                         SEQUENCE LISTING

<110>  BIOCON LIMITED
 
<120>  SECRETION OF FUNCTIONAL INSULIN GLARGINE DIRECTLY INTO THE 
       CULTURE MEDIUM THROUGH OVER EXPRESSION OF KEX2P INTRACELLULARLY

<130>  PCT1218/SFrk

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  159
<212>  DNA
<213>  Synthetic Sequence


<220>
<221>  gene
<222>  (1)..(159)

<400>  1
tttgttaacc aacatttgtg tggttctcat ttggttgaag ctttgtactt ggtttgtggt       60

gaaagaggtt ttttttacac tccaaagact agaagaggta ttgttgaaca atgttgtact      120

tctatttgtt ctttgtacca attggaaaac tactgtggt                             159


<210>  2
<211>  1983
<212>  DNA
<213>  Pichia pastoris


<220>
<221>  Sequence of truncated Kex2P
<222>  (1)..(1983)

<400>  2
atgtatttgc cagcacttcg cttagcatgc tggatcttaa ttggtcttag gtctacggag       60

gctttggaga cttccgagag agagatcttt gctctcaagc tggataaatc ctggcttcca      120

acgtttctag aaacgttcca agataagttc aggtatgaaa gacagatcaa cggtttggat      180

gactaccatg ttttttcaca cagtaagaac gaagagtttc agttagagaa ctttaaagtg      240

aagactcttt tgacgcgaga caacgccaat cttcactccg aactgatttc ccacaatgtg      300

gacgaggttc acatgctaag gccctctcat tatttgcata aacgagctcc tgttgtgatg      360

gacaagtcag aggaattaag agaacaaata gcgaaggatt ttgacattga tgacccttta      420

tttgctaaac agtggcatct atttaatcct cgttacccag gacacgacgt gaacgtgtcg      480

caagtttggt acgatggtat cactggaaaa ggtgtagtga ccgccatagt tgatgacgga      540

ctagatatgg acagtaaaga tctcaaagaa tctttttgtg aggaaggatc ttgggatttc      600

aatgccaaca ctagactacc caaaccaaga cttagagacg atcaccacgg aaccagatgt      660

gcagcggaga ttgcagctaa gaagggaaat aaatactgtg gagttggtgt ggcatatgat      720

tcaaaggttt ctggcatcag gattcttagt gataaaatca caccagagga tgaagctctc      780

tccttaatct acggtcttga tgtcaacgac atttattcat gttcatgggg gccagcagac      840

aatggaatca caatgcaagg tcccagctcg ttagtcaaag aagccatgct taaaggagtt      900

caggatggaa gaaagggtaa aggtgcgctg tatgtattcg ccagtggaaa cggagcatct      960

tctggtgata actgcaattt tgacgggtac accaatagca tttattccat aacagttggg     1020

gcaattgata ttaaagggct tcatccacca tacgctgagg cttgctctgc tgtgatgact     1080

gtcacataca gttctggatc gggtgagcac atacacacaa ccgacatcaa cgataaatgt     1140

tctgataccc atggaggaac atccgctgct gcacctttag cggctggtct ttattctttg     1200

gtttatcagg ctaatccgga cctgacttgg cgagatattc aatggctgac tgttttaaca     1260

gccgttcctg ttaacgaaca ggagcctggc tggcagaaga ctgctatcgg taagatgtat     1320

tctcataaat acggatatgg caagatcgat gcatatgcac tggtcaatct agcaagatct     1380

ccagacttcc cgtatctcaa accacaaagc tggatttatg gcactgaggt tcacgaaagc     1440

ttgaatactt ccgaagctaa cggtgtgctg acatccaagt atgaattgac ccaggaggcc     1500

aaagatctaa tgaactttga aaaaattgag catgttacgg ttactgtaga tataaaggcg     1560

gcggaaagag gtaaagttct tgttgagttg atctcccctt caggtgttgt cagtgaattg     1620

gctccctatc gaagaatgga caaggataag gaaggatttc caaattggac gttcatgtca     1680

gtagctcatt ggggtgaaga cgggttagga gagtggatat tgaaaatcac taacaaagaa     1740

ggaaattctg tggtgcttaa ctcctggcaa ataaaattct ttggagaaag tcaagaccct     1800

gaaaaggctg aaaaattctc tttaactaag aaatatgacg aaatattagt caaccctcca     1860

tcttcatcta cttccacgac agtggacacc tcatctacag aagccacttt ttcgtcttcc     1920

tctgtttcag aggcttcagc cacggaaacg gatgtaaaag agacttctac aaccggtgat     1980

taa                                                                   1983


<210>  3
<211>  603
<212>  DNA
<213>  Pichia pastoris


<220>
<221>  promoter
<222>  (1)..(603)

<400>  3
ggatccgcat gcaggaatct ctggcacggt gctaatggta gttatccaac ggagctgagg       60

tagtcgatat atctggatat gccgcctata ggataaaaac aggagagggt gaaccttgct      120

tatggctact agattgttct tgtactctga attctcatta tgggaaacta aactaatctc      180

atctgtgtgt tgcagtacta ttgaatcgtt gtagtatcta cctggagggc attccatgaa      240

ttagtgagat aacagagttg ggtaactaga gagaataata gacgtatgca tgattactac      300

acaacggatg tcgcactctt tccttagtta aaactatcat ccaatcacaa gatgcgggct      360

ggaaagactt gctcccgaag gataatcttc tgcttctatc tcccttcctc atatggtttc      420

gcagggctca tgccccttct tccttcgaac tgcccgatga ggaagtcctt agcctatcaa      480

agaattcggg accatcatcg atttttagag ccttacctga tcgcaatcag gatttcacta      540

ctcatataaa tacatcgctc aaagctccaa ctttgcttgt tcatacaatt cttgatattc      600

aca                                                                    603


<210>  4
<211>  483
<212>  DNA
<213>  Pichia pastoris


<220>
<221>  promoter
<222>  (1)..(483)

<400>  4
agatcttttt tgtagaaatg tcttggtgtc ctcgtccaat caggtagcca tctctgaaat       60

atctggctcc gttgcaactc cgaacgacct gctggcaacg taaaattctc cggggtaaaa      120

cttaaatgtg gagtaatgga accagaaacg tctcttccct tctctctcct tccaccgccc      180

gttaccgtcc ctaggaaatt ttactctgct ggagagcttc ttctacggcc cccttgcagc      240

aatgctcttc ccagcattac gttgcgggta aaacggaggt cgtgtacccg acctagcagc      300

ccagggatgg aaaagtcccg gccgtcgctg gcaataatag cgggcggacg catgtcatga      360

gattattgga aaccaccaga atcgaatata aaaggcgaac acctttccca attttggttt      420

ctcctgaccc aaagacttta aatttaattt atttgtccct atttcaatca attgaacaac      480

tat                                                                    483


<210>  5
<211>  31
<212>  DNA
<213>  Pichia pastoris


<220>
<221>  primer_bind
<222>  (1)..(31)

<400>  5
gcggatccgc atgcaggaat ctctggcacg g                                      31


<210>  6
<211>  36
<212>  DNA
<213>  Pichia pastoris


<220>
<221>  primer_bind
<222>  (1)..(36)

<400>  6
gcgaagtgct ggcaaataca ttgtgaatat caagaa                                 36


<210>  7
<211>  34
<212>  DNA
<213>  Pichia pastoris


<220>
<221>  primer_bind
<222>  (1)..(34)

<400>  7
caattcttga tattcacaat gtatttgcca gcac                                   34


<210>  8
<211>  31
<212>  DNA
<213>  Pichia pastoris


<220>
<221>  primer_bind
<222>  (1)..(31)

<400>  8
ggagctcgtt tatgcaaata atgagagggc c                                      31


