                         SEQUENCE LISTING

<110>  Grawunder, Ulf
 
<120>  Transposition-mediated identification of specific binding or 
       functional proteins

<130>  ND 40268

<160>  55    

<170>  PatentIn version 3.5

<210>  1
<211>  327
<212>  DNA
<213>  Artificial sequence

<220>
<223>  PiggyBac ITR sequence

<400>  1
atatcaattg ttaaccctag aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa       60

tattgctctc tctttctaaa tagcgcgaat ccgtcgctgt gcatttagga catctcagtc      120

gccgcttgga gctcccgtga ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac      180

tataacgacc gcgtgagtca aaatgacgca tgattatctt ttacgtgact tttaagattt      240

aactcatacg ataattatat tgttatttca tgttctactt acgtgataac ttattatata      300

tatattttct tgttatacaa ttgatat                                          327


<210>  2
<211>  264
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  downstream PiggyBac ITR sequence

<400>  2
atatctcgag ttaaccctag aaagataatc atattgtgac gtacgttaaa gataatcatg       60

cgtaaaattg acgcatgtgt tttatcggtc tgtatatcga ggtttattta ttaatttgaa      120

tagatattaa gttttattat atttacactt acatactaat aataaattca acaaacaatt      180

tatttatgtt tatttattta ttaaaaaaaa acaaaaactc aaaatttctt ctataaagta      240

acaaaacttt tatcctcgag atat                                             264


<210>  3
<211>  711
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  V region of D2E7

<400>  3
atggacatga gggtccctgc tcagctcctg ggactcctgc tgctctggct cccaggtgcc       60

agatgtgaca tccagatgac ccagtctcca tcctccctgt ctgcatctgt aggggacaga      120

gtcaccatca cttgtcgggc aagtcagggc atcagaaatt acttagcctg gtatcagcaa      180

aaaccaggga aagcccctaa gctcctgatc tatgctgcat ccactttgca atcaggggtc      240

ccatctcggt tcagtggcag tggatctggg acagatttca ctctcaccat cagcagccta      300

cagcctgaag atgttgcaac ttattactgt caaaggtata accgtgcacc gtatactttt      360

ggccagggga ccaaggtgga aatcaagcgc tctgtggctg caccatctgt cttcatcttc      420

ccgccatctg atgagcagtt gaaatctgga actgcctctg ttgtgtgcct gctgaataac      480

ttctatccca gagaggccaa agtacagtgg aaggtggata acgccctcca atcgggtaac      540

tcccaggaga gtgtcacaga gcaggacagc aaggacagca cctacagcct cagcagcacc      600

ctgacgctga gcaaagcaga ctacgagaaa cacaaagtct acgcctgcga agtcacccat      660

cagggcctga gctcgcccgt cacaaagagc ttcaacaggg gagagtgtta a               711


<210>  4
<211>  236
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein sequence of V region of D2E7

<400>  4

Met Asp Met Arg Val Pro Ala Gln Leu Leu Gly Leu Leu Leu Leu Trp 
1               5                   10                  15      


Leu Pro Gly Ala Arg Cys Asp Ile Gln Met Thr Gln Ser Pro Ser Ser 
            20                  25                  30          


Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser 
        35                  40                  45              


Gln Gly Ile Arg Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys 
    50                  55                  60                  


Ala Pro Lys Leu Leu Ile Tyr Ala Ala Ser Thr Leu Gln Ser Gly Val 
65                  70                  75                  80  


Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
                85                  90                  95      


Ile Ser Ser Leu Gln Pro Glu Asp Val Ala Thr Tyr Tyr Cys Gln Arg 
            100                 105                 110         


Tyr Asn Arg Ala Pro Tyr Thr Phe Gly Gln Gly Thr Lys Val Glu Ile 
        115                 120                 125             


Lys Arg Ser Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp 
    130                 135                 140                 


Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn 
145                 150                 155                 160 


Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu 
                165                 170                 175     


Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp 
            180                 185                 190         


Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr 
        195                 200                 205             


Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser 
    210                 215                 220                 


Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
225                 230                 235     


<210>  5
<211>  6436
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pIRES-EGFP-T1T2-IgL

<400>  5
gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg       60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg      120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgttaa ccctagaaag      180

atagtctgcg taaaattgac gcatgcattc ttgaaatatt gctctctctt tctaaatagc      240

gcgaatccgt cgctgtgcat ttaggacatc tcagtcgccg cttggagctc ccgtgaggcg      300

tgcttgtcaa tgcggtaagt gtcactgatt ttgaactata acgaccgcgt gagtcaaaat      360

gacgcatgat tatcttttac gtgactttta agatttaact catacgataa ttatattgtt      420

atttcatgtt ctacttacgt gataacttat tatatatata ttttcttgtt atacaattgc      480

atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat      540

acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt      600

catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga      660

ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca      720

atagggactt tccattgacg tcaatgggtg gactatttac ggtaaactgc ccacttggca      780

gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg      840

cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc      900

tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt      960

ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt     1020

ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg     1080

acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta     1140

actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc     1200

caagcttggt accgagctcg gatcgatatg gacatgaggg tccctgctca gctcctggga     1260

ctcctgctgc tctggctccc aggtgccaga tgtgacatcc agatgaccca gtctccatcc     1320

tccctgtctg catctgtagg ggacagagtc accatcactt gtcgggcaag tcagggcatc     1380

agaaattact tagcctggta tcagcaaaaa ccagggaaag cccctaagct cctgatctat     1440

gctgcatcca ctttgcaatc aggggtccca tctcggttca gtggcagtgg atctgggaca     1500

gatttcactc tcaccatcag cagcctacag cctgaagatg ttgcaactta ttactgtcaa     1560

aggtataacc gtgcaccgta tacttttggc caggggacca aggtggaaat caagcgctct     1620

gtggctgcac catctgtctt catcttcccg ccatctgatg agcagttgaa atctggaact     1680

gcctctgttg tgtgcctgct gaataacttc tatcccagag aggccaaagt acagtggaag     1740

gtggataacg ccctccaatc gggtaactcc caggagagtg tcacagagca ggacagcaag     1800

gacagcacct acagcctcag cagcaccctg acgctgagca aagcagacta cgagaaacac     1860

aaagtctacg cctgcgaagt cacccatcag ggcctgagct cgcccgtcac aaagagcttc     1920

aacaggggag agtgttaaat ctgcggccgc gtcgacggaa ttcagtggat ccactagtaa     1980

cggccgccag tgtgctggaa ttaattcgct gtctgcgagg gccagctgtt ggggtgagta     2040

ctccctctca aaagcgggca tgacttctgc gctaagattg tcagtttcca aaaacgagga     2100

ggatttgata ttcacctggc ccgcggtgat gcctttgagg gtggccgcgt ccatctggtc     2160

agaaaagaca atctttttgt tgtcaagctt gaggtgtggc aggcttgaga tctggccata     2220

cacttgagtg acaatgacat ccactttgcc tttctctcca caggtgtcca ctcccaggtc     2280

caactgcagg tcgagcatgc atctagggcg gccaattccg cccctctccc tccccccccc     2340

ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgtgat     2400

tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct     2460

tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg     2520

tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc     2580

tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg     2640

tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg     2700

tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga     2760

aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt     2820

agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa     2880

aaacacgatg ataagcttgc cacaacccgg gatccaccgg tcgccaccat ggtgagcaag     2940

ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac     3000

ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc     3060

ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc     3120

ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc     3180

ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac     3240

ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc     3300

gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac     3360

aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg     3420

aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag     3480

cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc     3540

cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc     3600

gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggccctaga     3660

gctcgctgat cagcctcgac tgtgcctcta gttgccagcc atctgttgtt tgcccctccc     3720

ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg     3780

aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg     3840

acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta     3900

tggcttctga ggcggaaaga accagctggg gctcgaggat aaaagttttg ttactttata     3960

gaagaaattt tgagtttttg ttttttttta ataaataaat aaacataaat aaattgtttg     4020

ttgaatttat tattagtatg taagtgtaaa tataataaaa cttaatatct attcaaatta     4080

ataaataaac ctcgatatac agaccgataa aacacatgcg tcaattttac gcatgattat     4140

ctttaacgta cgtcacaata tgattatctt tctagggtta actcgagtgc attctagttg     4200

tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta     4260

gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat     4320

tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag     4380

ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg     4440

ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc     4500

ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc     4560

agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa     4620

catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt     4680

tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg     4740

gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg     4800

ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag     4860

cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc     4920

caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa     4980

ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg     5040

taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc     5100

taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac     5160

cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg     5220

tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt     5280

gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt     5340

catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa     5400

atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga     5460

ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt     5520

gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg     5580

agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga     5640

gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga     5700

agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg     5760

catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc     5820

aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc     5880

gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca     5940

taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac     6000

caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg     6060

ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc     6120

ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg     6180

tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac     6240

aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat     6300

actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata     6360

catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa     6420

agtgccacct gacgtc                                                     6436


<210>  6
<211>  1642
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VH region of D2E7 with restriction sites

<400>  6
aattatcgat atggagtttg ggctgagctg ggttttcctt gttgcgattt tagaaggtgt       60

ccagtgtgag gtgcagctgg tggagtctgg gggaggcttg gtacagcccg gcaggtccct      120

gagactctcc tgtgcggcct ctggattcac ctttgatgat tatgccatgc actgggtccg      180

gcaagctcca gggaagggcc tggaatgggt ctcagctatc acttggaata gtggtcacat      240

agactatgcg gactctgtgg agggccgatt caccatctcc agagacaacg ccaagaactc      300

cctgtatctg caaatgaaca gtctgagagc tgaggatacg gccgtatatt actgtgcgaa      360

agtctcgtac cttagcaccg cgtcctccct tgactattgg ggccaaggta ccctggtcac      420

cgtctcgagc gcttccacca agggcccatc ggtcttcccc ctggcaccct cctccaagag      480

cacctctggg ggcacagcag ccctgggctg cctggtcaag gactacttcc ccgaaccggt      540

gacggtgtcg tggaactcag gcgccctgac cagcggcgtg cacaccttcc cggctgtcct      600

acagtcctca ggactctact ccctcagcag cgtggtgacc gtgccctcca gcagcttggg      660

cacccagacc tacatctgca acgtgaatca caagcccagc aacaccaagg tggacaagaa      720

agttgagccc aaatcttgtg acaaaactca cacatgccca ccgtgcccag cacctgaact      780

cctgggggga ccgtcagtct tcctcttccc cccaaaaccc aaggacaccc tcatgatctc      840

ccggacccct gaggtcacat gcgtggtggt ggacgtgagc cacgaagacc ctgaggtcaa      900

gttcaactgg tacgtggacg gcgtggaggt gcataatgcc aagacaaagc cgcgggagga      960

gcagtacaac agcacgtacc gtgtggtcag cgtcctcacc gtcctgcacc aggactggct     1020

gaatggcaag gagtacaagt gcaaggtctc caacaaagcc ctcccagccc ccatcgagaa     1080

aaccatctcc aaagccaaag ggcagccccg agaaccacag gtgtacaccc tgcccccatc     1140

ccgggatgag ctgaccaaga accaggtcag cctgacctgc ctggtcaaag gcttctatcc     1200

cagcgacatc gccgtggagt gggagagcaa tgggcagccg gagaacaact acaagaccac     1260

gcctcccgtg ctggactccg acggctcctt cttcctctac agcaagctca ccgtggacaa     1320

gagcaggtgg cagcagggga acgtcttctc atgctccgtg atgcatgagg ctctgcacaa     1380

ccactacaca cagaagagcc tctccctgtc tccggagctg caactggagg agagctgtgc     1440

ggaggcgcag gacggggagc tggacgggct gtggacgacc atcaccatct tcatcacact     1500

cttcctgtta agcgtgtgct acagtgccac cgtcaccttc ttcaaggtga agtggatctt     1560

ctcctcggtg gtggacctga agcagaccat catccccgac tacaggaaca tgatcggaca     1620

gggggcctag gcggccgcgt cg                                              1642


<210>  7
<211>  539
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein sequence translated from Seq ID No 6

<400>  7

Met Glu Phe Gly Leu Ser Trp Val Phe Leu Val Ala Ile Leu Glu Gly 
1               5                   10                  15      


Val Gln Cys Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln 
            20                  25                  30          


Pro Gly Arg Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe 
        35                  40                  45              


Asp Asp Tyr Ala Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
    50                  55                  60                  


Glu Trp Val Ser Ala Ile Thr Trp Asn Ser Gly His Ile Asp Tyr Ala 
65                  70                  75                  80  


Asp Ser Val Glu Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn 
                85                  90                  95      


Ser Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Lys Val Ser Tyr Leu Ser Thr Ala Ser Ser Leu Asp 
        115                 120                 125             


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys 
    130                 135                 140                 


Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly 
145                 150                 155                 160 


Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro 
                165                 170                 175     


Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr 
            180                 185                 190         


Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val 
        195                 200                 205             


Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn 
    210                 215                 220                 


Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro 
225                 230                 235                 240 


Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu 
                245                 250                 255     


Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 
            260                 265                 270         


Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp 
        275                 280                 285             


Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly 
    290                 295                 300                 


Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn 
305                 310                 315                 320 


Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp 
                325                 330                 335     


Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro 
            340                 345                 350         


Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu 
        355                 360                 365             


Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn 
    370                 375                 380                 


Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 
385                 390                 395                 400 


Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 
                405                 410                 415     


Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 
            420                 425                 430         


Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 
        435                 440                 445             


Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu 
    450                 455                 460                 


Ser Leu Ser Pro Glu Leu Gln Leu Glu Glu Ser Cys Ala Glu Ala Gln 
465                 470                 475                 480 


Asp Gly Glu Leu Asp Gly Leu Trp Thr Thr Ile Thr Ile Phe Ile Thr 
                485                 490                 495     


Leu Phe Leu Leu Ser Val Cys Tyr Ser Ala Thr Val Thr Phe Phe Lys 
            500                 505                 510         


Val Lys Trp Ile Phe Ser Ser Val Val Asp Leu Lys Gln Thr Ile Ile 
        515                 520                 525             


Pro Asp Tyr Arg Asn Met Ile Gly Gln Gly Ala 
    530                 535                 


<210>  8
<211>  7341
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence for the transposable human antibody gamma-1 heavy chain 
       vector pIRES-EGFP- T1T2-IgH

<400>  8
gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg       60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg      120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgttaa ccctagaaag      180

atagtctgcg taaaattgac gcatgcattc ttgaaatatt gctctctctt tctaaatagc      240

gcgaatccgt cgctgtgcat ttaggacatc tcagtcgccg cttggagctc ccgtgaggcg      300

tgcttgtcaa tgcggtaagt gtcactgatt ttgaactata acgaccgcgt gagtcaaaat      360

gacgcatgat tatcttttac gtgactttta agatttaact catacgataa ttatattgtt      420

atttcatgtt ctacttacgt gataacttat tatatatata ttttcttgtt atacaattgc      480

atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat      540

acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt      600

catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga      660

ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca      720

atagggactt tccattgacg tcaatgggtg gactatttac ggtaaactgc ccacttggca      780

gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg      840

cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc      900

tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt      960

ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt     1020

ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg     1080

acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta     1140

actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc     1200

caagcttggt accgagctcg gatcgatatg gagtttgggc tgagctgggt tttccttgtt     1260

gcgattttag aaggtgtcca gtgtgaggtg cagctggtgg agtctggggg aggcttggta     1320

cagcccggca ggtccctgag actctcctgt gcggcctctg gattcacctt tgatgattat     1380

gccatgcact gggtccggca agctccaggg aagggcctgg aatgggtctc agctatcact     1440

tggaatagtg gtcacataga ctatgcggac tctgtggagg gccgattcac catctccaga     1500

gacaacgcca agaactccct gtatctgcaa atgaacagtc tgagagctga ggatacggcc     1560

gtatattact gtgcgaaagt ctcgtacctt agcaccgcgt cctcccttga ctattggggc     1620

caaggtaccc tggtcaccgt ctcgagcgct tccaccaagg gcccatcggt cttccccctg     1680

gcaccctcct ccaagagcac ctctgggggc acagcagccc tgggctgcct ggtcaaggac     1740

tacttccccg aaccggtgac ggtgtcgtgg aactcaggcg ccctgaccag cggcgtgcac     1800

accttcccgg ctgtcctaca gtcctcagga ctctactccc tcagcagcgt ggtgaccgtg     1860

ccctccagca gcttgggcac ccagacctac atctgcaacg tgaatcacaa gcccagcaac     1920

accaaggtgg acaagaaagt tgagcccaaa tcttgtgaca aaactcacac atgcccaccg     1980

tgcccagcac ctgaactcct ggggggaccg tcagtcttcc tcttcccccc aaaacccaag     2040

gacaccctca tgatctcccg gacccctgag gtcacatgcg tggtggtgga cgtgagccac     2100

gaagaccctg aggtcaagtt caactggtac gtggacggcg tggaggtgca taatgccaag     2160

acaaagccgc gggaggagca gtacaacagc acgtaccgtg tggtcagcgt cctcaccgtc     2220

ctgcaccagg actggctgaa tggcaaggag tacaagtgca aggtctccaa caaagccctc     2280

ccagccccca tcgagaaaac catctccaaa gccaaagggc agccccgaga accacaggtg     2340

tacaccctgc ccccatcccg ggatgagctg accaagaacc aggtcagcct gacctgcctg     2400

gtcaaaggct tctatcccag cgacatcgcc gtggagtggg agagcaatgg gcagccggag     2460

aacaactaca agaccacgcc tcccgtgctg gactccgacg gctccttctt cctctacagc     2520

aagctcaccg tggacaagag caggtggcag caggggaacg tcttctcatg ctccgtgatg     2580

catgaggctc tgcacaacca ctacacacag aagagcctct ccctgtctcc ggagctgcaa     2640

ctggaggaga gctgtgcgga ggcgcaggac ggggagctgg acgggctgtg gacgaccatc     2700

accatcttca tcacactctt cctgttaagc gtgtgctaca gtgccaccgt caccttcttc     2760

aaggtgaagt ggatcttctc ctcggtggtg gacctgaagc agaccatcat ccccgactac     2820

aggaacatga tcggacaggg ggcctaggcg gccgcgtcga cggaattcag tggatccact     2880

agtaacggcc gccagtgtgc tggaattaat tcgctgtctg cgagggccag ctgttggggt     2940

gagtactccc tctcaaaagc gggcatgact tctgcgctaa gattgtcagt ttccaaaaac     3000

gaggaggatt tgatattcac ctggcccgcg gtgatgcctt tgagggtggc cgcgtccatc     3060

tggtcagaaa agacaatctt tttgttgtca agcttgaggt gtggcaggct tgagatctgg     3120

ccatacactt gagtgacaat gacatccact ttgcctttct ctccacaggt gtccactccc     3180

aggtccaact gcaggtcgag catgcatcta gggcggccaa ttccgcccct ctccctcccc     3240

cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat     3300

gtgattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt     3360

cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt     3420

gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc     3480

gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc     3540

acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat     3600

agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc     3660

ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg     3720

tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct     3780

ttgaaaaaca cgatgataag cttgccacaa cccgggatcc accggtcgcc accatggtga     3840

gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg     3900

taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc     3960

tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga     4020

ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg     4080

acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg     4140

acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc     4200

gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg     4260

agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca     4320

aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact     4380

accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga     4440

gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg     4500

agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taaagcggcc     4560

ctagagctcg ctgatcagcc tcgactgtgc ctctagttgc cagccatctg ttgtttgccc     4620

ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa     4680

tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg     4740

gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg     4800

ctctatggct tctgaggcgg aaagaaccag ctggggctcg aggataaaag ttttgttact     4860

ttatagaaga aattttgagt ttttgttttt ttttaataaa taaataaaca taaataaatt     4920

gtttgttgaa tttattatta gtatgtaagt gtaaatataa taaaacttaa tatctattca     4980

aattaataaa taaacctcga tatacagacc gataaaacac atgcgtcaat tttacgcatg     5040

attatcttta acgtacgtca caatatgatt atctttctag ggttaactcg agtgcattct     5100

agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct     5160

agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc     5220

acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga     5280

gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg     5340

tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg     5400

cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg     5460

gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga     5520

aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg     5580

gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag     5640

aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc     5700

gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg     5760

ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt     5820

cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc     5880

ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc     5940

actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg     6000

tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca     6060

gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc     6120

ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat     6180

cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt     6240

ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt     6300

tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc     6360

agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc     6420

gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata     6480

ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg     6540

gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc     6600

cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct     6660

acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa     6720

cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt     6780

cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca     6840

ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac     6900

tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca     6960

atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt     7020

tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc     7080

actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca     7140

aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata     7200

ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc     7260

ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc     7320

cgaaaagtgc cacctgacgt c                                               7341


<210>  9
<211>  437
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  libraries for variable heavy chain domains containing N-sequences
       in the HCDR3 and flanked by ClaI and Eco47III restriction sites


<220>
<221>  misc_feature
<222>  (362)..(385)
<223>  N = wildcard, nucleotide can be any of the four nucleotides 
       occuring in DNA

<400>  9
aattatcgat atggagtttg ggctgagctg ggttttcctt gttgcgattt tagaaggtgt       60

ccagtgtgag gtgcagctgg tggagtctgg gggaggcttg gtacagcccg gcaggtccct      120

gagactctcc tgtgcggcct ctggattcac ctttgatgat tatgccatgc actgggtccg      180

gcaagctcca gggaagggcc tggaatgggt ctcagctatc acttggaata gtggtcacat      240

agactatgcg gactctgtgg agggccgatt caccatctcc agagacaacg ccaagaactc      300

cctgtatctg caaatgaaca gtctgagagc tgaggatacg gccgtatatt actgtgcgaa      360

annnnnnnnn nnnnnnnnnn nnnnntccct tgactattgg ggccaaggta ccctggtcac      420

cgtctcgagc gctgcat                                                     437


<210>  10
<211>  395
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  libraries for variable light chain domains containing N-sequences
       in the LCDR3 and flanked by ClaI and Eco47III restriction sites


<220>
<221>  misc_feature
<222>  (337)..(351)
<223>  N = wildcard, nucleotide can be any of the four nucleotides 
       occuring in DNA

<400>  10
atggacatga gggtccctgc tcagctcctg ggactcctgc tgctctggct cccaggtgcc       60

agatgtgaca tccagatgac ccagtctcca tcctccctgt ctgcatctgt aggggacaga      120

gtcaccatca cttgtcgggc aagtcagggc atcagaaatt acttagcctg gtatcagcaa      180

aaaccaggga aagcccctaa gctcctgatc tatgctgcat ccactttgca atcaggggtc      240

ccatctcggt tcagtggcag tggatctggg acagatttca ctctcaccat cagcagccta      300

cagcctgaag atgttgcaac ttattactgt caaaggnnnn nnnnnnnnnn ntatactttt      360

ggccagggga ccaaggtgga aatcaagcgc tgcat                                 395


<210>  11
<211>  1784
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ORF of functional PiggyBac transposase enzyme

<400>  11
atgggtagtt ctttagacga tgagcatatc ctctctgctc ttctgcaaag cgatgacgag       60

cttgttggtg aggattctga cagtgaaata tcagatcacg taagtgaaga tgacgtccag      120

agcgatacag aagaagcgtt tatagatgag gtacatgaag tgcagccaac gtcaagcggt      180

agtgaaatat tagacgaaca aaatgttatt gaacaaccag gttcttcatt ggcttctaac      240

agaatcttga ccttgccaca gaggactatt agaggtaaga ataaacattg ttggtcaact      300

tcaaagtcca cgaggcgtag ccgagtctct gcactgaaca ttgtcagatc tcaaagaggt      360

ccgacgcgta tgtgccgcaa tatatatgac ccacttttat gcttcaaact attttttact      420

gatgagataa tttcggaaat tgtaaaatgg acaaatgctg agatatcatt gaaacgtcgg      480

gaatctatga caggtgctac atttcgtgac acgaatgaag atgaaatcta tgctttcttt      540

ggtattctgg taatgacagc agtgagaaaa gataaccaca tgtccacaga tgacctcttt      600

gatcgatctt tgtcaatggt gtacgtctct gtaatgagtc gtgatcgttt tgattttttg      660

atacgatgtc ttagaatgga tgacaaaagt atacggccca cacttcgaga aaacgatgta      720

tttactcctg ttagaaaaat atgggatctc tttatccatc agtgcataca aaattacact      780

ccaggggctc atttgaccat agatgaacag ttacttggtt ttagaggacg gtgtccgttt      840

aggatgtata tcccaaacaa gccaagtaag tatggaataa aaatcctcat gatgtgtgac      900

agtggtacga agtatatgat aaatggaatg ccttatttgg gaagaggaac acagaccaac      960

ggagtaccac tcggtgaata ctacgtgaag gagttatcaa agcctgtgca cggtagttgt     1020

cgtaatatta cgtgtgacaa ttggttcacc tcaatccctt tggcaaaaaa cttactacaa     1080

gaaccgtata agttaaccat tgtgggaacc gtgcgatcaa acaaacgcga gataccggaa     1140

gtactgaaaa acagtcgctc caggccagtg ggaacatcga tgttttgttt tgacggaccc     1200

cttactctcg tctcatataa accgaagcca gctaagatgg tatacttatt atcatcttgt     1260

gatgaggatg cttctatcaa cgaaagtacc ggtaaaccgc aaatggttat gtattataat     1320

caaactaaag gcggagtgga cacgctagac caaatgtgtt ctgtgatgac ctgcagtagg     1380

aagacgaata ggtggcctat ggcattattg tacggaatga taaacattgc ctgcataaat     1440

tcttttatta tatacagcca taatgtcagt agcaagggag aaaaggttca aagtcgcaaa     1500

aaatttatga gaaaccttta catgagcctg acgtcatcgt ttatgcgtaa gcgtttagaa     1560

gctcctactt tgaagagata tttgcgcgat aatatctcta atattttgcc aaatgaagtg     1620

cctggtacat cagatgacag tactgaagag ccagtaatga aaaaacgtac ttactgtact     1680

tactgcccct ctaaaataag gcgaaaggca aatgcatcgt gcaaaaaatg caaaaaagtt     1740

atttgtcgag agcataatat tgatatgtgc caaagttgtt ttag                      1784


<210>  12
<211>  593
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein sequence of functional PiggyBac transposase enzyme

<400>  12

Met Gly Ser Ser Leu Asp Asp Glu His Ile Leu Ser Ala Leu Leu Gln 
1               5                   10                  15      


Ser Asp Asp Glu Leu Val Gly Glu Asp Ser Asp Ser Glu Ile Ser Asp 
            20                  25                  30          


His Val Ser Glu Asp Asp Val Gln Ser Asp Thr Glu Glu Ala Phe Ile 
        35                  40                  45              


Asp Glu Val His Glu Val Gln Pro Thr Ser Ser Gly Ser Glu Ile Leu 
    50                  55                  60                  


Asp Glu Gln Asn Val Ile Glu Gln Pro Gly Ser Ser Leu Ala Ser Asn 
65                  70                  75                  80  


Arg Ile Leu Thr Leu Pro Gln Arg Thr Ile Arg Gly Lys Asn Lys His 
                85                  90                  95      


Cys Trp Ser Thr Ser Lys Ser Thr Arg Arg Ser Arg Val Ser Ala Leu 
            100                 105                 110         


Asn Ile Val Arg Ser Gln Arg Gly Pro Thr Arg Met Cys Arg Asn Ile 
        115                 120                 125             


Tyr Asp Pro Leu Leu Cys Phe Lys Leu Phe Phe Thr Asp Glu Ile Ile 
    130                 135                 140                 


Ser Glu Ile Val Lys Trp Thr Asn Ala Glu Ile Ser Leu Lys Arg Arg 
145                 150                 155                 160 


Glu Ser Met Thr Gly Ala Thr Phe Arg Asp Thr Asn Glu Asp Glu Ile 
                165                 170                 175     


Tyr Ala Phe Phe Gly Ile Leu Val Met Thr Ala Val Arg Lys Asp Asn 
            180                 185                 190         


His Met Ser Thr Asp Asp Leu Phe Asp Arg Ser Leu Ser Met Val Tyr 
        195                 200                 205             


Val Ser Val Met Ser Arg Asp Arg Phe Asp Phe Leu Ile Arg Cys Leu 
    210                 215                 220                 


Arg Met Asp Asp Lys Ser Ile Arg Pro Thr Leu Arg Glu Asn Asp Val 
225                 230                 235                 240 


Phe Thr Pro Val Arg Lys Ile Trp Asp Leu Phe Ile His Gln Cys Ile 
                245                 250                 255     


Gln Asn Tyr Thr Pro Gly Ala His Leu Thr Ile Asp Glu Gln Leu Leu 
            260                 265                 270         


Gly Phe Arg Gly Arg Cys Pro Phe Arg Met Tyr Ile Pro Asn Lys Pro 
        275                 280                 285             


Ser Lys Tyr Gly Ile Lys Ile Leu Met Met Cys Asp Ser Gly Thr Lys 
    290                 295                 300                 


Tyr Met Ile Asn Gly Met Pro Tyr Leu Gly Arg Gly Thr Gln Thr Asn 
305                 310                 315                 320 


Gly Val Pro Leu Gly Glu Tyr Tyr Val Lys Glu Leu Ser Lys Pro Val 
                325                 330                 335     


His Gly Ser Cys Arg Asn Ile Thr Cys Asp Asn Trp Phe Thr Ser Ile 
            340                 345                 350         


Pro Leu Ala Lys Asn Leu Leu Gln Glu Pro Tyr Lys Leu Thr Ile Val 
        355                 360                 365             


Gly Thr Val Arg Ser Asn Lys Arg Glu Ile Pro Glu Val Leu Lys Asn 
    370                 375                 380                 


Ser Arg Ser Arg Pro Val Gly Thr Ser Met Phe Cys Phe Asp Gly Pro 
385                 390                 395                 400 


Leu Thr Leu Val Ser Tyr Lys Pro Lys Pro Ala Lys Met Val Tyr Leu 
                405                 410                 415     


Leu Ser Ser Cys Asp Glu Asp Ala Ser Ile Asn Glu Ser Thr Gly Lys 
            420                 425                 430         


Pro Gln Met Val Met Tyr Tyr Asn Gln Thr Lys Gly Gly Val Asp Thr 
        435                 440                 445             


Leu Asp Gln Met Cys Ser Val Met Thr Cys Ser Arg Lys Thr Asn Arg 
    450                 455                 460                 


Trp Pro Met Ala Leu Leu Tyr Gly Met Ile Asn Ile Ala Cys Ile Asn 
465                 470                 475                 480 


Ser Phe Ile Ile Tyr Ser His Asn Val Ser Ser Lys Gly Glu Lys Val 
                485                 490                 495     


Gln Ser Arg Lys Lys Phe Met Arg Asn Leu Tyr Met Ser Leu Thr Ser 
            500                 505                 510         


Ser Phe Met Arg Lys Arg Leu Glu Ala Pro Thr Leu Lys Arg Tyr Leu 
        515                 520                 525             


Arg Asp Asn Ile Ser Asn Ile Leu Pro Asn Glu Val Pro Gly Thr Ser 
    530                 535                 540                 


Asp Asp Ser Thr Glu Glu Pro Val Met Lys Lys Arg Thr Tyr Cys Thr 
545                 550                 555                 560 


Tyr Cys Pro Ser Lys Ile Arg Arg Lys Ala Asn Ala Ser Cys Lys Lys 
                565                 570                 575     


Cys Lys Lys Val Ile Cys Arg Glu His Asn Ile Asp Met Cys Gln Ser 
            580                 585                 590         


Cys 
    


<210>  13
<211>  7385
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence of the PiggyBac expression construct 
       pCDNA3.1-hygro(+)-PB

<400>  13
gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg       60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg      120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc      180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt      240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata      300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc      360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc      420

attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt      480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt      540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca      600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg      660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc      720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg      780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca      840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc      900

gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc      960

agatatgggt agttctttag acgatgagca tatcctctct gctcttctgc aaagcgatga     1020

cgagcttgtt ggtgaggatt ctgacagtga aatatcagat cacgtaagtg aagatgacgt     1080

ccagagcgat acagaagaag cgtttataga tgaggtacat gaagtgcagc caacgtcaag     1140

cggtagtgaa atattagacg aacaaaatgt tattgaacaa ccaggttctt cattggcttc     1200

taacagaatc ttgaccttgc cacagaggac tattagaggt aagaataaac attgttggtc     1260

aacttcaaag tccacgaggc gtagccgagt ctctgcactg aacattgtca gatctcaaag     1320

aggtccgacg cgtatgtgcc gcaatatata tgacccactt ttatgcttca aactattttt     1380

tactgatgag ataatttcgg aaattgtaaa atggacaaat gctgagatat cattgaaacg     1440

tcgggaatct atgacaggtg ctacatttcg tgacacgaat gaagatgaaa tctatgcttt     1500

ctttggtatt ctggtaatga cagcagtgag aaaagataac cacatgtcca cagatgacct     1560

ctttgatcga tctttgtcaa tggtgtacgt ctctgtaatg agtcgtgatc gttttgattt     1620

tttgatacga tgtcttagaa tggatgacaa aagtatacgg cccacacttc gagaaaacga     1680

tgtatttact cctgttagaa aaatatggga tctctttatc catcagtgca tacaaaatta     1740

cactccaggg gctcatttga ccatagatga acagttactt ggttttagag gacggtgtcc     1800

gtttaggatg tatatcccaa acaagccaag taagtatgga ataaaaatcc tcatgatgtg     1860

tgacagtggt acgaagtata tgataaatgg aatgccttat ttgggaagag gaacacagac     1920

caacggagta ccactcggtg aatactacgt gaaggagtta tcaaagcctg tgcacggtag     1980

ttgtcgtaat attacgtgtg acaattggtt cacctcaatc cctttggcaa aaaacttact     2040

acaagaaccg tataagttaa ccattgtggg aaccgtgcga tcaaacaaac gcgagatacc     2100

ggaagtactg aaaaacagtc gctccaggcc agtgggaaca tcgatgtttt gttttgacgg     2160

accccttact ctcgtctcat ataaaccgaa gccagctaag atggtatact tattatcatc     2220

ttgtgatgag gatgcttcta tcaacgaaag taccggtaaa ccgcaaatgg ttatgtatta     2280

taatcaaact aaaggcggag tggacacgct agaccaaatg tgttctgtga tgacctgcag     2340

taggaagacg aataggtggc ctatggcatt attgtacgga atgataaaca ttgcctgcat     2400

aaattctttt attatataca gccataatgt cagtagcaag ggagaaaagg ttcaaagtcg     2460

caaaaaattt atgagaaacc tttacatgag cctgacgtca tcgtttatgc gtaagcgttt     2520

agaagctcct actttgaaga gatatttgcg cgataatatc tctaatattt tgccaaatga     2580

agtgcctggt acatcagatg acagtactga agagccagta atgaaaaaac gtacttactg     2640

tacttactgc ccctctaaaa taaggcgaaa ggcaaatgca tcgtgcaaaa aatgcaaaaa     2700

agttatttgt cgagagcata atattgatat gtgccaaagt tgttttagat ccagcacagt     2760

ggcggccgct cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt     2820

ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg     2880

ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt     2940

gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca     3000

atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct     3060

ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg     3120

tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt     3180

tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcggggca     3240

tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg     3300

gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg     3360

agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct     3420

cggtctattc ttttgattta taagggattt tggggatttc ggcctattgg ttaaaaaatg     3480

agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg     3540

tggaaagtcc ccaggctccc caggcaggca gaagtatgca aagcatgcat ctcaattagt     3600

cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc     3660

atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc     3720

cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg     3780

ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc     3840

taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg     3900

tgatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg     3960

acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg     4020

atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag     4080

atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca     4140

ttggggaatt cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt     4200

tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg     4260

atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag     4320

gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt     4380

atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg     4440

agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg     4500

gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg     4560

cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg     4620

cttgtatgga gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc     4680

cgcggctccg ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg     4740

acggcaattt cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg     4800

gagccgggac tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg     4860

gctgtgtaga agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa     4920

aggaatagca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct     4980

tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg     5040

agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata     5100

gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca     5160

aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt     5220

aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca     5280

tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat     5340

taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt     5400

aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct     5460

cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa     5520

aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa     5580

aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc     5640

tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga     5700

caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc     5760

cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt     5820

ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct     5880

gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg     5940

agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta     6000

gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct     6060

acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa     6120

gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt     6180

gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta     6240

cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat     6300

caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa     6360

gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct     6420

cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta     6480

cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct     6540

caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg     6600

gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa     6660

gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt     6720

cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta     6780

catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca     6840

gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta     6900

ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct     6960

gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg     7020

cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac     7080

tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact     7140

gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa     7200

atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt     7260

ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat     7320

gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg     7380

acgtc                                                                 7385


<210>  14
<211>  246
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  upstream Sleeping beauty 5'ITR sequence

<400>  14
atatcaattg agttgaagtc ggaagtttac atacacttaa gttggagtca ttaaaactcg       60

tttttcaact acaccacaaa tttcttgtta acaaacaata gttttggcaa gtcagttagg      120

acatctactt tgtgcatgac acaagtcatt tttccaacaa ttgtttacag acagattatt      180

tcacttataa ttcactgtat cacaattcca gtgggtcaga agtttacata cactaacaat      240

tgatat                                                                 246


<210>  15
<211>  248
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  downstream Sleeping beauty 3'ITR sequence

<400>  15
atatctcgag ttgagtgtat gttaacttct gacccactgg gaatgtgatg aaagaaataa       60

aagctgaaat gaatcattct ctctactatt attctgatat ttcacattct taaaataaag      120

tggtgatcct aactgacctt aagacaggga atctttactc ggattaaatg tcaggaattg      180

tgaaaaagtg agtttaaatg tatttggcta aggtgtatgt aaacttccga cttcaactct      240

cgagatat                                                               248


<210>  16
<211>  6339
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence of the human Ig-kappa LC expression vector 
       pIRES-EGFP-sbT1T2-IgL

<400>  16
gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg       60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg      120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgagtt gaagtcggaa      180

gtttacatac acttaagttg gagtcattaa aactcgtttt tcaactacac cacaaatttc      240

ttgttaacaa acaatagttt tggcaagtca gttaggacat ctactttgtg catgacacaa      300

gtcatttttc caacaattgt ttacagacag attatttcac ttataattca ctgtatcaca      360

attccagtgg gtcagaagtt tacatacact aacaattgca tgaagaatct gcttagggtt      420

aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg      480

actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc      540

cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca      600

ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt      660

caatgggtgg actatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg      720

ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag      780

tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt      840

accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg      900

ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa      960

cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt     1020

gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc cactgcttac     1080

tggcttatcg aaattaatac gactcactat agggagaccc aagcttggta ccgagctcgg     1140

atcgatatgg acatgagggt ccctgctcag ctcctgggac tcctgctgct ctggctccca     1200

ggtgccagat gtgacatcca gatgacccag tctccatcct ccctgtctgc atctgtaggg     1260

gacagagtca ccatcacttg tcgggcaagt cagggcatca gaaattactt agcctggtat     1320

cagcaaaaac cagggaaagc ccctaagctc ctgatctatg ctgcatccac tttgcaatca     1380

ggggtcccat ctcggttcag tggcagtgga tctgggacag atttcactct caccatcagc     1440

agcctacagc ctgaagatgt tgcaacttat tactgtcaaa ggtataaccg tgcaccgtat     1500

acttttggcc aggggaccaa ggtggaaatc aagcgctctg tggctgcacc atctgtcttc     1560

atcttcccgc catctgatga gcagttgaaa tctggaactg cctctgttgt gtgcctgctg     1620

aataacttct atcccagaga ggccaaagta cagtggaagg tggataacgc cctccaatcg     1680

ggtaactccc aggagagtgt cacagagcag gacagcaagg acagcaccta cagcctcagc     1740

agcaccctga cgctgagcaa agcagactac gagaaacaca aagtctacgc ctgcgaagtc     1800

acccatcagg gcctgagctc gcccgtcaca aagagcttca acaggggaga gtgttaaatc     1860

tgcggccgcg tcgacggaat tcagtggatc cactagtaac ggccgccagt gtgctggaat     1920

taattcgctg tctgcgaggg ccagctgttg gggtgagtac tccctctcaa aagcgggcat     1980

gacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc     2040

cgcggtgatg cctttgaggg tggccgcgtc catctggtca gaaaagacaa tctttttgtt     2100

gtcaagcttg aggtgtggca ggcttgagat ctggccatac acttgagtga caatgacatc     2160

cactttgcct ttctctccac aggtgtccac tcccaggtcc aactgcaggt cgagcatgca     2220

tctagggcgg ccaattccgc ccctctccct cccccccccc taacgttact ggccgaagcc     2280

gcttggaata aggccggtgt gcgtttgtct atatgtgatt ttccaccata ttgccgtctt     2340

ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc     2400

tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc     2460

tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc     2520

cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg     2580

cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct     2640

cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat     2700

ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc     2760

taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga taagcttgcc     2820

acaacccggg atccaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg     2880

gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc     2940

ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc     3000

ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc     3060

ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa     3120

ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc     3180

gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc     3240

aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc     3300

tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac     3360

atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac     3420

ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac     3480

cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact     3540

ctcggcatgg acgagctgta caagtaaagc ggccctagag ctcgctgatc agcctcgact     3600

gtgcctctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg     3660

aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga     3720

gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg     3780

aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa     3840

ccagctgggg ctcgagttga gtgtatgtta acttctgacc cactgggaat gtgatgaaag     3900

aaataaaagc tgaaatgaat cattctctct actattattc tgatatttca cattcttaaa     3960

ataaagtggt gatcctaact gaccttaaga cagggaatct ttactcggat taaatgtcag     4020

gaattgtgaa aaagtgagtt taaatgtatt tggctaaggt gtatgtaaac ttccgacttc     4080

aactctcgag tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc     4140

tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg     4200

tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa     4260

gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct     4320

ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga     4380

ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc     4440

gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa     4500

tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt     4560

aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa     4620

aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt     4680

ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg     4740

tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc     4800

agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc     4860

gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta     4920

tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct     4980

acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc     5040

tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa     5100

caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa     5160

aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa     5220

aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt     5280

ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac     5340

agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc     5400

atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc     5460

cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata     5520

aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc     5580

cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc     5640

aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca     5700

ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa     5760

gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca     5820

ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt     5880

tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt     5940

tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg     6000

ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga     6060

tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc     6120

agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg     6180

acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag     6240

ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg     6300

gttccgcgca catttccccg aaaagtgcca cctgacgtc                            6339


<210>  17
<211>  1023
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  open reading frame (ORF) of the Sleeping Beauty transposase 
       enzyme

<400>  17
atgggaaaat caaaagaaat cagccaagac ctcagaaaaa aaattgtaga cctccacaag       60

tctggttcat ccttgggagc aatttccaaa cgcctgaaag taccacgttc atctgtacaa      120

acaatagtac gcaagtataa acaccatggg accacgcagc cgtcataccg ctcaggaagg      180

agacgcgttc tgtctcctag agatgaacgt actttggtgc gaaaagtgca aatcaatccc      240

agaacaacag caaaggacct tgtgaagatg ctggaggaaa caggtacaaa agtatctata      300

tccacagtaa aacgagtcct atatcgacat aacctgaaag gccgctcagc aaggaagaag      360

ccactgctcc aaaaccgaca taagaaagcc agactacggt ttgcaactgc acatggggac      420

aaagatcgta ctttttggag aaatgtcctc tggtctgatg aaacaaaaat agaactgttt      480

ggccataatg accatcgtta tgtttggagg aagaaggggg aggcttgcaa gccgaagaac      540

accatcccaa ccgtgaagca cgggggtggc agcatcatgt tgtgggggtg ctttgctgca      600

ggagggactg gtgcacttca caaaatagat ggcatcatga ggaaggaaaa ttatgtggat      660

atattgaagc aacatctcaa gacatcagtc aggaagttaa agcttggtcg caaatgggtc      720

ttccaaatgg acaatgaccc caagcatact tccaaagttg tggcaaaatg gcttaaggac      780

aacaaagtca aggtattgga gtggccatca caaagccctg acctcaatcc tatagaaaat      840

ttgtgggcag aactgaaaaa gcgtgtgcga gcaaggaggc ctacaaacct gactcagtta      900

caccagctct gtcaggagga atgggccaaa attcacccaa cttattgtgg gaagcttgtg      960

gaaggctacc cgaaacgttt gacccaagtt aaacaattta aaggcaatgc taccaaatac     1020

tag                                                                   1023


<210>  18
<211>  340
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein sequence of the Sleeping Beauty transposase enzyme

<400>  18

Met Gly Lys Ser Lys Glu Ile Ser Gln Asp Leu Arg Lys Lys Ile Val 
1               5                   10                  15      


Asp Leu His Lys Ser Gly Ser Ser Leu Gly Ala Ile Ser Lys Arg Leu 
            20                  25                  30          


Lys Val Pro Arg Ser Ser Val Gln Thr Ile Val Arg Lys Tyr Lys His 
        35                  40                  45              


His Gly Thr Thr Gln Pro Ser Tyr Arg Ser Gly Arg Arg Arg Val Leu 
    50                  55                  60                  


Ser Pro Arg Asp Glu Arg Thr Leu Val Arg Lys Val Gln Ile Asn Pro 
65                  70                  75                  80  


Arg Thr Thr Ala Lys Asp Leu Val Lys Met Leu Glu Glu Thr Gly Thr 
                85                  90                  95      


Lys Val Ser Ile Ser Thr Val Lys Arg Val Leu Tyr Arg His Asn Leu 
            100                 105                 110         


Lys Gly Arg Ser Ala Arg Lys Lys Pro Leu Leu Gln Asn Arg His Lys 
        115                 120                 125             


Lys Ala Arg Leu Arg Phe Ala Thr Ala His Gly Asp Lys Asp Arg Thr 
    130                 135                 140                 


Phe Trp Arg Asn Val Leu Trp Ser Asp Glu Thr Lys Ile Glu Leu Phe 
145                 150                 155                 160 


Gly His Asn Asp His Arg Tyr Val Trp Arg Lys Lys Gly Glu Ala Cys 
                165                 170                 175     


Lys Pro Lys Asn Thr Ile Pro Thr Val Lys His Gly Gly Gly Ser Ile 
            180                 185                 190         


Met Leu Trp Gly Cys Phe Ala Ala Gly Gly Thr Gly Ala Leu His Lys 
        195                 200                 205             


Ile Asp Gly Ile Met Arg Lys Glu Asn Tyr Val Asp Ile Leu Lys Gln 
    210                 215                 220                 


His Leu Lys Thr Ser Val Arg Lys Leu Lys Leu Gly Arg Lys Trp Val 
225                 230                 235                 240 


Phe Gln Met Asp Asn Asp Pro Lys His Thr Ser Lys Val Val Ala Lys 
                245                 250                 255     


Trp Leu Lys Asp Asn Lys Val Lys Val Leu Glu Trp Pro Ser Gln Ser 
            260                 265                 270         


Pro Asp Leu Asn Pro Ile Glu Asn Leu Trp Ala Glu Leu Lys Lys Arg 
        275                 280                 285             


Val Arg Ala Arg Arg Pro Thr Asn Leu Thr Gln Leu His Gln Leu Cys 
    290                 295                 300                 


Gln Glu Glu Trp Ala Lys Ile His Pro Thr Tyr Cys Gly Lys Leu Val 
305                 310                 315                 320 


Glu Gly Tyr Pro Lys Arg Leu Thr Gln Val Lys Gln Phe Lys Gly Asn 
                325                 330                 335     


Ala Thr Lys Tyr 
            340 


<210>  19
<211>  6624
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence of the Sleeping Beauty expression construct 
       pCDNA3.1-hygro(+)-SB

<400>  19
gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg       60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg      120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc      180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt      240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata      300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc      360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc      420

attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt      480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt      540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca      600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg      660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc      720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg      780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca      840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc      900

gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc      960

agatatggga aaatcaaaag aaatcagcca agacctcaga aaaaaaattg tagacctcca     1020

caagtctggt tcatccttgg gagcaatttc caaacgcctg aaagtaccac gttcatctgt     1080

acaaacaata gtacgcaagt ataaacacca tgggaccacg cagccgtcat accgctcagg     1140

aaggagacgc gttctgtctc ctagagatga acgtactttg gtgcgaaaag tgcaaatcaa     1200

tcccagaaca acagcaaagg accttgtgaa gatgctggag gaaacaggta caaaagtatc     1260

tatatccaca gtaaaacgag tcctatatcg acataacctg aaaggccgct cagcaaggaa     1320

gaagccactg ctccaaaacc gacataagaa agccagacta cggtttgcaa ctgcacatgg     1380

ggacaaagat cgtacttttt ggagaaatgt cctctggtct gatgaaacaa aaatagaact     1440

gtttggccat aatgaccatc gttatgtttg gaggaagaag ggggaggctt gcaagccgaa     1500

gaacaccatc ccaaccgtga agcacggggg tggcagcatc atgttgtggg ggtgctttgc     1560

tgcaggaggg actggtgcac ttcacaaaat agatggcatc atgaggaagg aaaattatgt     1620

ggatatattg aagcaacatc tcaagacatc agtcaggaag ttaaagcttg gtcgcaaatg     1680

ggtcttccaa atggacaatg accccaagca tacttccaaa gttgtggcaa aatggcttaa     1740

ggacaacaaa gtcaaggtat tggagtggcc atcacaaagc cctgacctca atcctataga     1800

aaatttgtgg gcagaactga aaaagcgtgt gcgagcaagg aggcctacaa acctgactca     1860

gttacaccag ctctgtcagg aggaatgggc caaaattcac ccaacttatt gtgggaagct     1920

tgtggaaggc tacccgaaac gtttgaccca agttaaacaa tttaaaggca atgctaccaa     1980

atactagatc cagcacagtg gcggccgctc gagtctagag ggcccgttta aacccgctga     2040

tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct     2100

tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca     2160

tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag     2220

ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct     2280

gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca     2340

ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta     2400

gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt     2460

caagctctaa atcggggcat ccctttaggg ttccgattta gtgctttacg gcacctcgac     2520

cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt     2580

tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga     2640

acaacactca accctatctc ggtctattct tttgatttat aagggatttt ggggatttcg     2700

gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga     2760

atgtgtgtca gttagggtgt ggaaagtccc caggctcccc aggcaggcag aagtatgcaa     2820

agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc     2880

agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg     2940

cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt     3000

ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga     3060

ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt     3120

ttcggatctg atcagcacgt gatgaaaaag cctgaactca ccgcgacgtc tgtcgagaag     3180

tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa     3240

tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc     3300

gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg     3360

attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc     3420

cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag     3480

ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc     3540

ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg     3600

attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc     3660

gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac     3720

ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg     3780

gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc     3840

ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat     3900

ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa     3960

ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc     4020

gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc     4080

gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc     4140

agcactcgtc cgagggcaaa ggaatagcac gtgctacgag atttcgattc caccgccgcc     4200

ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag     4260

cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat     4320

ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat     4380

tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc     4440

tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg     4500

ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa     4560

tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac     4620

ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt     4680

gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga     4740

gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca     4800

ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg     4860

ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt     4920

cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc     4980

ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct     5040

tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc     5100

gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta     5160

tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca     5220

gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag     5280

tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag     5340

ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt     5400

agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa     5460

gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg     5520

attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga     5580

agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta     5640

atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc     5700

cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg     5760

ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga     5820

agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt     5880

tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt     5940

gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc     6000

caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc     6060

ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca     6120

gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag     6180

tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg     6240

tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa     6300

cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa     6360

cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga     6420

gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga     6480

atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg     6540

agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt     6600

ccccgaaaag tgccacctga cgtc                                            6624


<210>  20
<211>  4975
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence containing a PiggyBac ITR-flanked expression 
       cassette for membrane spanning human Ig-gamma1 heavy chains

<400>  20
gaattcttaa ccctagaaag atagtctgcg taaaattgac gcatgcattc ttgaaatatt       60

gctctctctt tctaaatagc gcgaatccgt cgctgtgcat ttaggacatc tcagtcgccg      120

cttggagctc ccgtgaggcg tgcttgtcaa tgcggtaagt gtcactgatt ttgaactata      180

acgaccgcgt gagtcaaaat gacgcatgat tatcttttac gtgactttta agatttaact      240

catacgataa ttatattgtt atttcatgtt ctacttacgt gataacttat tatatatata      300

ttttcttgtt atacctcgag gctagtctag agagtaattc atacaaaagg actcgcccct      360

gccttgggga atcccaggga ccgtcgttaa actcccacta acgtagaacc cagagatcgc      420

tgcgttcccg ccccctcacc cgcccgctct cgtcatcact gaggtggaga agagcatgcg      480

tgaggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg      540

gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa      600

agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt      660

gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt      720

gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga      780

attacttcca cgcccctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag      840

tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga      900

ggcctggctt gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct      960

cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt     1020

tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt     1080

tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg     1140

cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct     1200

ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc     1260

ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa     1320

atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc     1380

ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca     1440

cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta     1500

tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt     1560

gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc     1620

tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgagcgg ccgctagatt     1680

gctagcacca agggcccatc ggtcttcccc ctggcaccct cctccaagag cacctctggg     1740

ggcacagcag ccctgggctg cctggtcaag gactacttcc ccgaaccggt gacggtgtcg     1800

tggaactcag gcgccctgac cagcggcgtg cacaccttcc cggctgtcct acagtcctca     1860

ggactctact ccctcagcag cgtggtgacc gtgccctcca gcagcttggg cacccagacc     1920

tacatctgca acgtgaatca caagcccagc aacaccaagg tggacaagaa agttgagccc     1980

aaatcttgtg acaaaactca cacatgccca ccgtgcccag cacctgaact cctgggggga     2040

ccgtcagtct tcctcttccc cccaaaaccc aaggacaccc tcatgatctc ccggacccct     2100

gaggtcacat gcgtggtggt ggacgtgagc cacgaagacc ctgaggtcaa gttcaactgg     2160

tacgtggacg gcgtggaggt gcataatgcc aagacaaagc cgcgggagga gcagtacaac     2220

agcacgtacc gtgtggtcag cgtcctcacc gtcctgcacc aggactggct gaatggcaag     2280

gagtacaagt gcaaggtctc caacaaagcc ctcccagccc ccatcgagaa aaccatctcc     2340

aaagccaaag ggcagccccg agaaccacag gtgtacaccc tgcccccatc ccgggatgag     2400

ctgaccaaga accaggtcag cctgacctgc ctggtcaaag gcttctatcc cagcgacatc     2460

gccgtggagt gggagagcaa tgggcagccg gagaacaact acaagaccac gcctcccgtg     2520

ctggactccg acggctcctt cttcctctac agcaagctca ccgtggacaa gagcaggtgg     2580

cagcagggga acgtcttctc atgctccgtg atgcatgagg ctctgcacaa ccactacaca     2640

cagaagagcc tctccctgtc tccggagctg caactggagg agagctgtgc ggaggcgcag     2700

gacggggagc tggacgggct gtggacgacc atcaccatct tcatcacact cttcctgtta     2760

agcgtgtgct acagtgccac cgtcaccttc ttcaaggtga agtggatctt ctcctcggtg     2820

gtggacctga agcagaccat catccccgac tacaggaaca tgatcggaca gggggcctag     2880

taagcttagc gctttcgaag ggcaggtaag tatcaaggtt acaagacagg tttaaggaga     2940

ccaatagaaa ctgggcttgt cgagacagag aagactcttg cgtttctgat aggcacctat     3000

tggtcttact gacatccact ttgcctttct ctccacaggt gtcctacgta gcgatcgcca     3060

attccgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg     3120

ccggtgtgcg tttgtctata tgtgattttc caccatattg ccgtcttttg gcaatgtgag     3180

ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc     3240

caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg     3300

aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag     3360

gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca     3420

gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt     3480

caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc     3540

tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa     3600

ccacggggac gtggttttcc tttgaaaaac acgatgataa gcttgccaca acccggttta     3660

aacggatccc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat     3720

cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga     3780

gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc     3840

cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta     3900

ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca     3960

ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt     4020

cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg     4080

caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc     4140

cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg     4200

cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct     4260

gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa     4320

gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga     4380

cgagctgtac aagtaaagcg gcatatgcgc cggcggatat ccggcgcgcc gagctcgctg     4440

atcagcctcg actgtgcctc tagttgccag ccatctgttg tttgcccctc ccccgtgcct     4500

tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca     4560

tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag     4620

ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct     4680

gaggcggaaa gaaccagctg gggttaatta aacgcgttta accctagaaa gataatcata     4740

ttgtgacgta cgttaaagat aatcatgcgt aaaattgacg catgtgtttt atcggtctgt     4800

atatcgaggt ttatttatta atttgaatag atattaagtt ttattatatt tacacttaca     4860

tactaataat aaattcaaca aacaatttat ttatgtttat ttatttatta aaaaaaaaca     4920

aaaactcaaa atttcttcta taaagtaaca aaacttttat atcgattcgc gcgcg          4975


<210>  21
<211>  2774
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence containing vector backbone components ColE1 and 
       ampicillin resistance flanked by 5' and 3' ITRs of Sleeping 
       Beauty

<400>  21
ttaattaaac gcgtttgagt gtatgttaac ttctgaccca ctgggaatgt gatgaaagaa       60

ataaaagctg aaatgaatca ttctctctac tattattctg atatttcaca ttcttaaaat      120

aaagtggtga tcctaactga ccttaagaca gggaatcttt actcggatta aatgtcagga      180

attgtgaaaa agtgagttta aatgtatttg gctaaggtgt atgtaaactt ccgacttcaa      240

ctatcgattc gcgcgcgtgc attctagttg tggtttgtcc aaactcatca atgtatctta      300

tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt      360

tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa      420

gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact      480

gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc      540

ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg      600

ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc      660

cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag      720

gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca      780

tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca      840

ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg      900

atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag      960

gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt     1020

tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca     1080

cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg     1140

cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt     1200

tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc     1260

cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg     1320

cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg     1380

gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta     1440

gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg     1500

gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg     1560

ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc     1620

atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc     1680

agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc     1740

ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag     1800

tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat     1860

ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg     1920

caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt     1980

gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag     2040

atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg     2100

accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt     2160

aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct     2220

gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac     2280

tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat     2340

aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat     2400

ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca     2460

aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggcct     2520

gcaggaattc agttgaagtc ggaagtttac atacacttaa gttggagtca ttaaaactcg     2580

tttttcaact acaccacaaa tttcttgtta acaaacaata gttttggcaa gtcagttagg     2640

acatctactt tgtgcatgac acaagtcatt tttccaacaa ttgtttacag acagattatt     2700

tcacttataa ttcactgtat cacaattcca gtgggtcaga agtttacata cactaacctc     2760

gaggctagtc taga                                                       2774


<210>  22
<211>  7242
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence of PiggyBac transposable "empty" human gamma1-HC vector

<400>  22
gaattcttaa ccctagaaag atagtctgcg taaaattgac gcatgcattc ttgaaatatt       60

gctctctctt tctaaatagc gcgaatccgt cgctgtgcat ttaggacatc tcagtcgccg      120

cttggagctc ccgtgaggcg tgcttgtcaa tgcggtaagt gtcactgatt ttgaactata      180

acgaccgcgt gagtcaaaat gacgcatgat tatcttttac gtgactttta agatttaact      240

catacgataa ttatattgtt atttcatgtt ctacttacgt gataacttat tatatatata      300

ttttcttgtt atacctcgag gctagtctag agagtaattc atacaaaagg actcgcccct      360

gccttgggga atcccaggga ccgtcgttaa actcccacta acgtagaacc cagagatcgc      420

tgcgttcccg ccccctcacc cgcccgctct cgtcatcact gaggtggaga agagcatgcg      480

tgaggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg      540

gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa      600

agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt      660

gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt      720

gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga      780

attacttcca cgcccctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag      840

tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga      900

ggcctggctt gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct      960

cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt     1020

tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt     1080

tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg     1140

cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct     1200

ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc     1260

ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa     1320

atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc     1380

ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca     1440

cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta     1500

tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt     1560

gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc     1620

tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgagcgg ccgctagatt     1680

gctagcacca agggcccatc ggtcttcccc ctggcaccct cctccaagag cacctctggg     1740

ggcacagcag ccctgggctg cctggtcaag gactacttcc ccgaaccggt gacggtgtcg     1800

tggaactcag gcgccctgac cagcggcgtg cacaccttcc cggctgtcct acagtcctca     1860

ggactctact ccctcagcag cgtggtgacc gtgccctcca gcagcttggg cacccagacc     1920

tacatctgca acgtgaatca caagcccagc aacaccaagg tggacaagaa agttgagccc     1980

aaatcttgtg acaaaactca cacatgccca ccgtgcccag cacctgaact cctgggggga     2040

ccgtcagtct tcctcttccc cccaaaaccc aaggacaccc tcatgatctc ccggacccct     2100

gaggtcacat gcgtggtggt ggacgtgagc cacgaagacc ctgaggtcaa gttcaactgg     2160

tacgtggacg gcgtggaggt gcataatgcc aagacaaagc cgcgggagga gcagtacaac     2220

agcacgtacc gtgtggtcag cgtcctcacc gtcctgcacc aggactggct gaatggcaag     2280

gagtacaagt gcaaggtctc caacaaagcc ctcccagccc ccatcgagaa aaccatctcc     2340

aaagccaaag ggcagccccg agaaccacag gtgtacaccc tgcccccatc ccgggatgag     2400

ctgaccaaga accaggtcag cctgacctgc ctggtcaaag gcttctatcc cagcgacatc     2460

gccgtggagt gggagagcaa tgggcagccg gagaacaact acaagaccac gcctcccgtg     2520

ctggactccg acggctcctt cttcctctac agcaagctca ccgtggacaa gagcaggtgg     2580

cagcagggga acgtcttctc atgctccgtg atgcatgagg ctctgcacaa ccactacaca     2640

cagaagagcc tctccctgtc tccggagctg caactggagg agagctgtgc ggaggcgcag     2700

gacggggagc tggacgggct gtggacgacc atcaccatct tcatcacact cttcctgtta     2760

agcgtgtgct acagtgccac cgtcaccttc ttcaaggtga agtggatctt ctcctcggtg     2820

gtggacctga agcagaccat catccccgac tacaggaaca tgatcggaca gggggcctag     2880

taagcttagc gctttcgaag ggcaggtaag tatcaaggtt acaagacagg tttaaggaga     2940

ccaatagaaa ctgggcttgt cgagacagag aagactcttg cgtttctgat aggcacctat     3000

tggtcttact gacatccact ttgcctttct ctccacaggt gtcctacgta gcgatcgcca     3060

attccgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg     3120

ccggtgtgcg tttgtctata tgtgattttc caccatattg ccgtcttttg gcaatgtgag     3180

ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc     3240

caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg     3300

aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag     3360

gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca     3420

gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt     3480

caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc     3540

tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa     3600

ccacggggac gtggttttcc tttgaaaaac acgatgataa gcttgccaca acccggttta     3660

aacggatccc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat     3720

cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga     3780

gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc     3840

cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta     3900

ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca     3960

ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt     4020

cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg     4080

caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc     4140

cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg     4200

cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct     4260

gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa     4320

gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga     4380

cgagctgtac aagtaaagcg gcatatgcgc cggcggatat ccggcgcgcc gagctcgctg     4440

atcagcctcg actgtgcctc tagttgccag ccatctgttg tttgcccctc ccccgtgcct     4500

tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca     4560

tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag     4620

ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct     4680

gaggcggaaa gaaccagctg gggttaatta aacgcgttta accctagaaa gataatcata     4740

ttgtgacgta cgttaaagat aatcatgcgt aaaattgacg catgtgtttt atcggtctgt     4800

atatcgaggt ttatttatta atttgaatag atattaagtt ttattatatt tacacttaca     4860

tactaataat aaattcaaca aacaatttat ttatgtttat ttatttatta aaaaaaaaca     4920

aaaactcaaa atttcttcta taaagtaaca aaacttttat atcgattcgc gcgcgtgcat     4980

tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc     5040

tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg     5100

ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa     5160

tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac     5220

ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt     5280

gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga     5340

gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca     5400

ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg     5460

ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt     5520

cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc     5580

ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct     5640

tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc     5700

gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta     5760

tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca     5820

gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag     5880

tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag     5940

ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt     6000

agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa     6060

gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg     6120

attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga     6180

agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta     6240

atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc     6300

cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg     6360

ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga     6420

agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt     6480

tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt     6540

gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc     6600

caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc     6660

ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca     6720

gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag     6780

tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg     6840

tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa     6900

cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa     6960

cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga     7020

gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga     7080

atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg     7140

agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt     7200

ccccgaaaag tgccacctga cgtcgacgga tcgggcctgc ag                        7242


<210>  23
<211>  7146
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence of Sleeping Beauty transposable "empty" human gamma1-HC 
       vector

<400>  23
gaattcagtt gaagtcggaa gtttacatac acttaagttg gagtcattaa aactcgtttt       60

tcaactacac cacaaatttc ttgttaacaa acaatagttt tggcaagtca gttaggacat      120

ctactttgtg catgacacaa gtcatttttc caacaattgt ttacagacag attatttcac      180

ttataattca ctgtatcaca attccagtgg gtcagaagtt tacatacact aacctcgagg      240

ctagtctaga gagtaattca tacaaaagga ctcgcccctg ccttggggaa tcccagggac      300

cgtcgttaaa ctcccactaa cgtagaaccc agagatcgct gcgttcccgc cccctcaccc      360

gcccgctctc gtcatcactg aggtggagaa gagcatgcgt gaggctccgg tgcccgtcag      420

tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga      480

accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc      540

cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt      600

ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg      660

cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac gcccctggct      720

gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc      780

ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcttg ggcgctgggg      840

ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct      900

agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt      960

aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg     1020

ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag     1080

aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc     1140

gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga     1200

aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg     1260

agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc     1320

ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt     1380

ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac     1440

tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt     1500

tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt     1560

ttttcttcca tttcaggtgt cgtgagcggc cgctagattg ctagcaccaa gggcccatcg     1620

gtcttccccc tggcaccctc ctccaagagc acctctgggg gcacagcagc cctgggctgc     1680

ctggtcaagg actacttccc cgaaccggtg acggtgtcgt ggaactcagg cgccctgacc     1740

agcggcgtgc acaccttccc ggctgtccta cagtcctcag gactctactc cctcagcagc     1800

gtggtgaccg tgccctccag cagcttgggc acccagacct acatctgcaa cgtgaatcac     1860

aagcccagca acaccaaggt ggacaagaaa gttgagccca aatcttgtga caaaactcac     1920

acatgcccac cgtgcccagc acctgaactc ctggggggac cgtcagtctt cctcttcccc     1980

ccaaaaccca aggacaccct catgatctcc cggacccctg aggtcacatg cgtggtggtg     2040

gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg     2100

cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc     2160

gtcctcaccg tcctgcacca ggactggctg aatggcaagg agtacaagtg caaggtctcc     2220

aacaaagccc tcccagcccc catcgagaaa accatctcca aagccaaagg gcagccccga     2280

gaaccacagg tgtacaccct gcccccatcc cgggatgagc tgaccaagaa ccaggtcagc     2340

ctgacctgcc tggtcaaagg cttctatccc agcgacatcg ccgtggagtg ggagagcaat     2400

gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga cggctccttc     2460

ttcctctaca gcaagctcac cgtggacaag agcaggtggc agcaggggaa cgtcttctca     2520

tgctccgtga tgcatgaggc tctgcacaac cactacacac agaagagcct ctccctgtct     2580

ccggagctgc aactggagga gagctgtgcg gaggcgcagg acggggagct ggacgggctg     2640

tggacgacca tcaccatctt catcacactc ttcctgttaa gcgtgtgcta cagtgccacc     2700

gtcaccttct tcaaggtgaa gtggatcttc tcctcggtgg tggacctgaa gcagaccatc     2760

atccccgact acaggaacat gatcggacag ggggcctagt aagcttagcg ctttcgaagg     2820

gcaggtaagt atcaaggtta caagacaggt ttaaggagac caatagaaac tgggcttgtc     2880

gagacagaga agactcttgc gtttctgata ggcacctatt ggtcttactg acatccactt     2940

tgcctttctc tccacaggtg tcctacgtag cgatcgccaa ttccgcccct ctccctcccc     3000

cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat     3060

gtgattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt     3120

cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt     3180

gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc     3240

gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc     3300

acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat     3360

agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc     3420

ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg     3480

tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct     3540

ttgaaaaaca cgatgataag cttgccacaa cccggtttaa acggatcccg ccaccatggt     3600

gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga     3660

cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa     3720

gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt     3780

gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca     3840

cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa     3900

ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa     3960

ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct     4020

ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat     4080

caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca     4140

ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct     4200

gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct     4260

ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaaagcgg     4320

catatgcgcc ggcggatatc cggcgcgccg agctcgctga tcagcctcga ctgtgcctct     4380

agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc     4440

actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt     4500

cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat     4560

agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg     4620

ggttaattaa acgcgtttga gtgtatgtta acttctgacc cactgggaat gtgatgaaag     4680

aaataaaagc tgaaatgaat cattctctct actattattc tgatatttca cattcttaaa     4740

ataaagtggt gatcctaact gaccttaaga cagggaatct ttactcggat taaatgtcag     4800

gaattgtgaa aaagtgagtt taaatgtatt tggctaaggt gtatgtaaac ttccgacttc     4860

aactatcgat tcgcgcgcgt gcattctagt tgtggtttgt ccaaactcat caatgtatct     4920

tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg     4980

tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata     5040

aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca     5100

ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc     5160

gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg     5220

cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta     5280

tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc     5340

aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag     5400

catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac     5460

caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc     5520

ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt     5580

aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc     5640

gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga     5700

cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta     5760

ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta     5820

tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga     5880

tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg     5940

cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag     6000

tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc     6060

tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact     6120

tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt     6180

cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta     6240

ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta     6300

tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc     6360

gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat     6420

agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt     6480

atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg     6540

tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca     6600

gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta     6660

agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg     6720

cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact     6780

ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg     6840

ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt     6900

actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga     6960

ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc     7020

atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa     7080

caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcgggc     7140

ctgcag                                                                7146


<210>  24
<211>  351
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VH coding region of anti-human CD30 antibody brentuximab

<400>  24
cagatccagc tgcagcagtc tggacctgag gtggtgaagc ctggggcttc agtgaagata       60

tcctgcaagg cttctggcta caccttcact gactactata taacctgggt gaagcagaag      120

cctggacagg gacttgagtg gattggatgg atttatcctg gaagcggtaa tactaagtac      180

aatgagaagt tcaagggcaa ggccacattg actgtagaca catcctccag cacagccttc      240

atgcagctca gcagcctgac atctgaggac actgctgtct atttctgtgc gaactatggt      300

aactactggt ttgcttactg gggccaaggg actcaggtca ctgtctctgc a               351


<210>  25
<211>  333
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VL coding region of anti-human CD30 antibody brentuximab

<400>  25
gacattgtgc tgacccaatc tccagcttct ttggctgtgt ctctagggca gagggccacc       60

atctcctgca aggccagcca aagtgttgat tttgatggtg atagttatat gaactggtac      120

caacagaaac caggacagcc acccaaagtc ctcatctatg ctgcatccaa tctagaatct      180

gggatcccag ccaggtttag tggcagtggg tctgggacag acttcaccct caacatccat      240

cctgtggagg aggaggatgc tgcaacctat tactgtcagc aaagtaatga ggatccgtgg      300

acgttcggtg gaggcaccaa gctggaaatc aaa                                   333


<210>  26
<211>  417
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VH coding region of anti-human CD19 mAb huB12, including leader 
       sequence

<400>  26
atgggatgga gctggatctt tcttttcctc ctgtcaggaa ctgcaggtgt ccattgtcag       60

gttcagctgc aagagtctgg ccctgggttg gttaagccct cccagaccct cagtctgact      120

tgtactgtgt ctgggggttc aatcagcact tctggtatgg gtgtaggctg gattaggcag      180

cacccaggga agggtctgga gtggattgga cacatttggt gggatgatga caagagatat      240

aacccagccc tgaagagcag agtgacaatc tctgtggata cctccaagaa ccagtttagc      300

ctcaagctgt ccagtgtgac agctgcagat actgctgtct actactgtgc tagaatggaa      360

ctttggtcct actattttga ctactggggc caaggcaccc ttgtcacagt ctcctca         417


<210>  27
<211>  375
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VL coding region of anti-human CD19 mAb huB12, including leader 
       sequence

<400>  27
atgaagttgc ctgttaggct gttggtgctg atgttctgga ttcctgcttc cagcagtgaa       60

attgttctca cccagtctcc agcaaccctg tctctctctc caggggaaag ggctaccctg      120

agctgcagtg ccagctcaag tgtaagttac atgcactggt accagcagaa gccagggcag      180

gctcccagac tcctgattta tgacacatcc aaactggctt ctggtattcc agcaaggttc      240

agtggcagtg ggtctggaac agattttaca ctcacaatca gcagcctgga gccagaggat      300

gttgctgtct attactgttt tcaggggagt gtatacccat tcacttttgg ccaagggaca      360

aagttggaaa tcaaa                                                       375


<210>  28
<211>  423
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA fragment, containing NotI-NheI-flanked VH coding region of 
       the VH domain of anti-human CD30 mAb brentuximab

<400>  28
gcggccgcca tgaattttgg actgaggctg attttcctgg tgctgaccct gaaaggcgtc       60

cagtgtcaga tccagctgca gcagtctgga cctgaggtgg tgaagcctgg ggcttcagtg      120

aagatatcct gcaaggcttc tggctacacc ttcactgact actatataac ctgggtgaag      180

cagaagcctg gacagggact tgagtggatt ggatggattt atcctggaag cggtaatact      240

aagtacaatg agaagttcaa gggcaaggcc acattgactg tagacacatc ctccagcaca      300

gccttcatgc agctcagcag cctgacatct gaggacactg ctgtctattt ctgtgcgaac      360

tatggtaact actggtttgc ttactggggc caagggactc aggtcactgt ctctgcagct      420

agc                                                                    423


<210>  29
<211>  432
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA fragment, containing NotI-NheI-flanked VH coding region of 
       the VH domain of anti-human CD19 mAb hBU12

<400>  29
gcggccgcca tgaattttgg actgaggctg attttcctgg tgctgaccct gaaaggcgtc       60

cagtgtcagg tgcagctgca ggaatctggc cctggcctcg tgaagccttc ccagaccctg      120

tctctgacct gcaccgtgtc cggcggctcc atctctacct ctggcatggg cgtgggctgg      180

atcagacagc atcctggcaa gggcctggaa tggatcggcc acatttggtg ggacgacgac      240

aagcggtaca accccgccct gaagtccaga gtgaccatct ccgtggacac ctccaagaac      300

cagttctccc tgaagctgtc ctccgtgacc gccgctgata ccgccgtgta ctactgcgcc      360

cggatggaac tgtggtccta ctacttcgac tactggggcc agggcaccct cgtgaccgtg      420

tcatctgcta gc                                                          432


<210>  30
<211>  733
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA fragment containing IgL coding region of anti-CD30 mAb Ac10 
       and flanked by NotI and BstBI restriction enzyme sites

<400>  30
gcggccgcca tgaattttgg actgaggctg attttcctgg tgctgaccct gaaaggcgtc       60

cagtgtgaca ttgtgctgac ccaatctcca gcttctttgg ctgtgtctct agggcagagg      120

gccaccatct cctgcaaggc cagccaaagt gttgattttg atggtgatag ttatatgaac      180

tggtaccaac agaaaccagg acagccaccc aaagtcctca tctatgctgc atccaatcta      240

gaatctggga tcccagccag gtttagtggc agtgggtctg ggacagactt caccctcaac      300

atccatcctg tggaggagga ggatgctgca acctattact gtcagcaaag taatgaggat      360

ccgtggacgt tcggtggagg caccaagctg gaaatcaaac gtacggtggc tgcaccatct      420

gtcttcatct tcccgccatc tgatgagcag ttgaaatctg gaactgcctc tgttgtgtgc      480

ctgctgaata acttctatcc cagagaggcc aaagtacagt ggaaggtgga taacgccctc      540

caatcgggta actcccagga gagtgtcaca gagcaggaca gcaaggacag cacctacagc      600

ctcagcagca ccctgacgct gagcaaagca gactacgaga aacacaaagt ctacgcctgc      660

gaagtcaccc atcagggcct gagctcgccc gtcacaaaga gcttcaacag gggagagtgt      720

tagcgctttc gaa                                                         733


<210>  31
<211>  718
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA fragment containing IgL coding region of anti-CD19 mAb hBU12 
       and flanked by NotI and BstBI restriction enzyme sites

<400>  31
gcggccgcca tgaattttgg actgaggctg attttcctgg tgctgaccct gaaaggcgtc       60

cagtgtgaaa ttgttctcac ccagtctcca gcaaccctgt ctctctctcc aggggaaagg      120

gctaccctga gctgcagtgc cagctcaagt gtaagttaca tgcactggta ccagcagaag      180

ccagggcagg ctcccagact cctgatttat gacacatcca aactggcttc tggtattcca      240

gcaaggttca gtggcagtgg gtctggaaca gattttacac tcacaatcag cagcctggag      300

ccagaggatg ttgctgtcta ttactgtttt caggggagtg tatacccatt cacttttggc      360

caagggacaa agttggaaat caaacgtacg gtggctgcac catctgtctt catcttcccg      420

ccatctgatg agcagttgaa atctggaact gcctctgttg tgtgcctgct gaataacttc      480

tatcccagag aggccaaagt acagtggaag gtggataacg ccctccaatc gggtaactcc      540

caggagagtg tcacagagca ggacagcaag gacagcacct acagcctcag cagcaccctg      600

acgctgagca aagcagacta cgagaaacac aaagtctacg cctgcgaagt cacccatcag      660

ggcctgagct cgcccgtcac aaagagcttc aacaggggag agtgttagcg ctttcgaa        718


<210>  32
<211>  7645
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence of pPB-EGFP-HC-Ac10

<400>  32
gaattcttaa ccctagaaag atagtctgcg taaaattgac gcatgcattc ttgaaatatt       60

gctctctctt tctaaatagc gcgaatccgt cgctgtgcat ttaggacatc tcagtcgccg      120

cttggagctc ccgtgaggcg tgcttgtcaa tgcggtaagt gtcactgatt ttgaactata      180

acgaccgcgt gagtcaaaat gacgcatgat tatcttttac gtgactttta agatttaact      240

catacgataa ttatattgtt atttcatgtt ctacttacgt gataacttat tatatatata      300

ttttcttgtt atacctcgag gctagtctag agagtaattc atacaaaagg actcgcccct      360

gccttgggga atcccaggga ccgtcgttaa actcccacta acgtagaacc cagagatcgc      420

tgcgttcccg ccccctcacc cgcccgctct cgtcatcact gaggtggaga agagcatgcg      480

tgaggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg      540

gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa      600

agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt      660

gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt      720

gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga      780

attacttcca cgcccctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag      840

tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga      900

ggcctggctt gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct      960

cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt     1020

tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt     1080

tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg     1140

cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct     1200

ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc     1260

ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa     1320

atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc     1380

ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca     1440

cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta     1500

tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt     1560

gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc     1620

tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgagcgg ccgccatgaa     1680

ttttggactg aggctgattt tcctggtgct gaccctgaaa ggcgtccagt gtcagatcca     1740

gctgcagcag tctggccccg aggtcgtgaa acctggcgcc tccgtgaaga tctcctgcaa     1800

ggcctccggc tacaccttca ccgactacta catcacctgg gtcaagcaga agcccggcca     1860

gggcctggaa tggatcggct ggatctatcc cggctccggc aacaccaagt acaacgagaa     1920

gttcaagggc aaggccaccc tgaccgtgga cacctcctct tccaccgcct tcatgcagct     1980

gtcctccctg acctccgagg ataccgccgt gtacttctgc gccaactacg gcaactattg     2040

gttcgcctac tggggccagg gcacacaagt gaccgtgtct gctgctagca ccaagggccc     2100

atcggtcttc cccctggcac cctcctccaa gagcacctct gggggcacag cagccctggg     2160

ctgcctggtc aaggactact tccccgaacc ggtgacggtg tcgtggaact caggcgccct     2220

gaccagcggc gtgcacacct tcccggctgt cctacagtcc tcaggactct actccctcag     2280

cagcgtggtg accgtgccct ccagcagctt gggcacccag acctacatct gcaacgtgaa     2340

tcacaagccc agcaacacca aggtggacaa gaaagttgag cccaaatctt gtgacaaaac     2400

tcacacatgc ccaccgtgcc cagcacctga actcctgggg ggaccgtcag tcttcctctt     2460

ccccccaaaa cccaaggaca ccctcatgat ctcccggacc cctgaggtca catgcgtggt     2520

ggtggacgtg agccacgaag accctgaggt caagttcaac tggtacgtgg acggcgtgga     2580

ggtgcataat gccaagacaa agccgcggga ggagcagtac aacagcacgt accgtgtggt     2640

cagcgtcctc accgtcctgc accaggactg gctgaatggc aaggagtaca agtgcaaggt     2700

ctccaacaaa gccctcccag cccccatcga gaaaaccatc tccaaagcca aagggcagcc     2760

ccgagaacca caggtgtaca ccctgccccc atcccgggat gagctgacca agaaccaggt     2820

cagcctgacc tgcctggtca aaggcttcta tcccagcgac atcgccgtgg agtgggagag     2880

caatgggcag ccggagaaca actacaagac cacgcctccc gtgctggact ccgacggctc     2940

cttcttcctc tacagcaagc tcaccgtgga caagagcagg tggcagcagg ggaacgtctt     3000

ctcatgctcc gtgatgcatg aggctctgca caaccactac acacagaaga gcctctccct     3060

gtctccggag ctgcaactgg aggagagctg tgcggaggcg caggacgggg agctggacgg     3120

gctgtggacg accatcacca tcttcatcac actcttcctg ttaagcgtgt gctacagtgc     3180

caccgtcacc ttcttcaagg tgaagtggat cttctcctcg gtggtggacc tgaagcagac     3240

catcatcccc gactacagga acatgatcgg acagggggcc tagtaagctt agcgctttcg     3300

aagggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct     3360

tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc     3420

actttgcctt tctctccaca ggtgtcctac gtagcgatcg ccaattccgc ccctctccct     3480

cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct     3540

atatgtgatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc     3600

ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc     3660

tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg     3720

tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa     3780

agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt     3840

ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg     3900

atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta     3960

catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt     4020

tcctttgaaa aacacgatga taagcttgcc acaacccggt ttaaacggat cccgccacca     4080

tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg     4140

gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg     4200

gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc     4260

tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc     4320

agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct     4380

tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg     4440

tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca     4500

agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg     4560

gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg     4620

accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact     4680

acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc     4740

tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaaa     4800

gcggcatatg cgccggcgga tatccggcgc gccgagctcg ctgatcagcc tcgactgtgc     4860

ctctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg     4920

tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag     4980

gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga     5040

caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag     5100

ctggggttaa ttaaacgcgt ttaaccctag aaagataatc atattgtgac gtacgttaaa     5160

gataatcatg cgtaaaattg acgcatgtgt tttatcggtc tgtatatcga ggtttattta     5220

ttaatttgaa tagatattaa gttttattat atttacactt acatactaat aataaattca     5280

acaaacaatt tatttatgtt tatttattta ttaaaaaaaa acaaaaactc aaaatttctt     5340

ctataaagta acaaaacttt tatatcgatt cgcgcgcgtg cattctagtt gtggtttgtc     5400

caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc     5460

gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa     5520

catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac     5580

attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca     5640

ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc     5700

ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc     5760

aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc     5820

aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag     5880

gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc     5940

gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt     6000

tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct     6060

ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg     6120

ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct     6180

tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat     6240

tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg     6300

ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa     6360

aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt     6420

ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc     6480

tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt     6540

atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta     6600

aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat     6660

ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac     6720

tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg     6780

ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag     6840

tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt     6900

aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt     6960

gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt     7020

tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt     7080

cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct     7140

tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt     7200

ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac     7260

cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa     7320

actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa     7380

ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca     7440

aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct     7500

ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga     7560

atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc     7620

tgacgtcgac ggatcgggcc tgcag                                           7645


<210>  33
<211>  7654
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence of pPB-EGFP-HC-hBU12

<400>  33
gaattcttaa ccctagaaag atagtctgcg taaaattgac gcatgcattc ttgaaatatt       60

gctctctctt tctaaatagc gcgaatccgt cgctgtgcat ttaggacatc tcagtcgccg      120

cttggagctc ccgtgaggcg tgcttgtcaa tgcggtaagt gtcactgatt ttgaactata      180

acgaccgcgt gagtcaaaat gacgcatgat tatcttttac gtgactttta agatttaact      240

catacgataa ttatattgtt atttcatgtt ctacttacgt gataacttat tatatatata      300

ttttcttgtt atacctcgag gctagtctag agagtaattc atacaaaagg actcgcccct      360

gccttgggga atcccaggga ccgtcgttaa actcccacta acgtagaacc cagagatcgc      420

tgcgttcccg ccccctcacc cgcccgctct cgtcatcact gaggtggaga agagcatgcg      480

tgaggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg      540

gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa      600

agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt      660

gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt      720

gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga      780

attacttcca cgcccctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag      840

tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga      900

ggcctggctt gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct      960

cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt     1020

tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt     1080

tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg     1140

cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct     1200

ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc     1260

ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa     1320

atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc     1380

ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca     1440

cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta     1500

tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt     1560

gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc     1620

tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgagcgg ccgccatgaa     1680

ttttggactg aggctgattt tcctggtgct gaccctgaaa ggcgtccagt gtcaggtgca     1740

gctgcaggaa tctggccctg gcctcgtgaa gccttcccag accctgtctc tgacctgcac     1800

cgtgtccggc ggctccatct ctacctctgg catgggcgtg ggctggatca gacagcatcc     1860

tggcaagggc ctggaatgga tcggccacat ttggtgggac gacgacaagc ggtacaaccc     1920

cgccctgaag tccagagtga ccatctccgt ggacacctcc aagaaccagt tctccctgaa     1980

gctgtcctcc gtgaccgccg ctgataccgc cgtgtactac tgcgcccgga tggaactgtg     2040

gtcctactac ttcgactact ggggccaggg caccctcgtg accgtgtcat ctgctagcac     2100

caagggccca tcggtcttcc ccctggcacc ctcctccaag agcacctctg ggggcacagc     2160

agccctgggc tgcctggtca aggactactt ccccgaaccg gtgacggtgt cgtggaactc     2220

aggcgccctg accagcggcg tgcacacctt cccggctgtc ctacagtcct caggactcta     2280

ctccctcagc agcgtggtga ccgtgccctc cagcagcttg ggcacccaga cctacatctg     2340

caacgtgaat cacaagccca gcaacaccaa ggtggacaag aaagttgagc ccaaatcttg     2400

tgacaaaact cacacatgcc caccgtgccc agcacctgaa ctcctggggg gaccgtcagt     2460

cttcctcttc cccccaaaac ccaaggacac cctcatgatc tcccggaccc ctgaggtcac     2520

atgcgtggtg gtggacgtga gccacgaaga ccctgaggtc aagttcaact ggtacgtgga     2580

cggcgtggag gtgcataatg ccaagacaaa gccgcgggag gagcagtaca acagcacgta     2640

ccgtgtggtc agcgtcctca ccgtcctgca ccaggactgg ctgaatggca aggagtacaa     2700

gtgcaaggtc tccaacaaag ccctcccagc ccccatcgag aaaaccatct ccaaagccaa     2760

agggcagccc cgagaaccac aggtgtacac cctgccccca tcccgggatg agctgaccaa     2820

gaaccaggtc agcctgacct gcctggtcaa aggcttctat cccagcgaca tcgccgtgga     2880

gtgggagagc aatgggcagc cggagaacaa ctacaagacc acgcctcccg tgctggactc     2940

cgacggctcc ttcttcctct acagcaagct caccgtggac aagagcaggt ggcagcaggg     3000

gaacgtcttc tcatgctccg tgatgcatga ggctctgcac aaccactaca cacagaagag     3060

cctctccctg tctccggagc tgcaactgga ggagagctgt gcggaggcgc aggacgggga     3120

gctggacggg ctgtggacga ccatcaccat cttcatcaca ctcttcctgt taagcgtgtg     3180

ctacagtgcc accgtcacct tcttcaaggt gaagtggatc ttctcctcgg tggtggacct     3240

gaagcagacc atcatccccg actacaggaa catgatcgga cagggggcct agtaagctta     3300

gcgctttcga agggcaggta agtatcaagg ttacaagaca ggtttaagga gaccaataga     3360

aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta     3420

ctgacatcca ctttgccttt ctctccacag gtgtcctacg tagcgatcgc caattccgcc     3480

cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg     3540

cgtttgtcta tatgtgattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga     3600

aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa     3660

tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa     3720

caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct     3780

gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg     3840

ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg     3900

ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca     3960

catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg     4020

acgtggtttt cctttgaaaa acacgatgat aagcttgcca caacccggtt taaacggatc     4080

ccgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg     4140

agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg     4200

ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct     4260

ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc     4320

acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca     4380

ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg     4440

acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc     4500

tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc     4560

agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc     4620

agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg     4680

acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc     4740

acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt     4800

acaagtaaag cggcatatgc gccggcggat atccggcgcg ccgagctcgc tgatcagcct     4860

cgactgtgcc tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac     4920

cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg     4980

tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga     5040

ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga     5100

aagaaccagc tggggttaat taaacgcgtt taaccctaga aagataatca tattgtgacg     5160

tacgttaaag ataatcatgc gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag     5220

gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata     5280

ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaaa caaaaactca     5340

aaatttcttc tataaagtaa caaaactttt atatcgattc gcgcgcgtgc attctagttg     5400

tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta     5460

gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat     5520

tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag     5580

ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg     5640

ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc     5700

ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc     5760

agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa     5820

catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt     5880

tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg     5940

gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg     6000

ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag     6060

cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc     6120

caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa     6180

ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg     6240

taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc     6300

taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac     6360

cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg     6420

tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt     6480

gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt     6540

catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa     6600

atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga     6660

ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt     6720

gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg     6780

agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga     6840

gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga     6900

agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg     6960

catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc     7020

aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc     7080

gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca     7140

taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac     7200

caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg     7260

ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc     7320

ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg     7380

tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac     7440

aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat     7500

actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata     7560

catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa     7620

agtgccacct gacgtcgacg gatcgggcct gcag                                 7654


<210>  34
<211>  7549
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence of pSB-EGFP-HC-Ac10

<400>  34
gaattcagtt gaagtcggaa gtttacatac acttaagttg gagtcattaa aactcgtttt       60

tcaactacac cacaaatttc ttgttaacaa acaatagttt tggcaagtca gttaggacat      120

ctactttgtg catgacacaa gtcatttttc caacaattgt ttacagacag attatttcac      180

ttataattca ctgtatcaca attccagtgg gtcagaagtt tacatacact aacctcgagg      240

ctagtctaga gagtaattca tacaaaagga ctcgcccctg ccttggggaa tcccagggac      300

cgtcgttaaa ctcccactaa cgtagaaccc agagatcgct gcgttcccgc cccctcaccc      360

gcccgctctc gtcatcactg aggtggagaa gagcatgcgt gaggctccgg tgcccgtcag      420

tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga      480

accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc      540

cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt      600

ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg      660

cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac gcccctggct      720

gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc      780

ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcttg ggcgctgggg      840

ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct      900

agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt      960

aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg     1020

ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag     1080

aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc     1140

gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga     1200

aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg     1260

agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc     1320

ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt     1380

ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac     1440

tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt     1500

tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt     1560

ttttcttcca tttcaggtgt cgtgagcggc cgccatgaat tttggactga ggctgatttt     1620

cctggtgctg accctgaaag gcgtccagtg tcagatccag ctgcagcagt ctggccccga     1680

ggtcgtgaaa cctggcgcct ccgtgaagat ctcctgcaag gcctccggct acaccttcac     1740

cgactactac atcacctggg tcaagcagaa gcccggccag ggcctggaat ggatcggctg     1800

gatctatccc ggctccggca acaccaagta caacgagaag ttcaagggca aggccaccct     1860

gaccgtggac acctcctctt ccaccgcctt catgcagctg tcctccctga cctccgagga     1920

taccgccgtg tacttctgcg ccaactacgg caactattgg ttcgcctact ggggccaggg     1980

cacacaagtg accgtgtctg ctgctagcac caagggccca tcggtcttcc ccctggcacc     2040

ctcctccaag agcacctctg ggggcacagc agccctgggc tgcctggtca aggactactt     2100

ccccgaaccg gtgacggtgt cgtggaactc aggcgccctg accagcggcg tgcacacctt     2160

cccggctgtc ctacagtcct caggactcta ctccctcagc agcgtggtga ccgtgccctc     2220

cagcagcttg ggcacccaga cctacatctg caacgtgaat cacaagccca gcaacaccaa     2280

ggtggacaag aaagttgagc ccaaatcttg tgacaaaact cacacatgcc caccgtgccc     2340

agcacctgaa ctcctggggg gaccgtcagt cttcctcttc cccccaaaac ccaaggacac     2400

cctcatgatc tcccggaccc ctgaggtcac atgcgtggtg gtggacgtga gccacgaaga     2460

ccctgaggtc aagttcaact ggtacgtgga cggcgtggag gtgcataatg ccaagacaaa     2520

gccgcgggag gagcagtaca acagcacgta ccgtgtggtc agcgtcctca ccgtcctgca     2580

ccaggactgg ctgaatggca aggagtacaa gtgcaaggtc tccaacaaag ccctcccagc     2640

ccccatcgag aaaaccatct ccaaagccaa agggcagccc cgagaaccac aggtgtacac     2700

cctgccccca tcccgggatg agctgaccaa gaaccaggtc agcctgacct gcctggtcaa     2760

aggcttctat cccagcgaca tcgccgtgga gtgggagagc aatgggcagc cggagaacaa     2820

ctacaagacc acgcctcccg tgctggactc cgacggctcc ttcttcctct acagcaagct     2880

caccgtggac aagagcaggt ggcagcaggg gaacgtcttc tcatgctccg tgatgcatga     2940

ggctctgcac aaccactaca cacagaagag cctctccctg tctccggagc tgcaactgga     3000

ggagagctgt gcggaggcgc aggacgggga gctggacggg ctgtggacga ccatcaccat     3060

cttcatcaca ctcttcctgt taagcgtgtg ctacagtgcc accgtcacct tcttcaaggt     3120

gaagtggatc ttctcctcgg tggtggacct gaagcagacc atcatccccg actacaggaa     3180

catgatcgga cagggggcct agtaagctta gcgctttcga agggcaggta agtatcaagg     3240

ttacaagaca ggtttaagga gaccaataga aactgggctt gtcgagacag agaagactct     3300

tgcgtttctg ataggcacct attggtctta ctgacatcca ctttgccttt ctctccacag     3360

gtgtcctacg tagcgatcgc caattccgcc cctctccctc ccccccccct aacgttactg     3420

gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgtgattt tccaccatat     3480

tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc     3540

ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag     3600

cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc     3660

ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac     3720

ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca     3780

aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt     3840

gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa     3900

aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat     3960

aagcttgcca caacccggtt taaacggatc ccgccaccat ggtgagcaag ggcgaggagc     4020

tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt     4080

tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca     4140

tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg     4200

gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg     4260

ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca     4320

agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg     4380

gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca     4440

gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga     4500

tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc     4560

ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc     4620

tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg     4680

ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggcatatgc gccggcggat     4740

atccggcgcg ccgagctcgc tgatcagcct cgactgtgcc tctagttgcc agccatctgt     4800

tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc     4860

ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg     4920

tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga     4980

tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggttaat taaacgcgtt     5040

tgagtgtatg ttaacttctg acccactggg aatgtgatga aagaaataaa agctgaaatg     5100

aatcattctc tctactatta ttctgatatt tcacattctt aaaataaagt ggtgatccta     5160

actgacctta agacagggaa tctttactcg gattaaatgt caggaattgt gaaaaagtga     5220

gtttaaatgt atttggctaa ggtgtatgta aacttccgac ttcaactatc gattcgcgcg     5280

cgtgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc     5340

gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg     5400

ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg     5460

tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc     5520

gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt     5580

gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct     5640

gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga     5700

taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc     5760

cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg     5820

ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg     5880

aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt     5940

tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt     6000

gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg     6060

cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact     6120

ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt     6180

cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct     6240

gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac     6300

cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc     6360

tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg     6420

ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta     6480

aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca     6540

atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc     6600

ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc     6660

tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc     6720

agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat     6780

taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt     6840

tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc     6900

cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag     6960

ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt     7020

tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac     7080

tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg     7140

cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat     7200

tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc     7260

gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc     7320

tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa     7380

atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg     7440

tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg     7500

cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggcctgcag                 7549


<210>  35
<211>  7558
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence of pSB-EGFP-HC-hBU12

<400>  35
gaattcagtt gaagtcggaa gtttacatac acttaagttg gagtcattaa aactcgtttt       60

tcaactacac cacaaatttc ttgttaacaa acaatagttt tggcaagtca gttaggacat      120

ctactttgtg catgacacaa gtcatttttc caacaattgt ttacagacag attatttcac      180

ttataattca ctgtatcaca attccagtgg gtcagaagtt tacatacact aacctcgagg      240

ctagtctaga gagtaattca tacaaaagga ctcgcccctg ccttggggaa tcccagggac      300

cgtcgttaaa ctcccactaa cgtagaaccc agagatcgct gcgttcccgc cccctcaccc      360

gcccgctctc gtcatcactg aggtggagaa gagcatgcgt gaggctccgg tgcccgtcag      420

tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga      480

accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc      540

cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt      600

ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg      660

cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac gcccctggct      720

gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc      780

ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcttg ggcgctgggg      840

ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct      900

agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt      960

aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg     1020

ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag     1080

aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc     1140

gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga     1200

aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg     1260

agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc     1320

ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt     1380

ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac     1440

tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt     1500

tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt     1560

ttttcttcca tttcaggtgt cgtgagcggc cgccatgaat tttggactga ggctgatttt     1620

cctggtgctg accctgaaag gcgtccagtg tcaggtgcag ctgcaggaat ctggccctgg     1680

cctcgtgaag ccttcccaga ccctgtctct gacctgcacc gtgtccggcg gctccatctc     1740

tacctctggc atgggcgtgg gctggatcag acagcatcct ggcaagggcc tggaatggat     1800

cggccacatt tggtgggacg acgacaagcg gtacaacccc gccctgaagt ccagagtgac     1860

catctccgtg gacacctcca agaaccagtt ctccctgaag ctgtcctccg tgaccgccgc     1920

tgataccgcc gtgtactact gcgcccggat ggaactgtgg tcctactact tcgactactg     1980

gggccagggc accctcgtga ccgtgtcatc tgctagcacc aagggcccat cggtcttccc     2040

cctggcaccc tcctccaaga gcacctctgg gggcacagca gccctgggct gcctggtcaa     2100

ggactacttc cccgaaccgg tgacggtgtc gtggaactca ggcgccctga ccagcggcgt     2160

gcacaccttc ccggctgtcc tacagtcctc aggactctac tccctcagca gcgtggtgac     2220

cgtgccctcc agcagcttgg gcacccagac ctacatctgc aacgtgaatc acaagcccag     2280

caacaccaag gtggacaaga aagttgagcc caaatcttgt gacaaaactc acacatgccc     2340

accgtgccca gcacctgaac tcctgggggg accgtcagtc ttcctcttcc ccccaaaacc     2400

caaggacacc ctcatgatct cccggacccc tgaggtcaca tgcgtggtgg tggacgtgag     2460

ccacgaagac cctgaggtca agttcaactg gtacgtggac ggcgtggagg tgcataatgc     2520

caagacaaag ccgcgggagg agcagtacaa cagcacgtac cgtgtggtca gcgtcctcac     2580

cgtcctgcac caggactggc tgaatggcaa ggagtacaag tgcaaggtct ccaacaaagc     2640

cctcccagcc cccatcgaga aaaccatctc caaagccaaa gggcagcccc gagaaccaca     2700

ggtgtacacc ctgcccccat cccgggatga gctgaccaag aaccaggtca gcctgacctg     2760

cctggtcaaa ggcttctatc ccagcgacat cgccgtggag tgggagagca atgggcagcc     2820

ggagaacaac tacaagacca cgcctcccgt gctggactcc gacggctcct tcttcctcta     2880

cagcaagctc accgtggaca agagcaggtg gcagcagggg aacgtcttct catgctccgt     2940

gatgcatgag gctctgcaca accactacac acagaagagc ctctccctgt ctccggagct     3000

gcaactggag gagagctgtg cggaggcgca ggacggggag ctggacgggc tgtggacgac     3060

catcaccatc ttcatcacac tcttcctgtt aagcgtgtgc tacagtgcca ccgtcacctt     3120

cttcaaggtg aagtggatct tctcctcggt ggtggacctg aagcagacca tcatccccga     3180

ctacaggaac atgatcggac agggggccta gtaagcttag cgctttcgaa gggcaggtaa     3240

gtatcaaggt tacaagacag gtttaaggag accaatagaa actgggcttg tcgagacaga     3300

gaagactctt gcgtttctga taggcaccta ttggtcttac tgacatccac tttgcctttc     3360

tctccacagg tgtcctacgt agcgatcgcc aattccgccc ctctccctcc ccccccccta     3420

acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgtgatttt     3480

ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga     3540

cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg     3600

tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt     3660

gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat     3720

aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg     3780

aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg     3840

taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt     3900

cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa     3960

cacgatgata agcttgccac aacccggttt aaacggatcc cgccaccatg gtgagcaagg     4020

gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg     4080

gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc     4140

tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc     4200

tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct     4260

tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg     4320

gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg     4380

agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca     4440

actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga     4500

acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc     4560

agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc     4620

agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg     4680

tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaagc ggcatatgcg     4740

ccggcggata tccggcgcgc cgagctcgct gatcagcctc gactgtgcct ctagttgcca     4800

gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac     4860

tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat     4920

tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca     4980

tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggttaatt     5040

aaacgcgttt gagtgtatgt taacttctga cccactggga atgtgatgaa agaaataaaa     5100

gctgaaatga atcattctct ctactattat tctgatattt cacattctta aaataaagtg     5160

gtgatcctaa ctgaccttaa gacagggaat ctttactcgg attaaatgtc aggaattgtg     5220

aaaaagtgag tttaaatgta tttggctaag gtgtatgtaa acttccgact tcaactatcg     5280

attcgcgcgc gtgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt     5340

ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt     5400

gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa     5460

agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc     5520

tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag     5580

aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt     5640

cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga     5700

atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg     5760

taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa     5820

aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt     5880

tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct     5940

gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct     6000

cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc     6060

cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt     6120

atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc     6180

tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat     6240

ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa     6300

acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa     6360

aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga     6420

aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct     6480

tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga     6540

cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc     6600

catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg     6660

ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat     6720

aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat     6780

ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg     6840

caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc     6900

attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa     6960

agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc     7020

actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt     7080

ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag     7140

ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt     7200

gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag     7260

atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac     7320

cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc     7380

gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca     7440

gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg     7500

ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gcctgcag       7558


<210>  36
<211>  6742
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence of pPB-EGFP-LC-Ac10

<400>  36
gaattcttaa ccctagaaag atagtctgcg taaaattgac gcatgcattc ttgaaatatt       60

gctctctctt tctaaatagc gcgaatccgt cgctgtgcat ttaggacatc tcagtcgccg      120

cttggagctc ccgtgaggcg tgcttgtcaa tgcggtaagt gtcactgatt ttgaactata      180

acgaccgcgt gagtcaaaat gacgcatgat tatcttttac gtgactttta agatttaact      240

catacgataa ttatattgtt atttcatgtt ctacttacgt gataacttat tatatatata      300

ttttcttgtt atacctcgag gctagtctag agagtaattc atacaaaagg actcgcccct      360

gccttgggga atcccaggga ccgtcgttaa actcccacta acgtagaacc cagagatcgc      420

tgcgttcccg ccccctcacc cgcccgctct cgtcatcact gaggtggaga agagcatgcg      480

tgaggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg      540

gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa      600

agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt      660

gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt      720

gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga      780

attacttcca cgcccctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag      840

tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga      900

ggcctggctt gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct      960

cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt     1020

tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt     1080

tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg     1140

cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct     1200

ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc     1260

ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa     1320

atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc     1380

ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca     1440

cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta     1500

tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt     1560

gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc     1620

tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgagcgg ccgccatgaa     1680

ttttggactg aggctgattt tcctggtgct gaccctgaaa ggcgtccagt gtgacattgt     1740

gctgacccaa tctccagctt ctttggctgt gtctctaggg cagagggcca ccatctcctg     1800

caaggccagc caaagtgttg attttgatgg tgatagttat atgaactggt accaacagaa     1860

accaggacag ccacccaaag tcctcatcta tgctgcatcc aatctagaat ctgggatccc     1920

agccaggttt agtggcagtg ggtctgggac agacttcacc ctcaacatcc atcctgtgga     1980

ggaggaggat gctgcaacct attactgtca gcaaagtaat gaggatccgt ggacgttcgg     2040

tggaggcacc aagctggaaa tcaaacgtac ggtggctgca ccatctgtct tcatcttccc     2100

gccatctgat gagcagttga aatctggaac tgcctctgtt gtgtgcctgc tgaataactt     2160

ctatcccaga gaggccaaag tacagtggaa ggtggataac gccctccaat cgggtaactc     2220

ccaggagagt gtcacagagc aggacagcaa ggacagcacc tacagcctca gcagcaccct     2280

gacgctgagc aaagcagact acgagaaaca caaagtctac gcctgcgaag tcacccatca     2340

gggcctgagc tcgcccgtca caaagagctt caacagggga gagtgttagc gctttcgaag     2400

ggcaggtaag tatcaaggtt acaagacagg tttaaggaga ccaatagaaa ctgggcttgt     2460

cgagacagag aagactcttg cgtttctgat aggcacctat tggtcttact gacatccact     2520

ttgcctttct ctccacaggt gtcctacgta gcgatcgcca attccgcccc tctccctccc     2580

ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata     2640

tgtgattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg     2700

tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt     2760

tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag     2820

cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc     2880

cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga     2940

tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg     3000

cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat     3060

gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc     3120

tttgaaaaac acgatgataa gcttgccaca acccggttta aacggatccc gccaccatgg     3180

tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg     3240

acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca     3300

agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg     3360

tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc     3420

acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca     3480

aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga     3540

accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc     3600

tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca     3660

tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc     3720

actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc     3780

tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc     3840

tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg     3900

gcatatgcgc cggcggatat ccggcgcgcc gagctcgctg atcagcctcg actgtgcctc     3960

tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc     4020

cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg     4080

tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa     4140

tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg     4200

gggttaatta aacgcgttta accctagaaa gataatcata ttgtgacgta cgttaaagat     4260

aatcatgcgt aaaattgacg catgtgtttt atcggtctgt atatcgaggt ttatttatta     4320

atttgaatag atattaagtt ttattatatt tacacttaca tactaataat aaattcaaca     4380

aacaatttat ttatgtttat ttatttatta aaaaaaaaca aaaactcaaa atttcttcta     4440

taaagtaaca aaacttttat atcgattcgc gcgcgtgcat tctagttgtg gtttgtccaa     4500

actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta     4560

atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat     4620

acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt     4680

aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta     4740

atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc     4800

gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa     4860

ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa     4920

aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct     4980

ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac     5040

aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc     5100

gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc     5160

tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg     5220

tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga     5280

gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag     5340

cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta     5400

cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag     5460

agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg     5520

caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac     5580

ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc     5640

aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag     5700

tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc     5760

agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac     5820

gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc     5880

accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg     5940

tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag     6000

tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc     6060

acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac     6120

atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag     6180

aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac     6240

tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg     6300

agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc     6360

gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact     6420

ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg     6480

atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa     6540

tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt     6600

tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg     6660

tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga     6720

cgtcgacgga tcgggcctgc ag                                              6742


<210>  37
<211>  6727
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence of pPB-EGFP-LC-hBU12

<400>  37
gaattcttaa ccctagaaag atagtctgcg taaaattgac gcatgcattc ttgaaatatt       60

gctctctctt tctaaatagc gcgaatccgt cgctgtgcat ttaggacatc tcagtcgccg      120

cttggagctc ccgtgaggcg tgcttgtcaa tgcggtaagt gtcactgatt ttgaactata      180

acgaccgcgt gagtcaaaat gacgcatgat tatcttttac gtgactttta agatttaact      240

catacgataa ttatattgtt atttcatgtt ctacttacgt gataacttat tatatatata      300

ttttcttgtt atacctcgag gctagtctag agagtaattc atacaaaagg actcgcccct      360

gccttgggga atcccaggga ccgtcgttaa actcccacta acgtagaacc cagagatcgc      420

tgcgttcccg ccccctcacc cgcccgctct cgtcatcact gaggtggaga agagcatgcg      480

tgaggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg      540

gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa      600

agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt      660

gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt      720

gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga      780

attacttcca cgcccctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag      840

tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga      900

ggcctggctt gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct      960

cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt     1020

tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt     1080

tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg     1140

cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct     1200

ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc     1260

ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa     1320

atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc     1380

ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca     1440

cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta     1500

tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt     1560

gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc     1620

tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgagcgg ccgccatgaa     1680

ttttggactg aggctgattt tcctggtgct gaccctgaaa ggcgtccagt gtgaaattgt     1740

tctcacccag tctccagcaa ccctgtctct ctctccaggg gaaagggcta ccctgagctg     1800

cagtgccagc tcaagtgtaa gttacatgca ctggtaccag cagaagccag ggcaggctcc     1860

cagactcctg atttatgaca catccaaact ggcttctggt attccagcaa ggttcagtgg     1920

cagtgggtct ggaacagatt ttacactcac aatcagcagc ctggagccag aggatgttgc     1980

tgtctattac tgttttcagg ggagtgtata cccattcact tttggccaag ggacaaagtt     2040

ggaaatcaaa cgtacggtgg ctgcaccatc tgtcttcatc ttcccgccat ctgatgagca     2100

gttgaaatct ggaactgcct ctgttgtgtg cctgctgaat aacttctatc ccagagaggc     2160

caaagtacag tggaaggtgg ataacgccct ccaatcgggt aactcccagg agagtgtcac     2220

agagcaggac agcaaggaca gcacctacag cctcagcagc accctgacgc tgagcaaagc     2280

agactacgag aaacacaaag tctacgcctg cgaagtcacc catcagggcc tgagctcgcc     2340

cgtcacaaag agcttcaaca ggggagagtg ttagcgcttt cgaagggcag gtaagtatca     2400

aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga cagagaagac     2460

tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc tttctctcca     2520

caggtgtcct acgtagcgat cgccaattcc gcccctctcc ctcccccccc cctaacgtta     2580

ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtga ttttccacca     2640

tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca     2700

ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg     2760

aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc     2820

agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata     2880

cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag     2940

tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc     3000

attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt     3060

taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat     3120

gataagcttg ccacaacccg gtttaaacgg atcccgccac catggtgagc aagggcgagg     3180

agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca     3240

agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt     3300

tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct     3360

acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt     3420

ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact     3480

acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga     3540

agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca     3600

acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca     3660

agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca     3720

cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg     3780

ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg     3840

ccgccgggat cactctcggc atggacgagc tgtacaagta aagcggcata tgcgccggcg     3900

gatatccggc gcgccgagct cgctgatcag cctcgactgt gcctctagtt gccagccatc     3960

tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct     4020

ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg     4080

gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg     4140

ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggtt aattaaacgc     4200

gtttaaccct agaaagataa tcatattgtg acgtacgtta aagataatca tgcgtaaaat     4260

tgacgcatgt gttttatcgg tctgtatatc gaggtttatt tattaatttg aatagatatt     4320

aagttttatt atatttacac ttacatacta ataataaatt caacaaacaa tttatttatg     4380

tttatttatt tattaaaaaa aaacaaaaac tcaaaatttc ttctataaag taacaaaact     4440

tttatatcga ttcgcgcgcg tgcattctag ttgtggtttg tccaaactca tcaatgtatc     4500

ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct     4560

gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat     4620

aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc     4680

actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg     4740

cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct     4800

gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt     4860

atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc     4920

caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga     4980

gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata     5040

ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac     5100

cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg     5160

taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc     5220

cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag     5280

acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt     5340

aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt     5400

atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg     5460

atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac     5520

gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca     5580

gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac     5640

ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac     5700

ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt     5760

tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt     5820

accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt     5880

atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc     5940

cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa     6000

tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg     6060

tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt     6120

gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc     6180

agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt     6240

aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg     6300

gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac     6360

tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc     6420

gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt     6480

tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg     6540

aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag     6600

catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa     6660

acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg     6720

cctgcag                                                               6727


<210>  38
<211>  6646
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence of pSB-EGFP-LC-Ac10

<400>  38
gaattcagtt gaagtcggaa gtttacatac acttaagttg gagtcattaa aactcgtttt       60

tcaactacac cacaaatttc ttgttaacaa acaatagttt tggcaagtca gttaggacat      120

ctactttgtg catgacacaa gtcatttttc caacaattgt ttacagacag attatttcac      180

ttataattca ctgtatcaca attccagtgg gtcagaagtt tacatacact aacctcgagg      240

ctagtctaga gagtaattca tacaaaagga ctcgcccctg ccttggggaa tcccagggac      300

cgtcgttaaa ctcccactaa cgtagaaccc agagatcgct gcgttcccgc cccctcaccc      360

gcccgctctc gtcatcactg aggtggagaa gagcatgcgt gaggctccgg tgcccgtcag      420

tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga      480

accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc      540

cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt      600

ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg      660

cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac gcccctggct      720

gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc      780

ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcttg ggcgctgggg      840

ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct      900

agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt      960

aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg     1020

ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag     1080

aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc     1140

gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga     1200

aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg     1260

agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc     1320

ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt     1380

ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac     1440

tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt     1500

tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt     1560

ttttcttcca tttcaggtgt cgtgagcggc cgccatgaat tttggactga ggctgatttt     1620

cctggtgctg accctgaaag gcgtccagtg tgacattgtg ctgacccaat ctccagcttc     1680

tttggctgtg tctctagggc agagggccac catctcctgc aaggccagcc aaagtgttga     1740

ttttgatggt gatagttata tgaactggta ccaacagaaa ccaggacagc cacccaaagt     1800

cctcatctat gctgcatcca atctagaatc tgggatccca gccaggttta gtggcagtgg     1860

gtctgggaca gacttcaccc tcaacatcca tcctgtggag gaggaggatg ctgcaaccta     1920

ttactgtcag caaagtaatg aggatccgtg gacgttcggt ggaggcacca agctggaaat     1980

caaacgtacg gtggctgcac catctgtctt catcttcccg ccatctgatg agcagttgaa     2040

atctggaact gcctctgttg tgtgcctgct gaataacttc tatcccagag aggccaaagt     2100

acagtggaag gtggataacg ccctccaatc gggtaactcc caggagagtg tcacagagca     2160

ggacagcaag gacagcacct acagcctcag cagcaccctg acgctgagca aagcagacta     2220

cgagaaacac aaagtctacg cctgcgaagt cacccatcag ggcctgagct cgcccgtcac     2280

aaagagcttc aacaggggag agtgttagcg ctttcgaagg gcaggtaagt atcaaggtta     2340

caagacaggt ttaaggagac caatagaaac tgggcttgtc gagacagaga agactcttgc     2400

gtttctgata ggcacctatt ggtcttactg acatccactt tgcctttctc tccacaggtg     2460

tcctacgtag cgatcgccaa ttccgcccct ctccctcccc cccccctaac gttactggcc     2520

gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gtgattttcc accatattgc     2580

cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta     2640

ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag     2700

ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga     2760

accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg     2820

caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat     2880

ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta     2940

tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa     3000

aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataag     3060

cttgccacaa cccggtttaa acggatcccg ccaccatggt gagcaagggc gaggagctgt     3120

tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca     3180

gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct     3240

gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg     3300

tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca     3360

tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga     3420

cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca     3480

tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc     3540

acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc     3600

gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca     3660

tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga     3720

gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg     3780

ggatcactct cggcatggac gagctgtaca agtaaagcgg catatgcgcc ggcggatatc     3840

cggcgcgccg agctcgctga tcagcctcga ctgtgcctct agttgccagc catctgttgt     3900

ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta     3960

ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg     4020

ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc     4080

ggtgggctct atggcttctg aggcggaaag aaccagctgg ggttaattaa acgcgtttga     4140

gtgtatgtta acttctgacc cactgggaat gtgatgaaag aaataaaagc tgaaatgaat     4200

cattctctct actattattc tgatatttca cattcttaaa ataaagtggt gatcctaact     4260

gaccttaaga cagggaatct ttactcggat taaatgtcag gaattgtgaa aaagtgagtt     4320

taaatgtatt tggctaaggt gtatgtaaac ttccgacttc aactatcgat tcgcgcgcgt     4380

gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc     4440

gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta     4500

tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc     4560

ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg     4620

aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg     4680

tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg     4740

gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa     4800

cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc     4860

gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc     4920

aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag     4980

ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct     5040

cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta     5100

ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc     5160

cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc     5220

agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt     5280

gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct     5340

gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc     5400

tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca     5460

agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta     5520

agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa     5580

atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg     5640

cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg     5700

actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc     5760

aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc     5820

cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa     5880

ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc     5940

cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg     6000

ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc     6060

cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat     6120

ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg     6180

tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc     6240

ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg     6300

aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat     6360

gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg     6420

gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg     6480

ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct     6540

catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac     6600

atttccccga aaagtgccac ctgacgtcga cggatcgggc ctgcag                    6646


<210>  39
<211>  6631
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence of pSB-EGFP-LC-hBU12

<400>  39
gaattcagtt gaagtcggaa gtttacatac acttaagttg gagtcattaa aactcgtttt       60

tcaactacac cacaaatttc ttgttaacaa acaatagttt tggcaagtca gttaggacat      120

ctactttgtg catgacacaa gtcatttttc caacaattgt ttacagacag attatttcac      180

ttataattca ctgtatcaca attccagtgg gtcagaagtt tacatacact aacctcgagg      240

ctagtctaga gagtaattca tacaaaagga ctcgcccctg ccttggggaa tcccagggac      300

cgtcgttaaa ctcccactaa cgtagaaccc agagatcgct gcgttcccgc cccctcaccc      360

gcccgctctc gtcatcactg aggtggagaa gagcatgcgt gaggctccgg tgcccgtcag      420

tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga      480

accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc      540

cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt      600

ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg      660

cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac gcccctggct      720

gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc      780

ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcttg ggcgctgggg      840

ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct      900

agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt      960

aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg     1020

ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag     1080

aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc     1140

gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga     1200

aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg     1260

agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc     1320

ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt     1380

ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac     1440

tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt     1500

tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt     1560

ttttcttcca tttcaggtgt cgtgagcggc cgccatgaat tttggactga ggctgatttt     1620

cctggtgctg accctgaaag gcgtccagtg tgaaattgtt ctcacccagt ctccagcaac     1680

cctgtctctc tctccagggg aaagggctac cctgagctgc agtgccagct caagtgtaag     1740

ttacatgcac tggtaccagc agaagccagg gcaggctccc agactcctga tttatgacac     1800

atccaaactg gcttctggta ttccagcaag gttcagtggc agtgggtctg gaacagattt     1860

tacactcaca atcagcagcc tggagccaga ggatgttgct gtctattact gttttcaggg     1920

gagtgtatac ccattcactt ttggccaagg gacaaagttg gaaatcaaac gtacggtggc     1980

tgcaccatct gtcttcatct tcccgccatc tgatgagcag ttgaaatctg gaactgcctc     2040

tgttgtgtgc ctgctgaata acttctatcc cagagaggcc aaagtacagt ggaaggtgga     2100

taacgccctc caatcgggta actcccagga gagtgtcaca gagcaggaca gcaaggacag     2160

cacctacagc ctcagcagca ccctgacgct gagcaaagca gactacgaga aacacaaagt     2220

ctacgcctgc gaagtcaccc atcagggcct gagctcgccc gtcacaaaga gcttcaacag     2280

gggagagtgt tagcgctttc gaagggcagg taagtatcaa ggttacaaga caggtttaag     2340

gagaccaata gaaactgggc ttgtcgagac agagaagact cttgcgtttc tgataggcac     2400

ctattggtct tactgacatc cactttgcct ttctctccac aggtgtccta cgtagcgatc     2460

gccaattccg cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat     2520

aaggccggtg tgcgtttgtc tatatgtgat tttccaccat attgccgtct tttggcaatg     2580

tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc     2640

tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt     2700

cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg     2760

acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac     2820

cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg     2880

tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg     2940

ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc     3000

cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataagcttgc cacaacccgg     3060

tttaaacgga tcccgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc     3120

ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg     3180

gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc     3240

tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc     3300

gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg     3360

tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga     3420

agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg     3480

acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca     3540

tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg     3600

acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg     3660

tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg     3720

agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca     3780

tggacgagct gtacaagtaa agcggcatat gcgccggcgg atatccggcg cgccgagctc     3840

gctgatcagc ctcgactgtg cctctagttg ccagccatct gttgtttgcc cctcccccgt     3900

gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat     3960

tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag     4020

caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc     4080

ttctgaggcg gaaagaacca gctggggtta attaaacgcg tttgagtgta tgttaacttc     4140

tgacccactg ggaatgtgat gaaagaaata aaagctgaaa tgaatcattc tctctactat     4200

tattctgata tttcacattc ttaaaataaa gtggtgatcc taactgacct taagacaggg     4260

aatctttact cggattaaat gtcaggaatt gtgaaaaagt gagtttaaat gtatttggct     4320

aaggtgtatg taaacttccg acttcaacta tcgattcgcg cgcgtgcatt ctagttgtgg     4380

tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag     4440

cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc     4500

acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta     4560

actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca     4620

gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc     4680

cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc     4740

tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat     4800

gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt     4860

ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg     4920

aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc     4980

tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt     5040

ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa     5100

gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta     5160

tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa     5220

caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa     5280

ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt     5340

cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt     5400

ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat     5460

cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat     5520

gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc     5580

aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc     5640

acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta     5700

gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga     5760

cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg     5820

cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc     5880

tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat     5940

cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag     6000

gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat     6060

cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa     6120

ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa     6180

gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga     6240

taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg     6300

gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc     6360

acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg     6420

aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact     6480

cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat     6540

atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt     6600

gccacctgac gtcgacggat cgggcctgca g                                    6631


<210>  40
<211>  5812
<212>  DNA
<213>  Homo sapiens

<400>  40
gctagcacca agggcccatc ggtcttcccc ctggcaccct cctccaagag cacctctggg       60

ggcacagcag ccctgggctg cctggtcaag gactacttcc ccgaaccggt gacggtgtcg      120

tggaactcag gcgccctgac cagcggcgtg cacaccttcc cggctgtcct acagtcctca      180

ggactctact ccctcagcag cgtggtgacc gtgccctcca gcagcttggg cacccagacc      240

tacatctgca acgtgaatca caagcccagc aacaccaagg tggacaagaa agttggtgag      300

aggccagcac agggagggag ggtgtctgct ggaagccagg ctcagcgctc ctgcctggac      360

gcatcccggc tatgcagccc cagtccaggg cagcaaggca ggccccgtct gcctcttcac      420

ccggaggcct ctgcccgccc cactcatgct cagggagagg gtcttctggc tttttcccca      480

ggctctgggc aggcacaggc taggtgcccc taacccaggc cctgcacaca aaggggcagg      540

tgctgggctc agacctgcca agagccatat ccgggaggac cctgcccctg acctaagccc      600

accccaaagg ccaaactctc cactccctca gctcggacac cttctctcct cccagattcc      660

agtaactccc aatcttctct ctgcagagcc caaatcttgt gacaaaactc acacatgccc      720

accgtgccca ggtaagccag cccaggcctc gccctccagc tcaaggcggg acaggtgccc      780

tagagtagcc tgcatccagg gacaggcccc agccgggtgc tgacacgtcc acctccatct      840

cttcctcagc acctgaactc ctggggggac cgtcagtctt cctcttcccc ccaaaaccca      900

aggacaccct catgatctcc cggacccctg aggtcacatg cgtggtggtg gacgtgagcc      960

acgaagaccc tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg cataatgcca     1020

agacaaagcc gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc gtcctcaccg     1080

tcctgcacca ggactggctg aatggcaagg agtacaagtg caaggtctcc aacaaagccc     1140

tcccagcccc catcgagaaa accatctcca aagccaaagg tgggacccgt ggggtgcgag     1200

ggccacatgg acagaggccg gctcggccca ccctctgccc tgagagtgac cgctgtacca     1260

acctctgtcc ctacagggca gccccgagaa ccacaggtgt acaccctgcc cccatcccgg     1320

gatgagctga ccaagaacca ggtcagcctg acctgcctgg tcaaaggctt ctatcccagc     1380

gacatcgccg tggagtggga gagcaatggg cagccggaga acaactacaa gaccacgcct     1440

cccgtgctgg actccgacgg ctccttcttc ctctacagca agctcaccgt ggacaagagc     1500

aggtggcagc aggggaacgt cttctcatgc tccgtgatgc atgaggctct gcacaaccac     1560

tacacacaga agagcctctc cctgtctccg ggtaaatgag tgccacggcc ggcaagcccc     1620

cgctccccag gctctcgggg tcgcgcgagg atgcttggca cgtaccccgt gtacatactt     1680

cccaggcacc cagcatggaa ataaagcacc cagcgcttcc ctgggcccct gcgagactgt     1740

gatggttctt tccacgggtc aggccgagtc tgaggcctga gtggcatgag ggaggcagag     1800

tgggtcccac tgtccccaca ctggcccagg ctgtgcaggt gtgcctgggc cgcctagggt     1860

ggggctcagc caggggctgc cctcggcagg gtgggggatt tgccagcgtg gccctccctc     1920

cagcagcagc tgccctgggc tgggccacga gaagccctag gagcccctgg ggacagacac     1980

acagcccctg cctctgtagg agactgtcct gttctgtgag cgccctgtcc tccgacccgc     2040

atgcccactc gggggcatgc ctagtccatg tgcgtaggga caggccctcc ctcacccatc     2100

tacccccacg gcactaaccc ctggcagccc tgcccagcct cgcacccgca tggggacaca     2160

accgactccg gggacatgca ctctcgggcc ctgtggagag actggtccag atgcccacac     2220

acacactcag cccagacccg ttcaacaaac cccgcactga ggttggccgg ccacacggcc     2280

accacacaca cacgtgcacg cctcacacac ggagcctcac ccgggcgaac cgcacagcac     2340

ccagaccaga gcaaggtcct cgcacacgtg aacactcctc ggacacaggc ccccacgagc     2400

cccacgcggc acctcaaggc ccacgagccg ctcggcagct tctccacatg ctgacctgct     2460

cagacaaacc cagccctcct ctcacaaggt gcccctgcag ccgccacaca cacacagggg     2520

atcacacacc acgtcacgtc cctggccctg gcccacttcc cagtgccgcc cttccctgca     2580

gctggggtca catgaggtgt gggcttcacc atcctcctgc cctctgggcc tcagggaggg     2640

acacgggaga cggggagcgg gtcctgctga gggccaggtc gctatctagg gccgggtgtc     2700

tggctgagcc ccggggccaa agctggtgcc cagggcgggc agctgtgggg agctgacctc     2760

aggacattgt tggcccatcc cggccgggcc ctacatcctg ggtcctgcca cagagggaat     2820

cacccccaga ggcccaagcc cagggggaca cagcactgac cacccccttc ctgtccagag     2880

ctgcaactgg aggagagctg tgcggaggcg caggacgggg agctggacgg gctgtggacg     2940

accatcacca tcttcatcac actcttcctg ttaagcgtgt gctacagtgc caccgtcacc     3000

ttcttcaagg tcggccgcac gttgtcccca gctgtccttg acattgtccc ccatgctgtc     3060

acaaactgtc tctgacactg tcccacaggc tgtccccacc tgtccctgac gctgtccccc     3120

atgctctcac aaactgtccc tgacattgtc cccaatgctg cccccacctg tccaacagtg     3180

tcccccaggc tctccccaca tgtccccgac actgtccccc atgctgtccc catctgtccc     3240

caacactgtc ccccaccctg tccccctttg tccccaacac tgtcccccac agtttccacc     3300

tgtccctgac actgtccccc atgctttccc cacctgtccc tgacaccatc ccccactctg     3360

tcccctatag ttcctggccc tgtcccccac gctgtcccct acagtacctg gcactgtccc     3420

ccatgctgtc ccctcctgta tgaaaccctg tcccacatgc tgtccccacc tgtccgtgac     3480

aatatccccc acactgtccc cacctgtccc cgacactctc ctccacgttg ttcttaccta     3540

aacccgacac tttcctccat gctgtcccca cccatctccg acactgtacc ccacgttgtc     3600

cccacctgtc ctcaacactg tcccccatgc tgtccccacc tgtccccaac actctcctcc     3660

atgctgtccc cacctgtccc tgatattgtc ccccatgcag tctccacctg tccccaatgc     3720

tgtcccccag gctgtaccta ccagtacaac actgtccccc atgctgtccc cacctgtccc     3780

tgacactgtc ccccacgctg tcccctcctg tccccgacac tgtcccccac actgtcccca     3840

cctgtcccca acactatcct ccatgctgtc ccctcctgtc cccacctgtc ccctacactg     3900

tcccccatgc tgtccccacc agtccccaaa actttcctcc acactgtccc cacctgtccc     3960

caacactgtc ccccacgcta tcccccctgt ccccgacaat gtccccactg tttcctcctg     4020

ttccctccta tccctgacac tgtccgccat gctgtcccca cctgtccctg acactgtctc     4080

ccactctgtc ccctataatc cctgacactg tcccccacgc cgtcccctcc cgtatgcacc     4140

actgtccccc aagctgtccc cacctgtcct caacacagtc ccccatgctg tccccacctg     4200

tccccaacac tctcctccat gtccccacct gtccctgata ttgtccccca tgcagtcccc     4260

acctgtcccc gatgctgtcc cccgggctgt acctaccagt ccaacactgt cccccacact     4320

ctccccacct gtccctgata ctgtccccca tgctgtcccc acctgtcccg gacactgttc     4380

tccacgctct cccctcctgt ccctgacact gtcccccaca ctgtccccac ctgtccccaa     4440

cactatcctc catcctgtcc caacctgtct cctacactgt cccccatgct gtccccacca     4500

gtccccaaca ctgtcctcca tgctgtcccc catgtcccca acactgtccc ccatgctatc     4560

tcccctgtcc ctgacaatgt ccccactgtt tcctgtcccc tcctatccct gacactgtcc     4620

cccatgctgt ccccacctgt cccccacatg gtctccaccg gtccctgaca ctgtctccca     4680

ctctgtcccc tataatccct gacactgtcc cccacaccgt cccctcctgt atgcaccact     4740

gtcccccatg ctgtccccac ctgtccctga tgctgtcctc cacacagtcc ccacctctcc     4800

ctgacactgt ccccatctct ccccaacact ctcctccatg ctgtccttaa ctgtccccaa     4860

cactcttcca cactctgtct ccacctgtcc ctgacactgt cccccacact gtcctcacct     4920

gtgtctgaca ctgtccccca cgctgtcccc acctgtccct gacgctgtct tctgtgctgt     4980

ccacatgctg ttggtgccct ggctctgctc tctatcacca agcctcagag caggcagtgg     5040

tgaggccatg gcacctgggt ggcatgaggg gccggatggg cctcaggggc agggctgtgg     5100

cctgcgtgga ctgacgggtg ggtgggcctt gggggcagag aggtggcctc agtgccctga     5160

ggggtgggtg gggctcgggg gcagggctgt ggcctcgctc acccctgtgc tgtgccttgc     5220

ctacaggtga agtggatctt ctcctcggtg gtggacctga agcagaccat catccccgac     5280

tacaggaaca tgatcggaca gggggcctag ggccaccctc tgcggggtgt ccagggccgc     5340

ccagacccca cacaccagcc atgggccatg ctcagccacc acccaggcca cacctgcccc     5400

cgacctcacc gccctcaacc ccatgactct ctggcctcgc agttgccctc tgaccctgac     5460

acacctgaca cgcccccctt ccagaccctg tgcatagcag gtctacccca gacctccgct     5520

gcttggtgca tgcagggcac tgggggccag gtgtcccctc agcaggacgt ccttgccctc     5580

cggaccacaa ggtgctcaca caaaaggagg cagtgaccgg tatcccaggc ccccacccag     5640

gcaggacctc gccctggagc caaccccgtc cacgccagcc tcctgaacac aggcgtggtt     5700

tccagatggt gagtgggagc gtcagccgcc aaggtaggga agccacagca ccatcaggcc     5760

ctgttgggga ggcttccgag agctgcgaag gctcactcag acggccttcg aa             5812


<210>  41
<211>  12238
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  transposable Ig-gamma1-HC expression vector in genomic 
       configuration

<400>  41
gaattcttaa ccctagaaag atagtctgcg taaaattgac gcatgcattc ttgaaatatt       60

gctctctctt tctaaatagc gcgaatccgt cgctgtgcat ttaggacatc tcagtcgccg      120

cttggagctc ccgtgaggcg tgcttgtcaa tgcggtaagt gtcactgatt ttgaactata      180

acgaccgcgt gagtcaaaat gacgcatgat tatcttttac gtgactttta agatttaact      240

catacgataa ttatattgtt atttcatgtt ctacttacgt gataacttat tatatatata      300

ttttcttgtt atacctcgag gctagtctag agagtaattc atacaaaagg actcgcccct      360

gccttgggga atcccaggga ccgtcgttaa actcccacta acgtagaacc cagagatcgc      420

tgcgttcccg ccccctcacc cgcccgctct cgtcatcact gaggtggaga agagcatgcg      480

tgaggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg      540

gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa      600

agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt      660

gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt      720

gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga      780

attacttcca cgcccctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag      840

tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga      900

ggcctggctt gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct      960

cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt     1020

tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt     1080

tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg     1140

cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct     1200

ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc     1260

ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa     1320

atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc     1380

ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca     1440

cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta     1500

tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt     1560

gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc     1620

tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgagcgg ccgccatgaa     1680

ttttggactg aggctgattt tcctggtgct gaccctgaaa ggcgtccagt gtcagatcca     1740

gctgcagcag tctggccccg aggtcgtgaa acctggcgcc tccgtgaaga tctcctgcaa     1800

ggcctccggc tacaccttca ccgactacta catcacctgg gtcaagcaga agcccggcca     1860

gggcctggaa tggatcggct ggatctatcc cggctccggc aacaccaagt acaacgagaa     1920

gttcaagggc aaggccaccc tgaccgtgga cacctcctct tccaccgcct tcatgcagct     1980

gtcctccctg acctccgagg ataccgccgt gtacttctgc gccaactacg gcaactattg     2040

gttcgcctac tggggccagg gcacacaagt gaccgtgtct gctgctagca ccaagggccc     2100

atcggtcttc cccctggcac cctcctccaa gagcacctct gggggcacag cagccctggg     2160

ctgcctggtc aaggactact tccccgaacc ggtgacggtg tcgtggaact caggcgccct     2220

gaccagcggc gtgcacacct tcccggctgt cctacagtcc tcaggactct actccctcag     2280

cagcgtggtg accgtgccct ccagcagctt gggcacccag acctacatct gcaacgtgaa     2340

tcacaagccc agcaacacca aggtggacaa gaaagttggt gagaggccag cacagggagg     2400

gagggtgtct gctggaagcc aggctcagcg ctcctgcctg gacgcatccc ggctatgcag     2460

ccccagtcca gggcagcaag gcaggccccg tctgcctctt cacccggagg cctctgcccg     2520

ccccactcat gctcagggag agggtcttct ggctttttcc ccaggctctg ggcaggcaca     2580

ggctaggtgc ccctaaccca ggccctgcac acaaaggggc aggtgctggg ctcagacctg     2640

ccaagagcca tatccgggag gaccctgccc ctgacctaag cccaccccaa aggccaaact     2700

ctccactccc tcagctcgga caccttctct cctcccagat tccagtaact cccaatcttc     2760

tctctgcaga gcccaaatct tgtgacaaaa ctcacacatg cccaccgtgc ccaggtaagc     2820

cagcccaggc ctcgccctcc agctcaaggc gggacaggtg ccctagagta gcctgcatcc     2880

agggacaggc cccagccggg tgctgacacg tccacctcca tctcttcctc agcacctgaa     2940

ctcctggggg gaccgtcagt cttcctcttc cccccaaaac ccaaggacac cctcatgatc     3000

tcccggaccc ctgaggtcac atgcgtggtg gtggacgtga gccacgaaga ccctgaggtc     3060

aagttcaact ggtacgtgga cggcgtggag gtgcataatg ccaagacaaa gccgcgggag     3120

gagcagtaca acagcacgta ccgtgtggtc agcgtcctca ccgtcctgca ccaggactgg     3180

ctgaatggca aggagtacaa gtgcaaggtc tccaacaaag ccctcccagc ccccatcgag     3240

aaaaccatct ccaaagccaa aggtgggacc cgtggggtgc gagggccaca tggacagagg     3300

ccggctcggc ccaccctctg ccctgagagt gaccgctgta ccaacctctg tccctacagg     3360

gcagccccga gaaccacagg tgtacaccct gcccccatcc cgggatgagc tgaccaagaa     3420

ccaggtcagc ctgacctgcc tggtcaaagg cttctatccc agcgacatcg ccgtggagtg     3480

ggagagcaat gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga     3540

cggctccttc ttcctctaca gcaagctcac cgtggacaag agcaggtggc agcaggggaa     3600

cgtcttctca tgctccgtga tgcatgaggc tctgcacaac cactacacac agaagagcct     3660

ctccctgtct ccgggtaaat gagtgccacg gccggcaagc ccccgctccc caggctctcg     3720

gggtcgcgcg aggatgcttg gcacgtaccc cgtgtacata cttcccaggc acccagcatg     3780

gaaataaagc acccagcgct tccctgggcc cctgcgagac tgtgatggtt ctttccacgg     3840

gtcaggccga gtctgaggcc tgagtggcat gagggaggca gagtgggtcc cactgtcccc     3900

acactggccc aggctgtgca ggtgtgcctg ggccgcctag ggtggggctc agccaggggc     3960

tgccctcggc agggtggggg atttgccagc gtggccctcc ctccagcagc agctgccctg     4020

ggctgggcca cgagaagccc taggagcccc tggggacaga cacacagccc ctgcctctgt     4080

aggagactgt cctgttctgt gagcgccctg tcctccgacc cgcatgccca ctcgggggca     4140

tgcctagtcc atgtgcgtag ggacaggccc tccctcaccc atctaccccc acggcactaa     4200

cccctggcag ccctgcccag cctcgcaccc gcatggggac acaaccgact ccggggacat     4260

gcactctcgg gccctgtgga gagactggtc cagatgccca cacacacact cagcccagac     4320

ccgttcaaca aaccccgcac tgaggttggc cggccacacg gccaccacac acacacgtgc     4380

acgcctcaca cacggagcct cacccgggcg aaccgcacag cacccagacc agagcaaggt     4440

cctcgcacac gtgaacactc ctcggacaca ggcccccacg agccccacgc ggcacctcaa     4500

ggcccacgag ccgctcggca gcttctccac atgctgacct gctcagacaa acccagccct     4560

cctctcacaa ggtgcccctg cagccgccac acacacacag gggatcacac accacgtcac     4620

gtccctggcc ctggcccact tcccagtgcc gcccttccct gcagctgggg tcacatgagg     4680

tgtgggcttc accatcctcc tgccctctgg gcctcaggga gggacacggg agacggggag     4740

cgggtcctgc tgagggccag gtcgctatct agggccgggt gtctggctga gccccggggc     4800

caaagctggt gcccagggcg ggcagctgtg gggagctgac ctcaggacat tgttggccca     4860

tcccggccgg gccctacatc ctgggtcctg ccacagaggg aatcaccccc agaggcccaa     4920

gcccaggggg acacagcact gaccaccccc ttcctgtcca gagctgcaac tggaggagag     4980

ctgtgcggag gcgcaggacg gggagctgga cgggctgtgg acgaccatca ccatcttcat     5040

cacactcttc ctgttaagcg tgtgctacag tgccaccgtc accttcttca aggtcggccg     5100

cacgttgtcc ccagctgtcc ttgacattgt cccccatgct gtcacaaact gtctctgaca     5160

ctgtcccaca ggctgtcccc acctgtccct gacgctgtcc cccatgctct cacaaactgt     5220

ccctgacatt gtccccaatg ctgcccccac ctgtccaaca gtgtccccca ggctctcccc     5280

acatgtcccc gacactgtcc cccatgctgt ccccatctgt ccccaacact gtcccccacc     5340

ctgtccccct ttgtccccaa cactgtcccc cacagtttcc acctgtccct gacactgtcc     5400

cccatgcttt ccccacctgt ccctgacacc atcccccact ctgtccccta tagttcctgg     5460

ccctgtcccc cacgctgtcc cctacagtac ctggcactgt cccccatgct gtcccctcct     5520

gtatgaaacc ctgtcccaca tgctgtcccc acctgtccgt gacaatatcc cccacactgt     5580

ccccacctgt ccccgacact ctcctccacg ttgttcttac ctaaacccga cactttcctc     5640

catgctgtcc ccacccatct ccgacactgt accccacgtt gtccccacct gtcctcaaca     5700

ctgtccccca tgctgtcccc acctgtcccc aacactctcc tccatgctgt ccccacctgt     5760

ccctgatatt gtcccccatg cagtctccac ctgtccccaa tgctgtcccc caggctgtac     5820

ctaccagtac aacactgtcc cccatgctgt ccccacctgt ccctgacact gtcccccacg     5880

ctgtcccctc ctgtccccga cactgtcccc cacactgtcc ccacctgtcc ccaacactat     5940

cctccatgct gtcccctcct gtccccacct gtcccctaca ctgtccccca tgctgtcccc     6000

accagtcccc aaaactttcc tccacactgt ccccacctgt ccccaacact gtcccccacg     6060

ctatcccccc tgtccccgac aatgtcccca ctgtttcctc ctgttccctc ctatccctga     6120

cactgtccgc catgctgtcc ccacctgtcc ctgacactgt ctcccactct gtcccctata     6180

atccctgaca ctgtccccca cgccgtcccc tcccgtatgc accactgtcc cccaagctgt     6240

ccccacctgt cctcaacaca gtcccccatg ctgtccccac ctgtccccaa cactctcctc     6300

catgtcccca cctgtccctg atattgtccc ccatgcagtc cccacctgtc cccgatgctg     6360

tcccccgggc tgtacctacc agtccaacac tgtcccccac actctcccca cctgtccctg     6420

atactgtccc ccatgctgtc cccacctgtc ccggacactg ttctccacgc tctcccctcc     6480

tgtccctgac actgtccccc acactgtccc cacctgtccc caacactatc ctccatcctg     6540

tcccaacctg tctcctacac tgtcccccat gctgtcccca ccagtcccca acactgtcct     6600

ccatgctgtc ccccatgtcc ccaacactgt cccccatgct atctcccctg tccctgacaa     6660

tgtccccact gtttcctgtc ccctcctatc cctgacactg tcccccatgc tgtccccacc     6720

tgtcccccac atggtctcca ccggtccctg acactgtctc ccactctgtc ccctataatc     6780

cctgacactg tcccccacac cgtcccctcc tgtatgcacc actgtccccc atgctgtccc     6840

cacctgtccc tgatgctgtc ctccacacag tccccacctc tccctgacac tgtccccatc     6900

tctccccaac actctcctcc atgctgtcct taactgtccc caacactctt ccacactctg     6960

tctccacctg tccctgacac tgtcccccac actgtcctca cctgtgtctg acactgtccc     7020

ccacgctgtc cccacctgtc cctgacgctg tcttctgtgc tgtccacatg ctgttggtgc     7080

cctggctctg ctctctatca ccaagcctca gagcaggcag tggtgaggcc atggcacctg     7140

ggtggcatga ggggccggat gggcctcagg ggcagggctg tggcctgcgt ggactgacgg     7200

gtgggtgggc cttgggggca gagaggtggc ctcagtgccc tgaggggtgg gtggggctcg     7260

ggggcagggc tgtggcctcg ctcacccctg tgctgtgcct tgcctacagg tgaagtggat     7320

cttctcctcg gtggtggacc tgaagcagac catcatcccc gactacagga acatgatcgg     7380

acagggggcc tagggccacc ctctgcgggg tgtccagggc cgcccagacc ccacacacca     7440

gccatgggcc atgctcagcc accacccagg ccacacctgc ccccgacctc accgccctca     7500

accccatgac tctctggcct cgcagttgcc ctctgaccct gacacacctg acacgccccc     7560

cttccagacc ctgtgcatag caggtctacc ccagacctcc gctgcttggt gcatgcaggg     7620

cactgggggc caggtgtccc ctcagcagga cgtccttgcc ctccggacca caaggtgctc     7680

acacaaaagg aggcagtgac cggtatccca ggcccccacc caggcaggac ctcgccctgg     7740

agccaacccc gtccacgcca gcctcctgaa cacaggcgtg gtttccagat ggtgagtggg     7800

agcgtcagcc gccaaggtag ggaagccaca gcaccatcag gccctgttgg ggaggcttcc     7860

gagagctgcg aaggctcact cagacggcct tcgaagggca ggtaagtatc aaggttacaa     7920

gacaggttta aggagaccaa tagaaactgg gcttgtcgag acagagaaga ctcttgcgtt     7980

tctgataggc acctattggt cttactgaca tccactttgc ctttctctcc acaggtgtcc     8040

tacgtagcga tcgccaattc cgcccctctc cctccccccc ccctaacgtt actggccgaa     8100

gccgcttgga ataaggccgg tgtgcgtttg tctatatgtg attttccacc atattgccgt     8160

cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg     8220

gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc     8280

ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc     8340

ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa     8400

aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc     8460

tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg     8520

gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac     8580

gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataagctt     8640

gccacaaccc ggtttaaacg gatcccgcca ccatggtgag caagggcgag gagctgttca     8700

ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg     8760

tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca     8820

ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc     8880

agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc     8940

ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc     9000

gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg     9060

acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca     9120

acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc     9180

acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg     9240

gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca     9300

aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga     9360

tcactctcgg catggacgag ctgtacaagt aaagcggcat atgcgccggc ggatatccgg     9420

cgcgccgagc tcgctgatca gcctcgactg tgcctctagt tgccagccat ctgttgtttg     9480

cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata     9540

aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt     9600

ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt     9660

gggctctatg gcttctgagg cggaaagaac cagctggggt taattaaacg cgtttaaccc     9720

tagaaagata atcatattgt gacgtacgtt aaagataatc atgcgtaaaa ttgacgcatg     9780

tgttttatcg gtctgtatat cgaggtttat ttattaattt gaatagatat taagttttat     9840

tatatttaca cttacatact aataataaat tcaacaaaca atttatttat gtttatttat     9900

ttattaaaaa aaaacaaaaa ctcaaaattt cttctataaa gtaacaaaac ttttatatcg     9960

attcgcgcgc gtgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt    10020

ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt    10080

gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa    10140

agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc    10200

tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag    10260

aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt    10320

cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga    10380

atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg    10440

taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa    10500

aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt    10560

tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct    10620

gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct    10680

cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc    10740

cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt    10800

atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc    10860

tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat    10920

ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa    10980

acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa    11040

aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga    11100

aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct    11160

tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga    11220

cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc    11280

catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg    11340

ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat    11400

aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat    11460

ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg    11520

caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc    11580

attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa    11640

agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc    11700

actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt    11760

ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag    11820

ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt    11880

gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag    11940

atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac    12000

cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc    12060

gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca    12120

gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg    12180

ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gcctgcag      12238


<210>  42
<211>  711
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  murine bcl-2 coding region

<400>  42
atggcgcaag ccgggagaac agggtatgat aaccgggaga tcgtgatgaa gtacatacat       60

tataagctgt cacagagggg ctacgagtgg gatgctggag atgcggacgc ggcgcccctg      120

ggggctgccc ccacccctgg catcttctcc ttccagcctg agagcaaccc aatgcccgct      180

gtgcaccggg acatggctgc caggacgtct cctctcaggc ccctcgttgc caccgctggg      240

cctgcgctca gccctgtgcc acctgtggtc catctgaccc tccgccgggc tggggatgac      300

ttctctcgtc gctaccgtcg tgacttcgca gagatgtcca gtcagctgca cctgacgccc      360

ttcaccgcga ggggacgctt tgccacggtg gtggaggaac tcttcaggga tggggtgaac      420

tgggggagga ttgtggcctt ctttgagttc ggtggggtca tgtgtgtgga gagcgtcaac      480

agggagatgt cacccctggt ggacaacatc gccctgtgga tgactgagta cctgaaccgg      540

catctgcaca cctggatcca ggataacgga ggctgggatg cctttgtgga actatatggc      600

cccagcatgc gacctctgtt tgatttctcc tggctgtctc tgaagaccct gctcagcctg      660

gccctggtcg gggcctgcat cactctgggt gcatacctgg gccacaagtg a               711


<210>  43
<211>  236
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of murine Bcl-2 protein

<400>  43

Met Ala Gln Ala Gly Arg Thr Gly Tyr Asp Asn Arg Glu Ile Val Met 
1               5                   10                  15      


Lys Tyr Ile His Tyr Lys Leu Ser Gln Arg Gly Tyr Glu Trp Asp Ala 
            20                  25                  30          


Gly Asp Ala Asp Ala Ala Pro Leu Gly Ala Ala Pro Thr Pro Gly Ile 
        35                  40                  45              


Phe Ser Phe Gln Pro Glu Ser Asn Pro Met Pro Ala Val His Arg Asp 
    50                  55                  60                  


Met Ala Ala Arg Thr Ser Pro Leu Arg Pro Leu Val Ala Thr Ala Gly 
65                  70                  75                  80  


Pro Ala Leu Ser Pro Val Pro Pro Val Val His Leu Thr Leu Arg Arg 
                85                  90                  95      


Ala Gly Asp Asp Phe Ser Arg Arg Tyr Arg Arg Asp Phe Ala Glu Met 
            100                 105                 110         


Ser Ser Gln Leu His Leu Thr Pro Phe Thr Ala Arg Gly Arg Phe Ala 
        115                 120                 125             


Thr Val Val Glu Glu Leu Phe Arg Asp Gly Val Asn Trp Gly Arg Ile 
    130                 135                 140                 


Val Ala Phe Phe Glu Phe Gly Gly Val Met Cys Val Glu Ser Val Asn 
145                 150                 155                 160 


Arg Glu Met Ser Pro Leu Val Asp Asn Ile Ala Leu Trp Met Thr Glu 
                165                 170                 175     


Tyr Leu Asn Arg His Leu His Thr Trp Ile Gln Asp Asn Gly Gly Trp 
            180                 185                 190         


Asp Ala Phe Val Glu Leu Tyr Gly Pro Ser Met Arg Pro Leu Phe Asp 
        195                 200                 205             


Phe Ser Trp Leu Ser Leu Lys Thr Leu Leu Ser Leu Ala Leu Val Gly 
    210                 215                 220                 


Ala Cys Ile Thr Leu Gly Ala Tyr Leu Gly His Lys 
225                 230                 235     


<210>  44
<211>  6250
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCDNA3.1-hygro(+)-bcl2 mammalian expression vector

<400>  44
gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg       60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg      120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc      180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt      240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata      300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc      360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc      420

attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt      480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt      540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca      600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg      660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc      720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg      780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca      840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc      900

gtttaaactt aagcttggta ccatggcgca agccgggaga acagggtatg ataaccggga      960

gatcgtgatg aagtacatac attataagct gtcacagagg ggctacgagt gggatgctgg     1020

agatgcggac gcggcgcccc tgggggctgc ccccacccct ggcatcttct ccttccagcc     1080

tgagagcaac ccaatgcccg ctgtgcaccg ggacatggct gccaggacgt ctcctctcag     1140

gcccctcgtt gccaccgctg ggcctgcgct cagccctgtg ccacctgtgg tccatctgac     1200

cctccgccgg gctggggatg acttctctcg tcgctaccgt cgtgacttcg cagagatgtc     1260

cagtcagctg cacctgacgc ccttcaccgc gaggggacgc tttgccacgg tggtggagga     1320

actcttcagg gatggggtga actgggggag gattgtggcc ttctttgagt tcggtggggt     1380

catgtgtgtg gagagcgtca acagggagat gtcacccctg gtggacaaca tcgccctgtg     1440

gatgactgag tacctgaacc ggcatctgca cacctggatc caggataacg gaggctggga     1500

tgcctttgtg gaactatatg gccccagcat gcgacctctg tttgatttct cctggctgtc     1560

tctgaagacc ctgctcagcc tggccctggt cggggcctgc atcactctgg gtgcatacct     1620

gggccacaag tgactcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt     1680

gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga     1740

aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag     1800

taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga     1860

agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac     1920

cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg     1980

tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt     2040

cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg     2100

gggcatccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga     2160

ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac     2220

gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc     2280

tatctcggtc tattcttttg atttataagg gattttgggg atttcggcct attggttaaa     2340

aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta     2400

gggtgtggaa agtccccagg ctccccaggc aggcagaagt atgcaaagca tgcatctcaa     2460

ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag     2520

catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct     2580

aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc     2640

agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg     2700

aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca     2760

gcacgtgatg aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa     2820

gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag     2880

cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta     2940

caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct     3000

tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt     3060

cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc     3120

catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc     3180

gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca     3240

tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct     3300

cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga     3360

tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag     3420

cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg     3480

gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg     3540

atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt     3600

ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg     3660

atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac     3720

cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag     3780

ggcaaaggaa tagcacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt     3840

gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat     3900

gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag     3960

caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt     4020

gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt     4080

ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca     4140

caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact     4200

cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct     4260

gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc     4320

ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca     4380

ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg     4440

agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca     4500

taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa     4560

cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc     4620

tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc     4680

gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct     4740

gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg     4800

tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag     4860

gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta     4920

cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg     4980

aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt     5040

tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt     5100

ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag     5160

attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat     5220

ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc     5280

tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat     5340

aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc     5400

acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag     5460

aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag     5520

agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt     5580

ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg     5640

agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt     5700

tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc     5760

tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc     5820

attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa     5880

taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg     5940

aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc     6000

caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag     6060

gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt     6120

cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt     6180

tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc     6240

acctgacgtc                                                            6250


<210>  45
<211>  3064
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pUC57_VkappaK1-5

<400>  45
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa      420

tgcatctaga tccaatccaa tgcggccgca tggacatgag ggtccccgct cagctcctgg      480

ggctcctgct gctctggctc ccaggtgcca aatgtgacat ccagatgacc cagtctcctt      540

ccaccctgtc tgcatctgta ggagacagag tcaccatcac ttgccgggcc agtcagagta      600

ttagtagctg gttggcctgg tatcagcaga aaccagggaa agcccctaag ctcctgatct      660

atgatgcctc cagtttggaa agtggggtcc catcaaggtt cagcggcagt ggatctggga      720

cagaattcac tctcaccatc agcagcctgc agcctgatga ttttgcaact tattactgcc      780

aacagatcgg atcccgggcc cgtcgactgc agaggcctgc atgcaagctt ggcgtaatca      840

tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga      900

gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt      960

gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga     1020

atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc     1080

actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg     1140

gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc     1200

cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc     1260

ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga     1320

ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc     1380

ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat     1440

agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg     1500

cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc     1560

aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga     1620

gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact     1680

agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt     1740

ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag     1800

cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg     1860

tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa     1920

aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata     1980

tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg     2040

atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata     2100

cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg     2160

gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct     2220

gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt     2280

tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc     2340

tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga     2400

tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt     2460

aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc     2520

atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa     2580

tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca     2640

catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca     2700

aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct     2760

tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc     2820

gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa     2880

tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt     2940

tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc     3000

taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt     3060

cgtc                                                                  3064


<210>  46
<211>  3100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pUC57_JkappaK2-C-kappaK

<400>  46
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa      420

tgcatctaga tccaatccaa tagtactttt ggccagggga ccaagctgga gatcaaacgt      480

acggtggctg caccatctgt cttcatcttc ccgccatctg atgagcagtt gaaatctgga      540

actgcctctg ttgtgtgcct gctgaataac ttctatccca gagaggccaa agtacagtgg      600

aaggtggata acgccctcca atcgggtaac tcccaggaga gtgtcacaga gcaggacagc      660

aaggacagca cctacagcct cagcagcacc ctgacgctga gcaaagcaga ctacgagaaa      720

cacaaagtct acgcctgcga agtcacccat cagggcctga gctcgcccgt cacaaagagc      780

ttcaacaggg gagagtgtta gcgctttcga aattggattg gatcggatcc cgggcccgtc      840

gactgcagag gcctgcatgc aagcttggcg taatcatggt catagctgtt tcctgtgtga      900

aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc      960

tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc     1020

cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc     1080

ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt     1140

cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca     1200

ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa     1260

aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat     1320

cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc     1380

cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc     1440

gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt     1500

tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac     1560

cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg     1620

ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca     1680

gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc     1740

gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa     1800

accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa     1860

ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac     1920

tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta     1980

aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt     2040

taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata     2100

gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc     2160

agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac     2220

cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag     2280

tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac     2340

gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc     2400

agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg     2460

gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc     2520

atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct     2580

gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc     2640

tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc     2700

atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc     2760

agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc     2820

gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca     2880

cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt     2940

tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt     3000

ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca     3060

ttaacctata aaaataggcg tatcacgagg ccctttcgtc                           3100


<210>  47
<211>  411
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VkappaK1-5 PCR product

<400>  47
cgttgtaaaa cgacggccag tgaattcgag ctcggtacct cgcgaatgca tctagatcca       60

atccaatgcg gccgcatgga catgagggtc cccgctcagc tcctggggct cctgctgctc      120

tggctcccag gtgccaaatg tgacatccag atgacccagt ctccttccac cctgtctgca      180

tctgtaggag acagagtcac catcacttgc cgggccagtc agagtattag tagctggttg      240

gcctggtatc agcagaaacc agggaaagcc cctaagctcc tgatctatga tgcctccagt      300

ttggaaagtg gggtcccatc aaggttcagc ggcagtggat ctgggacaga attcactctc      360

accatcagca gcctgcagcc tgatgatttt gcaacttatt actgccaaca g               411


<210>  48
<211>  495
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NNK6-JkappaK2-CK-kappa PCR product


<220>
<221>  misc_feature
<222>  (22)..(23)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (25)..(26)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (28)..(29)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (31)..(32)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (37)..(38)
<223>  n is a, c, g, or t

<400>  48
gcaacttatt actgccaaca gnnknnknnk nnknnknnka cttttggcca ggggaccaag       60

ctggagatca aacgtacggt ggctgcacca tctgtcttca tcttcccgcc atctgatgag      120

cagttgaaat ctggaactgc ctctgttgtg tgcctgctga ataacttcta tcccagagag      180

gccaaagtac agtggaaggt ggataacgcc ctccaatcgg gtaactccca ggagagtgtc      240

acagagcagg acagcaagga cagcacctac agcctcagca gcaccctgac gctgagcaaa      300

gcagactacg agaaacacaa agtctacgcc tgcgaagtca cccatcaggg cctgagctcg      360

cccgtcacaa agagcttcaa caggggagag tgttagcgct ttcgaaattg gattggatcg      420

gatcccgggc ccgtcgactg cagaggcctg catgcaagct tggcgtaatc atggtcatag      480

ctgtttcctg tgtga                                                       495


<210>  49
<211>  3083
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pUC57_VH3-30

<400>  49
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa      420

tgcatctaga tccaatccaa tcgcacagta atacacagcc gtgtcctcag ctctcaggct      480

gttcatttgc agatacagcg tgttcttgga attgtctctg gagatggtga atcggccctt      540

cacggagtct gcgtagtatt tattacttcc atcatatgat ataactgcca cccactctag      600

ccccttgcct ggagcctggc ggacccagtg catagcatag ctactgaagg tgaatccaga      660

ggctgcacag gagagtctca gggacctccc aggctggacc acgcctcccc cagactccac      720

cagctgcacc tgacactgga cacctcttaa aagagcaacg aggaaaaccc agctcagccc      780

aaactccatg cggccgcatt ggatatcgga tcccgggccc gtcgactgca gaggcctgca      840

tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac      900

aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt      960

gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc     1020

gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg     1080

ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt     1140

atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa     1200

gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc     1260

gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag     1320

gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt     1380

gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg     1440

aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg     1500

ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg     1560

taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac     1620

tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg     1680

gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt     1740

taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg     1800

tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc     1860

tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt     1920

ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt     1980

taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag     2040

tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt     2100

cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc     2160

gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc     2220

cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg     2280

ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac     2340

aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg     2400

atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc     2460

tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact     2520

gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc     2580

aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat     2640

acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc     2700

ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac     2760

tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa     2820

aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact     2880

catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg     2940

atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg     3000

aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag     3060

gcgtatcacg aggccctttc gtc                                             3083


<210>  50
<211>  2760
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pUC57_JH4

<400>  50
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa      420

tgcatctaga tgacgacatt ggggtcaggg aaccctggtc accgtctcct cagctagcga      480

catcggatcc cgggcccgtc gactgcagag gcctgcatgc aagcttggcg taatcatggt      540

catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg      600

gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt      660

tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg      720

gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg      780

actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa      840

tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc      900

aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc      960

ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat     1020

aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc     1080

cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct     1140

cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg     1200

aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc     1260

cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga     1320

ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa     1380

gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta     1440

gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc     1500

agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg     1560

acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga     1620

tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg     1680

agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct     1740

gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg     1800

agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc     1860

cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa     1920

ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc     1980

cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt     2040

cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc     2100

ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt     2160

tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc     2220

catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt     2280

gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata     2340

gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga     2400

tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag     2460

catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa     2520

aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt     2580

attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga     2640

aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag     2700

aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc     2760


<210>  51
<211>  366
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VH3-30 PCR product

<400>  51
gatatccaat gcggccgcat ggagtttggg ctgagctggg ttttcctcgt tgctctttta       60

agaggtgtcc agtgtcaggt gcagctggtg gagtctgggg gaggcgtggt ccagcctggg      120

aggtccctga gactctcctg tgcagcctct ggattcacct tcagtagcta tgctatgcac      180

tgggtccgcc aggctccagg caaggggcta gagtgggtgg cagttatatc atatgatgga      240

agtaataaat actacgcaga ctccgtgaag ggccgattca ccatctccag agacaattcc      300

aagaacacgc tgtatctgca aatgaacagc ctgagagctg aggacacggc tgtgtattac      360

tgtgcg                                                                 366


<210>  52
<211>  299
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NNK4-JH4 PCR product


<220>
<221>  misc_feature
<222>  (26)..(27)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (32)..(33)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (35)..(36)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (41)..(42)
<223>  n is a, c, g, or t

<400>  52
cacggctgtg tattactgtg cgargnnknn knnknnkgac nnktggggcc aaggaaccct       60

ggtcaccgtc tcctcagcta gcgacatcgg atcccgggcc cgtcgactgc agaggcctgc      120

atgcaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca      180

caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag      240

tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctg       299


<210>  53
<211>  305
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NNK6-JH4 PCR product


<220>
<221>  misc_feature
<222>  (26)..(27)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (32)..(33)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (35)..(36)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (38)..(39)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (41)..(42)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (47)..(48)
<223>  n is a, c, g, or t

<400>  53
cacggctgtg tattactgtg cgargnnknn knnknnknnk nnkgacnnkt ggggccaagg       60

aaccctggtc accgtctcct cagctagcga catcggatcc cgggcccgtc gactgcagag      120

gcctgcatgc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc      180

cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct      240

aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa      300

acctg                                                                  305


<210>  54
<211>  311
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NNK8-JH4 PCR product


<220>
<221>  misc_feature
<222>  (26)..(27)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (32)..(33)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (35)..(36)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (38)..(39)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (41)..(42)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (44)..(45)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (47)..(48)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (53)..(54)
<223>  n is a, c, g, or t

<400>  54
cacggctgtg tattactgtg cgargnnknn knnknnknnk nnknnknnkg acnnktgggg       60

ccaaggaacc ctggtcaccg tctcctcagc tagcgacatc ggatcccggg cccgtcgact      120

gcagaggcct gcatgcaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt      180

gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg      240

gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt      300

cgggaaacct g                                                           311


<210>  55
<211>  317
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NNK10-JH4 PCR product


<220>
<221>  misc_feature
<222>  (26)..(27)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (32)..(33)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (35)..(36)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (38)..(39)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (41)..(42)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (44)..(45)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (47)..(48)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (50)..(51)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (53)..(54)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (59)..(60)
<223>  n is a, c, g, or t

<400>  55
cacggctgtg tattactgtg cgargnnknn knnknnknnk nnknnknnkn nknnkgacnn       60

ktggggccaa ggaaccctgg tcaccgtctc ctcagctagc gacatcggat cccgggcccg      120

tcgactgcag aggcctgcat gcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt      180

gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag      240

cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt      300

tccagtcggg aaacctg                                                     317


