                         SEQUENCE LISTING

<110>  Nutrilab N.V.
 
<120>  Improved galactose isomerases and use thereof in the production 
       of tagatose

<130>  P6036885PCT

<150>  US 61/620,002
<151>  2012-04-04

<150>  EP 12163188.1
<151>  2012-04-04

<160>  8     

<170>  PatentIn version 3.3

<210>  1
<211>  496
<212>  PRT
<213>  Geobacilus stearothermophilus

<400>  1

Met Leu Ser Leu Arg Pro Tyr Glu Phe Trp Phe Val Thr Gly Ser Gln 
1               5                   10                  15      


His Leu Tyr Gly Glu Glu Ala Leu Lys Gln Val Glu Glu His Ser Met 
            20                  25                  30          


Met Ile Val Asn Glu Leu Asn Gln Asp Ser Val Phe Pro Phe Pro Leu 
        35                  40                  45              


Val Phe Lys Ser Val Val Thr Thr Pro Glu Glu Ile Arg Arg Val Cys 
    50                  55                  60                  


Leu Glu Ala Asn Ala Ser Glu Gln Cys Ala Gly Val Ile Thr Trp Met 
65                  70                  75                  80  


His Thr Phe Ser Pro Ala Lys Met Trp Ile Gly Gly Leu Leu Glu Leu 
                85                  90                  95      


Arg Lys Pro Leu Leu His Leu His Thr Gln Phe Asn Arg Asp Ile Pro 
            100                 105                 110         


Trp Asp Ser Ile Asp Met Asp Phe Met Asn Leu Asn Gln Ser Ala His 
        115                 120                 125             


Gly Asp Arg Glu Tyr Gly Phe Ile Gly Ala Arg Met Gly Val Ala Arg 
    130                 135                 140                 


Lys Val Val Val Gly His Trp Glu Asp Pro Glu Val Arg Glu Arg Leu 
145                 150                 155                 160 


Ala Lys Trp Met Arg Thr Ala Val Ala Phe Ala Glu Ser Arg Asn Leu 
                165                 170                 175     


Lys Val Ala Arg Phe Gly Asp Asn Met Arg Glu Val Ala Val Thr Glu 
            180                 185                 190         


Gly Asp Lys Val Gly Ala Gln Ile Gln Phe Gly Trp Ser Val Asn Gly 
        195                 200                 205             


Tyr Gly Ile Gly Asp Leu Val Gln Tyr Ile Arg Asp Val Ser Glu Gln 
    210                 215                 220                 


Lys Val Asn Glu Leu Leu Asp Glu Tyr Glu Glu Leu Tyr Asp Ile Val 
225                 230                 235                 240 


Pro Ala Gly Arg Gln Glu Gly Pro Val Arg Glu Ser Ile Arg Glu Gln 
                245                 250                 255     


Ala Arg Ile Glu Leu Gly Leu Lys Ala Phe Leu Gln Asp Gly Asn Phe 
            260                 265                 270         


Thr Ala Phe Thr Thr Thr Phe Glu Asp Leu His Gly Met Lys Gln Leu 
        275                 280                 285             


Pro Gly Leu Ala Val Gln Arg Leu Met Ala Glu Gly Tyr Gly Phe Gly 
    290                 295                 300                 


Gly Glu Gly Asp Trp Lys Thr Ala Ala Leu Val Arg Leu Met Lys Val 
305                 310                 315                 320 


Met Ala Asp Gly Lys Gly Thr Ser Phe Met Glu Asp Tyr Thr Tyr His 
                325                 330                 335     


Leu Glu Pro Gly Asn Glu Met Ile Leu Gly Ala His Met Leu Glu Val 
            340                 345                 350         


Cys Pro Thr Ile Ala Ala Thr Arg Pro Arg Ile Glu Val His Pro Leu 
        355                 360                 365             


Ser Ile Gly Gly Lys Glu Asp Pro Ala Arg Leu Val Phe Asp Gly Gly 
    370                 375                 380                 


Glu Gly Ala Ala Val Asn Ala Ser Leu Ile Asp Leu Gly His Arg Phe 
385                 390                 395                 400 


Arg Leu Ile Val Asn Glu Val Asp Ala Val Lys Pro Glu His Asp Met 
                405                 410                 415     


Pro Lys Leu Pro Val Ala Arg Ile Leu Trp Lys Pro Arg Pro Ser Leu 
            420                 425                 430         


Arg Asp Ser Ala Glu Ala Trp Ile Leu Ala Gly Gly Ala His His Thr 
        435                 440                 445             


Cys Phe Ser Phe Ala Val Thr Thr Glu Gln Leu Gln Asp Phe Ala Glu 
    450                 455                 460                 


Met Ala Gly Ile Glu Cys Val Val Ile Asn Glu His Thr Ser Val Ser 
465                 470                 475                 480 


Ser Phe Lys Asn Glu Leu Lys Trp Asn Glu Val Phe Trp Arg Gly Arg 
                485                 490                 495     


<210>  2
<211>  496
<212>  PRT
<213>  Artificial

<220>
<223>  mutated L-arabinose isomerase from G. stearothermophilus

<400>  2

Met Leu Ser Leu Arg Pro Tyr Glu Phe Trp Phe Val Thr Gly Ser Gln 
1               5                   10                  15      


His Leu Tyr Gly Glu Glu Ala Leu Lys Gln Val Glu Glu His Ser Met 
            20                  25                  30          


Met Ile Val Asn Glu Leu Asn Gln Asp Ser Val Phe Pro Phe Pro Leu 
        35                  40                  45              


Val Phe Lys Ser Val Val Thr Thr Pro Glu Glu Ile Arg Arg Val Cys 
    50                  55                  60                  


Leu Glu Ala Asn Ala Ser Glu Gln Cys Ala Gly Val Ile Thr Trp Met 
65                  70                  75                  80  


His Thr Phe Ser Pro Ala Lys Met Trp Ile Gly Gly Leu Leu Glu Leu 
                85                  90                  95      


Arg Lys Pro Leu Leu His Leu His Thr Gln Phe Asn Arg Asp Ile Pro 
            100                 105                 110         


Trp Asp Ser Ile Asp Met Asp Phe Met Asn Leu Asn Gln Ser Ala His 
        115                 120                 125             


Gly Asp Arg Glu Tyr Gly Phe Ile Gly Ala Arg Met Gly Val Ala Arg 
    130                 135                 140                 


Lys Val Val Val Gly His Trp Glu Asp Pro Glu Val Arg Glu Arg Leu 
145                 150                 155                 160 


Ala Lys Trp Met Arg Thr Ala Val Ala Phe Ala Glu Ser Arg Asn Leu 
                165                 170                 175     


Lys Val Ala Arg Phe Gly Asp Asn Met Arg Glu Val Ala Val Thr Glu 
            180                 185                 190         


Gly Asp Lys Val Gly Ala Gln Ile Gln Phe Gly Trp Ser Val Asn Gly 
        195                 200                 205             


Tyr Gly Ile Gly Asp Leu Val Gln Tyr Ile Arg Asp Val Ser Glu Gln 
    210                 215                 220                 


Lys Val Asn Glu Leu Leu Asp Glu Tyr Glu Glu Leu Tyr Asp Ile Val 
225                 230                 235                 240 


Pro Ala Gly Arg Gln Glu Gly Pro Val Arg Glu Ser Ile Arg Glu Gln 
                245                 250                 255     


Ala Arg Ile Glu Leu Gly Leu Lys Ala Phe Leu Gln Asp Gly Asn Phe 
            260                 265                 270         


Thr Ala Phe Thr Thr Thr Phe Glu Asp Leu His Gly Met Lys Gln Leu 
        275                 280                 285             


Pro Gly Leu Ala Val Gln Arg Leu Met Ala Glu Gly Tyr Gly Phe Gly 
    290                 295                 300                 


Gly Glu Gly Asp Trp Lys Thr Ala Ala Leu Val Arg Leu Met Lys Val 
305                 310                 315                 320 


Met Ala Asp Gly Lys Gly Thr Ser Phe Met Glu Asp Tyr Thr Tyr His 
                325                 330                 335     


Leu Glu Pro Gly Asn Glu Leu Ile Leu Gly Ala His Met Leu Glu Val 
            340                 345                 350         


Asp Pro Thr Ile Ala Ala Thr Arg Pro Arg Ile Glu Val His Pro Leu 
        355                 360                 365             


Ser Ile Gly Gly Lys Glu Asp Pro Val Arg Leu Val Phe Asp Gly Gly 
    370                 375                 380                 


Glu Gly Ala Ala Val Asn Ala Ser Leu Ile Asp Leu Gly His Arg Phe 
385                 390                 395                 400 


Arg Leu Ile Val Asn Glu Val Asp Ala Val Lys Pro Glu His Asp Leu 
                405                 410                 415     


Pro Lys Leu Pro Val Ala Arg Ile Leu Trp Lys Pro Arg Pro Ser Phe 
            420                 425                 430         


Arg Asp Ser Ala Glu Ala Trp Ile Leu Ala Gly Gly Ala His His Thr 
        435                 440                 445             


Val Phe Ser Phe Ala Val Thr Thr Glu Gln Leu Gln Asp Phe Ala Glu 
    450                 455                 460                 


Ile Ala Gly Ile Glu Leu Val Val Ile Asn Glu His Thr Ser Val Ser 
465                 470                 475                 480 


Ser Phe Lys Asn Glu Leu Lys Trp Asn Glu Val Phe Trp Arg Gly Arg 
                485                 490                 495     


<210>  3
<211>  495
<212>  PRT
<213>  Artificial

<220>
<223>  mutated L-arabinose isomerase from G. stearothermophilus

<400>  3

Met Ser Leu Arg Pro Tyr Glu Phe Trp Phe Val Thr Gly Ser Gln His 
1               5                   10                  15      


Leu Tyr Gly Glu Glu Ala Leu Lys Gln Val Glu Glu His Ser Ser Lys 
            20                  25                  30          


Ile Val Asn Glu Leu Asn Gln Asp Ser Val Phe Pro Phe Pro Leu Val 
        35                  40                  45              


Phe Lys Ser Val Val Thr Thr Pro Glu Glu Ile Arg Arg Val Phe Leu 
    50                  55                  60                  


Glu Ala Asn Ala Ser Glu Gln Cys Ala Gly Val Ile Thr Trp Leu His 
65                  70                  75                  80  


Thr Phe Ser Pro Ala Lys Ser Trp Ile Gly Gly Leu Leu Glu Leu Arg 
                85                  90                  95      


Lys Pro Leu Leu His Leu His Thr Gln Phe Asn Arg Asp Ile Pro Trp 
            100                 105                 110         


Asp Ser Ile Asp Met Asp Phe Met Asn Leu Asn Gln Ser Ala His Gly 
        115                 120                 125             


Asp Arg Glu Tyr Gly Phe Ile Gly Ala Arg Leu Gly Val Ala Arg Lys 
    130                 135                 140                 


Val Val Val Gly His Trp Glu Asp Pro Glu Val Arg Glu Arg Leu Ala 
145                 150                 155                 160 


Lys Trp Met Arg Thr Ala Val Ala Phe Ala Glu Ser Arg Asn Leu Lys 
                165                 170                 175     


Val Ala Arg Phe Gly Asp Asn Met Arg Glu Val Ala Val Thr Glu Gly 
            180                 185                 190         


Asp Lys Val Gly Ala Gln Ile Gln Phe Gly Trp Ser Val Asn Thr Tyr 
        195                 200                 205             


Gly Ile Gly Asp Leu Val Gln Tyr Ile Arg Asp Val Ser Glu Gln Lys 
    210                 215                 220                 


Val Asn Glu Leu Leu Asp Glu Tyr Glu Glu Leu Tyr Asp Ile Val Pro 
225                 230                 235                 240 


Ala Gly Arg Gln Glu Gly Pro Val Arg Glu Ser Ile Arg Glu Gln Ala 
                245                 250                 255     


Arg Ile Glu Leu Gly Leu Lys Ala Phe Leu Gln Asp Gly Asn Phe Thr 
            260                 265                 270         


Ala Phe Thr Thr Thr Phe Glu Asp Leu His Gly Leu Lys Gln Leu Pro 
        275                 280                 285             


Gly Leu Ala Val Gln Arg Leu Leu Ala Glu Gly Tyr Gly Phe Gly Gly 
    290                 295                 300                 


Glu Gly Asp Trp Lys Thr Ala Ala Leu Val Arg Leu Leu Lys Val Leu 
305                 310                 315                 320 


Ala Asp Gly Lys Gly Thr Ser Phe Met Glu Asp Tyr Thr Tyr His Leu 
                325                 330                 335     


Glu Pro Gly Asn Glu Met Ile Leu Gly Ala His Met Leu Glu Val Cys 
            340                 345                 350         


Pro Thr Ile Ala Ala Thr Arg Pro Arg Ile Glu Val His Pro Leu Ser 
        355                 360                 365             


Ile Gly Gly Lys Glu Asp Pro Ala Arg Leu Val Phe Asp Gly Gly Glu 
    370                 375                 380                 


Gly Ala Ala Val Asn Ala Ser Leu Ile Asp Leu Gly His Arg Phe Arg 
385                 390                 395                 400 


Leu Ile Val Asn Glu Val Asp Ala Val Lys Pro Glu His Asp Met Pro 
                405                 410                 415     


Lys Leu Pro Val Ala Arg Ile Leu Trp Lys Pro Arg Pro Ser Leu Arg 
            420                 425                 430         


Asp Ser Ala Glu Ala Trp Ile Leu Ala Gly Gly Ala His His Thr Cys 
        435                 440                 445             


Phe Ser Phe Ala Val Thr Thr Glu Gln Leu Gln Asp Phe Ala Glu Met 
    450                 455                 460                 


Ala Gly Ile Glu Cys Val Val Ile Asn Glu His Thr Ser Val Ser Ser 
465                 470                 475                 480 


Phe Lys Asn Glu Leu Lys Trp Asn Glu Val Phe Trp Arg Gly Arg 
                485                 490                 495 


<210>  4
<211>  495
<212>  PRT
<213>  Artificial

<220>
<223>  mutated L-arabinose isomerase from G. stearothermophilus

<400>  4

Met Ser Leu Arg Pro Tyr Glu Phe Trp Phe Val Thr Gly Ser Gln His 
1               5                   10                  15      


Leu Tyr Gly Glu Glu Ala Leu Lys Gln Val Glu Glu His Ser Ser Lys 
            20                  25                  30          


Ile Val Asn Glu Leu Asn Gln Asp Ser Val Phe Pro Phe Pro Leu Val 
        35                  40                  45              


Phe Lys Ser Val Val Thr Thr Pro Glu Glu Ile Arg Arg Val Phe Leu 
    50                  55                  60                  


Glu Ala Asn Ala Ser Glu Gln Cys Ala Gly Val Ile Thr Trp Leu His 
65                  70                  75                  80  


Thr Phe Ser Pro Ala Lys Ser Trp Ile Gly Gly Leu Leu Glu Leu Arg 
                85                  90                  95      


Lys Pro Leu Leu His Leu His Thr Gln Phe Asn Arg Asp Ile Pro Trp 
            100                 105                 110         


Asp Ser Ile Asp Met Asp Phe Met Asn Leu Asn Gln Ser Ala His Gly 
        115                 120                 125             


Asp Arg Glu Tyr Gly Phe Ile Gly Ala Arg Leu Gly Val Ala Arg Lys 
    130                 135                 140                 


Val Val Val Gly His Trp Glu Asp Pro Glu Val Arg Glu Arg Leu Ala 
145                 150                 155                 160 


Lys Trp Met Arg Thr Ala Val Ala Phe Ala Glu Ser Arg Asn Leu Lys 
                165                 170                 175     


Val Ala Arg Phe Gly Asp Asn Met Arg Glu Val Ala Val Thr Glu Gly 
            180                 185                 190         


Asp Lys Val Gly Ala Gln Ile Gln Phe Gly Trp Ser Val Asn Thr Tyr 
        195                 200                 205             


Gly Ile Gly Asp Leu Val Gln Tyr Ile Arg Asp Val Ser Glu Gln Lys 
    210                 215                 220                 


Val Asn Glu Leu Leu Asp Glu Tyr Glu Glu Leu Tyr Asp Ile Val Pro 
225                 230                 235                 240 


Ala Gly Arg Gln Glu Gly Pro Val Arg Glu Ser Ile Arg Glu Gln Ala 
                245                 250                 255     


Arg Ile Glu Leu Gly Leu Lys Ala Phe Leu Gln Asp Gly Asn Phe Thr 
            260                 265                 270         


Ala Phe Thr Thr Thr Phe Glu Asp Leu His Gly Met Lys Gln Leu Pro 
        275                 280                 285             


Gly Leu Ala Val Gln Arg Leu Met Ala Glu Gly Tyr Gly Phe Gly Gly 
    290                 295                 300                 


Glu Gly Asp Trp Lys Thr Ala Ala Leu Val Arg Leu Met Lys Val Met 
305                 310                 315                 320 


Ala Asp Gly Lys Gly Thr Ser Phe Met Glu Asp Tyr Thr Tyr His Leu 
                325                 330                 335     


Glu Pro Gly Asn Glu Met Ile Leu Gly Ala His Met Leu Glu Val Cys 
            340                 345                 350         


Pro Thr Ile Ala Ala Thr Arg Pro Arg Ile Glu Val His Pro Leu Ser 
        355                 360                 365             


Ile Gly Gly Lys Glu Asp Pro Ala Arg Leu Val Phe Asp Gly Gly Glu 
    370                 375                 380                 


Gly Ala Ala Val Asn Ala Ser Leu Ile Asp Leu Gly His Arg Phe Arg 
385                 390                 395                 400 


Leu Ile Val Asn Glu Val Asp Ala Val Lys Pro Glu His Asp Met Pro 
                405                 410                 415     


Lys Leu Pro Val Ala Arg Ile Leu Trp Lys Pro Arg Pro Ser Leu Arg 
            420                 425                 430         


Asp Ser Ala Glu Ala Trp Ile Leu Ala Gly Gly Ala His His Thr Cys 
        435                 440                 445             


Phe Ser Phe Ala Val Thr Thr Glu Gln Leu Gln Asp Phe Ala Glu Met 
    450                 455                 460                 


Ala Gly Ile Glu Cys Val Val Ile Asn Glu His Thr Ser Val Ser Ser 
465                 470                 475                 480 


Phe Lys Asn Glu Leu Lys Trp Asn Glu Val Phe Trp Arg Gly Arg 
                485                 490                 495 


<210>  5
<211>  1491
<212>  DNA
<213>  Artificial

<220>
<223>  mutated L-arabinose isomerase from G. stearothermophilus

<400>  5
atgctgtcat tacgtcctta tgaattttgg tttgtaacgg gaagccagca cttgtacgga     60

gaagaagcat taaagcaagt tgaagagcat tcaatgatga ttgtcaatga gctgaatcaa    120

gattcagtgt tcccgttccc acttgttttc aaatcagttg tcacaacgcc agaggaaatc    180

cggcgcgttt gccttgaggc gaatgcgagc gaacaatgcg ctggggtcat cacttggatg    240

catacattct cgccagcgaa gatgtggatt ggcggccttt tggagctgcg aaaaccgtta    300

ttgcatcttc acactcaatt taaccgtgat attccgtggg acagcatcga tatggacttt    360

atgaacttaa accaatcggc tcacggtgac cgggaatacg gatttatcgg cgcgagaatg    420

ggcgtggccc ggaaagtggt ggtcgggcac tgggaagacc cagaagtccg cgagcggctg    480

gcgaaatgga tgcggacggc tgtcgcgttt gcggaaagcc gcaacctaaa agtggctcgt    540

tttggcgaca acatgcgtga agtggctgtg acggaagggg acaaagtcgg agcgcaaatt    600

caattcggct ggtcggtcaa cggctatggc atcggggatt tggtgcaata catccgcgat    660

gtttctgaac aaaaagtgaa cgagttgctc gatgaatacg aggagctgta cgacattgta    720

cccgccggcc gccaagaagg gcccgttcgc gaatcaattc gtgaacaggc gcggattgaa    780

ctcgggctga aagccttttt gcaggatggg aactttaccg ctttcacgac gacgttcgag    840

gacttgcatg ggatgaagca gctgccggga cttgccgttc agcgactcat ggcggaagga    900

tacggttttg gaggcgaggg agactggaaa acggctgcgc ttgtccggct aatgaaagtg    960

atggctgatg gaaaagggac gtcatttatg gaagattaca cgtaccacct tgagccgggt   1020

aatgaactga ttcttggcgc ccacatgttg gaagtagatc cgactattgc agcgacccgg   1080

ccgcgaatcg aagtccatcc gctttccatc ggtggaaaag aagatccagt gcgtctcgtg   1140

tttgacggcg gcgagggtgc ggcggtcaac gcgtcattga tcgacttagg gcaccgtttc   1200

cgactcatcg tcaatgaagt cgatgcggtg aaaccggaac acgacctgcc gaaattacca   1260

gtcgcccgca ttttatggaa gcctcgcccg tcgttccgcg actccgctga agcatggatt   1320

ttagctggcg gcgcccacca tacggtgttc tcatttgcgg ttacaacaga acagctgcaa   1380

gactttgcgg aaattgcagg gattgaactg gtcgtgatca atgaacatac gtccgtctcg   1440

tccttcaaaa atgaactgaa atggaacgaa gtgttttggc gggggcggta a            1491


<210>  6
<211>  1488
<212>  DNA
<213>  Artificial

<220>
<223>  mutated L-arabinose isomerase from G. stearothermophilus

<400>  6
atgtcattac gtccttatga attttggttt gtaacgggaa gccagcactt gtacggagaa     60

gaagcattaa agcaagttga agagcattca agcaaaattg tcaatgagct gaatcaagat    120

tcagtgttcc cgttcccact tgttttcaaa tcagttgtca caacgccaga ggaaatccgg    180

cgcgttttcc ttgaggcgaa tgcgagcgaa caatgcgctg gggtcatcac ttggctgcat    240

acattctcgc cagcgaagag ctggattggc ggccttttgg agctgcgaaa accgttattg    300

catcttcaca ctcaatttaa ccgtgatatt ccgtgggaca gcatcgatat ggactttatg    360

aacttaaacc aatcggctca cggtgaccgg gaatacggat ttatcggcgc gagactgggc    420

gtggcccgga aagtggtggt cgggcactgg gaagacccag aagtccgcga gcggctggcg    480

aaatggatgc ggacggctgt cgcgtttgcg gaaagccgca acctaaaagt ggctcgtttt    540

ggcgacaaca tgcgtgaagt ggctgtgacg gaaggggaca aagtcggagc gcaaattcaa    600

ttcggctggt cggtcaacac ctatggcatc ggggatttgg tgcaatacat ccgcgatgtt    660

tctgaacaaa aagtgaacga gttgctcgat gaatacgagg agctgtacga cattgtaccc    720

gccggccgcc aagaagggcc cgttcgcgaa tcaattcgtg aacaggcgcg gattgaactc    780

gggctgaaag cctttttgca ggatgggaac tttaccgctt tcacgacgac gttcgaggac    840

ttgcatgggc tgaagcagct cccgggactt gccgttcagc gactcctggc ggaaggatac    900

ggttttggag gcgagggaga ctggaaaacg gctgcgcttg tccggctact gaaagtgctg    960

gctgatggaa aagggacgtc atttatggaa gattacacgt accaccttga gccgggtaat   1020

gaaatgattc ttggcgccca catgttggaa gtatgcccga ctattgcagc gacccggccg   1080

cgaatcgaag tccatccgct ttccatcggt ggaaaagaag atccagcccg tctcgtgttt   1140

gacggcggcg agggtgcggc ggtcaacgcg tcattgatcg acttagggca ccgtttccga   1200

ctcatcgtca atgaagtcga tgcggtgaaa ccggaacacg acatgccgaa attaccagtc   1260

gcccgcattt tatggaagcc tcgcccgtcg ctccgcgact ccgctgaagc atggatttta   1320

gctggcggcg cccaccatac gtgcttctca tttgcggtta caacagaaca gctgcaagac   1380

tttgcggaaa tggcagggat tgaatgtgtc gtgatcaatg aacatacgtc cgtctcgtcc   1440

ttcaaaaatg aactgaaatg gaacgaagtg ttttggcggg ggcggtaa                1488


<210>  7
<211>  1488
<212>  DNA
<213>  Artificial

<220>
<223>  mutated L-arabinose isomerase from G. stearothermophilus

<400>  7
atgtcattac gtccttatga attttggttt gtaacgggaa gccagcactt gtacggagaa     60

gaagcattaa agcaagttga agagcattca agcaaaattg tcaatgagct gaatcaagat    120

tcagtgttcc cgttcccact tgttttcaaa tcagttgtca caacgccaga ggaaatccgg    180

cgcgttttcc ttgaggcgaa tgcgagcgaa caatgcgctg gggtcatcac ttggctgcat    240

acattctcgc cagcgaagag ctggattggc ggccttttgg agctgcgaaa accgttattg    300

catcttcaca ctcaatttaa ccgtgatatt ccgtgggaca gcatcgatat ggactttatg    360

aacttaaacc aatcggctca cggtgaccgg gaatacggat ttatcggcgc gagactgggc    420

gtggcccgga aagtggtggt cgggcactgg gaagacccag aagtccgcga gcggctggcg    480

aaatggatgc ggacggctgt cgcgtttgcg gaaagccgca acctaaaagt ggctcgtttt    540

ggcgacaaca tgcgtgaagt ggctgtgacg gaaggggaca aagtcggagc gcaaattcaa    600

ttcggctggt cggtcaacac ctatggcatc ggggatttgg tgcaatacat ccgcgatgtt    660

tctgaacaaa aagtgaacga gttgctcgat gaatacgagg agctgtacga cattgtaccc    720

gccggccgcc aagaagggcc cgttcgcgaa tcaattcgtg aacaggcgcg gattgaactc    780

gggctgaaag cctttttgca ggatgggaac tttaccgctt tcacgacgac gttcgaggac    840

ttgcatggga tgaagcagct gccgggactt gccgttcagc gactcatggc ggaaggatac    900

ggttttggag gcgagggaga ctggaaaacg gctgcgcttg tccggctaat gaaagtgatg    960

gctgatggaa aagggacgtc atttatggaa gattacacgt accaccttga gccgggtaat   1020

gaaatgattc ttggcgccca catgttggaa gtatgcccga ctattgcagc gacccggccg   1080

cgaatcgaag tccatccgct ttccatcggt ggaaaagaag atccagcccg tctcgtgttt   1140

gacggcggcg agggtgcggc ggtcaacgcg tcattgatcg acttagggca ccgtttccga   1200

ctcatcgtca atgaagtcga tgcggtgaaa ccggaacacg acatgccgaa attaccagtc   1260

gcccgcattt tatggaagcc tcgcccgtcg ctccgcgact ccgctgaagc atggatttta   1320

gctggcggcg cccaccatac gtgcttctca tttgcggtta caacagaaca gctgcaagac   1380

tttgcggaaa tggcagggat tgaatgtgtc gtgatcaatg aacatacgtc cgtctcgtcc   1440

ttcaaaaatg aactgaaatg gaacgaagtg ttttggcggg ggcggtaa                1488


<210>  8
<211>  495
<212>  PRT
<213>  Artificial

<220>
<223>  mutated L-arabinose isomerase from G. stearothermophilus

<400>  8

Met Ser Leu Arg Pro Tyr Glu Phe Trp Phe Val Thr Gly Ser Gln His 
1               5                   10                  15      


Leu Tyr Gly Glu Glu Ala Leu Lys Gln Val Glu Glu His Ser Ser Lys 
            20                  25                  30          


Ile Val Asn Glu Leu Asn Gln Asp Ser Val Phe Pro Phe Pro Leu Val 
        35                  40                  45              


Phe Lys Ser Val Val Thr Thr Pro Glu Glu Ile Arg Arg Val Phe Leu 
    50                  55                  60                  


Glu Ala Asn Ala Ser Glu Gln Cys Ala Gly Val Ile Thr Trp Leu His 
65                  70                  75                  80  


Thr Phe Ser Pro Ala Lys Ser Trp Ile Gly Gly Leu Leu Glu Leu Arg 
                85                  90                  95      


Lys Pro Leu Leu His Leu His Thr Gln Phe Asn Arg Asp Ile Pro Trp 
            100                 105                 110         


Asp Ser Ile Asp Met Asp Phe Met Asn Leu Asn Gln Ser Ala His Gly 
        115                 120                 125             


Asp Arg Glu Tyr Gly Phe Ile Gly Ala Arg Leu Gly Val Ala Arg Lys 
    130                 135                 140                 


Val Val Val Gly His Trp Glu Asp Pro Glu Val Arg Glu Arg Leu Ala 
145                 150                 155                 160 


Lys Trp Met Arg Thr Ala Val Ala Phe Ala Glu Ser Arg Asn Leu Lys 
                165                 170                 175     


Val Ala Arg Phe Gly Asp Asn Met Arg Glu Val Ala Val Thr Glu Gly 
            180                 185                 190         


Asp Lys Val Gly Ala Gln Ile Gln Phe Gly Trp Ser Val Asn Thr Tyr 
        195                 200                 205             


Gly Ile Gly Asp Leu Val Gln Tyr Ile Arg Asp Val Ser Glu Gln Lys 
    210                 215                 220                 


Val Asn Glu Leu Leu Asp Glu Tyr Glu Glu Leu Tyr Asp Ile Val Pro 
225                 230                 235                 240 


Ala Gly Arg Gln Glu Gly Pro Val Arg Glu Ser Ile Arg Glu Gln Ala 
                245                 250                 255     


Arg Ile Glu Leu Gly Leu Lys Ala Phe Leu Gln Asp Gly Asn Phe Thr 
            260                 265                 270         


Ala Phe Thr Thr Thr Phe Glu Asp Leu His Gly Leu Lys Gln Leu Pro 
        275                 280                 285             


Gly Leu Ala Val Gln Arg Leu Leu Ala Glu Gly Tyr Gly Phe Gly Gly 
    290                 295                 300                 


Glu Gly Asp Trp Lys Thr Ala Ala Leu Val Arg Leu Leu Lys Val Leu 
305                 310                 315                 320 


Ala Asp Gly Lys Gly Thr Ser Phe Met Glu Asp Tyr Thr Tyr His Leu 
                325                 330                 335     


Glu Pro Gly Asn Glu Leu Ile Leu Gly Ala His Met Leu Glu Val Asp 
            340                 345                 350         


Pro Thr Ile Ala Ala Thr Arg Pro Arg Ile Glu Val His Pro Leu Ser 
        355                 360                 365             


Ile Gly Gly Lys Glu Asp Pro Val Arg Leu Val Phe Asp Gly Gly Glu 
    370                 375                 380                 


Gly Ala Ala Val Asn Ala Ser Leu Ile Asp Leu Gly His Arg Phe Arg 
385                 390                 395                 400 


Leu Ile Val Asn Glu Val Asp Ala Val Lys Pro Glu His Asp Leu Pro 
                405                 410                 415     


Lys Leu Pro Val Ala Arg Ile Leu Trp Lys Pro Arg Pro Ser Phe Arg 
            420                 425                 430         


Asp Ser Ala Glu Ala Trp Ile Leu Ala Gly Gly Ala His His Thr Val 
        435                 440                 445             


Phe Ser Phe Ala Val Thr Thr Glu Gln Leu Gln Asp Phe Ala Glu Ile 
    450                 455                 460                 


Ala Gly Ile Glu Leu Val Val Ile Asn Glu His Thr Ser Val Ser Ser 
465                 470                 475                 480 


Phe Lys Asn Glu Leu Lys Trp Asn Glu Val Phe Trp Arg Gly Arg 
                485                 490                 495 


