                         SEQUENCE LISTING

<110>  F. Hoffmann-La Roche AG
 
<120>  Method for the selection and production of tailor-made, selective
        and multi-specific therapeutic molecules comprising at least two
        different targeting entities und uses thereof

<130>  31067 WO

<150>  EP12173878.5
<151>  2012-06-27

<160>  80    

<170>  PatentIn version 3.5

<210>  1
<211>  122
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH (mAb 1.4.168)

<400>  1

Gln Cys Asp Val Lys Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro 
1               5                   10                  15      


Gly Gly Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser 
            20                  25                  30          


Asp Tyr Pro Met Ser Trp Val Arg Gln Thr Pro Glu Lys Arg Leu Glu 
        35                  40                  45              


Trp Val Ala Thr Ile Thr Thr Gly Gly Thr Tyr Thr Tyr Tyr Pro Asp 
    50                  55                  60                  


Ser Ile Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr 
65                  70                  75                  80  


Leu Tyr Leu Gln Met Gly Ser Leu Gln Ser Glu Asp Ala Ala Met Tyr 
                85                  90                  95      


Tyr Cys Thr Arg Val Lys Thr Asp Leu Trp Trp Gly Leu Ala Tyr Trp 
            100                 105                 110         


Gly Gln Gly Thr Leu Val Thr Val Ser Ala 
        115                 120         


<210>  2
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL (mAb 1.4.168)

<400>  2

Gln Leu Val Leu Thr Gln Ser Ser Ser Ala Ser Phe Ser Leu Gly Ala 
1               5                   10                  15      


Ser Ala Lys Leu Thr Cys Thr Leu Ser Ser Gln His Ser Thr Tyr Thr 
            20                  25                  30          


Ile Glu Trp Tyr Gln Gln Gln Pro Leu Lys Pro Pro Lys Tyr Val Met 
        35                  40                  45              


Glu Leu Lys Lys Asp Gly Ser His Thr Thr Gly Asp Gly Ile Pro Asp 
    50                  55                  60                  


Arg Phe Ser Gly Ser Ser Ser Gly Ala Asp Arg Tyr Leu Ser Ile Ser 
65                  70                  75                  80  


Asn Ile Gln Pro Glu Asp Glu Ser Ile Tyr Ile Cys Gly Val Gly Asp 
                85                  90                  95      


Thr Ile Lys Glu Gln Phe Val Tyr Val Phe Gly Gly Gly Thr Lys Val 
            100                 105                 110         


Thr Val Leu Gly 
        115     


<210>  3
<211>  121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH (mAb 8.1.2)

<400>  3

Glu Val Gln Leu Gln Gln Ser Gly Pro Ala Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Phe Thr Phe Thr Ser Tyr 
            20                  25                  30          


Val Ile His Trp Val Lys Gln Lys Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Leu Asn Pro Tyr Asn Asp Asn Thr Lys Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ser Asp Arg Ser Ser Ser Thr Val Tyr 
65                  70                  75                  80  


Met Glu Phe Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Arg Gly Ile Tyr Ala Tyr Asp His Tyr Phe Asp Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Ser Leu Thr Val Ser Ser 
        115                 120     


<210>  4
<211>  106
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL (mAb 8.1.2)

<400>  4

Gln Ile Val Leu Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly 
1               5                   10                  15      


Glu Lys Val Thr Leu Thr Cys Ser Ala Ser Ser Ser Val Asn Tyr Met 
            20                  25                  30          


Tyr Trp Tyr Gln Gln Lys Pro Gly Ser Ser Pro Arg Leu Leu Ile Tyr 
        35                  40                  45              


Asp Thr Ser Asn Leu Ala Ser Gly Val Pro Val Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Val Thr Ser Tyr Ser Leu Thr Ile Ser Arg Met Glu Ala Glu 
65                  70                  75                  80  


Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Thr Tyr Pro Leu Thr 
                85                  90                  95      


Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
            100                 105     


<210>  5
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  17mer ssDNA (covalently bound with 5' end to Fab' )

<400>  5
agttctatcg tcgtcca                                                      17


<210>  6
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  19mer ssDNA (covalently bound with 3' end to Fab' )

<400>  6
agtctattaa tgcttctgc                                                    19


<210>  7
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  complementary 19mer ssDNA (used as part of a linker)

<400>  7
gcagaagcat taatagact                                                    19


<210>  8
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  complementary 17mer ssDNA (used as part of a linker)

<400>  8
tggacgacga tagaact                                                      17


<210>  9
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  fragment


<220>
<221>  MISC_FEATURE
<222>  (16)..(17)
<223>  beta-alanine

<400>  9

Glu Arg Ala Glu Gln Gln Arg Ile Arg Ala Glu Arg Glu Lys Glu Xaa 
1               5                   10                  15      


Xaa Ser Leu Lys Asp Arg Ile Glu Lys Arg Arg Arg Ala Glu Arg Ala 
            20                  25                  30          


Glu 
    


<210>  10
<211>  31
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  fragment


<220>
<221>  MISC_FEATURE
<222>  (15)..(16)
<223>  amino-trioxa-octanoic-acid

<400>  10

Leu Lys Asp Arg Ile Glu Arg Arg Arg Ala Glu Arg Ala Glu Xaa Xaa 
1               5                   10                  15      


Glu Arg Ala Glu Gln Gln Arg Ile Arg Ala Glu Arg Glu Lys Glu 
            20                  25                  30      


<210>  11
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IGF-1R (1340-1366)

<400>  11

Phe Asp Glu Arg Gln Pro Tyr Ala His Met Asn Gly Gly Arg Lys Asn 
1               5                   10                  15      


Glu Arg Ala Leu Pro Leu Pro Gln Ser Ser Thr 
            20                  25          


<210>  12
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hInsR(1355-1382)

<400>  12

Tyr Glu Glu His Ile Pro Tyr Thr His Met Asn Gly Gly Lys Lys Asn 
1               5                   10                  15      


Gly Arg Ile Leu Thr Leu Pro Arg Ser Asn Pro 
            20                  25          


<210>  13
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  35-mer polynucleotide linker


<220>
<221>  misc_feature
<222>  (1)..(35)
<223>  n is a, c, g, or t

<400>  13
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnn                                  35


<210>  14
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  75-mer polynucleotide linker


<220>
<221>  misc_feature
<222>  (1)..(75)
<223>  n is a, c, g, or t

<400>  14
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn       60

nnnnnnnnnn nnnnn                                                        75


<210>  15
<211>  95
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  95-mer polynucleotide linker


<220>
<221>  misc_feature
<222>  (1)..(95)
<223>  n is a, c, g, or t

<400>  15
nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn       60

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnn                                  95


<210>  16
<211>  218
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  4D5 Fab' heavy chain amino acid sequence

<400>  16

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn Ile Lys Asp Thr 
            20                  25                  30          


Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val 
        115                 120                 125             


Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala 
    130                 135                 140                 


Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser 
145                 150                 155                 160 


Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val 
                165                 170                 175     


Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro 
            180                 185                 190         


Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys 
        195                 200                 205             


Pro Ser Asn Thr Lys Val Asp Lys Lys Val 
    210                 215             


<210>  17
<211>  214
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  4D5 Fab' light chain amino acid sequence

<400>  17

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Asn Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr Thr Thr Pro Pro 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Gln Pro Lys 
            100                 105                 110         


Ala Ala Pro Ser Val Thr Leu Phe Pro Pro Ser Ser Glu Glu Leu Gln 
        115                 120                 125             


Ala Asn Lys Ala Thr Leu Val Cys Leu Ile Ser Asp Phe Tyr Pro Gly 
    130                 135                 140                 


Ala Val Thr Val Ala Trp Lys Ala Asp Ser Ser Pro Val Lys Ala Gly 
145                 150                 155                 160 


Val Glu Thr Thr Thr Pro Ser Lys Gln Ser Asn Asn Lys Tyr Ala Ala 
                165                 170                 175     


Ser Ser Tyr Leu Ser Leu Thr Pro Glu Gln Trp Lys Ser His Arg Ser 
            180                 185                 190         


Tyr Ser Cys Gln Val Thr His Glu Gly Ser Thr Val Glu Lys Thr Val 
        195                 200                 205             


Ala Pro Thr Glu Cys Ser 
    210                 


<210>  18
<211>  217
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2C4 Fab' heavy chain amino acid sequence

<400>  18

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Thr Asp Tyr 
            20                  25                  30          


Thr Met Asp Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Asp Val Asn Pro Asn Ser Gly Gly Ser Ile Tyr Asn Gln Arg Phe 
    50                  55                  60                  


Lys Gly Arg Phe Thr Leu Ser Val Asp Arg Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Asn Leu Gly Pro Ser Phe Tyr Phe Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe 
        115                 120                 125             


Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu 
    130                 135                 140                 


Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp 
145                 150                 155                 160 


Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu 
                165                 170                 175     


Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser 
            180                 185                 190         


Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro 
        195                 200                 205             


Ser Asn Thr Lys Val Asp Lys Lys Val 
    210                 215         


<210>  19
<211>  212
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2C4 Fab' light chain amino acid sequence

<400>  19

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asp Val Ser Ile Gly 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Tyr Arg Tyr Thr Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Tyr Ile Tyr Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Gln Pro Lys Ala Ala 
            100                 105                 110         


Pro Ser Val Thr Leu Phe Pro Pro Ser Ser Glu Glu Leu Gln Ala Asn 
        115                 120                 125             


Lys Ala Thr Leu Val Cys Leu Ile Ser Asp Phe Tyr Pro Gly Ala Val 
    130                 135                 140                 


Thr Val Ala Trp Lys Ala Asp Ser Ser Pro Val Lys Ala Gly Val Glu 
145                 150                 155                 160 


Thr Thr Thr Pro Ser Lys Gln Ser Asn Asn Lys Tyr Ala Ala Ser Ser 
                165                 170                 175     


Tyr Leu Ser Leu Thr Pro Glu Gln Trp Lys Ser His Arg Ser Tyr Ser 
            180                 185                 190         


Cys Gln Val Thr His Glu Gly Ser Thr Val Glu Lys Thr Val Ala Pro 
        195                 200                 205             


Thr Glu Cys Ser 
    210         


<210>  20
<211>  645
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HER2 ECD fragment

<400>  20

Met Glu Leu Ala Ala Leu Cys Arg Trp Gly Leu Leu Leu Ala Leu Leu 
1               5                   10                  15      


Pro Pro Gly Ala Ala Ser Thr Gln Val Cys Thr Gly Thr Asp Met Lys 
            20                  25                  30          


Leu Arg Leu Pro Ala Ser Pro Glu Thr His Leu Asp Met Leu Arg His 
        35                  40                  45              


Leu Tyr Gln Gly Cys Gln Val Val Gln Gly Asn Leu Glu Leu Thr Tyr 
    50                  55                  60                  


Leu Pro Thr Asn Ala Ser Leu Ser Phe Leu Gln Asp Ile Gln Glu Val 
65                  70                  75                  80  


Gln Gly Tyr Val Leu Ile Ala His Asn Gln Val Arg Gln Val Pro Leu 
                85                  90                  95      


Gln Arg Leu Arg Ile Val Arg Gly Thr Gln Leu Phe Glu Asp Asn Tyr 
            100                 105                 110         


Ala Leu Ala Val Leu Asp Asn Gly Asp Pro Leu Asn Asn Thr Thr Pro 
        115                 120                 125             


Val Thr Gly Ala Ser Pro Gly Gly Leu Arg Glu Leu Gln Leu Arg Ser 
    130                 135                 140                 


Leu Thr Glu Ile Leu Lys Gly Gly Val Leu Ile Gln Arg Asn Pro Gln 
145                 150                 155                 160 


Leu Cys Tyr Gln Asp Thr Ile Leu Trp Lys Asp Ile Phe His Lys Asn 
                165                 170                 175     


Asn Gln Leu Ala Leu Thr Leu Ile Asp Thr Asn Arg Ser Arg Ala Cys 
            180                 185                 190         


His Pro Cys Ser Pro Met Cys Lys Gly Ser Arg Cys Trp Gly Glu Ser 
        195                 200                 205             


Ser Glu Asp Cys Gln Ser Leu Thr Arg Thr Val Cys Ala Gly Gly Cys 
    210                 215                 220                 


Ala Arg Cys Lys Gly Pro Leu Pro Thr Asp Cys Cys His Glu Gln Cys 
225                 230                 235                 240 


Ala Ala Gly Cys Thr Gly Pro Lys His Ser Asp Cys Leu Ala Cys Leu 
                245                 250                 255     


His Phe Asn His Ser Gly Ile Cys Glu Leu His Cys Pro Ala Leu Val 
            260                 265                 270         


Thr Tyr Asn Thr Asp Thr Phe Glu Ser Met Pro Asn Pro Glu Gly Arg 
        275                 280                 285             


Tyr Thr Phe Gly Ala Ser Cys Val Thr Ala Cys Pro Tyr Asn Tyr Leu 
    290                 295                 300                 


Ser Thr Asp Val Gly Ser Cys Thr Leu Val Cys Pro Leu His Asn Gln 
305                 310                 315                 320 


Glu Val Thr Ala Glu Asp Gly Thr Gln Arg Cys Glu Lys Cys Ser Lys 
                325                 330                 335     


Pro Cys Ala Arg Val Cys Tyr Gly Leu Gly Met Glu His Leu Arg Glu 
            340                 345                 350         


Val Arg Ala Val Thr Ser Ala Asn Ile Gln Glu Phe Ala Gly Cys Lys 
        355                 360                 365             


Lys Ile Phe Gly Ser Leu Ala Phe Leu Pro Glu Ser Phe Asp Gly Asp 
    370                 375                 380                 


Pro Ala Ser Asn Thr Ala Pro Leu Gln Pro Glu Gln Leu Gln Val Phe 
385                 390                 395                 400 


Glu Thr Leu Glu Glu Ile Thr Gly Tyr Leu Tyr Ile Ser Ala Trp Pro 
                405                 410                 415     


Asp Ser Leu Pro Asp Leu Ser Val Phe Gln Asn Leu Gln Val Ile Arg 
            420                 425                 430         


Gly Arg Ile Leu His Asn Gly Ala Tyr Ser Leu Thr Leu Gln Gly Leu 
        435                 440                 445             


Gly Ile Ser Trp Leu Gly Leu Arg Ser Leu Arg Glu Leu Gly Ser Gly 
    450                 455                 460                 


Leu Ala Leu Ile His His Asn Thr His Leu Cys Phe Val His Thr Val 
465                 470                 475                 480 


Pro Trp Asp Gln Leu Phe Arg Asn Pro His Gln Ala Leu Leu His Thr 
                485                 490                 495     


Ala Asn Arg Pro Glu Asp Glu Cys Val Gly Glu Gly Leu Ala Cys His 
            500                 505                 510         


Gln Leu Cys Ala Arg Gly His Cys Trp Gly Pro Gly Pro Thr Gln Cys 
        515                 520                 525             


Val Asn Cys Ser Gln Phe Leu Arg Gly Gln Glu Cys Val Glu Glu Cys 
    530                 535                 540                 


Arg Val Leu Gln Gly Leu Pro Arg Glu Tyr Val Asn Ala Arg His Cys 
545                 550                 555                 560 


Leu Pro Cys His Pro Glu Cys Gln Pro Gln Asn Gly Ser Val Thr Cys 
                565                 570                 575     


Phe Gly Pro Glu Ala Asp Gln Cys Val Ala Cys Ala His Tyr Lys Asp 
            580                 585                 590         


Pro Pro Phe Cys Val Ala Arg Cys Pro Ser Gly Val Lys Pro Asp Leu 
        595                 600                 605             


Ser Tyr Met Pro Ile Trp Lys Phe Pro Asp Glu Glu Gly Ala Cys Gln 
    610                 615                 620                 


Pro Cys Pro Ile Asn Cys Thr His Ser Cys Val Asp Leu Asp Asp Lys 
625                 630                 635                 640 


Gly Cys Pro Ala Glu 
                645 


<210>  21
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fragment


<220>
<221>  misc_feature
<223>  X = propylene-phosphate introduced via phosphoramidite C3 
       (3-(4,4'-dimethoxytrityloxy)propyl-1-[(2-cyanoethyl)-(N,N-diisopr
       opyl)]-phosphoramidite

<220>
<221>  misc_feature
<223>  Y = 5'-amino-modifier C6 introduced via 
       (6-(4-monomethoxytritylamino)hexyl-(2-cyanoethyl)-(N,N-diisopropy
       l)-phosphoramidite

<220>
<221>  misc_feature
<223>  Z = 4[N-maleinimidomethyl]cyclohexane-1-carboxy introduced via 
       Sulfosuccinimidyl 4-[N-maleimidomethyl]cyclohexane-1-carboxylate

<220>
<221>  misc_feature
<222>  (20)..(24)
<223>  n is a, c, g, or t

<400>  21
agtctattaa tgcttctgcn nnnn                                              24


<210>  22
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  fragment


<220>
<221>  misc_feature
<223>  X = propylene-phosphate introduced via Phosphoramidite C3 
       (3-(4,4'-Dimethoxytrityloxy)propyl-1-[(2-cyanoethyl)-(N,N-diisopr
       opyl)]-phosphoramidite

<220>
<221>  misc_feature
<223>  Y = 5'-Amino-Modifier C6 introduced via 
       (6-(4-Monomethoxytritylamino)hexyl-(2-cyanoethyl)-(N,N-diisopropy
       l)-phosphoramidite

<220>
<221>  misc_feature
<223>  Z = 4[N-maleinimidomethyl]cyclohexane-1-carboxy introduced via 
       Sulfosuccinimidyl 4-[N-maleimidomethyl]cyclohexane-1-carboxylate

<220>
<221>  misc_feature
<222>  (1)..(5)
<223>  n is a, c, g, or t

<400>  22
nnnnnagttc tatcgtcgtc ca                                                22


<210>  23
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  biotinylated ssDNA linker 1


<220>
<221>  misc_feature
<223>  n=biotin dT

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  n is a, c, g, or t

<400>  23
gcagaagcat taatagactn ggacgacgat agaact                                 36


<210>  24
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  biotinylated ssDNA linker 2


<220>
<221>  misc_feature
<223>  n=biotin dT

<220>
<221>  misc_feature
<222>  (25)..(25)
<223>  n is a, c, g, or t

<400>  24
gcagaagcat taatagactt ttttnttttt ggacgacgat agaact                      46


<210>  25
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  biotinylated ssDNA linker 3


<220>
<221>  misc_feature
<223>  n=biotin dT

<220>
<221>  misc_feature
<222>  (35)..(35)
<223>  n is a, c, g, or t

<400>  25
gcagaagcat taatagactt tttttttttt ttttnttttt tttttttttt ggacgacgat       60

agaact                                                                  66


<210>  26
<211>  120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-HER2 antibody 4D5 heavy chain variable domain

<400>  26

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn Ile Lys Asp Thr 
            20                  25                  30          


Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser 
        115                 120 


<210>  27
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH CDR1

<400>  27

Gly Phe Asn Ile Lys Asp Thr Tyr Ile His 
1               5                   10  


<210>  28
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH CDR2

<400>  28

Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr Ala Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  29
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH CDR3

<400>  29

Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp Tyr 
1               5                   10      


<210>  30
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-HER2 antibody 4D5 heavy light variable domain

<400>  30

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Asn Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr Thr Thr Pro Pro 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr 
            100                 105                 


<210>  31
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL CDR1

<400>  31

Arg Ala Ser Gln Asp Val Asn Thr Ala Val Ala Trp 
1               5                   10          


<210>  32
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL CDR2

<400>  32

Ser Ala Ser Phe Leu Tyr Ser 
1               5           


<210>  33
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL CDR3

<400>  33

Gln Gln His Tyr Thr Thr Pro Pro Thr 
1               5                   


<210>  34
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-HER2 antibody 2C4 heavy chain variable domain

<400>  34

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Thr Asp Tyr 
            20                  25                  30          


Thr Met Asp Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Asp Val Asn Pro Asn Ser Gly Gly Ser Ile Tyr Asn Gln Arg Phe 
    50                  55                  60                  


Lys Gly Arg Phe Thr Leu Ser Val Asp Arg Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Asn Leu Gly Pro Ser Phe Tyr Phe Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ser 
        115                 


<210>  35
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH CDR1

<400>  35

Gly Phe Thr Phe Thr Asp Tyr Thr Met Asp 
1               5                   10  


<210>  36
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH CDR2

<400>  36

Asp Val Asn Pro Asn Ser Gly Gly Ser Ile Tyr Asn Gln Arg Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  37
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH CDR3

<400>  37

Asn Leu Gly Pro Ser Phe Tyr Phe Asp Tyr 
1               5                   10  


<210>  38
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-HER2 antibody 2C4 light chain variable domain

<400>  38

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asp Val Ser Ile Gly 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Tyr Arg Tyr Thr Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Tyr Ile Tyr Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  39
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL CDR1

<400>  39

Lys Ala Ser Gln Asp Val Ser Ile Gly Val Ala 
1               5                   10      


<210>  40
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL CDR2

<400>  40

Ser Ala Ser Tyr Arg Tyr Thr 
1               5           


<210>  41
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL CDR3

<400>  41

Gln Gln Tyr Tyr Ile Tyr Pro Tyr Thr 
1               5                   


<210>  42
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  amino modified polynucleotide linker precursors


<220>
<221>  misc_feature
<223>  X= Fluorescein Y=C7Aminolinker Z=C3 spacer

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (21)..(24)
<223>  n is a, c, g, or t

<400>  42
nagtctatta atgcttctgc nnnn                                              24


<210>  43
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  amino modified polynucleotide linker precursors


<220>
<221>  misc_feature
<223>  X=Cy5 Y=C7Aminolinker Z=C3 spacer

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (21)..(24)
<223>  n is a, c, g, or t

<400>  43
nagtctatta atgcttctgc nnnn                                              24


<210>  44
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  amino modified polynucleotide linker precursors


<220>
<221>  misc_feature
<223>  X=Aminolinker Y= Fluorescein Z=C3 spacer

<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (22)..(22)
<223>  n is a, c, g, or t

<400>  44
nnnnagttct atcgtcgtcc an                                                22


<210>  45
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  amino modified L-polynucleotide linker precursors


<220>
<221>  misc_feature
<223>  X=Fluorescein Y= C7Aminolinker Z=C3 spacer

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (21)..(24)
<223>  n is a, c, g, or t

<400>  45
nagtctatta atgcttctgc nnnn                                              24


<210>  46
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  amino modified L-polynucleotide linker precursors


<220>
<221>  misc_feature
<223>  X= Aminolinker Y=  Fluorescein Z=C3 spacer

<220>
<221>  misc_feature
<222>  (1)..(4)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (22)..(22)
<223>  n is a, c, g, or t

<400>  46
nnnnagttct atcgtcgtcc an                                                22


<210>  47
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ssDNA linker 1

<400>  47
gcagaagcat taatagactt ggacgacgat agaact                                 36


<210>  48
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ssDNA linker 2

<400>  48
gcagaagcat taatagactt tttttttttt tttttttttt tttttttttt tttttttttt       60

ggacgacgat agaact                                                       76


<210>  49
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  biotinylated ss-L-DNA linker 3


<220>
<221>  misc_feature
<223>  n=biotin dT

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  n is a, c, g, or t

<400>  49
gcagaagcat taatagactn tggacgacga tagaact                                37


<210>  50
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  biotinylated ss-L-DNA linker 4


<220>
<221>  misc_feature
<223>  n=biotin dT

<220>
<221>  misc_feature
<222>  (25)..(25)
<223>  n is a, c, g, or t

<400>  50
gcagaagcat taatagactt ttttnttttt tggacgacga tagaact                     47


<210>  51
<211>  77
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  biotinylated ss-L-DNA linker 5


<220>
<221>  misc_feature
<223>  n=biotin dT

<220>
<221>  misc_feature
<222>  (40)..(40)
<223>  n is a, c, g, or t

<400>  51
gcagaagcat taatagactt tttttttttt tttttttttn tttttttttt tttttttttt       60

tggacgacga tagaact                                                      77


<210>  52
<211>  97
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  biotinylated ss-L-DNA linker 6


<220>
<221>  misc_feature
<223>  n=biotin dT

<220>
<221>  misc_feature
<222>  (50)..(50)
<223>  n is a, c, g, or t

<400>  52
gcagaagcat taatagactt tttttttttt tttttttttt tttttttttn tttttttttt       60

tttttttttt tttttttttt tggacgacga tagaact                                97


<210>  53
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  biotinylated ssDNA linker 7


<220>
<221>  misc_feature
<223>  n=biotin dT

<220>
<221>  misc_feature
<222>  (25)..(25)
<223>  n is a, c, g, or t

<400>  53
gcagaagcat taatagactt ttttnttttt tggacgacga tagaact                     47


<210>  54
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  biotinylated ssDNA linker 8


<220>
<221>  misc_feature
<223>  n= biotin dT

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  n is a, c, g, or t

<400>  54
gcagaagcat taatagactt tttttttttn tttttttttt tggacgacga tagaact          57


<210>  55
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  biotinylated ssDNA linker 9


<220>
<221>  misc_feature
<223>  n= biotin dt

<220>
<221>  misc_feature
<222>  (35)..(35)
<223>  n is a, c, g, or t

<400>  55
gcagaagcat taatagactt tttttttttt ttttnttttt tttttttttt tggacgacga       60

tagaact                                                                 67


<210>  56
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  biotinylated ssDNA linker 10


<220>
<221>  misc_feature
<223>  n=biotin dt

<220>
<221>  misc_feature
<222>  (40)..(40)
<223>  n is a, c, g, or t

<400>  56
gcagaagcat taatagactt tttttttttt tttttttttn tttttttttt tttttttttt       60

ggacgacgat agaact                                                       76


<210>  57
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  biotinylated ssDNA linker 11


<220>
<221>  misc_feature
<223>  n=biotin dt s=spacer C18

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  n is a, c, g, or t

<400>  57
gcagaagcat taatagacts nstggacgac gatagaact                              39


<210>  58
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  biotinylated ssDNA linker 12


<220>
<221>  misc_feature
<223>  n=biotin dt s=spacer c18

<220>
<221>  misc_feature
<222>  (22)..(22)
<223>  n is a, c, g, or t

<400>  58
gcagaagcat taatagacts snsstggacg acgatagaac t                           41


<210>  59
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  biotinylated ssDNA linker 13


<220>
<221>  misc_feature
<223>  n=biotin dt s=spacer c18

<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  n is a, c, g, or t

<400>  59
gcagaagcat taatagacts ssnssstgga cgacgataga act                         43


<210>  60
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  biotinylated ss-L-DNA linker 14


<220>
<221>  misc_feature
<223>  n=biotin dt s=spacer c18

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  n is a, c, g, or t

<400>  60
gcagaagcat taatagacts sssnsssstg gacgacgata gaact                       45


<210>  61
<211>  77
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  digoxygenylated ss-L-DNA linker 15


<220>
<221>  misc_feature
<223>  n=digoxigenin dt

<220>
<221>  misc_feature
<222>  (40)..(40)
<223>  n is a, c, g, or t

<400>  61
gcagaagcat taatagactt tttttttttt tttttttttn tttttttttt tttttttttt       60

tggacgacga tagaact                                                      77


<210>  62
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  digoxygenylated ss-L-DNA linker 16


<220>
<221>  misc_feature
<223>  n=digoxigenin dt

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  n is a, c, g, or t

<400>  62
gcagaagcat taatagactn tggacgacga tagaact                                37


<210>  63
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  biotinylated ss-L-DNA linker 17


<220>
<221>  misc_feature
<223>  n=biotin dt

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  n is a, c, g, or t

<400>  63
gcagaagcat taatagactn tggacgacga tagaact                                37


<210>  64
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HA-tag

<400>  64

Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
1               5                   


<210>  65
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Avi-tag

<400>  65

Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His Glu 
1               5                   10                  15  


<210>  66
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  66

Ser Gly Gly Gly Ser 
1               5   


<210>  67
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  fMLP


<220>
<221>  MISC_FEATURE
<223>  X=f-met

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa can be any naturally occurring amino acid

<400>  67

Xaa Leu Phe 
1           


<210>  68
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  f-Met-Leu-Phe-o-methyl ester


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  f-met

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  o-methyl ester

<400>  68

Xaa Leu Phe Xaa 
1               


<210>  69
<211>  107
<212>  PRT
<213>  Homo sapiens

<400>  69

Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
1               5                   10                  15      


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
            20                  25                  30          


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
        35                  40                  45              


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
    50                  55                  60                  


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
65                  70                  75                  80  


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
                85                  90                  95      


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            100                 105         


<210>  70
<211>  105
<212>  PRT
<213>  Homo sapiens

<400>  70

Gln Pro Lys Ala Ala Pro Ser Val Thr Leu Phe Pro Pro Ser Ser Glu 
1               5                   10                  15      


Glu Leu Gln Ala Asn Lys Ala Thr Leu Val Cys Leu Ile Ser Asp Phe 
            20                  25                  30          


Tyr Pro Gly Ala Val Thr Val Ala Trp Lys Ala Asp Ser Ser Pro Val 
        35                  40                  45              


Lys Ala Gly Val Glu Thr Thr Thr Pro Ser Lys Gln Ser Asn Asn Lys 
    50                  55                  60                  


Tyr Ala Ala Ser Ser Tyr Leu Ser Leu Thr Pro Glu Gln Trp Lys Ser 
65                  70                  75                  80  


His Arg Ser Tyr Ser Cys Gln Val Thr His Glu Gly Ser Thr Val Glu 
                85                  90                  95      


Lys Thr Val Ala Pro Thr Glu Cys Ser 
            100                 105 


<210>  71
<211>  330
<212>  PRT
<213>  Homo sapiens

<400>  71

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
225                 230                 235                 240 


Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  72
<211>  330
<212>  PRT
<213>  Homo sapiens

<400>  72

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu 
225                 230                 235                 240 


Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  73
<211>  330
<212>  PRT
<213>  Homo sapiens

<400>  73

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
225                 230                 235                 240 


Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  74
<211>  327
<212>  PRT
<213>  Homo sapiens

<400>  74

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro Ala Pro 
            100                 105                 110         


Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
        115                 120                 125             


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
    130                 135                 140                 


Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp 
145                 150                 155                 160 


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe 
                165                 170                 175     


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
            180                 185                 190         


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu 
        195                 200                 205             


Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
    210                 215                 220                 


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys 
225                 230                 235                 240 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
                245                 250                 255     


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
            260                 265                 270         


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
        275                 280                 285             


Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser 
    290                 295                 300                 


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
305                 310                 315                 320 


Leu Ser Leu Ser Leu Gly Lys 
                325         


<210>  75
<211>  327
<212>  PRT
<213>  Homo sapiens

<400>  75

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro 
            100                 105                 110         


Glu Phe Glu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
        115                 120                 125             


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
    130                 135                 140                 


Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp 
145                 150                 155                 160 


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe 
                165                 170                 175     


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
            180                 185                 190         


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu 
        195                 200                 205             


Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
    210                 215                 220                 


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys 
225                 230                 235                 240 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
                245                 250                 255     


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
            260                 265                 270         


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
        275                 280                 285             


Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser 
    290                 295                 300                 


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
305                 310                 315                 320 


Leu Ser Leu Ser Leu Gly Lys 
                325         


<210>  76
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  detection probe


<220>
<221>  misc_feature
<223>  Y = 5'-Amino-Modifier Z = 3'-Aminomodifier C6 Cy5-dye at 5' and 
       3' terminus

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (22)..(23)
<223>  n is a, c, g, or t

<400>  76
nnatgcgagt accttagagt cnn                                               23


<210>  77
<211>  95
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T20-linker

<400>  77
gcagaagcat taatagactt tttttttttt tttttttttg actctaaggt actcgcattt       60

tttttttttt tttttttttg gacgacgata gaact                                  95


<210>  78
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sortase tag


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  X = any amino acid residue

<400>  78

Leu Pro Xaa Thr Gly 
1               5   


<210>  79
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligonucleotide


<220>
<221>  misc_structure
<222>  (1)..(1)
<223>  n = 5'-(Gly)4-Aminolinker-(Spacer C3)3

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n is a, c, g, or t

<220>
<221>  misc_structure
<222>  (19)..(19)
<223>  n = Fluorescein

<220>
<221>  misc_feature
<222>  (19)..(19)
<223>  n is a, c, g, or t

<400>  79
nagttctatc gtcgtccan                                                    19


<210>  80
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  L-DNA linker

<400>  80
gcagaagcat taatagactt gactctaagg tactcgcatt tttttttttt ggacgacgat       60

agaact                                                                  66


