp Ile Ala Ile Ile Asp Pro Asp Leu Thr Lys Thr 
625                 630                 635                 640 


Val Pro Pro Ser Val Thr Ala Asp Thr Gly Met Asp Val Leu Thr His 
                645                 650                 655     


Ala Ile Glu Ala Tyr Val Ser Val Met Ala Ser Asp Tyr Thr Asp Ala 
            660                 665                 670         


Leu Ala Glu Lys Ala Ile Lys Ile Val Phe Glu Tyr Leu Pro Arg Ala 
        675                 680                 685             


Tyr Lys Asn Gly Asn Asp Glu Glu Ala Arg Glu Lys Met His Asn Ala 
    690                 695                 700                 


Ser Cys Met Ala Gly Met Ala Phe Thr Asn Ala Phe Leu Gly Ile Asn 
705                 710                 715                 720 


His Ser Met Ala His Ile Leu Gly Gly Lys Phe His Ile Pro His Gly 
                725                 730                 735     


Arg Ala Asn Ala Ile Leu Leu Pro Tyr Val Ile Arg Tyr Asn Ala Glu 
            740                 745                 750         


Lys Pro Thr Lys Phe Val Ala Phe Pro Gln Tyr Glu Tyr Pro Lys Ala 
        755                 760                 765             


Ala Glu Arg Tyr Ala Glu Ile Ala Lys Phe Leu Gly Leu Pro Ala Ser 
    770                 775                 780                 


Thr Val Glu Glu Gly Val Glu Ser Leu Ile Glu Ala Ile Lys Asn Leu 
785                 790                 795                 800 


Met Lys Glu Leu Asn Ile Pro Leu Thr Leu Lys Asp Ala Gly Ile Asn 
                805                 810                 815     


Lys Glu Gln Phe Glu Lys Glu Ile Glu Glu Met Ser Asp Ile Ala Phe 
            820                 825                 830         


Asn Asp Gln Cys Thr Gly Thr Asn Pro Arg Met Pro Leu Thr Lys Glu 
        835                 840                 845             


Ile Ala Glu Ile Tyr Arg Lys Ala Tyr Gly Ala 
    850                 855                 


<210>  16
<211>  2622
<212>  DNA
<213>  C.thermocellum bifunctional alcohol-acetaldehyde dehydrogenase

<400>  16
atgacgaaaa tagcgaataa atacgaagtt attgataatg ttgaaaagct tgaaaaggct       60

ttgaaacgtt taagagaagc tcaaagtgtt tatgcaacct atacacagga gcaggttgac      120

aaaattttct ttgaggcggc aatggcggcc aataaaatga gaattcctct tgccaaaatg      180

gctgtggagg aaacaggcat gggagtggtt gaagacaagg ttatcaaaaa ccactatgct      240

tctgagtata tctataatgc gtacaaaaac actaaaacct gcggtgttat tgaagaggac      300

cctgctttcg gtattaaaaa aatagcagag cctttggggg ttattgcggc ggttatacct      360

actacgaatc cgacatcgac agcaatcttt aagactctta ttgcattaaa gacgagaaat      420

gcaattatta taagcccaca ccccagggca aaaaactcaa cgatagaagc ggcgaaaatt      480

gttttggagg cggccgttaa agccggtgct ccggaaggca tcattggctg gattgatgtg      540

ccgagccttg aacttaccaa cctggtaatg agagaagcag atgtgattct cgcaacaggc      600

ggtcccggac tggttaaagc agcttactct tcgggcaaac cggctattgg tgtcggagcg      660

ggcaatactc ctgcaattat tgatgattcg gccgacattg tcttggcagt gaactcaata      720

atacattcaa aaactttcga caacggtatg atttgtgctt cagagcaatc ggtcattgtt      780

ctggacgggg tatataaaga ggtaaaaaaa gaatttgaaa aaagaggatg ctatttctta      840

aatgaagatg aaactgaaaa ggtaagaaaa acaattataa taaacggtgc gttaaatgcc      900

aagatagtag gtcagaaagc tcacacaatt gcaaaccttg caggttttga ggtacccgag      960

actacaaaaa ttctgatagg cgaagttacc agcgtggata tttccgaaga atttgcccac     1020

gaaaagttgt gcccggtact ggcaatgtac agggcaaagg attttgacga tgcccttgat     1080

aaagcagaaa ggttggtagc tgacggtgga tttggccata cttcgtcact ttatatagat     1140

acggtaacac agaaagagaa acttcagaaa ttctctgaaa ggatgaaaac ctgccgtata     1200

ttggttaata cgccgtcatc ccagggaggt atcggtgacc tttacaactt caagcttgct     1260

ccgtctctca ccctcggctg cggttcctgg ggaggaaatt cagtttccga caatgtggga     1320

gtcaagcatt tgttaaacat taaaacagtt gccgagagga gagagaacat gctctggttc     1380

agaacacctg aaaagattta tataaaaaga ggttgtctgc ctgttgcatt ggatgagctt     1440

aaaaatgtaa tgggtaaaaa gaaagcattt attgtaacgg ataacttcct gtacaataac     1500

ggctacacca agccgattac ggataagctg gatgaaatgg gaattgtgca caagaccttc     1560

tttgatgtgt ctccagaccc atcccttgca tctgccaaag ccggtgcggc agaaatgctg     1620

gctttccagc ctgacaccat aattgcggtc ggcggcggat ctgccatgga cgcggccaaa     1680

atcatgtggg tgatgtatga acatccggaa gttgacttta tggacatggc aatgagattt     1740

atggatataa gaaagagagt ttacaccttc ccgaagatgg gacagaaggc atactttatc     1800

gcaattccga cttccgcggg tacaggttca gaagtgacac cttttgcggt tattactgat     1860

gaaaaaacag gaattaaata ccctctggcc gactatgaat tgttgccgga catggctatt     1920

gtagatgccg atatgatgat gaatgctcca aagggactta ccgcagcttc cggtatagac     1980

gcattgaccc acgctctgga agcctatgtt tcaatgcttg cgaccgacta tacggatagc     2040

cttgcccttc gtgcaataaa gatgatattt gaatatctcc cgagagccta tgaaaacggt     2100

gcaagtgacc tggttgcaag agagaaaatg gccaatgccg caacaatagc cggaatggct     2160

tttgccaatg cctttttggg tgtatgccat tcaatggcgc gcaaactggg tgctttttat     2220

cacctgcccc acggtgttgc caatgcactt atgataaacg aagtaatcag attcaactca     2280

tccgaggctc cgaccaagat gggtactttc ccgcagtatg accatccgcg cacgctggaa     2340

aggtatgcag aaattgccga ttatatcgga cttaagggca agaataacga agaaaaagtt     2400

gaaaacttga ttaaagctat tgatgagctt aaagaaaagg tgggcatcag gaagaccatc     2460

aaagattatg acatagatga aaaggaattt ttggacagac tggacgaaat ggtggaacag     2520

gcttttgacg accagtgcac aggtacaaat ccaagatacc cgcttatgaa tgaaatcagg     2580

caaatgtatc tgaacgctta ttacggaggt gcgaagaaat ga                        2622


<210>  17
<211>  873
<212>  PRT
<213>  C.thermocellum bifunctional alcohol-acetaldehyde dehydrogenase

<400>  17

Met Thr Lys Ile Ala Asn Lys Tyr Glu Val Ile Asp Asn Val Glu Lys 
1               5                   10                  15      


Leu Glu Lys Ala Leu Lys Arg Leu Arg Glu Ala Gln Ser Val Tyr Ala 
            20                  25                  30          


Thr Tyr Thr Gln Glu Gln Val Asp Lys Ile Phe Phe Glu Ala Ala Met 
        35                  40                  45              


Ala Ala Asn Lys Met Arg Ile Pro Leu Ala Lys Met Ala Val Glu Glu 
    50                  55                  60                  


Thr Gly Met Gly Val Val Glu Asp Lys Val Ile Lys Asn His Tyr Ala 
65                  70                  75                  80  


Ser Glu Tyr Ile Tyr Asn Ala Tyr Lys Asn Thr Lys Thr Cys Gly Val 
                85                  90                  95      


Ile Glu Glu Asp Pro Ala Phe Gly Ile Lys Lys Ile Ala Glu Pro Leu 
            100                 105                 110         


Gly Val Ile Ala Ala Val Ile Pro Thr Thr Asn Pro Thr Ser Thr Ala 
        115                 120                 125             


Ile Phe Lys Thr Leu Ile Ala Leu Lys Thr Arg Asn Ala Ile Ile Ile 
    130                 135                 140                 


Ser Pro His Pro Arg Ala Lys Asn Ser Thr Ile Glu Ala Ala Lys Ile 
145                 150                 155                 160 


Val Leu Glu Ala Ala Val Lys Ala Gly Ala Pro Glu Gly Ile Ile Gly 
                165                 170                 175     


Trp Ile Asp Val Pro Ser Leu Glu Leu Thr Asn Leu Val Met Arg Glu 
            180                 185                 190         


Ala Asp Val Ile Leu Ala Thr Gly Gly Pro Gly Leu Val Lys Ala Ala 
        195                 200                 205             


Tyr Ser Ser Gly Lys Pro Ala Ile Gly Val Gly Ala Gly Asn Thr Pro 
    210                 215                 220                 


Ala Ile Ile Asp Asp Ser Ala Asp Ile Val Leu Ala Val Asn Ser Ile 
225                 230                 235                 240 


Ile His Ser Lys Thr Phe Asp Asn Gly Met Ile Cys Ala Ser Glu Gln 
                245                 250                 255     


Ser Val Ile Val Leu Asp Gly Val Tyr Lys Glu Val Lys Lys Glu Phe 
            260                 265                 270         


Glu Lys Arg Gly Cys Tyr Phe Leu Asn Glu Asp Glu Thr Glu Lys Val 
        275                 280                 285             


Arg Lys Thr Ile Ile Ile Asn Gly Ala Leu Asn Ala Lys Ile Val Gly 
    290                 295                 300                 


Gln Lys Ala His Thr Ile Ala Asn Leu Ala Gly Phe Glu Val Pro Glu 
305                 310                 315                 320 


Thr Thr Lys Ile Leu Ile Gly Glu Val Thr Ser Val Asp Ile Ser Glu 
                325                 330                 335     


Glu Phe Ala His Glu Lys Leu Cys Pro Val Leu Ala Met Tyr Arg Ala 
            340                 345                 350         


Lys Asp Phe Asp Asp Ala Leu Asp Lys Ala Glu Arg Leu Val Ala Asp 
        355                 360                 365             


Gly Gly Phe Gly His Thr Ser Ser Leu Tyr Ile Asp Thr Val Thr Gln 
    370                 375                 380                 


Lys Glu Lys Leu Gln Lys Phe Ser Glu Arg Met Lys Thr Cys Arg Ile 
385                 390                 395                 400 


Leu Val Asn Thr Pro Ser Ser Gln Gly Gly Ile Gly Asp Leu Tyr Asn 
                405                 410                 415     


Phe Lys Leu Ala Pro Ser Leu Thr Leu Gly Cys Gly Ser Trp Gly Gly 
            420                 425                 430         


Asn Ser Val Ser Asp Asn Val Gly Val Lys His Leu Leu Asn Ile Lys 
        435                 440                 445             


Thr Val Ala Glu Arg Arg Glu Asn Met Leu Trp Phe Arg Thr Pro Glu 
    450                 455                 460                 


Lys Ile Tyr Ile Lys Arg Gly Cys Leu Pro Val Ala Leu Asp Glu Leu 
465                 470                 475                 480 


Lys Asn Val Met Gly Lys Lys Lys Ala Phe Ile Val Thr Asp Asn Phe 
                485                 490                 495     


Leu Tyr Asn Asn Gly Tyr Thr Lys Pro Ile Thr Asp Lys Leu Asp Glu 
            500                 505                 510         


Met Gly Ile Val His Lys Thr Phe Phe Asp Val Ser Pro Asp Pro Ser 
        515                 520                 525             


Leu Ala Ser Ala Lys Ala Gly Ala Ala Glu Met Leu Ala Phe Gln Pro 
    530                 535                 540                 


Asp Thr Ile Ile Ala Val Gly Gly Gly Ser Ala Met Asp Ala Ala Lys 
545                 550                 555                 560 


Ile Met Trp Val Met Tyr Glu His Pro Glu Val Asp Phe Met Asp Met 
                565                 570                 575     


Ala Met Arg Phe Met Asp Ile Arg Lys Arg Val Tyr Thr Phe Pro Lys 
            580                 585                 590         


Met Gly Gln Lys Ala Tyr Phe Ile Ala Ile Pro Thr Ser Ala Gly Thr 
        595                 600                 605             


Gly Ser Glu Val Thr Pro Phe Ala Val Ile Thr Asp Glu Lys Thr Gly 
    610                 615                 620                 


Ile Lys Tyr Pro Leu Ala Asp Tyr Glu Leu Leu Pro Asp Met Ala Ile 
625                 630                 635                 640 


Val Asp Ala Asp Met Met Met Asn Ala Pro Lys Gly Leu Thr Ala Ala 
                645                 650                 655     


Ser Gly Ile Asp Ala Leu Thr His Ala Leu Glu Ala Tyr Val Ser Met 
            660                 665                 670         


Leu Ala Thr Asp Tyr Thr Asp Ser Leu Ala Leu Arg Ala Ile Lys Met 
        675                 680                 685             


Ile Phe Glu Tyr Leu Pro Arg Ala Tyr Glu Asn Gly Ala Ser Asp Leu 
    690                 695                 700                 


Val Ala Arg Glu Lys Met Ala Asn Ala Ala Thr Ile Ala Gly Met Ala 
705                 710                 715                 720 


Phe Ala Asn Ala Phe Leu Gly Val Cys His Ser Met Ala Arg Lys Leu 
                725                 730                 735     


Gly Ala Phe Tyr His Leu Pro His Gly Val Ala Asn Ala Leu Met Ile 
            740                 745                 750         


Asn Glu Val Ile Arg Phe Asn Ser Ser Glu Ala Pro Thr Lys Met Gly 
        755                 760                 765             


Thr Phe Pro Gln Tyr Asp His Pro Arg Thr Leu Glu Arg Tyr Ala Glu 
    770                 775                 780                 


Ile Ala Asp Tyr Ile Gly Leu Lys Gly Lys Asn Asn Glu Glu Lys Val 
785                 790                 795                 800 


Glu Asn Leu Ile Lys Ala Ile Asp Glu Leu Lys Glu Lys Val Gly Ile 
                805                 810                 815     


Arg Lys Thr Ile Lys Asp Tyr Asp Ile Asp Glu Lys Glu Phe Leu Asp 
            820                 825                 830         


Arg Leu Asp Glu Met Val Glu Gln Ala Phe Asp Asp Gln Cys Thr Gly 
        835                 840                 845             


Thr Asn Pro Arg Tyr Pro Leu Met Asn Glu Ile Arg Gln Met Tyr Leu 
    850                 855                 860                 


Asn Ala Tyr Tyr Gly Gly Ala Lys Lys 
865                 870             


<210>  18
<211>  2622
<212>  DNA
<213>  C.thermocellum bifunctional alcohol-acetaldehyde dehydrogenase

<400>  18
atgacgaaaa tagcgaataa atacgaagtt attgataatg ttgaaaagct tgaaaaggct       60

ttgaaacgtt taagagaagc tcaaagtgtt tatgcaacct atacacagga gcaggttgac      120

aaaattttct ttgaggcggc aatggcggcc aataaaatga gaattcctct tgccaaaatg      180

gctgtggagg aaacaggcat gggagtggtt gaagacaagg ttatcaaaaa ccactatgct      240

tctgagtata tctataatgc gtacaaaaac actaaaacct gcggtgttat tgaagaggac      300

cctgctttcg gtattaaaaa aatagcagag cctttggggg ttattgcggc ggttatacct      360

actacgaatc cgacatcgac agcaatcttt aagactctta ttgcattaaa gacgagaaat      420

gcaattatta taagcccaca ccccagggca aaaaactcaa cgatagaagc ggcgaaaatt      480

gttttggagg cggccgttaa agccggtgct ccggaaggca tcattggctg gattgatgtg      540

ccgagccttg aacttaccaa cctggtaatg agagaagcag atgtgattct cgcaacaggc      600

ggtcccggac tggttaaagc agcttactct tcgggcaaac cggctattgg tgtcggagcg      660

ggcaatactc ctgcaattat tgatgattcg gccgacattg tcttggcagt gaactcaata      720

atacattcaa aaactttcga caacggtatg atttgtgctt cagagcaatc ggtcattgtt      780

ctggacgggg tatataaaga ggtaaaaaaa gaatttgaaa aaagaggatg ctatttctta      840

aatgaagatg aaactgaaaa ggtaagaaaa acaattataa taaacggtgc gttaaatgcc      900

aagatagtag gtcagaaagc tcacacaatt gcaaaccttg caggttttga ggtacccgag      960

actacaaaaa ttctgatagg cgaagttacc agcgtggata tttccgaaga atttgcccac     1020

gaaaagttgt gcccggtact ggcaatgtac agggcaaagg attttgacga tgcccttgat     1080

aaagcagaaa ggttggtagc tgacggtgga tttggccata cttcgtcact ttatatagat     1140

acggtaacac agaaagagaa acttcagaaa ttctctgaaa ggatgaaaac ctgccgtata     1200

ttggttaata cgccgtcatc ccagggaggt atcggtgacc tttacaactt caagcttgct     1260

ccgtctctca ccctcggctg cggttcctgg ggaggaaatt cagtttccga caatgtggga     1320

gtcaagcatt tgttaaacat taaaacagtt gccgagagga gagagaacat gctctggttc     1380

agaacacctg aaaagattta tataaaaaga ggttgtctgc ctgttgcatt ggatgagctt     1440

aaaaatgtaa tgggtaaaaa gaaagcattt attgtaacgg ataacttcct gtacaataac     1500

ggctacacca agccgattac ggataagctg gatgaaatgg gaattgtgca caagaccttc     1560

tttgatgtgt ctccagaccc atcccttgca tctgccaaag ccggtgcggc agaaatgctg     1620

gctttccagc ctgacaccat aattgcggtc ggcggcagat ctgccatgga cgcggccaaa     1680

atcatgtggg tgatgtatga acatccggaa gttgacttta tggacatggc aatgagattt     1740

atggatataa gaaagagagt ttacaccttc ccgaagatgg gacagaaggc atactttatc     1800

gcaattccga cttccgcggg tacaggttca gaagtgacac cttttgcggt tattactgat     1860

gaaaaaacag gaattaaata ccctctggcc gactatgaat tgttgccgga catggctatt     1920

gtagatgccg atatgatgat gaatgctcca aagggactta ccgcagcttc cggtatagac     1980

gcattgaccc acgctctgga agcctatgtt tcaatgcttg cgaccgacta tacggatagc     2040

cttgcccttc gtgcaataaa gatgatattt gaatatctcc cgagagccta tgaaaacggt     2100

gcaagtgacc cggttgcaag agagaaaatg gccaatgccg caacaatagc cggaatggct     2160

tttgccaatg cctttttggg tgtatgccat tcaatggcgc acaaactggg tgctttttat     2220

cacctgcccc acggtgttgc caatgcactt atgataaacg aagtaatcag attcaactca     2280

tccgaggctc cgaccaagat gggtactttc ccgcagtatg accatccgcg cacgctggaa     2340

aggtatgcag aaattgccga ttatatcgga cttaagggca agaataacga agaaaaagtt     2400

gaaaacttga ttaaagctat tgatgagctt aaagaaaagg tgggcatcag gaagaccatc     2460

aaagattatg acatagatga aaaggaattt ttggacagac tggacgaaat ggtggaacag     2520

gcttttgacg accagtgcac aggtacaaat ccaagatacc cgcttatgaa tgaaatcagg     2580

caaatgtatc tgaacgctta ttacggaggt gcgaagaaat ga                        2622


<210>  19
<211>  873
<212>  PRT
<213>  C.thermocellum bifunctional alcohol-acetaldehyde dehydrogenase

<400>  19

Met Thr Lys Ile Ala Asn Lys Tyr Glu Val Ile Asp Asn Val Glu Lys 
1               5                   10                  15      


Leu Glu Lys Ala Leu Lys Arg Leu Arg Glu Ala Gln Ser Val Tyr Ala 
            20                  25                  30          


Thr Tyr Thr Gln Glu Gln Val Asp Lys Ile Phe Phe Glu Ala Ala Met 
        35                  40                  45              


Ala Ala Asn Lys Met Arg Ile Pro Leu Ala Lys Met Ala Val Glu Glu 
    50                  55                  60                  


Thr Gly Met Gly Val Val Glu Asp Lys Val Ile Lys Asn His Tyr Ala 
65                  70                  75                  80  


Ser Glu Tyr Ile Tyr Asn Ala Tyr Lys Asn Thr Lys Thr Cys Gly Val 
                85                  90                  95      


Ile Glu Glu Asp Pro Ala Phe Gly Ile Lys Lys Ile Ala Glu Pro Leu 
            100                 105                 110         


Gly Val Ile Ala Ala Val Ile Pro Thr Thr Asn Pro Thr Ser Thr Ala 
        115                 120                 125             


Ile Phe Lys Thr Leu Ile Ala Leu Lys Thr Arg Asn Ala Ile Ile Ile 
    130                 135                 140                 


Ser Pro His Pro Arg Ala Lys Asn Ser Thr Ile Glu Ala Ala Lys Ile 
145                 150                 155                 160 


Val Leu Glu Ala Ala Val Lys Ala Gly Ala Pro Glu Gly Ile Ile Gly 
                165                 170                 175     


Trp Ile Asp Val Pro Ser Leu Glu Leu Thr Asn Leu Val Met Arg Glu 
            180                 185                 190         


Ala Asp Val Ile Leu Ala Thr Gly Gly Pro Gly Leu Val Lys Ala Ala 
        195                 200                 205             


Tyr Ser Ser Gly Lys Pro Ala Ile Gly Val Gly Ala Gly Asn Thr Pro 
    210                 215                 220                 


Ala Ile Ile Asp Asp Ser Ala Asp Ile Val Leu Ala Val Asn Ser Ile 
225                 230                 235                 240 


Ile His Ser Lys Thr Phe Asp Asn Gly Met Ile Cys Ala Ser Glu Gln 
                245                 250                 255     


Ser Val Ile Val Leu Asp Gly Val Tyr Lys Glu Val Lys Lys Glu Phe 
            260                 265                 270         


Glu Lys Arg Gly Cys Tyr Phe Leu Asn Glu Asp Glu Thr Glu Lys Val 
        275                 280                 285             


Arg Lys Thr Ile Ile Ile Asn Gly Ala Leu Asn Ala Lys Ile Val Gly 
    290                 295                 300                 


Gln Lys Ala His Thr Ile Ala Asn Leu Ala Gly Phe Glu Val Pro Glu 
305                 310                 315                 320 


Thr Thr Lys Ile Leu Ile Gly Glu Val Thr Ser Val Asp Ile Ser Glu 
                325                 330                 335     


Glu Phe Ala His Glu Lys Leu Cys Pro Val Leu Ala Met Tyr Arg Ala 
            340                 345                 350         


Lys Asp Phe Asp Asp Ala Leu Asp Lys Ala Glu Arg Leu Val Ala Asp 
        355                 360                 365             


Gly Gly Phe Gly His Thr Ser Ser Leu Tyr Ile Asp Thr Val Thr Gln 
    370                 375                 380                 


Lys Glu Lys Leu Gln Lys Phe Ser Glu Arg Met Lys Thr Cys Arg Ile 
385                 390                 395                 400 


Leu Val Asn Thr Pro Ser Ser Gln Gly Gly Ile Gly Asp Leu Tyr Asn 
                405                 410                 415     


Phe Lys Leu Ala Pro Ser Leu Thr Leu Gly Cys Gly Ser Trp Gly Gly 
            420                 425                 430         


Asn Ser Val Ser Asp Asn Val Gly Val Lys His Leu Leu Asn Ile Lys 
        435                 440                 445             


Thr Val Ala Glu Arg Arg Glu Asn Met Leu Trp Phe Arg Thr Pro Glu 
    450                 455                 460                 


Lys Ile Tyr Ile Lys Arg Gly Cys Leu Pro Val Ala Leu Asp Glu Leu 
465                 470                 475                 480 


Lys Asn Val Met Gly Lys Lys Lys Ala Phe Ile Val Thr Asp Asn Phe 
                485                 490                 495     


Leu Tyr Asn Asn Gly Tyr Thr Lys Pro Ile Thr Asp Lys Leu Asp Glu 
            500                 505                 510         


Met Gly Ile Val His Lys Thr Phe Phe Asp Val Ser Pro Asp Pro Ser 
        515                 520                 525             


Leu Ala Ser Ala Lys Ala Gly Ala Ala Glu Met Leu Ala Phe Gln Pro 
    530                 535                 540                 


Asp Thr Ile Ile Ala Val Gly Gly Arg Ser Ala Met Asp Ala Ala Lys 
545                 550                 555                 560 


Ile Met Trp Val Met Tyr Glu His Pro Glu Val Asp Phe Met Asp Met 
                565                 570                 575     


Ala Met Arg Phe Met Asp Ile Arg Lys Arg Val Tyr Thr Phe Pro Lys 
            580                 585                 590         


Met Gly Gln Lys Ala Tyr Phe Ile Ala Ile Pro Thr Ser Ala Gly Thr 
        595                 600                 605             


Gly Ser Glu Val Thr Pro Phe Ala Val Ile Thr Asp Glu Lys Thr Gly 
    610                 615                 620                 


Ile Lys Tyr Pro Leu Ala Asp Tyr Glu Leu Leu Pro Asp Met Ala Ile 
625                 630                 635                 640 


Val Asp Ala Asp Met Met Met Asn Ala Pro Lys Gly Leu Thr Ala Ala 
                645                 650                 655     


Ser Gly Ile Asp Ala Leu Thr His Ala Leu Glu Ala Tyr Val Ser Met 
            660                 665                 670         


Leu Ala Thr Asp Tyr Thr Asp Ser Leu Ala Leu Arg Ala Ile Lys Met 
        675                 680                 685             


Ile Phe Glu Tyr Leu Pro Arg Ala Tyr Glu Asn Gly Ala Ser Asp Pro 
    690                 695                 700                 


Val Ala Arg Glu Lys Met Ala Asn Ala Ala Thr Ile Ala Gly Met Ala 
705                 710                 715                 720 


Phe Ala Asn Ala Phe Leu Gly Val Cys His Ser Met Ala His Lys Leu 
                725                 730                 735     


Gly Ala Phe Tyr His Leu Pro His Gly Val Ala Asn Ala Leu Met Ile 
            740                 745                 750         


Asn Glu Val Ile Arg Phe Asn Ser Ser Glu Ala Pro Thr Lys Met Gly 
        755                 760                 765             


Thr Phe Pro Gln Tyr Asp His Pro Arg Thr Leu Glu Arg Tyr Ala Glu 
    770                 775                 780                 


Ile Ala Asp Tyr Ile Gly Leu Lys Gly Lys Asn Asn Glu Glu Lys Val 
785                 790                 795                 800 


Glu Asn Leu Ile Lys Ala Ile Asp Glu Leu Lys Glu Lys Val Gly Ile 
                805                 810                 815     


Arg Lys Thr Ile Lys Asp Tyr Asp Ile Asp Glu Lys Glu Phe Leu Asp 
            820                 825                 830         


Arg Leu Asp Glu Met Val Glu Gln Ala Phe Asp Asp Gln Cys Thr Gly 
        835                 840                 845             


Thr Asn Pro Arg Tyr Pro Leu Met Asn Glu Ile Arg Gln Met Tyr Leu 
    850                 855                 860                 


Asn Ala Tyr Tyr Gly Gly Ala Lys Lys 
865                 870             


<210>  20
<211>  2622
<212>  DNA
<213>  C.thermocellum bifunctional alcohol-acetaldehyde dehydrogenase

<400>  20
atgacgaaaa tagcgaataa atacgaagtt attgataatg ttgaaaagct tgaaaaggct       60

ttgaaacgtt taagagaagc tcaaagtgtt tatgcaacct atacacagga gcaggttgac      120

aaaattttct ttgaggcggc aatggcggcc aataaaatga gaattcctct tgccaaaatg      180

gctgtggagg aaacaggcat gggagtggtt gaagacaagg ttatcaaaaa ccactatgct      240

tctgagtata tctataatgc gtacaaaaac actaaaacct gcggtgttat tgaagaggac      300

cctgctttcg gtattaaaaa aatagcagag cctttggggg ttattgcggc ggttatacct      360

actacgaatc cgacatcgac agcaatcttt aagactctta ttgcattaaa gacgagaaat      420

gcaattatta taagcccaca ccccagggca aaaaactcaa cgatagaagc ggcgaaaatt      480

gttttggagg cggccgttaa agccggtgct ccggaaggca tcattggctg gattgatgtg      540

ccgagccttg aacttaccaa cctggtaatg agagaagcag atgtgattct cgcaacaggc      600

ggtcccggac tggttaaagc agcttactct tcgggcaaac cggctattgg tgtcggagcg      660

ggcaatactc ctgcaattat tgatgattcg gccgacattg tcttggcagt gaactcaata      720

atacattcaa aaactttcga caacggtatg atttgtgctt cagagcaatc ggtcattgtt      780

ctggacgggg tatataaaga ggtaaaaaaa gaatttgaaa aaagaggatg ctatttctta      840

aatgaagatg aaactgaaaa ggtaagaaaa acaattataa taaacggtgc gttaaatgcc      900

aagatagtag gtcagaaagc tcacacaatt gcaaaccttg caggttttga ggtacccgag      960

actacaaaaa ttctgatagg cgaagttacc agcgtggata tttccgaaga atttgcccac     1020

gaaaagttgt gcccggtact ggcaatgtac agggcaaagg attttgacga tgcccttgat     1080

aaagcagaaa ggttggtagc tgacggtgga tttggccata cttcgtcact ttatatagat     1140

acggtaacac agaaagagaa acttcagaaa ttctctgaaa ggatgaaaac ctgccgtata     1200

ttggttaata cgccgtcatc ccagggaggt atcggtgacc tttacaactt caagcttgct     1260

ccgtctctca ccctcggctg cggttcctgg ggaggaaatt cagtttccga caatgtggga     1320

gtcaagcatt tgttaaacat taaaacagtt gccgagagga gagagaacat gctctggttc     1380

agaacacctg aaaagattta tataaaaaga ggttgtctgc ctgttgcatt ggatgagctt     1440

aaaaatgtaa tgggtaaaaa gaaagcattt attgtaacgg gtaacttcct gtacaataac     1500

ggctacacca agccgattac ggataagctg gatgaaatgg gaattgtgca caagaccttc     1560

tttgatgtgt ctccagaccc atcccttgca tctgccaaag ccggtgcggc agaaatgctg     1620

gctttccagc ctgacaccat aattgcggtc ggcggcggat ctgccatgga cgcggccaaa     1680

atcatgtggg tgatgtatga acatccggaa gttgacttta tggacatggc aatgagattt     1740

atggatataa gaaagagagt ttacaccttc ccgaagatgg gacagaaggc atactttatc     1800

gcaattccga cttccgcggg tacaggttca gaagtgacac cttttgcggt tattactgat     1860

gaaaaaacag gaattaaata ccctctggcc gactatgaat tgttgccgga catggctatt     1920

gtagatgccg atatgatgat gaatgctcca aagggactta ccgcagcttc cggtatagac     1980

gcattgaccc acgctctgga agcctatgtt tcaatgcttg cgaccgacta tacggatagc     2040

cttgcccttc gtgcaataaa gatgatattt gaatatctcc cgagagccta tgaaaacggt     2100

gcaagtgacc cggttgcaag agagaaaatg gccaatgccg caacaatagc cggaatggct     2160

tttgccaatg cctttttggg tgtatgccat tcaatggcgc acaaactggg tgctttttat     2220

cacctgcccc acggtgttgc caatgcactt atgataaacg aagtaatcag attcaactca     2280

tccgaggctc cgaccaagat gggtactttc ccgcagtatg accatccgcg cacgctggaa     2340

aggtatgcag aaattgccga ttatatcgga cttaagggca agaataacga agaaaaagtt     2400

gaaaacttga ttaaagctat tgatgagctt aaagaaaagg tgggcatcag gaagaccatc     2460

aaagattatg acatagatga aaaggaattt ttggacagac tggacgaaat ggtggaacag     2520

gcttttgacg accagtgcac aggtacaaat ccaagatacc cgcttatgaa tgaaatcagg     2580

caaatgtatc tgaacgctta ttacggaggt gcgaagaaat ga                        2622


<210>  21
<211>  873
<212>  PRT
<213>  C.thermocellum bifunctional alcohol-acetaldehyde dehydrogenase

<400>  21

Met Thr Lys Ile Ala Asn Lys Tyr Glu Val Ile Asp Asn Val Glu Lys 
1               5                   10                  15      


Leu Glu Lys Ala Leu Lys Arg Leu Arg Glu Ala Gln Ser Val Tyr Ala 
            20                  25                  30          


Thr Tyr Thr Gln Glu Gln Val Asp Lys Ile Phe Phe Glu Ala Ala Met 
        35                  40                  45              


Ala Ala Asn Lys Met Arg Ile Pro Leu Ala Lys Met Ala Val Glu Glu 
    50                  55                  60                  


Thr Gly Met Gly Val Val Glu Asp Lys Val Ile Lys Asn His Tyr Ala 
65                  70                  75                  80  


Ser Glu Tyr Ile Tyr Asn Ala Tyr Lys Asn Thr Lys Thr Cys Gly Val 
                85                  90                  95      


Ile Glu Glu Asp Pro Ala Phe Gly Ile Lys Lys Ile Ala Glu Pro Leu 
            100                 105                 110         


Gly Val Ile Ala Ala Val Ile Pro Thr Thr Asn Pro Thr Ser Thr Ala 
        115                 120                 125             


Ile Phe Lys Thr Leu Ile Ala Leu Lys Thr Arg Asn Ala Ile Ile Ile 
    130                 135                 140                 


Ser Pro His Pro Arg Ala Lys Asn Ser Thr Ile Glu Ala Ala Lys Ile 
145                 150                 155                 160 


Val Leu Glu Ala Ala Val Lys Ala Gly Ala Pro Glu Gly Ile Ile Gly 
                165                 170                 175     


Trp Ile Asp Val Pro Ser Leu Glu Leu Thr Asn Leu Val Met Arg Glu 
            180                 185                 190         


Ala Asp Val Ile Leu Ala Thr Gly Gly Pro Gly Leu Val Lys Ala Ala 
        195                 200                 205             


Tyr Ser Ser Gly Lys Pro Ala Ile Gly Val Gly Ala Gly Asn Thr Pro 
    210                 215                 220                 


Ala Ile Ile Asp Asp Ser Ala Asp Ile Val Leu Ala Val Asn Ser Ile 
225                 230                 235                 240 


Ile His Ser Lys Thr Phe Asp Asn Gly Met Ile Cys Ala Ser Glu Gln 
                245                 250                 255     


Ser Val Ile Val Leu Asp Gly Val Tyr Lys Glu Val Lys Lys Glu Phe 
            260                 265                 270         


Glu Lys Arg Gly Cys Tyr Phe Leu Asn Glu Asp Glu Thr Glu Lys Val 
        275                 280                 285             


Arg Lys Thr Ile Ile Ile Asn Gly Ala Leu Asn Ala Lys Ile Val Gly 
    290                 295                 300                 


Gln Lys Ala His Thr Ile Ala Asn Leu Ala Gly Phe Glu Val Pro Glu 
305                 310                 315                 320 


Thr Thr Lys Ile Leu Ile Gly Glu Val Thr Ser Val Asp Ile Ser Glu 
                325                 330                 335     


Glu Phe Ala His Glu Lys Leu Cys Pro Val Leu Ala Met Tyr Arg Ala 
            340                 345                 350         


Lys Asp Phe Asp Asp Ala Leu Asp Lys Ala Glu Arg Leu Val Ala Asp 
        355                 360                 365             


Gly Gly Phe Gly His Thr Ser Ser Leu Tyr Ile Asp Thr Val Thr Gln 
    370                 375                 380                 


Lys Glu Lys Leu Gln Lys Phe Ser Glu Arg Met Lys Thr Cys Arg Ile 
385                 390                 395                 400 


Leu Val Asn Thr Pro Ser Ser Gln Gly Gly Ile Gly Asp Leu Tyr Asn 
                405                 410                 415     


Phe Lys Leu Ala Pro Ser Leu Thr Leu Gly Cys Gly Ser Trp Gly Gly 
            420                 425                 430         


Asn Ser Val Ser Asp Asn Val Gly Val Lys His Leu Leu Asn Ile Lys 
        435                 440                 445             


Thr Val Ala Glu Arg Arg Glu Asn Met Leu Trp Phe Arg Thr Pro Glu 
    450                 455                 460                 


Lys Ile Tyr Ile Lys Arg Gly Cys Leu Pro Val Ala Leu Asp Glu Leu 
465                 470                 475                 480 


Lys Asn Val Met Gly Lys Lys Lys Ala Phe Ile Val Thr Gly Asn Phe 
                485                 490                 495     


Leu Tyr Asn Asn Gly Tyr Thr Lys Pro Ile Thr Asp Lys Leu Asp Glu 
            500                 505                 510         


Met Gly Ile Val His Lys Thr Phe Phe Asp Val Ser Pro Asp Pro Ser 
        515                 520                 525             


Leu Ala Ser Ala Lys Ala Gly Ala Ala Glu Met Leu Ala Phe Gln Pro 
    530                 535                 540                 


Asp Thr Ile Ile Ala Val Gly Gly Gly Ser Ala Met Asp Ala Ala Lys 
545                 550                 555                 560 


Ile Met Trp Val Met Tyr Glu His Pro Glu Val Asp Phe Met Asp Met 
                565                 570                 575     


Ala Met Arg Phe Met Asp Ile Arg Lys Arg Val Tyr Thr Phe Pro Lys 
            580                 585                 590         


Met Gly Gln Lys Ala Tyr Phe Ile Ala Ile Pro Thr Ser Ala Gly Thr 
        595                 600                 605             


Gly Ser Glu Val Thr Pro Phe Ala Val Ile Thr Asp Glu Lys Thr Gly 
    610                 615                 620                 


Ile Lys Tyr Pro Leu Ala Asp Tyr Glu Leu Leu Pro Asp Met Ala Ile 
625                 630                 635                 640 


Val Asp Ala Asp Met Met Met Asn Ala Pro Lys Gly Leu Thr Ala Ala 
                645                 650                 655     


Ser Gly Ile Asp Ala Leu Thr His Ala Leu Glu Ala Tyr Val Ser Met 
            660                 665                 670         


Leu Ala Thr Asp Tyr Thr Asp Ser Leu Ala Leu Arg Ala Ile Lys Met 
        675                 680                 685             


Ile Phe Glu Tyr Leu Pro Arg Ala Tyr Glu Asn Gly Ala Ser Asp Pro 
    690                 695                 700                 


Val Ala Arg Glu Lys Met Ala Asn Ala Ala Thr Ile Ala Gly Met Ala 
705                 710                 715                 720 


Phe Ala Asn Ala Phe Leu Gly Val Cys His Ser Met Ala His Lys Leu 
                725                 730                 735     


Gly Ala Phe Tyr His Leu Pro His Gly Val Ala Asn Ala Leu Met Ile 
            740                 745                 750         


Asn Glu Val Ile Arg Phe Asn Ser Ser Glu Ala Pro Thr Lys Met Gly 
        755                 760                 765             


Thr Phe Pro Gln Tyr Asp His Pro Arg Thr Leu Glu Arg Tyr Ala Glu 
    770                 775                 780                 


Ile Ala Asp Tyr Ile Gly Leu Lys Gly Lys Asn Asn Glu Glu Lys Val 
785                 790                 795                 800 


Glu Asn Leu Ile Lys Ala Ile Asp Glu Leu Lys Glu Lys Val Gly Ile 
                805                 810                 815     


Arg Lys Thr Ile Lys Asp Tyr Asp Ile Asp Glu Lys Glu Phe Leu Asp 
            820                 825                 830         


Arg Leu Asp Glu Met Val Glu Gln Ala Phe Asp Asp Gln Cys Thr Gly 
        835                 840                 845             


Thr Asn Pro Arg Tyr Pro Leu Met Asn Glu Ile Arg Gln Met Tyr Leu 
    850                 855                 860                 


Asn Ala Tyr Tyr Gly Gly Ala Lys Lys 
865                 870             


<210>  22
<211>  957
<212>  DNA
<213>  C.thermocellum malate dehydrogenase

<400>  22
atggaaatgg taaaaagtag gtcaaaagtt gcaatcattg gtgctggttt tgtaggtgcg       60

tctgcagcct tcacaatggc tttgcggcaa accgcaaatg aacttgttct catcgatgtt      120

ttcaaggaaa aagccatagg cgaggctatg gatattaacc acggtcttcc atttatggga      180

cagatgtcat tgtatgccgg tgattattcc gacgttaaag actgtgatgt tatcgtagtc      240

acggccggag ccaacagaaa acctggtgaa acacgtcttg accttgcaaa gaaaaacgtt      300

atgattgcaa aagaagtaac tcaaaacatc atgaagtatt acaaccatgg tgtaatactt      360

gtagtatcca atcctgttga cattataact tatatgatcc aaaaatggtc aggcctccct      420

gtgggaaaag ttataggttc aggtaccgta cttgacagta tcagattcag atacttgtta      480

agcgaaaaat tgggcgttga cgtaaagaat gtacacggct acataatagg cgaacacggt      540

gattcacagc ttccgttgtg gagctgcaca catatcgccg gtaaaaatat caacgaatat      600

atcgatgatc cgaaatgcaa tttcacagaa gaagacaaga aaaaaatcgc tgaagatgtt      660

aaaactgcgg gtgcaaccat tatcaagaac aaaggtgcaa catactatgg tattgcagtt      720

tcaatcaaca caatagttga aacactcctt aagaatcaga atacaataag aaccgtagga      780

accgttataa acggcatgta tggaatagaa gatgttgcaa taagccttcc atccatcgta      840

aattccgaag gtgttcagga agttctccaa tttaatctga ctcctgaaga agaagaagct      900

ttaagattct cagcggagca ggttaaaaaa gtattgaacg aagttaagaa tttataa         957


<210>  23
<211>  318
<212>  PRT
<213>  C.thermocellum malate dehydrogenase

<400>  23

Met Glu Met Val Lys Ser Arg Ser Lys Val Ala Ile Ile Gly Ala Gly 
1               5                   10                  15      


Phe Val Gly Ala Ser Ala Ala Phe Thr Met Ala Leu Arg Gln Thr Ala 
            20                  25                  30          


Asn Glu Leu Val Leu Ile Asp Val Phe Lys Glu Lys Ala Ile Gly Glu 
        35                  40                  45              


Ala Met Asp Ile Asn His Gly Leu Pro Phe Met Gly Gln Met Ser Leu 
    50                  55                  60                  


Tyr Ala Gly Asp Tyr Ser Asp Val Lys Asp Cys Asp Val Ile Val Val 
65                  70                  75                  80  


Thr Ala Gly Ala Asn Arg Lys Pro Gly Glu Thr Arg Leu Asp Leu Ala 
                85                  90                  95      


Lys Lys Asn Val Met Ile Ala Lys Glu Val Thr Gln Asn Ile Met Lys 
            100                 105                 110         


Tyr Tyr Asn His Gly Val Ile Leu Val Val Ser Asn Pro Val Asp Ile 
        115                 120                 125             


Ile Thr Tyr Met Ile Gln Lys Trp Ser Gly Leu Pro Val Gly Lys Val 
    130                 135                 140                 


Ile Gly Ser Gly Thr Val Leu Asp Ser Ile Arg Phe Arg Tyr Leu Leu 
145                 150                 155                 160 


Ser Glu Lys Leu Gly Val Asp Val Lys Asn Val His Gly Tyr Ile Ile 
                165                 170                 175     


Gly Glu His Gly Asp Ser Gln Leu Pro Leu Trp Ser Cys Thr His Ile 
            180                 185                 190         


Ala Gly Lys Asn Ile Asn Glu Tyr Ile Asp Asp Pro Lys Cys Asn Phe 
        195                 200                 205             


Thr Glu Glu Asp Lys Lys Lys Ile Ala Glu Asp Val Lys Thr Ala Gly 
    210                 215                 220                 


Ala Thr Ile Ile Lys Asn Lys Gly Ala Thr Tyr Tyr Gly Ile Ala Val 
225                 230                 235                 240 


Ser Ile Asn Thr Ile Val Glu Thr Leu Leu Lys Asn Gln Asn Thr Ile 
                245                 250                 255     


Arg Thr Val Gly Thr Val Ile Asn Gly Met Tyr Gly Ile Glu Asp Val 
            260                 265                 270         


Ala Ile Ser Leu Pro Ser Ile Val Asn Ser Glu Gly Val Gln Glu Val 
        275                 280                 285             


Leu Gln Phe Asn Leu Thr Pro Glu Glu Glu Glu Ala Leu Arg Phe Ser 
    290                 295                 300                 


Ala Glu Gln Val Lys Lys Val Leu Asn Glu Val Lys Asn Leu 
305                 310                 315             


<210>  24
<211>  942
<212>  DNA
<213>  Methanocaldococcus jannaschii malate dehydrogenase

<400>  24
atgaaagtta caattatagg agcttctggt agagttggga gtgcaacagc cttattatta       60

gctaaagaac cttttatgaa ggatttggtg ttaattggaa gagaacattc aataaataaa      120

ttggaaggat tgagagaaga catctacgat gccttagctg ggacaagaag tgatgcaaat      180

atatacgttg agagtgatga aaatctaagg ataattgatg aaagtgatgt tgttataata      240

acaagcggtg ttccaagaaa agagggaatg agtaggatgg atttggcaaa aacaaatgca      300

aaaattgttg ggaagtatgc taaaaaaata gctgaaatct gcgatacaaa aatatttgtt      360

ataacaaacc ctgtggatgt gatgacttat aaagctctgg tagattcaaa atttgaaaga      420

aatcaagttt ttggattagg gactcattta gattctttga ggtttaaggt tgctattgct      480

aagtttttcg gtgttcatat tgatgaagtt aggacgagaa ttattggaga gcatggggac      540

agcatggttc cattgttaag tgctacctct atcggaggaa ttcctattca aaaatttgaa      600

agatttaagg aactgccaat agatgagatt atagaggatg ttaaaacaaa aggagagcag      660

attattagat tgaaaggagg ttctgagttt ggtccagcag cagccatttt aaatgttgtt      720

aggtgtattg tgaataatga gaaaagattg ctaactttat ccgcttacgt agatggagag      780

tttgatggaa ttagagatgt gtgtattgga gttccagtaa agattggaag agatgggata      840

gaagaggttg tatcaattga attggataaa gatgagataa ttgcatttag aaaatctgct      900

gaaatcatta aaaaatactg tgaagaagtt aaaaacttat aa                         942


<210>  25
<211>  313
<212>  PRT
<213>  Methanocaldococcus jannaschii malate dehydrogenase

<400>  25

Met Lys Val Thr Ile Ile Gly Ala Ser Gly Arg Val Gly Ser Ala Thr 
1               5                   10                  15      


Ala Leu Leu Leu Ala Lys Glu Pro Phe Met Lys Asp Leu Val Leu Ile 
            20                  25                  30          


Gly Arg Glu His Ser Ile Asn Lys Leu Glu Gly Leu Arg Glu Asp Ile 
        35                  40                  45              


Tyr Asp Ala Leu Ala Gly Thr Arg Ser Asp Ala Asn Ile Tyr Val Glu 
    50                  55                  60                  


Ser Asp Glu Asn Leu Arg Ile Ile Asp Glu Ser Asp Val Val Ile Ile 
65                  70                  75                  80  


Thr Ser Gly Val Pro Arg Lys Glu Gly Met Ser Arg Met Asp Leu Ala 
                85                  90                  95      


Lys Thr Asn Ala Lys Ile Val Gly Lys Tyr Ala Lys Lys Ile Ala Glu 
            100                 105                 110         


Ile Cys Asp Thr Lys Ile Phe Val Ile Thr Asn Pro Val Asp Val Met 
        115                 120                 125             


Thr Tyr Lys Ala Leu Val Asp Ser Lys Phe Glu Arg Asn Gln Val Phe 
    130                 135                 140                 


Gly Leu Gly Thr His Leu Asp Ser Leu Arg Phe Lys Val Ala Ile Ala 
145                 150                 155                 160 


Lys Phe Phe Gly Val His Ile Asp Glu Val Arg Thr Arg Ile Ile Gly 
                165                 170                 175     


Glu His Gly Asp Ser Met Val Pro Leu Leu Ser Ala Thr Ser Ile Gly 
            180                 185                 190         


Gly Ile Pro Ile Gln Lys Phe Glu Arg Phe Lys Glu Leu Pro Ile Asp 
        195                 200                 205             


Glu Ile Ile Glu Asp Val Lys Thr Lys Gly Glu Gln Ile Ile Arg Leu 
    210                 215                 220                 


Lys Gly Gly Ser Glu Phe Gly Pro Ala Ala Ala Ile Leu Asn Val Val 
225                 230                 235                 240 


Arg Cys Ile Val Asn Asn Glu Lys Arg Leu Leu Thr Leu Ser Ala Tyr 
                245                 250                 255     


Val Asp Gly Glu Phe Asp Gly Ile Arg Asp Val Cys Ile Gly Val Pro 
            260                 265                 270         


Val Lys Ile Gly Arg Asp Gly Ile Glu Glu Val Val Ser Ile Glu Leu 
        275                 280                 285             


Asp Lys Asp Glu Ile Ile Ala Phe Arg Lys Ser Ala Glu Ile Ile Lys 
    290                 295                 300                 


Lys Tyr Cys Glu Glu Val Lys Asn Leu 
305                 310             


<210>  26
<211>  1203
<212>  DNA
<213>  Pseudomonas sp. formate dehydrogenase (NAD+) codon optimized

<400>  26
atggcaaaag tattatgtgt attatatgat gatccagtag atggatatcc aaaaacatat       60

gcaagagatg atttaccaaa aatagatcat tatccaggag gacaaacatt accaacacca      120

aaagcaatag attttacacc aggacaatta ttaggatcag tatcaggaga attaggatta      180

agaaaatatt tagaatcaaa tggacataca ttagtagtaa catcagataa agatggacca      240

gattcagtat ttgaaagaga attagtagat gcagatgtag taatatcaca accattttgg      300

ccagcatatt taacaccaga aagaatagca aaagcaaaaa atttaaaatt agcattaaca      360

gcaggaatag gatcagatca tgtagattta caatcagcaa tagatagaaa tgtaacagta      420

gcagaagtaa catattgtaa ttcaatatca gtagcagaac atgtagtaat gatgatatta      480

tcattagtaa gaaattattt accatcacat gaatgggcaa gaaaaggagg atggaatata      540

gcagattgtg tatcacatgc atatgattta gaagcaatgc atgtaggaac agtagcagca      600

ggaagaatag gattagcagt attaagaaga ttagcaccat ttgatgtaca tttacattat      660

acagatagac atagattacc agaatcagta gaaaaagaat taaatttaac atggcatgca      720

acaagagaag atatgtatcc agtatgtgat gtagtaacat taaattgtcc attacatcca      780

gaaacagaac atatgataaa tgatgaaaca ttaaaattat ttaaaagagg agcatatata      840

gtaaatacag caagaggaaa attatgtgat agagatgcag tagcaagagc attagaatca      900

ggaagattag caggatatgc aggagatgta tggtttccac aaccagcacc aaaagatcat      960

ccatggagaa caatgccata taatggaatg acaccacata tatcaggaac aacattaaca     1020

gcacaagcaa gatatgcagc aggaacaaga gaaatattag aatgtttttt tgaaggaaga     1080

ccaataagag atgaatattt aatagtacaa ggaggagcat tagcaggaac aggagcacat     1140

tcatattcaa aaggaaatgc aacaggagga tcagaagaag cagcaaaatt taaaaaagca     1200

gta                                                                   1203


<210>  27
<211>  401
<212>  PRT
<213>  Pseudomonas sp. formate dehydrogenase (NAD+)

<400>  27

Met Ala Lys Val Leu Cys Val Leu Tyr Asp Asp Pro Val Asp Gly Tyr 
1               5                   10                  15      


Pro Lys Thr Tyr Ala Arg Asp Asp Leu Pro Lys Ile Asp His Tyr Pro 
            20                  25                  30          


Gly Gly Gln Thr Leu Pro Thr Pro Lys Ala Ile Asp Phe Thr Pro Gly 
        35                  40                  45              


Gln Leu Leu Gly Ser Val Ser Gly Glu Leu Gly Leu Arg Lys Tyr Leu 
    50                  55                  60                  


Glu Ser Asn Gly His Thr Leu Val Val Thr Ser Asp Lys Asp Gly Pro 
65                  70                  75                  80  


Asp Ser Val Phe Glu Arg Glu Leu Val Asp Ala Asp Val Val Ile Ser 
                85                  90                  95      


Gln Pro Phe Trp Pro Ala Tyr Leu Thr Pro Glu Arg Ile Ala Lys Ala 
            100                 105                 110         


Lys Asn Leu Lys Leu Ala Leu Thr Ala Gly Ile Gly Ser Asp His Val 
        115                 120                 125             


Asp Leu Gln Ser Ala Ile Asp Arg Asn Val Thr Val Ala Glu Val Thr 
    130                 135                 140                 


Tyr Cys Asn Ser Ile Ser Val Ala Glu His Val Val Met Met Ile Leu 
145                 150                 155                 160 


Ser Leu Val Arg Asn Tyr Leu Pro Ser His Glu Trp Ala Arg Lys Gly 
                165                 170                 175     


Gly Trp Asn Ile Ala Asp Cys Val Ser His Ala Tyr Asp Leu Glu Ala 
            180                 185                 190         


Met His Val Gly Thr Val Ala Ala Gly Arg Ile Gly Leu Ala Val Leu 
        195                 200                 205             


Arg Arg Leu Ala Pro Phe Asp Val His Leu His Tyr Thr Asp Arg His 
    210                 215                 220                 


Arg Leu Pro Glu Ser Val Glu Lys Glu Leu Asn Leu Thr Trp His Ala 
225                 230                 235                 240 


Thr Arg Glu Asp Met Tyr Pro Val Cys Asp Val Val Thr Leu Asn Cys 
                245                 250                 255     


Pro Leu His Pro Glu Thr Glu His Met Ile Asn Asp Glu Thr Leu Lys 
            260                 265                 270         


Leu Phe Lys Arg Gly Ala Tyr Ile Val Asn Thr Ala Arg Gly Lys Leu 
        275                 280                 285             


Cys Asp Arg Asp Ala Val Ala Arg Ala Leu Glu Ser Gly Arg Leu Ala 
    290                 295                 300                 


Gly Tyr Ala Gly Asp Val Trp Phe Pro Gln Pro Ala Pro Lys Asp His 
305                 310                 315                 320 


Pro Trp Arg Thr Met Pro Tyr Asn Gly Met Thr Pro His Ile Ser Gly 
                325                 330                 335     


Thr Thr Leu Thr Ala Gln Ala Arg Tyr Ala Ala Gly Thr Arg Glu Ile 
            340                 345                 350         


Leu Glu Cys Phe Phe Glu Gly Arg Pro Ile Arg Asp Glu Tyr Leu Ile 
        355                 360                 365             


Val Gln Gly Gly Ala Leu Ala Gly Thr Gly Ala His Ser Tyr Ser Lys 
    370                 375                 380                 


Gly Asn Ala Thr Gly Gly Ser Glu Glu Ala Ala Lys Phe Lys Lys Ala 
385                 390                 395                 400 


Val 
    


<210>  28
<211>  2700
<212>  DNA
<213>  Moorella thermoaceticum formate dehydrogenase (NAD+) alpha subunit

<400>  28
atggttaacc tgaccattga cggacaaagg gttacggctc cagagggcat gaccatcctg       60

gaggtggccc gggaaaatgg tatccatatc cccaccctgt gccaccatcc aaagttgcgg      120

cccctggggt attgccgtct gtgcctggtc gacatcgagg gcgccgcaaa acccatgacg      180

gcctgcaata cgccggtcgc cgagggtatg gtgatccgga ccagcacgcc tgttatagag      240

gagatgcgca agggtattat tgaaatgctt ttaagcctcc acccggagga ctgcctgacc      300

tgcgaaaaag caggtaactg ccagctccag gattgcgcct acacttacgg tgtaaagcat      360

ggcgaactgc cagtaaaacg cgaggaattg cccgttttga aggaaaatcc cttcattgtg      420

cgggattata ataaatgtat cgtttgcggc cgttgcgtcc gcgcctgcca ggaggtgcag      480

gtccagaggg tcgtggacct ggtgggtaaa ggcagcgccg cccgggtggg ggcgacgaag      540

gccggagcgg aagtaagcct ggaagaaggg ggctgcgtct tttgcggtaa ctgtgtccag      600

gtctgcccgg tgggagctct gacggagaag gccggcctgg gccagggccg cgagtgggag      660

ttcaaaaaag tccgcagtat ttgttcttac tgcggcgtgg gttgtaatct caccctttat      720

gtaaaagatg gtaaggtggt aaaagttagg ggttacgaaa accctgaggt aaacaacggc      780

tggctgtgcg taaaaggccg ctttggtttt gactatattc acaatcctga caggataacc      840

aggccgttga tccgggaggg agatagggaa aaaggctatt tccgggaggc ttcctgggaa      900

gaagctttag cccttgtatc ccagaaatta actcagatta aaggcagcta cggctctgaa      960

gctctgggct ttctttgttc agctaaatgt accaatgaag agaattatct cttacaaaag     1020

ctggcccggg gggtactggg caccaataat gttgatcact gtgctcgcct ccactcttct     1080

acagttgccg gtctggcaac aacctttggc agcggtgcaa tgaccaattc tatcgctgac     1140

atcgccagcg cagattgtat ctttgtcatt ggcagcaata caaccgagaa ccatcctgtt     1200

attgccctta aagtaaaaga agctgtccgt cgtggagcca ggctcattgt tgctgatccc     1260

cggcgtattg aactggtgaa cttcagttac ttgtggttaa gacaaaaacc cggaacagat     1320

cttgctctgc tgaatggact gcttcatgta atcatcaagg aagagcttta tgacaaagaa     1380

tttattgccc agaggacgga aggttttgag gctctaaaac ttgccgtaga ggagtataca     1440

ccagcaaagg tgtcagaagt tacaggtgtt ccggcaggcg atattatcga ggcagcaagg     1500

acttatgccc ggggtccgag ctctactatt ttgtacgcaa tgggaataac ccagcatata     1560

actggtacgg ccaacgtgat ggccctggcc aacctggcca tggcctgtgg tcaggtcggt     1620

aaagaaggta gcggcgtaaa tcccctgcgg gggcagagca atgtccaggg tgcctgcgat     1680

atgggtggat tacccaatgt attaccggga taccaaccag taacagatcc gggggttcgc     1740

cataaattta gcgaagcctg gggggtaccg gacttacccg gagaacctgg cctgacatta     1800

atggagatga tggcggcagc ccaagaaggc aaattgaaag ggatgtatat tttaggagaa     1860

aaccctgtct tgactgatcc agatgtctcc catgtaaaag aggcgttaaa gaacctggag     1920

tttctggtgg tacaggatat ttttttgacg gagacagcca ggatggcgga tgttgtttta     1980

cctggagctt cctttgcgga aaaggaaggt acctttacca gtacggagcg ccgggtgcag     2040

cttttgcata aagccattga acctcccggt gaagcacggc cggattggct tattttaaac     2100

gatttgttgc tgttaatggg atatccgcgg aaatattcgt cgcctgggga gataatgcag     2160

gagatagcag ggttaactcc cagctatgcg ggtataactt atgagcgcct ggaagataaa     2220

gggttacagt ggccggtgct ttccctcgaa catccgggta cacccgttct ccatcgggaa     2280

aaatttagca gaggttatgg gcagttccag gtagtgcatt accggccgcc ggccgaagaa     2340

cctgatgagg agtacccgtt cttatttacc actggcagga atttgtatca ctatcatact     2400

gttatttccc gtaagtccag ggggctggaa gagatgtgtc ctgctcctgt ggtggagatt     2460

aatgataacg atgcagcccg tttgggtata cgggaaggag aaatgattga gattgtttcc     2520

cgacgtggta aagtaagggt taaagcattg gttacggatc gcataccccg gggccaggta     2580

tttatgaatt tccatttcca tgaagcagca gccaacctgc ttacaattgc tgccctggat     2640

ccggttgcta aaatacccga gtataaaacc tgtgctgtag ctatcaaggt taaaaagtag     2700


<210>  29
<211>  899
<212>  PRT
<213>  Moorella thermoaceticum formate dehydrogenase (NAD+) alpha subunit

<400>  29

Met Val Asn Leu Thr Ile Asp Gly Gln Arg Val Thr Ala Pro Glu Gly 
1               5                   10                  15      


Met Thr Ile Leu Glu Val Ala Arg Glu Asn Gly Ile His Ile Pro Thr 
            20                  25                  30          


Leu Cys His His Pro Lys Leu Arg Pro Leu Gly Tyr Cys Arg Leu Cys 
        35                  40                  45              


Leu Val Asp Ile Glu Gly Ala Ala Lys Pro Met Thr Ala Cys Asn Thr 
    50                  55                  60                  


Pro Val Ala Glu Gly Met Val Ile Arg Thr Ser Thr Pro Val Ile Glu 
65                  70                  75                  80  


Glu Met Arg Lys Gly Ile Ile Glu Met Leu Leu Ser Leu His Pro Glu 
                85                  90                  95      


Asp Cys Leu Thr Cys Glu Lys Ala Gly Asn Cys Gln Leu Gln Asp Cys 
            100                 105                 110         


Ala Tyr Thr Tyr Gly Val Lys His Gly Glu Leu Pro Val Lys Arg Glu 
        115                 120                 125             


Glu Leu Pro Val Leu Lys Glu Asn Pro Phe Ile Val Arg Asp Tyr Asn 
    130                 135                 140                 


Lys Cys Ile Val Cys Gly Arg Cys Val Arg Ala Cys Gln Glu Val Gln 
145                 150                 155                 160 


Val Gln Arg Val Val Asp Leu Val Gly Lys Gly Ser Ala Ala Arg Val 
                165                 170                 175     


Gly Ala Thr Lys Ala Gly Ala Glu Val Ser Leu Glu Glu Gly Gly Cys 
            180                 185                 190         


Val Phe Cys Gly Asn Cys Val Gln Val Cys Pro Val Gly Ala Leu Thr 
        195                 200                 205             


Glu Lys Ala Gly Leu Gly Gln Gly Arg Glu Trp Glu Phe Lys Lys Val 
    210                 215                 220                 


Arg Ser Ile Cys Ser Tyr Cys Gly Val Gly Cys Asn Leu Thr Leu Tyr 
225                 230                 235                 240 


Val Lys Asp Gly Lys Val Val Lys Val Arg Gly Tyr Glu Asn Pro Glu 
                245                 250                 255     


Val Asn Asn Gly Trp Leu Cys Val Lys Gly Arg Phe Gly Phe Asp Tyr 
            260                 265                 270         


Ile His Asn Pro Asp Arg Ile Thr Arg Pro Leu Ile Arg Glu Gly Asp 
        275                 280                 285             


Arg Glu Lys Gly Tyr Phe Arg Glu Ala Ser Trp Glu Glu Ala Leu Ala 
    290                 295                 300                 


Leu Val Ser Gln Lys Leu Thr Gln Ile Lys Gly Ser Tyr Gly Ser Glu 
305                 310                 315                 320 


Ala Leu Gly Phe Leu Cys Ser Ala Lys Cys Thr Asn Glu Glu Asn Tyr 
                325                 330                 335     


Leu Leu Gln Lys Leu Ala Arg Gly Val Leu Gly Thr Asn Asn Val Asp 
            340                 345                 350         


His Cys Ala Arg Leu His Ser Ser Thr Val Ala Gly Leu Ala Thr Thr 
        355                 360                 365             


Phe Gly Ser Gly Ala Met Thr Asn Ser Ile Ala Asp Ile Ala Ser Ala 
    370                 375                 380                 


Asp Cys Ile Phe Val Ile Gly Ser Asn Thr Thr Glu Asn His Pro Val 
385                 390                 395                 400 


Ile Ala Leu Lys Val Lys Glu Ala Val Arg Arg Gly Ala Arg Leu Ile 
                405                 410                 415     


Val Ala Asp Pro Arg Arg Ile Glu Leu Val Asn Phe Ser Tyr Leu Trp 
            420                 425                 430         


Leu Arg Gln Lys Pro Gly Thr Asp Leu Ala Leu Leu Asn Gly Leu Leu 
        435                 440                 445             


His Val Ile Ile Lys Glu Glu Leu Tyr Asp Lys Glu Phe Ile Ala Gln 
    450                 455                 460                 


Arg Thr Glu Gly Phe Glu Ala Leu Lys Leu Ala Val Glu Glu Tyr Thr 
465                 470                 475                 480 


Pro Ala Lys Val Ser Glu Val Thr Gly Val Pro Ala Gly Asp Ile Ile 
                485                 490                 495     


Glu Ala Ala Arg Thr Tyr Ala Arg Gly Pro Ser Ser Thr Ile Leu Tyr 
            500                 505                 510         


Ala Met Gly Ile Thr Gln His Ile Thr Gly Thr Ala Asn Val Met Ala 
        515                 520                 525             


Leu Ala Asn Leu Ala Met Ala Cys Gly Gln Val Gly Lys Glu Gly Ser 
    530                 535                 540                 


Gly Val Asn Pro Leu Arg Gly Gln Ser Asn Val Gln Gly Ala Cys Asp 
545                 550                 555                 560 


Met Gly Gly Leu Pro Asn Val Leu Pro Gly Tyr Gln Pro Val Thr Asp 
                565                 570                 575     


Pro Gly Val Arg His Lys Phe Ser Glu Ala Trp Gly Val Pro Asp Leu 
            580                 585                 590         


Pro Gly Glu Pro Gly Leu Thr Leu Met Glu Met Met Ala Ala Ala Gln 
        595                 600                 605             


Glu Gly Lys Leu Lys Gly Met Tyr Ile Leu Gly Glu Asn Pro Val Leu 
    610                 615                 620                 


Thr Asp Pro Asp Val Ser His Val Lys Glu Ala Leu Lys Asn Leu Glu 
625                 630                 635                 640 


Phe Leu Val Val Gln Asp Ile Phe Leu Thr Glu Thr Ala Arg Met Ala 
                645                 650                 655     


Asp Val Val Leu Pro Gly Ala Ser Phe Ala Glu Lys Glu Gly Thr Phe 
            660                 665                 670         


Thr Ser Thr Glu Arg Arg Val Gln Leu Leu His Lys Ala Ile Glu Pro 
        675                 680                 685             


Pro Gly Glu Ala Arg Pro Asp Trp Leu Ile Leu Asn Asp Leu Leu Leu 
    690                 695                 700                 


Leu Met Gly Tyr Pro Arg Lys Tyr Ser Ser Pro Gly Glu Ile Met Gln 
705                 710                 715                 720 


Glu Ile Ala Gly Leu Thr Pro Ser Tyr Ala Gly Ile Thr Tyr Glu Arg 
                725                 730                 735     


Leu Glu Asp Lys Gly Leu Gln Trp Pro Val Leu Ser Leu Glu His Pro 
            740                 745                 750         


Gly Thr Pro Val Leu His Arg Glu Lys Phe Ser Arg Gly Tyr Gly Gln 
        755                 760                 765             


Phe Gln Val Val His Tyr Arg Pro Pro Ala Glu Glu Pro Asp Glu Glu 
    770                 775                 780                 


Tyr Pro Phe Leu Phe Thr Thr Gly Arg Asn Leu Tyr His Tyr His Thr 
785                 790                 795                 800 


Val Ile Ser Arg Lys Ser Arg Gly Leu Glu Glu Met Cys Pro Ala Pro 
                805                 810                 815     


Val Val Glu Ile Asn Asp Asn Asp Ala Ala Arg Leu Gly Ile Arg Glu 
            820                 825                 830         


Gly Glu Met Ile Glu Ile Val Ser Arg Arg Gly Lys Val Arg Val Lys 
        835                 840                 845             


Ala Leu Val Thr Asp Arg Ile Pro Arg Gly Gln Val Phe Met Asn Phe 
    850                 855                 860                 


His Phe His Glu Ala Ala Ala Asn Leu Leu Thr Ile Ala Ala Leu Asp 
865                 870                 875                 880 


Pro Val Ala Lys Ile Pro Glu Tyr Lys Thr Cys Ala Val Ala Ile Lys 
                885                 890                 895     


Val Lys Lys 
            


<210>  30
<211>  2124
<212>  DNA
<213>  Moorella thermoaceticum formate dehydrogenase (NAD+) beta subunit

<400>  30
ttgggagagg tggtatttag cacctgggga ggaaaagttg tcgatcaccg gggcggtccg       60

tcggggggag gaccgtcctg ggccggggaa tttggcggtc ggcagttaaa ggcctttatt      120

ggctgggatg gtctcgtagt caccgacccg gccgtagacc tgctggctgc tttacaggct      180

tactaccagg ccgtccaggg ggaatcctgc ggccgctgcg taccctgccg ggtagggacc      240

agggttattt acaatgtcct ggtacgcatc gccgggggcg aaggccttcc ctccgattta      300

gaccttctcc gacgggtggc ctggattgta cgggatggtt ctctgtgcga gctcggccag      360

gctggggcca aagctgttct tgatttttta gattattata gcgaggcctt gcgacctttc      420

ctggaggata gcggcagggt cgccgggggt caacggagac cagggcctgg aggccgggtg      480

caggttctgg cttccggacg ggtcctggtg gggaacgacc gcggaaaggg agcggccgcc      540

gcttccccgg cagccggttt aacctataaa ccttttgtta ctgcgccctg cctcaagcgg      600

tgcccggccc acctggacat cccggcttat attgacgcca ttaaggatgg ccgctatgag      660

gaatccctgg ccatcatccg ccagcggacc gccctggccg gtgtcctggg acgggtctgc      720

gtccacccgt gtgaagaaaa ctgccgccgc ggcaatgttg acgaacccct ggccatccgg      780

ggcctgaagc gcttcgtagc cgattatgag gtgaaacgcg gccgccggcc ggtcgccgtc      840

tgcgggggta atctctttac gggaccctgg cggccggcag gacaggccgg tggggaagaa      900

acaacggccg tcacttcagg caagaaggta gccatcatcg gtgccggacc ggcgggtctc      960

agcgccgcct accagctcgc cggtcggggc tataaagtga ctattttcga ggccttgccg     1020

gtggccggcg gtatgctggc ggtgggtatt cccagctacc ggttgccacg ggatatcctg     1080

gctggagaga tcgaggccat caaggctctg ggcgtaacca tcaacctcaa cacccgggtc     1140

ggcgtcgacg tgaccatgga ccaattacag cgcgattacg acgccgtctt tattgctacc     1200

ggcctccatg ccagctcccg catgggagta gccggcgagg atgaaggcta tggaggattt     1260

atccccgggg tcaagttcct gcgagatttg aacctggacc ggtgtccttc cctggagggc     1320

aaggtggtgg ccgtcgtcgg cggcggcaat gtagcgatgg attgcgcccg ttccgccctg     1380

cgccgggggg cccgggaggt gcaccttatt taccggcgtt cccgggcgga aatgccggcc     1440

catgcgaccg aggtcaggga tgccgaggcc gagggggtta tttatcactt cctggttaat     1500

cccaccgccc tggtagcgga aaaaggcaat atcaagggta tgcagtgcgt ccggatgaaa     1560

cttggcgagc cggacgattc tggccggcgc cggcccgtac ccgtgccggg aacggagttt     1620

ttcctgccct gcgatattgt ggtgccggct atcggccagg cggccgacct gtctttcctg     1680

gacggccgga tcgaggtggg caaacgtggt accatcagtg ttgacccggt gaccctggct     1740

accagtgtcc ccggcgtctt cgccggcggc gatatcgtcc tgggggccag gacggtagtt     1800

gaggccgtgg cccagggcaa ccgggcggca gtttccattg accagtacct gcgccagggg     1860

accaccagtc ccacggtaga ggacgagctg gacgcgtggc tggaaaaggt cggcgtctat     1920

gacccggaag aggatgtagg aatctacggc ggccggccgc ggcaggcaga aagggtggcg     1980

ccgttggccg agcgggtaaa ggacttccgg gaagttgaag gcggcttcga tttctacgca     2040

ggcagggccg aagccgaacg ctgcctgcgc tgttaccggg tcgggatgat ggtcctggcc     2100

ggagaggggg agagtaatgg ttaa                                            2124


<210>  31
<211>  707
<212>  PRT
<213>  Moorella thermoaceticum formate dehydrogenase (NAD+) beta subunit

<400>  31

Met Gly Glu Val Val Phe Ser Thr Trp Gly Gly Lys Val Val Asp His 
1               5                   10                  15      


Arg Gly Gly Pro Ser Gly Gly Gly Pro Ser Trp Ala Gly Glu Phe Gly 
            20                  25                  30          


Gly Arg Gln Leu Lys Ala Phe Ile Gly Trp Asp Gly Leu Val Val Thr 
        35                  40                  45              


Asp Pro Ala Val Asp Leu Leu Ala Ala Leu Gln Ala Tyr Tyr Gln Ala 
    50                  55                  60                  


Val Gln Gly Glu Ser Cys Gly Arg Cys Val Pro Cys Arg Val Gly Thr 
65                  70                  75                  80  


Arg Val Ile Tyr Asn Val Leu Val Arg Ile Ala Gly Gly Glu Gly Leu 
                85                  90                  95      


Pro Ser Asp Leu Asp Leu Leu Arg Arg Val Ala Trp Ile Val Arg Asp 
            100                 105                 110         


Gly Ser Leu Cys Glu Leu Gly Gln Ala Gly Ala Lys Ala Val Leu Asp 
        115                 120                 125             


Phe Leu Asp Tyr Tyr Ser Glu Ala Leu Arg Pro Phe Leu Glu Asp Ser 
    130                 135                 140                 


Gly Arg Val Ala Gly Gly Gln Arg Arg Pro Gly Pro Gly Gly Arg Val 
145                 150                 155                 160 


Gln Val Leu Ala Ser Gly Arg Val Leu Val Gly Asn Asp Arg Gly Lys 
                165                 170                 175     


Gly Ala Ala Ala Ala Ser Pro Ala Ala Gly Leu Thr Tyr Lys Pro Phe 
            180                 185                 190         


Val Thr Ala Pro Cys Leu Lys Arg Cys Pro Ala His Leu Asp Ile Pro 
        195                 200                 205             


Ala Tyr Ile Asp Ala Ile Lys Asp Gly Arg Tyr Glu Glu Ser Leu Ala 
    210                 215                 220                 


Ile Ile Arg Gln Arg Thr Ala Leu Ala Gly Val Leu Gly Arg Val Cys 
225                 230                 235                 240 


Val His Pro Cys Glu Glu Asn Cys Arg Arg Gly Asn Val Asp Glu Pro 
                245                 250                 255     


Leu Ala Ile Arg Gly Leu Lys Arg Phe Val Ala Asp Tyr Glu Val Lys 
            260                 265                 270         


Arg Gly Arg Arg Pro Val Ala Val Cys Gly Gly Asn Leu Phe Thr Gly 
        275                 280                 285             


Pro Trp Arg Pro Ala Gly Gln Ala Gly Gly Glu Glu Thr Thr Ala Val 
    290                 295                 300                 


Thr Ser Gly Lys Lys Val Ala Ile Ile Gly Ala Gly Pro Ala Gly Leu 
305                 310                 315                 320 


Ser Ala Ala Tyr Gln Leu Ala Gly Arg Gly Tyr Lys Val Thr Ile Phe 
                325                 330                 335     


Glu Ala Leu Pro Val Ala Gly Gly Met Leu Ala Val Gly Ile Pro Ser 
            340                 345                 350         


Tyr Arg Leu Pro Arg Asp Ile Leu Ala Gly Glu Ile Glu Ala Ile Lys 
        355                 360                 365             


Ala Leu Gly Val Thr Ile Asn Leu Asn Thr Arg Val Gly Val Asp Val 
    370                 375                 380                 


Thr Met Asp Gln Leu Gln Arg Asp Tyr Asp Ala Val Phe Ile Ala Thr 
385                 390                 395                 400 


Gly Leu His Ala Ser Ser Arg Met Gly Val Ala Gly Glu Asp Glu Gly 
                405                 410                 415     


Tyr Gly Gly Phe Ile Pro Gly Val Lys Phe Leu Arg Asp Leu Asn Leu 
            420                 425                 430         


Asp Arg Cys Pro Ser Leu Glu Gly Lys Val Val Ala Val Val Gly Gly 
        435                 440                 445             


Gly Asn Val Ala Met Asp Cys Ala Arg Ser Ala Leu Arg Arg Gly Ala 
    450                 455                 460                 


Arg Glu Val His Leu Ile Tyr Arg Arg Ser Arg Ala Glu Met Pro Ala 
465                 470                 475                 480 


His Ala Thr Glu Val Arg Asp Ala Glu Ala Glu Gly Val Ile Tyr His 
                485                 490                 495     


Phe Leu Val Asn Pro Thr Ala Leu Val Ala Glu Lys Gly Asn Ile Lys 
            500                 505                 510         


Gly Met Gln Cys Val Arg Met Lys Leu Gly Glu Pro Asp Asp Ser Gly 
        515                 520                 525             


Arg Arg Arg Pro Val Pro Val Pro Gly Thr Glu Phe Phe Leu Pro Cys 
    530                 535                 540                 


Asp Ile Val Val Pro Ala Ile Gly Gln Ala Ala Asp Leu Ser Phe Leu 
545                 550                 555                 560 


Asp Gly Arg Ile Glu Val Gly Lys Arg Gly Thr Ile Ser Val Asp Pro 
                565                 570                 575     


Val Thr Leu Ala Thr Ser Val Pro Gly Val Phe Ala Gly Gly Asp Ile 
            580                 585                 590         


Val Leu Gly Ala Arg Thr Val Val Glu Ala Val Ala Gln Gly Asn Arg 
        595                 600                 605             


Ala Ala Val Ser Ile Asp Gln Tyr Leu Arg Gln Gly Thr Thr Ser Pro 
    610                 615                 620                 


Thr Val Glu Asp Glu Leu Asp Ala Trp Leu Glu Lys Val Gly Val Tyr 
625                 630                 635                 640 


Asp Pro Glu Glu Asp Val Gly Ile Tyr Gly Gly Arg Pro Arg Gln Ala 
                645                 650                 655     


Glu Arg Val Ala Pro Leu Ala Glu Arg Val Lys Asp Phe Arg Glu Val 
            660                 665                 670         


Glu Gly Gly Phe Asp Phe Tyr Ala Gly Arg Ala Glu Ala Glu Arg Cys 
        675                 680                 685             


Leu Arg Cys Tyr Arg Val Gly Met Met Val Leu Ala Gly Glu Gly Glu 
    690                 695                 700                 


Ser Asn Gly 
705         


<210>  32
<211>  1173
<212>  DNA
<213>  C.thermocellum malic enzyme

<400>  32
atggattaca gaaaagaatc actaaggctt cacggtgagt ggaagggtaa aattgaggtt       60

atacacaagg tacctgtttc aaccaaggaa gagttgtcgc ttgcttatac accgggtgtt      120

gcagaaccat gtcttgcaat tcagaaagat gttaatcttt cttatgaata tacaagacgt      180

tggaacctgg tagcggttat taccgacggt acggcggttt tagggctcgg agacatagga      240

cctgaagccg gaatgcctgt tatggaaggt aaatgcgtac tcttcaagag gtttggtgat      300

gtggacgcat ttccgctctg tatcaaatca aaagacgtag atgaaattgt aaagacaatc      360

aagctcatct ccggaagctt tggcggtata aacctcgaag atatatccgc tccgagatgc      420

tttgaaatag aaagaagact caaagaggaa tgtgacattc caatattcca tgatgaccag      480

cacggtacag ccgttgttac tgttgcagca atgatcaatg cattaaagct tgtcaacaag      540

aaaatcgagg atatagaagt tgttgtaaac ggttcaggtg ctgccggcat agctgtaaca      600

agactgctca tgagtatggg gcttaagaaa gttatccttt gcgataccaa aggtgcaatt      660

tatgatggaa gagacaactt aaacagtgaa aaagccctga ttgctaaaat ctcgaacctc      720

gagaaaaaga aaggtactct tgaagatgta atcaagggag ctgacgtatt catcggtctt      780

tccgttccag gaacagttac aaaggatatg gtaaaatcca tggcaaagga tccgattatc      840

tttgctatgg caaatcctac tcctgaaata atgcctgatg aagcaaaaga agcaggagca      900

aaggtagtgg gtaccggaag atccgacttc ccgaaccaga taaacaacgt tcttgcgttc      960

cccggaatat tcagaggtgc gcttgatgta agagcaagag atatcaatga tgaaatgaag     1020

atagccgctg caaaagcaat agcttctctg gtaagcgatg aagagctcaa tcctgacttc     1080

attcttccgc tcccatttga cccaagagtc ggaaaaacag ttgctgcagc agttgctgaa     1140

gcagcaagaa aaaccggagt tgcaagaata taa                                  1173


<210>  33
<211>  390
<212>  PRT
<213>  C.thermocellum malic enzyme

<400>  33

Met Asp Tyr Arg Lys Glu Ser Leu Arg Leu His Gly Glu Trp Lys Gly 
1               5                   10                  15      


Lys Ile Glu Val Ile His Lys Val Pro Val Ser Thr Lys Glu Glu Leu 
            20                  25                  30          


Ser Leu Ala Tyr Thr Pro Gly Val Ala Glu Pro Cys Leu Ala Ile Gln 
        35                  40                  45              


Lys Asp Val Asn Leu Ser Tyr Glu Tyr Thr Arg Arg Trp Asn Leu Val 
    50                  55                  60                  


Ala Val Ile Thr Asp Gly Thr Ala Val Leu Gly Leu Gly Asp Ile Gly 
65                  70                  75                  80  


Pro Glu Ala Gly Met Pro Val Met Glu Gly Lys Cys Val Leu Phe Lys 
                85                  90                  95      


Arg Phe Gly Asp Val Asp Ala Phe Pro Leu Cys Ile Lys Ser Lys Asp 
            100                 105                 110         


Val Asp Glu Ile Val Lys Thr Ile Lys Leu Ile Ser Gly Ser Phe Gly 
        115                 120                 125             


Gly Ile Asn Leu Glu Asp Ile Ser Ala Pro Arg Cys Phe Glu Ile Glu 
    130                 135                 140                 


Arg Arg Leu Lys Glu Glu Cys Asp Ile Pro Ile Phe His Asp Asp Gln 
145                 150                 155                 160 


His Gly Thr Ala Val Val Thr Val Ala Ala Met Ile Asn Ala Leu Lys 
                165                 170                 175     


Leu Val Asn Lys Lys Ile Glu Asp Ile Glu Val Val Val Asn Gly Ser 
            180                 185                 190         


Gly Ala Ala Gly Ile Ala Val Thr Arg Leu Leu Met Ser Met Gly Leu 
        195                 200                 205             


Lys Lys Val Ile Leu Cys Asp Thr Lys Gly Ala Ile Tyr Asp Gly Arg 
    210                 215                 220                 


Asp Asn Leu Asn Ser Glu Lys Ala Leu Ile Ala Lys Ile Ser Asn Leu 
225                 230                 235                 240 


Glu Lys Lys Lys Gly Thr Leu Glu Asp Val Ile Lys Gly Ala Asp Val 
                245                 250                 255     


Phe Ile Gly Leu Ser Val Pro Gly Thr Val Thr Lys Asp Met Val Lys 
            260                 265                 270         


Ser Met Ala Lys Asp Pro Ile Ile Phe Ala Met Ala Asn Pro Thr Pro 
        275                 280                 285             


Glu Ile Met Pro Asp Glu Ala Lys Glu Ala Gly Ala Lys Val Val Gly 
    290                 295                 300                 


Thr Gly Arg Ser Asp Phe Pro Asn Gln Ile Asn Asn Val Leu Ala Phe 
305                 310                 315                 320 


Pro Gly Ile Phe Arg Gly Ala Leu Asp Val Arg Ala Arg Asp Ile Asn 
                325                 330                 335     


Asp Glu Met Lys Ile Ala Ala Ala Lys Ala Ile Ala Ser Leu Val Ser 
            340                 345                 350         


Asp Glu Glu Leu Asn Pro Asp Phe Ile Leu Pro Leu Pro Phe Asp Pro 
        355                 360                 365             


Arg Val Gly Lys Thr Val Ala Ala Ala Val Ala Glu Ala Ala Arg Lys 
    370                 375                 380                 


Thr Gly Val Ala Arg Ile 
385                 390 


<210>  34
<211>  1622
<212>  DNA
<213>  Geobacillus stearothermophilus malic acid

<400>  34
ctgcagaaag gggcatgtac aataagggtt gtctttttcc ggttcatagg ataacatttg       60

taccgtaaga tgatttactg atttaaacaa aaatagagaa aaggaaggac gattggttat      120

ggcattacca ggcggggcag ccatgaacat tacgattcgt ctccagttcg aaaaagatat      180

cgtctcattc agcgatatcg ccgccgcgat tggcaaagcg ggcggggaca ttgtcgggat      240

tgacgtcatt tcgtcaagca aagttcacac ggtgcgcgat attaccgtca gcgccctcga      300

tacgaagcag tgcgacttga tcattgaggc gctgaaaaaa attcgcggcg tcaaaattgt      360

gaacgtttcc gaccgcacgt ttttgatgca catcgggggg aaaattgaaa caaattcaaa      420

aatcccagtg aaaacacgcg acgacttgtc gcgggtatac acgccgggcg tggcgcgcgt      480

ctgcacggcg attgccgaag atccgcgaaa ggcgtattcg ctgacgatta aacggaatac      540

ggtcgccgtt gtctcggacg gcacggcggt gcttgggctt ggcgacatcg gtccgtacgc      600

ggcgatgcca gtcatggaag ggaaagcgat gctgtttaag gaatttgccg gagtggacgc      660

gttcccgatt tgtttggata cgaaagacac ggaagaaatc attcaaattg tgaaagcgat      720

cgccccggcg tttggcggca ttaacctgga ggacatttcc gccccacgtt gttttgaaat      780

tgaaaagcgg ctgaaagaag agctggatat tcccgtgttt catgatgacc agcatggcac      840

ggcggtcgtg ctcttggcgg gactgcttaa cgcgctgaaa atcgttgaca aaaagctcga      900

agacattaaa gtcgtcttaa ccggcatcgg tgcggccggc atcgcctgca cgaaaatttt      960

gcttgcggcc ggcgtgcgca acattatcgg ggtcgaccgc catggcgcca tccaccgcga     1020

tgaaacgtac gaaaatccgt actggcaaga gtatgcgcaa ctcacgaacc cggataatct     1080

gaaaggaagc ttgtccgatg tcatcgccgg cgcagatgta tttatcggcg tttcggctcc     1140

gggcatttta aaagtggaag atgtgaaaaa aatggcgcgc gatccgatcg tgtttgcgat     1200

ggccaacccg attccggaaa ttgatccgga gctggccgaa ccgtacgtgc gcgtcatggc     1260

gaccgggcgt tccgactatc cgaaccaaat caacaacgtc ctttgcttcc cgggcatttt     1320

ccgcggggcg cttgactgcc gggcgagaga aattaacgag gaaatgaagc tcgctgccgc     1380

gaaggcgatt gcctctgtcg tgacggagga tgaattgaac gaaacataca tcatcccgag     1440

cgtcttcaac agcaaagtcg tcgaacgcgt cagacaagcg gtcgtcgaag cggcttaccg     1500

cactggggtg gcgcggaagg acaatatccc ggttggcgga tatacagggc agtaaagcaa     1560

ggaatgcgat ggaaccgacg gacggcttcc tttatagggg aagccgtttt ttttggctgc     1620

ag                                                                    1622


<210>  35
<211>  1011
<212>  DNA
<213>  C. thermocellum Glyceraldehyde-3-phosphate dehydrogenase

<400>  35
atggcagtaa aaattggtat caacggtttt ggacgtatcg gtcgtcttgt gttcagggcc       60

agtctcaaca acccgaacgt tgaggttgta ggtataaacg acccatttat tgaccttgaa      120

tacatgcaat atatgttaaa gtatgataca gtacatggtc agttcaaagg tgaaatttca      180

caagacaacg gaaaactcgt tgtaaacggc agaaaaataa gtgtttatgg tttcactgat      240

cctgctgaga ttccgtggag cgaatgcggt gcggaataca tcgttgaatc aacgggtgta      300

ttcacaacta ctgaaaaggc ttcagctcac ttcaagggcg gtgcaaagaa ggtagttatc      360

agtgctcctt cggcagatgc tccgatgttt gttatgggtg taaaccatga taaatacaca      420

aaggatatga acgttgtatc taacgcttca tgtacaacaa actgccttgc tcctctggct      480

aaagttatac atgaaaactt cggaatcgta gaaggcttga tgactactgt acacgctaca      540

actgcaactc aaaagactgt tgacggtcct tcaaagaaag actggagagg cggacgtgca      600

gcagcaggca acatcattcc ttcatcaaca ggagctgcaa aggcagttgg aaaggttatc      660

cctgaattga acggtaagtt gacaggtatg gctttcagag ttccgactct tgacgtatct      720

gttgttgact tgacttgccg tcttgaaaaa ccggctactt atgatgaaat caaagccgct      780

gttaagaaag cttcagaaaa tgaacttaag ggtattttgg gatacactga ggatgcggtt      840

gtttcttcag acttcatcgg cgatccccgc acttcaattt tcgatgctga agcaggtatt      900

tctctcaaca gcaactttgt aaaacttgtt gcctggtacg acaatgaatg gggttattca      960

aacaaagttg ttgatttgat agttcatatg gcttcggttg atgcaaaata a              1011


<210>  36
<211>  336
<212>  PRT
<213>  C. thermocellum Glyceraldehyde-3-phosphate dehydrogenase

<400>  36

Met Ala Val Lys Ile Gly Ile Asn Gly Phe Gly Arg Ile Gly Arg Leu 
1               5                   10                  15      


Val Phe Arg Ala Ser Leu Asn Asn Pro Asn Val Glu Val Val Gly Ile 
            20                  25                  30          


Asn Asp Pro Phe Ile Asp Leu Glu Tyr Met Gln Tyr Met Leu Lys Tyr 
        35                  40                  45              


Asp Thr Val His Gly Gln Phe Lys Gly Glu Ile Ser Gln Asp Asn Gly 
    50                  55                  60                  


Lys Leu Val Val Asn Gly Arg Lys Ile Ser Val Tyr Gly Phe Thr Asp 
65                  70                  75                  80  


Pro Ala Glu Ile Pro Trp Ser Glu Cys Gly Ala Glu Tyr Ile Val Glu 
                85                  90                  95      


Ser Thr Gly Val Phe Thr Thr Thr Glu Lys Ala Ser Ala His Phe Lys 
            100                 105                 110         


Gly Gly Ala Lys Lys Val Val Ile Ser Ala Pro Ser Ala Asp Ala Pro 
        115                 120                 125             


Met Phe Val Met Gly Val Asn His Asp Lys Tyr Thr Lys Asp Met Asn 
    130                 135                 140                 


Val Val Ser Asn Ala Ser Cys Thr Thr Asn Cys Leu Ala Pro Leu Ala 
145                 150                 155                 160 


Lys Val Ile His Glu Asn Phe Gly Ile Val Glu Gly Leu Met Thr Thr 
                165                 170                 175     


Val His Ala Thr Thr Ala Thr Gln Lys Thr Val Asp Gly Pro Ser Lys 
            180                 185                 190         


Lys Asp Trp Arg Gly Gly Arg Ala Ala Ala Gly Asn Ile Ile Pro Ser 
        195                 200                 205             


Ser Thr Gly Ala Ala Lys Ala Val Gly Lys Val Ile Pro Glu Leu Asn 
    210                 215                 220                 


Gly Lys Leu Thr Gly Met Ala Phe Arg Val Pro Thr Leu Asp Val Ser 
225                 230                 235                 240 


Val Val Asp Leu Thr Cys Arg Leu Glu Lys Pro Ala Thr Tyr Asp Glu 
                245                 250                 255     


Ile Lys Ala Ala Val Lys Lys Ala Ser Glu Asn Glu Leu Lys Gly Ile 
            260                 265                 270         


Leu Gly Tyr Thr Glu Asp Ala Val Val Ser Ser Asp Phe Ile Gly Asp 
        275                 280                 285             


Pro Arg Thr Ser Ile Phe Asp Ala Glu Ala Gly Ile Ser Leu Asn Ser 
    290                 295                 300                 


Asn Phe Val Lys Leu Val Ala Trp Tyr Asp Asn Glu Trp Gly Tyr Ser 
305                 310                 315                 320 


Asn Lys Val Val Asp Leu Ile Val His Met Ala Ser Val Asp Ala Lys 
                325                 330                 335     


<210>  37
<211>  2229
<212>  DNA
<213>  C.thermocellum Pyruvate formate lyase

<400>  37
atggatgcat ggcgcggatt taataaaggc aactggtgcc aggaaattga cgttcgtgat       60

tttataatta gaaattatac tccttatgaa ggcgatgaaa gctttcttgt aggacctacg      120

gatagaacgc ggaaactttg ggagaaggtt tccgaactgt taaagaaaga acgggagaac      180

ggcggggtat tggatgttga tacccataca atttcaacga ttacgtctca taaacctgga      240

tatatagata aagaacttga agttattgtc gggcttcaga cggatgagcc tttaaaaaga      300

gccataatgc cgtttggcgg tatacgtatg gtgattaagg gagccgaagc ttatggccac      360

agtgtggacc ctcaggttgt tgaaatattc acaaagtaca gaaagactca taaccaggga      420

gtttatgatg tatatactcc cgaaatgaga aaagccaaaa aagccgggat tattacagga      480

cttcccgacg catacggcag aggaagaata attggcgatt acagaagggt tgcactttat      540

ggcgttgaca ggctgattgc tgaaaaagag aaagaaatgg caagtcttga aagagattac      600

attgactatg agactgttcg agacagagaa gaaataagcg agcagattaa atctttaaaa      660

caacttaaag aaatggcttt aagttacggt tttgacatat cttgtcctgc aaaggatgcc      720

agagaagcct ttcaatggtt gtattttgca tatcttgcag cagtcaagga acagaacggc      780

gcggcaatga gtattggaag aatttcgact ttccttgaca tatacattga aagggatctc      840

aaagaaggaa aactcacgga ggagttggct caggaactgg ttgaccagct ggttataaag      900

ctgagaattg tgagattttt gagaactcct gagtatgaaa agctcttcag cggagacccc      960

acttgggtaa ccgaaagtat cggaggtatg gcgctggatg gaagaacgct ggttacaaaa     1020

tcttcgttca ggtttttgca cactcttttc aacctgggac atgcaccgga gcccaacctt     1080

acagtacttt ggtccgtcaa tcttcccgaa ggctttaaaa agtactgtgc aaaggtatca     1140

attcattcaa gctccatcca gtatgaaagc gacgacataa tgaggaaaca ctggggagac     1200

gattatggaa tagcatgctg tgtttctgct atgagaattg gaaaacagat gcagttcttc     1260

ggtgcaagat gcaatcttgc aaaagctctt ctttacgcta ttaacggcgg aaaggatgaa     1320

atgacgggag aacagattgc tccgatgttt gcaccggtgg aaaccgaata ccttgattac     1380

gaggacgtaa tgaagaggtt tgacatggtg cttgactggg tggcaaggct ttatatgaac     1440

accctcaata taattcacta catgcatgac aaatatgcct atgaggcgct gcagatggca     1500

ttgcatgaca aagacgtgtt caggacgatg gcatgcggaa tagccggttt gtctgtggtg     1560

gcagactccc ttagcgcgat aaaatatgca aaggttaaac cgatacgcaa tgaaaacaac     1620

ctcgttgttg actacgaagt tgagggtgat tatcctaaat tcggaaataa cgacgaacgt     1680

gttgatgaaa ttgcagtgca agtagtaaaa atgttcatga acaagcttag aaagcaaagg     1740

gcttacagaa gtgccactcc gaccctttcc atacttacca taacttcaaa cgtggtatat     1800

ggaaagaaaa ccggaaacac tcctgacggc agaaaagctg gagaaccttt ggcgccggga     1860

gcaaatccga tgcatggaag ggatataaac ggagcattgg ctgtactgaa cagtattgcg     1920

aagcttccct atgaatatgc ccaggacggc atttcatata ctttctccat aattccaaaa     1980

gctctgggaa gagacgagga aaccagaata aacaatctta aatcaatgct tgacggatat     2040

ttcaagcagg gcggccacca cataaatgta aatgtgtttg aaaaagagac actgttagat     2100

gccatggaac atccggaaaa atatccacaa cttaccataa gagtgtccgg gtatgcagtg     2160

aactttataa agcttacacg ggagcaacag ctggatgtta ttaacagaac gattcacgga     2220

aagatttaa                                                             2229


<210>  38
<211>  742
<212>  PRT
<213>  C.thermocellum Pyruvate formate lyase

<400>  38

Met Asp Ala Trp Arg Gly Phe Asn Lys Gly Asn Trp Cys Gln Glu Ile 
1               5                   10                  15      


Asp Val Arg Asp Phe Ile Ile Arg Asn Tyr Thr Pro Tyr Glu Gly Asp 
            20                  25                  30          


Glu Ser Phe Leu Val Gly Pro Thr Asp Arg Thr Arg Lys Leu Trp Glu 
        35                  40                  45              


Lys Val Ser Glu Leu Leu Lys Lys Glu Arg Glu Asn Gly Gly Val Leu 
    50                  55                  60                  


Asp Val Asp Thr His Thr Ile Ser Thr Ile Thr Ser His Lys Pro Gly 
65                  70                  75                  80  


Tyr Ile Asp Lys Glu Leu Glu Val Ile Val Gly Leu Gln Thr Asp Glu 
                85                  90                  95      


Pro Leu Lys Arg Ala Ile Met Pro Phe Gly Gly Ile Arg Met Val Ile 
            100                 105                 110         


Lys Gly Ala Glu Ala Tyr Gly His Ser Val Asp Pro Gln Val Val Glu 
        115                 120                 125             


Ile Phe Thr Lys Tyr Arg Lys Thr His Asn Gln Gly Val Tyr Asp Val 
    130                 135                 140                 


Tyr Thr Pro Glu Met Arg Lys Ala Lys Lys Ala Gly Ile Ile Thr Gly 
145                 150                 155                 160 


Leu Pro Asp Ala Tyr Gly Arg Gly Arg Ile Ile Gly Asp Tyr Arg Arg 
                165                 170                 175     


Val Ala Leu Tyr Gly Val Asp Arg Leu Ile Ala Glu Lys Glu Lys Glu 
            180                 185                 190         


Met Ala Ser Leu Glu Arg Asp Tyr Ile Asp Tyr Glu Thr Val Arg Asp 
        195                 200                 205             


Arg Glu Glu Ile Ser Glu Gln Ile Lys Ser Leu Lys Gln Leu Lys Glu 
    210                 215                 220                 


Met Ala Leu Ser Tyr Gly Phe Asp Ile Ser Cys Pro Ala Lys Asp Ala 
225                 230                 235                 240 


Arg Glu Ala Phe Gln Trp Leu Tyr Phe Ala Tyr Leu Ala Ala Val Lys 
                245                 250                 255     


Glu Gln Asn Gly Ala Ala Met Ser Ile Gly Arg Ile Ser Thr Phe Leu 
            260                 265                 270         


Asp Ile Tyr Ile Glu Arg Asp Leu Lys Glu Gly Lys Leu Thr Glu Glu 
        275                 280                 285             


Leu Ala Gln Glu Leu Val Asp Gln Leu Val Ile Lys Leu Arg Ile Val 
    290                 295                 300                 


Arg Phe Leu Arg Thr Pro Glu Tyr Glu Lys Leu Phe Ser Gly Asp Pro 
305                 310                 315                 320 


Thr Trp Val Thr Glu Ser Ile Gly Gly Met Ala Leu Asp Gly Arg Thr 
                325                 330                 335     


Leu Val Thr Lys Ser Ser Phe Arg Phe Leu His Thr Leu Phe Asn Leu 
            340                 345                 350         


Gly His Ala Pro Glu Pro Asn Leu Thr Val Leu Trp Ser Val Asn Leu 
        355                 360                 365             


Pro Glu Gly Phe Lys Lys Tyr Cys Ala Lys Val Ser Ile His Ser Ser 
    370                 375                 380                 


Ser Ile Gln Tyr Glu Ser Asp Asp Ile Met Arg Lys His Trp Gly Asp 
385                 390                 395                 400 


Asp Tyr Gly Ile Ala Cys Cys Val Ser Ala Met Arg Ile Gly Lys Gln 
                405                 410                 415     


Met Gln Phe Phe Gly Ala Arg Cys Asn Leu Ala Lys Ala Leu Leu Tyr 
            420                 425                 430         


Ala Ile Asn Gly Gly Lys Asp Glu Met Thr Gly Glu Gln Ile Ala Pro 
        435                 440                 445             


Met Phe Ala Pro Val Glu Thr Glu Tyr Leu Asp Tyr Glu Asp Val Met 
    450                 455                 460                 


Lys Arg Phe Asp Met Val Leu Asp Trp Val Ala Arg Leu Tyr Met Asn 
465                 470                 475                 480 


Thr Leu Asn Ile Ile His Tyr Met His Asp Lys Tyr Ala Tyr Glu Ala 
                485                 490                 495     


Leu Gln Met Ala Leu His Asp Lys Asp Val Phe Arg Thr Met Ala Cys 
            500                 505                 510         


Gly Ile Ala Gly Leu Ser Val Val Ala Asp Ser Leu Ser Ala Ile Lys 
        515                 520                 525             


Tyr Ala Lys Val Lys Pro Ile Arg Asn Glu Asn Asn Leu Val Val Asp 
    530                 535                 540                 


Tyr Glu Val Glu Gly Asp Tyr Pro Lys Phe Gly Asn Asn Asp Glu Arg 
545                 550                 555                 560 


Val Asp Glu Ile Ala Val Gln Val Val Lys Met Phe Met Asn Lys Leu 
                565                 570                 575     


Arg Lys Gln Arg Ala Tyr Arg Ser Ala Thr Pro Thr Leu Ser Ile Leu 
            580                 585                 590         


Thr Ile Thr Ser Asn Val Val Tyr Gly Lys Lys Thr Gly Asn Thr Pro 
        595                 600                 605             


Asp Gly Arg Lys Ala Gly Glu Pro Leu Ala Pro Gly Ala Asn Pro Met 
    610                 615                 620                 


His Gly Arg Asp Ile Asn Gly Ala Leu Ala Val Leu Asn Ser Ile Ala 
625                 630                 635                 640 


Lys Leu Pro Tyr Glu Tyr Ala Gln Asp Gly Ile Ser Tyr Thr Phe Ser 
                645                 650                 655     


Ile Ile Pro Lys Ala Leu Gly Arg Asp Glu Glu Thr Arg Ile Asn Asn 
            660                 665                 670         


Leu Lys Ser Met Leu Asp Gly Tyr Phe Lys Gln Gly Gly His His Ile 
        675                 680                 685             


Asn Val Asn Val Phe Glu Lys Glu Thr Leu Leu Asp Ala Met Glu His 
    690                 695                 700                 


Pro Glu Lys Tyr Pro Gln Leu Thr Ile Arg Val Ser Gly Tyr Ala Val 
705                 710                 715                 720 


Asn Phe Ile Lys Leu Thr Arg Glu Gln Gln Leu Asp Val Ile Asn Arg 
                725                 730                 735     


Thr Ile His Gly Lys Ile 
            740         


<210>  39
<211>  717
<212>  DNA
<213>  C.thermocellum pyruvate formate lyase activating enzyme

<400>  39
atgacattaa agggcaggat acactcattt gaatcttttg ggacactgga cggaccgggt       60

ataagatttg tggttttcat gcagggctgt cccttgcgtt gtatatattg ccacaacagg      120

gatacctggg atgttaatgc ggggagtgag tacactcccc ggcaagtaat tgatgaaatg      180

atgaaataca tagactatat aaaggtctcc ggaggcggaa taactgttac cggcggggag      240

cctgttctcc aggccgattt tgtggccgag gtgttcagac ttgcaaaaga gcagggagtg      300

catacggcgc tggataccaa tggatttgct gacatagaga aggttgaaag gcttataaaa      360

tacaccgatc ttgtattgct ggatataaag catgcccggg aggataaaca taagataatt      420

accggtgtgt ccaacgaaaa aatcaagcgt tttgcgctgt atctttcgga ccagggagtg      480

cctatctgga taagatatgt ccttgtcccc ggatataccg acgatgaaga tgaccttaaa      540

atggcggctg atttcataaa aaagcttaaa acggtggaaa aaatcgaagt tcttccttat      600

cacaacatgg gagcatacaa atgggaaaaa cttggtcaga aatacatgct tgaaggagta      660

aaggggccga gtgcgcaaga ggtggaaaaa gcaaagagga ttctgtcagg caaataa         717


<210>  40
<211>  238
<212>  PRT
<213>  C.thermocellum pyruvate formate lyase activating enzyme

<400>  40

Met Thr Leu Lys Gly Arg Ile His Ser Phe Glu Ser Phe Gly Thr Leu 
1               5                   10                  15      


Asp Gly Pro Gly Ile Arg Phe Val Val Phe Met Gln Gly Cys Pro Leu 
            20                  25                  30          


Arg Cys Ile Tyr Cys His Asn Arg Asp Thr Trp Asp Val Asn Ala Gly 
        35                  40                  45              


Ser Glu Tyr Thr Pro Arg Gln Val Ile Asp Glu Met Met Lys Tyr Ile 
    50                  55                  60                  


Asp Tyr Ile Lys Val Ser Gly Gly Gly Ile Thr Val Thr Gly Gly Glu 
65                  70                  75                  80  


Pro Val Leu Gln Ala Asp Phe Val Ala Glu Val Phe Arg Leu Ala Lys 
                85                  90                  95      


Glu Gln Gly Val His Thr Ala Leu Asp Thr Asn Gly Phe Ala Asp Ile 
            100                 105                 110         


Glu Lys Val Glu Arg Leu Ile Lys Tyr Thr Asp Leu Val Leu Leu Asp 
        115                 120                 125             


Ile Lys His Ala Arg Glu Asp Lys His Lys Ile Ile Thr Gly Val Ser 
    130                 135                 140                 


Asn Glu Lys Ile Lys Arg Phe Ala Leu Tyr Leu Ser Asp Gln Gly Val 
145                 150                 155                 160 


Pro Ile Trp Ile Arg Tyr Val Leu Val Pro Gly Tyr Thr Asp Asp Glu 
                165                 170                 175     


Asp Asp Leu Lys Met Ala Ala Asp Phe Ile Lys Lys Leu Lys Thr Val 
            180                 185                 190         


Glu Lys Ile Glu Val Leu Pro Tyr His Asn Met Gly Ala Tyr Lys Trp 
        195                 200                 205             


Glu Lys Leu Gly Gln Lys Tyr Met Leu Glu Gly Val Lys Gly Pro Ser 
    210                 215                 220                 


Ala Gln Glu Val Glu Lys Ala Lys Arg Ile Leu Ser Gly Lys 
225                 230                 235             


<210>  41
<211>  2652
<212>  DNA
<213>  C.thermocellum pyruvate-phosphate dikinase

<400>  41
atggcaaagt atgtgtattt gtttagtgaa ggcaatgcat caatgagaga cctgcttgga       60

ggaaaaggtg ccaatcttgc agaaatgaca agtttgggac ttccggtacc cagaggtttt      120

accattacca cggaagcttg tacacgctac tatcaagatg gaaaagtcat tgccaaggaa      180

atagaagatg aaatatacag aactatggag aagcttgaag agattgtcgg gaagaaattc      240

ggtgacccat caaatccgtt tcttgtttcc gttcgttccg gtgccagagt atcaatgccc      300

ggtatgatgg ataccatatt aaatctcgga cttaatgatg aagttgttgt aggtcttgca      360

aagcttacca ataatgaaag attcgcatat gacagctata gaagatttat tcaaatgttc      420

agcgatgttg tcatggaggt ggaaaagtcc aagtttgaag ctattcttga tgctgtgaaa      480

gaagaaaaca actgtgaaaa tgactgcgac ctttctgccg aaaatttaaa ggaagtagtc      540

agaagataca aggagctgtt caagaaagaa aagggatttg attttccgca ggatccgaaa      600

acacagttga tggaagccgt aaaagccgtt ttccgttcat gggaaaatcc aagggctatt      660

gtatatagaa gattaaatga catccccggc gactggggta ctgcagttaa cgttcaggaa      720

atggtttatg gaaatatggg aaatgattcg ggtacgggag ttgcctttac aaggaacccg      780

gctacgggag aaaagaagct ttatggtgaa ttccttatga atgcccaggg agaagacgtt      840

gttgcaggta tcagaactcc ccagtcaatt gaccagctga aagaagtaat gcctgatgta      900

tacaatcagt ttgtggagat agccgaaaaa cttgaaagac attatagaga tatgcaggat      960

atggagttta caattgaaag aggaaaactc ttcatgctcc agacaagaaa cggtaaaagg     1020

actgctgcgg ctgctttaaa aatagctgtt gatttggtaa atgagggaat ggtcacaaaa     1080

gaagaagcaa ttttaaaagt cgacccgaaa cagcttgata cactgctcca tccaaatttc     1140

gaaccttcag cgctgaaaaa tgcaaaacct atagcaaagg gattgccggc ttcaccggga     1200

gctgctaccg gaaagattta ctttagagcc gaggatgcgg tggaagcggc caaaaacgga     1260

gaaaaagaca tcattcttgt aagacttgaa acttcacccg aagatattga gggtatgcat     1320

gtatccaaag gaatacttac aggccgtggt ggaatgacat ctcatgctgc agttgttgca     1380

cgcggtatgg gtacttgctg cgttgccggc tgcagtgaaa taagaataaa tgaggaagag     1440

aaatactttg tagataaaaa cggaaagaaa tatgttgagg gtgattggat ttcccttgac     1500

ggttccacag gtaatgttta tggggaaaag cttcctacag tggagcctga aatgaccggc     1560

gactttgcca cacttatgca gtgggccgat gaaatcagaa ctcttaagat tagaaccaat     1620

gccgatactc cggctgatgc catccaggca agaaagttcg gtgcggaagg tatcggactt     1680

tgccgtacgg agcatatgtt cttcgattct gacagaattc cggcaatgag agaaatgata     1740

gttgcaagaa ccgaagaaca gagaagaaag gctttggata aactcctgcc gatgcagaga     1800

aaagattttg aagaactgtt tactgcaatg gaaggctatc ctgtgacgat cagattcctg     1860

gatcctccgc ttcatgagtt cctgccccag gaggatgaag acatagaagc cttggcaaaa     1920

gaaatgggaa ttactttcga tgaactgaaa gcaatagtaa ccgggcttca tgagttcaat     1980

cctatgatgg gacacagggg atgccgtctt gcagtcacat atccggaaat tgcggaaatg     2040

cagacgagag cggttattga agctgctatc aacgtgagca ggaagaatat aaaagttgtg     2100

cctgaaatta tgattccgtt ggtaggcgat gtcaaggagc tgaaatatgt caaggacgta     2160

gttgtcagaa cagccaatga attgattgaa aaatccggtg tgaagattga atataaagtc     2220

ggaaccatga tagaaattcc aagggcggcc attactgccg atgaaattgc aaaagaagct     2280

gaattcttct cctttggaac caacgacctg acccagatga cttttggatt cagccgtgac     2340

gatgcaggca agttccttga agaatactac aacaagaaga tatacgagtt cgatcctttt     2400

gcaaaactgg atcaggatgg agtggggaaa ctggttgaaa tggctgcgaa gcttggaaga     2460

caaacaagac cggatattaa gcttggtata tgcggtgaac atggcggaga tccgtcgtcc     2520

attgagttct gccaccaaat tgggctgaac tatgtatcat gctctccgtt ccgtgtgccg     2580

attgcaaggc ttgcagcggc tcaggcaaga gtaaatgaaa taaaaggtac aaaggatttg     2640

ggacagaaat aa                                                         2652


<210>  42
<211>  883
<212>  PRT
<213>  C.thermocellum pyruvate-phosphate dikinase

<400>  42

Met Ala Lys Tyr Val Tyr Leu Phe Ser Glu Gly Asn Ala Ser Met Arg 
1               5                   10                  15      


Asp Leu Leu Gly Gly Lys Gly Ala Asn Leu Ala Glu Met Thr Ser Leu 
            20                  25                  30          


Gly Leu Pro Val Pro Arg Gly Phe Thr Ile Thr Thr Glu Ala Cys Thr 
        35                  40                  45              


Arg Tyr Tyr Gln Asp Gly Lys Val Ile Ala Lys Glu Ile Glu Asp Glu 
    50                  55                  60                  


Ile Tyr Arg Thr Met Glu Lys Leu Glu Glu Ile Val Gly Lys Lys Phe 
65                  70                  75                  80  


Gly Asp Pro Ser Asn Pro Phe Leu Val Ser Val Arg Ser Gly Ala Arg 
                85                  90                  95      


Val Ser Met Pro Gly Met Met Asp Thr Ile Leu Asn Leu Gly Leu Asn 
            100                 105                 110         


Asp Glu Val Val Val Gly Leu Ala Lys Leu Thr Asn Asn Glu Arg Phe 
        115                 120                 125             


Ala Tyr Asp Ser Tyr Arg Arg Phe Ile Gln Met Phe Ser Asp Val Val 
    130                 135                 140                 


Met Glu Val Glu Lys Ser Lys Phe Glu Ala Ile Leu Asp Ala Val Lys 
145                 150                 155                 160 


Glu Glu Asn Asn Cys Glu Asn Asp Cys Asp Leu Ser Ala Glu Asn Leu 
                165                 170                 175     


Lys Glu Val Val Arg Arg Tyr Lys Glu Leu Phe Lys Lys Glu Lys Gly 
            180                 185                 190         


Phe Asp Phe Pro Gln Asp Pro Lys Thr Gln Leu Met Glu Ala Val Lys 
        195                 200                 205             


Ala Val Phe Arg Ser Trp Glu Asn Pro Arg Ala Ile Val Tyr Arg Arg 
    210                 215                 220                 


Leu Asn Asp Ile Pro Gly Asp Trp Gly Thr Ala Val Asn Val Gln Glu 
225                 230                 235                 240 


Met Val Tyr Gly Asn Met Gly Asn Asp Ser Gly Thr Gly Val Ala Phe 
                245                 250                 255     


Thr Arg Asn Pro Ala Thr Gly Glu Lys Lys Leu Tyr Gly Glu Phe Leu 
            260                 265                 270         


Met Asn Ala Gln Gly Glu Asp Val Val Ala Gly Ile Arg Thr Pro Gln 
        275                 280                 285             


Ser Ile Asp Gln Leu Lys Glu Val Met Pro Asp Val Tyr Asn Gln Phe 
    290                 295                 300                 


Val Glu Ile Ala Glu Lys Leu Glu Arg His Tyr Arg Asp Met Gln Asp 
305                 310                 315                 320 


Met Glu Phe Thr Ile Glu Arg Gly Lys Leu Phe Met Leu Gln Thr Arg 
                325                 330                 335     


Asn Gly Lys Arg Thr Ala Ala Ala Ala Leu Lys Ile Ala Val Asp Leu 
            340                 345                 350         


Val Asn Glu Gly Met Val Thr Lys Glu Glu Ala Ile Leu Lys Val Asp 
        355                 360                 365             


Pro Lys Gln Leu Asp Thr Leu Leu His Pro Asn Phe Glu Pro Ser Ala 
    370                 375                 380                 


Leu Lys Asn Ala Lys Pro Ile Ala Lys Gly Leu Pro Ala Ser Pro Gly 
385                 390                 395                 400 


Ala Ala Thr Gly Lys Ile Tyr Phe Arg Ala Glu Asp Ala Val Glu Ala 
                405                 410                 415     


Ala Lys Asn Gly Glu Lys Asp Ile Ile Leu Val Arg Leu Glu Thr Ser 
            420                 425                 430         


Pro Glu Asp Ile Glu Gly Met His Val Ser Lys Gly Ile Leu Thr Gly 
        435                 440                 445             


Arg Gly Gly Met Thr Ser His Ala Ala Val Val Ala Arg Gly Met Gly 
    450                 455                 460                 


Thr Cys Cys Val Ala Gly Cys Ser Glu Ile Arg Ile Asn Glu Glu Glu 
465                 470                 475                 480 


Lys Tyr Phe Val Asp Lys Asn Gly Lys Lys Tyr Val Glu Gly Asp Trp 
                485                 490                 495     


Ile Ser Leu Asp Gly Ser Thr Gly Asn Val Tyr Gly Glu Lys Leu Pro 
            500                 505                 510         


Thr Val Glu Pro Glu Met Thr Gly Asp Phe Ala Thr Leu Met Gln Trp 
        515                 520                 525             


Ala Asp Glu Ile Arg Thr Leu Lys Ile Arg Thr Asn Ala Asp Thr Pro 
    530                 535                 540                 


Ala Asp Ala Ile Gln Ala Arg Lys Phe Gly Ala Glu Gly Ile Gly Leu 
545                 550                 555                 560 


Cys Arg Thr Glu His Met Phe Phe Asp Ser Asp Arg Ile Pro Ala Met 
                565                 570                 575     


Arg Glu Met Ile Val Ala Arg Thr Glu Glu Gln Arg Arg Lys Ala Leu 
            580                 585                 590         


Asp Lys Leu Leu Pro Met Gln Arg Lys Asp Phe Glu Glu Leu Phe Thr 
        595                 600                 605             


Ala Met Glu Gly Tyr Pro Val Thr Ile Arg Phe Leu Asp Pro Pro Leu 
    610                 615                 620                 


His Glu Phe Leu Pro Gln Glu Asp Glu Asp Ile Glu Ala Leu Ala Lys 
625                 630                 635                 640 


Glu Met Gly Ile Thr Phe Asp Glu Leu Lys Ala Ile Val Thr Gly Leu 
                645                 650                 655     


His Glu Phe Asn Pro Met Met Gly His Arg Gly Cys Arg Leu Ala Val 
            660                 665                 670         


Thr Tyr Pro Glu Ile Ala Glu Met Gln Thr Arg Ala Val Ile Glu Ala 
        675                 680                 685             


Ala Ile Asn Val Ser Arg Lys Asn Ile Lys Val Val Pro Glu Ile Met 
    690                 695                 700                 


Ile Pro Leu Val Gly Asp Val Lys Glu Leu Lys Tyr Val Lys Asp Val 
705                 710                 715                 720 


Val Val Arg Thr Ala Asn Glu Leu Ile Glu Lys Ser Gly Val Lys Ile 
                725                 730                 735     


Glu Tyr Lys Val Gly Thr Met Ile Glu Ile Pro Arg Ala Ala Ile Thr 
            740                 745                 750         


Ala Asp Glu Ile Ala Lys Glu Ala Glu Phe Phe Ser Phe Gly Thr Asn 
        755                 760                 765             


Asp Leu Thr Gln Met Thr Phe Gly Phe Ser Arg Asp Asp Ala Gly Lys 
    770                 775                 780                 


Phe Leu Glu Glu Tyr Tyr Asn Lys Lys Ile Tyr Glu Phe Asp Pro Phe 
785                 790                 795                 800 


Ala Lys Leu Asp Gln Asp Gly Val Gly Lys Leu Val Glu Met Ala Ala 
                805                 810                 815     


Lys Leu Gly Arg Gln Thr Arg Pro Asp Ile Lys Leu Gly Ile Cys Gly 
            820                 825                 830         


Glu His Gly Gly Asp Pro Ser Ser Ile Glu Phe Cys His Gln Ile Gly 
        835                 840                 845             


Leu Asn Tyr Val Ser Cys Ser Pro Phe Arg Val Pro Ile Ala Arg Leu 
    850                 855                 860                 


Ala Ala Ala Gln Ala Arg Val Asn Glu Ile Lys Gly Thr Lys Asp Leu 
865                 870                 875                 880 


Gly Gln Lys 
            


<210>  43
<211>  1818
<212>  DNA
<213>  C.thermocellum phosphoenolpyruvate carboxykinase

<400>  43
atgacatcaa caaacatgac aaaaaacaaa aaactgctgg attgggttaa ggaaatggct       60

gaaatgtgtc agcctgatga aatttattgg tgcgatggtt cggaggaaga aaatgagcgc      120

ttgataaagt tgatggtgga ttcaggtttg gctacgcctt tgaatcctga aaagcgacct      180

ggatgttatc tcttccgcag cgatccgtcc gacgttgccc gtgttgagga cagaactttt      240

attgcatcca aaaccaaaga agatgcagga cctacaaaca actggataga tccggttgag      300

ctcaaggcaa ctatgaaaga gttgtacaag ggttgtatga agggaagaac aatgtatgtt      360

attcctttct ccatgggacc tatcggttca cccatttcaa aaatcggcgt tgaattgacc      420

gacagccctt atgttgttgt taacatgcgc attatgactc gcataggcaa ggctgtgttg      480

gatcagctcg gagaagacgg agattttgta ccttgtctcc actcagtcgg tgctccgctc      540

aaagagggag aaaaggataa aggttggcca tgcgcaccaa tcgaaaagaa atacataagc      600

cacttcccgg aagaaaggac tatatggtca tatggttccg gatacggtgg aaatgcgctt      660

ttaggaaaga aatgctttgc acttcgtatt gcatctgtta tggcacgtga cgaaggttgg      720

cttgctgaac acatgcttat ccttcgcata acagaccctg aaggaaacaa gacatatgtt      780

acaggtgctt tcccaagcgc atgcggaaag acgaacctgg ctatgcttat tcctacaatt      840

cccggatgga aagttgaaac aatcggtgac gatattgcat ggatgagatt tggaaaagac      900

ggccgtttgt atgctatcaa ccctgaagca ggattctttg gtgttgctcc gggtacatcc      960

atggattcaa atccgaacgc aatgcataca attaagaaaa atactatatt tacaaacgtt     1020

gcattgactg atgacggcga tgtttggtgg gaaggcatcg gaactgaacc gccggctcat     1080

ctcatagact ggcagggtaa agactggact cctgattccg gaactttggc agcacatccc     1140

aacggacgtt ttacagcacc tgcaagtcag tgccctgtaa ttgctcctga atgggaggat     1200

ccggaaggtg tgccgatttc agcaatcctt atcggtggac gccgtccgaa caccattccg     1260

cttgttcatg aaagctttga ctggaaccat ggtgtattca tgggttcaat catgggttct     1320

gaaattacgg ctgccgcaat ttcaaacaaa atcggacagg tacgccgtga cccgtttgct     1380

atgctgcctt tcataggcta caacgtaaat gactatttgc agcactggtt gaacatgggt     1440

accaagactg acccaagcaa gcttcccaag atattctatg taaactggtt ccgcaaggac     1500

agcaacggta aatggttgtg gcctggatac ggtgaaaaca gccgtgttct caagtggatt     1560

gttgaaagag tcaacggaaa aggtaaagca gtaaagacac ctataggata tatgcctaca     1620

gttgacgcta tcgacacaac cggccttgat gtaagcaaag aggatatgga agaactcttg     1680

agcgttaaca aagaacagtg gctccaggaa gttgagtcaa taaaagaaca ttataagtca     1740

tacggagaaa aactgccgaa agaattgtgg gcacaattgg aggctcttga acaacgtttg     1800

aaagagtata acggttaa                                                   1818


<210>  44
<211>  605
<212>  PRT
<213>  C.thermocellum phosphoenolpyruvate carboxykinase

<400>  44

Met Thr Ser Thr Asn Met Thr Lys Asn Lys Lys Leu Leu Asp Trp Val 
1               5                   10                  15      


Lys Glu Met Ala Glu Met Cys Gln Pro Asp Glu Ile Tyr Trp Cys Asp 
            20                  25                  30          


Gly Ser Glu Glu Glu Asn Glu Arg Leu Ile Lys Leu Met Val Asp Ser 
        35                  40                  45              


Gly Leu Ala Thr Pro Leu Asn Pro Glu Lys Arg Pro Gly Cys Tyr Leu 
    50                  55                  60                  


Phe Arg Ser Asp Pro Ser Asp Val Ala Arg Val Glu Asp Arg Thr Phe 
65                  70                  75                  80  


Ile Ala Ser Lys Thr Lys Glu Asp Ala Gly Pro Thr Asn Asn Trp Ile 
                85                  90                  95      


Asp Pro Val Glu Leu Lys Ala Thr Met Lys Glu Leu Tyr Lys Gly Cys 
            100                 105                 110         


Met Lys Gly Arg Thr Met Tyr Val Ile Pro Phe Ser Met Gly Pro Ile 
        115                 120                 125             


Gly Ser Pro Ile Ser Lys Ile Gly Val Glu Leu Thr Asp Ser Pro Tyr 
    130                 135                 140                 


Val Val Val Asn Met Arg Ile Met Thr Arg Ile Gly Lys Ala Val Leu 
145                 150                 155                 160 


Asp Gln Leu Gly Glu Asp Gly Asp Phe Val Pro Cys Leu His Ser Val 
                165                 170                 175     


Gly Ala Pro Leu Lys Glu Gly Glu Lys Asp Lys Gly Trp Pro Cys Ala 
            180                 185                 190         


Pro Ile Glu Lys Lys Tyr Ile Ser His Phe Pro Glu Glu Arg Thr Ile 
        195                 200                 205             


Trp Ser Tyr Gly Ser Gly Tyr Gly Gly Asn Ala Leu Leu Gly Lys Lys 
    210                 215                 220                 


Cys Phe Ala Leu Arg Ile Ala Ser Val Met Ala Arg Asp Glu Gly Trp 
225                 230                 235                 240 


Leu Ala Glu His Met Leu Ile Leu Arg Ile Thr Asp Pro Glu Gly Asn 
                245                 250                 255     


Lys Thr Tyr Val Thr Gly Ala Phe Pro Ser Ala Cys Gly Lys Thr Asn 
            260                 265                 270         


Leu Ala Met Leu Ile Pro Thr Ile Pro Gly Trp Lys Val Glu Thr Ile 
        275                 280                 285             


Gly Asp Asp Ile Ala Trp Met Arg Phe Gly Lys Asp Gly Arg Leu Tyr 
    290                 295                 300                 


Ala Ile Asn Pro Glu Ala Gly Phe Phe Gly Val Ala Pro Gly Thr Ser 
305                 310                 315                 320 


Met Asp Ser Asn Pro Asn Ala Met His Thr Ile Lys Lys Asn Thr Ile 
                325                 330                 335     


Phe Thr Asn Val Ala Leu Thr Asp Asp Gly Asp Val Trp Trp Glu Gly 
            340                 345                 350         


Ile Gly Thr Glu Pro Pro Ala His Leu Ile Asp Trp Gln Gly Lys Asp 
        355                 360                 365             


Trp Thr Pro Asp Ser Gly Thr Leu Ala Ala His Pro Asn Gly Arg Phe 
    370                 375                 380                 


Thr Ala Pro Ala Ser Gln Cys Pro Val Ile Ala Pro Glu Trp Glu Asp 
385                 390                 395                 400 


Pro Glu Gly Val Pro Ile Ser Ala Ile Leu Ile Gly Gly Arg Arg Pro 
                405                 410                 415     


Asn Thr Ile Pro Leu Val His Glu Ser Phe Asp Trp Asn His Gly Val 
            420                 425                 430         


Phe Met Gly Ser Ile Met Gly Ser Glu Ile Thr Ala Ala Ala Ile Ser 
        435                 440                 445             


Asn Lys Ile Gly Gln Val Arg Arg Asp Pro Phe Ala Met Leu Pro Phe 
    450                 455                 460                 


Ile Gly Tyr Asn Val Asn Asp Tyr Leu Gln His Trp Leu Asn Met Gly 
465                 470                 475                 480 


Thr Lys Thr Asp Pro Ser Lys Leu Pro Lys Ile Phe Tyr Val Asn Trp 
                485                 490                 495     


Phe Arg Lys Asp Ser Asn Gly Lys Trp Leu Trp Pro Gly Tyr Gly Glu 
            500                 505                 510         


Asn Ser Arg Val Leu Lys Trp Ile Val Glu Arg Val Asn Gly Lys Gly 
        515                 520                 525             


Lys Ala Val Lys Thr Pro Ile Gly Tyr Met Pro Thr Val Asp Ala Ile 
    530                 535                 540                 


Asp Thr Thr Gly Leu Asp Val Ser Lys Glu Asp Met Glu Glu Leu Leu 
545                 550                 555                 560 


Ser Val Asn Lys Glu Gln Trp Leu Gln Glu Val Glu Ser Ile Lys Glu 
                565                 570                 575     


His Tyr Lys Ser Tyr Gly Glu Lys Leu Pro Lys Glu Leu Trp Ala Gln 
            580                 585                 590         


Leu Glu Ala Leu Glu Gln Arg Leu Lys Glu Tyr Asn Gly 
        595                 600                 605 


<210>  45
<211>  954
<212>  DNA
<213>  C.thermocellum lactate dehydrogenase

<400>  45
atgaacaata acaaagtaat taaaaaagta accgtagttg gtgcaggctt tgtaggttcc       60

accacagctt atacattgat gctcagcgga cttatatctg aaattgtact gatagacata      120

aatgcaaaaa aagccgacgg agaagtcatg gacttaaatc acggcatgcc ttttgtaagg      180

cccgttgaaa tttatcgtgg tgactacaaa gactgtgccg gatccgacat agtaatcatt      240

accgccggtg ccaaccaaaa agaaggcgaa acgagaatag atcttgttaa aagaaacacg      300

gaagtattca aaaatatcat aaatgaaatt gtaaagtaca acaacgattg tattcttctg      360

gtagtcacaa atccggtgga tattttaacc tatgtaactt acaaactatc cggattcccg      420

aaaaacaaag taataggttc cggaacggtt ttggacacag ccaggttccg ttatctttta      480

agcgaacatg taaaagtgga tgcacgaaat gtacatgctt atattattgg cgaacacggt      540

gacaccgaag ttgcggcctg gagtcttgca aatattgcgg gaattcccat ggatcgctac      600

tgtgacgaat gccatcagtg cgaggagcag atttcccgga ataaaatata tgaaagtgtt      660

aaaaatgcag cttatgaaat catcaggaac aaaggtgcaa cctattatgc cgtagccctt      720

gccgtaagaa gaatcgttga agccattgta agaaatgaaa actccatcct taccgtttca      780

agccttttgg aaggacagta cggacttagc gatgtatgct taagtgttcc gacaatcgtg      840

ggtgtaaacg gtattgagga aatattgaac gtgcctttca acgatgaaga aattcagctt      900

ctcagaaagt ccggaaacac tctaaaagaa ataataaaaa cactagatat atga            954


<210>  46
<211>  317
<212>  PRT
<213>  C.thermocellum lactate dehydrogenase

<400>  46

Met Asn Asn Asn Lys Val Ile Lys Lys Val Thr Val Val Gly Ala Gly 
1               5                   10                  15      


Phe Val Gly Ser Thr Thr Ala Tyr Thr Leu Met Leu Ser Gly Leu Ile 
            20                  25                  30          


Ser Glu Ile Val Leu Ile Asp Ile Asn Ala Lys Lys Ala Asp Gly Glu 
        35                  40                  45              


Val Met Asp Leu Asn His Gly Met Pro Phe Val Arg Pro Val Glu Ile 
    50                  55                  60                  


Tyr Arg Gly Asp Tyr Lys Asp Cys Ala Gly Ser Asp Ile Val Ile Ile 
65                  70                  75                  80  


Thr Ala Gly Ala Asn Gln Lys Glu Gly Glu Thr Arg Ile Asp Leu Val 
                85                  90                  95      


Lys Arg Asn Thr Glu Val Phe Lys Asn Ile Ile Asn Glu Ile Val Lys 
            100                 105                 110         


Tyr Asn Asn Asp Cys Ile Leu Leu Val Val Thr Asn Pro Val Asp Ile 
        115                 120                 125             


Leu Thr Tyr Val Thr Tyr Lys Leu Ser Gly Phe Pro Lys Asn Lys Val 
    130                 135                 140                 


Ile Gly Ser Gly Thr Val Leu Asp Thr Ala Arg Phe Arg Tyr Leu Leu 
145                 150                 155                 160 


Ser Glu His Val Lys Val Asp Ala Arg Asn Val His Ala Tyr Ile Ile 
                165                 170                 175     


Gly Glu His Gly Asp Thr Glu Val Ala Ala Trp Ser Leu Ala Asn Ile 
            180                 185                 190         


Ala Gly Ile Pro Met Asp Arg Tyr Cys Asp Glu Cys His Gln Cys Glu 
        195                 200                 205             


Glu Gln Ile Ser Arg Asn Lys Ile Tyr Glu Ser Val Lys Asn Ala Ala 
    210                 215                 220                 


Tyr Glu Ile Ile Arg Asn Lys Gly Ala Thr Tyr Tyr Ala Val Ala Leu 
225                 230                 235                 240 


Ala Val Arg Arg Ile Val Glu Ala Ile Val Arg Asn Glu Asn Ser Ile 
                245                 250                 255     


Leu Thr Val Ser Ser Leu Leu Glu Gly Gln Tyr Gly Leu Ser Asp Val 
            260                 265                 270         


Cys Leu Ser Val Pro Thr Ile Val Gly Val Asn Gly Ile Glu Glu Ile 
        275                 280                 285             


Leu Asn Val Pro Phe Asn Asp Glu Glu Ile Gln Leu Leu Arg Lys Ser 
    290                 295                 300                 


Gly Asn Thr Leu Lys Glu Ile Ile Lys Thr Leu Asp Ile 
305                 310                 315         


<210>  47
<211>  1077
<212>  DNA
<213>  C.thermocellum phosphotransacetylase

<400>  47
gtgataatat atagttataa gtattacaaa tacagttttt atgataacag ttttgggatt       60

atgaaaggag aagaatttat gagttttttg gaacaaataa ttgaaagggc gaaatcagac      120

gtaaaaacca tagttttgcc ggaaagtacg gatctgaggg ttattaaagc cgcatccatg      180

atagtgaaaa agggaattgc aaaggttgta ctgataggca atgaaaagga gattaagagt      240

ctggcggggg atattgatct tgaaggagtg atgatagagg attccttaaa ttccgaaaaa      300

ttggaggatt atgcaaatac actgtatgag cttagaaaat cgaagggtat gactatagaa      360

gccgcaaggg aaacgatcaa agaccctctt tattatggag ttatgatggt aaaaaaaggt      420

gaagcggatg gtatggtggc gggtgctgtc aattccactg caaatacttt gagaccggct      480

ttgcagatat taaagacggc cccggggaca aaactcgtat catccttttt tgttatggtt      540

gtacccaact gtgaatatgg tcataacgga acctttgtat atgccgattg cggcttggtg      600

gaaaatccgg atgcagacca gctttctgaa attgcaatat ctgcatccaa atcttttgag      660

atgctggttg gagcaaaacc tcaggtggca atgctttctt attcttctta cggcagtgcc      720

aaaagtgagc tgaccgaaaa ggtaatcaag gcaacacagc ttgcaaagga aaaagctccc      780

caccttgcaa ttgacggaga acttcaggtg gatgccgcca ttgttccgga agtggcaaaa      840

tcgaaggcaa agggaagcag tgttgcagga aaggccaatg ttcttatttt cccggatctt      900

gatgccggaa atattgcata caagcttaca cagagattgg caaaagctga agcttacggc      960

ccgataacac aaggtttggc aagaccggta aatgagctgt cacgaggctg cagtgccgag     1020

gatatagtcg gggttgcggc aattactgcg gttcaggctc aatatgtcaa ggcataa        1077


<210>  48
<211>  358
<212>  PRT
<213>  C.thermocellum phosphotransacetylase

<400>  48

Val Ile Ile Tyr Ser Tyr Lys Tyr Tyr Lys Tyr Ser Phe Tyr Asp Asn 
1               5                   10                  15      


Ser Phe Gly Ile Met Lys Gly Glu Glu Phe Met Ser Phe Leu Glu Gln 
            20                  25                  30          


Ile Ile Glu Arg Ala Lys Ser Asp Val Lys Thr Ile Val Leu Pro Glu 
        35                  40                  45              


Ser Thr Asp Leu Arg Val Ile Lys Ala Ala Ser Met Ile Val Lys Lys 
    50                  55                  60                  


Gly Ile Ala Lys Val Val Leu Ile Gly Asn Glu Lys Glu Ile Lys Ser 
65                  70                  75                  80  


Leu Ala Gly Asp Ile Asp Leu Glu Gly Val Met Ile Glu Asp Ser Leu 
                85                  90                  95      


Asn Ser Glu Lys Leu Glu Asp Tyr Ala Asn Thr Leu Tyr Glu Leu Arg 
            100                 105                 110         


Lys Ser Lys Gly Met Thr Ile Glu Ala Ala Arg Glu Thr Ile Lys Asp 
        115                 120                 125             


Pro Leu Tyr Tyr Gly Val Met Met Val Lys Lys Gly Glu Ala Asp Gly 
    130                 135                 140                 


Met Val Ala Gly Ala Val Asn Ser Thr Ala Asn Thr Leu Arg Pro Ala 
145                 150                 155                 160 


Leu Gln Ile Leu Lys Thr Ala Pro Gly Thr Lys Leu Val Ser Ser Phe 
                165                 170                 175     


Phe Val Met Val Val Pro Asn Cys Glu Tyr Gly His Asn Gly Thr Phe 
            180                 185                 190         


Val Tyr Ala Asp Cys Gly Leu Val Glu Asn Pro Asp Ala Asp Gln Leu 
        195                 200                 205             


Ser Glu Ile Ala Ile Ser Ala Ser Lys Ser Phe Glu Met Leu Val Gly 
    210                 215                 220                 


Ala Lys Pro Gln Val Ala Met Leu Ser Tyr Ser Ser Tyr Gly Ser Ala 
225                 230                 235                 240 


Lys Ser Glu Leu Thr Glu Lys Val Ile Lys Ala Thr Gln Leu Ala Lys 
                245                 250                 255     


Glu Lys Ala Pro His Leu Ala Ile Asp Gly Glu Leu Gln Val Asp Ala 
            260                 265                 270         


Ala Ile Val Pro Glu Val Ala Lys Ser Lys Ala Lys Gly Ser Ser Val 
        275                 280                 285             


Ala Gly Lys Ala Asn Val Leu Ile Phe Pro Asp Leu Asp Ala Gly Asn 
    290                 295                 300                 


Ile Ala Tyr Lys Leu Thr Gln Arg Leu Ala Lys Ala Glu Ala Tyr Gly 
305                 310                 315                 320 


Pro Ile Thr Gln Gly Leu Ala Arg Pro Val Asn Glu Leu Ser Arg Gly 
                325                 330                 335     


Cys Ser Ala Glu Asp Ile Val Gly Val Ala Ala Ile Thr Ala Val Gln 
            340                 345                 350         


Ala Gln Tyr Val Lys Ala 
        355             


<210>  49
<211>  1200
<212>  DNA
<213>  C.thermocellum acetate kinase

<400>  49
atgaatattt tggttattaa taccggaagc tcatcactaa agtatcagct gattgacatg       60

acaaacgagt ctgtgcttgc aaaaggtgtg tgtgacagaa ttggtcttga acattccttt      120

ttaaagcata caaagaccgg aggggaaacc gtagttatag aaaaagacct gtacaatcac      180

aagcttgcca tacaggaggt aatttcggct cttacggatg aaaaaatcgg agtcataaaa      240

agcatgtcgg aaatttctgc cgtcggtcat cgtattgttc acggcggaga gaagtttaag      300

gaatctgcca taattgatga agatgtaatg aaagcaatca gggattgtgt tgaactggct      360

ccgctccaca atccgtcaaa tataatcgga attgaagcct gtaaacagat actgcccgat      420

gtgccgatgg ttgctgtgtt tgacacagct tttcatcaga caatgccaag gcatgcatat      480

atttatgccc tcccttatga gatatatgag aagtataaat tgagaaaata cggattccac      540

ggaacttccc acaaatatgt ggcccacagg gcggctcaga tgctgggcaa acctattgag      600

agcctgaagc tgataacctg ccatcttgga aacggagcga gtatttgtgc ggtaaaaggc      660

ggaaaatccg ttgacacctc aatgggattt actcctctgc aggggttgtg catgggtacc      720

agaagcggca atgttgaccc tgcggttata acttatttga tggaaaagga aaaaatgaat      780

attaacgata taaacaattt ccttaacaag aaatcaggtg tgcttggaat ttcaggtgta      840

agcagtgatt tcagagatgt tcaggatgcc gcagaaaagg gagatgacag ggcgcagctg      900

gcattggata ttttctgcta tggtgttagg aaatatattg gaaaatatat tgcagtgctg      960

aacggcgttg atgcggtggt attcactgca ggtatcggcg aaaacaatgc ttatataaga     1020

agagaagttt tgaaggatat ggactttttc ggaattaaaa tagatttgga taaaaatgaa     1080

gtgaaaggca aagaagcgga tatcagtgct cccgatgcga aagtaaagac tttggttatc     1140

ccgacaaatg aggagcttga gattgcaagg gagactttaa gacttgtaaa aaacttataa     1200


<210>  50
<211>  399
<212>  PRT
<213>  C.thermocellum acetate kinase

<400>  50

Met Asn Ile Leu Val Ile Asn Thr Gly Ser Ser Ser Leu Lys Tyr Gln 
1               5                   10                  15      


Leu Ile Asp Met Thr Asn Glu Ser Val Leu Ala Lys Gly Val Cys Asp 
            20                  25                  30          


Arg Ile Gly Leu Glu His Ser Phe Leu Lys His Thr Lys Thr Gly Gly 
        35                  40                  45              


Glu Thr Val Val Ile Glu Lys Asp Leu Tyr Asn His Lys Leu Ala Ile 
    50                  55                  60                  


Gln Glu Val Ile Ser Ala Leu Thr Asp Glu Lys Ile Gly Val Ile Lys 
65                  70                  75                  80  


Ser Met Ser Glu Ile Ser Ala Val Gly His Arg Ile Val His Gly Gly 
                85                  90                  95      


Glu Lys Phe Lys Glu Ser Ala Ile Ile Asp Glu Asp Val Met Lys Ala 
            100                 105                 110         


Ile Arg Asp Cys Val Glu Leu Ala Pro Leu His Asn Pro Ser Asn Ile 
        115                 120                 125             


Ile Gly Ile Glu Ala Cys Lys Gln Ile Leu Pro Asp Val Pro Met Val 
    130                 135                 140                 


Ala Val Phe Asp Thr Ala Phe His Gln Thr Met Pro Arg His Ala Tyr 
145                 150                 155                 160 


Ile Tyr Ala Leu Pro Tyr Glu Ile Tyr Glu Lys Tyr Lys Leu Arg Lys 
                165                 170                 175     


Tyr Gly Phe His Gly Thr Ser His Lys Tyr Val Ala His Arg Ala Ala 
            180                 185                 190         


Gln Met Leu Gly Lys Pro Ile Glu Ser Leu Lys Leu Ile Thr Cys His 
        195                 200                 205             


Leu Gly Asn Gly Ala Ser Ile Cys Ala Val Lys Gly Gly Lys Ser Val 
    210                 215                 220                 


Asp Thr Ser Met Gly Phe Thr Pro Leu Gln Gly Leu Cys Met Gly Thr 
225                 230                 235                 240 


Arg Ser Gly Asn Val Asp Pro Ala Val Ile Thr Tyr Leu Met Glu Lys 
                245                 250                 255     


Glu Lys Met Asn Ile Asn Asp Ile Asn Asn Phe Leu Asn Lys Lys Ser 
            260                 265                 270         


Gly Val Leu Gly Ile Ser Gly Val Ser Ser Asp Phe Arg Asp Val Gln 
        275                 280                 285             


Asp Ala Ala Glu Lys Gly Asp Asp Arg Ala Gln Leu Ala Leu Asp Ile 
    290                 295                 300                 


Phe Cys Tyr Gly Val Arg Lys Tyr Ile Gly Lys Tyr Ile Ala Val Leu 
305                 310                 315                 320 


Asn Gly Val Asp Ala Val Val Phe Thr Ala Gly Ile Gly Glu Asn Asn 
                325                 330                 335     


Ala Tyr Ile Arg Arg Glu Val Leu Lys Asp Met Asp Phe Phe Gly Ile 
            340                 345                 350         


Lys Ile Asp Leu Asp Lys Asn Glu Val Lys Gly Lys Glu Ala Asp Ile 
        355                 360                 365             


Ser Ala Pro Asp Ala Lys Val Lys Thr Leu Val Ile Pro Thr Asn Glu 
    370                 375                 380                 


Glu Leu Glu Ile Ala Arg Glu Thr Leu Arg Leu Val Lys Asn Leu 
385                 390                 395                 


<210>  51
<211>  56
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  51
aaaataagct atatgaaggg agaatggaga tgaacaatag acaacccctt tctgtg           56


<210>  52
<211>  6259
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pAMG206::Pcbp-Mj_mdh

<400>  52
gagtcgtgac taagaacgtc aaagtaatta acaatacagc tatttttctc atgcttttac       60

ccctttcata aaatttaatt ttatcgttat cataaaaaat tatagacgtt atattgcttg      120

ccgggatata gtgctgggca ttcgttggtg caaaatgttc ggagtaaggt ggatattgat      180

ttgcatgttg atctattgca ttgaaatgat tagttatccg taaatattaa ttaatcatat      240

cataaattaa ttatatcata attgttttga cgaatgaagg tttttggata aattatcaag      300

taaaggaacg ctaaaaattt tggcgtaaaa tatcaaaatg accacttgaa ttaatatggt      360

aaagtagata taatattttg gtaaacatgc cttcagcaag gttagattag ctgtttccgt      420

ataaattaac cgtatggtaa aacggcagtc agaaaaataa gtcataagat tccgttatga      480

aaatatactt cggtagttaa taataagaga tatgaggtaa gagatacaag ataagagata      540

taaggtacga atgtataaga tggtgctttt aggcacacta aataaaaaac aaataaacga      600

aaattttaag gaggacgaaa gatgaaagtt acaattatag gagcttctgg tagagttggg      660

agtgcaacag ccttattatt agctaaagaa ccttttatga aggatttggt gttaattgga      720

agagaacatt caataaataa attggaagga ttgagagaag acatctacga tgccttagct      780

gggacaagaa gtgatgcaaa tatatacgtt gagagtgatg aaaatctaag gataattgat      840

gaaagtgatg ttgttataat aacaagcggt gttccaagaa aagagggaat gagtaggatg      900

gatttggcaa aaacaaatgc aaaaattgtt gggaagtatg ctaaaaaaat agctgaaatc      960

tgcgatacaa aaatatttgt tataacaaac cctgtggatg tgatgactta taaagctctg     1020

gtagattcaa aatttgaaag aaatcaagtt tttggattag ggactcattt agattctttg     1080

aggtttaagg ttgctattgc taagtttttc ggtgttcata ttgatgaagt taggacgaga     1140

attattggag agcatgggga cagcatggtt ccattgttaa gtgctacctc tatcggagga     1200

attcctattc aaaaatttga aagatttaag gaactgccaa tagatgagat tatagaggat     1260

gttaaaacaa aaggagagca gattattaga ttgaaaggag gttctgagtt tggtccagca     1320

gcagccattt taaatgttgt taggtgtatt gtgaataatg agaaaagatt gctaacttta     1380

tccgcttacg tagatggaga gtttgatgga attagagatg tgtgtattgg agttccagta     1440

aagattggaa gagatgggat agaagaggtt gtatcaattg aattggataa agatgagata     1500

attgcattta gaaaatctgc tgaaatcatt aaaaaatact gtgaagaagt taaaaactta     1560

taaaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag     1620

atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa     1680

aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg     1740

aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag     1800

ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg     1860

ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga     1920

tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc     1980

ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc     2040

acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga     2100

gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt     2160

cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg     2220

aaaaacgcca gcaacgcggc ctttttacgg ttcctgggct tttgctggcc ttttgctcac     2280

atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga     2340

gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg     2400

gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagg     2460

gccgcagctt gcaaattaaa gccttcgagc gtcccaaaac cttctcaagc aaggttttca     2520

gtataatgtt acatgcgtac acgcgtctgt acagaaaaaa aagaaaaatt tgaaatataa     2580

ataacgttct taatactaac ataactataa aaaaataaat agggacctag acttcaggtt     2640

gtctaactcc ttccttttcg gttagagcgg atgtgggggg agggcgtgaa tgtaagcgtg     2700

acataactaa ttacatgatg cggccctcta ggatcagcgg gtttaaacgc tgaggcgcgc     2760

cgaattcggt accaaaacaa aaggcccagt cttccgactg agccttttgt tttctcgagg     2820

cccgggctta gctgtacatt tcaggtttca aaacgcctat aaaaggaagg ttcctgtact     2880

tttcatcaaa gtccaatcca taacccacga caaacttatc aggtatctta aatcctacat     2940

aatcgacttt tacagatgct tctcttctct ccggtttgtc taatattgtg catattttca     3000

ggctttttgg cttccttcca agtaaagttt cccttaagta agacaaagtc aaaccgctgt     3060

caattatgtc ttccacaatc agaacatctt tgccttctat gcttatatca agatctttga     3120

ttatctttac tattcctgat gaatgagttg agcttccata gctggaaaca gccataaaat     3180

caagtgataa aggcaaatct atgtgtcttg acaaatcagc cataaacatt aaagcacctt     3240

ttaaaactcc taccaacatc aaatttttcc ctttgtagtc ttttgtgatt tgcctcccaa     3300

gctcttttat cttttcctta agttcttctt ctgtgatcaa aatttcatcg atgtcttttg     3360

ataaattttc catgacgtcg acctccttta ttataaaagc cagtcattag gcctatctga     3420

caattcctga atagagttca taaacaatcc tgcatgataa ccatcacaaa cagaatgatg     3480

tacctgtaaa gatagcggta aatatattga attaccttta ttaatgaatt ttcctgctgt     3540

aataatgggt agaaggtaat tactattatt attgatattt aagttaaacc cagtaaatga     3600

agtccatgga ataatagaaa gagaaaaagc attttcaggt ataggtgttt tgggaaacaa     3660

tttccccgaa ccattatatt tctctacatc agaaaggtat aaatcataaa actctttgaa     3720

gtcattcttt acaggagtcc aaataccaga gaatgtttta gatacaccat caaaaattgt     3780

ataaagtggc tctaacttat cccaataacc taactctccg tcgctattgt aaccagttct     3840

aaaagctgta tttgagttta tcacccttgt cactaagaaa ataaatgcag ggtaaaattt     3900

atatccttct tgttttatgt ttcggtataa aacactaata tcaatttctg tggttatact     3960

aaaagtcgtt tgttggttca aataatgatt aaatatctct tttctcttcc aattgtctaa     4020

atcaatttta ttaaagttca ttaatatcgc ctcctattgt aaattaaaat ttaatttaat     4080

gcctctatta gctgccataa agcatgaatg cagtaaaata ttcatcctct ggcaagtatc     4140

tcttgcacta tgattcattt tatattattt tattttcatt ttcaatagtt tagataaaaa     4200

ataattaatt ttttaacaaa gtatttttat cttcctttga tgtaattctt tgtttttttg     4260

tccattaata ttatggacaa tttttttatt tctagtcaat aattttaatc tttattaaaa     4320

cctggcaatg ccagagagat acagtaatta ttgcatataa aagaaaacag acgcgccctg     4380

tttgtttcct gaaaaaccac ctcgtcctcc aaagtatcca gctttttaaa atccatcctc     4440

attcccagac gaaaaagttt tgacaaagcc tcttttcttg tccatagaat tgtactctgc     4500

acacaatatt catcagtatt tccaaaactc tttagaatct tttcctctct ttcggaaaag     4560

aaatacttta tcaatgcatc ctcgggcaaa aaaatctttt ccatgtctat tcccaccgcc     4620

ctgtccgaaa ccattccgat acaatacgga aacgcatggg ttattgatac acccatgtca     4680

gaatactggc cggcctcgcg aggatccaag cttactagct atgggaaaca aaatattgcg     4740

tatgcgactg ttcacatgga cgagaaaacc cctcacatgc atttaggagt tgttcctatg     4800

cgcctagagg gcttttcgtg cgtcagcatg agcgatcgga agaaaagaag aatacttttt     4860

cgcttcaaga tgttttgcag cgtgatcgag aacttcgtga gcaaagaaaa gcaaagagga     4920

aaaaatcgca tgatttggag cgataagaaa aagcactcga atgagtgctt tttttgcgtt     4980

ttgagcgtag cgaaaaacga gttctttcta ttcttgatac atatagaaat aacgtcattt     5040

ttattttagt tgctgaaagg tgcgttgaag tgttggtatg tatgtgtttt aaagtattga     5100

aaacccttaa aattggttgc acagaaaaac cccatctgtt aaagttataa gtgaccaaac     5160

aaataactaa atagatgggg gtttctttta atattatgtg tcctaatagt agcatttatt     5220

cagatgaaaa atcaagggtt ttagtggaca agacaaaaag tggaaaagtg agaccatgga     5280

gagaaaagaa aatcgctaat gttgattact ttgaacttct gcatattctt gaatttaaaa     5340

aggctgaaag agtaaaagat tgtgctgaaa tattagagta taaacaaaat cgtgaaacag     5400

gcgaaagaaa gttgtatcga gtgtggtttt gtaaatccag gctttgtcca atgtgcaact     5460

ggaggagagc aatgaaacat ggcattcagt cacaaaaggt tgttgctgaa gttattaaac     5520

aaaagccaac agttcgttgg ttgtttctca cattaacagt taaaaatgtt tatgatggcg     5580

aagaattaaa taagagtttg tcagatatgg ctcaaggatt tcgccgaatg atgcaatata     5640

aaaaaattaa taaaaatctt gttggtttta tgcgtgcaac ggaagtgaca ataaataata     5700

aagataattc ttataatcag cacatgcatg tattggtatg tgtggaacca acttatttta     5760

agaatacaga aaactacgtg aatcaaaaac aatggattca attttggaaa aaggcaatga     5820

aattagacta tgatccaaat gtaaaagttc aaatgattcg accgaaaaat aaatataaat     5880

cggatataca atcggcaatt gacgaaactg caaaatatcc tgtaaaggat acggatttta     5940

tgaccgatga tgaagaaaag aatttgaaac gtttgtctga tttggaggaa ggtttacacc     6000

gtaaaaggtt aatctcctat ggtggtttgt taaaagaaat acataaaaaa ttaaaccttg     6060

atgacacaga agaaggcgat ttgattcata cagatgatga cgaaaaagcc gatgaagatg     6120

gattttctat tattgcaatg tggaattggg aacggaaaaa ttattttatt aaagagtagt     6180

tcaacaaacg ggattgactt ttaaaaaagg attgattcta atgaagaaag cagacaagta     6240

agcctcctaa attcactag                                                  6259


<210>  53
<211>  67
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  53
acaagaaacc tttgtatatt ttttagtcca tatcttctca gaattctttc tcctccttct       60

tttatcc                                                                 67


<210>  54
<211>  72
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  54
aaaaaccggc atattggtgt taagtgaaag acgacggcag ggaaatatta aaatggaaat       60

gttgaaaaaa tg                                                           72


<210>  55
<211>  55
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  55
caagatcaca gaaaggggtt gtctattgtt catctccatt ctcccttcat atagc            55


<210>  56
<211>  29
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  56
gagcgatgac aagggagtaa ttttagatc                                         29


<210>  57
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  57
ttcgactatt tcccttagct cctctttctc                                        30


<210>  58
<211>  45
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  58
tttaaggagg acgaaagatg aaagttacaa ttataggagc ttctg                       45


<210>  59
<211>  45
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  59
ttaagggatt ttggtttata agtttttaac ttcttcacag tattt                       45


<210>  60
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  60
ctttcgtcct ccttaaaatt ttcg                                              24


<210>  61
<211>  44
<212>  DNA
<213>  Artificial

<220>
<223>  primer

<400>  61
aagcctccta aattcactag gagtcgtgac taagaacgtc aaag                        44


