                         SEQUENCE LISTING

<110>  Oncolytics Biotech Inc.
 
<120>  Protecting Modified Viruses from Neutralizing Antibodies Using 
       the Reovirus Sigma-1 Protein

<130>  32894-2016

<150>  61/640,403
<151>  2012-04-30

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  455
<212>  PRT
<213>  reovirus

<400>  1

Met Asp Pro Arg Leu Arg Glu Glu Val Val Arg Leu Ile Ile Ala Leu 
1               5                   10                  15      


Thr Ser Asp Asn Gly Ala Ser Leu Ser Lys Gly Leu Glu Ser Arg Val 
            20                  25                  30          


Ser Ala Leu Glu Lys Thr Ser Gln Ile His Ser Asp Thr Ile Leu Arg 
        35                  40                  45              


Ile Thr Gln Gly Leu Asp Asp Ala Asn Lys Arg Ile Ile Ala Leu Glu 
    50                  55                  60                  


Gln Ser Arg Asp Asp Leu Val Ala Ser Val Ser Asp Ala Gln Leu Ala 
65                  70                  75                  80  


Ile Ser Arg Leu Glu Ser Ser Ile Gly Ala Leu Gln Thr Val Val Asn 
                85                  90                  95      


Gly Leu Asp Ser Ser Val Thr Gln Leu Gly Ala Arg Val Gly Gln Leu 
            100                 105                 110         


Glu Thr Gly Leu Ala Glu Leu Arg Val Asp His Asp Asn Leu Val Ala 
        115                 120                 125             


Arg Val Asp Thr Ala Glu Arg Asn Ile Gly Ser Leu Thr Thr Glu Leu 
    130                 135                 140                 


Ser Thr Leu Thr Leu Arg Val Thr Ser Ile Gln Ala Asp Phe Glu Ser 
145                 150                 155                 160 


Arg Ile Ser Thr Leu Glu Arg Thr Ala Val Thr Ser Ala Gly Ala Pro 
                165                 170                 175     


Leu Ser Ile Arg Asn Asn Arg Met Thr Met Gly Leu Asn Asp Gly Leu 
            180                 185                 190         


Thr Leu Ser Gly Asn Asn Leu Ala Ile Arg Leu Pro Gly Asn Thr Gly 
        195                 200                 205             


Leu Asn Ile Gln Asn Gly Gly Leu Gln Phe Arg Phe Asn Thr Asp Gln 
    210                 215                 220                 


Phe Gln Ile Val Asn Asn Asn Leu Thr Leu Lys Thr Thr Val Phe Asp 
225                 230                 235                 240 


Ser Ile Asn Ser Arg Ile Gly Ala Thr Glu Gln Ser Tyr Val Ala Ser 
                245                 250                 255     


Ala Val Thr Pro Leu Arg Leu Asn Ser Ser Thr Lys Val Leu Asp Met 
            260                 265                 270         


Leu Ile Asp Ser Ser Thr Leu Glu Ile Asn Ser Ser Gly Gln Leu Thr 
        275                 280                 285             


Val Arg Ser Thr Ser Pro Asn Leu Arg Tyr Pro Ile Ala Asp Val Ser 
    290                 295                 300                 


Gly Gly Ile Gly Met Ser Pro Asn Tyr Arg Phe Arg Gln Ser Met Trp 
305                 310                 315                 320 


Ile Gly Ile Val Ser Tyr Ser Gly Ser Gly Leu Asn Trp Arg Val Gln 
                325                 330                 335     


Val Asn Ser Asp Ile Phe Ile Val Asp Asp Tyr Ile His Ile Cys Leu 
            340                 345                 350         


Pro Ala Phe Asp Gly Phe Ser Ile Ala Asp Gly Gly Asp Leu Ser Leu 
        355                 360                 365             


Asn Phe Val Thr Gly Leu Leu Pro Pro Leu Leu Thr Gly Asp Thr Glu 
    370                 375                 380                 


Pro Ala Phe His Asn Asp Val Val Thr Tyr Gly Ala Gln Thr Val Ala 
385                 390                 395                 400 


Ile Gly Leu Ser Ser Gly Gly Ala Pro Gln Tyr Met Ser Lys Asn Leu 
                405                 410                 415     


Trp Val Glu Gln Trp Gln Asp Gly Val Leu Arg Leu Arg Val Glu Gly 
            420                 425                 430         


Gly Gly Ser Ile Thr His Ser Asn Ser Lys Trp Pro Ala Met Thr Val 
        435                 440                 445             


Ser Tyr Pro Arg Ser Phe Thr 
    450                 455 


<210>  2
<211>  455
<212>  PRT
<213>  reovirus

<400>  2

Met Asp Pro Arg Leu Arg Glu Glu Val Val Arg Leu Ile Ile Ala Leu 
1               5                   10                  15      


Thr Gly Asp Asn Gly Thr Ser Leu Ser Lys Gly Leu Glu Ser Arg Ile 
            20                  25                  30          


Ala Ala Leu Glu Lys Thr Ser Gln Ile His Ser Asp Thr Leu Leu Arg 
        35                  40                  45              


Ile Thr Gln Gly Leu Asp Asp Ala Asn Lys Arg Ile Ile Ala Leu Glu 
    50                  55                  60                  


Gln Ser Arg Asp Asp Leu Val Ala Ser Val Ser Asp Ala Gln Leu Ala 
65                  70                  75                  80  


Ile Ser Arg Leu Glu Asn Ser Val Gly Ala Leu Gln Thr Ala Ile Asn 
                85                  90                  95      


Gly Leu Asp Ser Ser Val Thr Gln Leu Gly Gly Arg Val Gly Gln Leu 
            100                 105                 110         


Glu Thr Gly Leu Ala Gly Leu Arg Ala Asp His Asp Asn Leu Ala Thr 
        115                 120                 125             


Arg Val Asp Thr Ala Glu Arg Asn Ile Gly Ser Leu Thr Thr Glu Leu 
    130                 135                 140                 


Ser Thr Leu Thr Leu Arg Val Thr Ser Ile Gln Ser Asp Phe Glu Ser 
145                 150                 155                 160 


Arg Ile Ala Thr Leu Glu Arg Thr Ala Val Thr Ser Ala Ala Ala Pro 
                165                 170                 175     


Leu Ser Ile Ser Asn Asn Arg Met Thr Met Gly Leu Asn Asp Gly Leu 
            180                 185                 190         


Thr Leu Ser Gly Asn Asn Leu Ala Ile Arg Leu Pro Gly Asn Thr Gly 
        195                 200                 205             


Leu Asn Ile Gln Asn Gly Gly Leu Gln Phe Arg Phe Asn Thr Asp Gln 
    210                 215                 220                 


Phe Gln Ile Val Asn Asn Asn Leu Thr Leu Lys Thr Thr Ile Phe Asp 
225                 230                 235                 240 


Pro Ile Asn Ser Arg Ile Ser Thr Ile Glu Gln Ser Tyr Val Val Ser 
                245                 250                 255     


Ala Val Thr Pro Leu Arg Leu Asn Ser Ser Thr Lys Val Leu Asp Met 
            260                 265                 270         


Leu Ile Asp Ser Ser Thr Leu Glu Ile Asn Ser Ser Gly Gln Leu Ala 
        275                 280                 285             


Val Lys Ser Thr Ser Pro Asn Leu Arg Tyr Pro Ile Ala Asp Val Ser 
    290                 295                 300                 


Gly Ser Ile Gly Val Ser Pro Asn Tyr Arg Phe Arg Gln Ser Met Trp 
305                 310                 315                 320 


Ile Gly Ile Val Ser Tyr Ser Gly Ser Gly Leu Ser Trp Arg Ile Gln 
                325                 330                 335     


Val Asn Ser Asp Ile Phe Ile Ala Asp Asp Tyr Ile His Ile Cys Leu 
            340                 345                 350         


Pro Ala Phe Asp Gly Phe Thr Ile Ala Asp Gly Gly Asp Leu Ser Leu 
        355                 360                 365             


Asn Phe Val Thr Gly Leu Leu Pro Pro Leu Leu Thr Gly Asp Thr Glu 
    370                 375                 380                 


Pro Ala Phe His Asn Asp Val Val Thr Tyr Gly Ala Arg Thr Ile Ala 
385                 390                 395                 400 


Ile Gly Leu Ser Ser Gly Gly Thr Pro Gln Tyr Ile Ser Lys Asn Leu 
                405                 410                 415     


Trp Val Glu Gln Trp Gln Asp Gly Val Leu Arg Leu Arg Val Glu Gly 
            420                 425                 430         


Gly Gly Leu Ile Thr His Ser Asn Ser Lys Trp Pro Ala Met Thr Ile 
        435                 440                 445             


Ser Tyr Pro Arg Ser Phe Thr 
    450                 455 


<210>  3
<211>  455
<212>  PRT
<213>  reovirus

<400>  3

Met Asp Pro Arg Leu Arg Glu Glu Val Val Arg Leu Ile Ile Ala Leu 
1               5                   10                  15      


Thr Ile Asp Asn Gly Ala Ser Leu Ser Lys Gly Leu Glu Ser Arg Val 
            20                  25                  30          


Ser Ala Leu Glu Lys Thr Ser Gln Ile His Ser Asp Thr Ile Leu Arg 
        35                  40                  45              


Ile Thr Gln Gly Leu Asp Asp Ala Asn Lys Arg Ile Ile Ala Leu Glu 
    50                  55                  60                  


Gln Ser Arg Asp Asp Leu Val Ala Ser Val Ser Asp Ala Gln Leu Ala 
65                  70                  75                  80  


Ile Ser Arg Leu Glu Ser Ser Ile Gly Ala Leu Gln Thr Val Val Asn 
                85                  90                  95      


Gly Leu Asp Ser Ser Val Thr Gln Leu Gly Ala Arg Val Gly Gln Leu 
            100                 105                 110         


Glu Thr Gly Leu Ala Glu Leu Arg Val Asp His Asp Asn Leu Val Ala 
        115                 120                 125             


Arg Val Asp Thr Ala Glu Arg Asn Ile Gly Ser Leu Thr Thr Glu Leu 
    130                 135                 140                 


Ser Thr Leu Thr Leu Arg Val Thr Ser Ile Gln Ala Asp Phe Glu Ser 
145                 150                 155                 160 


Arg Ile Ser Thr Leu Glu Arg Thr Ala Val Thr Ser Ala Gly Ala Pro 
                165                 170                 175     


Leu Ser Ile Arg Asn Asn Arg Met Thr Met Gly Leu Asn Asp Gly Leu 
            180                 185                 190         


Thr Leu Ser Gly Asn Asn Leu Ala Ile Arg Leu Pro Gly Asn Thr Gly 
        195                 200                 205             


Leu Asn Ile Gln Asn Gly Gly Leu Gln Phe Arg Phe Asn Thr Asp Gln 
    210                 215                 220                 


Phe Gln Ile Val Asn Asn Asn Leu Thr Leu Lys Thr Thr Val Phe Asp 
225                 230                 235                 240 


Ser Ile Asn Ser Arg Ile Gly Ala Thr Glu Gln Ser Tyr Val Ala Ser 
                245                 250                 255     


Ala Val Thr Pro Leu Arg Leu Asn Ser Ser Thr Lys Val Leu Asp Met 
            260                 265                 270         


Leu Ile Asp Ser Ser Thr Leu Glu Ile Asn Ser Ser Gly Gln Leu Thr 
        275                 280                 285             


Val Arg Ser Thr Ser Pro Asn Leu Arg Tyr Pro Ile Ala Asp Val Ser 
    290                 295                 300                 


Gly Gly Ile Gly Met Ser Pro Asn Tyr Arg Phe Arg Gln Ser Met Trp 
305                 310                 315                 320 


Ile Gly Ile Val Ser Tyr Ser Gly Ser Gly Leu Asn Trp Arg Val Gln 
                325                 330                 335     


Val Asn Ser Asp Ile Phe Ile Val Asp Asp Tyr Ile His Ile Cys Leu 
            340                 345                 350         


Pro Ala Phe Asp Gly Phe Ser Ile Ala Asp Gly Gly Asp Leu Ser Leu 
        355                 360                 365             


Asn Phe Val Thr Gly Leu Leu Pro Pro Leu Leu Thr Gly Asp Thr Glu 
    370                 375                 380                 


Pro Ala Phe His Asn Asp Val Val Thr Tyr Gly Ala Gln Thr Val Ala 
385                 390                 395                 400 


Ile Gly Leu Ser Ser Gly Gly Ala Pro Gln Tyr Met Ser Lys Asn Leu 
                405                 410                 415     


Trp Val Glu Gln Trp Gln Asp Gly Val Leu Arg Leu Arg Val Glu Gly 
            420                 425                 430         


Gly Gly Ser Ile Thr His Ser Asn Ser Lys Trp Pro Ala Met Thr Val 
        435                 440                 445             


Ser Tyr Pro Arg Ser Phe Thr 
    450                 455 


<210>  4
<211>  1416
<212>  DNA
<213>  reovirus

<400>  4
gctattggtc ggatggatcc tcgcctacgt gaagaagtag tacggctgat aatcgcatta       60

acgagtgata atggagcatc actgtcaaaa gggcttgaat caagggtctc ggcgctcgag      120

aagacgtctc aaatacactc tgatactatc ctccggatca cccagggact cgatgatgca      180

aacaaacgaa tcatcgctct tgagcaaagt cgggatgact tggttgcatc agtcagtgat      240

gctcaacttg caatctccag attggaaagc tctatcggag ccctccaaac agttgtcaat      300

ggacttgatt cgagtgttac ccagttgggt gctcgagtgg gacaacttga gacaggactt      360

gcagagctac gcgttgatca cgacaatctc gttgcgagag tggatactgc agaacgtaac      420

attggatcat tgaccactga gctatcaact ctgacgttac gagtaacatc catacaagcg      480

gatttcgaat ctaggatatc cacgttagag cgcacggcgg tcactagcgc gggagctccc      540

ctctcaatcc gtaataaccg tatgaccatg ggattaaatg atggactcac gttgtcaggg      600

aataatctcg ccatccgatt gccaggaaat acgggtctga atattcaaaa tggtggactt      660

cagtttcgat ttaatactga tcaattccag atagttaata ataacttgac tctcaagacg      720

actgtgtttg attctatcaa ctcaaggata ggcgcaactg agcaaagtta cgtggcgtcg      780

gcagtgactc ccttgagatt aaacagtagc acgaaggtgc tggatatgct aatagacagt      840

tcaacacttg aaattaattc tagtggacag ctaactgtta gatcgacatc cccgaatttg      900

aggtatccga tagctgatgt tagcggcggt atcggaatga gtccaaatta taggtttagg      960

cagagcatgt ggataggaat tgtctcctat tctggtagtg ggctgaattg gagggtacag     1020

gtgaactccg acatttttat tgtagatgat tacatacata tatgtcttcc agcttttgac     1080

ggtttctcta tagctgacgg tggagatcta tcgttgaact ttgttaccgg attgttacca     1140

ccgttactta caggagacac tgagcccgct tttcataatg acgtggtcac atatggagca     1200

cagactgtag ctatagggtt gtcgtcgggt ggtgcgcctc agtatatgag taagaatctg     1260

tgggtggagc agtggcagga tggagtactt cggttacgtg ttgagggggg tggctcaatt     1320

acgcactcaa acagtaagtg gcctgccatg accgtttcgt acccgcgtag tttcacgtga     1380

ggatcagacc accccgcggc actggggcat ttcatc                               1416


<210>  5
<211>  1368
<212>  DNA
<213>  reovirus

<400>  5
atggatcctc gcttacgtga agaagtagtg cgattaataa ttgcattgac gggcgataat       60

ggaacatcat tgtcaaaagg gcttgaatca aggattgcag cgctcgaaaa gacgtcccaa      120

atacactctg atactctcct caggatcacc caaggactcg atgatgcaaa taaacgaatc      180

atcgctcttg aacaaagtag ggacgacctg gttgcatcag tcagtgatgc acaacttgca      240

atctccagat tggaaaactc tgtcggagcc ctccaaacag ctatcaatgg acttgattcg      300

agtgttaccc agctgggtgg tcgagtggga cagcttgaga caggacttgc aggattacgc      360

gctgaccacg acaatctcgc tacgagagtg gatactgcgg aacgtaacat tggatcattg      420

accactgagc tatctacact tacattacgt gtgacctcca tacaatcgga tttcgaatct      480

cgaatagcca cattagagcg tacagcggtc actagcgcag cggcacctct ctcgattagc      540

aataatcgta tgactatggg cttgaatgac ggattaacat tgtcgggaaa taatcttgcc      600

attcgactgc caggaaacac aggtctgaat attcagaatg gtggacttca atttcggttt      660

aatactgacc agttccagat agttaacaat aacttgactc tcaagacaac tatctttgat      720

ccaatcaact ccagaataag tacgattgaa caaagttacg tggtatcagc agtgactccc      780

ttgaggctta atagcagtac caaggtgttg gacatgctca tagatagctc gactcttgaa      840

attaactcta gcggacaatt agccgtgaag tcgacatcgc ctaacttaag atatccaatt      900

gctgatgtta gtggtagtat aggagtgagc cctaactata ggttcaggca gagcatgtgg      960

ataggaatag tttcatactc tggtagtggg ttgagttgga ggatacaggt gaattctgac     1020

atcttcattg ctgatgatta tatacacata tgtcttccag cttttgatgg tttcacaata     1080

gccgacggtg gagatctatc gttaaacttt gtcacaggat tgttgccacc gctactcacc     1140

ggggatactg agccggcttt ccataatgat gtagttacgt atggggcacg gactatagct     1200

atagggttgt cgtcaggtgg tacgcctcag tatataagta agaacttgtg ggtggagcag     1260

tggcaggatg gagtactacg gttgcgtgtt gaaggagggg gcttgatcac gcattcgaat     1320

agtaaatggc ctgccatgac catttcttat ccacgcagtt tcacgtga                  1368


<210>  6
<211>  1416
<212>  DNA
<213>  reovirus

<400>  6
gctattggtc ggatggatcc tcgcctacgt gaagaagtag tacggctgat aatcgcatta       60

acgattgata atggagcatc actgtcaaaa gggcttgaat caagggtctc ggcgctcgag      120

aagacgtctc aaatacactc tgatactatc ctccggatca cccagggact cgatgatgca      180

aacaaacgaa tcatcgctct tgagcaaagt cgggatgact tggttgcatc agtcagtgat      240

gctcaacttg caatctccag attggaaagc tctatcggag ccctccaaac agttgtcaat      300

ggacttgatt cgagtgttac ccagttgggt gctcgagtgg gacaacttga gacaggactt      360

gcagagctac gcgttgatca cgacaatctc gttgcgagag tggatactgc agaacgtaac      420

attggatcat tgaccactga gctatcaact ctgacgttac gagtaacatc catacaagcg      480

gatttcgaat ctaggatatc cacgttagag cgcacggcgg tcactagcgc gggagctccc      540

ctctcaatcc gtaataaccg tatgaccatg ggattaaatg atggactcac gttgtcaggg      600

aataatctcg ccatccgatt gccaggaaat acgggtctga atattcaaaa tggtggactt      660

cagtttcgat ttaatactga tcaattccag atagttaata ataacttgac tctcaagacg      720

actgtgtttg attctatcaa ctcaaggata ggcgcaactg agcaaagtta cgtggcgtcg      780

gcagtgactc ccttgagatt aaacagtagc acgaaggtgc tggatatgct aatagacagt      840

tcaacacttg aaattaattc tagtggacag ctaactgtta gatcgacatc cccgaatttg      900

aggtatccga tagctgatgt tagcggcggt atcggaatga gtccaaatta taggtttagg      960

cagagcatgt ggataggaat tgtctcctat tctggtagtg ggctgaattg gagggtacag     1020

gtgaactccg acatttttat tgtagatgat tacatacata tatgtcttcc agcttttgac     1080

ggtttctcta tagctgacgg tggagatcta tcgttgaact ttgttaccgg attgttacca     1140

ccgttactta caggagacac tgagcccgct tttcataatg acgtggtcac atatggagca     1200

cagactgtag ctatagggtt gtcgtcgggt ggtgcgcctc agtatatgag taagaatctg     1260

tgggtggagc agtggcagga tggagtactt cggttacgtg ttgagggggg tggctcaatt     1320

acgcactcaa acagtaagtg gcctgccatg accgtttcgt acccgcgtag tttcacgtga     1380

ggatcagacc accccgcggc actggggcat ttcatc                               1416


