                         SEQUENCE LISTING

<110>  Clariant Produkte (Deutschland) GmbH
 
<120>  Cell-free and minimized metabolic reaction cascades for the 
       production of chemicals

<130>  BT-28-I

<160>  69    

<170>  PatentIn version 3.5

<210>  1
<211>  1146
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Glucose dehydrogenase (EC 1.1.1.47), Sulfolobus solfataricus 
       DNA-sequence including C-terminal His-Tag

<400>  1
atgaaagcca ttattgtgaa acctccgaat gccggtgttc aggttaaaga tgtggatgaa       60

aaaaaactgg atagctatgg caaaattaaa attcgcacca tttataatgg tatttgcggc      120

accgatcgtg aaattgtgaa tggtaaactg accctgagca ccctgccgaa aggtaaagat      180

tttctggtgc tgggtcatga agcaattggt gttgtggaag aaagctatca tggttttagc      240

cagggtgatc tggttatgcc ggttaatcgt cgtggttgtg gtatttgtcg taattgtctg      300

gttggtcgtc cggatttttg tgaaaccggt gaatttggtg aagccggtat tcataaaatg      360

gatggcttta tgcgtgaatg gtggtatgat gatccgaaat atctggtgaa aattccgaaa      420

agcattgaag atattggtat tctggcacag ccgctggcag atattgaaaa atccattgaa      480

gaaattctgg aagtgcagaa acgtgttccg gtttggacct gtgatgatgg caccctgaat      540

tgtcgtaaag ttctggttgt tggcaccggt ccgattggtg ttctgtttac cctgctgttt      600

cgtacctatg gtctggaagt ttggatggca aatcgtcgtg aaccgaccga agttgaacag      660

accgttattg aagaaaccaa aaccaattat tataatagca gcaatggcta tgataaactg      720

aaagatagcg tgggcaaatt tgatgtgatt attgatgcaa ccggtgccga tgttaatatt      780

ctgggcaatg ttattccgct gctgggtcgt aatggtgttc tgggtctgtt tggttttagc      840

acctctggta gcgttccgct ggattataaa accctgcagg aaattgttca taccaataaa      900

accattattg gcctggtgaa tggtcagaaa ccgcattttc agcaggcagt tgttcatctg      960

gcaagctgga aaaccctgta tccgaaagca gcaaaaatgc tgattaccaa aaccgtgagc     1020

attaatgatg aaaaagaact gctgaaagtg ctgcgtgaaa aagaacatgg cgaaatcaaa     1080

attcgtattc tgtgggaaag cgggagacct gtgctgggca gcagccacca ccaccaccac     1140

cactaa                                                                1146


<210>  2
<211>  381
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Glucose dehydrogenase (EC 1.1.1.47), Sulfolobus solfataricus 
       Protein sequence including C-terminal His-Tag

<400>  2

Met Lys Ala Ile Ile Val Lys Pro Pro Asn Ala Gly Val Gln Val Lys 
1               5                   10                  15      


Asp Val Asp Glu Lys Lys Leu Asp Ser Tyr Gly Lys Ile Lys Ile Arg 
            20                  25                  30          


Thr Ile Tyr Asn Gly Ile Cys Gly Thr Asp Arg Glu Ile Val Asn Gly 
        35                  40                  45              


Lys Leu Thr Leu Ser Thr Leu Pro Lys Gly Lys Asp Phe Leu Val Leu 
    50                  55                  60                  


Gly His Glu Ala Ile Gly Val Val Glu Glu Ser Tyr His Gly Phe Ser 
65                  70                  75                  80  


Gln Gly Asp Leu Val Met Pro Val Asn Arg Arg Gly Cys Gly Ile Cys 
                85                  90                  95      


Arg Asn Cys Leu Val Gly Arg Pro Asp Phe Cys Glu Thr Gly Glu Phe 
            100                 105                 110         


Gly Glu Ala Gly Ile His Lys Met Asp Gly Phe Met Arg Glu Trp Trp 
        115                 120                 125             


Tyr Asp Asp Pro Lys Tyr Leu Val Lys Ile Pro Lys Ser Ile Glu Asp 
    130                 135                 140                 


Ile Gly Ile Leu Ala Gln Pro Leu Ala Asp Ile Glu Lys Ser Ile Glu 
145                 150                 155                 160 


Glu Ile Leu Glu Val Gln Lys Arg Val Pro Val Trp Thr Cys Asp Asp 
                165                 170                 175     


Gly Thr Leu Asn Cys Arg Lys Val Leu Val Val Gly Thr Gly Pro Ile 
            180                 185                 190         


Gly Val Leu Phe Thr Leu Leu Phe Arg Thr Tyr Gly Leu Glu Val Trp 
        195                 200                 205             


Met Ala Asn Arg Arg Glu Pro Thr Glu Val Glu Gln Thr Val Ile Glu 
    210                 215                 220                 


Glu Thr Lys Thr Asn Tyr Tyr Asn Ser Ser Asn Gly Tyr Asp Lys Leu 
225                 230                 235                 240 


Lys Asp Ser Val Gly Lys Phe Asp Val Ile Ile Asp Ala Thr Gly Ala 
                245                 250                 255     


Asp Val Asn Ile Leu Gly Asn Val Ile Pro Leu Leu Gly Arg Asn Gly 
            260                 265                 270         


Val Leu Gly Leu Phe Gly Phe Ser Thr Ser Gly Ser Val Pro Leu Asp 
        275                 280                 285             


Tyr Lys Thr Leu Gln Glu Ile Val His Thr Asn Lys Thr Ile Ile Gly 
    290                 295                 300                 


Leu Val Asn Gly Gln Lys Pro His Phe Gln Gln Ala Val Val His Leu 
305                 310                 315                 320 


Ala Ser Trp Lys Thr Leu Tyr Pro Lys Ala Ala Lys Met Leu Ile Thr 
                325                 330                 335     


Lys Thr Val Ser Ile Asn Asp Glu Lys Glu Leu Leu Lys Val Leu Arg 
            340                 345                 350         


Glu Lys Glu His Gly Glu Ile Lys Ile Arg Ile Leu Trp Glu Ser Gly 
        355                 360                 365             


Arg Pro Val Leu Gly Ser Ser His His His His His His 
    370                 375                 380     


<210>  3
<211>  1689
<212>  DNA
<213>  Sulfolobus solfataricus

<400>  3
atgcctgcaa aactgaatag cccgagccgt tatcatggta tttataatgc accgcatcgt       60

gcatttctgc gtagcgttgg tctgaccgat gaagaaattg gtaaaccgct ggttgcaatt      120

gccaccgcat ggtctgaagc cggtccgtgt aattttcata ccctggcact ggcacgtgtt      180

gcaaaagaag gcaccaaaga agccggtctg tctccgctgg catttccgac catggttgtg      240

aatgataata ttggcatggg tagcgaaggt atgcgttata gcctggttag ccgtgatctg      300

attgcagata tggttgaagc acagtttaat gcccatgcat ttgatggtct ggttggtatt      360

ggtggttgtg ataaaaccac accgggtatt ctgatggcaa tggcacgtct gaatgttccg      420

agcatttata tttatggtgg tagcgcagaa ccgggttatt ttatgggtaa acgcctgacc      480

attgaagatg ttcatgaagc cattggtgca tatctggcaa aacgcattac cgaaaatgaa      540

ctgtatgaaa ttgaaaaacg tgcacatccg accctgggca cctgtagcgg tctgtttacc      600

gcaaatacca tgggtagcat gagcgaagca ctgggtatgg cactgcctgg tagcgcatct      660

ccgaccgcaa ccagcagccg tcgtgttatg tatgttaaag aaaccggtaa agccctgggt      720

agcctgattg aaaatggcat taaaagccgt gaaattctga cctttgaagc ctttgaaaat      780

gcaattacaa ccctgatggc gatgggtggt agcaccaatg cagttctgca tctgctggca      840

attgcttatg aagccggtgt taaactgacc ctggatgatt ttaatcgcat tagcaaacgc      900

accccgtata ttgcaagcat gaaaccgggt ggtgattatg ttatggccga tctggatgaa      960

gttggtggtg ttccggttgt tctgaaaaaa ctgctggatg ccggtctgct gcatggtgat     1020

gttctgaccg ttaccggtaa aaccatgaaa cagaatctgg aacagtataa atatccgaat     1080

gtgccgcata gccatattgt tcgtgatgtg aaaaatccga ttaaaccgcg tggtggtatt     1140

gttattctga aaggtagcct ggcaccggaa ggtgcagtta ttaaagttgc agccaccaat     1200

gtggttaaat ttgaaggcaa agccaaagtg tataatagcg aagatgatgc ctttaaaggt     1260

gttcagagcg gtgaagttag cgaaggtgaa gtggtgatta ttcgctatga aggtccgaaa     1320

ggtgcaccgg gtatgccgga aatgctgcgc gttaccgcag cgattatggg tgccggtctg     1380

aataatgttg cactggttac cgatggtcgt tttagcggtg caacccgtgg tccgatggtt     1440

ggtcatgttg caccggaagc aatggttggt ggtccgattg caattgttga agatggcgat     1500

accattgtga ttgatgtgga aagcgaacgt ctggatctga aactgagcga agaagaaatt     1560

aaaaatcgcc tgaaacgttg gagcccgccg tcaccgcgtt ataaaagcgg tctgctggca     1620

aaatatgcaa gcctggtttc tcaggcaagc atgggtgcag ttacccgtcc ggcaagagac     1680

cttaattaa                                                             1689


<210>  4
<211>  558
<212>  PRT
<213>  Sulfolobus solfataricus

<400>  4

Met Pro Ala Lys Leu Asn Ser Pro Ser Arg Tyr His Gly Ile Tyr Asn 
1               5                   10                  15      


Ala Pro His Arg Ala Phe Leu Arg Ser Val Gly Leu Thr Asp Glu Glu 
            20                  25                  30          


Ile Gly Lys Pro Leu Val Ala Ile Ala Thr Ala Trp Ser Glu Ala Gly 
        35                  40                  45              


Pro Cys Asn Phe His Thr Leu Ala Leu Ala Arg Val Ala Lys Glu Gly 
    50                  55                  60                  


Thr Lys Glu Ala Gly Leu Ser Pro Leu Ala Phe Pro Thr Met Val Val 
65                  70                  75                  80  


Asn Asp Asn Ile Gly Met Gly Ser Glu Gly Met Arg Tyr Ser Leu Val 
                85                  90                  95      


Ser Arg Asp Leu Ile Ala Asp Met Val Glu Ala Gln Phe Asn Ala His 
            100                 105                 110         


Ala Phe Asp Gly Leu Val Gly Ile Gly Gly Cys Asp Lys Thr Thr Pro 
        115                 120                 125             


Gly Ile Leu Met Ala Met Ala Arg Leu Asn Val Pro Ser Ile Tyr Ile 
    130                 135                 140                 


Tyr Gly Gly Ser Ala Glu Pro Gly Tyr Phe Met Gly Lys Arg Leu Thr 
145                 150                 155                 160 


Ile Glu Asp Val His Glu Ala Ile Gly Ala Tyr Leu Ala Lys Arg Ile 
                165                 170                 175     


Thr Glu Asn Glu Leu Tyr Glu Ile Glu Lys Arg Ala His Pro Thr Leu 
            180                 185                 190         


Gly Thr Cys Ser Gly Leu Phe Thr Ala Asn Thr Met Gly Ser Met Ser 
        195                 200                 205             


Glu Ala Leu Gly Met Ala Leu Pro Gly Ser Ala Ser Pro Thr Ala Thr 
    210                 215                 220                 


Ser Ser Arg Arg Val Met Tyr Val Lys Glu Thr Gly Lys Ala Leu Gly 
225                 230                 235                 240 


Ser Leu Ile Glu Asn Gly Ile Lys Ser Arg Glu Ile Leu Thr Phe Glu 
                245                 250                 255     


Ala Phe Glu Asn Ala Ile Thr Thr Leu Met Ala Met Gly Gly Ser Thr 
            260                 265                 270         


Asn Ala Val Leu His Leu Leu Ala Ile Ala Tyr Glu Ala Gly Val Lys 
        275                 280                 285             


Leu Thr Leu Asp Asp Phe Asn Arg Ile Ser Lys Arg Thr Pro Tyr Ile 
    290                 295                 300                 


Ala Ser Met Lys Pro Gly Gly Asp Tyr Val Met Ala Asp Leu Asp Glu 
305                 310                 315                 320 


Val Gly Gly Val Pro Val Val Leu Lys Lys Leu Leu Asp Ala Gly Leu 
                325                 330                 335     


Leu His Gly Asp Val Leu Thr Val Thr Gly Lys Thr Met Lys Gln Asn 
            340                 345                 350         


Leu Glu Gln Tyr Lys Tyr Pro Asn Val Pro His Ser His Ile Val Arg 
        355                 360                 365             


Asp Val Lys Asn Pro Ile Lys Pro Arg Gly Gly Ile Val Ile Leu Lys 
    370                 375                 380                 


Gly Ser Leu Ala Pro Glu Gly Ala Val Ile Lys Val Ala Ala Thr Asn 
385                 390                 395                 400 


Val Val Lys Phe Glu Gly Lys Ala Lys Val Tyr Asn Ser Glu Asp Asp 
                405                 410                 415     


Ala Phe Lys Gly Val Gln Ser Gly Glu Val Ser Glu Gly Glu Val Val 
            420                 425                 430         


Ile Ile Arg Tyr Glu Gly Pro Lys Gly Ala Pro Gly Met Pro Glu Met 
        435                 440                 445             


Leu Arg Val Thr Ala Ala Ile Met Gly Ala Gly Leu Asn Asn Val Ala 
    450                 455                 460                 


Leu Val Thr Asp Gly Arg Phe Ser Gly Ala Thr Arg Gly Pro Met Val 
465                 470                 475                 480 


Gly His Val Ala Pro Glu Ala Met Val Gly Gly Pro Ile Ala Ile Val 
                485                 490                 495     


Glu Asp Gly Asp Thr Ile Val Ile Asp Val Glu Ser Glu Arg Leu Asp 
            500                 505                 510         


Leu Lys Leu Ser Glu Glu Glu Ile Lys Asn Arg Leu Lys Arg Trp Ser 
        515                 520                 525             


Pro Pro Ser Pro Arg Tyr Lys Ser Gly Leu Leu Ala Lys Tyr Ala Ser 
    530                 535                 540                 


Leu Val Ser Gln Ala Ser Met Gly Ala Val Thr Arg Pro Ala 
545                 550                 555             


<210>  5
<211>  867
<212>  DNA
<213>  Sulfolobus acidocaldarius

<400>  5
atggaaatta ttagcccgat tattaccccg tttgataaac agggtaaagt gaatgttgat       60

gccctgaaaa cccatgcaaa aaatctgctg gaaaaaggca ttgatgccat ttttgttaat      120

ggcaccaccg gtctgggtcc ggcactgagc aaagatgaaa aacgccagaa tctgaatgca      180

ctgtatgatg tgacccataa actgattttt caggtgggta gcctgaatct gaatgatgtt      240

atggaactgg tgaaatttag caatgaaatg gatattctgg gtgttagcag ccatagcccg      300

tattattttc cgcgtctgcc ggaaaaattt ctggccaaat attatgaaga aattgcccgt      360

attagcagcc attccctgta tatttataat tatccggcag ccaccggtta tgatattcca      420

ccgagcattc tgaaaagcct gccggtgaaa ggtattaaag ataccaatca ggatctggca      480

catagcctgg aatacaaact gaatctgccg ggtgtgaaag tttataatgg cagcaatacc      540

ctgatttatt atagcctgct gagcctggat ggtgttgttg caagctttac caatttcatt      600

ccggaagtga ttgtgaaaca gcgcgatctg attaaacagg gcaaactgga tgatgcactg      660

cgtctgcagg aactgattaa tcgtctggca gatattctgc gtaaatatgg tagcattagc      720

gccatttatg tgctggtgaa tgaatttcag ggttatgatg ttggttatcc gcgtccgccg      780

atttttccgc tgaccgatga agaagcactg agcctgaaac gtgaaattga accgctgaaa      840

cgcaaaattc aggaactggt tcattaa                                          867


<210>  6
<211>  288
<212>  PRT
<213>  Sulfolobus acidocaldarius

<400>  6

Met Glu Ile Ile Ser Pro Ile Ile Thr Pro Phe Asp Lys Gln Gly Lys 
1               5                   10                  15      


Val Asn Val Asp Ala Leu Lys Thr His Ala Lys Asn Leu Leu Glu Lys 
            20                  25                  30          


Gly Ile Asp Ala Ile Phe Val Asn Gly Thr Thr Gly Leu Gly Pro Ala 
        35                  40                  45              


Leu Ser Lys Asp Glu Lys Arg Gln Asn Leu Asn Ala Leu Tyr Asp Val 
    50                  55                  60                  


Thr His Lys Leu Ile Phe Gln Val Gly Ser Leu Asn Leu Asn Asp Val 
65                  70                  75                  80  


Met Glu Leu Val Lys Phe Ser Asn Glu Met Asp Ile Leu Gly Val Ser 
                85                  90                  95      


Ser His Ser Pro Tyr Tyr Phe Pro Arg Leu Pro Glu Lys Phe Leu Ala 
            100                 105                 110         


Lys Tyr Tyr Glu Glu Ile Ala Arg Ile Ser Ser His Ser Leu Tyr Ile 
        115                 120                 125             


Tyr Asn Tyr Pro Ala Ala Thr Gly Tyr Asp Ile Pro Pro Ser Ile Leu 
    130                 135                 140                 


Lys Ser Leu Pro Val Lys Gly Ile Lys Asp Thr Asn Gln Asp Leu Ala 
145                 150                 155                 160 


His Ser Leu Glu Tyr Lys Leu Asn Leu Pro Gly Val Lys Val Tyr Asn 
                165                 170                 175     


Gly Ser Asn Thr Leu Ile Tyr Tyr Ser Leu Leu Ser Leu Asp Gly Val 
            180                 185                 190         


Val Ala Ser Phe Thr Asn Phe Ile Pro Glu Val Ile Val Lys Gln Arg 
        195                 200                 205             


Asp Leu Ile Lys Gln Gly Lys Leu Asp Asp Ala Leu Arg Leu Gln Glu 
    210                 215                 220                 


Leu Ile Asn Arg Leu Ala Asp Ile Leu Arg Lys Tyr Gly Ser Ile Ser 
225                 230                 235                 240 


Ala Ile Tyr Val Leu Val Asn Glu Phe Gln Gly Tyr Asp Val Gly Tyr 
                245                 250                 255     


Pro Arg Pro Pro Ile Phe Pro Leu Thr Asp Glu Glu Ala Leu Ser Leu 
            260                 265                 270         


Lys Arg Glu Ile Glu Pro Leu Lys Arg Lys Ile Gln Glu Leu Val His 
        275                 280                 285             


<210>  7
<211>  1527
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldehyde Dehydrogenase (EC 1.2.1.3), Thermoplasma acidophilum, 
       wildtype enzyme DNA-sequence including C-terminal His-Tag

<400>  7
atggatacca aactgtatat tgatggccag tgggttaata gcagcagcgg taaaaccgtt       60

gataaatatt ctccggttac cggtcaggtt attggtcgtt ttgaagcagc aacccgtgat      120

gatgttgatc gtgcaattga tgcagcagaa gatgcatttt gggcctggaa tgatctgggt      180

agcgttgaac gcagcaaaat tatttatcgt gccaaagaac tgattgaaaa aaatcgtgcc      240

gaactggaaa atattattat ggaagaaaat ggcaaaccgg tgaaagaagc caaagaagaa      300

gtcgacggcg tcattgatca gattcagtat tatgcagaat gggcacgtaa actgaatggt      360

gaagttgttg aaggcaccag cagccatcgt aaaatttttc agtataaagt gccgtatggt      420

attgttgttg cactgacccc gtggaatttt ccggcaggca tggttgcccg taaactggca      480

ccggcactgc tgaccggtaa taccgttgtt ctgaaaccga gcagcgatac accgggtagc      540

gcagaatgga ttgtgcgtaa atttgttgaa gccggtgttc cgaaaggtgt gctgaatttt      600

attaccggtc gtggtagcga aattggcgat tacattgtgg aacataaaaa agtcaatctg      660

attaccatga ccggtagcac cgcaacaggt cagcgcatta tgcagaaagc aagcgcaaat      720

atggcaaaac tgattctgga actgggtggt aaagcaccgt ttatggtttg gaaagatgcc      780

gatatggata atgcactgaa aaccctgctg tgggcaaaat attggaatgc cggtcagagc      840

tgtattgcag cagaacgtct gtatgtgcat gaagatattt atgatacctt tatgagccgt      900

tttgttgaac tgagccgcaa actggcactg ggtgatccga aaaatgcaga tatgggtccg      960

ctgattaata aaggtgcact gcaggcaacc agcgaaattg ttgaagaagc gaaagaatct     1020

ggcgcaaaaa ttctgtttgg tggtagccag ccgagcctga gcggtccgta tcgtaatggc     1080

tatttttttc tgccgaccat tattggtaat gcggatcaga aaagcaaaat ctttcaggaa     1140

gaaatttttg caccggttat tggtgcacgt aaaattagca gcgtggaaga aatgtatgat     1200

ctggccaatg atagcaaata tggtctggcc agctacctgt ttaccaaaga tccgaatatc     1260

atttttgaag ccagcgaacg tattcgtttt ggtgaactgt atgtgaatat gccgggtccg     1320

gaagcaagcc agggttatca caccggtttt cgtatgacag gtcaggcagg cgaaggttct     1380

aaatatggca ttagcgaata tctgaaactg aaaaatattt atgtggatta tagcggcaaa     1440

ccgctgcata ttaataccgt tcgtgatgac ctgtttcaga gcgggagacc tgtgctgggc     1500

agcagccacc accaccacca ccactaa                                         1527


<210>  8
<211>  508
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldehyde Dehydrogenase (EC 1.2.1.3), Thermoplasma acidophilum, 
       wildtype enzyme Protein sequence including C-terminal His-Tag

<400>  8

Met Asp Thr Lys Leu Tyr Ile Asp Gly Gln Trp Val Asn Ser Ser Ser 
1               5                   10                  15      


Gly Lys Thr Val Asp Lys Tyr Ser Pro Val Thr Gly Gln Val Ile Gly 
            20                  25                  30          


Arg Phe Glu Ala Ala Thr Arg Asp Asp Val Asp Arg Ala Ile Asp Ala 
        35                  40                  45              


Ala Glu Asp Ala Phe Trp Ala Trp Asn Asp Leu Gly Ser Val Glu Arg 
    50                  55                  60                  


Ser Lys Ile Ile Tyr Arg Ala Lys Glu Leu Ile Glu Lys Asn Arg Ala 
65                  70                  75                  80  


Glu Leu Glu Asn Ile Ile Met Glu Glu Asn Gly Lys Pro Val Lys Glu 
                85                  90                  95      


Ala Lys Glu Glu Val Asp Gly Val Ile Asp Gln Ile Gln Tyr Tyr Ala 
            100                 105                 110         


Glu Trp Ala Arg Lys Leu Asn Gly Glu Val Val Glu Gly Thr Ser Ser 
        115                 120                 125             


His Arg Lys Ile Phe Gln Tyr Lys Val Pro Tyr Gly Ile Val Val Ala 
    130                 135                 140                 


Leu Thr Pro Trp Asn Phe Pro Ala Gly Met Val Ala Arg Lys Leu Ala 
145                 150                 155                 160 


Pro Ala Leu Leu Thr Gly Asn Thr Val Val Leu Lys Pro Ser Ser Asp 
                165                 170                 175     


Thr Pro Gly Ser Ala Glu Trp Ile Val Arg Lys Phe Val Glu Ala Gly 
            180                 185                 190         


Val Pro Lys Gly Val Leu Asn Phe Ile Thr Gly Arg Gly Ser Glu Ile 
        195                 200                 205             


Gly Asp Tyr Ile Val Glu His Lys Lys Val Asn Leu Ile Thr Met Thr 
    210                 215                 220                 


Gly Ser Thr Ala Thr Gly Gln Arg Ile Met Gln Lys Ala Ser Ala Asn 
225                 230                 235                 240 


Met Ala Lys Leu Ile Leu Glu Leu Gly Gly Lys Ala Pro Phe Met Val 
                245                 250                 255     


Trp Lys Asp Ala Asp Met Asp Asn Ala Leu Lys Thr Leu Leu Trp Ala 
            260                 265                 270         


Lys Tyr Trp Asn Ala Gly Gln Ser Cys Ile Ala Ala Glu Arg Leu Tyr 
        275                 280                 285             


Val His Glu Asp Ile Tyr Asp Thr Phe Met Ser Arg Phe Val Glu Leu 
    290                 295                 300                 


Ser Arg Lys Leu Ala Leu Gly Asp Pro Lys Asn Ala Asp Met Gly Pro 
305                 310                 315                 320 


Leu Ile Asn Lys Gly Ala Leu Gln Ala Thr Ser Glu Ile Val Glu Glu 
                325                 330                 335     


Ala Lys Glu Ser Gly Ala Lys Ile Leu Phe Gly Gly Ser Gln Pro Ser 
            340                 345                 350         


Leu Ser Gly Pro Tyr Arg Asn Gly Tyr Phe Phe Leu Pro Thr Ile Ile 
        355                 360                 365             


Gly Asn Ala Asp Gln Lys Ser Lys Ile Phe Gln Glu Glu Ile Phe Ala 
    370                 375                 380                 


Pro Val Ile Gly Ala Arg Lys Ile Ser Ser Val Glu Glu Met Tyr Asp 
385                 390                 395                 400 


Leu Ala Asn Asp Ser Lys Tyr Gly Leu Ala Ser Tyr Leu Phe Thr Lys 
                405                 410                 415     


Asp Pro Asn Ile Ile Phe Glu Ala Ser Glu Arg Ile Arg Phe Gly Glu 
            420                 425                 430         


Leu Tyr Val Asn Met Pro Gly Pro Glu Ala Ser Gln Gly Tyr His Thr 
        435                 440                 445             


Gly Phe Arg Met Thr Gly Gln Ala Gly Glu Gly Ser Lys Tyr Gly Ile 
    450                 455                 460                 


Ser Glu Tyr Leu Lys Leu Lys Asn Ile Tyr Val Asp Tyr Ser Gly Lys 
465                 470                 475                 480 


Pro Leu His Ile Asn Thr Val Arg Asp Asp Leu Phe Gln Ser Gly Arg 
                485                 490                 495     


Pro Val Leu Gly Ser Ser His His His His His His 
            500                 505             


<210>  9
<211>  1527
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Aldehyde Dehydrogenase (EC 1.2.1.3), Thermoplasma acidophilum, 
       variant F34M-Y399C-S405N DNA-sequence including C-terminal 
       His-Tag

<400>  9
atggatacca aactgtatat tgatggccag tgggttaata gcagcagcgg taaaaccgtt       60

gataaatatt ctccggttac cggtcaggtt attggtcgta tggaagcagc aacccgtgat      120

gatgttgatc gtgcaattga tgcagcagaa gatgcatttt gggcctggaa tgatctgggt      180

agcgttgaac gcagcaaaat tatttatcgt gccaaagaac tgattgaaaa aaatcgtgcc      240

gaactggaaa atattattat ggaagaaaat ggcaaaccgg tgaaagaagc caaagaagaa      300

gtcgacggcg tcattgatca gattcagtat tatgcagaat gggcacgtaa actgaatggt      360

gaagttgttg aaggcaccag cagccatcgt aaaatttttc agtataaagt gccgtatggt      420

attgttgttg cactgacccc gtggaatttt ccggcaggca tggttgcccg taaactggca      480

ccggcactgc tgaccggtaa taccgttgtt ctgaaaccga gcagcgatac accgggtagc      540

gcagaatgga ttgtgcgtaa atttgttgaa gccggtgttc cgaaaggtgt gctgaatttt      600

attaccggtc gtggtagcga aattggcgat tacattgtgg aacataaaaa agtcaatctg      660

attaccatga ccggtagcac cgcaacaggt cagcgcatta tgcagaaagc aagcgcaaat      720

atggcaaaac tgattctgga actgggtggt aaagcaccgt ttatggtttg gaaagatgcc      780

gatatggata atgcactgaa aaccctgctg tgggcaaaat attggaatgc cggtcagagc      840

tgtattgcag cagaacgtct gtatgtgcat gaagatattt atgatacctt tatgagccgt      900

tttgttgaac tgagccgcaa actggcactg ggtgatccga aaaatgcaga tatgggtccg      960

ctgattaata aaggtgcact gcaggcaacc agcgaaattg ttgaagaagc gaaagaatct     1020

ggcgcaaaaa ttctgtttgg tggtagccag ccgagcctga gcggtccgta tcgtaatggc     1080

tatttttttc tgccgaccat tattggtaat gcggatcaga aaagcaaaat ctttcaggaa     1140

gaaatttttg caccggttat tggtgcacgt aaaattagca gcgtggaaga aatgtgtgat     1200

ctggccaatg ataataaata tggtctggcc agctacctgt ttaccaaaga tccgaatatc     1260

atttttgaag ccagcgaacg tattcgtttt ggtgaactgt atgtgaatat gccgggtccg     1320

gaagcaagcc agggttatca caccggtttt cgtatgacag gtcaggcagg cgaaggttct     1380

aaatatggca ttagcgaata tctgaaactg aaaaatattt atgtggatta tagcggcaaa     1440

ccgctgcata ttaataccgt tcgtgatgac ctgtttcaga gcgggagacc tgtgctgggc     1500

agcagccacc accaccacca ccactaa                                         1527


<210>  10
<211>  508
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Aldehyde Dehydrogenase (EC 1.2.1.3), Thermoplasma acidophilum, 
       variant F34M-Y399C-S405N Protein sequence including C-terminal 
       His-Tag

<400>  10

Met Asp Thr Lys Leu Tyr Ile Asp Gly Gln Trp Val Asn Ser Ser Ser 
1               5                   10                  15      


Gly Lys Thr Val Asp Lys Tyr Ser Pro Val Thr Gly Gln Val Ile Gly 
            20                  25                  30          


Arg Met Glu Ala Ala Thr Arg Asp Asp Val Asp Arg Ala Ile Asp Ala 
        35                  40                  45              


Ala Glu Asp Ala Phe Trp Ala Trp Asn Asp Leu Gly Ser Val Glu Arg 
    50                  55                  60                  


Ser Lys Ile Ile Tyr Arg Ala Lys Glu Leu Ile Glu Lys Asn Arg Ala 
65                  70                  75                  80  


Glu Leu Glu Asn Ile Ile Met Glu Glu Asn Gly Lys Pro Val Lys Glu 
                85                  90                  95      


Ala Lys Glu Glu Val Asp Gly Val Ile Asp Gln Ile Gln Tyr Tyr Ala 
            100                 105                 110         


Glu Trp Ala Arg Lys Leu Asn Gly Glu Val Val Glu Gly Thr Ser Ser 
        115                 120                 125             


His Arg Lys Ile Phe Gln Tyr Lys Val Pro Tyr Gly Ile Val Val Ala 
    130                 135                 140                 


Leu Thr Pro Trp Asn Phe Pro Ala Gly Met Val Ala Arg Lys Leu Ala 
145                 150                 155                 160 


Pro Ala Leu Leu Thr Gly Asn Thr Val Val Leu Lys Pro Ser Ser Asp 
                165                 170                 175     


Thr Pro Gly Ser Ala Glu Trp Ile Val Arg Lys Phe Val Glu Ala Gly 
            180                 185                 190         


Val Pro Lys Gly Val Leu Asn Phe Ile Thr Gly Arg Gly Ser Glu Ile 
        195                 200                 205             


Gly Asp Tyr Ile Val Glu His Lys Lys Val Asn Leu Ile Thr Met Thr 
    210                 215                 220                 


Gly Ser Thr Ala Thr Gly Gln Arg Ile Met Gln Lys Ala Ser Ala Asn 
225                 230                 235                 240 


Met Ala Lys Leu Ile Leu Glu Leu Gly Gly Lys Ala Pro Phe Met Val 
                245                 250                 255     


Trp Lys Asp Ala Asp Met Asp Asn Ala Leu Lys Thr Leu Leu Trp Ala 
            260                 265                 270         


Lys Tyr Trp Asn Ala Gly Gln Ser Cys Ile Ala Ala Glu Arg Leu Tyr 
        275                 280                 285             


Val His Glu Asp Ile Tyr Asp Thr Phe Met Ser Arg Phe Val Glu Leu 
    290                 295                 300                 


Ser Arg Lys Leu Ala Leu Gly Asp Pro Lys Asn Ala Asp Met Gly Pro 
305                 310                 315                 320 


Leu Ile Asn Lys Gly Ala Leu Gln Ala Thr Ser Glu Ile Val Glu Glu 
                325                 330                 335     


Ala Lys Glu Ser Gly Ala Lys Ile Leu Phe Gly Gly Ser Gln Pro Ser 
            340                 345                 350         


Leu Ser Gly Pro Tyr Arg Asn Gly Tyr Phe Phe Leu Pro Thr Ile Ile 
        355                 360                 365             


Gly Asn Ala Asp Gln Lys Ser Lys Ile Phe Gln Glu Glu Ile Phe Ala 
    370                 375                 380                 


Pro Val Ile Gly Ala Arg Lys Ile Ser Ser Val Glu Glu Met Cys Asp 
385                 390                 395                 400 


Leu Ala Asn Asp Asn Lys Tyr Gly Leu Ala Ser Tyr Leu Phe Thr Lys 
                405                 410                 415     


Asp Pro Asn Ile Ile Phe Glu Ala Ser Glu Arg Ile Arg Phe Gly Glu 
            420                 425                 430         


Leu Tyr Val Asn Met Pro Gly Pro Glu Ala Ser Gln Gly Tyr His Thr 
        435                 440                 445             


Gly Phe Arg Met Thr Gly Gln Ala Gly Glu Gly Ser Lys Tyr Gly Ile 
    450                 455                 460                 


Ser Glu Tyr Leu Lys Leu Lys Asn Ile Tyr Val Asp Tyr Ser Gly Lys 
465                 470                 475                 480 


Pro Leu His Ile Asn Thr Val Arg Asp Asp Leu Phe Gln Ser Gly Arg 
                485                 490                 495     


Pro Val Leu Gly Ser Ser His His His His His His 
            500                 505             


<210>  11
<211>  1719
<212>  DNA
<213>  Bacillus subtilis

<400>  11
atgctgacca aagcaaccaa agaacagaaa agcctggtga aaaatcgtgg tgcagaactg       60

gttgttgatt gtctggttga acagggtgtt acccatgttt ttggtattcc gggtgcaaaa      120

attgatgcag tttttgatgc cctgcaggat aaaggtccgg aaattattgt tgcacgccat      180

gaacagaatg cagcatttat ggcacaggca gttggtcgtc tgaccggtaa accgggtgtt      240

gttctggtta ccagcggtcc gggtgcaagc aatctggcaa ccggtctgct gaccgcaaat      300

accgaaggtg atccggttgt tgcactggca ggtaatgtta ttcgtgcaga tcgtctgaaa      360

cgtacccatc agagcctgga taatgcagca ctgtttcagc cgattaccaa atattcagtt      420

gaagtgcagg atgtgaaaaa tattccggaa gcagttacca atgcctttcg tattgcaagc      480

gcaggtcagg caggcgcagc atttgttagc tttccgcagg atgttgttaa tgaagtgacc      540

aataccaaaa atgttcgtgc agttgcagca ccgaaactgg gtccggcagc agatgatgca      600

attagcgcag caattgcaaa aattcagacc gcaaaactgc cggttgttct ggtgggtatg      660

aaaggtggtc gtccggaagc aattaaagca gttcgtaaac tgctgaaaaa agttcagctg      720

ccgtttgttg aaacctatca ggcagcaggc accctgagcc gtgatctgga agatcagtat      780

tttggtcgta ttggtctgtt tcgtaatcag cctggtgatc tgctgctgga acaggcagat      840

gttgttctga ccattggtta tgatccgatt gagtatgatc cgaaattttg gaacattaat      900

ggcgatcgca ccattattca cctggatgaa attattgccg atatcgatca tgcatatcag      960

ccggatctgg aactgattgg tgatattccg agcaccatta accatattga acatgatgcc     1020

gtgaaagtgg aatttgcaga acgtgaacag aaaattctga gcgatctgaa acagtatatg     1080

catgaaggtg aacaggttcc ggcagattgg aaaagcgatc gtgcacatcc gctggaaatt     1140

gttaaagaac tgcgtaatgc cgtggatgat catgttaccg ttacctgtga tattggtagc     1200

catgcaattt ggatgagccg ttattttcgt agctatgaac cgctgaccct gatgattagc     1260

aatggtatgc agaccctggg tgttgcactg ccgtgggcaa ttggtgcaag cctggttaaa     1320

ccgggtgaaa aagttgttag cgttagcggt gatggtggtt ttctgtttag cgcaatggaa     1380

ctggaaaccg cagttcgtct gaaagcaccg attgttcata ttgtttggaa cgatagcacc     1440

tatgatatgg ttgcatttca gcagctgaaa aaatataatc gtaccagcgc agtggatttt     1500

ggcaatattg acattgtgaa atacgccgaa agctttggtg ccaccggtct gcgtgttgaa     1560

agtccggatc agctggcaga tgttctgcgt cagggtatga atgcagaagg tccggttatt     1620

attgatgttc cggttgatta tagcgataac attaatctgg ccagcgataa actgccgaaa     1680

gaatttggtg aactgatgaa aaccaaagcc ctgttataa                            1719


<210>  12
<211>  572
<212>  PRT
<213>  Bacillus subtilis

<400>  12

Met Leu Thr Lys Ala Thr Lys Glu Gln Lys Ser Leu Val Lys Asn Arg 
1               5                   10                  15      


Gly Ala Glu Leu Val Val Asp Cys Leu Val Glu Gln Gly Val Thr His 
            20                  25                  30          


Val Phe Gly Ile Pro Gly Ala Lys Ile Asp Ala Val Phe Asp Ala Leu 
        35                  40                  45              


Gln Asp Lys Gly Pro Glu Ile Ile Val Ala Arg His Glu Gln Asn Ala 
    50                  55                  60                  


Ala Phe Met Ala Gln Ala Val Gly Arg Leu Thr Gly Lys Pro Gly Val 
65                  70                  75                  80  


Val Leu Val Thr Ser Gly Pro Gly Ala Ser Asn Leu Ala Thr Gly Leu 
                85                  90                  95      


Leu Thr Ala Asn Thr Glu Gly Asp Pro Val Val Ala Leu Ala Gly Asn 
            100                 105                 110         


Val Ile Arg Ala Asp Arg Leu Lys Arg Thr His Gln Ser Leu Asp Asn 
        115                 120                 125             


Ala Ala Leu Phe Gln Pro Ile Thr Lys Tyr Ser Val Glu Val Gln Asp 
    130                 135                 140                 


Val Lys Asn Ile Pro Glu Ala Val Thr Asn Ala Phe Arg Ile Ala Ser 
145                 150                 155                 160 


Ala Gly Gln Ala Gly Ala Ala Phe Val Ser Phe Pro Gln Asp Val Val 
                165                 170                 175     


Asn Glu Val Thr Asn Thr Lys Asn Val Arg Ala Val Ala Ala Pro Lys 
            180                 185                 190         


Leu Gly Pro Ala Ala Asp Asp Ala Ile Ser Ala Ala Ile Ala Lys Ile 
        195                 200                 205             


Gln Thr Ala Lys Leu Pro Val Val Leu Val Gly Met Lys Gly Gly Arg 
    210                 215                 220                 


Pro Glu Ala Ile Lys Ala Val Arg Lys Leu Leu Lys Lys Val Gln Leu 
225                 230                 235                 240 


Pro Phe Val Glu Thr Tyr Gln Ala Ala Gly Thr Leu Ser Arg Asp Leu 
                245                 250                 255     


Glu Asp Gln Tyr Phe Gly Arg Ile Gly Leu Phe Arg Asn Gln Pro Gly 
            260                 265                 270         


Asp Leu Leu Leu Glu Gln Ala Asp Val Val Leu Thr Ile Gly Tyr Asp 
        275                 280                 285             


Pro Ile Glu Tyr Asp Pro Lys Phe Trp Asn Ile Asn Gly Asp Arg Thr 
    290                 295                 300                 


Ile Ile His Leu Asp Glu Ile Ile Ala Asp Ile Asp His Ala Tyr Gln 
305                 310                 315                 320 


Pro Asp Leu Glu Leu Ile Gly Asp Ile Pro Ser Thr Ile Asn His Ile 
                325                 330                 335     


Glu His Asp Ala Val Lys Val Glu Phe Ala Glu Arg Glu Gln Lys Ile 
            340                 345                 350         


Leu Ser Asp Leu Lys Gln Tyr Met His Glu Gly Glu Gln Val Pro Ala 
        355                 360                 365             


Asp Trp Lys Ser Asp Arg Ala His Pro Leu Glu Ile Val Lys Glu Leu 
    370                 375                 380                 


Arg Asn Ala Val Asp Asp His Val Thr Val Thr Cys Asp Ile Gly Ser 
385                 390                 395                 400 


His Ala Ile Trp Met Ser Arg Tyr Phe Arg Ser Tyr Glu Pro Leu Thr 
                405                 410                 415     


Leu Met Ile Ser Asn Gly Met Gln Thr Leu Gly Val Ala Leu Pro Trp 
            420                 425                 430         


Ala Ile Gly Ala Ser Leu Val Lys Pro Gly Glu Lys Val Val Ser Val 
        435                 440                 445             


Ser Gly Asp Gly Gly Phe Leu Phe Ser Ala Met Glu Leu Glu Thr Ala 
    450                 455                 460                 


Val Arg Leu Lys Ala Pro Ile Val His Ile Val Trp Asn Asp Ser Thr 
465                 470                 475                 480 


Tyr Asp Met Val Ala Phe Gln Gln Leu Lys Lys Tyr Asn Arg Thr Ser 
                485                 490                 495     


Ala Val Asp Phe Gly Asn Ile Asp Ile Val Lys Tyr Ala Glu Ser Phe 
            500                 505                 510         


Gly Ala Thr Gly Leu Arg Val Glu Ser Pro Asp Gln Leu Ala Asp Val 
        515                 520                 525             


Leu Arg Gln Gly Met Asn Ala Glu Gly Pro Val Ile Ile Asp Val Pro 
    530                 535                 540                 


Val Asp Tyr Ser Asp Asn Ile Asn Leu Ala Ser Asp Lys Leu Pro Lys 
545                 550                 555                 560 


Glu Phe Gly Glu Leu Met Lys Thr Lys Ala Leu Leu 
                565                 570         


<210>  13
<211>  1062
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ketolacid reductoisomerase (EC 1.1.1.86), Meiothermus ruber 
       DNA-sequence including C-terminal His-Tag

<400>  13
atgaagattt actacgacca ggacgcagac atcggcttta tcaaagacaa gactgtggcc       60

attctgggct ttggctcgca gggccatgcc cacgccctta acctgcggga ctccggcatc      120

aaggtggtgg tggggctgcg ccccggcagc cgcaacgagg agaaggcccg taaagcgggg      180

ctcgaggtgc ttccggtagg ggaggcggtg cgcagggccg atgtggtgat gatcctgctc      240

ccggacgaga cccagggggc cgtttacaag gccgaggtgg aacccaacct gaaggaaggg      300

gctgcccttg ccttcgccca cggcttcaac atccatttcg gccagatcaa gccgcgccgc      360

gacctggacg tctggatggt ggcccccaaa ggccccggcc acctggtgcg ctcggagtac      420

gagaaaggct cgggcgtgcc ctcgctggtg gcggtctacc aggacgcctc cgggtcggcc      480

ttccccacgg cgctggccta cgccaaggcc aacggcggca cccgcgccgg caccatcgcc      540

accaccttca aggacgagac cgagaccgac ctgttcggcg agcagaccgt gctgtgcggg      600

ggcctgaccc agctcatcgc cgccggtttc gagaccctgg tggaggccgg ctatcccccc      660

gagatggcct actttgagtg cctgcacgag gtgaagctga tcgtggacct gatctacgag      720

tcaggcttcg ccgggatgcg ctactccatc tccaacaccg ccgagtacgg cgactacacc      780

cgcggcccca tggtgatcaa ccgcgaggag accaaggccc gcatgcgcga ggtgctgcgc      840

cagattcagc agggcgagtt tgcccgcgag tggatgctgg aaaacgtggt gggccagccc      900

accctgaacg ccaaccgcaa ctactggaaa gaccacccca tcgagcaggt gggccccaag      960

ctgcgggcca tgatgccctt cctcaagtcc aggttcacga aggaagaggt cggtagcagc     1020

gggagacctg tgctgggcag cagccaccac caccaccacc ac                        1062


<210>  14
<211>  354
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ketolacid reductoisomerase (EC 1.1.1.86), Meiothermus ruber 
       Protein sequence including C-terminal His-Tag

<400>  14

Met Lys Ile Tyr Tyr Asp Gln Asp Ala Asp Ile Gly Phe Ile Lys Asp 
1               5                   10                  15      


Lys Thr Val Ala Ile Leu Gly Phe Gly Ser Gln Gly His Ala His Ala 
            20                  25                  30          


Leu Asn Leu Arg Asp Ser Gly Ile Lys Val Val Val Gly Leu Arg Pro 
        35                  40                  45              


Gly Ser Arg Asn Glu Glu Lys Ala Arg Lys Ala Gly Leu Glu Val Leu 
    50                  55                  60                  


Pro Val Gly Glu Ala Val Arg Arg Ala Asp Val Val Met Ile Leu Leu 
65                  70                  75                  80  


Pro Asp Glu Thr Gln Gly Ala Val Tyr Lys Ala Glu Val Glu Pro Asn 
                85                  90                  95      


Leu Lys Glu Gly Ala Ala Leu Ala Phe Ala His Gly Phe Asn Ile His 
            100                 105                 110         


Phe Gly Gln Ile Lys Pro Arg Arg Asp Leu Asp Val Trp Met Val Ala 
        115                 120                 125             


Pro Lys Gly Pro Gly His Leu Val Arg Ser Glu Tyr Glu Lys Gly Ser 
    130                 135                 140                 


Gly Val Pro Ser Leu Val Ala Val Tyr Gln Asp Ala Ser Gly Ser Ala 
145                 150                 155                 160 


Phe Pro Thr Ala Leu Ala Tyr Ala Lys Ala Asn Gly Gly Thr Arg Ala 
                165                 170                 175     


Gly Thr Ile Ala Thr Thr Phe Lys Asp Glu Thr Glu Thr Asp Leu Phe 
            180                 185                 190         


Gly Glu Gln Thr Val Leu Cys Gly Gly Leu Thr Gln Leu Ile Ala Ala 
        195                 200                 205             


Gly Phe Glu Thr Leu Val Glu Ala Gly Tyr Pro Pro Glu Met Ala Tyr 
    210                 215                 220                 


Phe Glu Cys Leu His Glu Val Lys Leu Ile Val Asp Leu Ile Tyr Glu 
225                 230                 235                 240 


Ser Gly Phe Ala Gly Met Arg Tyr Ser Ile Ser Asn Thr Ala Glu Tyr 
                245                 250                 255     


Gly Asp Tyr Thr Arg Gly Pro Met Val Ile Asn Arg Glu Glu Thr Lys 
            260                 265                 270         


Ala Arg Met Arg Glu Val Leu Arg Gln Ile Gln Gln Gly Glu Phe Ala 
        275                 280                 285             


Arg Glu Trp Met Leu Glu Asn Val Val Gly Gln Pro Thr Leu Asn Ala 
    290                 295                 300                 


Asn Arg Asn Tyr Trp Lys Asp His Pro Ile Glu Gln Val Gly Pro Lys 
305                 310                 315                 320 


Leu Arg Ala Met Met Pro Phe Leu Lys Ser Arg Phe Thr Lys Glu Glu 
                325                 330                 335     


Val Gly Ser Ser Gly Arg Pro Val Leu Gly Ser Ser His His His His 
            340                 345                 350         


His His 
        


<210>  15
<211>  1713
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Branched-chain ketoacid decarboxylase (EC 4.1.1.72), Lactococcus 
       lactis DNA-sequence including N-terminal His-Tag

<400>  15
atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat       60

atggctagca tgtatacagt aggagattac ctgttagacc gattacacga gttgggaatt      120

gaagaaattt ttggagttcc tggtgactat aacttacaat ttttagatca aattatttca      180

cgcgaagata tgaaatggat tggaaatgct aatgaattaa atgcttctta tatggctgat      240

ggttatgctc gtactaaaaa agctgccgca tttctcacca catttggagt cggcgaattg      300

agtgcgatca atggactggc aggaagttat gccgaaaatt taccagtagt agaaattgtt      360

ggttcaccaa cttcaaaagt acaaaatgac ggaaaatttg tccatcatac actagcagat      420

ggtgatttta aacactttat gaagatgcat gaacctgtta cagcagcgcg gactttactg      480

acagcagaaa atgccacata tgaaattgac cgagtacttt ctcaattact aaaagaaaga      540

aaaccagtct atattaactt accagtcgat gttgctgcag caaaagcaga gaagcctgca      600

ttatctttag aaaaagaaag ctctacaaca aatacaactg aacaagtgat tttgagtaag      660

attgaagaaa gtttgaaaaa tgcccaaaaa ccagtagtga ttgcaggaca cgaagtaatt      720

agttttggtt tagaaaaaac ggtaactcag tttgtttcag aaacaaaact accgattacg      780

acactaaatt ttggtaaaag tgctgttgat gaatctttgc cctcattttt aggaatatat      840

aacgggaaac tttcagaaat cagtcttaaa aattttgtgg agtccgcaga ctttatccta      900

atgcttggag tgaagcttac ggactcctca acaggtgcat tcacacatca tttagatgaa      960

aataaaatga tttcactaaa catagatgaa ggaataattt tcaataaagt ggtagaagat     1020

tttgatttta gagcagtggt ttcttcttta tcagaattaa aaggaataga atatgaagga     1080

caatatattg ataagcaata tgaagaattt attccatcaa gtgctccctt atcacaagac     1140

cgtctatggc aggcagttga aagtttgact caaagcaatg aaacaatcgt tgctgaacaa     1200

ggaacctcat tttttggagc ttcaacaatt ttcttaaaat caaatagtcg ttttattgga     1260

caacctttat ggggttctat tggatatact tttccagcgg ctttaggaag ccaaattgcg     1320

gataaagaga gcagacacct tttatttatt ggtgatggtt cacttcaact taccgtacaa     1380

gaattaggac tatcaatcag agaaaaactc aatccaattt gttttatcat aaataatgat     1440

ggttatacag ttgaaagaga aatccacgga cctactcaaa gttataacga cattccaatg     1500

tggaattact cgaaattacc agaaacattt ggagcaacag aagatcgtgt agtatcaaaa     1560

attgttagaa cagagaatga atttgtgtct gtcatgaaag aagcccaagc agatgtcaat     1620

agaatgtatt ggatagaact agttttggaa aaagaagatg cgccaaaatt actgaaaaaa     1680

atgggtaaat tatttgctga gcaaaataaa taa                                  1713


<210>  16
<211>  570
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Branched-chain ketoacid decarboxylase (EC 4.1.1.72), Lactococcus 
       lactis Protein sequence including N-terminal His-Tag

<400>  16

Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His Met Ala Ser Met Tyr Thr Val Gly Asp Tyr Leu Leu 
            20                  25                  30          


Asp Arg Leu His Glu Leu Gly Ile Glu Glu Ile Phe Gly Val Pro Gly 
        35                  40                  45              


Asp Tyr Asn Leu Gln Phe Leu Asp Gln Ile Ile Ser Arg Glu Asp Met 
    50                  55                  60                  


Lys Trp Ile Gly Asn Ala Asn Glu Leu Asn Ala Ser Tyr Met Ala Asp 
65                  70                  75                  80  


Gly Tyr Ala Arg Thr Lys Lys Ala Ala Ala Phe Leu Thr Thr Phe Gly 
                85                  90                  95      


Val Gly Glu Leu Ser Ala Ile Asn Gly Leu Ala Gly Ser Tyr Ala Glu 
            100                 105                 110         


Asn Leu Pro Val Val Glu Ile Val Gly Ser Pro Thr Ser Lys Val Gln 
        115                 120                 125             


Asn Asp Gly Lys Phe Val His His Thr Leu Ala Asp Gly Asp Phe Lys 
    130                 135                 140                 


His Phe Met Lys Met His Glu Pro Val Thr Ala Ala Arg Thr Leu Leu 
145                 150                 155                 160 


Thr Ala Glu Asn Ala Thr Tyr Glu Ile Asp Arg Val Leu Ser Gln Leu 
                165                 170                 175     


Leu Lys Glu Arg Lys Pro Val Tyr Ile Asn Leu Pro Val Asp Val Ala 
            180                 185                 190         


Ala Ala Lys Ala Glu Lys Pro Ala Leu Ser Leu Glu Lys Glu Ser Ser 
        195                 200                 205             


Thr Thr Asn Thr Thr Glu Gln Val Ile Leu Ser Lys Ile Glu Glu Ser 
    210                 215                 220                 


Leu Lys Asn Ala Gln Lys Pro Val Val Ile Ala Gly His Glu Val Ile 
225                 230                 235                 240 


Ser Phe Gly Leu Glu Lys Thr Val Thr Gln Phe Val Ser Glu Thr Lys 
                245                 250                 255     


Leu Pro Ile Thr Thr Leu Asn Phe Gly Lys Ser Ala Val Asp Glu Ser 
            260                 265                 270         


Leu Pro Ser Phe Leu Gly Ile Tyr Asn Gly Lys Leu Ser Glu Ile Ser 
        275                 280                 285             


Leu Lys Asn Phe Val Glu Ser Ala Asp Phe Ile Leu Met Leu Gly Val 
    290                 295                 300                 


Lys Leu Thr Asp Ser Ser Thr Gly Ala Phe Thr His His Leu Asp Glu 
305                 310                 315                 320 


Asn Lys Met Ile Ser Leu Asn Ile Asp Glu Gly Ile Ile Phe Asn Lys 
                325                 330                 335     


Val Val Glu Asp Phe Asp Phe Arg Ala Val Val Ser Ser Leu Ser Glu 
            340                 345                 350         


Leu Lys Gly Ile Glu Tyr Glu Gly Gln Tyr Ile Asp Lys Gln Tyr Glu 
        355                 360                 365             


Glu Phe Ile Pro Ser Ser Ala Pro Leu Ser Gln Asp Arg Leu Trp Gln 
    370                 375                 380                 


Ala Val Glu Ser Leu Thr Gln Ser Asn Glu Thr Ile Val Ala Glu Gln 
385                 390                 395                 400 


Gly Thr Ser Phe Phe Gly Ala Ser Thr Ile Phe Leu Lys Ser Asn Ser 
                405                 410                 415     


Arg Phe Ile Gly Gln Pro Leu Trp Gly Ser Ile Gly Tyr Thr Phe Pro 
            420                 425                 430         


Ala Ala Leu Gly Ser Gln Ile Ala Asp Lys Glu Ser Arg His Leu Leu 
        435                 440                 445             


Phe Ile Gly Asp Gly Ser Leu Gln Leu Thr Val Gln Glu Leu Gly Leu 
    450                 455                 460                 


Ser Ile Arg Glu Lys Leu Asn Pro Ile Cys Phe Ile Ile Asn Asn Asp 
465                 470                 475                 480 


Gly Tyr Thr Val Glu Arg Glu Ile His Gly Pro Thr Gln Ser Tyr Asn 
                485                 490                 495     


Asp Ile Pro Met Trp Asn Tyr Ser Lys Leu Pro Glu Thr Phe Gly Ala 
            500                 505                 510         


Thr Glu Asp Arg Val Val Ser Lys Ile Val Arg Thr Glu Asn Glu Phe 
        515                 520                 525             


Val Ser Val Met Lys Glu Ala Gln Ala Asp Val Asn Arg Met Tyr Trp 
    530                 535                 540                 


Ile Glu Leu Val Leu Glu Lys Glu Asp Ala Pro Lys Leu Leu Lys Lys 
545                 550                 555                 560 


Met Gly Lys Leu Phe Ala Glu Gln Asn Lys 
                565                 570 


<210>  17
<211>  1044
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Alcohol dehydrogenase (EC 1.1.1.1), Geobacillus 
       stearothermophilus DNA-sequence including C-terminal His-Tag

<400>  17
atgaaagcag cagttgtgga acagtttaaa gaaccgctga aaatcaaaga ggtggaaaaa       60

ccgaccatta gctatggtga agttctggtt cgtattaaag cctgtggtgt ttgtcatacc      120

gatctgcatg cagcacatgg tgattggcct gttaaaccga aactgccgct gattccgggt      180

catgaaggtg ttggtattgt tgaagaggtt ggtccgggtg ttacccatct gaaagttggt      240

gatcgtgttg gtattccgtg gctgtatagc gcatgtggtc attgtgatta ttgtctgagc      300

ggtcaagaaa ccctgtgtga acatcagaaa aatgcaggtt atagcgtgga tggtggttat      360

gcagaatatt gtcgtgcagc agcagattat gttgtgaaaa ttccggataa cctgagcttt      420

gaagaagcag caccgatttt ttgtgccggt gttaccacct ataaagcact gaaagttacc      480

ggtgcaaaac cgggtgaatg ggttgcaatt tatggtattg gtggtctggg ccatgttgca      540

gttcagtatg caaaagcaat gggtctgaat gttgttgcag tggatatcgg tgatgaaaaa      600

ctggaactgg caaaagaact gggtgcagat ctggttgtta atccgctgaa agaagatgca      660

gccaaattta tgaaagaaaa agtgggtggt gttcatgcag cagttgttac cgcagttagc      720

aaaccggcat ttcagagcgc atataatagc attcgtcgtg gtggtgcatg tgttctggtt      780

ggtctgcctc cggaagaaat gccgattccg atttttgata ccgttctgaa cggcattaaa      840

atcattggta gcattgttgg cacccgtaaa gatctgcaag aagcactgca gtttgcagca      900

gaaggtaaag ttaaaaccat cattgaagtt cagccgctgg aaaaaatcaa cgaagttttt      960

gatcgcatgc tgaaaggtca gattaatggt cgtgttgttc tgaccctgga agataaactc     1020

gagcaccacc accaccacca ctga                                            1044


<210>  18
<211>  347
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Alcohol dehydrogenase (EC 1.1.1.1), Geobacillus 
       stearothermophilus Protein sequence including C-terminal His-Tag

<400>  18

Met Lys Ala Ala Val Val Glu Gln Phe Lys Glu Pro Leu Lys Ile Lys 
1               5                   10                  15      


Glu Val Glu Lys Pro Thr Ile Ser Tyr Gly Glu Val Leu Val Arg Ile 
            20                  25                  30          


Lys Ala Cys Gly Val Cys His Thr Asp Leu His Ala Ala His Gly Asp 
        35                  40                  45              


Trp Pro Val Lys Pro Lys Leu Pro Leu Ile Pro Gly His Glu Gly Val 
    50                  55                  60                  


Gly Ile Val Glu Glu Val Gly Pro Gly Val Thr His Leu Lys Val Gly 
65                  70                  75                  80  


Asp Arg Val Gly Ile Pro Trp Leu Tyr Ser Ala Cys Gly His Cys Asp 
                85                  90                  95      


Tyr Cys Leu Ser Gly Gln Glu Thr Leu Cys Glu His Gln Lys Asn Ala 
            100                 105                 110         


Gly Tyr Ser Val Asp Gly Gly Tyr Ala Glu Tyr Cys Arg Ala Ala Ala 
        115                 120                 125             


Asp Tyr Val Val Lys Ile Pro Asp Asn Leu Ser Phe Glu Glu Ala Ala 
    130                 135                 140                 


Pro Ile Phe Cys Ala Gly Val Thr Thr Tyr Lys Ala Leu Lys Val Thr 
145                 150                 155                 160 


Gly Ala Lys Pro Gly Glu Trp Val Ala Ile Tyr Gly Ile Gly Gly Leu 
                165                 170                 175     


Gly His Val Ala Val Gln Tyr Ala Lys Ala Met Gly Leu Asn Val Val 
            180                 185                 190         


Ala Val Asp Ile Gly Asp Glu Lys Leu Glu Leu Ala Lys Glu Leu Gly 
        195                 200                 205             


Ala Asp Leu Val Val Asn Pro Leu Lys Glu Asp Ala Ala Lys Phe Met 
    210                 215                 220                 


Lys Glu Lys Val Gly Gly Val His Ala Ala Val Val Thr Ala Val Ser 
225                 230                 235                 240 


Lys Pro Ala Phe Gln Ser Ala Tyr Asn Ser Ile Arg Arg Gly Gly Ala 
                245                 250                 255     


Cys Val Leu Val Gly Leu Pro Pro Glu Glu Met Pro Ile Pro Ile Phe 
            260                 265                 270         


Asp Thr Val Leu Asn Gly Ile Lys Ile Ile Gly Ser Ile Val Gly Thr 
        275                 280                 285             


Arg Lys Asp Leu Gln Glu Ala Leu Gln Phe Ala Ala Glu Gly Lys Val 
    290                 295                 300                 


Lys Thr Ile Ile Glu Val Gln Pro Leu Glu Lys Ile Asn Glu Val Phe 
305                 310                 315                 320 


Asp Arg Met Leu Lys Gly Gln Ile Asn Gly Arg Val Val Leu Thr Leu 
                325                 330                 335     


Glu Asp Lys Leu Glu His His His His His His 
            340                 345         


<210>  19
<211>  1752
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Pyruvate decarboxylase (EC 4.1.1.1), Zymomonas mobilis 
       DNA-sequence including C-terminal His-Tag

<400>  19
atgagctata ccgttggcac ctatctggca gaacgtctgg ttcagattgg tctgaaacat       60

cattttgcag tggcaggcga ttataatctg gtgctgctgg ataatctgct gctgaataaa      120

aatatggaac aggtgtattg ctgcaatgaa ctgaattgtg gctttagcgc tgaaggttat      180

gcacgtgcaa aaggtgcagc agcagcagtt gttacctata gcgttggtgc actgagcgca      240

tttgatgcca ttggtggtgc ttatgcagaa aatctgccgg ttattctgat ttctggtgca      300

ccgaataata atgatcatgc cgcaggccat gttctgcatc atgcactggg taaaaccgat      360

tatcattatc agctggaaat ggccaaaaat attaccgcag cagccgaagc aatttataca      420

ccggaagaag caccggcaaa aattgatcat gtgattaaaa ccgcactgcg tgaaaaaaaa      480

ccggtgtatc tggaaattgc ctgtaatatt gcaagcatgc cgtgtgcagc accgggtccg      540

gcaagcgcac tgtttaatga tgaagcctct gatgaagcaa gcctgaatgc agcagttgaa      600

gaaaccctga aatttattgc caatcgcgat aaagttgcag ttctggttgg tagcaaactg      660

cgtgcagccg gtgcagaaga agcagcagtt aaatttgcag atgcactggg tggtgcagtt      720

gcaaccatgg cagcagcaaa aagttttttt ccggaagaaa atccgcatta cattggcacc      780

agctggggtg aagttagcta tccgggtgtt gaaaaaacca tgaaagaagc cgacgcagtt      840

attgcactgg caccggtgtt taatgattat agcaccaccg gttggaccga tattccggat      900

ccgaaaaaac tggttctggc cgaaccgcgt agcgttgttg ttaatggtat tcgttttccg      960

agcgtgcatc tgaaagatta tctgacccgt ctggcacaga aagttagcaa aaaaacaggt     1020

gccctggatt tttttaaatc cctgaatgcc ggtgaactga aaaaagcagc accggcagat     1080

ccgagcgcac cgctggttaa tgcagaaatt gcacgtcagg ttgaagcact gctgaccccg     1140

aataccaccg ttattgcaga aaccggtgat agctggttta atgcccagcg tatgaaactg     1200

ccgaatggtg cacgtgttga atatgaaatg cagtggggtc atattggttg gagcgttccg     1260

gcagcatttg gttatgcagt tggtgcaccg gaacgtcgta atattctgat ggttggtgat     1320

ggtagctttc agctgaccgc acaagaggtt gcacagatgg ttcgtctgaa actgccggtg     1380

attatttttc tgattaataa ttatggctat accattgaag tgatgattca tgatggtccg     1440

tataataata ttaaaaattg ggattatgcc ggtctgatgg aagtgtttaa tggcaatggt     1500

ggttatgata gcggtgccgg taaaggtctg aaagcaaaaa ccggtggtga actggcagaa     1560

gcaattaaag ttgcactggc caataccgat ggtccgaccc tgattgaatg ttttattggt     1620

cgcgaagatt gtaccgaaga actggtgaaa tggggtaaac gtgttgcagc agcaaatagc     1680

cgtaaaccgg tgaataaact gctgagcggg agacctgtgc tgggcagcag ccaccaccac     1740

caccaccact aa                                                         1752


<210>  20
<211>  583
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Pyruvate decarboxylase (EC 4.1.1.1), Zymomonas mobilis Protein 
       sequence including C-terminal His-Tag

<400>  20

Met Ser Tyr Thr Val Gly Thr Tyr Leu Ala Glu Arg Leu Val Gln Ile 
1               5                   10                  15      


Gly Leu Lys His His Phe Ala Val Ala Gly Asp Tyr Asn Leu Val Leu 
            20                  25                  30          


Leu Asp Asn Leu Leu Leu Asn Lys Asn Met Glu Gln Val Tyr Cys Cys 
        35                  40                  45              


Asn Glu Leu Asn Cys Gly Phe Ser Ala Glu Gly Tyr Ala Arg Ala Lys 
    50                  55                  60                  


Gly Ala Ala Ala Ala Val Val Thr Tyr Ser Val Gly Ala Leu Ser Ala 
65                  70                  75                  80  


Phe Asp Ala Ile Gly Gly Ala Tyr Ala Glu Asn Leu Pro Val Ile Leu 
                85                  90                  95      


Ile Ser Gly Ala Pro Asn Asn Asn Asp His Ala Ala Gly His Val Leu 
            100                 105                 110         


His His Ala Leu Gly Lys Thr Asp Tyr His Tyr Gln Leu Glu Met Ala 
        115                 120                 125             


Lys Asn Ile Thr Ala Ala Ala Glu Ala Ile Tyr Thr Pro Glu Glu Ala 
    130                 135                 140                 


Pro Ala Lys Ile Asp His Val Ile Lys Thr Ala Leu Arg Glu Lys Lys 
145                 150                 155                 160 


Pro Val Tyr Leu Glu Ile Ala Cys Asn Ile Ala Ser Met Pro Cys Ala 
                165                 170                 175     


Ala Pro Gly Pro Ala Ser Ala Leu Phe Asn Asp Glu Ala Ser Asp Glu 
            180                 185                 190         


Ala Ser Leu Asn Ala Ala Val Glu Glu Thr Leu Lys Phe Ile Ala Asn 
        195                 200                 205             


Arg Asp Lys Val Ala Val Leu Val Gly Ser Lys Leu Arg Ala Ala Gly 
    210                 215                 220                 


Ala Glu Glu Ala Ala Val Lys Phe Ala Asp Ala Leu Gly Gly Ala Val 
225                 230                 235                 240 


Ala Thr Met Ala Ala Ala Lys Ser Phe Phe Pro Glu Glu Asn Pro His 
                245                 250                 255     


Tyr Ile Gly Thr Ser Trp Gly Glu Val Ser Tyr Pro Gly Val Glu Lys 
            260                 265                 270         


Thr Met Lys Glu Ala Asp Ala Val Ile Ala Leu Ala Pro Val Phe Asn 
        275                 280                 285             


Asp Tyr Ser Thr Thr Gly Trp Thr Asp Ile Pro Asp Pro Lys Lys Leu 
    290                 295                 300                 


Val Leu Ala Glu Pro Arg Ser Val Val Val Asn Gly Ile Arg Phe Pro 
305                 310                 315                 320 


Ser Val His Leu Lys Asp Tyr Leu Thr Arg Leu Ala Gln Lys Val Ser 
                325                 330                 335     


Lys Lys Thr Gly Ala Leu Asp Phe Phe Lys Ser Leu Asn Ala Gly Glu 
            340                 345                 350         


Leu Lys Lys Ala Ala Pro Ala Asp Pro Ser Ala Pro Leu Val Asn Ala 
        355                 360                 365             


Glu Ile Ala Arg Gln Val Glu Ala Leu Leu Thr Pro Asn Thr Thr Val 
    370                 375                 380                 


Ile Ala Glu Thr Gly Asp Ser Trp Phe Asn Ala Gln Arg Met Lys Leu 
385                 390                 395                 400 


Pro Asn Gly Ala Arg Val Glu Tyr Glu Met Gln Trp Gly His Ile Gly 
                405                 410                 415     


Trp Ser Val Pro Ala Ala Phe Gly Tyr Ala Val Gly Ala Pro Glu Arg 
            420                 425                 430         


Arg Asn Ile Leu Met Val Gly Asp Gly Ser Phe Gln Leu Thr Ala Gln 
        435                 440                 445             


Glu Val Ala Gln Met Val Arg Leu Lys Leu Pro Val Ile Ile Phe Leu 
    450                 455                 460                 


Ile Asn Asn Tyr Gly Tyr Thr Ile Glu Val Met Ile His Asp Gly Pro 
465                 470                 475                 480 


Tyr Asn Asn Ile Lys Asn Trp Asp Tyr Ala Gly Leu Met Glu Val Phe 
                485                 490                 495     


Asn Gly Asn Gly Gly Tyr Asp Ser Gly Ala Gly Lys Gly Leu Lys Ala 
            500                 505                 510         


Lys Thr Gly Gly Glu Leu Ala Glu Ala Ile Lys Val Ala Leu Ala Asn 
        515                 520                 525             


Thr Asp Gly Pro Thr Leu Ile Glu Cys Phe Ile Gly Arg Glu Asp Cys 
    530                 535                 540                 


Thr Glu Glu Leu Val Lys Trp Gly Lys Arg Val Ala Ala Ala Asn Ser 
545                 550                 555                 560 


Arg Lys Pro Val Asn Lys Leu Leu Ser Gly Arg Pro Val Leu Gly Ser 
                565                 570                 575     


Ser His His His His His His 
            580             


<210>  21
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence Fw-Mut (65C)

<400>  21
gaattgtgag cggataacaa ttccc                                             25


<210>  22
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence Rev-Mut (65C)

<400>  22
ctttgttagc agccggatct c                                                 21


<210>  23
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence Fw-F34 (71C)


<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  n is a, c, g, or t

<400>  23
cggtcaggtt attggtcgtn nkgaagcagc aacccgtg                               38


<210>  24
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence Rev-F34 (71C)


<220>
<221>  misc_feature
<222>  (18)..(19)
<223>  n is a, c, g, or t

<400>  24
cacgggttgc tgcttcmnna cgaccaataa cctgaccg                               38


<210>  25
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence Fw-S405 (64C)


<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  n is a, c, g, or t

<400>  25
gtatgatctg gccaatgatn nkaaatatgg tctggccag                              39


<210>  26
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence Rev-S405 (64C)


<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<400>  26
ctggccagac catatttmnn atcattggcc agatcatac                              39


<210>  27
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence Fw-W271 (60C)


<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  n is a, c, g, or t

<400>  27
gaaaaccctg ctgnnkgcaa aatattggaa tg                                     32


<210>  28
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence Rev-W271 (60C)


<220>
<221>  misc_feature
<222>  (18)..(19)
<223>  n is a, c, g, or t

<400>  28
cattccaata ttttgcmnnc agcagggttt tc                                     32


<210>  29
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence Fw-Y399 (59C)


<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  n is a, c, g, or t

<400>  29
cgtggaagaa atgnnkgatc tggccaat                                          28


<210>  30
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence Rev-Y399 (59C)


<220>
<221>  misc_feature
<222>  (14)..(15)
<223>  n is a, c, g, or t

<400>  30
attggccaga tcmnncattt cttccacg                                          28


<210>  31
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence Fw-F34L (75C)

<400>  31
ggtcaggtta ttggtcgttt agaagcagca acccgtg                                37


<210>  32
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence Rev-F34L (75C)

<400>  32
cacgggttgc tgcttctaaa cgaccaataa cctgacc                                37


<210>  33
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence Fw-F34M (68C)

<400>  33
gtcaggttat tggtcgtatg gaagcagcaa cccgt                                  35


<210>  34
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence Rev-F34M (68C)

<400>  34
acgggttgct gcttccatac gaccaataac ctgac                                  35


<210>  35
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence Fw-W271S (65C)

<400>  35
gaaaaccctg ctgtcggcaa aatattggaa tg                                     32


<210>  36
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence Rev-W271S (65C)

<400>  36
cattccaata ttttgccgac agcagggttt tc                                     32


<210>  37
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence Fw-Y399C (63C)

<400>  37
cgtggaagaa atgtgtgatc tggccaatg                                         29


<210>  38
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence Rev-Y399C (63C)

<400>  38
cattggccag atcacacatt tcttccacg                                         29


<210>  39
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence Fw-S405C (73C)

<400>  39
gtatgatctg gccaatgatt gcaaatatgg tctggcc                                37


<210>  40
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence Rev-S405C (73C)

<400>  40
ggccagacca tatttgcaat cattggccag atcatac                                37


<210>  41
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence Fw-S405N (68C)

<400>  41
tgatctggcc aatgataaca aatatggtct ggcca                                  35


<210>  42
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence Rev-S405N (68C)

<400>  42
tggccagacc atatttgtta tcattggcca gatca                                  35


<210>  43
<211>  105
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCBR multiple cloning site

<400>  43
atatatatat tctagaaata attttgttta actttaagaa ggagatatac atatgatgca       60

ggtatatata tattaataga gacctcctcg gatccatata tatat                      105


<210>  44
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCBRHisN multiple cloning site

<400>  44
atatatatat catatgatgc aggtatatat atattaatag agacctcctc gaattcatat       60

atatat                                                                  66


<210>  45
<211>  192
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCBRHisC multiple cloning site

<400>  45
atatatatat tctagaaata attttgttta actttaagaa ggagatatac atatgatgca       60

ggtatatata tatagcggga gacctgtgct gggcagcagc caccaccacc accaccacta      120

atgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga      180

gcatatatat at                                                          192


<210>  46
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence SsGDH_for

<400>  46
cagcaaggtc tcacatatga aagccattat tgtgaaacct ccg                         43


<210>  47
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence SsGDH_rev

<400>  47
ttcccacaga atacgaattt tgatttcgc                                         29


<210>  48
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence SsDHAD_for

<400>  48
cagcaaggtc tcacatatgc ctgcaaaact gaatagccc                              39


<210>  49
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence SsDHAD_rev

<400>  49
tgccggacgg gtaactgc                                                     18


<210>  50
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence SaKDGA_for

<400>  50
cagcaaggtc tcacatatgg aaattattag cccgattatt accc                        44


<210>  51
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence SaKDGA_rev

<400>  51
atgaaccagt tcctgaattt tgcg                                              24


<210>  52
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence TaALDH_for

<400>  52
cagcaaggtc tcacatatgg ataccaaact gtatattgat ggc                         43


<210>  53
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence TaALDH_rev

<400>  53
ctgaaacagg tcatcacgaa cg                                                22


<210>  54
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence MrKARI_for

<400>  54
cagcaacgtc tcgcatatga agatttacta cgaccaggac gcag                        44


<210>  55
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer sequence MrKARI_rev

<400>  55
gctaccgacc tcttccttcg tgaac                                             25


<210>  56
<211>  1527
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  taALDH-F34L-Chis

<400>  56
atggatacca aactgtatat tgatggccag tgggttaata gcagcagcgg taaaaccgtt       60

gataaatatt ctccggttac cggtcaggtt attggtcgtt tagaagcagc aacccgtgat      120

gatgttgatc gtgcaattga tgcagcagaa gatgcatttt gggcctggaa tgatctgggt      180

agcgttgaac gcagcaaaat tatttatcgt gccaaagaac tgattgaaaa aaatcgtgcc      240

gaactggaaa atattattat ggaagaaaat ggcaaaccgg tgaaagaagc caaagaagaa      300

gtcgacggcg tcattgatca gattcagtat tatgcagaat gggcacgtaa actgaatggt      360

gaagttgttg aaggcaccag cagccatcgt aaaatttttc agtataaagt gccgtatggt      420

attgttgttg cactgacccc gtggaatttt ccggcaggca tggttgcccg taaactggca      480

ccggcactgc tgaccggtaa taccgttgtt ctgaaaccga gcagcgatac accgggtagc      540

gcagaatgga ttgtgcgtaa atttgttgaa gccggtgttc cgaaaggtgt gctgaatttt      600

attaccggtc gtggtagcga aattggcgat tacattgtgg aacataaaaa agtcaatctg      660

attaccatga ccggtagcac cgcaacaggt cagcgcatta tgcagaaagc aagcgcaaat      720

atggcaaaac tgattctgga actgggtggt aaagcaccgt ttatggtttg gaaagatgcc      780

gatatggata atgcactgaa aaccctgctg tgggcaaaat attggaatgc cggtcagagc      840

tgtattgcag cagaacgtct gtatgtgcat gaagatattt atgatacctt tatgagccgt      900

tttgttgaac tgagccgcaa actggcactg ggtgatccga aaaatgcaga tatgggtccg      960

ctgattaata aaggtgcact gcaggcaacc agcgaaattg ttgaagaagc gaaagaatct     1020

ggcgcaaaaa ttctgtttgg tggtagccag ccgagcctga gcggtccgta tcgtaatggc     1080

tatttttttc tgccgaccat tattggtaat gcggatcaga aaagcaaaat ctttcaggaa     1140

gaaatttttg caccggttat tggtgcacgt aaaattagca gcgtggaaga aatgtatgat     1200

ctggccaatg atagcaaata tggtctggcc agctacctgt ttaccaaaga tccgaatatc     1260

atttttgaag ccagcgaacg tattcgtttt ggtgaactgt atgtgaatat gccgggtccg     1320

gaagcaagcc agggttatca caccggtttt cgtatgacag gtcaggcagg cgaaggttct     1380

aaatatggca ttagcgaata tctgaaactg aaaaatattt atgtggatta tagcggcaaa     1440

ccgctgcata ttaataccgt tcgtgatgac ctgtttcaga gcgggagacc tgtgctgggc     1500

agcagccacc accaccacca ccactaa                                         1527


<210>  57
<211>  508
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  taALDH-F34L-Chis

<400>  57

Met Asp Thr Lys Leu Tyr Ile Asp Gly Gln Trp Val Asn Ser Ser Ser 
1               5                   10                  15      


Gly Lys Thr Val Asp Lys Tyr Ser Pro Val Thr Gly Gln Val Ile Gly 
            20                  25                  30          


Arg Leu Glu Ala Ala Thr Arg Asp Asp Val Asp Arg Ala Ile Asp Ala 
        35                  40                  45              


Ala Glu Asp Ala Phe Trp Ala Trp Asn Asp Leu Gly Ser Val Glu Arg 
    50                  55                  60                  


Ser Lys Ile Ile Tyr Arg Ala Lys Glu Leu Ile Glu Lys Asn Arg Ala 
65                  70                  75                  80  


Glu Leu Glu Asn Ile Ile Met Glu Glu Asn Gly Lys Pro Val Lys Glu 
                85                  90                  95      


Ala Lys Glu Glu Val Asp Gly Val Ile Asp Gln Ile Gln Tyr Tyr Ala 
            100                 105                 110         


Glu Trp Ala Arg Lys Leu Asn Gly Glu Val Val Glu Gly Thr Ser Ser 
        115                 120                 125             


His Arg Lys Ile Phe Gln Tyr Lys Val Pro Tyr Gly Ile Val Val Ala 
    130                 135                 140                 


Leu Thr Pro Trp Asn Phe Pro Ala Gly Met Val Ala Arg Lys Leu Ala 
145                 150                 155                 160 


Pro Ala Leu Leu Thr Gly Asn Thr Val Val Leu Lys Pro Ser Ser Asp 
                165                 170                 175     


Thr Pro Gly Ser Ala Glu Trp Ile Val Arg Lys Phe Val Glu Ala Gly 
            180                 185                 190         


Val Pro Lys Gly Val Leu Asn Phe Ile Thr Gly Arg Gly Ser Glu Ile 
        195                 200                 205             


Gly Asp Tyr Ile Val Glu His Lys Lys Val Asn Leu Ile Thr Met Thr 
    210                 215                 220                 


Gly Ser Thr Ala Thr Gly Gln Arg Ile Met Gln Lys Ala Ser Ala Asn 
225                 230                 235                 240 


Met Ala Lys Leu Ile Leu Glu Leu Gly Gly Lys Ala Pro Phe Met Val 
                245                 250                 255     


Trp Lys Asp Ala Asp Met Asp Asn Ala Leu Lys Thr Leu Leu Trp Ala 
            260                 265                 270         


Lys Tyr Trp Asn Ala Gly Gln Ser Cys Ile Ala Ala Glu Arg Leu Tyr 
        275                 280                 285             


Val His Glu Asp Ile Tyr Asp Thr Phe Met Ser Arg Phe Val Glu Leu 
    290                 295                 300                 


Ser Arg Lys Leu Ala Leu Gly Asp Pro Lys Asn Ala Asp Met Gly Pro 
305                 310                 315                 320 


Leu Ile Asn Lys Gly Ala Leu Gln Ala Thr Ser Glu Ile Val Glu Glu 
                325                 330                 335     


Ala Lys Glu Ser Gly Ala Lys Ile Leu Phe Gly Gly Ser Gln Pro Ser 
            340                 345                 350         


Leu Ser Gly Pro Tyr Arg Asn Gly Tyr Phe Phe Leu Pro Thr Ile Ile 
        355                 360                 365             


Gly Asn Ala Asp Gln Lys Ser Lys Ile Phe Gln Glu Glu Ile Phe Ala 
    370                 375                 380                 


Pro Val Ile Gly Ala Arg Lys Ile Ser Ser Val Glu Glu Met Tyr Asp 
385                 390                 395                 400 


Leu Ala Asn Asp Ser Lys Tyr Gly Leu Ala Ser Tyr Leu Phe Thr Lys 
                405                 410                 415     


Asp Pro Asn Ile Ile Phe Glu Ala Ser Glu Arg Ile Arg Phe Gly Glu 
            420                 425                 430         


Leu Tyr Val Asn Met Pro Gly Pro Glu Ala Ser Gln Gly Tyr His Thr 
        435                 440                 445             


Gly Phe Arg Met Thr Gly Gln Ala Gly Glu Gly Ser Lys Tyr Gly Ile 
    450                 455                 460                 


Ser Glu Tyr Leu Lys Leu Lys Asn Ile Tyr Val Asp Tyr Ser Gly Lys 
465                 470                 475                 480 


Pro Leu His Ile Asn Thr Val Arg Asp Asp Leu Phe Gln Ser Gly Arg 
                485                 490                 495     


Pro Val Leu Gly Ser Ser His His His His His His 
            500                 505             


<210>  58
<211>  1527
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TaALDH-S405C-Chis

<400>  58
atggatacca aactgtatat tgatggccag tgggttaata gcagcagcgg taaaaccgtt       60

gataaatatt ctccggttac cggtcaggtt attggtcgtt ttgaagcagc aacccgtgat      120

gatgttgatc gtgcaattga tgcagcagaa gatgcatttt gggcctggaa tgatctgggt      180

agcgttgaac gcagcaaaat tatttatcgt gccaaagaac tgattgaaaa aaatcgtgcc      240

gaactggaaa atattattat ggaagaaaat ggcaaaccgg tgaaagaagc caaagaagaa      300

gtcgacggcg tcattgatca gattcagtat tatgcagaat gggcacgtaa actgaatggt      360

gaagttgttg aaggcaccag cagccatcgt aaaatttttc agtataaagt gccgtatggt      420

attgttgttg cactgacccc gtggaatttt ccggcaggca tggttgcccg taaactggca      480

ccggcactgc tgaccggtaa taccgttgtt ctgaaaccga gcagcgatac accgggtagc      540

gcagaatgga ttgtgcgtaa atttgttgaa gccggtgttc cgaaaggtgt gctgaatttt      600

attaccggtc gtggtagcga aattggcgat tacattgtgg aacataaaaa agtcaatctg      660

attaccatga ccggtagcac cgcaacaggt cagcgcatta tgcagaaagc aagcgcaaat      720

atggcaaaac tgattctgga actgggtggt aaagcaccgt ttatggtttg gaaagatgcc      780

gatatggata atgcactgaa aaccctgctg tgggcaaaat attggaatgc cggtcagagc      840

tgtattgcag cagaacgtct gtatgtgcat gaagatattt atgatacctt tatgagccgt      900

tttgttgaac tgagccgcaa actggcactg ggtgatccga aaaatgcaga tatgggtccg      960

ctgattaata aaggtgcact gcaggcaacc agcgaaattg ttgaagaagc gaaagaatct     1020

ggcgcaaaaa ttctgtttgg tggtagccag ccgagcctga gcggtccgta tcgtaatggc     1080

tatttttttc tgccgaccat tattggtaat gcggatcaga aaagcaaaat ctttcaggaa     1140

gaaatttttg caccggttat tggtgcacgt aaaattagca gcgtggaaga aatgtatgat     1200

ctggccaatg attgcaaata tggtctggcc agctacctgt ttaccaaaga tccgaatatc     1260

atttttgaag ccagcgaacg tattcgtttt ggtgaactgt atgtgaatat gccgggtccg     1320

gaagcaagcc agggttatca caccggtttt cgtatgacag gtcaggcagg cgaaggttct     1380

aaatatggca ttagcgaata tctgaaactg aaaaatattt atgtggatta tagcggcaaa     1440

ccgctgcata ttaataccgt tcgtgatgac ctgtttcaga gcgggagacc tgtgctgggc     1500

agcagccacc accaccacca ccactaa                                         1527


<210>  59
<211>  508
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TaALDH-S405C-Chis

<400>  59

Met Asp Thr Lys Leu Tyr Ile Asp Gly Gln Trp Val Asn Ser Ser Ser 
1               5                   10                  15      


Gly Lys Thr Val Asp Lys Tyr Ser Pro Val Thr Gly Gln Val Ile Gly 
            20                  25                  30          


Arg Phe Glu Ala Ala Thr Arg Asp Asp Val Asp Arg Ala Ile Asp Ala 
        35                  40                  45              


Ala Glu Asp Ala Phe Trp Ala Trp Asn Asp Leu Gly Ser Val Glu Arg 
    50                  55                  60                  


Ser Lys Ile Ile Tyr Arg Ala Lys Glu Leu Ile Glu Lys Asn Arg Ala 
65                  70                  75                  80  


Glu Leu Glu Asn Ile Ile Met Glu Glu Asn Gly Lys Pro Val Lys Glu 
                85                  90                  95      


Ala Lys Glu Glu Val Asp Gly Val Ile Asp Gln Ile Gln Tyr Tyr Ala 
            100                 105                 110         


Glu Trp Ala Arg Lys Leu Asn Gly Glu Val Val Glu Gly Thr Ser Ser 
        115                 120                 125             


His Arg Lys Ile Phe Gln Tyr Lys Val Pro Tyr Gly Ile Val Val Ala 
    130                 135                 140                 


Leu Thr Pro Trp Asn Phe Pro Ala Gly Met Val Ala Arg Lys Leu Ala 
145                 150                 155                 160 


Pro Ala Leu Leu Thr Gly Asn Thr Val Val Leu Lys Pro Ser Ser Asp 
                165                 170                 175     


Thr Pro Gly Ser Ala Glu Trp Ile Val Arg Lys Phe Val Glu Ala Gly 
            180                 185                 190         


Val Pro Lys Gly Val Leu Asn Phe Ile Thr Gly Arg Gly Ser Glu Ile 
        195                 200                 205             


Gly Asp Tyr Ile Val Glu His Lys Lys Val Asn Leu Ile Thr Met Thr 
    210                 215                 220                 


Gly Ser Thr Ala Thr Gly Gln Arg Ile Met Gln Lys Ala Ser Ala Asn 
225                 230                 235                 240 


Met Ala Lys Leu Ile Leu Glu Leu Gly Gly Lys Ala Pro Phe Met Val 
                245                 250                 255     


Trp Lys Asp Ala Asp Met Asp Asn Ala Leu Lys Thr Leu Leu Trp Ala 
            260                 265                 270         


Lys Tyr Trp Asn Ala Gly Gln Ser Cys Ile Ala Ala Glu Arg Leu Tyr 
        275                 280                 285             


Val His Glu Asp Ile Tyr Asp Thr Phe Met Ser Arg Phe Val Glu Leu 
    290                 295                 300                 


Ser Arg Lys Leu Ala Leu Gly Asp Pro Lys Asn Ala Asp Met Gly Pro 
305                 310                 315                 320 


Leu Ile Asn Lys Gly Ala Leu Gln Ala Thr Ser Glu Ile Val Glu Glu 
                325                 330                 335     


Ala Lys Glu Ser Gly Ala Lys Ile Leu Phe Gly Gly Ser Gln Pro Ser 
            340                 345                 350         


Leu Ser Gly Pro Tyr Arg Asn Gly Tyr Phe Phe Leu Pro Thr Ile Ile 
        355                 360                 365             


Gly Asn Ala Asp Gln Lys Ser Lys Ile Phe Gln Glu Glu Ile Phe Ala 
    370                 375                 380                 


Pro Val Ile Gly Ala Arg Lys Ile Ser Ser Val Glu Glu Met Tyr Asp 
385                 390                 395                 400 


Leu Ala Asn Asp Cys Lys Tyr Gly Leu Ala Ser Tyr Leu Phe Thr Lys 
                405                 410                 415     


Asp Pro Asn Ile Ile Phe Glu Ala Ser Glu Arg Ile Arg Phe Gly Glu 
            420                 425                 430         


Leu Tyr Val Asn Met Pro Gly Pro Glu Ala Ser Gln Gly Tyr His Thr 
        435                 440                 445             


Gly Phe Arg Met Thr Gly Gln Ala Gly Glu Gly Ser Lys Tyr Gly Ile 
    450                 455                 460                 


Ser Glu Tyr Leu Lys Leu Lys Asn Ile Tyr Val Asp Tyr Ser Gly Lys 
465                 470                 475                 480 


Pro Leu His Ile Asn Thr Val Arg Asp Asp Leu Phe Gln Ser Gly Arg 
                485                 490                 495     


Pro Val Leu Gly Ser Ser His His His His His His 
            500                 505             


<210>  60
<211>  1527
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TaALDH-F34L-S405C-Chis

<400>  60
atggatacca aactgtatat tgatggccag tgggttaata gcagcagcgg taaaaccgtt       60

gataaatatt ctccggttac cggtcaggtt attggtcgtt tagaagcagc aacccgtgat      120

gatgttgatc gtgcaattga tgcagcagaa gatgcatttt gggcctggaa tgatctgggt      180

agcgttgaac gcagcaaaat tatttatcgt gccaaagaac tgattgaaaa aaatcgtgcc      240

gaactggaaa atattattat ggaagaaaat ggcaaaccgg tgaaagaagc caaagaagaa      300

gtcgacggcg tcattgatca gattcagtat tatgcagaat gggcacgtaa actgaatggt      360

gaagttgttg aaggcaccag cagccatcgt aaaatttttc agtataaagt gccgtatggt      420

attgttgttg cactgacccc gtggaatttt ccggcaggca tggttgcccg taaactggca      480

ccggcactgc tgaccggtaa taccgttgtt ctgaaaccga gcagcgatac accgggtagc      540

gcagaatgga ttgtgcgtaa atttgttgaa gccggtgttc cgaaaggtgt gctgaatttt      600

attaccggtc gtggtagcga aattggcgat tacattgtgg aacataaaaa agtcaatctg      660

attaccatga ccggtagcac cgcaacaggt cagcgcatta tgcagaaagc aagcgcaaat      720

atggcaaaac tgattctgga actgggtggt aaagcaccgt ttatggtttg gaaagatgcc      780

gatatggata atgcactgaa aaccctgctg tgggcaaaat attggaatgc cggtcagagc      840

tgtattgcag cagaacgtct gtatgtgcat gaagatattt atgatacctt tatgagccgt      900

tttgttgaac tgagccgcaa actggcactg ggtgatccga aaaatgcaga tatgggtccg      960

ctgattaata aaggtgcact gcaggcaacc agcgaaattg ttgaagaagc gaaagaatct     1020

ggcgcaaaaa ttctgtttgg tggtagccag ccgagcctga gcggtccgta tcgtaatggc     1080

tatttttttc tgccgaccat tattggtaat gcggatcaga aaagcaaaat ctttcaggaa     1140

gaaatttttg caccggttat tggtgcacgt aaaattagca gcgtggaaga aatgtatgat     1200

ctggccaatg attgcaaata tggtctggcc agctacctgt ttaccaaaga tccgaatatc     1260

atttttgaag ccagcgaacg tattcgtttt ggtgaactgt atgtgaatat gccgggtccg     1320

gaagcaagcc agggttatca caccggtttt cgtatgacag gtcaggcagg cgaaggttct     1380

aaatatggca ttagcgaata tctgaaactg aaaaatattt atgtggatta tagcggcaaa     1440

ccgctgcata ttaataccgt tcgtgatgac ctgtttcaga gcgggagacc tgtgctgggc     1500

agcagccacc accaccacca ccactaa                                         1527


<210>  61
<211>  508
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TaALDH-F34L-S405C-Chis

<400>  61

Met Asp Thr Lys Leu Tyr Ile Asp Gly Gln Trp Val Asn Ser Ser Ser 
1               5                   10                  15      


Gly Lys Thr Val Asp Lys Tyr Ser Pro Val Thr Gly Gln Val Ile Gly 
            20                  25                  30          


Arg Leu Glu Ala Ala Thr Arg Asp Asp Val Asp Arg Ala Ile Asp Ala 
        35                  40                  45              


Ala Glu Asp Ala Phe Trp Ala Trp Asn Asp Leu Gly Ser Val Glu Arg 
    50                  55                  60                  


Ser Lys Ile Ile Tyr Arg Ala Lys Glu Leu Ile Glu Lys Asn Arg Ala 
65                  70                  75                  80  


Glu Leu Glu Asn Ile Ile Met Glu Glu Asn Gly Lys Pro Val Lys Glu 
                85                  90                  95      


Ala Lys Glu Glu Val Asp Gly Val Ile Asp Gln Ile Gln Tyr Tyr Ala 
            100                 105                 110         


Glu Trp Ala Arg Lys Leu Asn Gly Glu Val Val Glu Gly Thr Ser Ser 
        115                 120                 125             


His Arg Lys Ile Phe Gln Tyr Lys Val Pro Tyr Gly Ile Val Val Ala 
    130                 135                 140                 


Leu Thr Pro Trp Asn Phe Pro Ala Gly Met Val Ala Arg Lys Leu Ala 
145                 150                 155                 160 


Pro Ala Leu Leu Thr Gly Asn Thr Val Val Leu Lys Pro Ser Ser Asp 
                165                 170                 175     


Thr Pro Gly Ser Ala Glu Trp Ile Val Arg Lys Phe Val Glu Ala Gly 
            180                 185                 190         


Val Pro Lys Gly Val Leu Asn Phe Ile Thr Gly Arg Gly Ser Glu Ile 
        195                 200                 205             


Gly Asp Tyr Ile Val Glu His Lys Lys Val Asn Leu Ile Thr Met Thr 
    210                 215                 220                 


Gly Ser Thr Ala Thr Gly Gln Arg Ile Met Gln Lys Ala Ser Ala Asn 
225                 230                 235                 240 


Met Ala Lys Leu Ile Leu Glu Leu Gly Gly Lys Ala Pro Phe Met Val 
                245                 250                 255     


Trp Lys Asp Ala Asp Met Asp Asn Ala Leu Lys Thr Leu Leu Trp Ala 
            260                 265                 270         


Lys Tyr Trp Asn Ala Gly Gln Ser Cys Ile Ala Ala Glu Arg Leu Tyr 
        275                 280                 285             


Val His Glu Asp Ile Tyr Asp Thr Phe Met Ser Arg Phe Val Glu Leu 
    290                 295                 300                 


Ser Arg Lys Leu Ala Leu Gly Asp Pro Lys Asn Ala Asp Met Gly Pro 
305                 310                 315                 320 


Leu Ile Asn Lys Gly Ala Leu Gln Ala Thr Ser Glu Ile Val Glu Glu 
                325                 330                 335     


Ala Lys Glu Ser Gly Ala Lys Ile Leu Phe Gly Gly Ser Gln Pro Ser 
            340                 345                 350         


Leu Ser Gly Pro Tyr Arg Asn Gly Tyr Phe Phe Leu Pro Thr Ile Ile 
        355                 360                 365             


Gly Asn Ala Asp Gln Lys Ser Lys Ile Phe Gln Glu Glu Ile Phe Ala 
    370                 375                 380                 


Pro Val Ile Gly Ala Arg Lys Ile Ser Ser Val Glu Glu Met Tyr Asp 
385                 390                 395                 400 


Leu Ala Asn Asp Cys Lys Tyr Gly Leu Ala Ser Tyr Leu Phe Thr Lys 
                405                 410                 415     


Asp Pro Asn Ile Ile Phe Glu Ala Ser Glu Arg Ile Arg Phe Gly Glu 
            420                 425                 430         


Leu Tyr Val Asn Met Pro Gly Pro Glu Ala Ser Gln Gly Tyr His Thr 
        435                 440                 445             


Gly Phe Arg Met Thr Gly Gln Ala Gly Glu Gly Ser Lys Tyr Gly Ile 
    450                 455                 460                 


Ser Glu Tyr Leu Lys Leu Lys Asn Ile Tyr Val Asp Tyr Ser Gly Lys 
465                 470                 475                 480 


Pro Leu His Ile Asn Thr Val Arg Asp Asp Leu Phe Gln Ser Gly Arg 
                485                 490                 495     


Pro Val Leu Gly Ser Ser His His His His His His 
            500                 505             


<210>  62
<211>  1527
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TaALDH-F34M-S405N-Chis

<400>  62
atggatacca aactgtatat tgatggccag tgggttaata gcagcagcgg taaaaccgtt       60

gataaatatt ctccggttac cggtcaggtt attggtcgta tggaagcagc aacccgtgat      120

gatgttgatc gtgcaattga tgcagcagaa gatgcatttt gggcctggaa tgatctgggt      180

agcgttgaac gcagcaaaat tatttatcgt gccaaagaac tgattgaaaa aaatcgtgcc      240

gaactggaaa atattattat ggaagaaaat ggcaaaccgg tgaaagaagc caaagaagaa      300

gtcgacggcg tcattgatca gattcagtat tatgcagaat gggcacgtaa actgaatggt      360

gaagttgttg aaggcaccag cagccatcgt aaaatttttc agtataaagt gccgtatggt      420

attgttgttg cactgacccc gtggaatttt ccggcaggca tggttgcccg taaactggca      480

ccggcactgc tgaccggtaa taccgttgtt ctgaaaccga gcagcgatac accgggtagc      540

gcagaatgga ttgtgcgtaa atttgttgaa gccggtgttc cgaaaggtgt gctgaatttt      600

attaccggtc gtggtagcga aattggcgat tacattgtgg aacataaaaa agtcaatctg      660

attaccatga ccggtagcac cgcaacaggt cagcgcatta tgcagaaagc aagcgcaaat      720

atggcaaaac tgattctgga actgggtggt aaagcaccgt ttatggtttg gaaagatgcc      780

gatatggata atgcactgaa aaccctgctg tgggcaaaat attggaatgc cggtcagagc      840

tgtattgcag cagaacgtct gtatgtgcat gaagatattt atgatacctt tatgagccgt      900

tttgttgaac tgagccgcaa actggcactg ggtgatccga aaaatgcaga tatgggtccg      960

ctgattaata aaggtgcact gcaggcaacc agcgaaattg ttgaagaagc gaaagaatct     1020

ggcgcaaaaa ttctgtttgg tggtagccag ccgagcctga gcggtccgta tcgtaatggc     1080

tatttttttc tgccgaccat tattggtaat gcggatcaga aaagcaaaat ctttcaggaa     1140

gaaatttttg caccggttat tggtgcacgt aaaattagca gcgtggaaga aatgtatgat     1200

ctggccaatg ataacaaata tggtctggcc agctacctgt ttaccaaaga tccgaatatc     1260

atttttgaag ccagcgaacg tattcgtttt ggtgaactgt atgtgaatat gccgggtccg     1320

gaagcaagcc agggttatca caccggtttt cgtatgacag gtcaggcagg cgaaggttct     1380

aaatatggca ttagcgaata tctgaaactg aaaaatattt atgtggatta tagcggcaaa     1440

ccgctgcata ttaataccgt tcgtgatgac ctgtttcaga gcgggagacc tgtgctgggc     1500

agcagccacc accaccacca ccactaa                                         1527


<210>  63
<211>  508
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TaALDH-F34M-S405N-Chis

<400>  63

Met Asp Thr Lys Leu Tyr Ile Asp Gly Gln Trp Val Asn Ser Ser Ser 
1               5                   10                  15      


Gly Lys Thr Val Asp Lys Tyr Ser Pro Val Thr Gly Gln Val Ile Gly 
            20                  25                  30          


Arg Met Glu Ala Ala Thr Arg Asp Asp Val Asp Arg Ala Ile Asp Ala 
        35                  40                  45              


Ala Glu Asp Ala Phe Trp Ala Trp Asn Asp Leu Gly Ser Val Glu Arg 
    50                  55                  60                  


Ser Lys Ile Ile Tyr Arg Ala Lys Glu Leu Ile Glu Lys Asn Arg Ala 
65                  70                  75                  80  


Glu Leu Glu Asn Ile Ile Met Glu Glu Asn Gly Lys Pro Val Lys Glu 
                85                  90                  95      


Ala Lys Glu Glu Val Asp Gly Val Ile Asp Gln Ile Gln Tyr Tyr Ala 
            100                 105                 110         


Glu Trp Ala Arg Lys Leu Asn Gly Glu Val Val Glu Gly Thr Ser Ser 
        115                 120                 125             


His Arg Lys Ile Phe Gln Tyr Lys Val Pro Tyr Gly Ile Val Val Ala 
    130                 135                 140                 


Leu Thr Pro Trp Asn Phe Pro Ala Gly Met Val Ala Arg Lys Leu Ala 
145                 150                 155                 160 


Pro Ala Leu Leu Thr Gly Asn Thr Val Val Leu Lys Pro Ser Ser Asp 
                165                 170                 175     


Thr Pro Gly Ser Ala Glu Trp Ile Val Arg Lys Phe Val Glu Ala Gly 
            180                 185                 190         


Val Pro Lys Gly Val Leu Asn Phe Ile Thr Gly Arg Gly Ser Glu Ile 
        195                 200                 205             


Gly Asp Tyr Ile Val Glu His Lys Lys Val Asn Leu Ile Thr Met Thr 
    210                 215                 220                 


Gly Ser Thr Ala Thr Gly Gln Arg Ile Met Gln Lys Ala Ser Ala Asn 
225                 230                 235                 240 


Met Ala Lys Leu Ile Leu Glu Leu Gly Gly Lys Ala Pro Phe Met Val 
                245                 250                 255     


Trp Lys Asp Ala Asp Met Asp Asn Ala Leu Lys Thr Leu Leu Trp Ala 
            260                 265                 270         


Lys Tyr Trp Asn Ala Gly Gln Ser Cys Ile Ala Ala Glu Arg Leu Tyr 
        275                 280                 285             


Val His Glu Asp Ile Tyr Asp Thr Phe Met Ser Arg Phe Val Glu Leu 
    290                 295                 300                 


Ser Arg Lys Leu Ala Leu Gly Asp Pro Lys Asn Ala Asp Met Gly Pro 
305                 310                 315                 320 


Leu Ile Asn Lys Gly Ala Leu Gln Ala Thr Ser Glu Ile Val Glu Glu 
                325                 330                 335     


Ala Lys Glu Ser Gly Ala Lys Ile Leu Phe Gly Gly Ser Gln Pro Ser 
            340                 345                 350         


Leu Ser Gly Pro Tyr Arg Asn Gly Tyr Phe Phe Leu Pro Thr Ile Ile 
        355                 360                 365             


Gly Asn Ala Asp Gln Lys Ser Lys Ile Phe Gln Glu Glu Ile Phe Ala 
    370                 375                 380                 


Pro Val Ile Gly Ala Arg Lys Ile Ser Ser Val Glu Glu Met Tyr Asp 
385                 390                 395                 400 


Leu Ala Asn Asp Asn Lys Tyr Gly Leu Ala Ser Tyr Leu Phe Thr Lys 
                405                 410                 415     


Asp Pro Asn Ile Ile Phe Glu Ala Ser Glu Arg Ile Arg Phe Gly Glu 
            420                 425                 430         


Leu Tyr Val Asn Met Pro Gly Pro Glu Ala Ser Gln Gly Tyr His Thr 
        435                 440                 445             


Gly Phe Arg Met Thr Gly Gln Ala Gly Glu Gly Ser Lys Tyr Gly Ile 
    450                 455                 460                 


Ser Glu Tyr Leu Lys Leu Lys Asn Ile Tyr Val Asp Tyr Ser Gly Lys 
465                 470                 475                 480 


Pro Leu His Ile Asn Thr Val Arg Asp Asp Leu Phe Gln Ser Gly Arg 
                485                 490                 495     


Pro Val Leu Gly Ser Ser His His His His His His 
            500                 505             


<210>  64
<211>  1527
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TaALDH-W271S-Chis

<400>  64
atggatacca aactgtatat tgatggccag tgggttaata gcagcagcgg taaaaccgtt       60

gataaatatt ctccggttac cggtcaggtt attggtcgtt ttgaagcagc aacccgtgat      120

gatgttgatc gtgcaattga tgcagcagaa gatgcatttt gggcctggaa tgatctgggt      180

agcgttgaac gcagcaaaat tatttatcgt gccaaagaac tgattgaaaa aaatcgtgcc      240

gaactggaaa atattattat ggaagaaaat ggcaaaccgg tgaaagaagc caaagaagaa      300

gtcgacggcg tcattgatca gattcagtat tatgcagaat gggcacgtaa actgaatggt      360

gaagttgttg aaggcaccag cagccatcgt aaaatttttc agtataaagt gccgtatggt      420

attgttgttg cactgacccc gtggaatttt ccggcaggca tggttgcccg taaactggca      480

ccggcactgc tgaccggtaa taccgttgtt ctgaaaccga gcagcgatac accgggtagc      540

gcagaatgga ttgtgcgtaa atttgttgaa gccggtgttc cgaaaggtgt gctgaatttt      600

attaccggtc gtggtagcga aattggcgat tacattgtgg aacataaaaa agtcaatctg      660

attaccatga ccggtagcac cgcaacaggt cagcgcatta tgcagaaagc aagcgcaaat      720

atggcaaaac tgattctgga actgggtggt aaagcaccgt ttatggtttg gaaagatgcc      780

gatatggata atgcactgaa aaccctgctg tcggcaaaat attggaatgc cggtcagagc      840

tgtattgcag cagaacgtct gtatgtgcat gaagatattt atgatacctt tatgagccgt      900

tttgttgaac tgagccgcaa actggcactg ggtgatccga aaaatgcaga tatgggtccg      960

ctgattaata aaggtgcact gcaggcaacc agcgaaattg ttgaagaagc gaaagaatct     1020

ggcgcaaaaa ttctgtttgg tggtagccag ccgagcctga gcggtccgta tcgtaatggc     1080

tatttttttc tgccgaccat tattggtaat gcggatcaga aaagcaaaat ctttcaggaa     1140

gaaatttttg caccggttat tggtgcacgt aaaattagca gcgtggaaga aatgtatgat     1200

ctggccaatg atagcaaata tggtctggcc agctacctgt ttaccaaaga tccgaatatc     1260

atttttgaag ccagcgaacg tattcgtttt ggtgaactgt atgtgaatat gccgggtccg     1320

gaagcaagcc agggttatca caccggtttt cgtatgacag gtcaggcagg cgaaggttct     1380

aaatatggca ttagcgaata tctgaaactg aaaaatattt atgtggatta tagcggcaaa     1440

ccgctgcata ttaataccgt tcgtgatgac ctgtttcaga gcgggagacc tgtgctgggc     1500

agcagccacc accaccacca ccactaa                                         1527


<210>  65
<211>  508
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TaALDH-W271S-Chis

<400>  65

Met Asp Thr Lys Leu Tyr Ile Asp Gly Gln Trp Val Asn Ser Ser Ser 
1               5                   10                  15      


Gly Lys Thr Val Asp Lys Tyr Ser Pro Val Thr Gly Gln Val Ile Gly 
            20                  25                  30          


Arg Phe Glu Ala Ala Thr Arg Asp Asp Val Asp Arg Ala Ile Asp Ala 
        35                  40                  45              


Ala Glu Asp Ala Phe Trp Ala Trp Asn Asp Leu Gly Ser Val Glu Arg 
    50                  55                  60                  


Ser Lys Ile Ile Tyr Arg Ala Lys Glu Leu Ile Glu Lys Asn Arg Ala 
65                  70                  75                  80  


Glu Leu Glu Asn Ile Ile Met Glu Glu Asn Gly Lys Pro Val Lys Glu 
                85                  90                  95      


Ala Lys Glu Glu Val Asp Gly Val Ile Asp Gln Ile Gln Tyr Tyr Ala 
            100                 105                 110         


Glu Trp Ala Arg Lys Leu Asn Gly Glu Val Val Glu Gly Thr Ser Ser 
        115                 120                 125             


His Arg Lys Ile Phe Gln Tyr Lys Val Pro Tyr Gly Ile Val Val Ala 
    130                 135                 140                 


Leu Thr Pro Trp Asn Phe Pro Ala Gly Met Val Ala Arg Lys Leu Ala 
145                 150                 155                 160 


Pro Ala Leu Leu Thr Gly Asn Thr Val Val Leu Lys Pro Ser Ser Asp 
                165                 170                 175     


Thr Pro Gly Ser Ala Glu Trp Ile Val Arg Lys Phe Val Glu Ala Gly 
            180                 185                 190         


Val Pro Lys Gly Val Leu Asn Phe Ile Thr Gly Arg Gly Ser Glu Ile 
        195                 200                 205             


Gly Asp Tyr Ile Val Glu His Lys Lys Val Asn Leu Ile Thr Met Thr 
    210                 215                 220                 


Gly Ser Thr Ala Thr Gly Gln Arg Ile Met Gln Lys Ala Ser Ala Asn 
225                 230                 235                 240 


Met Ala Lys Leu Ile Leu Glu Leu Gly Gly Lys Ala Pro Phe Met Val 
                245                 250                 255     


Trp Lys Asp Ala Asp Met Asp Asn Ala Leu Lys Thr Leu Leu Ser Ala 
            260                 265                 270         


Lys Tyr Trp Asn Ala Gly Gln Ser Cys Ile Ala Ala Glu Arg Leu Tyr 
        275                 280                 285             


Val His Glu Asp Ile Tyr Asp Thr Phe Met Ser Arg Phe Val Glu Leu 
    290                 295                 300                 


Ser Arg Lys Leu Ala Leu Gly Asp Pro Lys Asn Ala Asp Met Gly Pro 
305                 310                 315                 320 


Leu Ile Asn Lys Gly Ala Leu Gln Ala Thr Ser Glu Ile Val Glu Glu 
                325                 330                 335     


Ala Lys Glu Ser Gly Ala Lys Ile Leu Phe Gly Gly Ser Gln Pro Ser 
            340                 345                 350         


Leu Ser Gly Pro Tyr Arg Asn Gly Tyr Phe Phe Leu Pro Thr Ile Ile 
        355                 360                 365             


Gly Asn Ala Asp Gln Lys Ser Lys Ile Phe Gln Glu Glu Ile Phe Ala 
    370                 375                 380                 


Pro Val Ile Gly Ala Arg Lys Ile Ser Ser Val Glu Glu Met Tyr Asp 
385                 390                 395                 400 


Leu Ala Asn Asp Ser Lys Tyr Gly Leu Ala Ser Tyr Leu Phe Thr Lys 
                405                 410                 415     


Asp Pro Asn Ile Ile Phe Glu Ala Ser Glu Arg Ile Arg Phe Gly Glu 
            420                 425                 430         


Leu Tyr Val Asn Met Pro Gly Pro Glu Ala Ser Gln Gly Tyr His Thr 
        435                 440                 445             


Gly Phe Arg Met Thr Gly Gln Ala Gly Glu Gly Ser Lys Tyr Gly Ile 
    450                 455                 460                 


Ser Glu Tyr Leu Lys Leu Lys Asn Ile Tyr Val Asp Tyr Ser Gly Lys 
465                 470                 475                 480 


Pro Leu His Ile Asn Thr Val Arg Asp Asp Leu Phe Gln Ser Gly Arg 
                485                 490                 495     


Pro Val Leu Gly Ser Ser His His His His His His 
            500                 505             


<210>  66
<211>  1527
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TaALDH-Y399R-Chis

<400>  66
atggatacca aactgtatat tgatggccag tgggttaata gcagcagcgg taaaaccgtt       60

gataaatatt ctccggttac cggtcaggtt attggtcgtt ttgaagcagc aacccgtgat      120

gatgttgatc gtgcaattga tgcagcagaa gatgcatttt gggcctggaa tgatctgggt      180

agcgttgaac gcagcaaaat tatttatcgt gccaaagaac tgattgaaaa aaatcgtgcc      240

gaactggaaa atattattat ggaagaaaat ggcaaaccgg tgaaagaagc caaagaagaa      300

gtcgacggcg tcattgatca gattcagtat tatgcagaat gggcacgtaa actgaatggt      360

gaagttgttg aaggcaccag cagccatcgt aaaatttttc agtataaagt gccgtatggt      420

attgttgttg cactgacccc gtggaatttt ccggcaggca tggttgcccg taaactggca      480

ccggcactgc tgaccggtaa taccgttgtt ctgaaaccga gcagcgatac accgggtagc      540

gcagaatgga ttgtgcgtaa atttgttgaa gccggtgttc cgaaaggtgt gctgaatttt      600

attaccggtc gtggtagcga aattggcgat tacattgtgg aacataaaaa agtcaatctg      660

attaccatga ccggtagcac cgcaacaggt cagcgcatta tgcagaaagc aagcgcaaat      720

atggcaaaac tgattctgga actgggtggt aaagcaccgt ttatggtttg gaaagatgcc      780

gatatggata atgcactgaa aaccctgctg tgggcaaaat attggaatgc cggtcagagc      840

tgtattgcag cagaacgtct gtatgtgcat gaagatattt atgatacctt tatgagccgt      900

tttgttgaac tgagccgcaa actggcactg ggtgatccga aaaatgcaga tatgggtccg      960

ctgattaata aaggtgcact gcaggcaacc agcgaaattg ttgaagaagc gaaagaatct     1020

ggcgcaaaaa ttctgtttgg tggtagccag ccgagcctga gcggtccgta tcgtaatggc     1080

tatttttttc tgccgaccat tattggtaat gcggatcaga aaagcaaaat ctttcaggaa     1140

gaaatttttg caccggttat tggtgcacgt aaaattagca gcgtggaaga aatgcgtgat     1200

ctggccaatg atagcaaata tggtctggcc agctacctgt ttaccaaaga tccgaatatc     1260

atttttgaag ccagcgaacg tattcgtttt ggtgaactgt atgtgaatat gccgggtccg     1320

gaagcaagcc agggttatca caccggtttt cgtatgacag gtcaggcagg cgaaggttct     1380

aaatatggca ttagcgaata tctgaaactg aaaaatattt atgtggatta tagcggcaaa     1440

ccgctgcata ttaataccgt tcgtgatgac ctgtttcaga gcgggagacc tgtgctgggc     1500

agcagccacc accaccacca ccactaa                                         1527


<210>  67
<211>  508
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TaALDH-Y399R-Chis

<400>  67

Met Asp Thr Lys Leu Tyr Ile Asp Gly Gln Trp Val Asn Ser Ser Ser 
1               5                   10                  15      


Gly Lys Thr Val Asp Lys Tyr Ser Pro Val Thr Gly Gln Val Ile Gly 
            20                  25                  30          


Arg Phe Glu Ala Ala Thr Arg Asp Asp Val Asp Arg Ala Ile Asp Ala 
        35                  40                  45              


Ala Glu Asp Ala Phe Trp Ala Trp Asn Asp Leu Gly Ser Val Glu Arg 
    50                  55                  60                  


Ser Lys Ile Ile Tyr Arg Ala Lys Glu Leu Ile Glu Lys Asn Arg Ala 
65                  70                  75                  80  


Glu Leu Glu Asn Ile Ile Met Glu Glu Asn Gly Lys Pro Val Lys Glu 
                85                  90                  95      


Ala Lys Glu Glu Val Asp Gly Val Ile Asp Gln Ile Gln Tyr Tyr Ala 
            100                 105                 110         


Glu Trp Ala Arg Lys Leu Asn Gly Glu Val Val Glu Gly Thr Ser Ser 
        115                 120                 125             


His Arg Lys Ile Phe Gln Tyr Lys Val Pro Tyr Gly Ile Val Val Ala 
    130                 135                 140                 


Leu Thr Pro Trp Asn Phe Pro Ala Gly Met Val Ala Arg Lys Leu Ala 
145                 150                 155                 160 


Pro Ala Leu Leu Thr Gly Asn Thr Val Val Leu Lys Pro Ser Ser Asp 
                165                 170                 175     


Thr Pro Gly Ser Ala Glu Trp Ile Val Arg Lys Phe Val Glu Ala Gly 
            180                 185                 190         


Val Pro Lys Gly Val Leu Asn Phe Ile Thr Gly Arg Gly Ser Glu Ile 
        195                 200                 205             


Gly Asp Tyr Ile Val Glu His Lys Lys Val Asn Leu Ile Thr Met Thr 
    210                 215                 220                 


Gly Ser Thr Ala Thr Gly Gln Arg Ile Met Gln Lys Ala Ser Ala Asn 
225                 230                 235                 240 


Met Ala Lys Leu Ile Leu Glu Leu Gly Gly Lys Ala Pro Phe Met Val 
                245                 250                 255     


Trp Lys Asp Ala Asp Met Asp Asn Ala Leu Lys Thr Leu Leu Trp Ala 
            260                 265                 270         


Lys Tyr Trp Asn Ala Gly Gln Ser Cys Ile Ala Ala Glu Arg Leu Tyr 
        275                 280                 285             


Val His Glu Asp Ile Tyr Asp Thr Phe Met Ser Arg Phe Val Glu Leu 
    290                 295                 300                 


Ser Arg Lys Leu Ala Leu Gly Asp Pro Lys Asn Ala Asp Met Gly Pro 
305                 310                 315                 320 


Leu Ile Asn Lys Gly Ala Leu Gln Ala Thr Ser Glu Ile Val Glu Glu 
                325                 330                 335     


Ala Lys Glu Ser Gly Ala Lys Ile Leu Phe Gly Gly Ser Gln Pro Ser 
            340                 345                 350         


Leu Ser Gly Pro Tyr Arg Asn Gly Tyr Phe Phe Leu Pro Thr Ile Ile 
        355                 360                 365             


Gly Asn Ala Asp Gln Lys Ser Lys Ile Phe Gln Glu Glu Ile Phe Ala 
    370                 375                 380                 


Pro Val Ile Gly Ala Arg Lys Ile Ser Ser Val Glu Glu Met Arg Asp 
385                 390                 395                 400 


Leu Ala Asn Asp Ser Lys Tyr Gly Leu Ala Ser Tyr Leu Phe Thr Lys 
                405                 410                 415     


Asp Pro Asn Ile Ile Phe Glu Ala Ser Glu Arg Ile Arg Phe Gly Glu 
            420                 425                 430         


Leu Tyr Val Asn Met Pro Gly Pro Glu Ala Ser Gln Gly Tyr His Thr 
        435                 440                 445             


Gly Phe Arg Met Thr Gly Gln Ala Gly Glu Gly Ser Lys Tyr Gly Ile 
    450                 455                 460                 


Ser Glu Tyr Leu Lys Leu Lys Asn Ile Tyr Val Asp Tyr Ser Gly Lys 
465                 470                 475                 480 


Pro Leu His Ile Asn Thr Val Arg Asp Asp Leu Phe Gln Ser Gly Arg 
                485                 490                 495     


Pro Val Leu Gly Ser Ser His His His His His His 
            500                 505             


<210>  68
<211>  1527
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TaALDH-F34M-W271S-Y399C-S405N-Chis

<400>  68
atggatacca aactgtatat tgatggccag tgggttaata gcagcagcgg taaaaccgtt       60

gataaatatt ctccggttac cggtcaggtt attggtcgta tggaagcagc aacccgtgat      120

gatgttgatc gtgcaattga tgcagcagaa gatgcatttt gggcctggaa tgatctgggt      180

agcgttgaac gcagcaaaat tatttatcgt gccaaagaac tgattgaaaa aaatcgtgcc      240

gaactggaaa atattattat ggaagaaaat ggcaaaccgg tgaaagaagc caaagaagaa      300

gtcgacggcg tcattgatca gattcagtat tatgcagaat gggcacgtaa actgaatggt      360

gaagttgttg aaggcaccag cagccatcgt aaaatttttc agtataaagt gccgtatggt      420

attgttgttg cactgacccc gtggaatttt ccggcaggca tggttgcccg taaactggca      480

ccggcactgc tgaccggtaa taccgttgtt ctgaaaccga gcagcgatac accgggtagc      540

gcagaatgga ttgtgcgtaa atttgttgaa gccggtgttc cgaaaggtgt gctgaatttt      600

attaccggtc gtggtagcga aattggcgat tacattgtgg aacataaaaa agtcaatctg      660

attaccatga ccggtagcac cgcaacaggt cagcgcatta tgcagaaagc aagcgcaaat      720

atggcaaaac tgattctgga actgggtggt aaagcaccgt ttatggtttg gaaagatgcc      780

gatatggata atgcactgaa aaccctgctg tcggcaaaat attggaatgc cggtcagagc      840

tgtattgcag cagaacgtct gtatgtgcat gaagatattt atgatacctt tatgagccgt      900

tttgttgaac tgagccgcaa actggcactg ggtgatccga aaaatgcaga tatgggtccg      960

ctgattaata aaggtgcact gcaggcaacc agcgaaattg ttgaagaagc gaaagaatct     1020

ggcgcaaaaa ttctgtttgg tggtagccag ccgagcctga gcggtccgta tcgtaatggc     1080

tatttttttc tgccgaccat tattggtaat gcggatcaga aaagcaaaat ctttcaggaa     1140

gaaatttttg caccggttat tggtgcacgt aaaattagca gcgtggaaga aatgtgtgat     1200

ctggccaatg ataacaaata tggtctggcc agctacctgt ttaccaaaga tccgaatatc     1260

atttttgaag ccagcgaacg tattcgtttt ggtgaactgt atgtgaatat gccgggtccg     1320

gaagcaagcc agggttatca caccggtttt cgtatgacag gtcaggcagg cgaaggttct     1380

aaatatggca ttagcgaata tctgaaactg aaaaatattt atgtggatta tagcggcaaa     1440

ccgctgcata ttaataccgt tcgtgatgac ctgtttcaga gcgggagacc tgtgctgggc     1500

agcagccacc accaccacca ccactaa                                         1527


<210>  69
<211>  508
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TaALDH-F34M-W271S-Y399C-S405N-Chis

<400>  69

Met Asp Thr Lys Leu Tyr Ile Asp Gly Gln Trp Val Asn Ser Ser Ser 
1               5                   10                  15      


Gly Lys Thr Val Asp Lys Tyr Ser Pro Val Thr Gly Gln Val Ile Gly 
            20                  25                  30          


Arg Met Glu Ala Ala Thr Arg Asp Asp Val Asp Arg Ala Ile Asp Ala 
        35                  40                  45              


Ala Glu Asp Ala Phe Trp Ala Trp Asn Asp Leu Gly Ser Val Glu Arg 
    50                  55                  60                  


Ser Lys Ile Ile Tyr Arg Ala Lys Glu Leu Ile Glu Lys Asn Arg Ala 
65                  70                  75                  80  


Glu Leu Glu Asn Ile Ile Met Glu Glu Asn Gly Lys Pro Val Lys Glu 
                85                  90                  95      


Ala Lys Glu Glu Val Asp Gly Val Ile Asp Gln Ile Gln Tyr Tyr Ala 
            100                 105                 110         


Glu Trp Ala Arg Lys Leu Asn Gly Glu Val Val Glu Gly Thr Ser Ser 
        115                 120                 125             


His Arg Lys Ile Phe Gln Tyr Lys Val Pro Tyr Gly Ile Val Val Ala 
    130                 135                 140                 


Leu Thr Pro Trp Asn Phe Pro Ala Gly Met Val Ala Arg Lys Leu Ala 
145                 150                 155                 160 


Pro Ala Leu Leu Thr Gly Asn Thr Val Val Leu Lys Pro Ser Ser Asp 
                165                 170                 175     


Thr Pro Gly Ser Ala Glu Trp Ile Val Arg Lys Phe Val Glu Ala Gly 
            180                 185                 190         


Val Pro Lys Gly Val Leu Asn Phe Ile Thr Gly Arg Gly Ser Glu Ile 
        195                 200                 205             


Gly Asp Tyr Ile Val Glu His Lys Lys Val Asn Leu Ile Thr Met Thr 
    210                 215                 220                 


Gly Ser Thr Ala Thr Gly Gln Arg Ile Met Gln Lys Ala Ser Ala Asn 
225                 230                 235                 240 


Met Ala Lys Leu Ile Leu Glu Leu Gly Gly Lys Ala Pro Phe Met Val 
                245                 250                 255     


Trp Lys Asp Ala Asp Met Asp Asn Ala Leu Lys Thr Leu Leu Ser Ala 
            260                 265                 270         


Lys Tyr Trp Asn Ala Gly Gln Ser Cys Ile Ala Ala Glu Arg Leu Tyr 
        275                 280                 285             


Val His Glu Asp Ile Tyr Asp Thr Phe Met Ser Arg Phe Val Glu Leu 
    290                 295                 300                 


Ser Arg Lys Leu Ala Leu Gly Asp Pro Lys Asn Ala Asp Met Gly Pro 
305                 310                 315                 320 


Leu Ile Asn Lys Gly Ala Leu Gln Ala Thr Ser Glu Ile Val Glu Glu 
                325                 330                 335     


Ala Lys Glu Ser Gly Ala Lys Ile Leu Phe Gly Gly Ser Gln Pro Ser 
            340                 345                 350         


Leu Ser Gly Pro Tyr Arg Asn Gly Tyr Phe Phe Leu Pro Thr Ile Ile 
        355                 360                 365             


Gly Asn Ala Asp Gln Lys Ser Lys Ile Phe Gln Glu Glu Ile Phe Ala 
    370                 375                 380                 


Pro Val Ile Gly Ala Arg Lys Ile Ser Ser Val Glu Glu Met Cys Asp 
385                 390                 395                 400 


Leu Ala Asn Asp Asn Lys Tyr Gly Leu Ala Ser Tyr Leu Phe Thr Lys 
                405                 410                 415     


Asp Pro Asn Ile Ile Phe Glu Ala Ser Glu Arg Ile Arg Phe Gly Glu 
            420                 425                 430         


Leu Tyr Val Asn Met Pro Gly Pro Glu Ala Ser Gln Gly Tyr His Thr 
        435                 440                 445             


Gly Phe Arg Met Thr Gly Gln Ala Gly Glu Gly Ser Lys Tyr Gly Ile 
    450                 455                 460                 


Ser Glu Tyr Leu Lys Leu Lys Asn Ile Tyr Val Asp Tyr Ser Gly Lys 
465                 470                 475                 480 


Pro Leu His Ile Asn Thr Val Arg Asp Asp Leu Phe Gln Ser Gly Arg 
                485                 490                 495     


Pro Val Leu Gly Ser Ser His His His His His His 
            500                 505             


