                         SEQUENCE LISTING

<110>  Ma, Buyong
       Nellis, David
       Zhu, Jianwei
       Wood, David
 
<120>  REVERSIBLE REGULATION OF INTEIN ACTIVITY THROUGH ENGINEERED NEW 
       ZINC BINDING DOMAIN

<130>  26227.0038P1

<150>  61/678,303
<151>  2012-08-01

<150>  61/777,068
<151>  2013-03-12

<160>  44    

<170>  PatentIn version 3.5

<210>  1
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; reversible zinc binding motif

<400>  1

Gly Glu Gly His 
1               


<210>  2
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; reversible zinc binding motif

<400>  2

Gly Asp Gly His 
1               


<210>  3
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; reversible zinc binding motif

<400>  3

Gly Glu Gly His His 
1               5   


<210>  4
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; reversible zinc binding motif

<400>  4

Gly Glu Gly His Gly 
1               5   


<210>  5
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; reversible zinc binding motif

<400>  5

Gly Asp Gly His His 
1               5   


<210>  6
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; reversible zinc binding motif

<400>  6

Gly Asp Gly His Gly 
1               5   


<210>  7
<211>  168
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; intein peptide sequence

<400>  7

Ala Leu Ala Glu Gly Thr Arg Ile Phe Asp Pro Val Thr Gly Thr Thr 
1               5                   10                  15      


His Arg Ile Glu Asp Val Val Gly Gly Arg Lys Pro Ile His Val Val 
            20                  25                  30          


Ala Ala Ala Lys Asp Gly Thr Leu His Ala Arg Pro Val Val Ser Trp 
        35                  40                  45              


Phe Asp Gln Gly Thr Arg Asp Val Ile Gly Leu Arg Ile Ala Gly Gly 
    50                  55                  60                  


Ala Ile Leu Trp Ala Thr Pro Asp His Lys Val Leu Thr Glu Tyr Gly 
65                  70                  75                  80  


Trp Arg Ala Ala Gly Glu Leu Arg Lys Gly Asp Arg Val Ala Gln Pro 
                85                  90                  95      


Arg Arg Phe Asp Gly Phe Gly Asp Ser Ala Pro Ile Pro Ala Arg Val 
            100                 105                 110         


Gln Ala Leu Ala Asp Ala Leu Asp Asp Lys Phe Leu His Asp Met Leu 
        115                 120                 125             


Ala Glu Glu Leu Arg Tyr Ser Val Ile Arg Glu Val Leu Pro Thr Arg 
    130                 135                 140                 


Arg Ala Arg Thr Phe Gly Leu Glu Val Glu Glu Leu His Thr Leu Val 
145                 150                 155                 160 


Ala Glu Gly Val Val Val His Asn 
                165             


<210>  8
<211>  504
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; intein sequence

<400>  8
gccctcgcag agggcactcg gatcttcgat ccggtcaccg gtacaacgca tcgcatcgag       60

gatgttgtcg gtgggcgcaa gcctattcat gtcgtggctg ctgccaagga cggaacgctg      120

catgcgcggc ccgtggtgtc ctggttcgac cagggaacgc gggatgtgat cgggttgcgg      180

atcgccggtg gcgccatcct gtgggcgaca cccgatcaca aggtgctgac agagtacggc      240

tggcgtgccg ccggggaact ccgcaaggga gacagggtgg cgcaaccgcg acgcttcgat      300

ggattcggtg acagtgcgcc gattccggcg cgcgtgcagg cgctcgcgga tgccctggat      360

gacaaattcc tgcacgacat gctggcggaa gaactccgct attccgtgat ccgagaagtg      420

ctgccaacgc ggcgggcacg aacgttcggc ctcgaggtcg aggaactgca caccctcgtc      480

gccgaagggg ttgttgtaca caac                                             504


<210>  9
<211>  168
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; intein peptide sequence

<400>  9

His Leu Ala Glu Gly Thr Arg Ile Phe Asp Pro Val Thr Gly Thr Thr 
1               5                   10                  15      


His Arg Ile Glu Asp Val Val Gly Gly Arg Lys Pro Ile His Val Val 
            20                  25                  30          


Ala Ala Ala Lys Asp Gly Thr Leu His Ala Arg Pro Val Val Ser Trp 
        35                  40                  45              


Phe Asp Gln Gly Thr Arg Asp Val Ile Gly Leu Arg Ile Ala Gly Gly 
    50                  55                  60                  


Ala Ile Leu Trp Ala Thr Pro Asp His Lys Val Leu Thr Glu Tyr Gly 
65                  70                  75                  80  


Trp Arg Ala Ala Gly Glu Leu Arg Lys Gly Asp Arg Val Ala Gln Pro 
                85                  90                  95      


Arg Arg Phe Asp Gly Phe Gly Asp Ser Ala Pro Ile Pro Ala Arg Val 
            100                 105                 110         


Gln Ala Leu Ala Asp Ala Leu Asp Asp Lys Phe Leu His Asp Met Leu 
        115                 120                 125             


Ala Glu Glu Leu Arg Tyr Ser Val Ile Arg Glu Val Leu Pro Thr Arg 
    130                 135                 140                 


Arg Ala Arg Thr Phe Gly Leu Glu Val Glu Glu Leu His Thr Leu Val 
145                 150                 155                 160 


Ala Glu Gly Val Val Val His Asn 
                165             


<210>  10
<211>  504
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; intein sequence

<400>  10
cacctcgcag agggcactcg gatcttcgat ccggtcaccg gtacaacgca tcgcatcgag       60

gatgttgtcg gtgggcgcaa gcctattcat gtcgtggctg ctgccaagga cggaacgctg      120

catgcgcggc ccgtggtgtc ctggttcgac cagggaacgc gggatgtgat cgggttgcgg      180

atcgccggtg gcgccatcct gtgggcgaca cccgatcaca aggtgctgac agagtacggc      240

tggcgtgccg ccggggaact ccgcaaggga gacagggtgg cgcaaccgcg acgcttcgat      300

ggattcggtg acagtgcgcc gattccggcg cgcgtgcagg cgctcgcgga tgccctggat      360

gacaaattcc tgcacgacat gctggcggaa gaactccgct attccgtgat ccgagaagtg      420

ctgccaacgc ggcgggcacg aacgttcggc ctcgaggtcg aggaactgca caccctcgtc      480

gccgaagggg ttgttgtaca caac                                             504


<210>  11
<211>  172
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; zinc binding motif plus intein peptide

<400>  11

Gly Glu Gly His His Leu Ala Glu Gly Thr Arg Ile Phe Asp Pro Val 
1               5                   10                  15      


Thr Gly Thr Thr His Arg Ile Glu Asp Val Val Gly Gly Arg Lys Pro 
            20                  25                  30          


Ile His Val Val Ala Ala Ala Lys Asp Gly Thr Leu His Ala Arg Pro 
        35                  40                  45              


Val Val Ser Trp Phe Asp Gln Gly Thr Arg Asp Val Ile Gly Leu Arg 
    50                  55                  60                  


Ile Ala Gly Gly Ala Ile Leu Trp Ala Thr Pro Asp His Lys Val Leu 
65                  70                  75                  80  


Thr Glu Tyr Gly Trp Arg Ala Ala Gly Glu Leu Arg Lys Gly Asp Arg 
                85                  90                  95      


Val Ala Gln Pro Arg Arg Phe Asp Gly Phe Gly Asp Ser Ala Pro Ile 
            100                 105                 110         


Pro Ala Arg Val Gln Ala Leu Ala Asp Ala Leu Asp Asp Lys Phe Leu 
        115                 120                 125             


His Asp Met Leu Ala Glu Glu Leu Arg Tyr Ser Val Ile Arg Glu Val 
    130                 135                 140                 


Leu Pro Thr Arg Arg Ala Arg Thr Phe Gly Leu Glu Val Glu Glu Leu 
145                 150                 155                 160 


His Thr Leu Val Ala Glu Gly Val Val Val His Asn 
                165                 170         


<210>  12
<211>  516
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; zinc binding motif plus intein sequence

<400>  12
ggagagggac atcacctcgc agagggcact cggatcttcg atccggtcac cggtacaacg       60

catcgcatcg aggatgttgt cggtgggcgc aagcctattc atgtcgtggc tgctgccaag      120

gacggaacgc tgcatgcgcg gcccgtggtg tcctggttcg accagggaac gcgggatgtg      180

atcgggttgc ggatcgccgg tggcgccatc ctgtgggcga cacccgatca caaggtgctg      240

acagagtacg gctggcgtgc cgccggggaa ctccgcaagg gagacagggt ggcgcaaccg      300

cgacgcttcg atggattcgg tgacagtgcg ccgattccgg cgcgcgtgca ggcgctcgcg      360

gatgccctgg atgacaaatt cctgcacgac atgctggcgg aagaactccg ctattccgtg      420

atccgagaag tgctgccaac gcggcgggca cgaacgttcg gcctcgaggt cgaggaactg      480

cacaccctcg tcgccgaagg ggttgttgta cacaac                                516


<210>  13
<211>  168
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; intein peptide sequence

<400>  13

Cys Leu Ala Glu Gly Thr Arg Ile Phe Asp Pro Val Thr Gly Thr Thr 
1               5                   10                  15      


His Arg Ile Glu Asp Val Val Gly Gly Arg Lys Pro Ile His Val Val 
            20                  25                  30          


Ala Ala Ala Lys Asp Gly Thr Leu His Ala Arg Pro Val Val Ser Trp 
        35                  40                  45              


Phe Asp Gln Gly Thr Arg Asp Val Ile Gly Leu Arg Ile Ala Gly Gly 
    50                  55                  60                  


Ala Ile Leu Trp Ala Thr Pro Asp His Lys Val Leu Thr Glu Tyr Gly 
65                  70                  75                  80  


Trp Arg Ala Ala Gly Glu Leu Arg Lys Gly Asp Arg Val Ala Gln Pro 
                85                  90                  95      


Arg Arg Phe Asp Gly Phe Gly Asp Ser Ala Pro Ile Pro Ala Arg Val 
            100                 105                 110         


Gln Ala Leu Ala Asp Ala Leu Asp Asp Lys Phe Leu His Asp Met Leu 
        115                 120                 125             


Ala Glu Glu Leu Arg Tyr Ser Val Ile Arg Glu Val Leu Pro Thr Arg 
    130                 135                 140                 


Arg Ala Arg Thr Phe Gly Leu Glu Val Glu Glu Leu His Thr Leu Val 
145                 150                 155                 160 


Ala Glu Gly Val Val Val His Asn 
                165             


<210>  14
<211>  504
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; intein sequence

<400>  14
tgcctcgcag agggcactcg gatcttcgat ccggtcaccg gtacaacgca tcgcatcgag       60

gatgttgtcg gtgggcgcaa gcctattcat gtcgtggctg ctgccaagga cggaacgctg      120

catgcgcggc ccgtggtgtc ctggttcgac cagggaacgc gggatgtgat cgggttgcgg      180

atcgccggtg gcgccatcct gtgggcgaca cccgatcaca aggtgctgac agagtacggc      240

tggcgtgccg ccggggaact ccgcaaggga gacagggtgg cgcaaccgcg acgcttcgat      300

ggattcggtg acagtgcgcc gattccggcg cgcgtgcagg cgctcgcgga tgccctggat      360

gacaaattcc tgcacgacat gctggcggaa gaactccgct attccgtgat ccgagaagtg      420

ctgccaacgc ggcgggcacg aacgttcggc ctcgaggtcg aggaactgca caccctcgtc      480

gccgaagggg ttgttgtaca caac                                             504


<210>  15
<211>  172
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; intein peptide plus zinc binding motif

<400>  15

Gly Glu Gly His Cys Leu Ala Glu Gly Thr Arg Ile Phe Asp Pro Val 
1               5                   10                  15      


Thr Gly Thr Thr His Arg Ile Glu Asp Val Val Gly Gly Arg Lys Pro 
            20                  25                  30          


Ile His Val Val Ala Ala Ala Lys Asp Gly Thr Leu His Ala Arg Pro 
        35                  40                  45              


Val Val Ser Trp Phe Asp Gln Gly Thr Arg Asp Val Ile Gly Leu Arg 
    50                  55                  60                  


Ile Ala Gly Gly Ala Ile Leu Trp Ala Thr Pro Asp His Lys Val Leu 
65                  70                  75                  80  


Thr Glu Tyr Gly Trp Arg Ala Ala Gly Glu Leu Arg Lys Gly Asp Arg 
                85                  90                  95      


Val Ala Gln Pro Arg Arg Phe Asp Gly Phe Gly Asp Ser Ala Pro Ile 
            100                 105                 110         


Pro Ala Arg Val Gln Ala Leu Ala Asp Ala Leu Asp Asp Lys Phe Leu 
        115                 120                 125             


His Asp Met Leu Ala Glu Glu Leu Arg Tyr Ser Val Ile Arg Glu Val 
    130                 135                 140                 


Leu Pro Thr Arg Arg Ala Arg Thr Phe Gly Leu Glu Val Glu Glu Leu 
145                 150                 155                 160 


His Thr Leu Val Ala Glu Gly Val Val Val His Asn 
                165                 170         


<210>  16
<211>  516
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; intein plus zinc binding motif sequence

<400>  16
ggagagggac attgcctcgc agagggcact cggatcttcg atccggtcac cggtacaacg       60

catcgcatcg aggatgttgt cggtgggcgc aagcctattc atgtcgtggc tgctgccaag      120

gacggaacgc tgcatgcgcg gcccgtggtg tcctggttcg accagggaac gcgggatgtg      180

atcgggttgc ggatcgccgg tggcgccatc ctgtgggcga cacccgatca caaggtgctg      240

acagagtacg gctggcgtgc cgccggggaa ctccgcaagg gagacagggt ggcgcaaccg      300

cgacgcttcg atggattcgg tgacagtgcg ccgattccgg cgcgcgtgca ggcgctcgcg      360

gatgccctgg atgacaaatt cctgcacgac atgctggcgg aagaactccg ctattccgtg      420

atccgagaag tgctgccaac gcggcgggca cgaacgttcg gcctcgaggt cgaggaactg      480

cacaccctcg tcgccgaagg ggttgttgta cacaac                                516


<210>  17
<211>  168
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; intein peptide sequence

<400>  17

Ala Leu Ala Glu Gly Thr Arg Ile Phe Asp Pro Val Thr Gly Thr Thr 
1               5                   10                  15      


His Arg Ile Glu Asp Val Val Gly Gly Arg Lys Pro Ile His Val Val 
            20                  25                  30          


Ala Ala Ala Lys Asp Gly Thr Leu His Ala Arg Pro Val Val Ser Trp 
        35                  40                  45              


Phe Asp Gln Gly Thr Arg Asp Val Ile Gly Leu Arg Ile Ala Gly Gly 
    50                  55                  60                  


Ala Ile Leu Trp Ala Thr Pro Asp His Lys Val Leu Thr Glu Tyr Gly 
65                  70                  75                  80  


Trp Arg Ala Ala Gly Glu Leu Arg Lys Gly Asp Arg Val Ala Gln Pro 
                85                  90                  95      


Arg Arg Phe Asp Gly Phe Gly Asp Ser Ala Pro Ile Pro Ala Arg Val 
            100                 105                 110         


Gln Ala Leu Ala Asp Ala Leu Asp Asp Lys Phe Leu His Asp Met Leu 
        115                 120                 125             


Ala Glu Glu Leu Arg Tyr Ser Val Ile Arg Glu Val Leu Pro Thr Arg 
    130                 135                 140                 


Arg Ala Arg Thr Phe Gly Leu Glu Val Glu Glu Leu His Thr Leu Val 
145                 150                 155                 160 


Ala Glu Gly Val Val Val His Asn 
                165             


<210>  18
<211>  504
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; intein sequence

<400>  18
gccctcgcag agggcactcg gatcttcgat ccggtcaccg gtacaacgca tcgcatcgag       60

gatgttgtcg gtgggcgcaa gcctattcat gtcgtggctg ctgccaagga cggaacgctg      120

catgcgcggc ccgtggtgtc ctggttcgac cagggaacgc gggatgtgat cgggttgcgg      180

atcgccggtg gcgccatcct gtgggcgaca cccgatcaca aggtgctgac agagtacggc      240

tggcgtgccg ccggggaact ccgcaaggga gacagggtgg cgcaaccgcg acgcttcgat      300

ggattcggtg acagtgcgcc gattccggcg cgcgtgcagg cgctcgcgga tgccctggat      360

gacaaattcc tgcacgacat gctggcggaa gaactccgct attccgtgat ccgagaagtg      420

ctgccaacgc ggcgggcacg aacgttcggc ctcgaggtcg aggaactgca caccctcgtc      480

gccgaagggg ttgttgtaca caac                                             504


<210>  19
<211>  173
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; intein peptide plus zinc binding motif

<400>  19

Gly Glu Gly His His Ala Leu Ala Glu Gly Thr Arg Ile Phe Asp Pro 
1               5                   10                  15      


Val Thr Gly Thr Thr His Arg Ile Glu Asp Val Val Gly Gly Arg Lys 
            20                  25                  30          


Pro Ile His Val Val Ala Ala Ala Lys Asp Gly Thr Leu His Ala Arg 
        35                  40                  45              


Pro Val Val Ser Trp Phe Asp Gln Gly Thr Arg Asp Val Ile Gly Leu 
    50                  55                  60                  


Arg Ile Ala Gly Gly Ala Ile Leu Trp Ala Thr Pro Asp His Lys Val 
65                  70                  75                  80  


Leu Thr Glu Tyr Gly Trp Arg Ala Ala Gly Glu Leu Arg Lys Gly Asp 
                85                  90                  95      


Arg Val Ala Gln Pro Arg Arg Phe Asp Gly Phe Gly Asp Ser Ala Pro 
            100                 105                 110         


Ile Pro Ala Arg Val Gln Ala Leu Ala Asp Ala Leu Asp Asp Lys Phe 
        115                 120                 125             


Leu His Asp Met Leu Ala Glu Glu Leu Arg Tyr Ser Val Ile Arg Glu 
    130                 135                 140                 


Val Leu Pro Thr Arg Arg Ala Arg Thr Phe Gly Leu Glu Val Glu Glu 
145                 150                 155                 160 


Leu His Thr Leu Val Ala Glu Gly Val Val Val His Asn 
                165                 170             


<210>  20
<211>  519
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; intein plus zinc binding motif sequence

<400>  20
ggagagggac atcatgccct cgcagagggc actcggatct tcgatccggt caccggtaca       60

acgcatcgca tcgaggatgt tgtcggtggg cgcaagccta ttcatgtcgt ggctgctgcc      120

aaggacggaa cgctgcatgc gcggcccgtg gtgtcctggt tcgaccaggg aacgcgggat      180

gtgatcgggt tgcggatcgc cggtggcgcc atcctgtggg cgacacccga tcacaaggtg      240

ctgacagagt acggctggcg tgccgccggg gaactccgca agggagacag ggtggcgcaa      300

ccgcgacgct tcgatggatt cggtgacagt gcgccgattc cggcgcgcgt gcaggcgctc      360

gcggatgccc tggatgacaa attcctgcac gacatgctgg cggaagaact ccgctattcc      420

gtgatccgag aagtgctgcc aacgcggcgg gcacgaacgt tcggcctcga ggtcgaggaa      480

ctgcacaccc tcgtcgccga aggggttgtt gtacacaac                             519


<210>  21
<211>  168
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; intein peptide

<400>  21

Ala Leu Ala Glu Gly Thr Arg Ile Phe Asp Pro Val Thr Gly Thr Thr 
1               5                   10                  15      


His Arg Ile Glu Asp Val Val Gly Gly Arg Lys Pro Ile His Val Val 
            20                  25                  30          


Ala Ala Ala Lys Asp Gly Thr Leu His Ala Arg Pro Val Val Ser Trp 
        35                  40                  45              


Phe Asp Gln Gly Thr Arg Asp Val Ile Gly Leu Arg Ile Ala Gly Gly 
    50                  55                  60                  


Ala Ile Leu Trp Ala Thr Pro Asp His Lys Val Leu Thr Glu Tyr Gly 
65                  70                  75                  80  


Trp Arg Ala Ala Gly Glu Leu Arg Lys Gly Asp Arg Val Ala Gln Pro 
                85                  90                  95      


Arg Arg Phe Asp Gly Phe Gly Asp Ser Ala Pro Ile Pro Ala Arg Val 
            100                 105                 110         


Gln Ala Leu Ala Asp Ala Leu Asp Asp Lys Phe Leu His Asp Met Leu 
        115                 120                 125             


Ala Glu Glu Leu Arg Tyr Ser Val Ile Arg Glu Val Leu Pro Thr Arg 
    130                 135                 140                 


Arg Ala Arg Thr Phe Gly Leu Glu Val Glu Glu Leu His Thr Leu Val 
145                 150                 155                 160 


Ala Glu Gly Val Val Val His Asn 
                165             


<210>  22
<211>  504
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; intein sequence

<400>  22
gccctcgcag agggcactcg gatcttcgat ccggtcaccg gtacaacgca tcgcatcgag       60

gatgttgtcg gtgggcgcaa gcctattcat gtcgtggctg ctgccaagga cggaacgctg      120

catgcgcggc ccgtggtgtc ctggttcgac cagggaacgc gggatgtgat cgggttgcgg      180

atcgccggtg gcgccatcct gtgggcgaca cccgatcaca aggtgctgac agagtacggc      240

tggcgtgccg ccggggaact ccgcaaggga gacagggtgg cgcaaccgcg acgcttcgat      300

ggattcggtg acagtgcgcc gattccggcg cgcgtgcagg cgctcgcgga tgccctggat      360

gacaaattcc tgcacgacat gctggcggaa gaactccgct attccgtgat ccgagaagtg      420

ctgccaacgc ggcgggcacg aacgttcggc ctcgaggtcg aggaactgca caccctcgtc      480

gccgaagggg ttgttgtaca caac                                             504


<210>  23
<211>  173
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; intein peptide plus zinc binding motif

<400>  23

Gly Asp Gly His His Ala Leu Ala Glu Gly Thr Arg Ile Phe Asp Pro 
1               5                   10                  15      


Val Thr Gly Thr Thr His Arg Ile Glu Asp Val Val Gly Gly Arg Lys 
            20                  25                  30          


Pro Ile His Val Val Ala Ala Ala Lys Asp Gly Thr Leu His Ala Arg 
        35                  40                  45              


Pro Val Val Ser Trp Phe Asp Gln Gly Thr Arg Asp Val Ile Gly Leu 
    50                  55                  60                  


Arg Ile Ala Gly Gly Ala Ile Leu Trp Ala Thr Pro Asp His Lys Val 
65                  70                  75                  80  


Leu Thr Glu Tyr Gly Trp Arg Ala Ala Gly Glu Leu Arg Lys Gly Asp 
                85                  90                  95      


Arg Val Ala Gln Pro Arg Arg Phe Asp Gly Phe Gly Asp Ser Ala Pro 
            100                 105                 110         


Ile Pro Ala Arg Val Gln Ala Leu Ala Asp Ala Leu Asp Asp Lys Phe 
        115                 120                 125             


Leu His Asp Met Leu Ala Glu Glu Leu Arg Tyr Ser Val Ile Arg Glu 
    130                 135                 140                 


Val Leu Pro Thr Arg Arg Ala Arg Thr Phe Gly Leu Glu Val Glu Glu 
145                 150                 155                 160 


Leu His Thr Leu Val Ala Glu Gly Val Val Val His Asn 
                165                 170             


<210>  24
<211>  519
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; intein plus zinc binding motif sequence

<400>  24
ggagatggac atcatgccct cgcagagggc actcggatct tcgatccggt caccggtaca       60

acgcatcgca tcgaggatgt tgtcggtggg cgcaagccta ttcatgtcgt ggctgctgcc      120

aaggacggaa cgctgcatgc gcggcccgtg gtgtcctggt tcgaccaggg aacgcgggat      180

gtgatcgggt tgcggatcgc cggtggcgcc atcctgtggg cgacacccga tcacaaggtg      240

ctgacagagt acggctggcg tgccgccggg gaactccgca agggagacag ggtggcgcaa      300

ccgcgacgct tcgatggatt cggtgacagt gcgccgattc cggcgcgcgt gcaggcgctc      360

gcggatgccc tggatgacaa attcctgcac gacatgctgg cggaagaact ccgctattcc      420

gtgatccgag aagtgctgcc aacgcggcgg gcacgaacgt tcggcctcga ggtcgaggaa      480

ctgcacaccc tcgtcgccga aggggttgtt gtacacaac                             519


<210>  25
<211>  168
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; intein peptide

<400>  25

Cys Leu Ala Glu Gly Thr Arg Ile Phe Asp Pro Val Thr Gly Thr Thr 
1               5                   10                  15      


His Arg Ile Glu Asp Val Val Gly Gly Arg Lys Pro Ile His Val Val 
            20                  25                  30          


Ala Ala Ala Lys Asp Gly Thr Leu His Ala Arg Pro Val Val Ser Trp 
        35                  40                  45              


Phe Asp Gln Gly Thr Arg Asp Val Ile Gly Leu Arg Ile Ala Gly Gly 
    50                  55                  60                  


Ala Ile Leu Trp Ala Thr Pro Asp His Lys Val Leu Thr Glu Tyr Gly 
65                  70                  75                  80  


Trp Arg Ala Ala Gly Glu Leu Arg Lys Gly Asp Arg Val Ala Gln Pro 
                85                  90                  95      


Arg Arg Phe Asp Gly Phe Gly Asp Ser Ala Pro Ile Pro Ala Arg Val 
            100                 105                 110         


Gln Ala Leu Ala Asp Ala Leu Asp Asp Lys Phe Leu His Asp Met Leu 
        115                 120                 125             


Ala Glu Glu Leu Arg Tyr Ser Val Ile Arg Glu Val Leu Pro Thr Arg 
    130                 135                 140                 


Arg Ala Arg Thr Phe Gly Leu Glu Val Glu Glu Leu His Thr Leu Val 
145                 150                 155                 160 


Ala Glu Gly Val Val Val His Asn 
                165             


<210>  26
<211>  504
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; intein sequence

<400>  26
tgcctcgcag agggcactcg gatcttcgat ccggtcaccg gtacaacgca tcgcatcgag       60

gatgttgtcg gtgggcgcaa gcctattcat gtcgtggctg ctgccaagga cggaacgctg      120

catgcgcggc ccgtggtgtc ctggttcgac cagggaacgc gggatgtgat cgggttgcgg      180

atcgccggtg gcgccatcct gtgggcgaca cccgatcaca aggtgctgac agagtacggc      240

tggcgtgccg ccggggaact ccgcaaggga gacagggtgg cgcaaccgcg acgcttcgat      300

ggattcggtg acagtgcgcc gattccggcg cgcgtgcagg cgctcgcgga tgccctggat      360

gacaaattcc tgcacgacat gctggcggaa gaactccgct attccgtgat ccgagaagtg      420

ctgccaacgc ggcgggcacg aacgttcggc ctcgaggtcg aggaactgca caccctcgtc      480

gccgaagggg ttgttgtaca caac                                             504


<210>  27
<211>  173
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; intein peptide plus zinc binding motif

<400>  27

Gly Glu Gly His Gly Cys Leu Ala Glu Gly Thr Arg Ile Phe Asp Pro 
1               5                   10                  15      


Val Thr Gly Thr Thr His Arg Ile Glu Asp Val Val Gly Gly Arg Lys 
            20                  25                  30          


Pro Ile His Val Val Ala Ala Ala Lys Asp Gly Thr Leu His Ala Arg 
        35                  40                  45              


Pro Val Val Ser Trp Phe Asp Gln Gly Thr Arg Asp Val Ile Gly Leu 
    50                  55                  60                  


Arg Ile Ala Gly Gly Ala Ile Leu Trp Ala Thr Pro Asp His Lys Val 
65                  70                  75                  80  


Leu Thr Glu Tyr Gly Trp Arg Ala Ala Gly Glu Leu Arg Lys Gly Asp 
                85                  90                  95      


Arg Val Ala Gln Pro Arg Arg Phe Asp Gly Phe Gly Asp Ser Ala Pro 
            100                 105                 110         


Ile Pro Ala Arg Val Gln Ala Leu Ala Asp Ala Leu Asp Asp Lys Phe 
        115                 120                 125             


Leu His Asp Met Leu Ala Glu Glu Leu Arg Tyr Ser Val Ile Arg Glu 
    130                 135                 140                 


Val Leu Pro Thr Arg Arg Ala Arg Thr Phe Gly Leu Glu Val Glu Glu 
145                 150                 155                 160 


Leu His Thr Leu Val Ala Glu Gly Val Val Val His Asn 
                165                 170             


<210>  28
<211>  519
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; intein plus zinc binding motif sequence

<400>  28
ggagagggac atggatgcct cgcagagggc actcggatct tcgatccggt caccggtaca       60

acgcatcgca tcgaggatgt tgtcggtggg cgcaagccta ttcatgtcgt ggctgctgcc      120

aaggacggaa cgctgcatgc gcggcccgtg gtgtcctggt tcgaccaggg aacgcgggat      180

gtgatcgggt tgcggatcgc cggtggcgcc atcctgtggg cgacacccga tcacaaggtg      240

ctgacagagt acggctggcg tgccgccggg gaactccgca agggagacag ggtggcgcaa      300

ccgcgacgct tcgatggatt cggtgacagt gcgccgattc cggcgcgcgt gcaggcgctc      360

gcggatgccc tggatgacaa attcctgcac gacatgctgg cggaagaact ccgctattcc      420

gtgatccgag aagtgctgcc aacgcggcgg gcacgaacgt tcggcctcga ggtcgaggaa      480

ctgcacaccc tcgtcgccga aggggttgtt gtacacaac                             519


<210>  29
<211>  168
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; intein peptide

<400>  29

Cys Leu Ala Glu Gly Thr Arg Ile Phe Asp Pro Val Thr Gly Thr Thr 
1               5                   10                  15      


His Arg Ile Glu Asp Val Val Gly Gly Arg Lys Pro Ile His Val Val 
            20                  25                  30          


Ala Ala Ala Lys Asp Gly Thr Leu His Ala Arg Pro Val Val Ser Trp 
        35                  40                  45              


Phe Asp Gln Gly Thr Arg Asp Val Ile Gly Leu Arg Ile Ala Gly Gly 
    50                  55                  60                  


Ala Ile Leu Trp Ala Thr Pro Asp His Lys Val Leu Thr Glu Tyr Gly 
65                  70                  75                  80  


Trp Arg Ala Ala Gly Glu Leu Arg Lys Gly Asp Arg Val Ala Gln Pro 
                85                  90                  95      


Arg Arg Phe Asp Gly Phe Gly Asp Ser Ala Pro Ile Pro Ala Arg Val 
            100                 105                 110         


Gln Ala Leu Ala Asp Ala Leu Asp Asp Lys Phe Leu His Asp Met Leu 
        115                 120                 125             


Ala Glu Glu Leu Arg Tyr Ser Val Ile Arg Glu Val Leu Pro Thr Arg 
    130                 135                 140                 


Arg Ala Arg Thr Phe Gly Leu Glu Val Glu Glu Leu His Thr Leu Val 
145                 150                 155                 160 


Ala Glu Gly Val Val Val His Asn 
                165             


<210>  30
<211>  504
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; intein sequence

<400>  30
tgcctcgcag agggcactcg gatcttcgat ccggtcaccg gtacaacgca tcgcatcgag       60

gatgttgtcg gtgggcgcaa gcctattcat gtcgtggctg ctgccaagga cggaacgctg      120

catgcgcggc ccgtggtgtc ctggttcgac cagggaacgc gggatgtgat cgggttgcgg      180

atcgccggtg gcgccatcct gtgggcgaca cccgatcaca aggtgctgac agagtacggc      240

tggcgtgccg ccggggaact ccgcaaggga gacagggtgg cgcaaccgcg acgcttcgat      300

ggattcggtg acagtgcgcc gattccggcg cgcgtgcagg cgctcgcgga tgccctggat      360

gacaaattcc tgcacgacat gctggcggaa gaactccgct attccgtgat ccgagaagtg      420

ctgccaacgc ggcgggcacg aacgttcggc ctcgaggtcg aggaactgca caccctcgtc      480

gccgaagggg ttgttgtaca caac                                             504


<210>  31
<211>  173
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; intein peptide plus zinc binding motif

<400>  31

Gly Asp Gly His Gly Cys Leu Ala Glu Gly Thr Arg Ile Phe Asp Pro 
1               5                   10                  15      


Val Thr Gly Thr Thr His Arg Ile Glu Asp Val Val Gly Gly Arg Lys 
            20                  25                  30          


Pro Ile His Val Val Ala Ala Ala Lys Asp Gly Thr Leu His Ala Arg 
        35                  40                  45              


Pro Val Val Ser Trp Phe Asp Gln Gly Thr Arg Asp Val Ile Gly Leu 
    50                  55                  60                  


Arg Ile Ala Gly Gly Ala Ile Leu Trp Ala Thr Pro Asp His Lys Val 
65                  70                  75                  80  


Leu Thr Glu Tyr Gly Trp Arg Ala Ala Gly Glu Leu Arg Lys Gly Asp 
                85                  90                  95      


Arg Val Ala Gln Pro Arg Arg Phe Asp Gly Phe Gly Asp Ser Ala Pro 
            100                 105                 110         


Ile Pro Ala Arg Val Gln Ala Leu Ala Asp Ala Leu Asp Asp Lys Phe 
        115                 120                 125             


Leu His Asp Met Leu Ala Glu Glu Leu Arg Tyr Ser Val Ile Arg Glu 
    130                 135                 140                 


Val Leu Pro Thr Arg Arg Ala Arg Thr Phe Gly Leu Glu Val Glu Glu 
145                 150                 155                 160 


Leu His Thr Leu Val Ala Glu Gly Val Val Val His Asn 
                165                 170             


<210>  32
<211>  519
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; intein plus zinc binding motif sequence

<400>  32
ggagatggac atggatgcct cgcagagggc actcggatct tcgatccggt caccggtaca       60

acgcatcgca tcgaggatgt tgtcggtggg cgcaagccta ttcatgtcgt ggctgctgcc      120

aaggacggaa cgctgcatgc gcggcccgtg gtgtcctggt tcgaccaggg aacgcgggat      180

gtgatcgggt tgcggatcgc cggtggcgcc atcctgtggg cgacacccga tcacaaggtg      240

ctgacagagt acggctggcg tgccgccggg gaactccgca agggagacag ggtggcgcaa      300

ccgcgacgct tcgatggatt cggtgacagt gcgccgattc cggcgcgcgt gcaggcgctc      360

gcggatgccc tggatgacaa attcctgcac gacatgctgg cggaagaact ccgctattcc      420

gtgatccgag aagtgctgcc aacgcggcgg gcacgaacgt tcggcctcga ggtcgaggaa      480

ctgcacaccc tcgtcgccga aggggttgtt gtacacaac                             519


<210>  33
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; zinc binding domain and intein sequence

<400>  33
ggagagggac atcacctcgc agagggcact cggat                                  35


<210>  34
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; zinc binding motif and intein sequence

<400>  34
ggagagggac attgcctcgc agagggcact cggat                                  35


<210>  35
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; zinc binding motif and intein sequence

<400>  35
ggagagggac atcatgccct cgcagagggc actcg                                  35


<210>  36
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; zinc binding motif and intein sequence

<400>  36
ggagagggac atggatgcct cgcagagggc actcgg                                 36


<210>  37
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; zinc binding motif and intein sequence

<400>  37
ggagatggac atcatgccct cgcagagggc actcgga                                37


<210>  38
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic construct; zinc binding motif and intein sequence

<400>  38
ggagatggac atggatgcct cgcagagggc actcgga                                37


<210>  39
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; zinc binding motif

<400>  39

Gly Glu Gly His His Leu Ala Glu Gly Thr 
1               5                   10  


<210>  40
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; zinc binding motif

<400>  40

Gly Glu Gly His Cys Leu Ala Glu Gly Thr 
1               5                   10  


<210>  41
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; zinc binding motif

<400>  41

Gly Glu Gly His His Ala Leu Ala Glu Gly Thr 
1               5                   10      


<210>  42
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; zinc binding motif

<400>  42

Gly Glu Gly His Gly Cys Leu Ala Glu Gly Thr 
1               5                   10      


<210>  43
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; zinc binding motif

<400>  43

Gly Asp Gly His Gly Cys Leu Ala Glu Gly Thr 
1               5                   10      


<210>  44
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; zinc binding motif

<400>  44

Gly Asp Gly His Gly Cys Leu Ala Glu Gly Thr 
1               5                   10      


