                         SEQUENCE LISTING

<110>  KIM, HWAN
       MISSIAKAS, DOMINIQUE M.
       SCHNEEWIND, OLAF
 
<120>  COMPOSITIONS AND METHODS RELATED TO STAPHYLOCOCCAL SBI

<130>  ARCD.P0531WO

<140>  UNKNOWN
<141>  2013-03-15

<150>  61/618,465
<151>  2012-03-30

<150>  61/615,083
<151>  2012-03-23

<160>  20    

<170>  PatentIn version 3.5

<210>  1
<211>  520
<212>  PRT
<213>  Staphylococcus aureus

<400>  1

Met Leu Thr Leu Gln Ile His Thr Gly Gly Ile Asn Leu Lys Lys Lys 
1               5                   10                  15      


Asn Ile Tyr Ser Ile Arg Lys Leu Gly Val Gly Ile Ala Ser Val Thr 
            20                  25                  30          


Leu Gly Thr Leu Leu Ile Ser Gly Gly Val Thr Pro Ala Ala Asn Ala 
        35                  40                  45              


Ala Gln His Asp Glu Ala Gln Gln Asn Ala Phe Tyr Gln Val Leu Asn 
    50                  55                  60                  


Met Pro Asn Leu Asn Ala Asp Gln Arg Asn Gly Phe Ile Gln Ser Leu 
65                  70                  75                  80  


Lys Asp Asp Pro Ser Gln Ser Ala Asn Val Leu Gly Glu Ala Gln Lys 
                85                  90                  95      


Leu Asn Asp Ser Gln Ala Pro Lys Ala Asp Ala Gln Gln Asn Asn Phe 
            100                 105                 110         


Asn Lys Asp Gln Gln Ser Ala Phe Tyr Glu Ile Leu Asn Met Pro Asn 
        115                 120                 125             


Leu Asn Glu Ala Gln Arg Asn Gly Phe Ile Gln Ser Leu Lys Asp Asp 
    130                 135                 140                 


Pro Ser Gln Ser Thr Asn Val Leu Gly Glu Ala Lys Lys Leu Asn Glu 
145                 150                 155                 160 


Ser Gln Ala Pro Lys Ala Asp Asn Asn Phe Asn Lys Glu Gln Gln Asn 
                165                 170                 175     


Ala Phe Tyr Glu Ile Leu Asn Met Pro Asn Leu Asn Glu Glu Gln Arg 
            180                 185                 190         


Asn Gly Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn 
        195                 200                 205             


Leu Leu Ser Glu Ala Lys Lys Leu Asn Glu Ser Gln Ala Pro Lys Ala 
    210                 215                 220                 


Asp Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile Leu 
225                 230                 235                 240 


His Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn Gly Phe Ile Gln Ser 
                245                 250                 255     


Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala Lys 
            260                 265                 270         


Lys Leu Asn Asp Ala Gln Ala Pro Lys Ala Asp Asn Lys Phe Asn Lys 
        275                 280                 285             


Glu Gln Gln Asn Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Thr 
    290                 295                 300                 


Glu Glu Gln Arg Asn Gly Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser 
305                 310                 315                 320 


Val Ser Lys Glu Ile Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln 
                325                 330                 335     


Ala Pro Lys Glu Glu Asp Asn Asn Lys Pro Gly Lys Glu Asp Gly Asn 
            340                 345                 350         


Lys Pro Gly Lys Glu Asp Asn Asn Lys Pro Gly Lys Glu Asp Asn Lys 
        355                 360                 365             


Lys Pro Gly Lys Glu Asp Asn Asn Lys Pro Gly Lys Glu Asp Asn Asn 
    370                 375                 380                 


Lys Pro Gly Lys Glu Asp Gly Asn Lys Pro Gly Lys Glu Asp Asn Lys 
385                 390                 395                 400 


Lys Pro Gly Lys Glu Asp Asn Asn Lys Pro Gly Lys Glu Asp Gly Asn 
                405                 410                 415     


Lys Pro Gly Lys Glu Asp Gly Asn Gly Val His Val Val Lys Pro Gly 
            420                 425                 430         


Asp Thr Val Asn Asp Ile Ala Lys Ala Asn Gly Thr Thr Ala Asp Lys 
        435                 440                 445             


Ile Ala Ala Asp Asn Lys Leu Ala Asp Lys Asn Met Ile Lys Pro Gly 
    450                 455                 460                 


Gln Glu Leu Val Val Asp Lys Lys Gln Pro Ala Asn His Ala Asp Ala 
465                 470                 475                 480 


Asn Lys Ala Gln Ala Leu Pro Glu Thr Gly Glu Glu Asn Pro Phe Ile 
                485                 490                 495     


Gly Thr Thr Val Phe Gly Gly Leu Ser Leu Ala Leu Gly Ala Ala Leu 
            500                 505                 510         


Leu Ala Gly Arg Arg Arg Glu Leu 
        515                 520 


<210>  2
<211>  54
<212>  PRT
<213>  Staphylococcus sp.

<400>  2

Gln Gln Asn Asn Phe Asn Lys Asp Gln Gln Ser Ala Phe Tyr Glu Ile 
1               5                   10                  15      


Leu Asn Met Pro Asn Leu Asn Glu Ala Gln Arg Asn Gly Phe Ile Gln 
            20                  25                  30          


Ser Leu Lys Asp Asp Pro Ser Gln Ser Thr Asn Val Leu Gly Glu Ala 
        35                  40                  45              


Lys Lys Leu Asn Glu Ser 
    50                  


<210>  3
<211>  51
<212>  PRT
<213>  Staphylococcus sp.

<400>  3

Gln His Asp Glu Ala Gln Gln Asn Ala Phe Tyr Gln Val Leu Asn Met 
1               5                   10                  15      


Pro Asn Leu Asn Ala Asp Gln Arg Asn Gly Phe Ile Gln Ser Leu Lys 
            20                  25                  30          


Asp Asp Pro Ser Gln Ser Ala Asn Val Leu Gly Glu Ala Gln Lys Leu 
        35                  40                  45              


Asn Asp Ser 
    50      


<210>  4
<211>  52
<212>  PRT
<213>  Staphylococcus sp.

<400>  4

Asn Asn Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile Leu Asn 
1               5                   10                  15      


Met Pro Asn Leu Asn Glu Glu Gln Arg Asn Gly Phe Ile Gln Ser Leu 
            20                  25                  30          


Lys Asp Asp Pro Ser Gln Ser Ala Asn Leu Leu Ser Glu Ala Lys Lys 
        35                  40                  45              


Leu Asn Glu Ser 
    50          


<210>  5
<211>  52
<212>  PRT
<213>  Staphylococcus sp.

<400>  5

Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile Leu His 
1               5                   10                  15      


Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn Gly Phe Ile Gln Ser Leu 
            20                  25                  30          


Lys Asp Asp Pro Ser Val Ser Lys Glu Ile Leu Ala Glu Ala Lys Lys 
        35                  40                  45              


Leu Asn Asp Ala 
    50          


<210>  6
<211>  52
<212>  PRT
<213>  Staphylococcus sp.

<400>  6

Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile Leu His 
1               5                   10                  15      


Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn Gly Phe Ile Gln Ser Leu 
            20                  25                  30          


Lys Asp Asp Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala Lys Lys 
        35                  40                  45              


Leu Asn Asp Ala 
    50          


<210>  7
<211>  291
<212>  PRT
<213>  Staphylococcus aureus

<400>  7

Ala Gln His Asp Glu Ala Lys Lys Asn Ala Phe Tyr Gln Val Leu Asn 
1               5                   10                  15      


Met Pro Asn Leu Asn Ala Asp Gln Arg Asn Gly Phe Ile Gln Ser Leu 
            20                  25                  30          


Lys Ala Ala Pro Ser Gln Ser Ala Asn Val Leu Gly Glu Ala Gln Lys 
        35                  40                  45              


Leu Asn Asp Ser Gln Ala Pro Lys Ala Asp Ala Gln Gln Asn Asn Phe 
    50                  55                  60                  


Asn Lys Asp Lys Lys Ser Ala Phe Tyr Glu Ile Leu Asn Met Pro Asn 
65                  70                  75                  80  


Leu Asn Glu Ala Gln Arg Asn Gly Phe Ile Gln Ser Leu Lys Ala Ala 
                85                  90                  95      


Pro Ser Gln Ser Thr Asn Val Leu Gly Glu Ala Lys Lys Leu Asn Glu 
            100                 105                 110         


Ser Gln Ala Pro Lys Ala Asp Asn Asn Phe Asn Lys Glu Lys Lys Asn 
        115                 120                 125             


Ala Phe Tyr Glu Ile Leu Asn Met Pro Asn Leu Asn Glu Glu Gln Arg 
    130                 135                 140                 


Asn Gly Phe Ile Gln Ser Leu Lys Ala Ala Pro Ser Gln Ser Ala Asn 
145                 150                 155                 160 


Leu Leu Ser Glu Ala Lys Lys Leu Asn Glu Ser Gln Ala Pro Lys Ala 
                165                 170                 175     


Asp Asn Lys Phe Asn Lys Glu Lys Lys Asn Ala Phe Tyr Glu Ile Leu 
            180                 185                 190         


His Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn Gly Phe Ile Gln Ser 
        195                 200                 205             


Leu Lys Ala Ala Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala Lys 
    210                 215                 220                 


Lys Leu Asn Asp Ala Gln Ala Pro Lys Ala Asp Asn Lys Phe Asn Lys 
225                 230                 235                 240 


Glu Lys Lys Asn Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Thr 
                245                 250                 255     


Glu Glu Gln Arg Asn Gly Phe Ile Gln Ser Leu Lys Ala Ala Pro Ser 
            260                 265                 270         


Val Ser Lys Glu Ile Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln 
        275                 280                 285             


Ala Pro Lys 
    290     


<210>  8
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  8
aaaaaagcta gctggtctca tcctcaattt gagaagacgc aacaaacttc aactaag          57


<210>  9
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  9
aaaaaactcg agtttccaga atgataataa attac                                  35


<210>  10
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide

<400>  10

Trp Ser His Pro Gln Phe Glu Lys 
1               5               


<210>  11
<211>  436
<212>  PRT
<213>  Staphylococcus aureus

<400>  11

Met Lys Asn Lys Tyr Ile Ser Lys Leu Leu Val Gly Ala Ala Thr Ile 
1               5                   10                  15      


Thr Leu Ala Thr Met Ile Ser Asn Gly Glu Ala Lys Ala Ser Glu Asn 
            20                  25                  30          


Thr Gln Gln Thr Ser Thr Lys His Gln Thr Thr Gln Asn Asn Tyr Val 
        35                  40                  45              


Thr Asp Gln Gln Lys Ala Phe Tyr Gln Val Leu His Leu Lys Gly Ile 
    50                  55                  60                  


Thr Glu Glu Gln Arg Asn Gln Tyr Ile Lys Thr Leu Arg Glu His Pro 
65                  70                  75                  80  


Glu Arg Ala Gln Glu Val Phe Ser Glu Ser Leu Lys Asp Ser Lys Asn 
                85                  90                  95      


Pro Asp Arg Arg Val Ala Gln Gln Asn Ala Phe Tyr Asn Val Leu Lys 
            100                 105                 110         


Asn Asp Asn Leu Thr Glu Gln Glu Lys Asn Asn Tyr Ile Ala Gln Ile 
        115                 120                 125             


Lys Glu Asn Pro Asp Arg Ser Gln Gln Val Trp Val Glu Ser Val Gln 
    130                 135                 140                 


Ser Ser Lys Ala Lys Glu Arg Gln Asn Ile Glu Asn Ala Asp Lys Ala 
145                 150                 155                 160 


Ile Lys Asp Phe Gln Asp Asn Lys Ala Pro His Asp Lys Ser Ala Ala 
                165                 170                 175     


Tyr Glu Ala Asn Ser Lys Leu Pro Lys Asp Leu Arg Asp Lys Asn Asn 
            180                 185                 190         


Arg Phe Val Glu Lys Val Ser Ile Glu Lys Ala Ile Val Arg His Asp 
        195                 200                 205             


Glu Arg Val Lys Ser Ala Asn Asp Ala Ile Ser Lys Leu Asn Glu Lys 
    210                 215                 220                 


Asp Ser Ile Glu Asn Arg Arg Leu Ala Gln Arg Glu Val Asn Lys Ala 
225                 230                 235                 240 


Pro Met Asp Val Lys Glu His Leu Gln Lys Gln Leu Asp Ala Leu Val 
                245                 250                 255     


Ala Gln Lys Asp Ala Glu Lys Lys Val Ala Pro Lys Val Glu Ala Pro 
            260                 265                 270         


Gln Ile Gln Ser Pro Gln Ile Glu Lys Pro Lys Val Glu Ser Pro Lys 
        275                 280                 285             


Val Glu Val Pro Gln Ile Gln Ser Pro Lys Val Glu Val Pro Gln Ser 
    290                 295                 300                 


Lys Leu Leu Gly Tyr Tyr Gln Ser Leu Lys Asp Ser Phe Asn Tyr Gly 
305                 310                 315                 320 


Tyr Lys Tyr Leu Thr Asp Thr Tyr Lys Ser Tyr Lys Glu Lys Tyr Asp 
                325                 330                 335     


Thr Ala Lys Tyr Tyr Tyr Asn Thr Tyr Tyr Lys Tyr Lys Gly Ala Ile 
            340                 345                 350         


Asp Gln Thr Val Leu Thr Val Leu Gly Ser Gly Ser Lys Ser Tyr Ile 
        355                 360                 365             


Gln Pro Leu Lys Val Asp Asp Lys Asn Gly Tyr Leu Ala Lys Ser Tyr 
    370                 375                 380                 


Ala Gln Val Arg Asn Tyr Val Thr Glu Ser Ile Asn Thr Gly Lys Val 
385                 390                 395                 400 


Leu Tyr Thr Phe Tyr Gln Asn Pro Thr Leu Val Lys Thr Ala Ile Lys 
                405                 410                 415     


Ala Gln Glu Thr Ala Ser Ser Ile Lys Asn Thr Leu Ser Asn Leu Leu 
            420                 425                 430         


Ser Phe Trp Lys 
        435     


<210>  12
<211>  223
<212>  PRT
<213>  Staphylococcus aureus

<400>  12

Thr Gln Gln Thr Ser Thr Lys His Gln Thr Thr Gln Asn Asn Tyr Val 
1               5                   10                  15      


Thr Asp Gln Gln Lys Ala Phe Tyr Gln Val Leu His Leu Lys Gly Ile 
            20                  25                  30          


Thr Glu Glu Gln Arg Asn Gln Tyr Ile Lys Thr Leu Arg Glu His Pro 
        35                  40                  45              


Glu Arg Ala Gln Glu Val Phe Ser Glu Ser Leu Lys Asp Ser Lys Asn 
    50                  55                  60                  


Pro Asp Arg Arg Val Ala Gln Gln Asn Ala Phe Tyr Asn Val Leu Lys 
65                  70                  75                  80  


Asn Asp Asn Leu Thr Glu Gln Glu Lys Asn Asn Tyr Ile Ala Gln Ile 
                85                  90                  95      


Lys Glu Asn Pro Asp Arg Ser Gln Gln Val Trp Val Glu Ser Val Gln 
            100                 105                 110         


Ser Ser Lys Ala Lys Glu Arg Gln Asn Ile Glu Asn Ala Asp Lys Ala 
        115                 120                 125             


Ile Lys Asp Phe Gln Asp Asn Lys Ala Pro His Asp Lys Ser Ala Ala 
    130                 135                 140                 


Tyr Glu Ala Asn Ser Lys Leu Pro Lys Asp Leu Arg Asp Lys Asn Asn 
145                 150                 155                 160 


Arg Phe Val Glu Lys Val Ser Ile Glu Lys Ala Ile Val Arg His Asp 
                165                 170                 175     


Glu Arg Val Lys Ser Ala Asn Asp Ala Ile Ser Lys Leu Asn Glu Lys 
            180                 185                 190         


Asp Ser Ile Glu Asn Arg Arg Leu Ala Gln Arg Glu Val Asn Lys Ala 
        195                 200                 205             


Pro Met Asp Val Lys Glu His Leu Gln Lys Gln Leu Asp Ala Leu 
    210                 215                 220             


<210>  13
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant polypeptide Sbi1-4KKAA

<400>  13

Thr Gln Gln Thr Ser Thr Lys His Gln Thr Thr Gln Asn Asn Tyr Val 
1               5                   10                  15      


Thr Asp Lys Lys Lys Ala Phe Tyr Gln Val Leu His Leu Lys Gly Ile 
            20                  25                  30          


Thr Glu Glu Gln Arg Asn Gln Tyr Ile Lys Thr Leu Arg Glu His Pro 
        35                  40                  45              


Glu Arg Ala Gln Glu Val Phe Ser Glu Ser Leu Lys Asp Ser Lys Asn 
    50                  55                  60                  


Pro Asp Arg Arg Val Ala Lys Lys Asn Ala Phe Tyr Asn Val Leu Lys 
65                  70                  75                  80  


Asn Asp Asn Leu Thr Glu Gln Glu Lys Asn Asn Tyr Ile Ala Gln Ile 
                85                  90                  95      


Lys Glu Asn Pro Asp Arg Ser Gln Gln Val Trp Val Glu Ser Val Gln 
            100                 105                 110         


Ser Ser Lys Ala Lys Glu Arg Gln Asn Ile Glu Asn Ala Asp Lys Ala 
        115                 120                 125             


Ile Lys Asp Phe Gln Asp Asn Lys Ala Pro His Asp Lys Ser Ala Ala 
    130                 135                 140                 


Tyr Glu Ala Asn Ser Lys Leu Pro Lys Asp Leu Arg Asp Lys Asn Asn 
145                 150                 155                 160 


Arg Phe Val Glu Lys Val Ser Ile Glu Lys Ala Ile Val Arg His Asp 
                165                 170                 175     


Glu Arg Val Lys Ser Ala Asn Asp Ala Ile Ser Lys Leu Asn Glu Lys 
            180                 185                 190         


Asp Ser Ile Glu Asn Arg Ala Leu Ala Gln Arg Glu Val Ala Lys Ala 
        195                 200                 205             


Pro Met Asp Val Lys Glu His Leu Gln Lys Gln Leu Asp Ala Leu 
    210                 215                 220             


<210>  14
<211>  50
<212>  PRT
<213>  Staphylococcus aureus

<400>  14

His Asp Glu Ala Gln Gln Asn Ala Phe Tyr Gln Val Leu Asn Met Pro 
1               5                   10                  15      


Asn Leu Asn Ala Asp Gln Arg Asn Gly Phe Ile Gln Ser Leu Lys Asp 
            20                  25                  30          


Asp Pro Ser Gln Ser Ala Asn Val Leu Gly Glu Ala Gln Lys Leu Asn 
        35                  40                  45              


Asp Ser 
    50  


<210>  15
<211>  50
<212>  PRT
<213>  Staphylococcus aureus

<400>  15

Phe Asn Lys Asp Gln Gln Ser Ala Phe Tyr Glu Ile Leu Asn Met Pro 
1               5                   10                  15      


Asn Leu Asn Glu Ala Gln Arg Asn Gly Phe Ile Gln Ser Leu Lys Asp 
            20                  25                  30          


Asp Pro Ser Gln Ser Thr Asn Val Leu Gly Glu Ala Lys Lys Leu Asn 
        35                  40                  45              


Glu Ser 
    50  


<210>  16
<211>  50
<212>  PRT
<213>  Staphylococcus aureus

<400>  16

Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile Leu Asn Met Pro 
1               5                   10                  15      


Asn Leu Asn Glu Glu Gln Arg Asn Gly Phe Ile Gln Ser Leu Lys Asp 
            20                  25                  30          


Asp Pro Ser Gln Ser Ala Asn Leu Leu Ser Glu Ala Lys Lys Leu Asn 
        35                  40                  45              


Glu Ser 
    50  


<210>  17
<211>  50
<212>  PRT
<213>  Staphylococcus aureus

<400>  17

Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile Leu His Leu Pro 
1               5                   10                  15      


Asn Leu Asn Glu Glu Gln Arg Asn Gly Phe Ile Gln Ser Leu Lys Asp 
            20                  25                  30          


Asp Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala Lys Lys Leu Asn 
        35                  40                  45              


Asp Ala 
    50  


<210>  18
<211>  50
<212>  PRT
<213>  Staphylococcus aureus

<400>  18

Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile Leu His Leu Pro 
1               5                   10                  15      


Asn Leu Thr Glu Glu Gln Arg Asn Gly Phe Ile Gln Ser Leu Lys Asp 
            20                  25                  30          


Asp Pro Ser Val Ser Lys Glu Ile Leu Ala Glu Ala Lys Lys Leu Asn 
        35                  40                  45              


Asp Ala 
    50  


<210>  19
<211>  45
<212>  PRT
<213>  Staphylococcus aureus

<400>  19

Asp Gln Gln Lys Ala Phe Tyr Gln Val Leu His Leu Lys Gly Ile Thr 
1               5                   10                  15      


Glu Glu Gln Arg Asn Gln Tyr Ile Lys Thr Leu Arg Glu His Pro Glu 
            20                  25                  30          


Arg Ala Gln Glu Val Phe Ser Glu Ser Leu Lys Asp Ser 
        35                  40                  45  


<210>  20
<211>  45
<212>  PRT
<213>  Staphylococcus aureus

<400>  20

Ala Gln Gln Asn Ala Phe Tyr Asn Val Leu Lys Asn Asp Asn Leu Thr 
1               5                   10                  15      


Glu Gln Glu Lys Asn Asn Tyr Ile Ala Gln Ile Lys Glu Asn Pro Asp 
            20                  25                  30          


Arg Ser Gln Gln Val Trp Val Glu Ser Val Gln Ser Ser 
        35                  40                  45  


