                         SEQUENCE LISTING

<110>  Research Development Foundation
 
<120>  METHOD FOR ENGINEERING PROTEASES AND PROTEIN KINASES

<130>  CLFR.P0398WO

<140>  UNKNOWN
<141>  2013-06-25

<150>  US 61/731,196
<151>  2012-11-29

<150>  US 61/720,461
<151>  2012-10-31

<150>  US 61/663,685
<151>  2012-06-25

<160>  53    

<170>  PatentIn version 3.5

<210>  1
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide

<400>  1

Glu Asn Leu Tyr Phe Glu Ser 
1               5           


<210>  2
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide

<400>  2

Glu Asn Leu Tyr Phe His Ser 
1               5           


<210>  3
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  3

Met Gln Leu Leu Arg Cys Phe Ser Ile Phe Ser Val Ile Ala Ser Val 
1               5                   10                  15      


Leu Ala 
        


<210>  4
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  4

Phe Glu His Asp Glu Leu 
1               5       


<210>  5
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  5

Lys Asp Glu Leu 
1               


<210>  6
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  6

His Asp Glu Leu 
1               


<210>  7
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  7

Arg Asp Glu Leu 
1               


<210>  8
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  8

Thr Glu Asn Leu Tyr Phe Gln Ser Gly Thr Arg Arg Trp 
1               5                   10              


<210>  9
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  9

Thr Glu Asn Leu Tyr Phe His Ser Gly Thr Arg Arg Trp 
1               5                   10              


<210>  10
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  10

Thr Glu Asn Leu Tyr Phe Glu Ser Gly Thr Arg Arg Trp 
1               5                   10              


<210>  11
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  11

Glu Asn Leu Tyr Phe Lys Ser 
1               5           


<210>  12
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is maltose-binding protein

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa is glutathione S-transferase

<400>  12

Xaa Glu Asn Leu Tyr Phe Gln Ser Xaa 
1               5                   


<210>  13
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is maltose-binding protein

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa is glutathione S-transferase

<400>  13

Xaa Glu Asn Leu Tyr Phe Glu Ser Xaa 
1               5                   


<210>  14
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is maltose-binding protein

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa is glutathione S-transferase

<400>  14

Xaa Glu Asn Leu Tyr Phe His Ser Xaa 
1               5                   


<210>  15
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is maltose-binding protein

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa is Gln, Glu, or His

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa is a polyhistidine-tag

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa is glutathione S-transferase

<400>  15

Xaa Glu Asn Leu Tyr Phe Xaa Ser Xaa Xaa 
1               5                   10  


<210>  16
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is maltose-binding protein

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa is Gln, Glu or His

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa is polyhistidine-tag

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa is TEV-P

<400>  16

Xaa Glu Asn Leu Tyr Phe Xaa Ser Xaa Xaa 
1               5                   10  


<210>  17
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  17

Asp Glu Met Glu Glu Cys Ala Ser His Leu 
1               5                   10  


<210>  18
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  18

Glu Asn Leu Tyr Phe Gln Ser 
1               5           


<210>  19
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  19

Cys Gly Tyr Gly Pro Lys Lys Lys Arg Lys Val Gly Gly 
1               5                   10              


<210>  20
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide


<220>
<221>  MISC_FEATURE
<222>  (2)..(3)
<223>  Xaa can be any naturally occuring amino acid

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa can be any naturally occuring amino acid

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa is Gly or Ser

<400>  20

Glu Xaa Xaa Tyr Xaa Gln Xaa 
1               5           


<210>  21
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  21

Glu Asn Leu Tyr Phe Gln Gly 
1               5           


<210>  22
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  22

Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


<210>  23
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa is any residue

<400>  23

Glu Asn Leu Tyr Phe Xaa Ser 
1               5           


<210>  24
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  24

His His His His His His 
1               5       


<210>  25
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  25

Gln Leu Leu Arg Cys Phe Ser Ile Phe Ser Val Ile Ala Ser Val Leu 
1               5                   10                  15      


Ala 
    


<210>  26
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  26

Lys Glu Glu Leu 
1               


<210>  27
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide


<220>
<221>  misc_feature
<222>  (3)..(4)
<223>  Xaa can be any naturally occurring amino acid

<400>  27

Lys Lys Xaa Xaa 
1               


<210>  28
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  28

Val Glu Lys Pro Phe Ala Ile Ala Lys Glu 
1               5                   10  


<210>  29
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  29

Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu 
1               5                   10  


<210>  30
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  30

Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
1               5                   


<210>  31
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  31

Tyr Thr Asp Ile Glu Met Asn Arg Leu Gly Lys 
1               5                   10      


<210>  32
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  32

Gln Pro Glu Leu Ala Pro Glu Asp Pro Glu Asp 
1               5                   10      


<210>  33
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  33

Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr 
1               5                   10                  


<210>  34
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  34

Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


<210>  35
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer


<220>
<221>  misc_feature
<222>  (18)..(19)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (24)..(25)
<223>  n is a, c, g, or t

<400>  35
ggaagcattg gattcaanns aagnnsgggc agtgtggcag tcc                         43


<210>  36
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  36
attagtatca actagagatg ggttcattgt tggtata                                37


<210>  37
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  37
atctctagtt gatactaatg gactgccaca ctgccc                                 36


<210>  38
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer


<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (28)..(29)
<223>  n is a, c, g, or t

<400>  38
tttgtgttgg tgaaattsnn tgctgasnnt ataccaacaa tgaaccc                     47


<210>  39
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  39
ttgaatccaa tgcttccaga a                                                 21


<210>  40
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  40
aatttcacca acacaaacaa                                                   20


<210>  41
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  41
taccatctgc agagcgacgg cgacgacgat tcatgag                                37


<210>  42
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  42
atggttggta ccgaaaatct ttattttagc ggtcatcatc atc                         43


<210>  43
<211>  136
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  43
cgtcaaggag aaaaaacccc ggatccgtaa tacgactcac tatagggccc gggcgtcgac       60

atgcaacttt tgagatgctt cagtattttc agcgtcatcg ccagtgtgct ggccagcttg      120

tttaaggggc cgcgtg                                                      136


<210>  44
<211>  157
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  44
gtacagtggg aacaaagtcg attttgttac atctacactg ttgttatcag atctcgagcg       60

gtaccttact cattacaatt cgtcgtgttc gaaactaccc aagtcctctt cagaaataag      120

cttttgttcg gatccattca tgagttgagt cgcttcc                               157


<210>  45
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  45
atggctggcc cagccggcca gcttgtttaa ggggccgcg                              39


<210>  46
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  46
gtccatggcc cccgaggcct taattcatga gttgagtcgc ttccttaac                   49


<210>  47
<211>  97
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  47
gtacagtggg aacaaagtcg attttgttac atctacactg ttgttatcag atctcgagcg       60

gtaccttact cattaattca tgagttgagt cgcttcc                                97


<210>  48
<211>  106
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer


<220>
<221>  misc_feature
<222>  (60)..(61)
<223>  n is a, c, g, or t

<400>  48
gagctcacaa ttcgtcgtgt tcgaaactac catgatgatg atgatgatga ctgccagasn       60

ngaaatacaa attttcactg cctttatcgt cgtcatcttt ataatc                     106


<210>  49
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  49
cgaattcaac cctcactaaa gggcggccgc actagtatcg atg                         43


<210>  50
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  50

Glu Asn Leu Tyr Glu Ser 
1               5       


<210>  51
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  51

Ser Gly Thr Arg Arg Trp 
1               5       


<210>  52
<211>  234
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  52

Ser Leu Phe Lys Gly Pro Arg Asp Tyr Asn Pro Ile Ser Ser Thr Ile 
1               5                   10                  15      


Cys His Leu Thr Asn Glu Ser Asp Gly His Thr Thr Ser Leu Tyr Gly 
            20                  25                  30          


Ile Gly Phe Gly Pro Phe Ile Ile Thr Asn Lys His Leu Phe Arg Arg 
        35                  40                  45              


Asn Asn Gly Thr Leu Leu Val Gln Ser Leu His Gly Val Phe Lys Val 
    50                  55                  60                  


Lys Asn Thr Thr Thr Leu Gln Gln His Leu Ile Asp Gly Arg Asp Met 
65                  70                  75                  80  


Ile Ile Ile Arg Met Pro Lys Asp Phe Pro Pro Phe Pro Gln Lys Leu 
                85                  90                  95      


Lys Phe Arg Glu Pro Gln Arg Glu Glu Arg Ile Cys Leu Val Thr Thr 
            100                 105                 110         


Asn Phe Gln Thr Lys Ser Met Ser Ser Met Val Ser Asp Thr Ser Cys 
        115                 120                 125             


Thr Phe Pro Ser Ser Asp Gly Ile Phe Trp Lys His Trp Ile Gln Thr 
    130                 135                 140                 


Lys Asp Gly Gln Cys Gly Ser Pro Leu Val Ser Thr Arg Asp Gly Phe 
145                 150                 155                 160 


Ile Val Gly Ile His Ser Ala Ser Asn Phe Thr Asn Thr Asn Asn Tyr 
                165                 170                 175     


Phe Thr Ser Val Pro Lys Asn Phe Met Glu Leu Leu Thr Asn Gln Glu 
            180                 185                 190         


Ala Gln Gln Trp Val Ser Gly Trp Arg Leu Asn Ala Asp Ser Val Leu 
        195                 200                 205             


Trp Gly Gly His Lys Val Phe Met Pro Lys Pro Glu Glu Pro Phe Gln 
    210                 215                 220                 


Pro Val Lys Glu Ala Thr Gln Leu Met Asn 
225                 230                 


<210>  53
<211>  234
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  53

Ser Leu Phe Lys Gly Pro Arg Asp Tyr Asn Pro Ile Ser Ser Thr Ile 
1               5                   10                  15      


Cys His Leu Thr Asn Glu Ser Asp Gly His Thr Thr Ser Leu Tyr Gly 
            20                  25                  30          


Ile Gly Phe Gly Pro Phe Ile Ile Thr Asn Lys His Leu Phe Arg Arg 
        35                  40                  45              


Asn Asn Gly Thr Leu Leu Val Gln Ser Leu His Gly Val Phe Lys Val 
    50                  55                  60                  


Lys Asn Thr Thr Thr Leu Gln Gln His Leu Ile Asp Gly Arg Asp Met 
65                  70                  75                  80  


Ile Ile Ile Arg Met Pro Lys Asp Phe Pro Pro Phe Pro Gln Lys Leu 
                85                  90                  95      


Lys Phe Arg Glu Pro Gln Arg Glu Glu Arg Ile Cys Leu Val Thr Thr 
            100                 105                 110         


Asn Phe Gln Thr Lys Arg Met Ser Ser Met Val Ser Asp Thr Ser Cys 
        115                 120                 125             


Thr Phe Pro Ser Ser Asp Gly Ile Phe Trp Lys His Trp Ile Gln Thr 
    130                 135                 140                 


Lys Arg Gly Gln Cys Gly Ser Pro Leu Val Ser Thr Arg Asp Gly Phe 
145                 150                 155                 160 


Ile Val Gly Ile His Ser Ala Ser Asn Phe Ala Asn Thr Asn Lys Tyr 
                165                 170                 175     


Phe Thr Ser Val Pro Lys Asn Phe Met Glu Leu Leu Thr Asn Gln Glu 
            180                 185                 190         


Ala Gln Gln Trp Val Ser Gly Trp Arg Leu Asn Ala Asp Ser Val Leu 
        195                 200                 205             


Trp Gly Gly His Lys Val Phe Ile Pro Lys Pro Glu Glu Pro Phe Gln 
    210                 215                 220                 


Pro Val Lys Glu Ala Thr Gln Leu Met Asn 
225                 230                 


