                         SEQUENCE LISTING

<110>  THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE 
       SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES
       Kopecko, Dennis J.
       Osorio, Manuel
       Foote, Monica R.
 
<120>  SALMONELLA TYPHI TY21A EXPRESSING YERSINIA PESTIS F1-V FUSION 
       PROTEIN AND USES THEREOF

<130>  4239-88264-02

<150>  US 61/650,676
<151>  2012-05-23

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  1747
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide


<220>
<221>  promoter
<222>  (1)..(112)

<220>
<221>  CDS
<222>  (113)..(562)
<223>  F1 antigen coding sequence

<220>
<221>  CDS
<222>  (563)..(580)
<223>  linker sequence

<220>
<221>  CDS
<222>  (581)..(1558)
<223>  V antigen coding sequence

<220>
<221>  CDS
<222>  (1559)..(1747)
<223>  hlyA secretion signal sequence

<400>  1
tggacttttg taaagatgga caataaattt ttactttttc cagaaacttt attccggaac       60

ttcgcgttat aaaatgaatc tgacgtacac agcaatttta aggagaggac at atg gca      118
                                                          Met Ala         
                                                          1               

gat tta act gca agc acc act gca acg gca act ctt gtt gaa cca gcc        166
Asp Leu Thr Ala Ser Thr Thr Ala Thr Ala Thr Leu Val Glu Pro Ala           
        5                   10                  15                        

cgc atc act ctt aca tat aag gaa ggc gct cca att aca att atg gac        214
Arg Ile Thr Leu Thr Tyr Lys Glu Gly Ala Pro Ile Thr Ile Met Asp           
    20                  25                  30                            

aat gga aac atc gat aca gaa tta ctt gtt ggt acg ctt act ctt ggc        262
Asn Gly Asn Ile Asp Thr Glu Leu Leu Val Gly Thr Leu Thr Leu Gly           
35                  40                  45                  50            

ggc tat aaa aca gga acc act agc aca tct gtt aac ttt aca gat gcc        310
Gly Tyr Lys Thr Gly Thr Thr Ser Thr Ser Val Asn Phe Thr Asp Ala           
                55                  60                  65                

gcg ggt gat ccc atg tac tta aca ttt act tct cag gat gga aat aac        358
Ala Gly Asp Pro Met Tyr Leu Thr Phe Thr Ser Gln Asp Gly Asn Asn           
            70                  75                  80                    

cac caa ttc act aca aaa gtg att ggc aag gat tct aga gat ttt gat        406
His Gln Phe Thr Thr Lys Val Ile Gly Lys Asp Ser Arg Asp Phe Asp           
        85                  90                  95                        

atc tct cct aag gta aac ggt gag aac ctt gtg ggg gat gac gtc gtc        454
Ile Ser Pro Lys Val Asn Gly Glu Asn Leu Val Gly Asp Asp Val Val           
    100                 105                 110                           

ttg gct acg ggc agc cag gat ttc ttt gtt cgc tca att ggt tcc aaa        502
Leu Ala Thr Gly Ser Gln Asp Phe Phe Val Arg Ser Ile Gly Ser Lys           
115                 120                 125                 130           

ggc ggt aaa ctt gca gca ggt aaa tac act gat gct gta acc gta acc        550
Gly Gly Lys Leu Ala Ala Gly Lys Tyr Thr Asp Ala Val Thr Val Thr           
                135                 140                 145               

gta tct aac caa gga tcc atc gaa ggt cgt atg att aga gcc tac gaa        598
Val Ser Asn Gln Gly Ser Ile Glu Gly Arg Met Ile Arg Ala Tyr Glu           
            150                 155                 160                   

caa aac cca caa cat ttt att gag gat cta gaa aaa gtt agg gtg gaa        646
Gln Asn Pro Gln His Phe Ile Glu Asp Leu Glu Lys Val Arg Val Glu           
        165                 170                 175                       

caa ctt act ggt cat ggt tct tca gtt tta gaa gaa ttg gtt cag tta        694
Gln Leu Thr Gly His Gly Ser Ser Val Leu Glu Glu Leu Val Gln Leu           
    180                 185                 190                           

gtc aaa gat aaa aat ata gat att tcc att aaa tat gat ccc aga aaa        742
Val Lys Asp Lys Asn Ile Asp Ile Ser Ile Lys Tyr Asp Pro Arg Lys           
195                 200                 205                 210           

gat tcg gag gtt ttt gcc aat aga gta att act gat gat atc gaa ttg        790
Asp Ser Glu Val Phe Ala Asn Arg Val Ile Thr Asp Asp Ile Glu Leu           
                215                 220                 225               

ctc aag aaa atc cta gct tat ttt cta ccc gag gat gcc att ctt aaa        838
Leu Lys Lys Ile Leu Ala Tyr Phe Leu Pro Glu Asp Ala Ile Leu Lys           
            230                 235                 240                   

ggc ggt cat tat gac aac caa ctg caa aat ggc atc aag cga gta aaa        886
Gly Gly His Tyr Asp Asn Gln Leu Gln Asn Gly Ile Lys Arg Val Lys           
        245                 250                 255                       

gag ttc ctt gaa tca tcg ccg aat aca caa tgg gaa ttg cgg gcg ttc        934
Glu Phe Leu Glu Ser Ser Pro Asn Thr Gln Trp Glu Leu Arg Ala Phe           
    260                 265                 270                           

atg gca gta atg cac ttc tct tta acc gcc gat cgt atc gat gat gat        982
Met Ala Val Met His Phe Ser Leu Thr Ala Asp Arg Ile Asp Asp Asp           
275                 280                 285                 290           

att ttg aaa gtg att gtt gat tca atg aat cat cat ggt gat gcc cgt       1030
Ile Leu Lys Val Ile Val Asp Ser Met Asn His His Gly Asp Ala Arg           
                295                 300                 305               

agc aag ttg cgt gaa gaa tta gct gag ctt acc gcc gaa tta aag att       1078
Ser Lys Leu Arg Glu Glu Leu Ala Glu Leu Thr Ala Glu Leu Lys Ile           
            310                 315                 320                   

tat tca gtt att caa gcc gaa att aat aag cat ctg tct agt agt ggc       1126
Tyr Ser Val Ile Gln Ala Glu Ile Asn Lys His Leu Ser Ser Ser Gly           
        325                 330                 335                       

acc ata aat atc cat gat aaa tcc att aat ctc atg gat aaa aat tta       1174
Thr Ile Asn Ile His Asp Lys Ser Ile Asn Leu Met Asp Lys Asn Leu           
    340                 345                 350                           

tat ggt tat aca gat gaa gag att ttt aaa gcc agc gca gag tac aaa       1222
Tyr Gly Tyr Thr Asp Glu Glu Ile Phe Lys Ala Ser Ala Glu Tyr Lys           
355                 360                 365                 370           

att ctc gag aaa atg cct caa acc acc att cag gtg gat ggg agc gag       1270
Ile Leu Glu Lys Met Pro Gln Thr Thr Ile Gln Val Asp Gly Ser Glu           
                375                 380                 385               

aaa aaa ata gtc tcg ata aag gac ttt ctt gga agt gag aat aaa aga       1318
Lys Lys Ile Val Ser Ile Lys Asp Phe Leu Gly Ser Glu Asn Lys Arg           
            390                 395                 400                   

acc ggg gcg ttg ggt aat ctg aaa aac tca tac tct tat aat aaa gat       1366
Thr Gly Ala Leu Gly Asn Leu Lys Asn Ser Tyr Ser Tyr Asn Lys Asp           
        405                 410                 415                       

aat aat gaa tta tct cac ttt gcc acc acc tgc tcg gat aag tcc agg       1414
Asn Asn Glu Leu Ser His Phe Ala Thr Thr Cys Ser Asp Lys Ser Arg           
    420                 425                 430                           

ccg ctc aac gac ttg gtt agc caa aaa aca act cag ctg tct gat att       1462
Pro Leu Asn Asp Leu Val Ser Gln Lys Thr Thr Gln Leu Ser Asp Ile           
435                 440                 445                 450           

aca tca cgt ttt aat tca gct att gaa gca ctg aac cgt ttc att cag       1510
Thr Ser Arg Phe Asn Ser Ala Ile Glu Ala Leu Asn Arg Phe Ile Gln           
                455                 460                 465               

aaa tat gat tca gtg atg caa cgt ctg cta gat gac acg tct ggt aaa       1558
Lys Tyr Asp Ser Val Met Gln Arg Leu Leu Asp Asp Thr Ser Gly Lys           
            470                 475                 480                   

tat gca tca act tat gca gac ctg gat aat ctg aat cca tta att aat       1606
Tyr Ala Ser Thr Tyr Ala Asp Leu Asp Asn Leu Asn Pro Leu Ile Asn           
        485                 490                 495                       

gaa atc agc aaa att att tca gct gca ggt aac ttc gat gtt aag gag       1654
Glu Ile Ser Lys Ile Ile Ser Ala Ala Gly Asn Phe Asp Val Lys Glu           
    500                 505                 510                           

gaa aga tct gcc gct tct tta ttg cag ttg tcc ggt aat gcc agt gat       1702
Glu Arg Ser Ala Ala Ser Leu Leu Gln Leu Ser Gly Asn Ala Ser Asp           
515                 520                 525                 530           

ttt tca tat gga cgg aac tca ata act ttg acc gca tca gca taa           1747
Phe Ser Tyr Gly Arg Asn Ser Ile Thr Leu Thr Ala Ser Ala                   
                535                 540                                   


<210>  2
<211>  150
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  2

Met Ala Asp Leu Thr Ala Ser Thr Thr Ala Thr Ala Thr Leu Val Glu 
1               5                   10                  15      


Pro Ala Arg Ile Thr Leu Thr Tyr Lys Glu Gly Ala Pro Ile Thr Ile 
            20                  25                  30          


Met Asp Asn Gly Asn Ile Asp Thr Glu Leu Leu Val Gly Thr Leu Thr 
        35                  40                  45              


Leu Gly Gly Tyr Lys Thr Gly Thr Thr Ser Thr Ser Val Asn Phe Thr 
    50                  55                  60                  


Asp Ala Ala Gly Asp Pro Met Tyr Leu Thr Phe Thr Ser Gln Asp Gly 
65                  70                  75                  80  


Asn Asn His Gln Phe Thr Thr Lys Val Ile Gly Lys Asp Ser Arg Asp 
                85                  90                  95      


Phe Asp Ile Ser Pro Lys Val Asn Gly Glu Asn Leu Val Gly Asp Asp 
            100                 105                 110         


Val Val Leu Ala Thr Gly Ser Gln Asp Phe Phe Val Arg Ser Ile Gly 
        115                 120                 125             


Ser Lys Gly Gly Lys Leu Ala Ala Gly Lys Tyr Thr Asp Ala Val Thr 
    130                 135                 140                 


Val Thr Val Ser Asn Gln 
145                 150 


<210>  3
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  3

Gly Ser Ile Glu Gly Arg 
1               5       


<210>  4
<211>  326
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  4

Met Ile Arg Ala Tyr Glu Gln Asn Pro Gln His Phe Ile Glu Asp Leu 
1               5                   10                  15      


Glu Lys Val Arg Val Glu Gln Leu Thr Gly His Gly Ser Ser Val Leu 
            20                  25                  30          


Glu Glu Leu Val Gln Leu Val Lys Asp Lys Asn Ile Asp Ile Ser Ile 
        35                  40                  45              


Lys Tyr Asp Pro Arg Lys Asp Ser Glu Val Phe Ala Asn Arg Val Ile 
    50                  55                  60                  


Thr Asp Asp Ile Glu Leu Leu Lys Lys Ile Leu Ala Tyr Phe Leu Pro 
65                  70                  75                  80  


Glu Asp Ala Ile Leu Lys Gly Gly His Tyr Asp Asn Gln Leu Gln Asn 
                85                  90                  95      


Gly Ile Lys Arg Val Lys Glu Phe Leu Glu Ser Ser Pro Asn Thr Gln 
            100                 105                 110         


Trp Glu Leu Arg Ala Phe Met Ala Val Met His Phe Ser Leu Thr Ala 
        115                 120                 125             


Asp Arg Ile Asp Asp Asp Ile Leu Lys Val Ile Val Asp Ser Met Asn 
    130                 135                 140                 


His His Gly Asp Ala Arg Ser Lys Leu Arg Glu Glu Leu Ala Glu Leu 
145                 150                 155                 160 


Thr Ala Glu Leu Lys Ile Tyr Ser Val Ile Gln Ala Glu Ile Asn Lys 
                165                 170                 175     


His Leu Ser Ser Ser Gly Thr Ile Asn Ile His Asp Lys Ser Ile Asn 
            180                 185                 190         


Leu Met Asp Lys Asn Leu Tyr Gly Tyr Thr Asp Glu Glu Ile Phe Lys 
        195                 200                 205             


Ala Ser Ala Glu Tyr Lys Ile Leu Glu Lys Met Pro Gln Thr Thr Ile 
    210                 215                 220                 


Gln Val Asp Gly Ser Glu Lys Lys Ile Val Ser Ile Lys Asp Phe Leu 
225                 230                 235                 240 


Gly Ser Glu Asn Lys Arg Thr Gly Ala Leu Gly Asn Leu Lys Asn Ser 
                245                 250                 255     


Tyr Ser Tyr Asn Lys Asp Asn Asn Glu Leu Ser His Phe Ala Thr Thr 
            260                 265                 270         


Cys Ser Asp Lys Ser Arg Pro Leu Asn Asp Leu Val Ser Gln Lys Thr 
        275                 280                 285             


Thr Gln Leu Ser Asp Ile Thr Ser Arg Phe Asn Ser Ala Ile Glu Ala 
    290                 295                 300                 


Leu Asn Arg Phe Ile Gln Lys Tyr Asp Ser Val Met Gln Arg Leu Leu 
305                 310                 315                 320 


Asp Asp Thr Ser Gly Lys 
                325     


<210>  5
<211>  62
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  5

Tyr Ala Ser Thr Tyr Ala Asp Leu Asp Asn Leu Asn Pro Leu Ile Asn 
1               5                   10                  15      


Glu Ile Ser Lys Ile Ile Ser Ala Ala Gly Asn Phe Asp Val Lys Glu 
            20                  25                  30          


Glu Arg Ser Ala Ala Ser Leu Leu Gln Leu Ser Gly Asn Ala Ser Asp 
        35                  40                  45              


Phe Ser Tyr Gly Arg Asn Ser Ile Thr Leu Thr Ala Ser Ala 
    50                  55                  60          


