                         SEQUENCE LISTING

<110>  UCB Pharma S.A
 
<120>  Recombinant Bacterial Host Cell for Protein Expression

<130>  G0164-WO

<150>  GB1208367.1
<151>  2012-05-14

<160>  82    

<170>  PatentIn version 3.5

<210>  1
<211>  2049
<212>  DNA
<213>  Escherichia coli

<400>  1
atgaacatgt tttttaggct taccgcgtta gctggcctgc ttgcaatagc aggccagacc       60

ttcgctgtag aagatatcac gcgtgctgat caaattccgg tattaaagga agagacgcag      120

catgcgacgg taagtgagcg cgtaacgtcg cgcttcaccc gttctcatta tcgccagttc      180

gacctcgatc aggcattttc ggccaaaatc tttgaccgct acctgaatct gctcgattac      240

agccacaacg tgctgctggc aagcgatgtt gaacagttcg cgaaaaagaa aaccgagtta      300

ggcgatgaac tgcgttcagg caaactcgac gttttctacg atctctacaa tctggcgcaa      360

aagcgccgtt ttgagcgtta ccagtacgct ttgtcggtac tggaaaagcc gatggatttc      420

accggcaacg acacttataa ccttgaccgc agcaaagcgc cctggccgaa aaacgaggct      480

gagttgaacg cgctgtggga cagtaaagtc aaattcgacg agttaagcct gaagctgaca      540

ggaaaaacgg ataaagaaat tcgtgaaacc ctgactcgcc gctacaaatt tgccattcgt      600

cgtctggcgc aaaccaacag cgaagatgtt ttctcgctgg caatgacggc gtttgcgcgt      660

gaaatcgacc cgcataccaa ctatctttcc ccgcgtaata ccgaacagtt caacactgaa      720

atgagtttgt cgctggaagg tattggcgca gtgctgcaaa tggatgatga ctacaccgtt      780

atcaattcga tggtggcagg tggtccggca gcgaagagta aagctatcag cgttggtgac      840

aaaattgtcg gtgttggtca aacaggcaag ccgatggttg acgtgattgg ctggcgtctt      900

gatgatgtgg ttgccttaat taaagggccg aagggcagta aagttcgtct ggaaatttta      960

cctgctggta aagggaccaa gacccgtact gtaacgttga cccgtgaacg tattcgtctc     1020

gaagaccgcg cggttaaaat gtcggtgaag accgtcggta aagagaaagt cggcgtgctg     1080

gatattccgg gcttctatgt gggtttgaca gacgatgtca aagtgcaact gcagaaactg     1140

gaaaaacaga atgtcagcag cgtcatcatc gacctgcgta gcaatggcgg tggggcgtta     1200

actgaagccg tatcgctctc cggtctgttt attcctgcgg gtcccattgt tcaggtccgc     1260

gataacaacg gcaaggttcg tgaagatagc gataccgacg gacaggtttt ctataaaggc     1320

ccgctggtgg tgctggttga ccgcttcagt gcttcggctt cagaaatctt tgccgcggca     1380

atgcaggatt acggtcgtgc gctggttgtg ggtgaaccga cgtttggtaa aggcaccgtt     1440

cagcaatacc gttcattgaa ccgtatttac gatcagatgt tacgtcctga atggccagcg     1500

ctgggttctg tgcagtacac gatccagaaa ttctatcgcg ttaacggcgg cagtacgcaa     1560

cgtaaaggcg taacgccaga catcatcatg ccgacgggta atgaagaaac ggaaacgggt     1620

gagaaattcg aagataacgc gctgccgtgg gatagcattg atgccgcgac ttatgtgaaa     1680

tcaggagatt taacggcctt tgaaccggag ctgctgaagg aacataatgc gcgtatcgcg     1740

aaagatcctg agttccagaa catcatgaag gatatcgcgc gcttcaacgc tatgaaggac     1800

aagcgcaata tcgtttctct gaattacgct gtgcgtgaga aagagaataa tgaagatgat     1860

gcgacgcgtc tggcgcgttt gaacgaacgc tttaaacgcg aaggtaaacc ggagttgaag     1920

aaactggatg atctaccgaa agattaccag gagccggatc cttatctgga tgagacggtg     1980

aatatcgcac tcgatctggc gaagcttgaa aaagccagac ccgcggaaca acccgctccc     2040

gtcaagtaa                                                             2049


<210>  2
<211>  680
<212>  PRT
<213>  Escherichia coli

<400>  2

Met Phe Phe Arg Leu Thr Ala Leu Ala Gly Leu Leu Ala Ile Ala Gly 
1               5                   10                  15      


Gln Thr Phe Ala Val Glu Asp Ile Thr Arg Ala Asp Gln Ile Pro Val 
            20                  25                  30          


Leu Lys Glu Glu Thr Gln His Ala Thr Val Ser Glu Arg Val Thr Ser 
        35                  40                  45              


Arg Phe Thr Arg Ser His Tyr Arg Gln Phe Asp Leu Asp Gln Ala Phe 
    50                  55                  60                  


Ser Ala Lys Ile Phe Asp Arg Tyr Leu Asn Leu Leu Asp Tyr Ser His 
65                  70                  75                  80  


Asn Val Leu Leu Ala Ser Asp Val Glu Gln Phe Ala Lys Lys Lys Thr 
                85                  90                  95      


Glu Leu Gly Asp Glu Leu Arg Ser Gly Lys Leu Asp Val Phe Tyr Asp 
            100                 105                 110         


Leu Tyr Asn Leu Ala Gln Lys Arg Arg Phe Glu Arg Tyr Gln Tyr Ala 
        115                 120                 125             


Leu Ser Val Leu Glu Lys Pro Met Asp Phe Thr Gly Asn Asp Thr Tyr 
    130                 135                 140                 


Asn Leu Asp Arg Ser Lys Ala Pro Trp Pro Lys Asn Glu Ala Glu Leu 
145                 150                 155                 160 


Asn Ala Leu Trp Asp Ser Lys Val Lys Phe Asp Glu Leu Ser Leu Lys 
                165                 170                 175     


Leu Thr Gly Lys Thr Asp Lys Glu Ile Arg Glu Thr Leu Thr Arg Arg 
            180                 185                 190         


Tyr Lys Phe Ala Ile Arg Arg Leu Ala Gln Thr Asn Ser Glu Asp Val 
        195                 200                 205             


Phe Ser Leu Ala Met Thr Ala Phe Ala Arg Glu Ile Asp Pro His Thr 
    210                 215                 220                 


Asn Tyr Leu Ser Pro Arg Asn Thr Glu Gln Phe Asn Thr Glu Met Ser 
225                 230                 235                 240 


Leu Ser Leu Glu Gly Ile Gly Ala Val Leu Gln Met Asp Asp Asp Tyr 
                245                 250                 255     


Thr Val Ile Asn Ser Met Val Ala Gly Gly Pro Ala Ala Lys Ser Lys 
            260                 265                 270         


Ala Ile Ser Val Gly Asp Lys Ile Val Gly Val Gly Gln Thr Gly Lys 
        275                 280                 285             


Pro Met Val Asp Val Ile Gly Trp Arg Leu Asp Asp Val Val Ala Leu 
    290                 295                 300                 


Ile Lys Gly Pro Lys Gly Ser Lys Val Arg Leu Glu Ile Leu Pro Ala 
305                 310                 315                 320 


Gly Lys Gly Thr Lys Thr Arg Thr Val Thr Leu Thr Arg Glu Arg Ile 
                325                 330                 335     


Arg Leu Glu Asp Arg Ala Val Lys Met Ser Val Lys Thr Val Gly Lys 
            340                 345                 350         


Glu Lys Val Gly Val Leu Asp Ile Pro Gly Phe Tyr Val Gly Leu Thr 
        355                 360                 365             


Asp Asp Val Lys Val Gln Leu Gln Lys Leu Glu Lys Gln Asn Val Ser 
    370                 375                 380                 


Ser Val Ile Ile Asp Leu Arg Ser Asn Gly Gly Gly Ala Leu Thr Glu 
385                 390                 395                 400 


Ala Val Ser Leu Ser Gly Leu Phe Ile Pro Ala Gly Pro Ile Val Gln 
                405                 410                 415     


Val Arg Asp Asn Asn Gly Lys Val Arg Glu Asp Ser Asp Thr Asp Gly 
            420                 425                 430         


Gln Val Phe Tyr Lys Gly Pro Leu Val Val Leu Val Asp Arg Phe Ser 
        435                 440                 445             


Ala Ser Ala Ser Glu Ile Phe Ala Ala Ala Met Gln Asp Tyr Gly Arg 
    450                 455                 460                 


Ala Leu Val Val Gly Glu Pro Thr Phe Gly Lys Gly Thr Val Gln Gln 
465                 470                 475                 480 


Tyr Arg Ser Leu Asn Arg Ile Tyr Asp Gln Met Leu Arg Pro Glu Trp 
                485                 490                 495     


Pro Ala Leu Gly Ser Val Gln Tyr Thr Ile Gln Lys Phe Tyr Arg Val 
            500                 505                 510         


Asn Gly Gly Ser Thr Gln Arg Lys Gly Val Thr Pro Asp Ile Ile Met 
        515                 520                 525             


Pro Thr Gly Asn Glu Glu Thr Glu Thr Gly Glu Lys Phe Glu Asp Asn 
    530                 535                 540                 


Ala Leu Pro Trp Asp Ser Ile Asp Ala Ala Thr Tyr Val Lys Ser Gly 
545                 550                 555                 560 


Asp Leu Thr Ala Phe Glu Pro Glu Leu Leu Lys Glu His Asn Ala Arg 
                565                 570                 575     


Ile Ala Lys Asp Pro Glu Phe Gln Asn Ile Met Lys Asp Ile Ala Arg 
            580                 585                 590         


Phe Asn Ala Met Lys Asp Lys Arg Asn Ile Val Ser Leu Asn Tyr Ala 
        595                 600                 605             


Val Arg Glu Lys Glu Asn Asn Glu Asp Asp Ala Thr Arg Leu Ala Arg 
    610                 615                 620                 


Leu Asn Glu Arg Phe Lys Arg Glu Gly Lys Pro Glu Leu Lys Lys Leu 
625                 630                 635                 640 


Asp Asp Leu Pro Lys Asp Tyr Gln Glu Pro Asp Pro Tyr Leu Asp Glu 
                645                 650                 655     


Thr Val Asn Ile Ala Leu Asp Leu Ala Lys Leu Glu Lys Ala Arg Pro 
            660                 665                 670         


Ala Glu Gln Pro Ala Pro Val Lys 
        675                 680 


<210>  3
<211>  2048
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutated knockout Tsp gene

<400>  3
atgaattcgt ttttaggctt accgcgttag ctggcctgct tgcaatagca ggccagacat       60

taattgtaga agatatcacg cgtgctgatc aaattccggt attaaaggaa gagacgcagc      120

atgcgacggt aagtgagcgc gtaacgtcgc gcttcacccg ttctcattat cgccagttcg      180

acctcgatca ggcattttcg gccaaaatct ttgaccgcta cctgaatctg ctcgattaca      240

gccacaacgt gctgctggca agcgatgttg aacagttcgc gaaaaagaaa accgagttag      300

gcgatgaact gcgttcaggc aaactcgacg ttttctacga tctctacaat ctggcgcaaa      360

agcgccgttt tgagcgttac cagtacgctt tgtcggtact ggaaaagccg atggatttca      420

ccggcaacga cacttataac cttgaccgca gcaaagcgcc ctggccgaaa aacgaggctg      480

agttgaacgc gctgtgggac agtaaagtca aattcgacga gttaagcctg aagctgacag      540

gaaaaacgga taaagaaatt cgtgaaaccc tgactcgccg ctacaaattt gccattcgtc      600

gtctggcgca aaccaacagc gaagatgttt tctcgctggc aatgacggcg tttgcgcgtg      660

aaatcgaccc gcataccaac tatctttccc cgcgtaatac cgaacagttc aacactgaaa      720

tgagtttgtc gctggaaggt attggcgcag tgctgcaaat ggatgatgac tacaccgtta      780

tcaattcgat ggtggcaggt ggtccggcag cgaagagtaa agctatcagc gttggtgaca      840

aaattgtcgg tgttggtcaa acaggcaagc cgatggttga cgtgattggc tggcgtcttg      900

atgatgtggt tgccttaatt aaagggccga agggcagtaa agttcgtctg gaaattttac      960

ctgctggtaa agggaccaag acccgtactg taacgttgac ccgtgaacgt attcgtctcg     1020

aagaccgcgc ggttaaaatg tcggtgaaga ccgtcggtaa agagaaagtc ggcgtgctgg     1080

atattccggg cttctatgtg ggtttgacag acgatgtcaa agtgcaactg cagaaactgg     1140

aaaaacagaa tgtcagcagc gtcatcatcg acctgcgtag caatggcggt ggggcgttaa     1200

ctgaagccgt atcgctctcc ggtctgttta ttcctgcggg tcccattgtt caggtccgcg     1260

ataacaacgg caaggttcgt gaagatagcg ataccgacgg acaggttttc tataaaggcc     1320

cgctggtggt gctggttgac cgcttcagtg cttcggcttc agaaatcttt gccgcggcaa     1380

tgcaggatta cggtcgtgcg ctggttgtgg gtgaaccgac gtttggtaaa ggcaccgttc     1440

agcaataccg ttcattgaac cgtatttacg atcagatgtt acgtcctgaa tggccagcgc     1500

tgggttctgt gcagtacacg atccagaaat tctatcgcgt taacggcggc agtacgcaac     1560

gtaaaggcgt aacgccagac atcatcatgc cgacgggtaa tgaagaaacg gaaacgggtg     1620

agaaattcga agataacgcg ctgccgtggg atagcattga tgccgcgact tatgtgaaat     1680

caggagattt aacggccttt gaaccggagc tgctgaagga acataatgcg cgtatcgcga     1740

aagatcctga gttccagaac atcatgaagg atatcgcgcg cttcaacgct atgaaggaca     1800

agcgcaatat cgtttctctg aattacgctg tgcgtgagaa agagaataat gaagatgatg     1860

cgacgcgtct ggcgcgtttg aacgaacgct ttaaacgcga aggtaaaccg gagttgaaga     1920

aactggatga tctaccgaaa gattaccagg agccggatcc ttatctggat gagacggtga     1980

atatcgcact cgatctggcg aagcttgaaa aagccagacc cgcggaacaa cccgctcccg     2040

tcaagtaa                                                              2048


<210>  4
<211>  2889
<212>  DNA
<213>  Escherichia coli

<400>  4
atgccccgca gcacctggtt caaagcatta ttgttgttag ttgccctttg ggcaccctta       60

agtcaggcag aaacgggatg gcagccgatt caggaaacca tccgtaaaag tgataaagat      120

aaccgccagt atcaggctat acgtctggat aacggtatgg tggtcttgct ggtttctgat      180

ccgcaggcag ttaaatcgct ctcggcgctg gtggtgcccg ttgggtcgct ggaagatccc      240

gaggcgtacc aggggctggc acattacctt gaacatatga gtctgatggg gtcgaaaaag      300

tacccgcagg ctgacagtct ggccgaatat ctcaaaatgc acggcggtag tcacaatgcc      360

agcactgcgc cgtatcgcac ggctttctat ctggaagttg agaacgacgc cttgcctggt      420

gcggtagacc gcctggccga tgctattgct gaacctttgc tcgacaagaa atatgccgaa      480

cgtgagcgta atgcggtgaa cgctgaatta accatggcgc gtacgcgtga cgggatgcgc      540

atggcacagg tcagcgcaga aaccattaac ccggcacacc ccggttcaaa gttttctggt      600

ggtaacctcg aaactttaag cgacaaacct ggtaatccgg tgcagcaggc gctgaaagat      660

ttccacgaga agtactattc cgccaatttg atgaaggcgg ttatttacag taataaaccg      720

ctgccggagt tggcaaaaat ggcggcggac acctttggtc gcgtgccgaa caaagagagc      780

aaaaaaccgg aaatcaccgt gccggtagtc accgacgcgc aaaagggcat tatcattcat      840

tacgtccctg cgctgccgcg taaagtgttg cgcgttgagt ttcgcatcga taacaactca      900

gcgaagttcc gtagtaaaac cgatgaattg attacctatc tgattggcaa tcgcagccca      960

ggtacacttt ctgactggct gcaaaagcag ggattagttg agggcattag cgccaactcc     1020

gatcctatcg tcaacggcaa cagcggcgta ttagcgatct ctgcgtcttt aaccgataaa     1080

ggcctggcta atcgcgatca ggttgtggcg gcaattttta gctatctcaa tctgttacgt     1140

gaaaaaggca ttgataaaca atacttcgat gaactggcga atgtgctgga tatcgacttc     1200

cgttatccgt cgatcacccg tgatatggat tacgtcgaat ggctggcaga taccatgatt     1260

cgcgttcctg ttgagcatac gctggatgca gtcaatattg ccgatcggta cgatgctaaa     1320

gcagtaaagg aacgtctggc gatgatgacg ccgcagaatg cgcgtatctg gtatatcagc     1380

ccgaaagagc cgcacaacaa aacggcttac tttgtcgatg cgccgtatca ggtcgataaa     1440

atcagcgcac aaactttcgc cgactggcag aaaaaagccg ccgacattgc gctctctttg     1500

ccagagctta acccttatat tcctgatgat ttctcgctga ttaagtcaga gaagaaatac     1560

gaccatccag agctgattgt tgatgagtcg aatctgcgcg tggtgtatgc gccaagccgt     1620

tattttgcca gcgagcccaa agctgatgtc agcctgattt tgcgtaatcc gaaagccatg     1680

gacagcgccc gcaatcaggt gatgtttgcg ctcaatgatt atctcgcagg gctggcgctt     1740

gatcagttaa gcaaccaggc gtcggttggt ggcataagtt tttccaccaa cgctaacaac     1800

ggccttatgg ttaatgctaa tggttacacc cagcgtctgc cgcagctgtt ccaggcattg     1860

ctcgaggggt actttagcta taccgctacg gaagatcagc ttgagcaggc gaagtcctgg     1920

tataaccaga tgatggattc cgcagaaaag ggtaaagcgt ttgagcaggc gattatgccc     1980

gcgcagatgc tctcgcaagt gccgtacttc tcgcgagatg aacggcgtaa aattttgccc     2040

tccattacgt tgaaagaggt gctggcctat cgcgacgcct taaaatcagg ggctcgacca     2100

gagtttatgg ttatcggcaa catgaccgag gcccaggcaa caacgctggc acgcgatgtg     2160

caaaaacagt tgggcgctga tggttcagag tggtgtcgaa acaaagatgt agtggtcgat     2220

aaaaaacaat ccgtcatctt tgaaaaagcc ggtaacagca ccgactccgc actggcagcg     2280

gtatttgtac cgactggcta cgatgaatac accagctcag cctatagctc tctgttgggg     2340

cagatcgtac agccgtggtt ctacaatcag ttgcgtaccg aagaacaatt gggctatgcc     2400

gtgtttgcgt ttccaatgag cgtggggcgt cagtggggca tgggcttcct tttgcaaagc     2460

aatgataaac agccttcatt cttgtgggag cgttacaagg cgtttttccc aaccgcagag     2520

gcaaaattgc gagcgatgaa gccagatgag tttgcgcaaa tccagcaggc ggtaattacc     2580

cagatgctgc aggcaccgca aacgctcggc gaagaagcat cgaagttaag taaagatttc     2640

gatcgcggca atatgcgctt cgattcgcgt gataaaatcg tggcccagat aaaactgctg     2700

acgccgcaaa aacttgctga tttcttccat caggcggtgg tcgagccgca aggcatggct     2760

attctgtcgc agatttccgg cagccagaac gggaaagccg aatatgtaca ccctgaaggc     2820

tggaaagtgt gggagaacgt cagcgcgttg cagcaaacaa tgcccctgat gagtgaaaag     2880

aatgagtga                                                             2889


<210>  5
<211>  962
<212>  PRT
<213>  Escherichia coli

<400>  5

Met Pro Arg Ser Thr Trp Phe Lys Ala Leu Leu Leu Leu Val Ala Leu 
1               5                   10                  15      


Trp Ala Pro Leu Ser Gln Ala Glu Thr Gly Trp Gln Pro Ile Gln Glu 
            20                  25                  30          


Thr Ile Arg Lys Ser Asp Lys Asp Asn Arg Gln Tyr Gln Ala Ile Arg 
        35                  40                  45              


Leu Asp Asn Gly Met Val Val Leu Leu Val Ser Asp Pro Gln Ala Val 
    50                  55                  60                  


Lys Ser Leu Ser Ala Leu Val Val Pro Val Gly Ser Leu Glu Asp Pro 
65                  70                  75                  80  


Glu Ala Tyr Gln Gly Leu Ala His Tyr Leu Glu His Met Ser Leu Met 
                85                  90                  95      


Gly Ser Lys Lys Tyr Pro Gln Ala Asp Ser Leu Ala Glu Tyr Leu Lys 
            100                 105                 110         


Met His Gly Gly Ser His Asn Ala Ser Thr Ala Pro Tyr Arg Thr Ala 
        115                 120                 125             


Phe Tyr Leu Glu Val Glu Asn Asp Ala Leu Pro Gly Ala Val Asp Arg 
    130                 135                 140                 


Leu Ala Asp Ala Ile Ala Glu Pro Leu Leu Asp Lys Lys Tyr Ala Glu 
145                 150                 155                 160 


Arg Glu Arg Asn Ala Val Asn Ala Glu Leu Thr Met Ala Arg Thr Arg 
                165                 170                 175     


Asp Gly Met Arg Met Ala Gln Val Ser Ala Glu Thr Ile Asn Pro Ala 
            180                 185                 190         


His Pro Gly Ser Lys Phe Ser Gly Gly Asn Leu Glu Thr Leu Ser Asp 
        195                 200                 205             


Lys Pro Gly Asn Pro Val Gln Gln Ala Leu Lys Asp Phe His Glu Lys 
    210                 215                 220                 


Tyr Tyr Ser Ala Asn Leu Met Lys Ala Val Ile Tyr Ser Asn Lys Pro 
225                 230                 235                 240 


Leu Pro Glu Leu Ala Lys Met Ala Ala Asp Thr Phe Gly Arg Val Pro 
                245                 250                 255     


Asn Lys Glu Ser Lys Lys Pro Glu Ile Thr Val Pro Val Val Thr Asp 
            260                 265                 270         


Ala Gln Lys Gly Ile Ile Ile His Tyr Val Pro Ala Leu Pro Arg Lys 
        275                 280                 285             


Val Leu Arg Val Glu Phe Arg Ile Asp Asn Asn Ser Ala Lys Phe Arg 
    290                 295                 300                 


Ser Lys Thr Asp Glu Leu Ile Thr Tyr Leu Ile Gly Asn Arg Ser Pro 
305                 310                 315                 320 


Gly Thr Leu Ser Asp Trp Leu Gln Lys Gln Gly Leu Val Glu Gly Ile 
                325                 330                 335     


Ser Ala Asn Ser Asp Pro Ile Val Asn Gly Asn Ser Gly Val Leu Ala 
            340                 345                 350         


Ile Ser Ala Ser Leu Thr Asp Lys Gly Leu Ala Asn Arg Asp Gln Val 
        355                 360                 365             


Val Ala Ala Ile Phe Ser Tyr Leu Asn Leu Leu Arg Glu Lys Gly Ile 
    370                 375                 380                 


Asp Lys Gln Tyr Phe Asp Glu Leu Ala Asn Val Leu Asp Ile Asp Phe 
385                 390                 395                 400 


Arg Tyr Pro Ser Ile Thr Arg Asp Met Asp Tyr Val Glu Trp Leu Ala 
                405                 410                 415     


Asp Thr Met Ile Arg Val Pro Val Glu His Thr Leu Asp Ala Val Asn 
            420                 425                 430         


Ile Ala Asp Arg Tyr Asp Ala Lys Ala Val Lys Glu Arg Leu Ala Met 
        435                 440                 445             


Met Thr Pro Gln Asn Ala Arg Ile Trp Tyr Ile Ser Pro Lys Glu Pro 
    450                 455                 460                 


His Asn Lys Thr Ala Tyr Phe Val Asp Ala Pro Tyr Gln Val Asp Lys 
465                 470                 475                 480 


Ile Ser Ala Gln Thr Phe Ala Asp Trp Gln Lys Lys Ala Ala Asp Ile 
                485                 490                 495     


Ala Leu Ser Leu Pro Glu Leu Asn Pro Tyr Ile Pro Asp Asp Phe Ser 
            500                 505                 510         


Leu Ile Lys Ser Glu Lys Lys Tyr Asp His Pro Glu Leu Ile Val Asp 
        515                 520                 525             


Glu Ser Asn Leu Arg Val Val Tyr Ala Pro Ser Arg Tyr Phe Ala Ser 
    530                 535                 540                 


Glu Pro Lys Ala Asp Val Ser Leu Ile Leu Arg Asn Pro Lys Ala Met 
545                 550                 555                 560 


Asp Ser Ala Arg Asn Gln Val Met Phe Ala Leu Asn Asp Tyr Leu Ala 
                565                 570                 575     


Gly Leu Ala Leu Asp Gln Leu Ser Asn Gln Ala Ser Val Gly Gly Ile 
            580                 585                 590         


Ser Phe Ser Thr Asn Ala Asn Asn Gly Leu Met Val Asn Ala Asn Gly 
        595                 600                 605             


Tyr Thr Gln Arg Leu Pro Gln Leu Phe Gln Ala Leu Leu Glu Gly Tyr 
    610                 615                 620                 


Phe Ser Tyr Thr Ala Thr Glu Asp Gln Leu Glu Gln Ala Lys Ser Trp 
625                 630                 635                 640 


Tyr Asn Gln Met Met Asp Ser Ala Glu Lys Gly Lys Ala Phe Glu Gln 
                645                 650                 655     


Ala Ile Met Pro Ala Gln Met Leu Ser Gln Val Pro Tyr Phe Ser Arg 
            660                 665                 670         


Asp Glu Arg Arg Lys Ile Leu Pro Ser Ile Thr Leu Lys Glu Val Leu 
        675                 680                 685             


Ala Tyr Arg Asp Ala Leu Lys Ser Gly Ala Arg Pro Glu Phe Met Val 
    690                 695                 700                 


Ile Gly Asn Met Thr Glu Ala Gln Ala Thr Thr Leu Ala Arg Asp Val 
705                 710                 715                 720 


Gln Lys Gln Leu Gly Ala Asp Gly Ser Glu Trp Cys Arg Asn Lys Asp 
                725                 730                 735     


Val Val Val Asp Lys Lys Gln Ser Val Ile Phe Glu Lys Ala Gly Asn 
            740                 745                 750         


Ser Thr Asp Ser Ala Leu Ala Ala Val Phe Val Pro Thr Gly Tyr Asp 
        755                 760                 765             


Glu Tyr Thr Ser Ser Ala Tyr Ser Ser Leu Leu Gly Gln Ile Val Gln 
    770                 775                 780                 


Pro Trp Phe Tyr Asn Gln Leu Arg Thr Glu Glu Gln Leu Gly Tyr Ala 
785                 790                 795                 800 


Val Phe Ala Phe Pro Met Ser Val Gly Arg Gln Trp Gly Met Gly Phe 
                805                 810                 815     


Leu Leu Gln Ser Asn Asp Lys Gln Pro Ser Phe Leu Trp Glu Arg Tyr 
            820                 825                 830         


Lys Ala Phe Phe Pro Thr Ala Glu Ala Lys Leu Arg Ala Met Lys Pro 
        835                 840                 845             


Asp Glu Phe Ala Gln Ile Gln Gln Ala Val Ile Thr Gln Met Leu Gln 
    850                 855                 860                 


Ala Pro Gln Thr Leu Gly Glu Glu Ala Ser Lys Leu Ser Lys Asp Phe 
865                 870                 875                 880 


Asp Arg Gly Asn Met Arg Phe Asp Ser Arg Asp Lys Ile Val Ala Gln 
                885                 890                 895     


Ile Lys Leu Leu Thr Pro Gln Lys Leu Ala Asp Phe Phe His Gln Ala 
            900                 905                 910         


Val Val Glu Pro Gln Gly Met Ala Ile Leu Ser Gln Ile Ser Gly Ser 
        915                 920                 925             


Gln Asn Gly Lys Ala Glu Tyr Val His Pro Glu Gly Trp Lys Val Trp 
    930                 935                 940                 


Glu Asn Val Ser Ala Leu Gln Gln Thr Met Pro Leu Met Ser Glu Lys 
945                 950                 955                 960 


Asn Glu 
        


<210>  6
<211>  2889
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutated knockout Protease III gene

<400>  6
attccccgca gcacctggtt caaagcatta ttgttgttag ttgccctttg ggcacattaa       60

tgtcaggcag aaacgggatg gcagccgatt caggaaacca tccgtaaaag tgataaagat      120

aaccgccagt atcaggctat acgtctggat aacggtatgg tggtcttgct ggtttctgat      180

ccgcaggcag ttaaatcgct ctcggcgctg gtggtgcccg ttgggtcgct ggaagatccc      240

gaggcgtacc aggggctggc acattacctt gaacatatga gtctgatggg gtcgaaaaag      300

tacccgcagg ctgacagtct ggccgaatat ctcaaaatgc acggcggtag tcacaatgcc      360

agcactgcgc cgtatcgcac ggctttctat ctggaagttg agaacgacgc cttgcctggt      420

gcggtagacc gcctggccga tgctattgct gaacctttgc tcgacaagaa atatgccgaa      480

cgtgagcgta atgcggtgaa cgctgaatta accatggcgc gtacgcgtga cgggatgcgc      540

atggcacagg tcagcgcaga aaccattaac ccggcacacc ccggttcaaa gttttctggt      600

ggtaacctcg aaactttaag cgacaaacct ggtaatccgg tgcagcaggc gctgaaagat      660

ttccacgaga agtactattc cgccaatttg atgaaggcgg ttatttacag taataaaccg      720

ctgccggagt tggcaaaaat ggcggcggac acctttggtc gcgtgccgaa caaagagagc      780

aaaaaaccgg aaatcaccgt gccggtagtc accgacgcgc aaaagggcat tatcattcat      840

tacgtccctg cgctgccgcg taaagtgttg cgcgttgagt ttcgcatcga taacaactca      900

gcgaagttcc gtagtaaaac cgatgaattg attacctatc tgattggcaa tcgcagccca      960

ggtacacttt ctgactggct gcaaaagcag ggattagttg agggcattag cgccaactcc     1020

gatcctatcg tcaacggcaa cagcggcgta ttagcgatct ctgcgtcttt aaccgataaa     1080

ggcctggcta atcgcgatca ggttgtggcg gcaattttta gctatctcaa tctgttacgt     1140

gaaaaaggca ttgataaaca atacttcgat gaactggcga atgtgctgga tatcgacttc     1200

cgttatccgt cgatcacccg tgatatggat tacgtcgaat ggctggcaga taccatgatt     1260

cgcgttcctg ttgagcatac gctggatgca gtcaatattg ccgatcggta cgatgctaaa     1320

gcagtaaagg aacgtctggc gatgatgacg ccgcagaatg cgcgtatctg gtatatcagc     1380

ccgaaagagc cgcacaacaa aacggcttac tttgtcgatg cgccgtatca ggtcgataaa     1440

atcagcgcac aaactttcgc cgactggcag aaaaaagccg ccgacattgc gctctctttg     1500

ccagagctta acccttatat tcctgatgat ttctcgctga ttaagtcaga gaagaaatac     1560

gaccatccag agctgattgt tgatgagtcg aatctgcgcg tggtgtatgc gccaagccgt     1620

tattttgcca gcgagcccaa agctgatgtc agcctgattt tgcgtaatcc gaaagccatg     1680

gacagcgccc gcaatcaggt gatgtttgcg ctcaatgatt atctcgcagg gctggcgctt     1740

gatcagttaa gcaaccaggc gtcggttggt ggcataagtt tttccaccaa cgctaacaac     1800

ggccttatgg ttaatgctaa tggttacacc cagcgtctgc cgcagctgtt ccaggcattg     1860

ctcgaggggt actttagcta taccgctacg gaagatcagc ttgagcaggc gaagtcctgg     1920

tataaccaga tgatggattc cgcagaaaag ggtaaagcgt ttgagcaggc gattatgccc     1980

gcgcagatgc tctcgcaagt gccgtacttc tcgcgagatg aacggcgtaa aattttgccc     2040

tccattacgt tgaaagaggt gctggcctat cgcgacgcct taaaatcagg ggctcgacca     2100

gagtttatgg ttatcggcaa catgaccgag gcccaggcaa caacgctggc acgcgatgtg     2160

caaaaacagt tgggcgctga tggttcagag tggtgtcgaa acaaagatgt agtggtcgat     2220

aaaaaacaat ccgtcatctt tgaaaaagcc ggtaacagca ccgactccgc actggcagcg     2280

gtatttgtac cgactggcta cgatgaatac accagctcag cctatagctc tctgttgggg     2340

cagatcgtac agccgtggtt ctacaatcag ttgcgtaccg aagaacaatt gggctatgcc     2400

gtgtttgcgt ttccaatgag cgtggggcgt cagtggggca tgggcttcct tttgcaaagc     2460

aatgataaac agccttcatt cttgtgggag cgttacaagg cgtttttccc aaccgcagag     2520

gcaaaattgc gagcgatgaa gccagatgag tttgcgcaaa tccagcaggc ggtaattacc     2580

cagatgctgc aggcaccgca aacgctcggc gaagaagcat cgaagttaag taaagatttc     2640

gatcgcggca atatgcgctt cgattcgcgt gataaaatcg tggcccagat aaaactgctg     2700

acgccgcaaa aacttgctga tttcttccat caggcggtgg tcgagccgca aggcatggct     2760

attctgtcgc agatttccgg cagccagaac gggaaagccg aatatgtaca ccctgaaggc     2820

tggaaagtgt gggagaacgt cagcgcgttg cagcaaacaa tgcccctgat gagtgaaaag     2880

aatgagtga                                                             2889


<210>  7
<211>  1425
<212>  DNA
<213>  Escherichia coli

<400>  7
atgaaaaaaa ccacattagc actgagtgca ctggctctga gtttaggttt ggcgttatct       60

ccgctctctg caacggcggc tgagacttct tcagcaacga cagcccagca gatgccaagc      120

cttgcaccga tgctcgaaaa ggtgatgcct tcagtggtca gcattaacgt agaaggtagc      180

acaaccgtta atacgccgcg tatgccgcgt aatttccagc agttcttcgg tgatgattct      240

ccgttctgcc aggaaggttc tccgttccag agctctccgt tctgccaggg tggccagggc      300

ggtaatggtg gcggccagca acagaaattc atggcgctgg gttccggcgt catcattgat      360

gccgataaag gctatgtcgt caccaacaac cacgttgttg ataacgcgac ggtcattaaa      420

gttcaactga gcgatggccg taagttcgac gcgaagatgg ttggcaaaga tccgcgctct      480

gatatcgcgc tgatccaaat ccagaacccg aaaaacctga ccgcaattaa gatggcggat      540

tctgatgcac tgcgcgtggg tgattacacc gtagcgattg gtaacccgtt tggtctgggc      600

gagacggtaa cttccgggat tgtctctgcg ctggggcgta gcggcctgaa tgccgaaaac      660

tacgaaaact tcatccagac cgatgcagcg atcaaccgtg gtaactccgg tggtgcgctg      720

gttaacctga acggcgaact gatcggtatc aacaccgcga tcctcgcacc ggacggcggc      780

aacatcggta tcggttttgc tatcccgagt aacatggtga aaaacctgac ctcgcagatg      840

gtggaatacg gccaggtgaa acgcggtgag ctgggtatta tggggactga gctgaactcc      900

gaactggcga aagcgatgaa agttgacgcc cagcgcggtg ctttcgtaag ccaggttctg      960

cctaattcct ccgctgcaaa agcgggcatt aaagcgggtg atgtgatcac ctcactgaac     1020

ggtaagccga tcagcagctt tgccgcactg cgtgctcagg tgggtactat gccggtaggc     1080

agcaaactga ccctgggctt actgcgcgac ggtaagcagg ttaacgtgaa cctggaactg     1140

cagcagagca gccagaatca ggttgattcc agctccatct tcaacggcat tgaaggcgct     1200

gagatgagca acaaaggcaa agatcagggc gtggtagtga acaacgtgaa aacgggcact     1260

ccggctgcgc agatcggcct gaagaaaggt gatgtgatta ttggcgcgaa ccagcaggca     1320

gtgaaaaaca tcgctgaact gcgtaaagtt ctcgacagca aaccgtctgt gctggcactc     1380

aacattcagc gcggcgacag caccatctac ctgttaatgc agtaa                     1425


<210>  8
<211>  474
<212>  PRT
<213>  Escherichia coli

<400>  8

Met Lys Lys Thr Thr Leu Ala Leu Ser Ala Leu Ala Leu Ser Leu Gly 
1               5                   10                  15      


Leu Ala Leu Ser Pro Leu Ser Ala Thr Ala Ala Glu Thr Ser Ser Ala 
            20                  25                  30          


Thr Thr Ala Gln Gln Met Pro Ser Leu Ala Pro Met Leu Glu Lys Val 
        35                  40                  45              


Met Pro Ser Val Val Ser Ile Asn Val Glu Gly Ser Thr Thr Val Asn 
    50                  55                  60                  


Thr Pro Arg Met Pro Arg Asn Phe Gln Gln Phe Phe Gly Asp Asp Ser 
65                  70                  75                  80  


Pro Phe Cys Gln Glu Gly Ser Pro Phe Gln Ser Ser Pro Phe Cys Gln 
                85                  90                  95      


Gly Gly Gln Gly Gly Asn Gly Gly Gly Gln Gln Gln Lys Phe Met Ala 
            100                 105                 110         


Leu Gly Ser Gly Val Ile Ile Asp Ala Asp Lys Gly Tyr Val Val Thr 
        115                 120                 125             


Asn Asn His Val Val Asp Asn Ala Thr Val Ile Lys Val Gln Leu Ser 
    130                 135                 140                 


Asp Gly Arg Lys Phe Asp Ala Lys Met Val Gly Lys Asp Pro Arg Ser 
145                 150                 155                 160 


Asp Ile Ala Leu Ile Gln Ile Gln Asn Pro Lys Asn Leu Thr Ala Ile 
                165                 170                 175     


Lys Met Ala Asp Ser Asp Ala Leu Arg Val Gly Asp Tyr Thr Val Ala 
            180                 185                 190         


Ile Gly Asn Pro Phe Gly Leu Gly Glu Thr Val Thr Ser Gly Ile Val 
        195                 200                 205             


Ser Ala Leu Gly Arg Ser Gly Leu Asn Ala Glu Asn Tyr Glu Asn Phe 
    210                 215                 220                 


Ile Gln Thr Asp Ala Ala Ile Asn Arg Gly Asn Ser Gly Gly Ala Leu 
225                 230                 235                 240 


Val Asn Leu Asn Gly Glu Leu Ile Gly Ile Asn Thr Ala Ile Leu Ala 
                245                 250                 255     


Pro Asp Gly Gly Asn Ile Gly Ile Gly Phe Ala Ile Pro Ser Asn Met 
            260                 265                 270         


Val Lys Asn Leu Thr Ser Gln Met Val Glu Tyr Gly Gln Val Lys Arg 
        275                 280                 285             


Gly Glu Leu Gly Ile Met Gly Thr Glu Leu Asn Ser Glu Leu Ala Lys 
    290                 295                 300                 


Ala Met Lys Val Asp Ala Gln Arg Gly Ala Phe Val Ser Gln Val Leu 
305                 310                 315                 320 


Pro Asn Ser Ser Ala Ala Lys Ala Gly Ile Lys Ala Gly Asp Val Ile 
                325                 330                 335     


Thr Ser Leu Asn Gly Lys Pro Ile Ser Ser Phe Ala Ala Leu Arg Ala 
            340                 345                 350         


Gln Val Gly Thr Met Pro Val Gly Ser Lys Leu Thr Leu Gly Leu Leu 
        355                 360                 365             


Arg Asp Gly Lys Gln Val Asn Val Asn Leu Glu Leu Gln Gln Ser Ser 
    370                 375                 380                 


Gln Asn Gln Val Asp Ser Ser Ser Ile Phe Asn Gly Ile Glu Gly Ala 
385                 390                 395                 400 


Glu Met Ser Asn Lys Gly Lys Asp Gln Gly Val Val Val Asn Asn Val 
                405                 410                 415     


Lys Thr Gly Thr Pro Ala Ala Gln Ile Gly Leu Lys Lys Gly Asp Val 
            420                 425                 430         


Ile Ile Gly Ala Asn Gln Gln Ala Val Lys Asn Ile Ala Glu Leu Arg 
        435                 440                 445             


Lys Val Leu Asp Ser Lys Pro Ser Val Leu Ala Leu Asn Ile Gln Arg 
    450                 455                 460                 


Gly Asp Ser Thr Ile Tyr Leu Leu Met Gln 
465                 470                 


<210>  9
<211>  1425
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutated DegP

<400>  9
atgaaaaaaa ccacattagc actgagtgca ctggctctga gtttaggttt ggcgttatct       60

ccgctctctg caacggcggc tgagacttct tcagcaacga cagcccagca gatgccaagc      120

cttgcaccga tgctcgaaaa ggtgatgcct tcagtggtca gcattaacgt agaaggtagc      180

acaaccgtta atacgccgcg tatgccgcgt aatttccagc agttcttcgg tgatgattct      240

ccgttctgcc aggaaggttc tccgttccag agctctccgt tctgccaggg tggccagggc      300

ggtaatggtg gcggccagca acagaaattc atggcgctgg gttccggcgt catcattgat      360

gccgataaag gctatgtcgt caccaacaac cacgttgttg ataacgcgac ggtcattaaa      420

gttcaactga gcgatggccg taagttcgac gcgaagatgg ttggcaaaga tccgcgctct      480

gatatcgcgc tgatccaaat ccagaacccg aaaaacctga ccgcaattaa gatggcggat      540

tctgatgcac tgcgcgtggg tgattacacc gtagcgattg gtaacccgtt tggtctgggc      600

gagacggtaa cttccgggat tgtctctgcg ctggggcgta gcggcctgaa tgccgaaaac      660

tacgaaaact tcatccagac cgatgcagcg attaatcgtg gtaacgccgg tggtgcgctg      720

gttaacctga acggcgaact gatcggtatc aacaccgcga tcctcgcacc ggacggcggc      780

aacatcggta tcggttttgc tatcccgagt aacatggtga aaaacctgac ctcgcagatg      840

gtggaatacg gccaggtgaa acgcggtgag ctgggtatta tggggactga gctgaactcc      900

gaactggcga aagcgatgaa agttgacgcc cagcgcggtg ctttcgtaag ccaggttctg      960

cctaattcct ccgctgcaaa agcgggcatt aaagcgggtg atgtgatcac ctcactgaac     1020

ggtaagccga tcagcagctt tgccgcactg cgtgctcagg tgggtactat gccggtaggc     1080

agcaaactga ccctgggctt actgcgcgac ggtaagcagg ttaacgtgaa cctggaactg     1140

cagcagagca gccagaatca ggttgattcc agctccatct tcaacggcat tgaaggcgct     1200

gagatgagca acaaaggcaa agatcagggc gtggtagtga acaacgtgaa aacgggcact     1260

ccggctgcgc agatcggcct gaagaaaggt gatgtgatta ttggcgcgaa ccagcaggca     1320

gtgaaaaaca tcgctgaact gcgtaaagtt ctcgacagca aaccgtctgt gctggcactc     1380

aacattcagc gcggcgacag caccatctac ctgttaatgc agtaa                     1425


<210>  10
<211>  474
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutated DegP

<400>  10

Met Lys Lys Thr Thr Leu Ala Leu Ser Ala Leu Ala Leu Ser Leu Gly 
1               5                   10                  15      


Leu Ala Leu Ser Pro Leu Ser Ala Thr Ala Ala Glu Thr Ser Ser Ala 
            20                  25                  30          


Thr Thr Ala Gln Gln Met Pro Ser Leu Ala Pro Met Leu Glu Lys Val 
        35                  40                  45              


Met Pro Ser Val Val Ser Ile Asn Val Glu Gly Ser Thr Thr Val Asn 
    50                  55                  60                  


Thr Pro Arg Met Pro Arg Asn Phe Gln Gln Phe Phe Gly Asp Asp Ser 
65                  70                  75                  80  


Pro Phe Cys Gln Glu Gly Ser Pro Phe Gln Ser Ser Pro Phe Cys Gln 
                85                  90                  95      


Gly Gly Gln Gly Gly Asn Gly Gly Gly Gln Gln Gln Lys Phe Met Ala 
            100                 105                 110         


Leu Gly Ser Gly Val Ile Ile Asp Ala Asp Lys Gly Tyr Val Val Thr 
        115                 120                 125             


Asn Asn His Val Val Asp Asn Ala Thr Val Ile Lys Val Gln Leu Ser 
    130                 135                 140                 


Asp Gly Arg Lys Phe Asp Ala Lys Met Val Gly Lys Asp Pro Arg Ser 
145                 150                 155                 160 


Asp Ile Ala Leu Ile Gln Ile Gln Asn Pro Lys Asn Leu Thr Ala Ile 
                165                 170                 175     


Lys Met Ala Asp Ser Asp Ala Leu Arg Val Gly Asp Tyr Thr Val Ala 
            180                 185                 190         


Ile Gly Asn Pro Phe Gly Leu Gly Glu Thr Val Thr Ser Gly Ile Val 
        195                 200                 205             


Ser Ala Leu Gly Arg Ser Gly Leu Asn Ala Glu Asn Tyr Glu Asn Phe 
    210                 215                 220                 


Ile Gln Thr Asp Ala Ala Ile Asn Arg Gly Asn Ala Gly Gly Ala Leu 
225                 230                 235                 240 


Val Asn Leu Asn Gly Glu Leu Ile Gly Ile Asn Thr Ala Ile Leu Ala 
                245                 250                 255     


Pro Asp Gly Gly Asn Ile Gly Ile Gly Phe Ala Ile Pro Ser Asn Met 
            260                 265                 270         


Val Lys Asn Leu Thr Ser Gln Met Val Glu Tyr Gly Gln Val Lys Arg 
        275                 280                 285             


Gly Glu Leu Gly Ile Met Gly Thr Glu Leu Asn Ser Glu Leu Ala Lys 
    290                 295                 300                 


Ala Met Lys Val Asp Ala Gln Arg Gly Ala Phe Val Ser Gln Val Leu 
305                 310                 315                 320 


Pro Asn Ser Ser Ala Ala Lys Ala Gly Ile Lys Ala Gly Asp Val Ile 
                325                 330                 335     


Thr Ser Leu Asn Gly Lys Pro Ile Ser Ser Phe Ala Ala Leu Arg Ala 
            340                 345                 350         


Gln Val Gly Thr Met Pro Val Gly Ser Lys Leu Thr Leu Gly Leu Leu 
        355                 360                 365             


Arg Asp Gly Lys Gln Val Asn Val Asn Leu Glu Leu Gln Gln Ser Ser 
    370                 375                 380                 


Gln Asn Gln Val Asp Ser Ser Ser Ile Phe Asn Gly Ile Glu Gly Ala 
385                 390                 395                 400 


Glu Met Ser Asn Lys Gly Lys Asp Gln Gly Val Val Val Asn Asn Val 
                405                 410                 415     


Lys Thr Gly Thr Pro Ala Ala Gln Ile Gly Leu Lys Lys Gly Asp Val 
            420                 425                 430         


Ile Ile Gly Ala Asn Gln Gln Ala Val Lys Asn Ile Ala Glu Leu Arg 
        435                 440                 445             


Lys Val Leu Asp Ser Lys Pro Ser Val Leu Ala Leu Asn Ile Gln Arg 
    450                 455                 460                 


Gly Asp Ser Thr Ile Tyr Leu Leu Met Gln 
465                 470                 


<210>  11
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5' oligonucleotide primer for the region of the mutated DegP gene
       comprising the Ase I restriction site

<400>  11
ctgcctgcga ttttcgccgg aacg                                              24


<210>  12
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' oligonucleotide primer for the region of the mutated DegP gene
       comprising the Ase I restriction site

<400>  12
cgcatggtac gtgccacgat atcc                                              24


<210>  13
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  the 5' oligonucleotide primer for the region of the mutated Tsp 
       gene comprising the Ase I restriction site

<400>  13
gggaaatgaa cctgagcaaa acgc                                              24


<210>  14
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' oligonucleotide primer for the region of the mutated Protease 
       III gene comprising the Ase I restriction site

<400>  14
gggaaaggcg gcggaaccgc ctag                                              24


<210>  15
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5' oligonucleotide primer for the region of the mutated Protease 
       III gene comprising the Ase I restriction site

<400>  15
ctactgtgcc agcggtggta atgg                                              24


<210>  16
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' oligonucleotide primer for the region of the mutated Tsp gene 
       comprising the Ase I restriction site

<400>  16
gcatcataat tttcttttta cctc                                              24


<210>  17
<211>  564
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  wild-type spr gene

<400>  17
atggtcaaat ctcaaccgat tttgagatat atcttgcgcg ggattcccgc gattgcagta       60

gcggttctgc tttctgcatg tagtgcaaat aacaccgcaa agaatatgca tcctgagaca      120

cgtgcagtgg gtagtgaaac atcatcactg caagcttctc aggatgaatt tgaaaacctg      180

gttcgtaatg tcgacgtaaa atcgcgaatt atggatcagt atgctgactg gaaaggcgta      240

cgttatcgtc tgggcggcag cactaaaaaa ggtatcgatt gttctggttt cgtacagcgt      300

acattccgtg agcaatttgg cttagaactt ccgcgttcga cttacgaaca gcaggaaatg      360

ggtaaatctg tttcccgcag taatttgcgt acgggtgatt tagttctgtt ccgtgccggt      420

tcaacgggac gccatgtcgg tatttatatc ggcaacaatc agtttgtcca tgcttccacc      480

agcagtggtg ttattatttc cagcatgaat gaaccgtact ggaagaagcg ttacaacgaa      540

gcacgccggg ttctcagccg cagc                                             564


<210>  18
<211>  188
<212>  PRT
<213>  Escherichia coli

<400>  18

Met Val Lys Ser Gln Pro Ile Leu Arg Tyr Ile Leu Arg Gly Ile Pro 
1               5                   10                  15      


Ala Ile Ala Val Ala Val Leu Leu Ser Ala Cys Ser Ala Asn Asn Thr 
            20                  25                  30          


Ala Lys Asn Met His Pro Glu Thr Arg Ala Val Gly Ser Glu Thr Ser 
        35                  40                  45              


Ser Leu Gln Ala Ser Gln Asp Glu Phe Glu Asn Leu Val Arg Asn Val 
    50                  55                  60                  


Asp Val Lys Ser Arg Ile Met Asp Gln Tyr Ala Asp Trp Lys Gly Val 
65                  70                  75                  80  


Arg Tyr Arg Leu Gly Gly Ser Thr Lys Lys Gly Ile Asp Cys Ser Gly 
                85                  90                  95      


Phe Val Gln Arg Thr Phe Arg Glu Gln Phe Gly Leu Glu Leu Pro Arg 
            100                 105                 110         


Ser Thr Tyr Glu Gln Gln Glu Met Gly Lys Ser Val Ser Arg Ser Asn 
        115                 120                 125             


Leu Arg Thr Gly Asp Leu Val Leu Phe Arg Ala Gly Ser Thr Gly Arg 
    130                 135                 140                 


His Val Gly Ile Tyr Ile Gly Asn Asn Gln Phe Val His Ala Ser Thr 
145                 150                 155                 160 


Ser Ser Gly Val Ile Ile Ser Ser Met Asn Glu Pro Tyr Trp Lys Lys 
                165                 170                 175     


Arg Tyr Asn Glu Ala Arg Arg Val Leu Ser Arg Ser 
            180                 185             


<210>  19
<211>  162
<212>  PRT
<213>  Escherichia coli

<400>  19

Cys Ser Ala Asn Asn Thr Ala Lys Asn Met His Pro Glu Thr Arg Ala 
1               5                   10                  15      


Val Gly Ser Glu Thr Ser Ser Leu Gln Ala Ser Gln Asp Glu Phe Glu 
            20                  25                  30          


Asn Leu Val Arg Asn Val Asp Val Lys Ser Arg Ile Met Asp Gln Tyr 
        35                  40                  45              


Ala Asp Trp Lys Gly Val Arg Tyr Arg Leu Gly Gly Ser Thr Lys Lys 
    50                  55                  60                  


Gly Ile Asp Cys Ser Gly Phe Val Gln Arg Thr Phe Arg Glu Gln Phe 
65                  70                  75                  80  


Gly Leu Glu Leu Pro Arg Ser Thr Tyr Glu Gln Gln Glu Met Gly Lys 
                85                  90                  95      


Ser Val Ser Arg Ser Asn Leu Arg Thr Gly Asp Leu Val Leu Phe Arg 
            100                 105                 110         


Ala Gly Ser Thr Gly Arg His Val Gly Ile Tyr Ile Gly Asn Asn Gln 
        115                 120                 125             


Phe Val His Ala Ser Thr Ser Ser Gly Val Ile Ile Ser Ser Met Asn 
    130                 135                 140                 


Glu Pro Tyr Trp Lys Lys Arg Tyr Asn Glu Ala Arg Arg Val Leu Ser 
145                 150                 155                 160 


Arg Ser 
        


<210>  20
<211>  954
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence of a mutated OmpT sequence comprising D210A 
       and H212A mutations

<400>  20
atgcgggcga aacttctggg aatagtcctg acaaccccta ttgcgatcag ctcttttgct       60

tctaccgaga ctttatcgtt tactcctgac aacataaatg cggacattag tcttggaact      120

ctgagcggaa aaacaaaaga gcgtgtttat ctagccgaag aaggaggccg aaaagtcagt      180

caactcgact ggaaattcaa taacgctgca attattaaag gtgcaattaa ttgggatttg      240

atgccccaga tatctatcgg ggctgctggc tggacaactc tcggcagccg aggtggcaat      300

atggtcgatc aggactggat ggattccagt aaccccggaa cctggacgga tgaaagtaga      360

caccctgata cacaactcaa ttatgccaac gaatttgatc tgaatatcaa aggctggctc      420

ctcaacgaac ccaattaccg cctgggactc atggccggat atcaggaaag ccgttatagc      480

tttacagcca gaggtggttc ctatatctac agttctgagg agggattcag agatgatatc      540

ggctccttcc cgaatggaga aagagcaatc ggctacaaac aacgttttaa aatgccctac      600

attggcttga ctggaagtta tcgttatgaa gattttgaac tcggtggcac atttaaatac      660

agcggctggg tggaatcatc tgataacgct gaagcttatg acccgggaaa aagaatcact      720

tatcgcagta aggtcaaaga ccaaaattac tattctgttg cagtcaatgc aggttattac      780

gtcacaccta acgcaaaagt ttatgttgaa ggcgcatgga atcgggttac gaataaaaaa      840

ggtaatactt cactttatga tcacaataat aacacttcag actacagcaa aaatggagca      900

ggtatagaaa actataactt catcactact gctggtctta agtacacatt ttaa            954


<210>  21
<211>  317
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutated OmpT sequence comprising D210A and H212A mutations

<400>  21

Met Arg Ala Lys Leu Leu Gly Ile Val Leu Thr Thr Pro Ile Ala Ile 
1               5                   10                  15      


Ser Ser Phe Ala Ser Thr Glu Thr Leu Ser Phe Thr Pro Asp Asn Ile 
            20                  25                  30          


Asn Ala Asp Ile Ser Leu Gly Thr Leu Ser Gly Lys Thr Lys Glu Arg 
        35                  40                  45              


Val Tyr Leu Ala Glu Glu Gly Gly Arg Lys Val Ser Gln Leu Asp Trp 
    50                  55                  60                  


Lys Phe Asn Asn Ala Ala Ile Ile Lys Gly Ala Ile Asn Trp Asp Leu 
65                  70                  75                  80  


Met Pro Gln Ile Ser Ile Gly Ala Ala Gly Trp Thr Thr Leu Gly Ser 
                85                  90                  95      


Arg Gly Gly Asn Met Val Asp Gln Asp Trp Met Asp Ser Ser Asn Pro 
            100                 105                 110         


Gly Thr Trp Thr Asp Glu Ser Arg His Pro Asp Thr Gln Leu Asn Tyr 
        115                 120                 125             


Ala Asn Glu Phe Asp Leu Asn Ile Lys Gly Trp Leu Leu Asn Glu Pro 
    130                 135                 140                 


Asn Tyr Arg Leu Gly Leu Met Ala Gly Tyr Gln Glu Ser Arg Tyr Ser 
145                 150                 155                 160 


Phe Thr Ala Arg Gly Gly Ser Tyr Ile Tyr Ser Ser Glu Glu Gly Phe 
                165                 170                 175     


Arg Asp Asp Ile Gly Ser Phe Pro Asn Gly Glu Arg Ala Ile Gly Tyr 
            180                 185                 190         


Lys Gln Arg Phe Lys Met Pro Tyr Ile Gly Leu Thr Gly Ser Tyr Arg 
        195                 200                 205             


Tyr Glu Asp Phe Glu Leu Gly Gly Thr Phe Lys Tyr Ser Gly Trp Val 
    210                 215                 220                 


Glu Ser Ser Asp Asn Ala Glu Ala Tyr Asp Pro Gly Lys Arg Ile Thr 
225                 230                 235                 240 


Tyr Arg Ser Lys Val Lys Asp Gln Asn Tyr Tyr Ser Val Ala Val Asn 
                245                 250                 255     


Ala Gly Tyr Tyr Val Thr Pro Asn Ala Lys Val Tyr Val Glu Gly Ala 
            260                 265                 270         


Trp Asn Arg Val Thr Asn Lys Lys Gly Asn Thr Ser Leu Tyr Asp His 
        275                 280                 285             


Asn Asn Asn Thr Ser Asp Tyr Ser Lys Asn Gly Ala Gly Ile Glu Asn 
    290                 295                 300                 


Tyr Asn Phe Ile Thr Thr Ala Gly Leu Lys Tyr Thr Phe 
305                 310                 315         


<210>  22
<211>  954
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutated knockout OmpT sequence

<400>  22
attcgggcga aacttctggg aatagtcctg acaaccccta ttgcgatcag ctcttttgct       60

tctaccgaga ctttatcgtt tactcctgac aacataaatg cggacattag tcttggaact      120

ctgagcggaa aaacaaaaga gcgtgtttat ctagccgaag aaggaggccg aaaagtcagt      180

caactcgact ggaaattcaa taacgctgca attattaaag gtgcaattaa ttgggatttg      240

atgccccaga tatctatcgg ggctgctggc tggacaactc tcggcagccg aggtggcaat      300

atggtcgatc aggactggat ggattccagt aaccccggaa cctggacgga tgaaagtaga      360

caccctgata cacaactcaa ttatgccaac gaatttgatc tgaatatcaa aggctggctc      420

ctcaacgaac ccaattaccg cctgggactc atggccggat atcaggaaag ccgttatagc      480

tttacagcca gaggtggttc ctatatctac agttctgagg agggattcag agatgatatc      540

ggctccttcc cgaatggaga aagagcaatc ggctacaaac aacgttttaa aatgccctac      600

attggcttga ctggaagtta tcgttatgaa gattttgaac tcggtggcac atttaaatac      660

agcggctggg tggaatcatc tgataacgat gaacactatg acccgggaaa aagaatcact      720

tatcgcagta aggtcaaaga ccaaaattac tattctgttg cagtcaatgc aggttattac      780

gtcacaccta acgcaaaagt ttatgttgaa ggcgcatgga atcgggttac gaataaaaaa      840

ggtaatactt cactttatga tcacaataat aacacttcag actacagcaa aaatggagca      900

ggtatagaaa actataactt catcactact gctggtctta agtacacatt ttaa            954


<210>  23
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  OmpA oligonucleotide adapter

<400>  23
cgattgaatg gagaacttga attcgggcga aacttctggg aatag                       45


<210>  24
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide cassette encoding intergenic sequence 1 (IGS1) 
       for E. coli Fab expression

<400>  24
aagttttaat agaggagagt gttaatgaag aag                                    33


<210>  25
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  the oligonucleotide cassette encoding intergenic sequence 2 
       (IGS2) for E. coli Fab expression.

<400>  25
aagttttaat agaggggagt gttaaaatga agaag                                  35


<210>  26
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide cassette encoding intergenic sequence 3 (IGS3) 
       for E. coli Fab expression

<400>  26
aagctttaat agaggagagt gttgaggagg aaaaaaaaat gaagaaa                     47


<210>  27
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide cassette encoding intergenic sequence 4 (IGS4) 
       for E. coli Fab expression

<400>  27
aagctttaat agaggagagt gttgacgagg attatataat gaagaaa                     47


<210>  28
<211>  810
<212>  DNA
<213>  Escherichia coli

<400>  28
atgaaatcac tgtttaaagt aacgctgctg gcgaccacaa tggccgttgc cctgcatgca       60

ccaatcactt ttgctgctga agctgcaaaa cctgctacag ctgctgacag caaagcagcg      120

ttcaaaaatg acgatcagaa atcagcttat gcactgggtg cctcgctggg tcgttacatg      180

gaaaactctc taaaagaaca agaaaaactg ggcatcaaac tggataaaga tcagctgatc      240

gctggtgttc aggatgcatt tgctgataag agcaaactct ccgaccaaga gatcgaacag      300

actctacaag cattcgaagc tcgcgtgaag tcttctgctc aggcgaagat ggaaaaagac      360

gcggctgata acgaagcaaa aggtaaagag taccgcgaga aatttgccaa agagaaaggt      420

gtgaaaacct cttcaactgg tctggtttat caggtagtag aagccggtaa aggcgaagca      480

ccgaaagaca gcgatactgt tgtagtgaac tacaaaggta cgctgatcga cggtaaagag      540

ttcgacaact cttacacccg tggtgaaccg ctttctttcc gtctggacgg tgttatcccg      600

ggttggacag aaggtctgaa gaacatcaag aaaggcggta agatcaaact ggttattcca      660

ccagaactgg cttacggcaa agcgggtgtt ccggggatcc caccgaattc taccctggtg      720

tttgacgtag agctgctgga tgtgaaacca gcgccgaagg ctgatgcaaa gccggaagct      780

gatgcgaaag ccgcagattc tgctaaaaaa                                       810


<210>  29
<211>  270
<212>  PRT
<213>  Escherichia coli

<400>  29

Met Lys Ser Leu Phe Lys Val Thr Leu Leu Ala Thr Thr Met Ala Val 
1               5                   10                  15      


Ala Leu His Ala Pro Ile Thr Phe Ala Ala Glu Ala Ala Lys Pro Ala 
            20                  25                  30          


Thr Ala Ala Asp Ser Lys Ala Ala Phe Lys Asn Asp Asp Gln Lys Ser 
        35                  40                  45              


Ala Tyr Ala Leu Gly Ala Ser Leu Gly Arg Tyr Met Glu Asn Ser Leu 
    50                  55                  60                  


Lys Glu Gln Glu Lys Leu Gly Ile Lys Leu Asp Lys Asp Gln Leu Ile 
65                  70                  75                  80  


Ala Gly Val Gln Asp Ala Phe Ala Asp Lys Ser Lys Leu Ser Asp Gln 
                85                  90                  95      


Glu Ile Glu Gln Thr Leu Gln Ala Phe Glu Ala Arg Val Lys Ser Ser 
            100                 105                 110         


Ala Gln Ala Lys Met Glu Lys Asp Ala Ala Asp Asn Glu Ala Lys Gly 
        115                 120                 125             


Lys Glu Tyr Arg Glu Lys Phe Ala Lys Glu Lys Gly Val Lys Thr Ser 
    130                 135                 140                 


Ser Thr Gly Leu Val Tyr Gln Val Val Glu Ala Gly Lys Gly Glu Ala 
145                 150                 155                 160 


Pro Lys Asp Ser Asp Thr Val Val Val Asn Tyr Lys Gly Thr Leu Ile 
                165                 170                 175     


Asp Gly Lys Glu Phe Asp Asn Ser Tyr Thr Arg Gly Glu Pro Leu Ser 
            180                 185                 190         


Phe Arg Leu Asp Gly Val Ile Pro Gly Trp Thr Glu Gly Leu Lys Asn 
        195                 200                 205             


Ile Lys Lys Gly Gly Lys Ile Lys Leu Val Ile Pro Pro Glu Leu Ala 
    210                 215                 220                 


Tyr Gly Lys Ala Gly Val Pro Gly Ile Pro Pro Asn Ser Thr Leu Val 
225                 230                 235                 240 


Phe Asp Val Glu Leu Leu Asp Val Lys Pro Ala Pro Lys Ala Asp Ala 
                245                 250                 255     


Lys Pro Glu Ala Asp Ala Lys Ala Ala Asp Ser Ala Lys Lys 
            260                 265                 270 


<210>  30
<211>  828
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  the FkpA tagged gene

<400>  30
atgaaatcac tgtttaaagt aacgctgctg gcgaccacaa tggccgttgc cctgcacgca       60

ccaatcactt ttgctgctga agctgcaaaa cctgctactg ctgctgacag caaagcagcg      120

ttcaaaaatg acgatcagaa atcagcttat gcactgggtg cctcgctggg tcgttacatg      180

gaaaactctc taaaagaaca agaaaaactg ggcatcaaac tggataaaga tcaactgatc      240

gctggtgttc aggatgcatt tgctgataag agcaaactct ccgaccaaga gatcgaacag      300

actctacaag catttgaagc tcgcgtgaag tcttctgctc aggcgaagat ggaaaaagac      360

gcggctgata acgaagcaaa aggtaaagag taccgcgaga aatttgccaa agagaaaggt      420

gtgaaaacct cttcaactgg tctggtttat caggtagtag aagccggtaa aggcgaagca      480

ccgaaagaca gcgatactgt tgtagtgaac tacaaaggta cgctgatcga cggtaaagag      540

ttcgacaact cttacacccg tggtgaaccg ctttctttcc gtctggacgg tgttatcccg      600

ggttggacag aaggtctgaa gaacatcaag aaaggcggta agataaaact ggttattcca      660

ccagaactgg cttacggcaa agcgggtgtt ccggggattc caccaaattc taccctggtg      720

tttgacgtag agctgctgga tgtgaaacca gcgccgaagg ctgatgcaaa gccggaagct      780

gatgcgaaag ccgcagattc tgctaaaaaa caccatcacc atcaccac                   828


<210>  31
<211>  276
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FkpA his tagged gene

<400>  31

Met Lys Ser Leu Phe Lys Val Thr Leu Leu Ala Thr Thr Met Ala Val 
1               5                   10                  15      


Ala Leu His Ala Pro Ile Thr Phe Ala Ala Glu Ala Ala Lys Pro Ala 
            20                  25                  30          


Thr Ala Ala Asp Ser Lys Ala Ala Phe Lys Asn Asp Asp Gln Lys Ser 
        35                  40                  45              


Ala Tyr Ala Leu Gly Ala Ser Leu Gly Arg Tyr Met Glu Asn Ser Leu 
    50                  55                  60                  


Lys Glu Gln Glu Lys Leu Gly Ile Lys Leu Asp Lys Asp Gln Leu Ile 
65                  70                  75                  80  


Ala Gly Val Gln Asp Ala Phe Ala Asp Lys Ser Lys Leu Ser Asp Gln 
                85                  90                  95      


Glu Ile Glu Gln Thr Leu Gln Ala Phe Glu Ala Arg Val Lys Ser Ser 
            100                 105                 110         


Ala Gln Ala Lys Met Glu Lys Asp Ala Ala Asp Asn Glu Ala Lys Gly 
        115                 120                 125             


Lys Glu Tyr Arg Glu Lys Phe Ala Lys Glu Lys Gly Val Lys Thr Ser 
    130                 135                 140                 


Ser Thr Gly Leu Val Tyr Gln Val Val Glu Ala Gly Lys Gly Glu Ala 
145                 150                 155                 160 


Pro Lys Asp Ser Asp Thr Val Val Val Asn Tyr Lys Gly Thr Leu Ile 
                165                 170                 175     


Asp Gly Lys Glu Phe Asp Asn Ser Tyr Thr Arg Gly Glu Pro Leu Ser 
            180                 185                 190         


Phe Arg Leu Asp Gly Val Ile Pro Gly Trp Thr Glu Gly Leu Lys Asn 
        195                 200                 205             


Ile Lys Lys Gly Gly Lys Ile Lys Leu Val Ile Pro Pro Glu Leu Ala 
    210                 215                 220                 


Tyr Gly Lys Ala Gly Val Pro Gly Ile Pro Pro Asn Ser Thr Leu Val 
225                 230                 235                 240 


Phe Asp Val Glu Leu Leu Asp Val Lys Pro Ala Pro Lys Ala Asp Ala 
                245                 250                 255     


Lys Pro Glu Ala Asp Ala Lys Ala Ala Asp Ser Ala Lys Lys His His 
            260                 265                 270         


His His His His 
        275     


<210>  32
<211>  486
<212>  DNA
<213>  Escherichia coli

<400>  32
gtgaaaaagt ggttattagc tgcaggtctc ggtttagcac tggcaacttc tgctcaggcg       60

gctgacaaaa ttgcaatcgt caacatgggc agcctgttcc agcaggtagc gcagaaaacc      120

ggtgtttcta acacgctgga aaatgagttc aaaggccgtg ccagcgaact gcagcgtatg      180

gaaaccgatc tgcaggctaa aatgaaaaag ctgcagtcca tgaaagcggg cagcgatcgc      240

actaagctgg aaaaagacgt gatggctcag cgccagactt ttgctcagaa agcgcaggct      300

tttgagcagg atcgcgcacg tcgttccaac gaagaacgcg gcaaactggt tactcgtatc      360

cagactgctg tgaaatccgt tgccaacagc caggatatcg atctggttgt tgatgcaaac      420

gccgttgctt acaacagcag cgatgtaaaa gacatcactg ccgacgtact gaaacaggtt      480

aaataa                                                                 486


<210>  33
<211>  161
<212>  PRT
<213>  Escherichia coli

<400>  33

Met Lys Lys Trp Leu Leu Ala Ala Gly Leu Gly Leu Ala Leu Ala Thr 
1               5                   10                  15      


Ser Ala Gln Ala Ala Asp Lys Ile Ala Ile Val Asn Met Gly Ser Leu 
            20                  25                  30          


Phe Gln Gln Val Ala Gln Lys Thr Gly Val Ser Asn Thr Leu Glu Asn 
        35                  40                  45              


Glu Phe Lys Gly Arg Ala Ser Glu Leu Gln Arg Met Glu Thr Asp Leu 
    50                  55                  60                  


Gln Ala Lys Met Lys Lys Leu Gln Ser Met Lys Ala Gly Ser Asp Arg 
65                  70                  75                  80  


Thr Lys Leu Glu Lys Asp Val Met Ala Gln Arg Gln Thr Phe Ala Gln 
                85                  90                  95      


Lys Ala Gln Ala Phe Glu Gln Asp Arg Ala Arg Arg Ser Asn Glu Glu 
            100                 105                 110         


Arg Gly Lys Leu Val Thr Arg Ile Gln Thr Ala Val Lys Ser Val Ala 
        115                 120                 125             


Asn Ser Gln Asp Ile Asp Leu Val Val Asp Ala Asn Ala Val Ala Tyr 
    130                 135                 140                 


Asn Ser Ser Asp Val Lys Asp Ile Thr Ala Asp Val Leu Lys Gln Val 
145                 150                 155                 160 


Lys 
    


<210>  34
<211>  501
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  skp his tagged gene

<400>  34
atgaaaaagt ggttattagc cgcaggtctc ggtttagcac tggcaacttc tgctcaggcg       60

gctgacaaaa ttgcaatcgt caacatgggc agcctgttcc agcaggtagc gcagaaaacc      120

ggtgtttcta acacgctgga aaatgagttc aaaggccgtg ccagcgaact ccagcgtatg      180

gaaaccgatc tccaggctaa aatgaaaaag ctgcaatcca tgaaagcggg cagcgatcgc      240

actaagctgg aaaaagacgt gatggctcag cgccagactt ttgctcagaa agcgcaggct      300

tttgagcagg atcgcgcacg tcgttccaac gaagaacgcg gcaaactggt tactcgtatc      360

cagactgctg tgaaatccgt tgccaacagc caggaaatcg atctggttgt tgatgcaaac      420

gccgttgctt acaacagcag cgatgtaaaa gacatcactg ccgacgtact gaaacaggtt      480

aaacaccatc accatcacca c                                                501


<210>  35
<211>  167
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  skp his tagged gene

<400>  35

Met Lys Lys Trp Leu Leu Ala Ala Gly Leu Gly Leu Ala Leu Ala Thr 
1               5                   10                  15      


Ser Ala Gln Ala Ala Asp Lys Ile Ala Ile Val Asn Met Gly Ser Leu 
            20                  25                  30          


Phe Gln Gln Val Ala Gln Lys Thr Gly Val Ser Asn Thr Leu Glu Asn 
        35                  40                  45              


Glu Phe Lys Gly Arg Ala Ser Glu Leu Gln Arg Met Glu Thr Asp Leu 
    50                  55                  60                  


Gln Ala Lys Met Lys Lys Leu Gln Ser Met Lys Ala Gly Ser Asp Arg 
65                  70                  75                  80  


Thr Lys Leu Glu Lys Asp Val Met Ala Gln Arg Gln Thr Phe Ala Gln 
                85                  90                  95      


Lys Ala Gln Ala Phe Glu Gln Asp Arg Ala Arg Arg Ser Asn Glu Glu 
            100                 105                 110         


Arg Gly Lys Leu Val Thr Arg Ile Gln Thr Ala Val Lys Ser Val Ala 
        115                 120                 125             


Asn Ser Gln Glu Ile Asp Leu Val Val Asp Ala Asn Ala Val Ala Tyr 
    130                 135                 140                 


Asn Ser Ser Asp Val Lys Asp Ile Thr Ala Asp Val Leu Lys Gln Val 
145                 150                 155                 160 


Lys His His His His His His 
                165         


<210>  36
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CA170 1519 Ab CDRH1

<400>  36

Gly Phe Thr Phe Ser Asn Tyr Gly Met Val 
1               5                   10  


<210>  37
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CA170_1519 Ab CDRH2

<400>  37

Tyr Ile Asp Ser Asp Gly Asp Asn Thr Tyr Tyr Arg Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  38
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CA170_1519 Ab CDRH3

<400>  38

Gly Ile Val Arg Pro Phe Leu Tyr 
1               5               


<210>  39
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CA170_1519 Ab CDRL1

<400>  39

Lys Ser Ser Gln Ser Leu Val Gly Ala Ser Gly Lys Thr Tyr Leu Tyr 
1               5                   10                  15      


<210>  40
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CA170_1519 Ab CDRL2

<400>  40

Leu Val Ser Thr Leu Asp Ser 
1               5           


<210>  41
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CA170_1519 Ab CDRL3

<400>  41

Leu Gln Gly Thr His Phe Pro His Thr 
1               5                   


<210>  42
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Rat Ab 1519 VL region

<400>  42

Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Ser Val Ala Leu Gly 
1               5                   10                  15      


Gln Pro Ala Ser Ile Ser Cys Lys Ser Ser Gln Ser Leu Val Gly Ala 
            20                  25                  30          


Ser Gly Lys Thr Tyr Leu Tyr Trp Leu Phe Gln Arg Ser Gly Gln Ser 
        35                  40                  45              


Pro Lys Arg Leu Ile Tyr Leu Val Ser Thr Leu Asp Ser Gly Ile Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ala Glu Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Arg Arg Val Glu Ala Asp Asp Leu Gly Val Tyr Tyr Cys Leu Gln Gly 
                85                  90                  95      


Thr His Phe Pro His Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
            100                 105                 110         


<210>  43
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Rat Ab 1519 VL region

<400>  43
gatgttgtga tgacccagac tccactgtct ttgtcggttg cccttggaca accagcctcc       60

atctcttgca agtcaagtca gagcctcgta ggtgctagtg gaaagacata tttgtattgg      120

ttatttcaga ggtccggcca gtctccaaag cgactaatct atctggtgtc cacactggac      180

tctggaattc ctgataggtt cagtggcagt ggagcagaga cagattttac tcttaaaatc      240

cgcagagtgg aagccgatga tttgggagtt tattactgct tgcaaggtac acattttcct      300

cacacgtttg gagctgggac caagctggaa ttgaaa                                336


<210>  44
<211>  132
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Rat Ab 1519 VL region

<400>  44

Met Met Ser Pro Ala Gln Phe Leu Phe Leu Leu Met Leu Trp Ile Gln 
1               5                   10                  15      


Gly Thr Ser Gly Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Ser 
            20                  25                  30          


Val Ala Leu Gly Gln Pro Ala Ser Ile Ser Cys Lys Ser Ser Gln Ser 
        35                  40                  45              


Leu Val Gly Ala Ser Gly Lys Thr Tyr Leu Tyr Trp Leu Phe Gln Arg 
    50                  55                  60                  


Ser Gly Gln Ser Pro Lys Arg Leu Ile Tyr Leu Val Ser Thr Leu Asp 
65                  70                  75                  80  


Ser Gly Ile Pro Asp Arg Phe Ser Gly Ser Gly Ala Glu Thr Asp Phe 
                85                  90                  95      


Thr Leu Lys Ile Arg Arg Val Glu Ala Asp Asp Leu Gly Val Tyr Tyr 
            100                 105                 110         


Cys Leu Gln Gly Thr His Phe Pro His Thr Phe Gly Ala Gly Thr Lys 
        115                 120                 125             


Leu Glu Leu Lys 
    130         


<210>  45
<211>  396
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Rat Ab 1519 VL region

<400>  45
atgatgagtc ctgcccagtt cctgtttctg ctgatgctct ggattcaggg aaccagtggt       60

gatgttgtga tgacccagac tccactgtct ttgtcggttg cccttggaca accagcctcc      120

atctcttgca agtcaagtca gagcctcgta ggtgctagtg gaaagacata tttgtattgg      180

ttatttcaga ggtccggcca gtctccaaag cgactaatct atctggtgtc cacactggac      240

tctggaattc ctgataggtt cagtggcagt ggagcagaga cagattttac tcttaaaatc      300

cgcagagtgg aagccgatga tttgggagtt tattactgct tgcaaggtac acattttcct      360

cacacgtttg gagctgggac caagctggaa ttgaaa                                396


<210>  46
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Rat Ab 1519 VH region

<400>  46

Glu Val Pro Leu Val Glu Ser Gly Gly Gly Ser Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Met Lys Leu Ser Cys Val Val Ser Gly Phe Thr Phe Ser Asn Tyr 
            20                  25                  30          


Gly Met Val Trp Val Arg Gln Ala Pro Lys Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Tyr Ile Asp Ser Asp Gly Asp Asn Thr Tyr Tyr Arg Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asn Asn Ala Lys Ser Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asp Ser Leu Arg Ser Glu Asp Thr Ala Thr Tyr Tyr Cys 
                85                  90                  95      


Thr Thr Gly Ile Val Arg Pro Phe Leu Tyr Trp Gly Gln Gly Thr Thr 
            100                 105                 110         


Val Thr Val Ser 
        115     


<210>  47
<211>  348
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Rat Ab 1519 VH region

<400>  47
gaggtgccgc tggtggagtc tgggggcggc tcagtgcagc ctgggaggtc catgaaactc       60

tcctgtgtag tctcaggatt cactttcagt aattatggca tggtctgggt ccgccaggct      120

ccaaagaagg gtctggagtg ggtcgcatat attgattctg atggtgataa tacttactac      180

cgagattccg tgaagggccg attcactatc tccagaaata atgcaaaaag caccctatat      240

ttgcaaatgg acagtctgag gtctgaggac acggccactt attactgtac aacagggatt      300

gtccggccct ttctctattg gggccaagga accacggtca ccgtctcg                   348


<210>  48
<211>  135
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Rat Ab 1519 VH region

<400>  48

Met Asp Ile Ser Leu Ser Leu Ala Phe Leu Val Leu Phe Ile Lys Gly 
1               5                   10                  15      


Val Arg Cys Glu Val Pro Leu Val Glu Ser Gly Gly Gly Ser Val Gln 
            20                  25                  30          


Pro Gly Arg Ser Met Lys Leu Ser Cys Val Val Ser Gly Phe Thr Phe 
        35                  40                  45              


Ser Asn Tyr Gly Met Val Trp Val Arg Gln Ala Pro Lys Lys Gly Leu 
    50                  55                  60                  


Glu Trp Val Ala Tyr Ile Asp Ser Asp Gly Asp Asn Thr Tyr Tyr Arg 
65                  70                  75                  80  


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asn Asn Ala Lys Ser 
                85                  90                  95      


Thr Leu Tyr Leu Gln Met Asp Ser Leu Arg Ser Glu Asp Thr Ala Thr 
            100                 105                 110         


Tyr Tyr Cys Thr Thr Gly Ile Val Arg Pro Phe Leu Tyr Trp Gly Gln 
        115                 120                 125             


Gly Thr Thr Val Thr Val Ser 
    130                 135 


<210>  49
<211>  405
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Rat Ab 1519 VH region

<400>  49
atggacatca gtctcagctt ggctttcctt gtccttttca taaaaggtgt ccggtgtgag       60

gtgccgctgg tggagtctgg gggcggctca gtgcagcctg ggaggtccat gaaactctcc      120

tgtgtagtct caggattcac tttcagtaat tatggcatgg tctgggtccg ccaggctcca      180

aagaagggtc tggagtgggt cgcatatatt gattctgatg gtgataatac ttactaccga      240

gattccgtga agggccgatt cactatctcc agaaataatg caaaaagcac cctatatttg      300

caaatggaca gtctgaggtc tgaggacacg gccacttatt actgtacaac agggattgtc      360

cggccctttc tctattgggg ccaaggaacc acggtcaccg tctcg                      405


<210>  50
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1519 gL20 V-region

<400>  50

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ser Ser Gln Ser Leu Val Gly Ala 
            20                  25                  30          


Ser Gly Lys Thr Tyr Leu Tyr Trp Leu Phe Gln Lys Pro Gly Lys Ala 
        35                  40                  45              


Pro Lys Arg Leu Ile Tyr Leu Val Ser Thr Leu Asp Ser Gly Ile Pro 
    50                  55                  60                  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile 
65                  70                  75                  80  


Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Leu Gln Gly 
                85                  90                  95      


Thr His Phe Pro His Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


<210>  51
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  1519 gL20 V-region

<400>  51
gatatccaga tgacccagag tccaagcagt ctctccgcca gcgtaggcga tcgtgtgact       60

attacctgta aaagctccca gtccctggtg ggtgcaagcg gcaaaaccta cctgtactgg      120

ctcttccaga aaccgggcaa agctccgaaa cgcctgatct atctggtgtc taccctggat      180

agcggtattc cgtctcgttt ctccggtagc ggtagcggta ccgaattcac gctgaccatt      240

agctccctcc agccggagga ctttgctacc tattactgcc tccagggcac tcattttccg      300

cacactttcg gccagggtac caaactggaa atcaaa                                336


<210>  52
<211>  133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1519 gL20 V-region

<400>  52

Met Lys Lys Thr Ala Ile Ala Ile Ala Val Ala Leu Ala Gly Phe Ala 
1               5                   10                  15      


Thr Val Ala Gln Ala Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Lys Ser Ser Gln 
        35                  40                  45              


Ser Leu Val Gly Ala Ser Gly Lys Thr Tyr Leu Tyr Trp Leu Phe Gln 
    50                  55                  60                  


Lys Pro Gly Lys Ala Pro Lys Arg Leu Ile Tyr Leu Val Ser Thr Leu 
65                  70                  75                  80  


Asp Ser Gly Ile Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Glu 
                85                  90                  95      


Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr 
            100                 105                 110         


Tyr Cys Leu Gln Gly Thr His Phe Pro His Thr Phe Gly Gln Gly Thr 
        115                 120                 125             


Lys Leu Glu Ile Lys 
    130             


<210>  53
<211>  399
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  1519 gL20 V-region

<400>  53
atgaaaaaga cagctatcgc aattgcagtg gccttggctg gtttcgctac cgtagcgcaa       60

gctgatatcc agatgaccca gagtccaagc agtctctccg ccagcgtagg cgatcgtgtg      120

actattacct gtaaaagctc ccagtccctg gtgggtgcaa gcggcaaaac ctacctgtac      180

tggctcttcc agaaaccggg caaagctccg aaacgcctga tctatctggt gtctaccctg      240

gatagcggta ttccgtctcg tttctccggt agcggtagcg gtaccgaatt cacgctgacc      300

attagctccc tccagccgga ggactttgct acctattact gcctccaggg cactcatttt      360

ccgcacactt tcggccaggg taccaaactg gaaatcaaa                             399


<210>  54
<211>  219
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1519 gL20 light chain (V + constant)

<400>  54

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ser Ser Gln Ser Leu Val Gly Ala 
            20                  25                  30          


Ser Gly Lys Thr Tyr Leu Tyr Trp Leu Phe Gln Lys Pro Gly Lys Ala 
        35                  40                  45              


Pro Lys Arg Leu Ile Tyr Leu Val Ser Thr Leu Asp Ser Gly Ile Pro 
    50                  55                  60                  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile 
65                  70                  75                  80  


Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Leu Gln Gly 
                85                  90                  95      


Thr His Phe Pro His Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
        115                 120                 125             


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
    130                 135                 140                 


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
145                 150                 155                 160 


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
                165                 170                 175     


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
            180                 185                 190         


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
        195                 200                 205             


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
    210                 215                 


<210>  55
<211>  657
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  1519 gL20 light chain (V + constant)

<400>  55
gatatccaga tgacccagag tccaagcagt ctctccgcca gcgtaggcga tcgtgtgact       60

attacctgta aaagctccca gtccctggtg ggtgcaagcg gcaaaaccta cctgtactgg      120

ctcttccaga aaccgggcaa agctccgaaa cgcctgatct atctggtgtc taccctggat      180

agcggtattc cgtctcgttt ctccggtagc ggtagcggta ccgaattcac gctgaccatt      240

agctccctcc agccggagga ctttgctacc tattactgcc tccagggcac tcattttccg      300

cacactttcg gccagggtac caaactggaa atcaaacgta cggtagcggc cccatctgtc      360

ttcatcttcc cgccatctga tgagcagttg aaatctggaa ctgcctctgt tgtgtgcctg      420

ctgaataact tctatcccag agaggccaaa gtacagtgga aggtggataa cgccctccaa      480

tcgggtaact cccaggagag tgtcacagag caggacagca aggacagcac ctacagcctc      540

agcagcaccc tgacgctgag caaagcagac tacgagaaac acaaagtcta cgcctgcgaa      600

gtcacccatc agggcctgag ctcaccagta acaaaaagtt ttaatagagg ggagtgt         657


<210>  56
<211>  240
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1519 gL20 light chain

<400>  56

Met Lys Lys Thr Ala Ile Ala Ile Ala Val Ala Leu Ala Gly Phe Ala 
1               5                   10                  15      


Thr Val Ala Gln Ala Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Lys Ser Ser Gln 
        35                  40                  45              


Ser Leu Val Gly Ala Ser Gly Lys Thr Tyr Leu Tyr Trp Leu Phe Gln 
    50                  55                  60                  


Lys Pro Gly Lys Ala Pro Lys Arg Leu Ile Tyr Leu Val Ser Thr Leu 
65                  70                  75                  80  


Asp Ser Gly Ile Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Glu 
                85                  90                  95      


Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr 
            100                 105                 110         


Tyr Cys Leu Gln Gly Thr His Phe Pro His Thr Phe Gly Gln Gly Thr 
        115                 120                 125             


Lys Leu Glu Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe 
    130                 135                 140                 


Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys 
145                 150                 155                 160 


Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val 
                165                 170                 175     


Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln 
            180                 185                 190         


Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser 
        195                 200                 205             


Lys Ala Asp Tyr Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His 
    210                 215                 220                 


Gln Gly Leu Ser Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
225                 230                 235                 240 


<210>  57
<211>  720
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  1519 gL20 light chain

<400>  57
atgaaaaaga cagctatcgc aattgcagtg gccttggctg gtttcgctac cgtagcgcaa       60

gctgatatcc agatgaccca gagtccaagc agtctctccg ccagcgtagg cgatcgtgtg      120

actattacct gtaaaagctc ccagtccctg gtgggtgcaa gcggcaaaac ctacctgtac      180

tggctcttcc agaaaccggg caaagctccg aaacgcctga tctatctggt gtctaccctg      240

gatagcggta ttccgtctcg tttctccggt agcggtagcg gtaccgaatt cacgctgacc      300

attagctccc tccagccgga ggactttgct acctattact gcctccaggg cactcatttt      360

ccgcacactt tcggccaggg taccaaactg gaaatcaaac gtacggtagc ggccccatct      420

gtcttcatct tcccgccatc tgatgagcag ttgaaatctg gaactgcctc tgttgtgtgc      480

ctgctgaata acttctatcc cagagaggcc aaagtacagt ggaaggtgga taacgccctc      540

caatcgggta actcccagga gagtgtcaca gagcaggaca gcaaggacag cacctacagc      600

ctcagcagca ccctgacgct gagcaaagca gactacgaga aacacaaagt ctacgcctgc      660

gaagtcaccc atcagggcct gagctcacca gtaacaaaaa gttttaatag aggggagtgt      720


<210>  58
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1519 gH20 V-region

<400>  58

Glu Val Pro Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Val Ser Gly Phe Thr Phe Ser Asn Tyr 
            20                  25                  30          


Gly Met Val Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Tyr Ile Asp Ser Asp Gly Asp Asn Thr Tyr Tyr Arg Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Ser Ser Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Thr Gly Ile Val Arg Pro Phe Leu Tyr Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser 
        115     


<210>  59
<211>  348
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  1519 gH20 V-region

<400>  59
gaggttccgc tggtcgagtc tggaggcggg cttgtccagc ctggagggag cctgcgtctc       60

tcttgtgcag tatctggctt cacgttctcc aactacggta tggtgtgggt tcgtcaggct      120

ccaggtaaag gtctggaatg ggtggcgtat attgactccg acggcgacaa cacctactat      180

cgcgactctg tgaaaggtcg cttcaccatt tcccgcgata acgccaaatc cagcctgtac      240

ctgcagatga acagcctgcg tgctgaagat actgcggtgt actattgcac cactggcatc      300

gtgcgtccgt ttctgtattg gggtcagggt accctcgtta ctgtctcg                   348


<210>  60
<211>  137
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1519 gH20 V-region

<400>  60

Met Lys Lys Thr Ala Ile Ala Ile Ala Val Ala Leu Ala Gly Phe Ala 
1               5                   10                  15      


Thr Val Ala Gln Ala Glu Val Pro Leu Val Glu Ser Gly Gly Gly Leu 
            20                  25                  30          


Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Val Ser Gly Phe 
        35                  40                  45              


Thr Phe Ser Asn Tyr Gly Met Val Trp Val Arg Gln Ala Pro Gly Lys 
    50                  55                  60                  


Gly Leu Glu Trp Val Ala Tyr Ile Asp Ser Asp Gly Asp Asn Thr Tyr 
65                  70                  75                  80  


Tyr Arg Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala 
                85                  90                  95      


Lys Ser Ser Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr 
            100                 105                 110         


Ala Val Tyr Tyr Cys Thr Thr Gly Ile Val Arg Pro Phe Leu Tyr Trp 
        115                 120                 125             


Gly Gln Gly Thr Leu Val Thr Val Ser 
    130                 135         


<210>  61
<211>  411
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  1519 gH20 V-region

<400>  61
atgaagaaga ctgctatagc aattgcagtg gcgctagctg gtttcgccac cgtggcgcaa       60

gctgaggttc cgctggtcga gtctggaggc gggcttgtcc agcctggagg gagcctgcgt      120

ctctcttgtg cagtatctgg cttcacgttc tccaactacg gtatggtgtg ggttcgtcag      180

gctccaggta aaggtctgga atgggtggcg tatattgact ccgacggcga caacacctac      240

tatcgcgact ctgtgaaagg tcgcttcacc atttcccgcg ataacgccaa atccagcctg      300

tacctgcaga tgaacagcct gcgtgctgaa gatactgcgg tgtactattg caccactggc      360

atcgtgcgtc cgtttctgta ttggggtcag ggtaccctcg ttactgtctc g               411


<210>  62
<211>  228
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1519gH20 Fab' heavy chain (V + human gamma-1 CH1 + hinge)

<400>  62

Glu Val Pro Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Val Ser Gly Phe Thr Phe Ser Asn Tyr 
            20                  25                  30          


Gly Met Val Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Tyr Ile Asp Ser Asp Gly Asp Asn Thr Tyr Tyr Arg Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Ser Ser Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Thr Gly Ile Val Arg Pro Phe Leu Tyr Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu 
        115                 120                 125             


Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys 
    130                 135                 140                 


Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser 
145                 150                 155                 160 


Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser 
                165                 170                 175     


Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser 
            180                 185                 190         


Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn 
        195                 200                 205             


Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His 
    210                 215                 220                 


Thr Cys Ala Ala 
225             


<210>  63
<211>  684
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  1519gH20 Fab' heavy chain (V + human gamma-1 CH1 + hinge)

<400>  63
gaggttccgc tggtcgagtc tggaggcggg cttgtccagc ctggagggag cctgcgtctc       60

tcttgtgcag tatctggctt cacgttctcc aactacggta tggtgtgggt tcgtcaggct      120

ccaggtaaag gtctggaatg ggtggcgtat attgactccg acggcgacaa cacctactat      180

cgcgactctg tgaaaggtcg cttcaccatt tcccgcgata acgccaaatc cagcctgtac      240

ctgcagatga acagcctgcg tgctgaagat actgcggtgt actattgcac cactggcatc      300

gtgcgtccgt ttctgtattg gggtcagggt accctcgtta ctgtctcgag cgcttctaca      360

aagggcccat cggtcttccc cctggcaccc tcctccaaga gcacctctgg gggcacagcg      420

gccctgggct gcctggtcaa ggactacttc cccgaaccgg tgacggtgtc gtggaactca      480

ggcgccctga ccagcggcgt gcacaccttc ccggctgtcc tacagtcctc aggactctac      540

tccctcagca gcgtggtgac cgtgccctcc agcagcttgg gcacccagac ctacatctgc      600

aacgtgaatc acaagcccag caacaccaag gtcgacaaga aagttgagcc caaatcttgt      660

gacaaaactc acacatgcgc cgcg                                             684


<210>  64
<211>  249
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1519 gH20 Fab' heavy chain

<400>  64

Met Lys Lys Thr Ala Ile Ala Ile Ala Val Ala Leu Ala Gly Phe Ala 
1               5                   10                  15      


Thr Val Ala Gln Ala Glu Val Pro Leu Val Glu Ser Gly Gly Gly Leu 
            20                  25                  30          


Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Val Ser Gly Phe 
        35                  40                  45              


Thr Phe Ser Asn Tyr Gly Met Val Trp Val Arg Gln Ala Pro Gly Lys 
    50                  55                  60                  


Gly Leu Glu Trp Val Ala Tyr Ile Asp Ser Asp Gly Asp Asn Thr Tyr 
65                  70                  75                  80  


Tyr Arg Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala 
                85                  90                  95      


Lys Ser Ser Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr 
            100                 105                 110         


Ala Val Tyr Tyr Cys Thr Thr Gly Ile Val Arg Pro Phe Leu Tyr Trp 
        115                 120                 125             


Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro 
    130                 135                 140                 


Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr 
145                 150                 155                 160 


Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr 
                165                 170                 175     


Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro 
            180                 185                 190         


Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr 
        195                 200                 205             


Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn 
    210                 215                 220                 


His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser 
225                 230                 235                 240 


Cys Asp Lys Thr His Thr Cys Ala Ala 
                245                 


<210>  65
<211>  747
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  1519 gH20 Fab' heavy chain

<400>  65
atgaagaaga ctgctatagc aattgcagtg gcgctagctg gtttcgccac cgtggcgcaa       60

gctgaggttc cgctggtcga gtctggaggc gggcttgtcc agcctggagg gagcctgcgt      120

ctctcttgtg cagtatctgg cttcacgttc tccaactacg gtatggtgtg ggttcgtcag      180

gctccaggta aaggtctgga atgggtggcg tatattgact ccgacggcga caacacctac      240

tatcgcgact ctgtgaaagg tcgcttcacc atttcccgcg ataacgccaa atccagcctg      300

tacctgcaga tgaacagcct gcgtgctgaa gatactgcgg tgtactattg caccactggc      360

atcgtgcgtc cgtttctgta ttggggtcag ggtaccctcg ttactgtctc gagcgcttct      420

acaaagggcc catcggtctt ccccctggca ccctcctcca agagcacctc tgggggcaca      480

gcggccctgg gctgcctggt caaggactac ttccccgaac cggtgacggt gtcgtggaac      540

tcaggcgccc tgaccagcgg cgtgcacacc ttcccggctg tcctacagtc ctcaggactc      600

tactccctca gcagcgtggt gaccgtgccc tccagcagct tgggcaccca gacctacatc      660

tgcaacgtga atcacaagcc cagcaacacc aaggtcgaca agaaagttga gcccaaatct      720

tgtgacaaaa ctcacacatg cgccgcg                                          747


<210>  66
<211>  444
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1519gH20 IgG4 heavy chain (V + human gamma-4P constant)

<400>  66

Glu Val Pro Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Val Ser Gly Phe Thr Phe Ser Asn Tyr 
            20                  25                  30          


Gly Met Val Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Tyr Ile Asp Ser Asp Gly Asp Asn Thr Tyr Tyr Arg Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Ser Ser Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Thr Gly Ile Val Arg Pro Phe Leu Tyr Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu 
        115                 120                 125             


Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys 
    130                 135                 140                 


Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser 
145                 150                 155                 160 


Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser 
                165                 170                 175     


Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser 
            180                 185                 190         


Leu Gly Thr Lys Thr Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn 
        195                 200                 205             


Thr Lys Val Asp Lys Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro 
    210                 215                 220                 


Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe 
225                 230                 235                 240 


Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val 
                245                 250                 255     


Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe 
            260                 265                 270         


Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro 
        275                 280                 285             


Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr 
    290                 295                 300                 


Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val 
305                 310                 315                 320 


Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala 
                325                 330                 335     


Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln 
            340                 345                 350         


Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly 
        355                 360                 365             


Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro 
    370                 375                 380                 


Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser 
385                 390                 395                 400 


Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu 
                405                 410                 415     


Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His 
            420                 425                 430         


Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys 
        435                 440                 


<210>  67
<211>  1939
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  1519gH20 IgG4 heavy chain (V + human gamma-4P constant, exons)

<400>  67
gaggtaccac ttgtggaaag cggaggaggt cttgtgcagc ctggaggaag tttacgtctc       60

tcttgtgctg tgtctggctt caccttctcc aattacggaa tggtctgggt cagacaagca      120

cctggaaagg gtcttgaatg ggtggcctat attgactctg acggggacaa cacctactat      180

cgggattccg tgaaaggacg cttcacaatc tcccgagata acgccaagag ctcactgtac      240

ctgcagatga atagcctgag agccgaggat actgccgtgt actattgcac aacgggaatc      300

gttaggcctt ttctgtactg gggacagggc accttggtta ctgtctcgag cgcttctaca      360

aagggcccat ccgtcttccc cctggcgccc tgctccagga gcacctccga gagcacagcc      420

gccctgggct gcctggtcaa ggactacttc cccgaaccgg tgacggtgtc gtggaactca      480

ggcgccctga ccagcggcgt gcacaccttc ccggctgtcc tacagtcctc aggactctac      540

tccctcagca gcgtggtgac cgtgccctcc agcagcttgg gcacgaagac ctacacctgc      600

aacgtagatc acaagcccag caacaccaag gtggacaaga gagttggtga gaggccagca      660

cagggaggga gggtgtctgc tggaagccag gctcagccct cctgcctgga cgcaccccgg      720

ctgtgcagcc ccagcccagg gcagcaaggc atgccccatc tgtctcctca cccggaggcc      780

tctgaccacc ccactcatgc ccagggagag ggtcttctgg atttttccac caggctccgg      840

gcagccacag gctggatgcc cctaccccag gccctgcgca tacaggggca ggtgctgcgc      900

tcagacctgc caagagccat atccgggagg accctgcccc tgacctaagc ccaccccaaa      960

ggccaaactc tccactccct cagctcagac accttctctc ctcccagatc tgagtaactc     1020

ccaatcttct ctctgcagag tccaaatatg gtcccccatg cccaccatgc ccaggtaagc     1080

caacccaggc ctcgccctcc agctcaaggc gggacaggtg ccctagagta gcctgcatcc     1140

agggacaggc cccagccggg tgctgacgca tccacctcca tctcttcctc agcacctgag     1200

ttcctggggg gaccatcagt cttcctgttc cccccaaaac ccaaggacac tctcatgatc     1260

tcccggaccc ctgaggtcac gtgcgtggtg gtggacgtga gccaggaaga ccccgaggtc     1320

cagttcaact ggtacgtgga tggcgtggag gtgcataatg ccaagacaaa gccgcgggag     1380

gagcagttca acagcacgta ccgtgtggtc agcgtcctca ccgtcctgca ccaggactgg     1440

ctgaacggca aggagtacaa gtgcaaggtc tccaacaaag gcctcccgtc ctccatcgag     1500

aaaaccatct ccaaagccaa aggtgggacc cacggggtgc gagggccaca tggacagagg     1560

tcagctcggc ccaccctctg ccctgggagt gaccgctgtg ccaacctctg tccctacagg     1620

gcagccccga gagccacagg tgtacaccct gcccccatcc caggaggaga tgaccaagaa     1680

ccaggtcagc ctgacctgcc tggtcaaagg cttctacccc agcgacatcg ccgtggagtg     1740

ggagagcaat gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga     1800

cggctccttc ttcctctaca gcaggctaac cgtggacaag agcaggtggc aggaggggaa     1860

tgtcttctca tgctccgtga tgcatgaggc tctgcacaac cactacacac agaagagcct     1920

ctccctgtct ctgggtaaa                                                  1939


<210>  68
<211>  347
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1519gL20 FabFv light chain

<400>  68

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ser Ser Gln Ser Leu Val Gly Ala 
            20                  25                  30          


Ser Gly Lys Thr Tyr Leu Tyr Trp Leu Phe Gln Lys Pro Gly Lys Ala 
        35                  40                  45              


Pro Lys Arg Leu Ile Tyr Leu Val Ser Thr Leu Asp Ser Gly Ile Pro 
    50                  55                  60                  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile 
65                  70                  75                  80  


Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Leu Gln Gly 
                85                  90                  95      


Thr His Phe Pro His Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
        115                 120                 125             


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
    130                 135                 140                 


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
145                 150                 155                 160 


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
                165                 170                 175     


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
            180                 185                 190         


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
        195                 200                 205             


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys Ser Gly Gly Gly Gly 
    210                 215                 220                 


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr 
225                 230                 235                 240 


Gln Ser Pro Ser Ser Val Ser Ala Ser Val Gly Asp Arg Val Thr Ile 
                245                 250                 255     


Thr Cys Gln Ser Ser Pro Ser Val Trp Ser Asn Phe Leu Ser Trp Tyr 
            260                 265                 270         


Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Glu Ala Ser 
        275                 280                 285             


Lys Leu Thr Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly 
    290                 295                 300                 


Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala 
305                 310                 315                 320 


Thr Tyr Tyr Cys Gly Gly Gly Tyr Ser Ser Ile Ser Asp Thr Thr Phe 
                325                 330                 335     


Gly Cys Gly Thr Lys Val Glu Ile Lys Arg Thr 
            340                 345         


<210>  69
<211>  1041
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  1519gL20 FabFv light chain

<400>  69
gatatccaga tgacccagag cccatctagc ttatccgctt ccgttggtga tcgcgtgaca       60

attacgtgta agagctccca atctctcgtg ggtgcaagtg gcaagaccta tctgtactgg      120

ctctttcaga agcctggcaa ggcaccaaaa cggctgatct atctggtgtc tacccttgac      180

tctgggatac cgtcacgatt ttccggatct gggagcggaa ctgagttcac actcacgatt      240

tcatcgctgc aacccgagga ctttgctacc tactactgcc tgcaaggcac tcatttccct      300

cacactttcg gccaggggac aaaactcgaa atcaaacgta cggtagcggc cccatctgtc      360

ttcatcttcc cgccatctga tgagcagttg aaatctggaa ctgcctctgt tgtgtgcctg      420

ctgaataact tctatcccag agaggccaaa gtacagtgga aggtggataa cgccctccaa      480

tcgggtaact cccaggagag tgtcacagag caggacagca aggacagcac ctacagcctg      540

agcagcaccc tgacgctgtc taaagcagac tacgagaaac acaaagtgta cgcctgcgaa      600

gtcacccatc agggcctgag ctcaccagta acaaaaagtt ttaatagagg ggagtgtagc      660

ggtggcggtg gcagtggtgg gggaggctcc ggaggtggcg gttcagacat acaaatgacc      720

cagagtcctt catcggtatc cgcgtccgtt ggcgataggg tgactattac atgtcaaagc      780

tctcctagcg tctggagcaa ttttctatcc tggtatcaac agaaaccggg gaaggctcca      840

aaacttctga tttatgaagc ctcgaaactc accagtggag ttccgtcaag attcagtggc      900

tctggatcag ggacagactt cacgttgaca atcagttcgc tgcaaccaga ggactttgcg      960

acctactatt gtggtggagg ttacagtagc ataagtgata cgacatttgg gtgcggtact     1020

aaggtggaaa tcaaacgtac c                                               1041


<210>  70
<211>  367
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1519gL20 FabFv light chain

<400>  70

Met Ser Val Pro Thr Gln Val Leu Gly Leu Leu Leu Leu Trp Leu Thr 
1               5                   10                  15      


Asp Ala Arg Cys Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser 
            20                  25                  30          


Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Lys Ser Ser Gln Ser 
        35                  40                  45              


Leu Val Gly Ala Ser Gly Lys Thr Tyr Leu Tyr Trp Leu Phe Gln Lys 
    50                  55                  60                  


Pro Gly Lys Ala Pro Lys Arg Leu Ile Tyr Leu Val Ser Thr Leu Asp 
65                  70                  75                  80  


Ser Gly Ile Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Glu Phe 
                85                  90                  95      


Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr 
            100                 105                 110         


Cys Leu Gln Gly Thr His Phe Pro His Thr Phe Gly Gln Gly Thr Lys 
        115                 120                 125             


Leu Glu Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro 
    130                 135                 140                 


Pro Ser Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu 
145                 150                 155                 160 


Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp 
                165                 170                 175     


Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp 
            180                 185                 190         


Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys 
        195                 200                 205             


Ala Asp Tyr Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln 
    210                 215                 220                 


Gly Leu Ser Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys Ser 
225                 230                 235                 240 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp 
                245                 250                 255     


Ile Gln Met Thr Gln Ser Pro Ser Ser Val Ser Ala Ser Val Gly Asp 
            260                 265                 270         


Arg Val Thr Ile Thr Cys Gln Ser Ser Pro Ser Val Trp Ser Asn Phe 
        275                 280                 285             


Leu Ser Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
    290                 295                 300                 


Tyr Glu Ala Ser Lys Leu Thr Ser Gly Val Pro Ser Arg Phe Ser Gly 
305                 310                 315                 320 


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
                325                 330                 335     


Glu Asp Phe Ala Thr Tyr Tyr Cys Gly Gly Gly Tyr Ser Ser Ile Ser 
            340                 345                 350         


Asp Thr Thr Phe Gly Cys Gly Thr Lys Val Glu Ile Lys Arg Thr 
        355                 360                 365         


<210>  71
<211>  1101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  1519gL20 FabFv light chain

<400>  71
atgtctgtcc ccacccaagt cctcggactc ctgctactct ggcttacaga tgccagatgc       60

gatatccaga tgacccagag cccatctagc ttatccgctt ccgttggtga tcgcgtgaca      120

attacgtgta agagctccca atctctcgtg ggtgcaagtg gcaagaccta tctgtactgg      180

ctctttcaga agcctggcaa ggcaccaaaa cggctgatct atctggtgtc tacccttgac      240

tctgggatac cgtcacgatt ttccggatct gggagcggaa ctgagttcac actcacgatt      300

tcatcgctgc aacccgagga ctttgctacc tactactgcc tgcaaggcac tcatttccct      360

cacactttcg gccaggggac aaaactcgaa atcaaacgta cggtagcggc cccatctgtc      420

ttcatcttcc cgccatctga tgagcagttg aaatctggaa ctgcctctgt tgtgtgcctg      480

ctgaataact tctatcccag agaggccaaa gtacagtgga aggtggataa cgccctccaa      540

tcgggtaact cccaggagag tgtcacagag caggacagca aggacagcac ctacagcctg      600

agcagcaccc tgacgctgtc taaagcagac tacgagaaac acaaagtgta cgcctgcgaa      660

gtcacccatc agggcctgag ctcaccagta acaaaaagtt ttaatagagg ggagtgtagc      720

ggtggcggtg gcagtggtgg gggaggctcc ggaggtggcg gttcagacat acaaatgacc      780

cagagtcctt catcggtatc cgcgtccgtt ggcgataggg tgactattac atgtcaaagc      840

tctcctagcg tctggagcaa ttttctatcc tggtatcaac agaaaccggg gaaggctcca      900

aaacttctga tttatgaagc ctcgaaactc accagtggag ttccgtcaag attcagtggc      960

tctggatcag ggacagactt cacgttgaca atcagttcgc tgcaaccaga ggactttgcg     1020

acctactatt gtggtggagg ttacagtagc ataagtgata cgacatttgg gtgcggtact     1080

aaggtggaaa tcaaacgtac c                                               1101


<210>  72
<211>  357
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1519gH20 FabFv heavy chain

<400>  72

Glu Val Pro Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Val Ser Gly Phe Thr Phe Ser Asn Tyr 
            20                  25                  30          


Gly Met Val Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Tyr Ile Asp Ser Asp Gly Asp Asn Thr Tyr Tyr Arg Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Ser Ser Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Thr Gly Ile Val Arg Pro Phe Leu Tyr Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu 
        115                 120                 125             


Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys 
    130                 135                 140                 


Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser 
145                 150                 155                 160 


Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser 
                165                 170                 175     


Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser 
            180                 185                 190         


Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn 
        195                 200                 205             


Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Ser Gly Gly Gly 
    210                 215                 220                 


Gly Ser Gly Gly Gly Gly Thr Gly Gly Gly Gly Ser Glu Val Gln Leu 
225                 230                 235                 240 


Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu 
                245                 250                 255     


Ser Cys Ala Val Ser Gly Ile Asp Leu Ser Asn Tyr Ala Ile Asn Trp 
            260                 265                 270         


Val Arg Gln Ala Pro Gly Lys Cys Leu Glu Trp Ile Gly Ile Ile Trp 
        275                 280                 285             


Ala Ser Gly Thr Thr Phe Tyr Ala Thr Trp Ala Lys Gly Arg Phe Thr 
    290                 295                 300                 


Ile Ser Arg Asp Asn Ser Lys Asn Thr Val Tyr Leu Gln Met Asn Ser 
305                 310                 315                 320 


Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Thr Val Pro 
                325                 330                 335     


Gly Tyr Ser Thr Ala Pro Tyr Phe Asp Leu Trp Gly Gln Gly Thr Leu 
            340                 345                 350         


Val Thr Val Ser Ser 
        355         


<210>  73
<211>  1071
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  1519gH20 FabFv heavy chain

<400>  73
gaggtaccac ttgtggaaag cggaggaggt cttgtgcagc ctggaggaag tttacgtctc       60

tcttgtgctg tgtctggctt caccttctcc aattacggaa tggtctgggt cagacaagca      120

cctggaaagg gtcttgaatg ggtggcctat attgactctg acggggacaa cacctactat      180

cgggattccg tgaaaggacg cttcacaatc tcccgagata acgccaagag ctcactgtac      240

ctgcagatga atagcctgag agccgaggat actgccgtgt actattgcac aacgggaatc      300

gttaggcctt ttctgtactg gggacagggc accttggtta ctgtctcgag cgcgtccaca      360

aagggcccat cggtcttccc cctggcaccc tcctccaaga gcacctctgg gggcacagcg      420

gccctgggct gcctggtcaa ggactacttc cccgaaccag tgacggtgtc gtggaactca      480

ggtgccctga ccagcggcgt tcacaccttc ccggctgtcc tacagtcttc aggactctac      540

tccctgagca gcgtggtgac cgtgccctcc agcagcttgg gcacccagac ctacatctgc      600

aacgtgaatc acaagcccag caacaccaag gtcgataaga aagttgagcc caaatcttgt      660

agtggaggtg ggggctcagg tggaggcggg accggtggag gtggcagcga ggttcaactg      720

cttgagtctg gaggaggcct agtccagcct ggagggagcc tgcgtctctc ttgtgcagta      780

agcggcatcg acctgagcaa ttacgccatc aactgggtga gacaagctcc ggggaagtgt      840

ttagaatgga tcggtataat atgggccagt gggacgacct tttatgctac atgggcgaaa      900

ggaaggttta caattagccg ggacaatagc aaaaacaccg tgtatctcca aatgaactcc      960

ttgcgagcag aggacacggc ggtgtactat tgtgctcgca ctgtcccagg ttatagcact     1020

gcaccctact tcgatctgtg gggacaaggg accctggtga ctgtttcaag t              1071


<210>  74
<211>  376
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1519gH20 FabFv heavy chain

<400>  74

Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      


Val His Ser Glu Val Pro Leu Val Glu Ser Gly Gly Gly Leu Val Gln 
            20                  25                  30          


Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Val Ser Gly Phe Thr Phe 
        35                  40                  45              


Ser Asn Tyr Gly Met Val Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
    50                  55                  60                  


Glu Trp Val Ala Tyr Ile Asp Ser Asp Gly Asp Asn Thr Tyr Tyr Arg 
65                  70                  75                  80  


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Ser 
                85                  90                  95      


Ser Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val 
            100                 105                 110         


Tyr Tyr Cys Thr Thr Gly Ile Val Arg Pro Phe Leu Tyr Trp Gly Gln 
        115                 120                 125             


Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val 
    130                 135                 140                 


Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala 
145                 150                 155                 160 


Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser 
                165                 170                 175     


Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val 
            180                 185                 190         


Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro 
        195                 200                 205             


Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys 
    210                 215                 220                 


Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Ser 
225                 230                 235                 240 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Thr Gly Gly Gly Gly Ser Glu 
                245                 250                 255     


Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser 
            260                 265                 270         


Leu Arg Leu Ser Cys Ala Val Ser Gly Ile Asp Leu Ser Asn Tyr Ala 
        275                 280                 285             


Ile Asn Trp Val Arg Gln Ala Pro Gly Lys Cys Leu Glu Trp Ile Gly 
    290                 295                 300                 


Ile Ile Trp Ala Ser Gly Thr Thr Phe Tyr Ala Thr Trp Ala Lys Gly 
305                 310                 315                 320 


Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Val Tyr Leu Gln 
                325                 330                 335     


Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg 
            340                 345                 350         


Thr Val Pro Gly Tyr Ser Thr Ala Pro Tyr Phe Asp Leu Trp Gly Gln 
        355                 360                 365             


Gly Thr Leu Val Thr Val Ser Ser 
    370                 375     


<210>  75
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Wild type Tsp 5'

<400>  75

Met Asn Met Phe Phe Arg Leu Thr Ala Leu Ala Gly Leu Leu Ala Ile 
1               5                   10                  15      


Ala Gly Gln Thr Phe Ala 
            20          


<210>  76
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Wild type Tsp 5'

<400>  76
atgaacatgt tttttaggct taccgcgtta gctggcctgc ttgcaatagc aggccagacc       60

ttcgct                                                                  66


<210>  77
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated  Tsp 5' fragment

<400>  77

Met Asn Ser Phe Leu Gly Leu Pro Arg Leu Ala Cys Leu Gln Gln Ala 
1               5                   10                  15      


Arg His Leu 
            


<210>  78
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutated Tsp 5' fragment

<400>  78
atgaattcgt ttttaggctt accgcgttag ctggcctgct tgcaatagca ggccagacat       60

taattg                                                                  66


<210>  79
<211>  20
<212>  PRT
<213>  Artificial

<220>
<223>  Mutated delta ptr (protease III) 5'

<400>  79

Ile Pro Arg Ser Thr Trp Phe Lys Ala Leu Leu Leu Leu Val Ala Leu 
1               5                   10                  15      


Trp Ala His Cys 
            20  


<210>  80
<211>  66
<212>  DNA
<213>  Artificial

<220>
<223>  Mutated delta ptr (protease III) 5'

<400>  80
tgaattcccc gcagcacctg gttcaaagca ttattgttgt tagttgccct ttgggcacat       60

taatgt                                                                  66


<210>  81
<211>  21
<212>  PRT
<213>  Artificial

<220>
<223>  Wild-type ptr (protease III) 5'

<400>  81

Met Pro Arg Ser Thr Trp Phe Lys Ala Leu Leu Leu Leu Val Ala Leu 
1               5                   10                  15      


Trp Ala Pro Leu Ser 
            20      


<210>  82
<211>  66
<212>  DNA
<213>  Artificial

<220>
<223>  Wild-type ptr (protease III) 5'

<400>  82
tgaatgcccc gcagcacctg gttcaaagca ttattgttgt tagttgccct ttgggcaccc       60

ttaagt                                                                  66


