                         SEQUENCE LISTING

<110>  Ophthotech Corp.
       Everett, Richard
       Chang, Byeong Seon
 
<120>  COMPOSITIONS COMPRISING AN ANTI-PDGF APTAMER AND A VEGF 
       ANTAGONIST

<130>  OPHT-010/02WO 315137-2198

<150>  US 61/778,208
<151>  2013-03-12

<150>  US 61/654,672
<151>  2012-06-01

<160>  85    

<170>  PatentIn version 3.5

<210>  1
<211>  9
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  modified_base
<222>  (6)..(6)
<223>  uridine is 2'-fluoro

<220>
<221>  modified_base
<222>  (8)..(8)
<223>  cytidine is 2'-fluoro

<220>
<221>  modified_base
<222>  (9)..(9)
<223>  gm

<400>  1
caggcuacg                                                                9


<210>  2
<211>  12
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  modified_base
<222>  (5)..(5)
<223>  gm

<220>
<221>  modified_base
<222>  (7)..(7)
<223>  gm

<220>
<221>  modified_base
<222>  (10)..(10)
<223>  uridine is 2'-fluoro

<220>
<221>  modified_base
<222>  (11)..(11)
<223>  cytidine is 2'-fluoro

<220>
<221>  modified_base
<222>  (12)..(12)
<223>  adenosine is 2'-O-Methyl

<400>  2
cgtagagcau ca                                                           12


<210>  3
<211>  9
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  modified_base
<222>  (5)..(6)
<223>  cytidine is 2'-fluoro

<220>
<221>  modified_base
<222>  (7)..(7)
<223>  uridine is 2'-fluoro

<220>
<221>  modified_base
<222>  (8)..(8)
<223>  gm

<220>
<221>  modified_base
<222>  (9)..(9)
<223>  inverted thymidine

<400>  3
tgatccugt                                                                9


<210>  4
<211>  57
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  modified_base
<222>  (10)..(32)
<223>  From nucleotides 10 to 32, all purines (A and G) and all 
       pyrimdines (C and U) are 2'-O-Methyl

<400>  4
caggctacga ugcaguuuga gaagucgcgc aucgtagagc atcagaaatg atcctgt          57


<210>  5
<211>  55
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  modified_base
<222>  (10)..(30)
<223>  From nucleotides 10 to 30, all purines (A and G) and all 
       pyrimdines (C and U) are 2'-O-Methyl

<400>  5
caggctacgu gcaguuugag aagucgcgca cgtagagcat cagaaatgat cctgt            55


<210>  6
<211>  40
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer

<400>  6
cacaggctac ggcacgtaga gcatcaccat gatcctgtgt                             40


<210>  7
<211>  22
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  misc_feature
<222>  (1)..(22)
<223>  phosphorothioate backbone

<400>  7
tgactgtgaa cgttcgagat ga                                                22


<210>  8
<211>  14
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  misc_feature
<222>  (1)..(14)
<223>  phosphorothioate backbone

<400>  8
tgaacgttcg agat                                                         14


<210>  9
<211>  12
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  misc_feature
<222>  (1)..(12)
<223>  phosphorothioate backbone

<400>  9
aacgttcgag at                                                           12


<210>  10
<211>  10
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  misc_feature
<222>  (1)..(10)
<223>  phosphorothioate backbone

<400>  10
aacgttcgag                                                              10


<210>  11
<211>  13
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  misc_feature
<222>  (1)..(13)
<223>  phosphorothioate backbone

<400>  11
gtgaacgttc gag                                                          13


<210>  12
<211>  24
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  misc_feature
<222>  (1)..(24)
<223>  phosphorothioate backbone

<400>  12
tcgtcgtttt gtcgttttgt cgtt                                              24


<210>  13
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  misc_feature
<222>  (1)..(18)
<223>  phosphorothioate backbone

<400>  13
gtcgttttgt cgttttgt                                                     18


<210>  14
<211>  14
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  misc_feature
<222>  (1)..(14)
<223>  phosphorothioate backbone

<400>  14
gtcgttttgt cgtt                                                         14


<210>  15
<211>  46
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  misc_feature
<222>  (1)..(11)
<223>  phosphorothiate backbone between nucleotides 1-11

<220>
<221>  misc_feature
<222>  (44)..(46)
<223>  phosphorothiate backbone between nucleotides 44-46

<400>  15
aacgttcgag caggctacgg cacgtagagc atcaccatga tcctgc                      46


<210>  16
<211>  49
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  misc_feature
<222>  (1)..(14)
<223>  phosphorothioate backbone between nucleotides 1-14

<220>
<221>  misc_feature
<222>  (47)..(49)
<223>  phosphorothioate backbone between nucleotides 47-49

<400>  16
gtgaacgttc gagcaggcta cggcacgtag agcatcacca tgatcctgc                   49


<210>  17
<211>  64
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  misc_feature
<222>  (1)..(23)
<223>  phoshporothiate backbone between nucleotides 1-23

<220>
<221>  misc_feature
<222>  (56)..(64)
<223>  phoshporothiate backbone between nucleotides 56-64

<400>  17
tgactgtgaa cgttcgagat gacaggctac ggcacgtaga gcatcaccat gatcctgttt       60

tttt                                                                    64


<210>  18
<211>  34
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  phosphorothioate backbone between nucleotides 1-4

<220>
<221>  misc_feature
<222>  (8)..(13)
<223>  phosphorothioate backbone between nucleotides 8-13

<220>
<221>  misc_feature
<222>  (31)..(34)
<223>  phosphorothioate backbone between nucleotides 31-34

<400>  18
caggctacgt tcgtagagca tcaccatgat cctg                                   34


<210>  19
<211>  35
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  phosphorothioate backbone between nucleotides 1-4

<220>
<221>  misc_feature
<222>  (8)..(14)
<223>  phosphorothioate backbone between nucleotides 8-14

<220>
<221>  misc_feature
<222>  (32)..(35)
<223>  phosphorothioate backbone between nucleotides 32-35

<400>  19
caggctacgt ttcgtagagc atcaccatga tcctg                                  35


<210>  20
<211>  35
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  phosphorothioate backbone between nucleotides 1-4

<220>
<221>  misc_feature
<222>  (8)..(14)
<223>  phosphorothioate backbone between nucleotides 8-14

<220>
<221>  misc_feature
<222>  (32)..(35)
<223>  phosphorothioate backbone between nucleotides 32-35

<400>  20
caggcaacgt ttcgttgagc atcaccatga tcctg                                  35


<210>  21
<211>  34
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  phosphorothioate backbone between nucleotides 1-4

<220>
<221>  misc_feature
<222>  (8)..(13)
<223>  phosphorothioate backbone between nucleotides 8-13

<220>
<221>  misc_feature
<222>  (31)..(34)
<223>  phosphorothioate backbone between nucleotides 31-34

<400>  21
caggcaacgt tcgttgagca tcaccatgat cctg                                   34


<210>  22
<211>  36
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  phosphorothioate backbone between nucleotides 1-4

<220>
<221>  misc_feature
<222>  (8)..(15)
<223>  phosphorothioate backbone between nucleotides 8-15

<220>
<221>  misc_feature
<222>  (33)..(36)
<223>  phosphorothioate backbone between nucleotides 33-36

<400>  22
caggcaacgt tttcgttgag catcaccatg atcctg                                 36


<210>  23
<211>  35
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  phosphorothioate backbone between nucleotides 1-4

<220>
<221>  misc_feature
<222>  (32)..(35)
<223>  phosphorothioate backbone between nucleotides 32-35

<400>  23
caggctacgt ttcgtagagc atcaccatga tcctg                                  35


<210>  24
<211>  35
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  phosphorothioate backbone between nucleotides 1-3

<220>
<221>  misc_feature
<222>  (33)..(35)
<223>  phosphorothioate backbone between nucleotides 33-35

<400>  24
caggctacgt ttcgtagagc atcaccatga tcctg                                  35


<210>  25
<211>  36
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  phosporothioate backbone between nucleotides 1-4

<220>
<221>  misc_feature
<222>  (33)..(36)
<223>  phosporothioate backbone between nucleotides 33-36

<400>  25
caggcgtcgt tttcgacgag catcaccatg atcctg                                 36


<210>  26
<211>  36
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  phosphorothioate backbone between nucleotides 1-4

<220>
<221>  misc_feature
<222>  (33)..(36)
<223>  phosphorothioate backbone between nucleotides 33-36

<400>  26
caggcgtcgt cgtcgacgag catcaccatg atcctg                                 36


<210>  27
<211>  36
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  phosphorothioate backbone between nucleotides 1-4

<220>
<221>  misc_feature
<222>  (33)..(36)
<223>  phosphorothioate backbone between nucleotides 33-36

<400>  27
caggcttcgt cgtcgaagag catcaccatg atcctg                                 36


<210>  28
<211>  36
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  phosphorothioate backbone between nucleotides 1-4

<220>
<221>  misc_feature
<222>  (33)..(36)
<223>  phosphorothioate backbone between nucleotides 33-36

<400>  28
caggctacgt cgtcgtagag catcaccatg atcctg                                 36


<210>  29
<211>  35
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  phosphorothioate backbone between nucleotides 1-4

<220>
<221>  misc_feature
<222>  (8)..(14)
<223>  phosphorothioate backbone between nucleotides 8-14

<220>
<221>  misc_feature
<222>  (32)..(35)
<223>  phosphorothioate backbone between nucleotides 32-35

<400>  29
caggcaagct ttgcttgagc atcaccatga tcctg                                  35


<210>  30
<211>  36
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  phosphorothioate backbone between nucleotides 1-4

<220>
<221>  misc_feature
<222>  (8)..(15)
<223>  phosphorothioate backbone between nucleotides 8-15

<220>
<221>  misc_feature
<222>  (33)..(36)
<223>  phosphorothioate backbone between nucleotides 33-36

<400>  30
caggcaagct tttgcttgag catcaccatg atcctg                                 36


<210>  31
<211>  9
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  modified_base
<222>  (9)..(9)
<223>  gm

<400>  31
caggctacg                                                                9


<210>  32
<211>  12
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  modified_base
<222>  (5)..(5)
<223>  gm

<220>
<221>  modified_base
<222>  (7)..(7)
<223>  gm

<220>
<221>  modified_base
<222>  (12)..(12)
<223>  adenosine is 2'-O-Methyl

<400>  32
cgtagagcat ca                                                           12


<210>  33
<211>  8
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  modified_base
<222>  (8)..(8)
<223>  gm

<400>  33
tgatcctg                                                                 8


<210>  34
<211>  9
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  modified_base
<222>  (6)..(6)
<223>  um

<220>
<221>  modified_base
<222>  (8)..(8)
<223>  cm

<220>
<221>  modified_base
<222>  (9)..(9)
<223>  gm

<400>  34
caggcuacg                                                                9


<210>  35
<211>  12
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  modified_base
<222>  (5)..(5)
<223>  gm

<220>
<221>  modified_base
<222>  (7)..(7)
<223>  gm

<220>
<221>  modified_base
<222>  (10)..(10)
<223>  um

<220>
<221>  modified_base
<222>  (11)..(11)
<223>  cm

<220>
<221>  modified_base
<222>  (12)..(12)
<223>  adenosine is 2'-O-Methyl

<400>  35
cgtagagcau ca                                                           12


<210>  36
<211>  8
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  modified_base
<222>  (5)..(6)
<223>  cm

<220>
<221>  modified_base
<222>  (7)..(7)
<223>  um

<220>
<221>  modified_base
<222>  (8)..(8)
<223>  gm

<400>  36
tgatccug                                                                 8


<210>  37
<211>  9
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer

<400>  37
caggctacg                                                                9


<210>  38
<211>  12
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer

<400>  38
cgtagagcat ca                                                           12


<210>  39
<211>  8
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer

<400>  39
tgatcctg                                                                 8


<210>  40
<211>  9
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  modified_base
<222>  (1)..(9)
<223>  All purines (A and G) and all pyrimidines (C and U) are 
       2'-O-Methyl

<400>  40
caggcuacg                                                                9


<210>  41
<211>  12
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  modified_base
<222>  (1)..(12)
<223>  All purines (A and G) and all pyrimidines (C and U) are 
       2'-O-Methyl

<400>  41
cguagagcau ca                                                           12


<210>  42
<211>  8
<212>  RNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  modified_base
<222>  (1)..(8)
<223>  All purines (A and G) and all pyrimidines (C and U) are 
       2'-O-Methyl

<400>  42
ugauccug                                                                 8


<210>  43
<211>  10
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer

<400>  43
acaggctacg                                                              10


<210>  44
<211>  9
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer

<400>  44
tgatcctgt                                                                9


<210>  45
<211>  11
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer

<400>  45
cacaggctac g                                                            11


<210>  46
<211>  10
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer

<400>  46
tgatcctgtg                                                              10


<210>  47
<211>  9
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  cm

<220>
<221>  modified_base
<222>  (2)..(2)
<223>  adenosine is 2'-O-Methyl

<220>
<221>  modified_base
<222>  (3)..(4)
<223>  gm

<400>  47
caggctacg                                                                9


<210>  48
<211>  9
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  modified_base
<222>  (5)..(6)
<223>  cm

<220>
<221>  modified_base
<222>  (7)..(7)
<223>  um

<220>
<221>  modified_base
<222>  (8)..(8)
<223>  gm

<220>
<221>  modified_base
<222>  (9)..(9)
<223>  um

<400>  48
tgatccugu                                                                9


<210>  49
<211>  10
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  modified_base
<222>  (5)..(6)
<223>  cm

<220>
<221>  modified_base
<222>  (7)..(7)
<223>  um

<220>
<221>  modified_base
<222>  (8)..(8)
<223>  gm

<220>
<221>  modified_base
<222>  (9)..(9)
<223>  um

<220>
<221>  modified_base
<222>  (10)..(10)
<223>  gm

<400>  49
tgatccugug                                                              10


<210>  50
<211>  9
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  modified_base
<222>  (8)..(8)
<223>  cm

<220>
<221>  modified_base
<222>  (9)..(9)
<223>  gm

<400>  50
caggctacg                                                                9


<210>  51
<211>  9
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  modified_base
<222>  (6)..(6)
<223>  um

<220>
<221>  modified_base
<222>  (9)..(9)
<223>  gm

<400>  51
caggcuacg                                                                9


<210>  52
<211>  12
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  modified_base
<222>  (5)..(5)
<223>  gm

<220>
<221>  modified_base
<222>  (7)..(7)
<223>  gm

<220>
<221>  modified_base
<222>  (11)..(11)
<223>  cm

<220>
<221>  modified_base
<222>  (12)..(12)
<223>  2'-O-Methyl adenosine

<400>  52
cgtagagcat ca                                                           12


<210>  53
<211>  12
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  modified_base
<222>  (5)..(5)
<223>  gm

<220>
<221>  modified_base
<222>  (7)..(7)
<223>  gm

<220>
<221>  modified_base
<222>  (10)..(10)
<223>  um

<220>
<221>  modified_base
<222>  (12)..(12)
<223>  2'-O-Methyl adenosine

<400>  53
cgtagagcau ca                                                           12


<210>  54
<211>  9
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  modified_base
<222>  (5)..(5)
<223>  cm

<220>
<221>  modified_base
<222>  (6)..(6)
<223>  um

<220>
<221>  modified_base
<222>  (8)..(8)
<223>  cm

<400>  54
caggcuacg                                                                9


<210>  55
<211>  12
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  cm

<220>
<221>  modified_base
<222>  (3)..(3)
<223>  um

<220>
<221>  modified_base
<222>  (8)..(8)
<223>  cm

<220>
<221>  modified_base
<222>  (10)..(10)
<223>  um

<220>
<221>  modified_base
<222>  (11)..(11)
<223>  cm

<400>  55
cguagagcau ca                                                           12


<210>  56
<211>  8
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  um

<220>
<221>  modified_base
<222>  (4)..(4)
<223>  um

<220>
<221>  modified_base
<222>  (5)..(6)
<223>  cm

<220>
<221>  modified_base
<222>  (7)..(7)
<223>  um

<400>  56
ugauccug                                                                 8


<210>  57
<211>  8
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  modified_base
<222>  (6)..(6)
<223>  cm

<220>
<221>  modified_base
<222>  (7)..(7)
<223>  um

<220>
<221>  modified_base
<222>  (8)..(8)
<223>  gm

<400>  57
tgatccug                                                                 8


<210>  58
<211>  11
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  modified_base
<222>  (11)..(11)
<223>  gm

<400>  58
cacaggctac g                                                            11


<210>  59
<211>  10
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer

<400>  59
tgatcctgtg                                                              10


<210>  60
<211>  10
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  modified_base
<222>  (6)..(6)
<223>  cm

<220>
<221>  modified_base
<222>  (7)..(7)
<223>  um

<220>
<221>  modified_base
<222>  (8)..(8)
<223>  gm

<220>
<221>  modified_base
<222>  (9)..(9)
<223>  um

<220>
<221>  modified_base
<222>  (10)..(10)
<223>  gm

<400>  60
tgatccugug                                                              10


<210>  61
<211>  11
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  modified_base
<222>  (8)..(8)
<223>  um

<220>
<221>  modified_base
<222>  (10)..(10)
<223>  cm

<220>
<221>  modified_base
<222>  (11)..(11)
<223>  gm

<400>  61
cacaggcuac g                                                            11


<210>  62
<211>  8
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  modified_base
<222>  (5)..(5)
<223>  cm

<220>
<221>  modified_base
<222>  (7)..(7)
<223>  um

<220>
<221>  modified_base
<222>  (8)..(8)
<223>  gm

<400>  62
tgatccug                                                                 8


<210>  63
<211>  8
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  modified_base
<222>  (5)..(6)
<223>  cm

<220>
<221>  modified_base
<222>  (8)..(8)
<223>  gm

<400>  63
tgatcctg                                                                 8


<210>  64
<211>  11
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  modified_base
<222>  (11)..(11)
<223>  gm

<400>  64
cccaggctac g                                                            11


<210>  65
<211>  10
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  modified_base
<222>  (5)..(5)
<223>  cm

<220>
<221>  modified_base
<222>  (8)..(10)
<223>  gm

<400>  65
tgatcctggg                                                              10


<210>  66
<211>  10
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  modified_base
<222>  (8)..(10)
<223>  gm

<400>  66
tgatcctggg                                                              10


<210>  67
<211>  10
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer

<400>  67
tgatcctggg                                                              10


<210>  68
<211>  10
<212>  DNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  modified_base
<222>  (5)..(5)
<223>  cm

<400>  68
tgatcctggg                                                              10


<210>  69
<211>  10
<212>  DNA
<213>  artificial

<220>
<223>  synthetic

<400>  69
aacgttcgag                                                              10


<210>  70
<211>  87
<212>  RNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  modified_base
<222>  (1)..(87)
<223>  all pyrimidines (C and U) are 2'-fluoro

<400>  70
gggaaaagcg aaucauacac aagaucgcca ggagcaaagu cacggaggag uggggguacg       60

aaugcuccgc cagagaccaa ccgagaa                                           87


<210>  71
<211>  88
<212>  RNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  modified_base
<222>  (1)..(88)
<223>  all pyrimidines (C and U) are 2'-fluoro

<400>  71
gggaaaagcg aaucauacac aagaccggga acucggauuc uucgcaugug gaugcgauca       60

guaugcuccg ccagagacca accgagaa                                          88


<210>  72
<211>  88
<212>  RNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  modified_base
<222>  (1)..(88)
<223>  all pyrimidines (C and U)  are 2'-fluoro

<400>  72
gggaaaagcg aaucauacac aagaccggga acucggauuc uucacaugug gaugugauca       60

guaugcuccg ccagagacca accgagaa                                          88


<210>  73
<211>  88
<212>  RNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  modified_base
<222>  (1)..(88)
<223>  all pyrimidines (C and U) are 2'-fluoro

<400>  73
gggaaaagcg aaucauacac aagaccggaa acucggauuc uucgcaugug gaugcgauca       60

guaugcuccg ccagagacca accgagaa                                          88


<210>  74
<211>  88
<212>  RNA
<213>  artificial

<220>
<223>  synthetic aptamer


<220>
<221>  modified_base
<222>  (1)..(88)
<223>  all pyrimidines (C and U) are 2'-fluoro

<400>  74
gggaaaagcg aaucauacac aagagagugg aggagguaug uaugguuugu gcgucuggug       60

cggugcuccg ccagagacca accgagaa                                          88


<210>  75
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  anti-PDGF aptamer


<220>
<221>  modified_base
<222>  (30)..(30)
<223>  Inverted thymidine nucleotide that is attached to the 3' end of 
       the oligonucleotide at the 3' position on the ribose sugar

<400>  75
caggctacgc gtagagcatc atgatcctgt                                        30


<210>  76
<211>  123
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanized anti-VEGF antibody heavy chain variable domain


<220>
<221>  VARIANT
<222>  (28)..(28)
<223>  Xaa = Thr or Asp

<220>
<221>  VARIANT
<222>  (31)..(31)
<223>  Xaa = Asn or His

<220>
<221>  VARIANT
<222>  (101)..(101)
<223>  Xaa = Tyr or His

<220>
<221>  VARIANT
<222>  (105)..(105)
<223>  Xaa = Ser or Thr

<400>  76

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Tyr Xaa Phe Thr Xaa Tyr 
            20                  25                  30          


Gly Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Gly Trp Ile Asn Thr Tyr Thr Gly Glu Pro Thr Tyr Ala Ala Asp Phe 
    50                  55                  60                  


Lys Arg Arg Phe Thr Phe Ser Leu Asp Thr Ser Lys Ser Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Tyr Pro Xaa Tyr Tyr Gly Xaa Ser His Trp Tyr Phe Asp Val
            100                 105                 110         


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
        115                 120


<210>  77
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanized anti-VEGF antibody heavy chain variable domain

<400>  77

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Gly Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Gly Trp Ile Asn Thr Tyr Thr Gly Glu Pro Thr Tyr Ala Ala Asp Phe 
    50                  55                  60                  


Lys Arg Arg Phe Thr Phe Ser Leu Asp Thr Ser Lys Ser Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Tyr Pro His Tyr Tyr Gly Ser Ser His Trp Tyr Phe Asp Val 
            100                 105                 110         


Trp Gly Gln Gly Thr Leu 
        115             


<210>  78
<211>  11
<212>  PRT
<213>  Mus musculus

<400>  78

Ser Ala Ser Gln Asp Ile Ser Asn Tyr Leu Asn 
1               5                   10      


<210>  79
<211>  7
<212>  PRT
<213>  Mus musculus

<400>  79

Phe Thr Ser Ser Leu His Ser 
1               5           


<210>  80
<211>  9
<212>  PRT
<213>  Mus musculus

<400>  80

Gln Gln Tyr Ser Thr Val Pro Trp Thr 
1               5                   


<210>  81
<211>  108
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanized anti-VEGF antibody light chain variable domain


<220>
<221>  VARIANT
<222>  (4)..(4)
<223>  Xaa = Met or Leu

<400>  81

Asp Ile Gln Xaa Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Ser Ala Ser Gln Asp Ile Ser Asn Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Val Leu Ile 
        35                  40                  45              


Tyr Phe Thr Ser Ser Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Ser Thr Val Pro Trp 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg 
            100                 105             


<210>  82
<211>  110
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanized anti-VEGF antibody light chain variable domain

<400>  82

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Ser Ala Ser Gln Asp Ile Ser Asn Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Val Leu Ile 
        35                  40                  45              


Tyr Phe Thr Ser Ser Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Ser Thr Val Pro Trp 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val 
            100                 105                 110 


<210>  83
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant hypervariable region of CDRH1

<400>  83

Gly Tyr Asp Phe Thr His Tyr Gly Met Asn 
1               5                   10  


<210>  84
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant hypervariable region of CDRH3

<400>  84

Tyr Pro Tyr Tyr Tyr Gly Thr Ser His Trp Tyr Phe Asp Val 
1               5                   10                  


<210>  85
<211>  458
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VEGF-Trap - dimeric fusion polypeptide comprising two fusion 
       polypeptides

<400>  85

Met Val Ser Tyr Trp Asp Thr Gly Val Leu Leu Cys Ala Leu Leu Ser 
1               5                   10                  15      


Cys Leu Leu Leu Thr Gly Ser Ser Ser Gly Ser Asp Thr Gly Arg Pro 
            20                  25                  30          


Phe Val Glu Met Tyr Ser Glu Ile Pro Glu Ile Ile His Met Thr Glu 
        35                  40                  45              


Gly Arg Glu Leu Val Ile Pro Cys Arg Val Thr Ser Pro Asn Ile Thr 
    50                  55                  60                  


Val Thr Leu Lys Lys Phe Pro Leu Asp Thr Leu Ile Pro Asp Gly Lys 
65                  70                  75                  80  


Arg Ile Ile Trp Asp Ser Arg Lys Gly Phe Ile Ile Ser Asn Ala Thr 
                85                  90                  95      


Tyr Lys Glu Ile Gly Leu Leu Thr Cys Glu Ala Thr Val Asn Gly His 
            100                 105                 110         


Leu Tyr Lys Thr Asn Tyr Leu Thr His Arg Gln Thr Asn Thr Ile Ile 
        115                 120                 125             


Asp Val Val Leu Ser Pro Ser His Gly Ile Glu Leu Ser Val Gly Glu 
    130                 135                 140                 


Lys Leu Val Leu Asn Cys Thr Ala Arg Thr Glu Leu Asn Val Gly Ile 
145                 150                 155                 160 


Asp Phe Asn Trp Glu Tyr Pro Ser Ser Lys His Gln His Lys Lys Leu 
                165                 170                 175     


Val Asn Arg Asp Leu Lys Thr Gln Ser Gly Ser Glu Met Lys Lys Phe 
            180                 185                 190         


Leu Ser Thr Leu Thr Ile Asp Gly Val Thr Arg Ser Asp Gln Gly Leu 
        195                 200                 205             


Tyr Thr Cys Ala Ala Ser Ser Gly Leu Met Thr Lys Lys Asn Ser Thr 
    210                 215                 220                 


Phe Val Arg Val His Glu Lys Asp Lys Thr His Thr Cys Pro Pro Cys 
225                 230                 235                 240 


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
                245                 250                 255     


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
            260                 265                 270         


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
        275                 280                 285             


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
    290                 295                 300                 


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
305                 310                 315                 320 


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
                325                 330                 335     


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
            340                 345                 350         


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
        355                 360                 365             


Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
    370                 375                 380                 


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
385                 390                 395                 400 


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
                405                 410                 415     


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
            420                 425                 430         


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
        435                 440                 445             


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
    450                 455             


