                         SEQUENCE LISTING

<110>  Novozymes A/S
 
<120>  Rice cooking method

<130>  12424-WO-PCT

<160>  3     

<170>  PatentIn version 3.5

<210>  1
<211>  2061
<212>  DNA
<213>  Bacillus stearothermophilus


<220>
<221>  CDS
<222>  (1)..(2061)

<400>  1
agc agt tcc gca agc gtc aaa ggg gac gtg att tac cag att atc att         48
Ser Ser Ser Ala Ser Val Lys Gly Asp Val Ile Tyr Gln Ile Ile Ile           
1               5                   10                  15                

gac cgg ttt tac gat ggg gac acg acg aac aac aat cct gcc aaa agt         96
Asp Arg Phe Tyr Asp Gly Asp Thr Thr Asn Asn Asn Pro Ala Lys Ser           
            20                  25                  30                    

tat gga ctt tac gat ccg acc aaa tcg aag tgg aaa atg tat tgg ggc        144
Tyr Gly Leu Tyr Asp Pro Thr Lys Ser Lys Trp Lys Met Tyr Trp Gly           
        35                  40                  45                        

ggg gat ctg gag ggg gtt cgt caa aaa ctt cct tat ctt aaa cag ctg        192
Gly Asp Leu Glu Gly Val Arg Gln Lys Leu Pro Tyr Leu Lys Gln Leu           
    50                  55                  60                            

ggc gta acg aca atc tgg ttg tcc ccg gtt ttg gac aat ctg gat aca        240
Gly Val Thr Thr Ile Trp Leu Ser Pro Val Leu Asp Asn Leu Asp Thr           
65                  70                  75                  80            

ctg gcg ggc acc gat aac acg ggc tat cac gga tac tgg acg cgc gat        288
Leu Ala Gly Thr Asp Asn Thr Gly Tyr His Gly Tyr Trp Thr Arg Asp           
                85                  90                  95                

ttt aaa cag att gag gaa cat ttc ggg aat tgg acc aca ttt gac acg        336
Phe Lys Gln Ile Glu Glu His Phe Gly Asn Trp Thr Thr Phe Asp Thr           
            100                 105                 110                   

ttg gtc aat gat gct cac caa aac gga atc aag gtg att gtc gac ttt        384
Leu Val Asn Asp Ala His Gln Asn Gly Ile Lys Val Ile Val Asp Phe           
        115                 120                 125                       

gtg ccc aat cat tcg act cct ttt aag gca aac gat tcc acc ttt gcg        432
Val Pro Asn His Ser Thr Pro Phe Lys Ala Asn Asp Ser Thr Phe Ala           
    130                 135                 140                           

gaa ggc ggc gcc ctc tac aac aat gga acc tat atg ggc aat tat ttt        480
Glu Gly Gly Ala Leu Tyr Asn Asn Gly Thr Tyr Met Gly Asn Tyr Phe           
145                 150                 155                 160           

gat gac gca aca aaa ggg tac ttc cac cat aat ggg gac atc agc aac        528
Asp Asp Ala Thr Lys Gly Tyr Phe His His Asn Gly Asp Ile Ser Asn           
                165                 170                 175               

tgg gac gac cgg tac gag gcg caa tgg aaa aac ttc acg gat cca gcc        576
Trp Asp Asp Arg Tyr Glu Ala Gln Trp Lys Asn Phe Thr Asp Pro Ala           
            180                 185                 190                   

ggt ttc tcg ctt gcc gat ttg tcg cag gaa aat ggc acg att gct caa        624
Gly Phe Ser Leu Ala Asp Leu Ser Gln Glu Asn Gly Thr Ile Ala Gln           
        195                 200                 205                       

tac ctg acc gat gcg gcg gtt caa ttg gta gca cat gga gcg gat ggt        672
Tyr Leu Thr Asp Ala Ala Val Gln Leu Val Ala His Gly Ala Asp Gly           
    210                 215                 220                           

ttg cgg att gat gcg gtg aag cat ttt aat tcg ggg ttc tcc aaa tcg        720
Leu Arg Ile Asp Ala Val Lys His Phe Asn Ser Gly Phe Ser Lys Ser           
225                 230                 235                 240           

ttg gcc gat aaa ctg tac caa aag aaa gac att ttc ctg gtg ggg gaa        768
Leu Ala Asp Lys Leu Tyr Gln Lys Lys Asp Ile Phe Leu Val Gly Glu           
                245                 250                 255               

tgg tac gga gat gac ccc gga aca gcc aat cat ctg gaa aag gtc cgg        816
Trp Tyr Gly Asp Asp Pro Gly Thr Ala Asn His Leu Glu Lys Val Arg           
            260                 265                 270                   

tac gcc aac aac agc ggt gtc aat gtg ctg gat ttt gat ctc aac acg        864
Tyr Ala Asn Asn Ser Gly Val Asn Val Leu Asp Phe Asp Leu Asn Thr           
        275                 280                 285                       

gtg att cga aat gtg ttc ggc aca ttt acg caa acg atg tac gat ctt        912
Val Ile Arg Asn Val Phe Gly Thr Phe Thr Gln Thr Met Tyr Asp Leu           
    290                 295                 300                           

aac aat atg gtg aac caa acg ggg aac gag tac aaa tac aaa gaa aat        960
Asn Asn Met Val Asn Gln Thr Gly Asn Glu Tyr Lys Tyr Lys Glu Asn           
305                 310                 315                 320           

cta atc aca ttt atc gat aac cat gat atg tca aga ttt ctt tcg gta       1008
Leu Ile Thr Phe Ile Asp Asn His Asp Met Ser Arg Phe Leu Ser Val           
                325                 330                 335               

aat tcg aac aag gcg aat ttg cac cag gcg ctt gct ttc att ctc act       1056
Asn Ser Asn Lys Ala Asn Leu His Gln Ala Leu Ala Phe Ile Leu Thr           
            340                 345                 350                   

tcg cgg ggt acg ccc tcc atc tat tat gga acc gaa caa tac atg gca       1104
Ser Arg Gly Thr Pro Ser Ile Tyr Tyr Gly Thr Glu Gln Tyr Met Ala           
        355                 360                 365                       

ggc ggc aat gac ccg tac aac cgg ggg atg atg ccg gcg ttt gat acg       1152
Gly Gly Asn Asp Pro Tyr Asn Arg Gly Met Met Pro Ala Phe Asp Thr           
    370                 375                 380                           

aca acc acc gcc ttt aaa gag gtg tca act ctg gcg ggg ttg cgc agg       1200
Thr Thr Thr Ala Phe Lys Glu Val Ser Thr Leu Ala Gly Leu Arg Arg           
385                 390                 395                 400           

aac aat gcg gcg atc cag tac ggc acc acc acc cag cgt tgg atc aac       1248
Asn Asn Ala Ala Ile Gln Tyr Gly Thr Thr Thr Gln Arg Trp Ile Asn           
                405                 410                 415               

aat gat gtt tac att tat gaa cgg aaa ttt ttc aac gat gtc gtg ttg       1296
Asn Asp Val Tyr Ile Tyr Glu Arg Lys Phe Phe Asn Asp Val Val Leu           
            420                 425                 430                   

gtg gcc atc aat cga aac acg caa tcc tcc tat tcg att tcc ggt ttg       1344
Val Ala Ile Asn Arg Asn Thr Gln Ser Ser Tyr Ser Ile Ser Gly Leu           
        435                 440                 445                       

cag acg gcc ttg cca aat ggc agc tat gcg gat tat ctg tca ggg ctg       1392
Gln Thr Ala Leu Pro Asn Gly Ser Tyr Ala Asp Tyr Leu Ser Gly Leu           
    450                 455                 460                           

ttg ggg ggg aac ggg att tcc gtt tcc aat gga agt gtc gct tcg ttc       1440
Leu Gly Gly Asn Gly Ile Ser Val Ser Asn Gly Ser Val Ala Ser Phe           
465                 470                 475                 480           

acg ctt gcg cct gga gcc gtg tct gtt tgg cag tac agc aca tcc gct       1488
Thr Leu Ala Pro Gly Ala Val Ser Val Trp Gln Tyr Ser Thr Ser Ala           
                485                 490                 495               

tca gcg ccg caa atc gga tcg gtt gct cca aat atg ggg att ccg ggt       1536
Ser Ala Pro Gln Ile Gly Ser Val Ala Pro Asn Met Gly Ile Pro Gly           
            500                 505                 510                   

aat gtg gtc acg atc gac ggg aaa ggt ttt ggg acg acg cag gga acc       1584
Asn Val Val Thr Ile Asp Gly Lys Gly Phe Gly Thr Thr Gln Gly Thr           
        515                 520                 525                       

gtg aca ttt ggc gga gtg aca gcg act gtg aaa tcc tgg aca tcc aat       1632
Val Thr Phe Gly Gly Val Thr Ala Thr Val Lys Ser Trp Thr Ser Asn           
    530                 535                 540                           

cgg att gaa gtg tac gtt ccc aac atg gcc gcc ggg ctg acc gat gtg       1680
Arg Ile Glu Val Tyr Val Pro Asn Met Ala Ala Gly Leu Thr Asp Val           
545                 550                 555                 560           

aaa gtc acc gcg ggt gga gtt tcc agc aat ctg tat tct tac aat att       1728
Lys Val Thr Ala Gly Gly Val Ser Ser Asn Leu Tyr Ser Tyr Asn Ile           
                565                 570                 575               

ttg agt gga acg cag aca tcg gtt gtg ttt act gtg aaa agt gcg cct       1776
Leu Ser Gly Thr Gln Thr Ser Val Val Phe Thr Val Lys Ser Ala Pro           
            580                 585                 590                   

ccg acc aac ctg ggg gat aag att tac ctg acg ggc aac ata ccg gaa       1824
Pro Thr Asn Leu Gly Asp Lys Ile Tyr Leu Thr Gly Asn Ile Pro Glu           
        595                 600                 605                       

ttg ggg aat tgg agc acg gat acg agc gga gcc gtt aac aat gcg caa       1872
Leu Gly Asn Trp Ser Thr Asp Thr Ser Gly Ala Val Asn Asn Ala Gln           
    610                 615                 620                           

ggg ccc ctg ctc gcg ccc aat tat ccg gat tgg ttt tat gta ttc agc       1920
Gly Pro Leu Leu Ala Pro Asn Tyr Pro Asp Trp Phe Tyr Val Phe Ser           
625                 630                 635                 640           

gtt cca gca gga aag acg att caa ttc aag ttc ttc atc aag cgt gcg       1968
Val Pro Ala Gly Lys Thr Ile Gln Phe Lys Phe Phe Ile Lys Arg Ala           
                645                 650                 655               

gat gga acg att caa tgg gag aat ggt tcg aac cac gtg gcc aca act       2016
Asp Gly Thr Ile Gln Trp Glu Asn Gly Ser Asn His Val Ala Thr Thr           
            660                 665                 670                   

ccc acg ggt gca acc ggt aac att act gtt acg tgg caa aac tag           2061
Pro Thr Gly Ala Thr Gly Asn Ile Thr Val Thr Trp Gln Asn                   
        675                 680                 685                       


<210>  2
<211>  686
<212>  PRT
<213>  Bacillus stearothermophilus

<400>  2

Ser Ser Ser Ala Ser Val Lys Gly Asp Val Ile Tyr Gln Ile Ile Ile 
1               5                   10                  15      


Asp Arg Phe Tyr Asp Gly Asp Thr Thr Asn Asn Asn Pro Ala Lys Ser 
            20                  25                  30          


Tyr Gly Leu Tyr Asp Pro Thr Lys Ser Lys Trp Lys Met Tyr Trp Gly 
        35                  40                  45              


Gly Asp Leu Glu Gly Val Arg Gln Lys Leu Pro Tyr Leu Lys Gln Leu 
    50                  55                  60                  


Gly Val Thr Thr Ile Trp Leu Ser Pro Val Leu Asp Asn Leu Asp Thr 
65                  70                  75                  80  


Leu Ala Gly Thr Asp Asn Thr Gly Tyr His Gly Tyr Trp Thr Arg Asp 
                85                  90                  95      


Phe Lys Gln Ile Glu Glu His Phe Gly Asn Trp Thr Thr Phe Asp Thr 
            100                 105                 110         


Leu Val Asn Asp Ala His Gln Asn Gly Ile Lys Val Ile Val Asp Phe 
        115                 120                 125             


Val Pro Asn His Ser Thr Pro Phe Lys Ala Asn Asp Ser Thr Phe Ala 
    130                 135                 140                 


Glu Gly Gly Ala Leu Tyr Asn Asn Gly Thr Tyr Met Gly Asn Tyr Phe 
145                 150                 155                 160 


Asp Asp Ala Thr Lys Gly Tyr Phe His His Asn Gly Asp Ile Ser Asn 
                165                 170                 175     


Trp Asp Asp Arg Tyr Glu Ala Gln Trp Lys Asn Phe Thr Asp Pro Ala 
            180                 185                 190         


Gly Phe Ser Leu Ala Asp Leu Ser Gln Glu Asn Gly Thr Ile Ala Gln 
        195                 200                 205             


Tyr Leu Thr Asp Ala Ala Val Gln Leu Val Ala His Gly Ala Asp Gly 
    210                 215                 220                 


Leu Arg Ile Asp Ala Val Lys His Phe Asn Ser Gly Phe Ser Lys Ser 
225                 230                 235                 240 


Leu Ala Asp Lys Leu Tyr Gln Lys Lys Asp Ile Phe Leu Val Gly Glu 
                245                 250                 255     


Trp Tyr Gly Asp Asp Pro Gly Thr Ala Asn His Leu Glu Lys Val Arg 
            260                 265                 270         


Tyr Ala Asn Asn Ser Gly Val Asn Val Leu Asp Phe Asp Leu Asn Thr 
        275                 280                 285             


Val Ile Arg Asn Val Phe Gly Thr Phe Thr Gln Thr Met Tyr Asp Leu 
    290                 295                 300                 


Asn Asn Met Val Asn Gln Thr Gly Asn Glu Tyr Lys Tyr Lys Glu Asn 
305                 310                 315                 320 


Leu Ile Thr Phe Ile Asp Asn His Asp Met Ser Arg Phe Leu Ser Val 
                325                 330                 335     


Asn Ser Asn Lys Ala Asn Leu His Gln Ala Leu Ala Phe Ile Leu Thr 
            340                 345                 350         


Ser Arg Gly Thr Pro Ser Ile Tyr Tyr Gly Thr Glu Gln Tyr Met Ala 
        355                 360                 365             


Gly Gly Asn Asp Pro Tyr Asn Arg Gly Met Met Pro Ala Phe Asp Thr 
    370                 375                 380                 


Thr Thr Thr Ala Phe Lys Glu Val Ser Thr Leu Ala Gly Leu Arg Arg 
385                 390                 395                 400 


Asn Asn Ala Ala Ile Gln Tyr Gly Thr Thr Thr Gln Arg Trp Ile Asn 
                405                 410                 415     


Asn Asp Val Tyr Ile Tyr Glu Arg Lys Phe Phe Asn Asp Val Val Leu 
            420                 425                 430         


Val Ala Ile Asn Arg Asn Thr Gln Ser Ser Tyr Ser Ile Ser Gly Leu 
        435                 440                 445             


Gln Thr Ala Leu Pro Asn Gly Ser Tyr Ala Asp Tyr Leu Ser Gly Leu 
    450                 455                 460                 


Leu Gly Gly Asn Gly Ile Ser Val Ser Asn Gly Ser Val Ala Ser Phe 
465                 470                 475                 480 


Thr Leu Ala Pro Gly Ala Val Ser Val Trp Gln Tyr Ser Thr Ser Ala 
                485                 490                 495     


Ser Ala Pro Gln Ile Gly Ser Val Ala Pro Asn Met Gly Ile Pro Gly 
            500                 505                 510         


Asn Val Val Thr Ile Asp Gly Lys Gly Phe Gly Thr Thr Gln Gly Thr 
        515                 520                 525             


Val Thr Phe Gly Gly Val Thr Ala Thr Val Lys Ser Trp Thr Ser Asn 
    530                 535                 540                 


Arg Ile Glu Val Tyr Val Pro Asn Met Ala Ala Gly Leu Thr Asp Val 
545                 550                 555                 560 


Lys Val Thr Ala Gly Gly Val Ser Ser Asn Leu Tyr Ser Tyr Asn Ile 
                565                 570                 575     


Leu Ser Gly Thr Gln Thr Ser Val Val Phe Thr Val Lys Ser Ala Pro 
            580                 585                 590         


Pro Thr Asn Leu Gly Asp Lys Ile Tyr Leu Thr Gly Asn Ile Pro Glu 
        595                 600                 605             


Leu Gly Asn Trp Ser Thr Asp Thr Ser Gly Ala Val Asn Asn Ala Gln 
    610                 615                 620                 


Gly Pro Leu Leu Ala Pro Asn Tyr Pro Asp Trp Phe Tyr Val Phe Ser 
625                 630                 635                 640 


Val Pro Ala Gly Lys Thr Ile Gln Phe Lys Phe Phe Ile Lys Arg Ala 
                645                 650                 655     


Asp Gly Thr Ile Gln Trp Glu Asn Gly Ser Asn His Val Ala Thr Thr 
            660                 665                 670         


Pro Thr Gly Ala Thr Gly Asn Ile Thr Val Thr Trp Gln Asn 
        675                 680                 685     


<210>  3
<211>  621
<212>  PRT
<213>  Rhodothermus obamensis

<400>  3

Met Ser Trp Leu Thr Glu Glu Asp Ile Arg Arg Trp Glu Ser Gly Thr 
1               5                   10                  15      


Phe Tyr Asp Ser Tyr Arg Lys Leu Gly Ala His Pro Asp Asp Glu Gly 
            20                  25                  30          


Thr Trp Phe Cys Val Trp Ala Pro His Ala Asp Gly Val Ser Val Leu 
        35                  40                  45              


Gly Ala Phe Asn Asp Trp Asn Pro Glu Ala Asn Pro Leu Glu Arg Tyr 
    50                  55                  60                  


Gly Gly Gly Leu Trp Ala Gly Tyr Val Pro Gly Ala Arg Pro Gly His 
65                  70                  75                  80  


Thr Tyr Lys Tyr Arg Ile Arg His Gly Phe Tyr Gln Ala Asp Lys Thr 
                85                  90                  95      


Asp Pro Tyr Ala Phe Ala Met Glu Pro Pro Thr Gly Ser Pro Ile Glu 
            100                 105                 110         


Gly Leu Ala Ser Ile Ile Thr Arg Leu Asp Tyr Thr Trp His Asp Asp 
        115                 120                 125             


Glu Trp Met Arg Arg Arg Lys Gly Pro Ala Ser Leu Tyr Glu Pro Val 
    130                 135                 140                 


Ser Ile Tyr Glu Val His Leu Gly Ser Trp Arg His Lys Arg Pro Gly 
145                 150                 155                 160 


Glu Ser Phe Ser Tyr Arg Glu Ile Ala Glu Pro Leu Ala Asp Tyr Val 
                165                 170                 175     


Gln Glu Met Gly Phe Thr His Val Glu Leu Leu Pro Val Met Glu His 
            180                 185                 190         


Pro Tyr Tyr Gly Ser Trp Gly Tyr Gln Val Val Gly Tyr Tyr Ala Pro 
        195                 200                 205             


Thr Phe Arg Tyr Gly Ser Pro Gln Asp Leu Met Tyr Leu Ile Asp Tyr 
    210                 215                 220                 


Leu His Gln Arg Gly Ile Gly Val Ile Leu Asp Trp Val Pro Ser His 
225                 230                 235                 240 


Phe Ala Ala Asp Pro Gln Gly Leu Val Phe Phe Asp Gly Thr Thr Leu 
                245                 250                 255     


Phe Glu Tyr Asp Asp Pro Lys Met Arg Tyr His Pro Asp Trp Gly Thr 
            260                 265                 270         


Tyr Val Phe Asp Tyr Asn Lys Pro Gly Val Arg Asn Phe Leu Ile Ser 
        275                 280                 285             


Asn Ala Leu Phe Trp Leu Glu Lys Tyr His Val Asp Gly Leu Arg Val 
    290                 295                 300                 


Asp Ala Val Ala Ser Met Leu Tyr Arg Asp Tyr Ser Arg Lys Glu Trp 
305                 310                 315                 320 


Thr Pro Asn Ile Phe Gly Gly Arg Glu Asn Leu Glu Ala Ile Asp Phe 
                325                 330                 335     


Ile Lys Lys Phe Asn Glu Thr Val Tyr Leu His Phe Pro Glu Ala Met 
            340                 345                 350         


Thr Ile Ala Glu Glu Ser Thr Ala Trp Pro Gly Val Ser Ala Pro Thr 
        355                 360                 365             


Tyr Asn Asn Gly Leu Gly Phe Leu Tyr Lys Trp Asn Met Gly Trp Met 
    370                 375                 380                 


His Asp Thr Leu Asp Tyr Ile Gln Arg Asp Pro Ile Tyr Arg Lys Tyr 
385                 390                 395                 400 


His His Asp Glu Leu Thr Phe Ser Leu Trp Tyr Ala Phe Ser Glu His 
                405                 410                 415     


Tyr Val Leu Pro Leu Ser His Asp Glu Val Val His Gly Lys Gly Ser 
            420                 425                 430         


Leu Trp Gly Lys Met Pro Gly Asp Asp Trp Gln Lys Ala Ala Asn Leu 
        435                 440                 445             


Arg Leu Leu Phe Gly His Met Trp Gly His Pro Gly Lys Lys Leu Leu 
    450                 455                 460                 


Phe Met Gly Gly Glu Phe Gly Gln His His Glu Trp Asn His Asp Thr 
465                 470                 475                 480 


Gln Leu Glu Trp His Leu Leu Asp Gln Pro Tyr His Arg Gly Ile Gln 
                485                 490                 495     


Leu Trp Val Cys Asp Leu Asn His Leu Tyr Arg Thr Asn Pro Ala Leu 
            500                 505                 510         


Trp His Asp Gly Pro Glu Gly Phe Glu Trp Ile Asp Phe Ser Asp Arg 
        515                 520                 525             


Asp Gln Ser Val Ile Cys Tyr Leu Arg Lys Asn Ala Gly Arg Met Leu 
    530                 535                 540                 


Leu Phe Val Leu Asn Phe Thr Pro Val Pro Arg Glu His Tyr Arg Val 
545                 550                 555                 560 


Gly Val Pro Ile Gly Gly Pro Trp His Glu Val Leu Asn Ser Asp Ala 
                565                 570                 575     


Val Ala Tyr Gly Gly Ser Gly Met Gly Asn Phe Gly Arg Val Glu Ala 
            580                 585                 590         


Val Pro Glu Ser Trp His Gly Arg Pro Phe His Leu Glu Leu Thr Leu 
        595                 600                 605             


Pro Pro Leu Ala Ala Leu Ile Leu Glu Pro Glu His Gly 
    610                 615                 620     


