                         SEQUENCE LISTING

<110>  BASF SE
 
<120>  Enzymatic Amination of Alcohols

<130>  M/53272

<160>  70    

<170>  PatentIn version 3.3

<210>  1
<211>  1362
<212>  DNA
<213>  Vibrio fluvialis

<400>  1
atgaacaaac cgcaaagctg ggaagcccgg gccgagacct attcgctcta tggtttcacc     60

gacatgcctt cgctgcatca gcgcggcacg gtcgtcgtga cccatggcga gggaccctat    120

atcgtcgatg tgaatggccg gcgttatctg gacgccaact cgggcctgtg gaacatggtc    180

gcgggctttg accacaaggg gctgatcgac gccgccaagg cccaatacga gcgttttccc    240

ggttatcacg cctttttcgg ccgcatgtcc gatcagacgg taatgctgtc ggaaaagctg    300

gtcgaggtgt cgccctttga ttcgggccgg gtgttctata caaactcggg gtccgaggcg    360

aatgacacca tggtcaagat gctatggttc ctgcatgcag ccgagggcaa accgcaaaag    420

cgcaagatcc tgacccgctg gaacgcctat cacggcgtga ccgccgtttc ggccagcatg    480

accggcaagc cctataattc ggtctttggc ctgccgctgc cgggctttgt gcatctgacc    540

tgcccgcatt actggcgcta tggcgaagag ggcgaaaccg aagagcagtt cgtcgcccgc    600

ctcgcccgcg agctggagga aacgatccag cgcgagggcg ccgacaccat cgccggtttc    660

tttgccgaac cggtgatggg cgcgggcggc gtgattcccc cggccaaggg gtatttccag    720

gcgatcctgc caatcctgcg caaatatgac atcccggtca tctcggacga ggtgatctgc    780

ggtttcggac gcaccggtaa cacctggggc tgcgtgacct atgactttac acccgatgca    840

atcatctcgt ccaagaatct tacagcgggc tttttcccca tgggggcggt gatccttggc    900

ccggaacttt ccaaacggct ggaaaccgca atcgaggcga tcgaggaatt cccccatggc    960

tttaccgcct cgggccatcc ggtcggctgt gctattgcgc tgaaagcaat cgacgtggtg   1020

atgaatgaag ggctggctga gaacgtccgc cgccttgccc cccgtttcga ggaaaggctg   1080

aaacatatcg ccgagcgccc gaacatcggt gaatatcgcg gcatcggctt catgtgggcg   1140

ctggaggctg tcaaggacaa ggcaagcaag acgccgttcg acggcaacct gtcggtcagc   1200

gagcgtatcg ccaatacctg caccgatctg gggctgattt gccggccgct tggtcagtcc   1260

gtcgtccttt gtccgccctt tatcctgacc gaggcgcaga tggatgagat gttcgataaa   1320

ctcgaaaaag cccttgataa ggtctttgcc gaggttgcct ga                      1362


<210>  2
<211>  1362
<212>  DNA
<213>  Vibrio fluvialis


<220>
<221>  CDS
<222>  (1)..(1362)

<400>  2
atg aat aaa ccg caa agc tgg gaa gcg cgt gcg gaa acg tac agc ttg       48
Met Asn Lys Pro Gln Ser Trp Glu Ala Arg Ala Glu Thr Tyr Ser Leu         
1               5                   10                  15              

tac ggc ttt acc gat atg ccg agc ctg cac cag cgc ggt acc gtt gtc       96
Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val         
            20                  25                  30                  

gtc acg cat ggt gag ggc ccg tat att gtc gat gtg aat ggc cgt cgt      144
Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val Asn Gly Arg Arg         
        35                  40                  45                      

tac ttg gat gcg aat tct ggt ctg tgg aac atg gtt gct ggt ttt gat      192
Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp         
    50                  55                  60                          

cac aag ggc ctg att gac gcg gcg aag gcc cag tat gag cgt ttt ccg      240
His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro         
65                  70                  75                  80          

ggt tat cac gcg ttc ttc ggc cgt atg agc gac cag acc gtc atg ttg      288
Gly Tyr His Ala Phe Phe Gly Arg Met Ser Asp Gln Thr Val Met Leu         
                85                  90                  95              

agc gaa aaa ctg gtc gag gtt tcg ccg ttc gac agc ggt cgc gtt ttc      336
Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe         
            100                 105                 110                 

tat acc aac agc ggt agc gag gcg aac gat acc atg gtt aag atg ctg      384
Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu         
        115                 120                 125                     

tgg ttc ctg cat gcc gcg gaa ggt aaa cct cag aag cgc aag att ttg      432
Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu         
    130                 135                 140                         

acg cgc tgg aac gcg tat cac ggt gtc acg gcg gtg agc gcg agc atg      480
Thr Arg Trp Asn Ala Tyr His Gly Val Thr Ala Val Ser Ala Ser Met         
145                 150                 155                 160         

acg ggt aaa ccg tac aat tcc gtg ttt ggt ctg cct ctg ccg ggt ttt      528
Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe         
                165                 170                 175             

gtt cac ctg acg tgc ccg cac tac tgg cgc tac ggc gaa gag ggt gag      576
Val His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu         
            180                 185                 190                 

act gaa gag caa ttt gtc gcc cgt ctg gcg cgt gaa ctg gaa gaa acc      624
Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr         
        195                 200                 205                     

atc cag cgc gaa ggc gcg gac act atc gcc ggt ttc ttt gcc gag ccg      672
Ile Gln Arg Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro         
    210                 215                 220                         

gtt atg ggt gct ggt ggc gtg atc ccg cca gcg aaa ggt tat ttc caa      720
Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln         
225                 230                 235                 240         

gca atc ctg ccg att ctg cgt aag tac gac att ccg gtg att agc gat      768
Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp         
                245                 250                 255             

gag gtg atc tgt ggc ttc ggt cgt acc ggt aat acc tgg ggt tgt gtg      816
Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val         
            260                 265                 270                 

acc tac gat ttc acc cca gac gca atc atc tct agc aaa aac ctg act      864
Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr         
        275                 280                 285                     

gca ggt ttc ttt cca atg ggc gct gtc atc ctg ggt ccg gag ctg agc      912
Ala Gly Phe Phe Pro Met Gly Ala Val Ile Leu Gly Pro Glu Leu Ser         
    290                 295                 300                         

aag cgt ctg gaa acg gcc atc gag gcc att gaa gag ttc ccg cac ggt      960
Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly         
305                 310                 315                 320         

ttc acc gcg agc ggc cat ccg gtg ggc tgc gcg att gcg ctg aaa gcg     1008
Phe Thr Ala Ser Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala         
                325                 330                 335             

att gac gtg gtt atg aac gaa ggt ctg gca gaa aac gtc cgt cgt ttg     1056
Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu         
            340                 345                 350                 

gca ccg cgc ttt gaa gag cgc ctg aaa cat att gca gag cgt ccg aac     1104
Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn         
        355                 360                 365                     

atc ggt gag tac cgt ggc att ggt ttt atg tgg gcg ctg gaa gca gtg     1152
Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val         
    370                 375                 380                         

aag gac aaa gct tcc aag acc ccg ttc gac ggc aat ctg agc gtg tct     1200
Lys Asp Lys Ala Ser Lys Thr Pro Phe Asp Gly Asn Leu Ser Val Ser         
385                 390                 395                 400         

gag cgc att gcg aat acg tgc acc gat ctg ggc ctg atc tgc cgt ccg     1248
Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro         
                405                 410                 415             

ttg ggc caa tcc gtt gtt ctg tgt ccg ccg ttt atc ctg acc gag gca     1296
Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala         
            420                 425                 430                 

cag atg gac gag atg ttt gat aaa ctg gag aaa gca ctg gac aag gtg     1344
Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val         
        435                 440                 445                     

ttc gct gag gtt gcc taa                                             1362
Phe Ala Glu Val Ala                                                     
    450                                                                 


<210>  3
<211>  453
<212>  PRT
<213>  Vibrio fluvialis

<400>  3

Met Asn Lys Pro Gln Ser Trp Glu Ala Arg Ala Glu Thr Tyr Ser Leu 
1               5                   10                  15      


Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg Gly Thr Val Val 
            20                  25                  30          


Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val Asn Gly Arg Arg 
        35                  40                  45              


Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val Ala Gly Phe Asp 
    50                  55                  60                  


His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr Glu Arg Phe Pro 
65                  70                  75                  80  


Gly Tyr His Ala Phe Phe Gly Arg Met Ser Asp Gln Thr Val Met Leu 
                85                  90                  95      


Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser Gly Arg Val Phe 
            100                 105                 110         


Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met Val Lys Met Leu 
        115                 120                 125             


Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys Arg Lys Ile Leu 
    130                 135                 140                 


Thr Arg Trp Asn Ala Tyr His Gly Val Thr Ala Val Ser Ala Ser Met 
145                 150                 155                 160 


Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro Leu Pro Gly Phe 
                165                 170                 175     


Val His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly Glu Glu Gly Glu 
            180                 185                 190         


Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu Leu Glu Glu Thr 
        195                 200                 205             


Ile Gln Arg Glu Gly Ala Asp Thr Ile Ala Gly Phe Phe Ala Glu Pro 
    210                 215                 220                 


Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys Gly Tyr Phe Gln 
225                 230                 235                 240 


Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro Val Ile Ser Asp 
                245                 250                 255     


Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr Trp Gly Cys Val 
            260                 265                 270         


Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser Lys Asn Leu Thr 
        275                 280                 285             


Ala Gly Phe Phe Pro Met Gly Ala Val Ile Leu Gly Pro Glu Leu Ser 
    290                 295                 300                 


Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu Phe Pro His Gly 
305                 310                 315                 320 


Phe Thr Ala Ser Gly His Pro Val Gly Cys Ala Ile Ala Leu Lys Ala 
                325                 330                 335     


Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn Val Arg Arg Leu 
            340                 345                 350         


Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala Glu Arg Pro Asn 
        355                 360                 365             


Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala Leu Glu Ala Val 
    370                 375                 380                 


Lys Asp Lys Ala Ser Lys Thr Pro Phe Asp Gly Asn Leu Ser Val Ser 
385                 390                 395                 400 


Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu Ile Cys Arg Pro 
                405                 410                 415     


Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile Leu Thr Glu Ala 
            420                 425                 430         


Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala Leu Asp Lys Val 
        435                 440                 445             


Phe Ala Glu Val Ala 
    450             


<210>  4
<211>  473
<212>  PRT
<213>  Artificial

<220>
<223>  Vf-omegaTA with Hs Tag

<400>  4

Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His Met Asn Lys Pro Gln Ser Trp Glu Ala Arg Ala Glu 
            20                  25                  30          


Thr Tyr Ser Leu Tyr Gly Phe Thr Asp Met Pro Ser Leu His Gln Arg 
        35                  40                  45              


Gly Thr Val Val Val Thr His Gly Glu Gly Pro Tyr Ile Val Asp Val 
    50                  55                  60                  


Asn Gly Arg Arg Tyr Leu Asp Ala Asn Ser Gly Leu Trp Asn Met Val 
65                  70                  75                  80  


Ala Gly Phe Asp His Lys Gly Leu Ile Asp Ala Ala Lys Ala Gln Tyr 
                85                  90                  95      


Glu Arg Phe Pro Gly Tyr His Ala Phe Phe Gly Arg Met Ser Asp Gln 
            100                 105                 110         


Thr Val Met Leu Ser Glu Lys Leu Val Glu Val Ser Pro Phe Asp Ser 
        115                 120                 125             


Gly Arg Val Phe Tyr Thr Asn Ser Gly Ser Glu Ala Asn Asp Thr Met 
    130                 135                 140                 


Val Lys Met Leu Trp Phe Leu His Ala Ala Glu Gly Lys Pro Gln Lys 
145                 150                 155                 160 


Arg Lys Ile Leu Thr Arg Trp Asn Ala Tyr His Gly Val Thr Ala Val 
                165                 170                 175     


Ser Ala Ser Met Thr Gly Lys Pro Tyr Asn Ser Val Phe Gly Leu Pro 
            180                 185                 190         


Leu Pro Gly Phe Val His Leu Thr Cys Pro His Tyr Trp Arg Tyr Gly 
        195                 200                 205             


Glu Glu Gly Glu Thr Glu Glu Gln Phe Val Ala Arg Leu Ala Arg Glu 
    210                 215                 220                 


Leu Glu Glu Thr Ile Gln Arg Glu Gly Ala Asp Thr Ile Ala Gly Phe 
225                 230                 235                 240 


Phe Ala Glu Pro Val Met Gly Ala Gly Gly Val Ile Pro Pro Ala Lys 
                245                 250                 255     


Gly Tyr Phe Gln Ala Ile Leu Pro Ile Leu Arg Lys Tyr Asp Ile Pro 
            260                 265                 270         


Val Ile Ser Asp Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Asn Thr 
        275                 280                 285             


Trp Gly Cys Val Thr Tyr Asp Phe Thr Pro Asp Ala Ile Ile Ser Ser 
    290                 295                 300                 


Lys Asn Leu Thr Ala Gly Phe Phe Pro Met Gly Ala Val Ile Leu Gly 
305                 310                 315                 320 


Pro Glu Leu Ser Lys Arg Leu Glu Thr Ala Ile Glu Ala Ile Glu Glu 
                325                 330                 335     


Phe Pro His Gly Phe Thr Ala Ser Gly His Pro Val Gly Cys Ala Ile 
            340                 345                 350         


Ala Leu Lys Ala Ile Asp Val Val Met Asn Glu Gly Leu Ala Glu Asn 
        355                 360                 365             


Val Arg Arg Leu Ala Pro Arg Phe Glu Glu Arg Leu Lys His Ile Ala 
    370                 375                 380                 


Glu Arg Pro Asn Ile Gly Glu Tyr Arg Gly Ile Gly Phe Met Trp Ala 
385                 390                 395                 400 


Leu Glu Ala Val Lys Asp Lys Ala Ser Lys Thr Pro Phe Asp Gly Asn 
                405                 410                 415     


Leu Ser Val Ser Glu Arg Ile Ala Asn Thr Cys Thr Asp Leu Gly Leu 
            420                 425                 430         


Ile Cys Arg Pro Leu Gly Gln Ser Val Val Leu Cys Pro Pro Phe Ile 
        435                 440                 445             


Leu Thr Glu Ala Gln Met Asp Glu Met Phe Asp Lys Leu Glu Lys Ala 
    450                 455                 460                 


Leu Asp Lys Val Phe Ala Glu Val Ala 
465                 470             


<210>  5
<211>  1380
<212>  DNA
<213>  Chromobacterium violaceum


<220>
<221>  CDS
<222>  (1)..(1380)

<400>  5
atg cag aag caa cgt acg acc agc caa tgg cgc gaa ctg gat gcc gcc       48
Met Gln Lys Gln Arg Thr Thr Ser Gln Trp Arg Glu Leu Asp Ala Ala         
1               5                   10                  15              

cat cac ctg cat ccg ttc acc gat acc gca tcg ctg aac cag gcg ggc       96
His His Leu His Pro Phe Thr Asp Thr Ala Ser Leu Asn Gln Ala Gly         
            20                  25                  30                  

gcg cgc gtg atg acg cgc gga gag ggc gtc tac ctg tgg gat tcg gaa      144
Ala Arg Val Met Thr Arg Gly Glu Gly Val Tyr Leu Trp Asp Ser Glu         
        35                  40                  45                      

ggc aac aag atc atc gac ggc atg gcc gga ctg tgg tgc gtg aac gtc      192
Gly Asn Lys Ile Ile Asp Gly Met Ala Gly Leu Trp Cys Val Asn Val         
    50                  55                  60                          

ggc tac ggc cgc aag gac ttt gcc gaa gcg gcg cgc cgg cag atg gaa      240
Gly Tyr Gly Arg Lys Asp Phe Ala Glu Ala Ala Arg Arg Gln Met Glu         
65                  70                  75                  80          

gag ctg ccg ttc tac aac acc ttc ttc aag acc acc cat ccg gcg gtg      288
Glu Leu Pro Phe Tyr Asn Thr Phe Phe Lys Thr Thr His Pro Ala Val         
                85                  90                  95              

gtc gag ctg tcc agc ctg ctg gct gaa gtg acg ccg gcc ggt ttc gac      336
Val Glu Leu Ser Ser Leu Leu Ala Glu Val Thr Pro Ala Gly Phe Asp         
            100                 105                 110                 

cgc gtg ttc tat acc aat tcc ggt tcc gaa tcg gtg gac acc atg atc      384
Arg Val Phe Tyr Thr Asn Ser Gly Ser Glu Ser Val Asp Thr Met Ile         
        115                 120                 125                     

cgc atg gtg cgc cgc tac tgg gac gtg cag ggc aag ccg gag aag aag      432
Arg Met Val Arg Arg Tyr Trp Asp Val Gln Gly Lys Pro Glu Lys Lys         
    130                 135                 140                         

acg ctg atc ggc cgc tgg aac ggc tat cac ggc tcc acc atc ggc ggc      480
Thr Leu Ile Gly Arg Trp Asn Gly Tyr His Gly Ser Thr Ile Gly Gly         
145                 150                 155                 160         

gcc agc ctg ggc ggc atg aag tac atg cac gag cag ggc gac ttg ccg      528
Ala Ser Leu Gly Gly Met Lys Tyr Met His Glu Gln Gly Asp Leu Pro         
                165                 170                 175             

att ccg ggc atg gcc cac atc gag cag cct tgg tgg tac aag cac ggc      576
Ile Pro Gly Met Ala His Ile Glu Gln Pro Trp Trp Tyr Lys His Gly         
            180                 185                 190                 

aag gac atg acg ccg gac gag ttc ggc gtg gtg gcc gcg cgc tgg ctg      624
Lys Asp Met Thr Pro Asp Glu Phe Gly Val Val Ala Ala Arg Trp Leu         
        195                 200                 205                     

gaa gag aag att ctg gaa atc ggc gcc gac aag gtg gcc gcc ttc gtc      672
Glu Glu Lys Ile Leu Glu Ile Gly Ala Asp Lys Val Ala Ala Phe Val         
    210                 215                 220                         

ggc gaa ccc atc cag ggc gcc ggc ggc gtg atc gtc ccg ccg gcc acc      720
Gly Glu Pro Ile Gln Gly Ala Gly Gly Val Ile Val Pro Pro Ala Thr         
225                 230                 235                 240         

tac tgg ccg gaa atc gag cgc att tgc cgc aag tac gac gtg ctg ctg      768
Tyr Trp Pro Glu Ile Glu Arg Ile Cys Arg Lys Tyr Asp Val Leu Leu         
                245                 250                 255             

gtg gcc gac gaa gtg atc tgc ggc ttc ggg cgt acc ggc gaa tgg ttc      816
Val Ala Asp Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Glu Trp Phe         
            260                 265                 270                 

ggc cat cag cat ttc ggc ttc cag ccc gac ctg ttc acc gcc gcc aag      864
Gly His Gln His Phe Gly Phe Gln Pro Asp Leu Phe Thr Ala Ala Lys         
        275                 280                 285                     

ggc ctg tcc tcc ggc tat ctg ccg ata ggc gcg gtc ttt gtc ggc aag      912
Gly Leu Ser Ser Gly Tyr Leu Pro Ile Gly Ala Val Phe Val Gly Lys         
    290                 295                 300                         

cgc gtg gcc gaa ggc ctg atc gcc ggc ggc gac ttc aac cac ggc ttc      960
Arg Val Ala Glu Gly Leu Ile Ala Gly Gly Asp Phe Asn His Gly Phe         
305                 310                 315                 320         

acc tac tcc ggc cac ccg gtc tgc gcc gcc gtc gcc cac gcc aac gtg     1008
Thr Tyr Ser Gly His Pro Val Cys Ala Ala Val Ala His Ala Asn Val         
                325                 330                 335             

gcg gcg ctg cgc gac gag ggc atc gtc cag cgc gtc aag gac gac atc     1056
Ala Ala Leu Arg Asp Glu Gly Ile Val Gln Arg Val Lys Asp Asp Ile         
            340                 345                 350                 

ggc ccg tac atg caa aag cgc tgg cgt gaa acc ttc agc cgt ttc gag     1104
Gly Pro Tyr Met Gln Lys Arg Trp Arg Glu Thr Phe Ser Arg Phe Glu         
        355                 360                 365                     

cat gtg gac gac gtg cgc ggc gtc ggc atg gtg cag gcg ttc acc ctg     1152
His Val Asp Asp Val Arg Gly Val Gly Met Val Gln Ala Phe Thr Leu         
    370                 375                 380                         

gtg aag aac aag gcg aag cgc gag ctg ttc ccc gat ttc ggc gag atc     1200
Val Lys Asn Lys Ala Lys Arg Glu Leu Phe Pro Asp Phe Gly Glu Ile         
385                 390                 395                 400         

ggc acg ctg tgc cgc gac atc ttc ttc cgc aac aac ctg atc atg cgg     1248
Gly Thr Leu Cys Arg Asp Ile Phe Phe Arg Asn Asn Leu Ile Met Arg         
                405                 410                 415             

gca tgc ggc gac cac atc gtg tcg gcg ccg ccg ctg gtg atg acg cgg     1296
Ala Cys Gly Asp His Ile Val Ser Ala Pro Pro Leu Val Met Thr Arg         
            420                 425                 430                 

gcg gaa gtg gac gag atg ctg gcg gtg gcg gaa cgc tgt ctg gag gaa     1344
Ala Glu Val Asp Glu Met Leu Ala Val Ala Glu Arg Cys Leu Glu Glu         
        435                 440                 445                     

ttc gag cag acg ctg aag gcg cgc ggg ctg gct tag                     1380
Phe Glu Gln Thr Leu Lys Ala Arg Gly Leu Ala                             
    450                 455                                             


<210>  6
<211>  459
<212>  PRT
<213>  Chromobacterium violaceum

<400>  6

Met Gln Lys Gln Arg Thr Thr Ser Gln Trp Arg Glu Leu Asp Ala Ala 
1               5                   10                  15      


His His Leu His Pro Phe Thr Asp Thr Ala Ser Leu Asn Gln Ala Gly 
            20                  25                  30          


Ala Arg Val Met Thr Arg Gly Glu Gly Val Tyr Leu Trp Asp Ser Glu 
        35                  40                  45              


Gly Asn Lys Ile Ile Asp Gly Met Ala Gly Leu Trp Cys Val Asn Val 
    50                  55                  60                  


Gly Tyr Gly Arg Lys Asp Phe Ala Glu Ala Ala Arg Arg Gln Met Glu 
65                  70                  75                  80  


Glu Leu Pro Phe Tyr Asn Thr Phe Phe Lys Thr Thr His Pro Ala Val 
                85                  90                  95      


Val Glu Leu Ser Ser Leu Leu Ala Glu Val Thr Pro Ala Gly Phe Asp 
            100                 105                 110         


Arg Val Phe Tyr Thr Asn Ser Gly Ser Glu Ser Val Asp Thr Met Ile 
        115                 120                 125             


Arg Met Val Arg Arg Tyr Trp Asp Val Gln Gly Lys Pro Glu Lys Lys 
    130                 135                 140                 


Thr Leu Ile Gly Arg Trp Asn Gly Tyr His Gly Ser Thr Ile Gly Gly 
145                 150                 155                 160 


Ala Ser Leu Gly Gly Met Lys Tyr Met His Glu Gln Gly Asp Leu Pro 
                165                 170                 175     


Ile Pro Gly Met Ala His Ile Glu Gln Pro Trp Trp Tyr Lys His Gly 
            180                 185                 190         


Lys Asp Met Thr Pro Asp Glu Phe Gly Val Val Ala Ala Arg Trp Leu 
        195                 200                 205             


Glu Glu Lys Ile Leu Glu Ile Gly Ala Asp Lys Val Ala Ala Phe Val 
    210                 215                 220                 


Gly Glu Pro Ile Gln Gly Ala Gly Gly Val Ile Val Pro Pro Ala Thr 
225                 230                 235                 240 


Tyr Trp Pro Glu Ile Glu Arg Ile Cys Arg Lys Tyr Asp Val Leu Leu 
                245                 250                 255     


Val Ala Asp Glu Val Ile Cys Gly Phe Gly Arg Thr Gly Glu Trp Phe 
            260                 265                 270         


Gly His Gln His Phe Gly Phe Gln Pro Asp Leu Phe Thr Ala Ala Lys 
        275                 280                 285             


Gly Leu Ser Ser Gly Tyr Leu Pro Ile Gly Ala Val Phe Val Gly Lys 
    290                 295                 300                 


Arg Val Ala Glu Gly Leu Ile Ala Gly Gly Asp Phe Asn His Gly Phe 
305                 310                 315                 320 


Thr Tyr Ser Gly His Pro Val Cys Ala Ala Val Ala His Ala Asn Val 
                325                 330                 335     


Ala Ala Leu Arg Asp Glu Gly Ile Val Gln Arg Val Lys Asp Asp Ile 
            340                 345                 350         


Gly Pro Tyr Met Gln Lys Arg Trp Arg Glu Thr Phe Ser Arg Phe Glu 
        355                 360                 365             


His Val Asp Asp Val Arg Gly Val Gly Met Val Gln Ala Phe Thr Leu 
    370                 375                 380                 


Val Lys Asn Lys Ala Lys Arg Glu Leu Phe Pro Asp Phe Gly Glu Ile 
385                 390                 395                 400 


Gly Thr Leu Cys Arg Asp Ile Phe Phe Arg Asn Asn Leu Ile Met Arg 
                405                 410                 415     


Ala Cys Gly Asp His Ile Val Ser Ala Pro Pro Leu Val Met Thr Arg 
            420                 425                 430         


Ala Glu Val Asp Glu Met Leu Ala Val Ala Glu Arg Cys Leu Glu Glu 
        435                 440                 445             


Phe Glu Gln Thr Leu Lys Ala Arg Gly Leu Ala 
    450                 455                 


<210>  7
<211>  479
<212>  PRT
<213>  Artificial

<220>
<223>  Cv-omega TA + His Tag

<400>  7

Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His Met Gln Lys Gln Arg Thr Thr Ser Gln Trp Arg Glu 
            20                  25                  30          


Leu Asp Ala Ala His His Leu His Pro Phe Thr Asp Thr Ala Ser Leu 
        35                  40                  45              


Asn Gln Ala Gly Ala Arg Val Met Thr Arg Gly Glu Gly Val Tyr Leu 
    50                  55                  60                  


Trp Asp Ser Glu Gly Asn Lys Ile Ile Asp Gly Met Ala Gly Leu Trp 
65                  70                  75                  80  


Cys Val Asn Val Gly Tyr Gly Arg Lys Asp Phe Ala Glu Ala Ala Arg 
                85                  90                  95      


Arg Gln Met Glu Glu Leu Pro Phe Tyr Asn Thr Phe Phe Lys Thr Thr 
            100                 105                 110         


His Pro Ala Val Val Glu Leu Ser Ser Leu Leu Ala Glu Val Thr Pro 
        115                 120                 125             


Ala Gly Phe Asp Arg Val Phe Tyr Thr Asn Ser Gly Ser Glu Ser Val 
    130                 135                 140                 


Asp Thr Met Ile Arg Met Val Arg Arg Tyr Trp Asp Val Gln Gly Lys 
145                 150                 155                 160 


Pro Glu Lys Lys Thr Leu Ile Gly Arg Trp Asn Gly Tyr His Gly Ser 
                165                 170                 175     


Thr Ile Gly Gly Ala Ser Leu Gly Gly Met Lys Tyr Met His Glu Gln 
            180                 185                 190         


Gly Asp Leu Pro Ile Pro Gly Met Ala His Ile Glu Gln Pro Trp Trp 
        195                 200                 205             


Tyr Lys His Gly Lys Asp Met Thr Pro Asp Glu Phe Gly Val Val Ala 
    210                 215                 220                 


Ala Arg Trp Leu Glu Glu Lys Ile Leu Glu Ile Gly Ala Asp Lys Val 
225                 230                 235                 240 


Ala Ala Phe Val Gly Glu Pro Ile Gln Gly Ala Gly Gly Val Ile Val 
                245                 250                 255     


Pro Pro Ala Thr Tyr Trp Pro Glu Ile Glu Arg Ile Cys Arg Lys Tyr 
            260                 265                 270         


Asp Val Leu Leu Val Ala Asp Glu Val Ile Cys Gly Phe Gly Arg Thr 
        275                 280                 285             


Gly Glu Trp Phe Gly His Gln His Phe Gly Phe Gln Pro Asp Leu Phe 
    290                 295                 300                 


Thr Ala Ala Lys Gly Leu Ser Ser Gly Tyr Leu Pro Ile Gly Ala Val 
305                 310                 315                 320 


Phe Val Gly Lys Arg Val Ala Glu Gly Leu Ile Ala Gly Gly Asp Phe 
                325                 330                 335     


Asn His Gly Phe Thr Tyr Ser Gly His Pro Val Cys Ala Ala Val Ala 
            340                 345                 350         


His Ala Asn Val Ala Ala Leu Arg Asp Glu Gly Ile Val Gln Arg Val 
        355                 360                 365             


Lys Asp Asp Ile Gly Pro Tyr Met Gln Lys Arg Trp Arg Glu Thr Phe 
    370                 375                 380                 


Ser Arg Phe Glu His Val Asp Asp Val Arg Gly Val Gly Met Val Gln 
385                 390                 395                 400 


Ala Phe Thr Leu Val Lys Asn Lys Ala Lys Arg Glu Leu Phe Pro Asp 
                405                 410                 415     


Phe Gly Glu Ile Gly Thr Leu Cys Arg Asp Ile Phe Phe Arg Asn Asn 
            420                 425                 430         


Leu Ile Met Arg Ala Cys Gly Asp His Ile Val Ser Ala Pro Pro Leu 
        435                 440                 445             


Val Met Thr Arg Ala Glu Val Asp Glu Met Leu Ala Val Ala Glu Arg 
    450                 455                 460                 


Cys Leu Glu Glu Phe Glu Gln Thr Leu Lys Ala Arg Gly Leu Ala 
465                 470                 475                 


<210>  8
<211>  36
<212>  DNA
<213>  Artificial

<220>
<223>  Forward Primer

<400>  8
gcgcgcatat gcagaagcaa cgtacgacca gccaat                               36


<210>  9
<211>  37
<212>  DNA
<213>  Artificial

<220>
<223>  Reverse Primer

<400>  9
gatattgtac tcgagctaag ccagcccgcg cgccttc                              37


<210>  10
<211>  1113
<212>  DNA
<213>  Rhodococcus jostii


<220>
<221>  CDS
<222>  (1)..(1113)

<400>  10
atg aag acc aaa gca gcg gtt ctg ttc gaa acg cac aag ccg ttc gaa       48
Met Lys Thr Lys Ala Ala Val Leu Phe Glu Thr His Lys Pro Phe Glu         
1               5                   10                  15              

atc gtg gaa ctc gag ctc gac ggt ccg ggc cct ggc gaa gtg ctc atc       96
Ile Val Glu Leu Glu Leu Asp Gly Pro Gly Pro Gly Glu Val Leu Ile         
            20                  25                  30                  

aag tat gtc gcg gca ggc ctg tgc cac tcg gac ctg cac ctc acc gac      144
Lys Tyr Val Ala Ala Gly Leu Cys His Ser Asp Leu His Leu Thr Asp         
        35                  40                  45                      

ggc gat ctg ccg ccg cgt ttc ccg atc gtc ggc ggg cac gag ggt tcc      192
Gly Asp Leu Pro Pro Arg Phe Pro Ile Val Gly Gly His Glu Gly Ser         
    50                  55                  60                          

ggc atc atc gag gaa gtc ggc ccc ggt gtc acc aag gtg aag ccc ggc      240
Gly Ile Ile Glu Glu Val Gly Pro Gly Val Thr Lys Val Lys Pro Gly         
65                  70                  75                  80          

gac cac gtg gtc tgc agc ttc atc ccg aac tgc ggc acg tgt cgg tac      288
Asp His Val Val Cys Ser Phe Ile Pro Asn Cys Gly Thr Cys Arg Tyr         
                85                  90                  95              

tgt gcc acc ggc cgc cag aac ctg tgt gac atg ggc gcc acc atc ctc      336
Cys Ala Thr Gly Arg Gln Asn Leu Cys Asp Met Gly Ala Thr Ile Leu         
            100                 105                 110                 

gag ggg tcg atg ccc gac ggc tcg ttc cgg ttc cac tcg ggc aag cag      384
Glu Gly Ser Met Pro Asp Gly Ser Phe Arg Phe His Ser Gly Lys Gln         
        115                 120                 125                     

gat ttc ggt gcc atg tgc atg ctc ggt acg ttc gcc gag cgc gcc acc      432
Asp Phe Gly Ala Met Cys Met Leu Gly Thr Phe Ala Glu Arg Ala Thr         
    130                 135                 140                         

atc tcg cag cac tcg gtg gtg aag gtg gac gac tgg ctc ccc ctg gag      480
Ile Ser Gln His Ser Val Val Lys Val Asp Asp Trp Leu Pro Leu Glu         
145                 150                 155                 160         

acc gcc gtc ctc gtc ggc tgc ggt gtg ccg tcc ggt tgg ggc acc gcg      528
Thr Ala Val Leu Val Gly Cys Gly Val Pro Ser Gly Trp Gly Thr Ala         
                165                 170                 175             

gtg tac gcc ggc gga gtt cgc gcc ggt gac acg gtc gtc atc tac ggc      576
Val Tyr Ala Gly Gly Val Arg Ala Gly Asp Thr Val Val Ile Tyr Gly         
            180                 185                 190                 

atc ggc ggt ctc ggc atc aac gcc gtg cag ggc gcg gtc agc tcc ggc      624
Ile Gly Gly Leu Gly Ile Asn Ala Val Gln Gly Ala Val Ser Ser Gly         
        195                 200                 205                     

gcc aag tac gtc gtg gtg gtg gat ccg gtg gcg atg aag cgg gag gcc      672
Ala Lys Tyr Val Val Val Val Asp Pro Val Ala Met Lys Arg Glu Ala         
    210                 215                 220                         

gcg ctg aag ttc ggc gcc acc cac gcg ttc gcg gac gcc gcg acc gcg      720
Ala Leu Lys Phe Gly Ala Thr His Ala Phe Ala Asp Ala Ala Thr Ala         
225                 230                 235                 240         

gcg gag aag gtc aac gaa ctg acc tgg ggt cag ggc gcc gat cag gcc      768
Ala Glu Lys Val Asn Glu Leu Thr Trp Gly Gln Gly Ala Asp Gln Ala         
                245                 250                 255             

ctc atc ctg gtg ggc acc gtc gac gaa gag gtg gtg cag aac gcg acc      816
Leu Ile Leu Val Gly Thr Val Asp Glu Glu Val Val Gln Asn Ala Thr         
            260                 265                 270                 

gcc gtc atc ggc aag ggc ggc acc gtc gtc atc acc ggc ctc gcc gac      864
Ala Val Ile Gly Lys Gly Gly Thr Val Val Ile Thr Gly Leu Ala Asp         
        275                 280                 285                     

ccc gcc aag ctc acc gtg cac atc tcg gga acc gac ctg acc ctg aac      912
Pro Ala Lys Leu Thr Val His Ile Ser Gly Thr Asp Leu Thr Leu Asn         
    290                 295                 300                         

cag aag acg atc aag ggt tcg ctg ttc ggt tcg gcc aac ccg cag tac      960
Gln Lys Thr Ile Lys Gly Ser Leu Phe Gly Ser Ala Asn Pro Gln Tyr         
305                 310                 315                 320         

gac atc gtc cgc ctg ctg cgc ctg tac gac gcg ggt cag ctc aag ctc     1008
Asp Ile Val Arg Leu Leu Arg Leu Tyr Asp Ala Gly Gln Leu Lys Leu         
                325                 330                 335             

gac gag ctg atc acc acc aag tac acg ctc gag cag gtg aac gag ggt     1056
Asp Glu Leu Ile Thr Thr Lys Tyr Thr Leu Glu Gln Val Asn Glu Gly         
            340                 345                 350                 

tac cag gat ctg cgg gac gga aag aac ctg cgt ggc gtg atc gtc cac     1104
Tyr Gln Asp Leu Arg Asp Gly Lys Asn Leu Arg Gly Val Ile Val His         
        355                 360                 365                     

cag cag tag                                                         1113
Gln Gln                                                                 
    370                                                                 


<210>  11
<211>  370
<212>  PRT
<213>  Rhodococcus jostii

<400>  11

Met Lys Thr Lys Ala Ala Val Leu Phe Glu Thr His Lys Pro Phe Glu 
1               5                   10                  15      


Ile Val Glu Leu Glu Leu Asp Gly Pro Gly Pro Gly Glu Val Leu Ile 
            20                  25                  30          


Lys Tyr Val Ala Ala Gly Leu Cys His Ser Asp Leu His Leu Thr Asp 
        35                  40                  45              


Gly Asp Leu Pro Pro Arg Phe Pro Ile Val Gly Gly His Glu Gly Ser 
    50                  55                  60                  


Gly Ile Ile Glu Glu Val Gly Pro Gly Val Thr Lys Val Lys Pro Gly 
65                  70                  75                  80  


Asp His Val Val Cys Ser Phe Ile Pro Asn Cys Gly Thr Cys Arg Tyr 
                85                  90                  95      


Cys Ala Thr Gly Arg Gln Asn Leu Cys Asp Met Gly Ala Thr Ile Leu 
            100                 105                 110         


Glu Gly Ser Met Pro Asp Gly Ser Phe Arg Phe His Ser Gly Lys Gln 
        115                 120                 125             


Asp Phe Gly Ala Met Cys Met Leu Gly Thr Phe Ala Glu Arg Ala Thr 
    130                 135                 140                 


Ile Ser Gln His Ser Val Val Lys Val Asp Asp Trp Leu Pro Leu Glu 
145                 150                 155                 160 


Thr Ala Val Leu Val Gly Cys Gly Val Pro Ser Gly Trp Gly Thr Ala 
                165                 170                 175     


Val Tyr Ala Gly Gly Val Arg Ala Gly Asp Thr Val Val Ile Tyr Gly 
            180                 185                 190         


Ile Gly Gly Leu Gly Ile Asn Ala Val Gln Gly Ala Val Ser Ser Gly 
        195                 200                 205             


Ala Lys Tyr Val Val Val Val Asp Pro Val Ala Met Lys Arg Glu Ala 
    210                 215                 220                 


Ala Leu Lys Phe Gly Ala Thr His Ala Phe Ala Asp Ala Ala Thr Ala 
225                 230                 235                 240 


Ala Glu Lys Val Asn Glu Leu Thr Trp Gly Gln Gly Ala Asp Gln Ala 
                245                 250                 255     


Leu Ile Leu Val Gly Thr Val Asp Glu Glu Val Val Gln Asn Ala Thr 
            260                 265                 270         


Ala Val Ile Gly Lys Gly Gly Thr Val Val Ile Thr Gly Leu Ala Asp 
        275                 280                 285             


Pro Ala Lys Leu Thr Val His Ile Ser Gly Thr Asp Leu Thr Leu Asn 
    290                 295                 300                 


Gln Lys Thr Ile Lys Gly Ser Leu Phe Gly Ser Ala Asn Pro Gln Tyr 
305                 310                 315                 320 


Asp Ile Val Arg Leu Leu Arg Leu Tyr Asp Ala Gly Gln Leu Lys Leu 
                325                 330                 335     


Asp Glu Leu Ile Thr Thr Lys Tyr Thr Leu Glu Gln Val Asn Glu Gly 
            340                 345                 350         


Tyr Gln Asp Leu Arg Asp Gly Lys Asn Leu Arg Gly Val Ile Val His 
        355                 360                 365             


Gln Gln 
    370 


<210>  12
<211>  390
<212>  PRT
<213>  Artificial

<220>
<223>  Rj_ADH with His Tag

<400>  12

Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His Met Lys Thr Lys Ala Ala Val Leu Phe Glu Thr His 
            20                  25                  30          


Lys Pro Phe Glu Ile Val Glu Leu Glu Leu Asp Gly Pro Gly Pro Gly 
        35                  40                  45              


Glu Val Leu Ile Lys Tyr Val Ala Ala Gly Leu Cys His Ser Asp Leu 
    50                  55                  60                  


His Leu Thr Asp Gly Asp Leu Pro Pro Arg Phe Pro Ile Val Gly Gly 
65                  70                  75                  80  


His Glu Gly Ser Gly Ile Ile Glu Glu Val Gly Pro Gly Val Thr Lys 
                85                  90                  95      


Val Lys Pro Gly Asp His Val Val Cys Ser Phe Ile Pro Asn Cys Gly 
            100                 105                 110         


Thr Cys Arg Tyr Cys Ala Thr Gly Arg Gln Asn Leu Cys Asp Met Gly 
        115                 120                 125             


Ala Thr Ile Leu Glu Gly Ser Met Pro Asp Gly Ser Phe Arg Phe His 
    130                 135                 140                 


Ser Gly Lys Gln Asp Phe Gly Ala Met Cys Met Leu Gly Thr Phe Ala 
145                 150                 155                 160 


Glu Arg Ala Thr Ile Ser Gln His Ser Val Val Lys Val Asp Asp Trp 
                165                 170                 175     


Leu Pro Leu Glu Thr Ala Val Leu Val Gly Cys Gly Val Pro Ser Gly 
            180                 185                 190         


Trp Gly Thr Ala Val Tyr Ala Gly Gly Val Arg Ala Gly Asp Thr Val 
        195                 200                 205             


Val Ile Tyr Gly Ile Gly Gly Leu Gly Ile Asn Ala Val Gln Gly Ala 
    210                 215                 220                 


Val Ser Ser Gly Ala Lys Tyr Val Val Val Val Asp Pro Val Ala Met 
225                 230                 235                 240 


Lys Arg Glu Ala Ala Leu Lys Phe Gly Ala Thr His Ala Phe Ala Asp 
                245                 250                 255     


Ala Ala Thr Ala Ala Glu Lys Val Asn Glu Leu Thr Trp Gly Gln Gly 
            260                 265                 270         


Ala Asp Gln Ala Leu Ile Leu Val Gly Thr Val Asp Glu Glu Val Val 
        275                 280                 285             


Gln Asn Ala Thr Ala Val Ile Gly Lys Gly Gly Thr Val Val Ile Thr 
    290                 295                 300                 


Gly Leu Ala Asp Pro Ala Lys Leu Thr Val His Ile Ser Gly Thr Asp 
305                 310                 315                 320 


Leu Thr Leu Asn Gln Lys Thr Ile Lys Gly Ser Leu Phe Gly Ser Ala 
                325                 330                 335     


Asn Pro Gln Tyr Asp Ile Val Arg Leu Leu Arg Leu Tyr Asp Ala Gly 
            340                 345                 350         


Gln Leu Lys Leu Asp Glu Leu Ile Thr Thr Lys Tyr Thr Leu Glu Gln 
        355                 360                 365             


Val Asn Glu Gly Tyr Gln Asp Leu Arg Asp Gly Lys Asn Leu Arg Gly 
    370                 375                 380                 


Val Ile Val His Gln Gln 
385                 390 


<210>  13
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Forward Primer

<400>  13
gcgcgcatat gaagaccaaa gcagcggttc tg                                   32


<210>  14
<211>  33
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Reverse Primer

<400>  14
gagctaagct tctactgctg gtggacgatc acg                                  33


<210>  15
<211>  1014
<212>  DNA
<213>  Geobacillus stearothermophilus


<220>
<221>  CDS
<222>  (1)..(1014)

<400>  15
atg aaa gct gca gtt gtg gaa caa ttt aaa aag ccg tta caa gtg aaa       48
Met Lys Ala Ala Val Val Glu Gln Phe Lys Lys Pro Leu Gln Val Lys         
1               5                   10                  15              

gaa gtg gaa aaa cct aag atc tca tac ggg gaa gta tta gtg cgc atc       96
Glu Val Glu Lys Pro Lys Ile Ser Tyr Gly Glu Val Leu Val Arg Ile         
            20                  25                  30                  

aaa gcg tgt ggg gta tgc cat aca gac ttg cat gcc gca cat ggc gac      144
Lys Ala Cys Gly Val Cys His Thr Asp Leu His Ala Ala His Gly Asp         
        35                  40                  45                      

tgg cct gta aag cct aaa ctg cct ctc att cct ggc cat gaa ggc gtc      192
Trp Pro Val Lys Pro Lys Leu Pro Leu Ile Pro Gly His Glu Gly Val         
    50                  55                  60                          

ggt gta att gaa gaa gta ggt cct ggg gta aca cat tta aaa gtt gga      240
Gly Val Ile Glu Glu Val Gly Pro Gly Val Thr His Leu Lys Val Gly         
65                  70                  75                  80          

gat cgc gta ggt atc cct tgg ctt tat tcg gcg tgc ggt cat tgt gac      288
Asp Arg Val Gly Ile Pro Trp Leu Tyr Ser Ala Cys Gly His Cys Asp         
                85                  90                  95              

tat tgc tta agc gga caa gaa aca tta tgc gaa cgt caa caa aac gct      336
Tyr Cys Leu Ser Gly Gln Glu Thr Leu Cys Glu Arg Gln Gln Asn Ala         
            100                 105                 110                 

ggc tat tcc gtc gat ggt ggt tat gct gaa tat tgc cgt gct gca gcc      384
Gly Tyr Ser Val Asp Gly Gly Tyr Ala Glu Tyr Cys Arg Ala Ala Ala         
        115                 120                 125                     

gat tat gtc gta aaa att cct gat aac tta tcg ttt gaa gaa gcc gct      432
Asp Tyr Val Val Lys Ile Pro Asp Asn Leu Ser Phe Glu Glu Ala Ala         
    130                 135                 140                         

cca atc ttt tgc gct ggt gta aca aca tat aaa gcg ctc aaa gta aca      480
Pro Ile Phe Cys Ala Gly Val Thr Thr Tyr Lys Ala Leu Lys Val Thr         
145                 150                 155                 160         

ggc gca aaa cca ggt gaa tgg gta gcc att tac ggt atc ggc ggg ctt      528
Gly Ala Lys Pro Gly Glu Trp Val Ala Ile Tyr Gly Ile Gly Gly Leu         
                165                 170                 175             

gga cat gtc gca gtc caa tac gca aag gcg atg ggg tta aac gtc gtt      576
Gly His Val Ala Val Gln Tyr Ala Lys Ala Met Gly Leu Asn Val Val         
            180                 185                 190                 

gct gtc gat tta ggt gat gaa aaa ctt gag ctt gct aaa caa ctt ggt      624
Ala Val Asp Leu Gly Asp Glu Lys Leu Glu Leu Ala Lys Gln Leu Gly         
        195                 200                 205                     

gca gat ctt gtc gtc aat ccg aaa cat gat gat gca gca caa tgg ata      672
Ala Asp Leu Val Val Asn Pro Lys His Asp Asp Ala Ala Gln Trp Ile         
    210                 215                 220                         

aaa gaa aaa gtg ggc ggt gtg cat gcg act gtc gtc aca gct gtt tca      720
Lys Glu Lys Val Gly Gly Val His Ala Thr Val Val Thr Ala Val Ser         
225                 230                 235                 240         

aaa gcc gcg ttc gaa tca gcc tac aaa tcc att cgt cgc ggt ggt gct      768
Lys Ala Ala Phe Glu Ser Ala Tyr Lys Ser Ile Arg Arg Gly Gly Ala         
                245                 250                 255             

tgc gta ctc gtc gga tta ccg ccg gaa gaa ata cct att cca att ttc      816
Cys Val Leu Val Gly Leu Pro Pro Glu Glu Ile Pro Ile Pro Ile Phe         
            260                 265                 270                 

gat aca gta tta aat gga gta aaa att att ggt tct atc gtt ggt acg      864
Asp Thr Val Leu Asn Gly Val Lys Ile Ile Gly Ser Ile Val Gly Thr         
        275                 280                 285                     

cgc aaa gac tta caa gag gca ctt caa ttt gca gca gaa gga aaa gta      912
Arg Lys Asp Leu Gln Glu Ala Leu Gln Phe Ala Ala Glu Gly Lys Val         
    290                 295                 300                         

aaa aca att gtc gaa gtg caa ccg ctt gaa aac att aac gac gta ttc      960
Lys Thr Ile Val Glu Val Gln Pro Leu Glu Asn Ile Asn Asp Val Phe         
305                 310                 315                 320         

gat cgt atg tta aaa ggg caa att aac ggc cgc gtc gtg tta aaa gta     1008
Asp Arg Met Leu Lys Gly Gln Ile Asn Gly Arg Val Val Leu Lys Val         
                325                 330                 335             

gat taa                                                             1014
Asp                                                                     
                                                                        


<210>  16
<211>  337
<212>  PRT
<213>  Geobacillus stearothermophilus

<400>  16

Met Lys Ala Ala Val Val Glu Gln Phe Lys Lys Pro Leu Gln Val Lys 
1               5                   10                  15      


Glu Val Glu Lys Pro Lys Ile Ser Tyr Gly Glu Val Leu Val Arg Ile 
            20                  25                  30          


Lys Ala Cys Gly Val Cys His Thr Asp Leu His Ala Ala His Gly Asp 
        35                  40                  45              


Trp Pro Val Lys Pro Lys Leu Pro Leu Ile Pro Gly His Glu Gly Val 
    50                  55                  60                  


Gly Val Ile Glu Glu Val Gly Pro Gly Val Thr His Leu Lys Val Gly 
65                  70                  75                  80  


Asp Arg Val Gly Ile Pro Trp Leu Tyr Ser Ala Cys Gly His Cys Asp 
                85                  90                  95      


Tyr Cys Leu Ser Gly Gln Glu Thr Leu Cys Glu Arg Gln Gln Asn Ala 
            100                 105                 110         


Gly Tyr Ser Val Asp Gly Gly Tyr Ala Glu Tyr Cys Arg Ala Ala Ala 
        115                 120                 125             


Asp Tyr Val Val Lys Ile Pro Asp Asn Leu Ser Phe Glu Glu Ala Ala 
    130                 135                 140                 


Pro Ile Phe Cys Ala Gly Val Thr Thr Tyr Lys Ala Leu Lys Val Thr 
145                 150                 155                 160 


Gly Ala Lys Pro Gly Glu Trp Val Ala Ile Tyr Gly Ile Gly Gly Leu 
                165                 170                 175     


Gly His Val Ala Val Gln Tyr Ala Lys Ala Met Gly Leu Asn Val Val 
            180                 185                 190         


Ala Val Asp Leu Gly Asp Glu Lys Leu Glu Leu Ala Lys Gln Leu Gly 
        195                 200                 205             


Ala Asp Leu Val Val Asn Pro Lys His Asp Asp Ala Ala Gln Trp Ile 
    210                 215                 220                 


Lys Glu Lys Val Gly Gly Val His Ala Thr Val Val Thr Ala Val Ser 
225                 230                 235                 240 


Lys Ala Ala Phe Glu Ser Ala Tyr Lys Ser Ile Arg Arg Gly Gly Ala 
                245                 250                 255     


Cys Val Leu Val Gly Leu Pro Pro Glu Glu Ile Pro Ile Pro Ile Phe 
            260                 265                 270         


Asp Thr Val Leu Asn Gly Val Lys Ile Ile Gly Ser Ile Val Gly Thr 
        275                 280                 285             


Arg Lys Asp Leu Gln Glu Ala Leu Gln Phe Ala Ala Glu Gly Lys Val 
    290                 295                 300                 


Lys Thr Ile Val Glu Val Gln Pro Leu Glu Asn Ile Asn Asp Val Phe 
305                 310                 315                 320 


Asp Arg Met Leu Lys Gly Gln Ile Asn Gly Arg Val Val Leu Lys Val 
                325                 330                 335     


Asp 
    


<210>  17
<211>  357
<212>  PRT
<213>  Artificial

<220>
<223>  Gs_ADH1 with His Tag

<400>  17

Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His Met Lys Ala Ala Val Val Glu Gln Phe Lys Lys Pro 
            20                  25                  30          


Leu Gln Val Lys Glu Val Glu Lys Pro Lys Ile Ser Tyr Gly Glu Val 
        35                  40                  45              


Leu Val Arg Ile Lys Ala Cys Gly Val Cys His Thr Asp Leu His Ala 
    50                  55                  60                  


Ala His Gly Asp Trp Pro Val Lys Pro Lys Leu Pro Leu Ile Pro Gly 
65                  70                  75                  80  


His Glu Gly Val Gly Val Ile Glu Glu Val Gly Pro Gly Val Thr His 
                85                  90                  95      


Leu Lys Val Gly Asp Arg Val Gly Ile Pro Trp Leu Tyr Ser Ala Cys 
            100                 105                 110         


Gly His Cys Asp Tyr Cys Leu Ser Gly Gln Glu Thr Leu Cys Glu Arg 
        115                 120                 125             


Gln Gln Asn Ala Gly Tyr Ser Val Asp Gly Gly Tyr Ala Glu Tyr Cys 
    130                 135                 140                 


Arg Ala Ala Ala Asp Tyr Val Val Lys Ile Pro Asp Asn Leu Ser Phe 
145                 150                 155                 160 


Glu Glu Ala Ala Pro Ile Phe Cys Ala Gly Val Thr Thr Tyr Lys Ala 
                165                 170                 175     


Leu Lys Val Thr Gly Ala Lys Pro Gly Glu Trp Val Ala Ile Tyr Gly 
            180                 185                 190         


Ile Gly Gly Leu Gly His Val Ala Val Gln Tyr Ala Lys Ala Met Gly 
        195                 200                 205             


Leu Asn Val Val Ala Val Asp Leu Gly Asp Glu Lys Leu Glu Leu Ala 
    210                 215                 220                 


Lys Gln Leu Gly Ala Asp Leu Val Val Asn Pro Lys His Asp Asp Ala 
225                 230                 235                 240 


Ala Gln Trp Ile Lys Glu Lys Val Gly Gly Val His Ala Thr Val Val 
                245                 250                 255     


Thr Ala Val Ser Lys Ala Ala Phe Glu Ser Ala Tyr Lys Ser Ile Arg 
            260                 265                 270         


Arg Gly Gly Ala Cys Val Leu Val Gly Leu Pro Pro Glu Glu Ile Pro 
        275                 280                 285             


Ile Pro Ile Phe Asp Thr Val Leu Asn Gly Val Lys Ile Ile Gly Ser 
    290                 295                 300                 


Ile Val Gly Thr Arg Lys Asp Leu Gln Glu Ala Leu Gln Phe Ala Ala 
305                 310                 315                 320 


Glu Gly Lys Val Lys Thr Ile Val Glu Val Gln Pro Leu Glu Asn Ile 
                325                 330                 335     


Asn Asp Val Phe Asp Arg Met Leu Lys Gly Gln Ile Asn Gly Arg Val 
            340                 345                 350         


Val Leu Lys Val Asp 
        355         


<210>  18
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Forward Primer

<400>  18
gcttgaccat atgaaagctg cagttgtgga ac                                   32


<210>  19
<211>  33
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Reverse Primer

<400>  19
ggatcgctcg agttaatcta cttttaacac gac                                  33


<210>  20
<211>  1020
<212>  DNA
<213>  Geobacillus stearothermophilus


<220>
<221>  CDS
<222>  (1)..(1020)

<400>  20
atg aaa gca gca gta gtt aac gaa ttt aaa aaa gca ctt gaa att aaa       48
Met Lys Ala Ala Val Val Asn Glu Phe Lys Lys Ala Leu Glu Ile Lys         
1               5                   10                  15              

gag gtg gaa aga ccc aaa cta gag gaa ggt gaa gtt tta gtt aag atc       96
Glu Val Glu Arg Pro Lys Leu Glu Glu Gly Glu Val Leu Val Lys Ile         
            20                  25                  30                  

gaa gca tgc ggt gtg tgc cat acg gat tta cat gca gcc cat ggt gac      144
Glu Ala Cys Gly Val Cys His Thr Asp Leu His Ala Ala His Gly Asp         
        35                  40                  45                      

tgg ccg att aaa ccg aaa ttg ccg tta att ccg ggg cat gaa gga gtc      192
Trp Pro Ile Lys Pro Lys Leu Pro Leu Ile Pro Gly His Glu Gly Val         
    50                  55                  60                          

gga att gtt gta gaa gtc gca aag gga gtt aaa tca ata aaa gtg gga      240
Gly Ile Val Val Glu Val Ala Lys Gly Val Lys Ser Ile Lys Val Gly         
65                  70                  75                  80          

gat cgc gtc ggc atc cca tgg tta tat tct gca tgt ggt gaa tgt gaa      288
Asp Arg Val Gly Ile Pro Trp Leu Tyr Ser Ala Cys Gly Glu Cys Glu         
                85                  90                  95              

tat tgt tta act ggt caa gaa aca ctt tgc ccg cat caa ttg aat ggc      336
Tyr Cys Leu Thr Gly Gln Glu Thr Leu Cys Pro His Gln Leu Asn Gly         
            100                 105                 110                 

gga tat tcc gtt gat ggc gga tat gcg gaa tac tgt aaa gct ccc gca      384
Gly Tyr Ser Val Asp Gly Gly Tyr Ala Glu Tyr Cys Lys Ala Pro Ala         
        115                 120                 125                     

gat tat gtg gcg aaa att cct gat aac tta gat cca gtt gaa gta gcg      432
Asp Tyr Val Ala Lys Ile Pro Asp Asn Leu Asp Pro Val Glu Val Ala         
    130                 135                 140                         

cca att tta tgt gca ggc gta acg aca tat aaa gcg ttg aaa gta tca      480
Pro Ile Leu Cys Ala Gly Val Thr Thr Tyr Lys Ala Leu Lys Val Ser         
145                 150                 155                 160         

ggt gct cgg cca ggt gaa tgg gtg gcc att tac ggt att gga gga ctt      528
Gly Ala Arg Pro Gly Glu Trp Val Ala Ile Tyr Gly Ile Gly Gly Leu         
                165                 170                 175             

ggt cat att gca tta caa tac gcc aaa gcg atg gga ctt aat gtg gta      576
Gly His Ile Ala Leu Gln Tyr Ala Lys Ala Met Gly Leu Asn Val Val         
            180                 185                 190                 

gct gtt gat atc agt gat gaa aaa tca aaa tta gcc aaa gat tta ggt      624
Ala Val Asp Ile Ser Asp Glu Lys Ser Lys Leu Ala Lys Asp Leu Gly         
        195                 200                 205                     

gca gat att gca atc aac ggt tta aaa gaa gat cct gtc aaa gcg ata      672
Ala Asp Ile Ala Ile Asn Gly Leu Lys Glu Asp Pro Val Lys Ala Ile         
    210                 215                 220                         

cat gat caa gta ggc gga gtt cat gct gct att agt gta gcg gtg aat      720
His Asp Gln Val Gly Gly Val His Ala Ala Ile Ser Val Ala Val Asn         
225                 230                 235                 240         

aaa aaa gca ttt gaa cag gcc tat cag tca gtg aaa cgg gga ggc aca      768
Lys Lys Ala Phe Glu Gln Ala Tyr Gln Ser Val Lys Arg Gly Gly Thr         
                245                 250                 255             

tta gtt gtt gtc ggt ctg cca aat gct gat ctg cct att ccg atc ttc      816
Leu Val Val Val Gly Leu Pro Asn Ala Asp Leu Pro Ile Pro Ile Phe         
            260                 265                 270                 

gat aca gta tta aac ggc gtt tcc gtt aaa ggt tcc atc gtc ggt aca      864
Asp Thr Val Leu Asn Gly Val Ser Val Lys Gly Ser Ile Val Gly Thr         
        275                 280                 285                     

aga aag gac atg cag gaa gca tta gat ttt gct gct cgc gga aaa gtc      912
Arg Lys Asp Met Gln Glu Ala Leu Asp Phe Ala Ala Arg Gly Lys Val         
    290                 295                 300                         

cgt cca att gtt gaa acc gct gaa tta gaa gaa ata aat gaa gta ttt      960
Arg Pro Ile Val Glu Thr Ala Glu Leu Glu Glu Ile Asn Glu Val Phe         
305                 310                 315                 320         

gaa aga atg gaa aaa gga aaa atc aac ggt cgt atc gta cta aaa ttg     1008
Glu Arg Met Glu Lys Gly Lys Ile Asn Gly Arg Ile Val Leu Lys Leu         
                325                 330                 335             

aag gag gat tga                                                     1020
Lys Glu Asp                                                             
                                                                        


<210>  21
<211>  339
<212>  PRT
<213>  Geobacillus stearothermophilus

<400>  21

Met Lys Ala Ala Val Val Asn Glu Phe Lys Lys Ala Leu Glu Ile Lys 
1               5                   10                  15      


Glu Val Glu Arg Pro Lys Leu Glu Glu Gly Glu Val Leu Val Lys Ile 
            20                  25                  30          


Glu Ala Cys Gly Val Cys His Thr Asp Leu His Ala Ala His Gly Asp 
        35                  40                  45              


Trp Pro Ile Lys Pro Lys Leu Pro Leu Ile Pro Gly His Glu Gly Val 
    50                  55                  60                  


Gly Ile Val Val Glu Val Ala Lys Gly Val Lys Ser Ile Lys Val Gly 
65                  70                  75                  80  


Asp Arg Val Gly Ile Pro Trp Leu Tyr Ser Ala Cys Gly Glu Cys Glu 
                85                  90                  95      


Tyr Cys Leu Thr Gly Gln Glu Thr Leu Cys Pro His Gln Leu Asn Gly 
            100                 105                 110         


Gly Tyr Ser Val Asp Gly Gly Tyr Ala Glu Tyr Cys Lys Ala Pro Ala 
        115                 120                 125             


Asp Tyr Val Ala Lys Ile Pro Asp Asn Leu Asp Pro Val Glu Val Ala 
    130                 135                 140                 


Pro Ile Leu Cys Ala Gly Val Thr Thr Tyr Lys Ala Leu Lys Val Ser 
145                 150                 155                 160 


Gly Ala Arg Pro Gly Glu Trp Val Ala Ile Tyr Gly Ile Gly Gly Leu 
                165                 170                 175     


Gly His Ile Ala Leu Gln Tyr Ala Lys Ala Met Gly Leu Asn Val Val 
            180                 185                 190         


Ala Val Asp Ile Ser Asp Glu Lys Ser Lys Leu Ala Lys Asp Leu Gly 
        195                 200                 205             


Ala Asp Ile Ala Ile Asn Gly Leu Lys Glu Asp Pro Val Lys Ala Ile 
    210                 215                 220                 


His Asp Gln Val Gly Gly Val His Ala Ala Ile Ser Val Ala Val Asn 
225                 230                 235                 240 


Lys Lys Ala Phe Glu Gln Ala Tyr Gln Ser Val Lys Arg Gly Gly Thr 
                245                 250                 255     


Leu Val Val Val Gly Leu Pro Asn Ala Asp Leu Pro Ile Pro Ile Phe 
            260                 265                 270         


Asp Thr Val Leu Asn Gly Val Ser Val Lys Gly Ser Ile Val Gly Thr 
        275                 280                 285             


Arg Lys Asp Met Gln Glu Ala Leu Asp Phe Ala Ala Arg Gly Lys Val 
    290                 295                 300                 


Arg Pro Ile Val Glu Thr Ala Glu Leu Glu Glu Ile Asn Glu Val Phe 
305                 310                 315                 320 


Glu Arg Met Glu Lys Gly Lys Ile Asn Gly Arg Ile Val Leu Lys Leu 
                325                 330                 335     


Lys Glu Asp 
            


<210>  22
<211>  359
<212>  PRT
<213>  Artificial

<220>
<223>  Gs_ADH2 with His Tag

<400>  22

Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His Met Lys Ala Ala Val Val Asn Glu Phe Lys Lys Ala 
            20                  25                  30          


Leu Glu Ile Lys Glu Val Glu Arg Pro Lys Leu Glu Glu Gly Glu Val 
        35                  40                  45              


Leu Val Lys Ile Glu Ala Cys Gly Val Cys His Thr Asp Leu His Ala 
    50                  55                  60                  


Ala His Gly Asp Trp Pro Ile Lys Pro Lys Leu Pro Leu Ile Pro Gly 
65                  70                  75                  80  


His Glu Gly Val Gly Ile Val Val Glu Val Ala Lys Gly Val Lys Ser 
                85                  90                  95      


Ile Lys Val Gly Asp Arg Val Gly Ile Pro Trp Leu Tyr Ser Ala Cys 
            100                 105                 110         


Gly Glu Cys Glu Tyr Cys Leu Thr Gly Gln Glu Thr Leu Cys Pro His 
        115                 120                 125             


Gln Leu Asn Gly Gly Tyr Ser Val Asp Gly Gly Tyr Ala Glu Tyr Cys 
    130                 135                 140                 


Lys Ala Pro Ala Asp Tyr Val Ala Lys Ile Pro Asp Asn Leu Asp Pro 
145                 150                 155                 160 


Val Glu Val Ala Pro Ile Leu Cys Ala Gly Val Thr Thr Tyr Lys Ala 
                165                 170                 175     


Leu Lys Val Ser Gly Ala Arg Pro Gly Glu Trp Val Ala Ile Tyr Gly 
            180                 185                 190         


Ile Gly Gly Leu Gly His Ile Ala Leu Gln Tyr Ala Lys Ala Met Gly 
        195                 200                 205             


Leu Asn Val Val Ala Val Asp Ile Ser Asp Glu Lys Ser Lys Leu Ala 
    210                 215                 220                 


Lys Asp Leu Gly Ala Asp Ile Ala Ile Asn Gly Leu Lys Glu Asp Pro 
225                 230                 235                 240 


Val Lys Ala Ile His Asp Gln Val Gly Gly Val His Ala Ala Ile Ser 
                245                 250                 255     


Val Ala Val Asn Lys Lys Ala Phe Glu Gln Ala Tyr Gln Ser Val Lys 
            260                 265                 270         


Arg Gly Gly Thr Leu Val Val Val Gly Leu Pro Asn Ala Asp Leu Pro 
        275                 280                 285             


Ile Pro Ile Phe Asp Thr Val Leu Asn Gly Val Ser Val Lys Gly Ser 
    290                 295                 300                 


Ile Val Gly Thr Arg Lys Asp Met Gln Glu Ala Leu Asp Phe Ala Ala 
305                 310                 315                 320 


Arg Gly Lys Val Arg Pro Ile Val Glu Thr Ala Glu Leu Glu Glu Ile 
                325                 330                 335     


Asn Glu Val Phe Glu Arg Met Glu Lys Gly Lys Ile Asn Gly Arg Ile 
            340                 345                 350         


Val Leu Lys Leu Lys Glu Asp 
        355                 


<210>  23
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Forward Primer

<400>  23
gcttgaccat atgaaagcag cagtagttaa cg                                   32


<210>  24
<211>  37
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Reverse Primer

<400>  24
ggatcgctcg agtcaatcct ccttcaattt tagtacg                              37


<210>  25
<211>  1011
<212>  DNA
<213>  Pseudomonas putida

<400>  25
atgaaagctg ctgtcgttgc accaggccgt cgcgtggacg tggtagagaa aaccctgcgc     60

cctctcgaac acggcgaagc gctgctgaag atgcagtgct gcggtgtgtg ccacaccgac    120

ctgcacgtga aaaatggcga ctttggtgac aagaccggtg tagtactggg ccacgaaggt    180

attggcgtgg tccaggaagt tggcccgggc gtcacctcgc tcaagccagg cgaccgtgcc    240

agcgttgcct ggttctacca gggttgcgga cattgcgaat attgcaacag cggcaacgag    300

accctgtgcc gcgacgtgaa gaattccggc tacaccgtgg acggcggcat ggccgaggcc    360

tgcatcgtca aggccgacta ctcggttaaa gtgcccgacg gcctcgactc cgctgccgcc    420

agcagcatca cctgcgccgg cgtcacgacc tacaaggcag tgaaaatctc caacgtccgc    480

cccggccaat ggatcgccat ctacgggctc ggcggcctgg gcaacctggc cctgcaatat    540

gccaagaatg tgttcaacgc caaagtgatc gccatcgacg tcaacgaaga gcaactgcgc    600

ttcgccagcg agatgggcgc ggatctggtc gtcaatccgc tcaacgaaga cgccgctaag    660

ttcatccagg ccaagaccgg tggcgcacat gctgccgtcg ttactgccgt ggccaaaggc    720

gcgttcaatt ctgccgtcga tgccttgcgc gctggcgggc gactggtggc cgtcgggctt    780

ccgtcggagt ccatggacct gaatattcct cgcctggtgc ttgatgggat cgaagtggtg    840

ggctcgctgg taggtacacg ccaggacctg caggaagcct tccagtttgc cgctgaaggc    900

aaagtggtgc ccaaggtaac gctgcgaccg atcgagcata tcaaccagat ctttgacgag    960

atgctggagg gcaagatcaa aggccggatg gtgatacagt tcgaaggctg a            1011


<210>  26
<211>  1017
<212>  DNA
<213>  Artificial

<220>
<223>  Pp_ADH NS codon optimized


<220>
<221>  CDS
<222>  (1)..(1017)

<400>  26
atg aaa gca gcg gtg gtc gca cct ggt cgt cgt gtg gac gta gtc gag       48
Met Lys Ala Ala Val Val Ala Pro Gly Arg Arg Val Asp Val Val Glu         
1               5                   10                  15              

aaa acc ctg cgt ccg ctg gaa cat ggt gag gcg ctg ctg aag atg caa       96
Lys Thr Leu Arg Pro Leu Glu His Gly Glu Ala Leu Leu Lys Met Gln         
            20                  25                  30                  

tgt tgt ggt gtc tgc cac acg gat ttg cac gtg aag aac ggc gac ttt      144
Cys Cys Gly Val Cys His Thr Asp Leu His Val Lys Asn Gly Asp Phe         
        35                  40                  45                      

ggt gat aag acc ggt gtt gtc ctg ggc cat gaa ggc atc ggc gtg gtt      192
Gly Asp Lys Thr Gly Val Val Leu Gly His Glu Gly Ile Gly Val Val         
    50                  55                  60                          

caa gag gtc ggt ccg ggt gtt acc agc ttg aaa ccg ggc gac cgt gca      240
Gln Glu Val Gly Pro Gly Val Thr Ser Leu Lys Pro Gly Asp Arg Ala         
65                  70                  75                  80          

agc gtc gcg tgg ttc tat cag ggc tgc ggc cac tgt gag tat tgc aac      288
Ser Val Ala Trp Phe Tyr Gln Gly Cys Gly His Cys Glu Tyr Cys Asn         
                85                  90                  95              

tcg ggt aat gaa act ttg tgc cgc gat gtg aaa aac agc ggt tac acg      336
Ser Gly Asn Glu Thr Leu Cys Arg Asp Val Lys Asn Ser Gly Tyr Thr         
            100                 105                 110                 

gtt gac ggt ggc atg gcc gaa gcg tgc att gtc aag gcg gac tac agc      384
Val Asp Gly Gly Met Ala Glu Ala Cys Ile Val Lys Ala Asp Tyr Ser         
        115                 120                 125                     

gtt aaa gtg cca gat ggt ctg gac agc gca gct gcc tcc agc att acc      432
Val Lys Val Pro Asp Gly Leu Asp Ser Ala Ala Ala Ser Ser Ile Thr         
    130                 135                 140                         

tgt gcg ggc gtg acc acc tac aaa gcg gtg aag atc agc aat gtt cgt      480
Cys Ala Gly Val Thr Thr Tyr Lys Ala Val Lys Ile Ser Asn Val Arg         
145                 150                 155                 160         

cga tcg ccg ggt cag tgg att gct att tac ggt ctg ggt ggt ctg ggc      528
Arg Ser Pro Gly Gln Trp Ile Ala Ile Tyr Gly Leu Gly Gly Leu Gly         
                165                 170                 175             

aac ctg gca ctg caa tat gcg aag aat gtg ttc aat gcg aag gtt atc      576
Asn Leu Ala Leu Gln Tyr Ala Lys Asn Val Phe Asn Ala Lys Val Ile         
            180                 185                 190                 

gcg att gat gtc aat gaa gag cag ttg cgt ttc gcc agc gaa atg ggt      624
Ala Ile Asp Val Asn Glu Glu Gln Leu Arg Phe Ala Ser Glu Met Gly         
        195                 200                 205                     

gcg gat ctg gtt gtt aac ccg ctg aac gag gac gcc gca aag ttt atc      672
Ala Asp Leu Val Val Asn Pro Leu Asn Glu Asp Ala Ala Lys Phe Ile         
    210                 215                 220                         

cag gca aaa acg ggt ggc gcg cat gcc gcg gta gtg acc gcg gtt gcg      720
Gln Ala Lys Thr Gly Gly Ala His Ala Ala Val Val Thr Ala Val Ala         
225                 230                 235                 240         

aaa ggc gca ttc aat tcc gcc gtg gac gcc ttg cgc gct ggc ggt cgc      768
Lys Gly Ala Phe Asn Ser Ala Val Asp Ala Leu Arg Ala Gly Gly Arg         
                245                 250                 255             

ctg gtt gca gtt ggc ctg ccg agc gag tct atg gat ctg aat atc ccg      816
Leu Val Ala Val Gly Leu Pro Ser Glu Ser Met Asp Leu Asn Ile Pro         
            260                 265                 270                 

cgt ctg gtt ctg gac ggt att gag gtc gtc ggc agc ctg gtg ggt acc      864
Arg Leu Val Leu Asp Gly Ile Glu Val Val Gly Ser Leu Val Gly Thr         
        275                 280                 285                     

cgc cag gat ctg cag gaa gcg ttt cag ttt gcg gct gaa ggt aaa gtc      912
Arg Gln Asp Leu Gln Glu Ala Phe Gln Phe Ala Ala Glu Gly Lys Val         
    290                 295                 300                         

gtg ccg aag gtt acg ctg cgt ccg att gag cac att aac caa atc ttt      960
Val Pro Lys Val Thr Leu Arg Pro Ile Glu His Ile Asn Gln Ile Phe         
305                 310                 315                 320         

gac gag atg ctg gaa ggt aag atc aaa ggt cgc atg gtg atc caa ttc     1008
Asp Glu Met Leu Glu Gly Lys Ile Lys Gly Arg Met Val Ile Gln Phe         
                325                 330                 335             

gag ggt taa                                                         1017
Glu Gly                                                                 
                                                                        


<210>  27
<211>  338
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  27

Met Lys Ala Ala Val Val Ala Pro Gly Arg Arg Val Asp Val Val Glu 
1               5                   10                  15      


Lys Thr Leu Arg Pro Leu Glu His Gly Glu Ala Leu Leu Lys Met Gln 
            20                  25                  30          


Cys Cys Gly Val Cys His Thr Asp Leu His Val Lys Asn Gly Asp Phe 
        35                  40                  45              


Gly Asp Lys Thr Gly Val Val Leu Gly His Glu Gly Ile Gly Val Val 
    50                  55                  60                  


Gln Glu Val Gly Pro Gly Val Thr Ser Leu Lys Pro Gly Asp Arg Ala 
65                  70                  75                  80  


Ser Val Ala Trp Phe Tyr Gln Gly Cys Gly His Cys Glu Tyr Cys Asn 
                85                  90                  95      


Ser Gly Asn Glu Thr Leu Cys Arg Asp Val Lys Asn Ser Gly Tyr Thr 
            100                 105                 110         


Val Asp Gly Gly Met Ala Glu Ala Cys Ile Val Lys Ala Asp Tyr Ser 
        115                 120                 125             


Val Lys Val Pro Asp Gly Leu Asp Ser Ala Ala Ala Ser Ser Ile Thr 
    130                 135                 140                 


Cys Ala Gly Val Thr Thr Tyr Lys Ala Val Lys Ile Ser Asn Val Arg 
145                 150                 155                 160 


Arg Ser Pro Gly Gln Trp Ile Ala Ile Tyr Gly Leu Gly Gly Leu Gly 
                165                 170                 175     


Asn Leu Ala Leu Gln Tyr Ala Lys Asn Val Phe Asn Ala Lys Val Ile 
            180                 185                 190         


Ala Ile Asp Val Asn Glu Glu Gln Leu Arg Phe Ala Ser Glu Met Gly 
        195                 200                 205             


Ala Asp Leu Val Val Asn Pro Leu Asn Glu Asp Ala Ala Lys Phe Ile 
    210                 215                 220                 


Gln Ala Lys Thr Gly Gly Ala His Ala Ala Val Val Thr Ala Val Ala 
225                 230                 235                 240 


Lys Gly Ala Phe Asn Ser Ala Val Asp Ala Leu Arg Ala Gly Gly Arg 
                245                 250                 255     


Leu Val Ala Val Gly Leu Pro Ser Glu Ser Met Asp Leu Asn Ile Pro 
            260                 265                 270         


Arg Leu Val Leu Asp Gly Ile Glu Val Val Gly Ser Leu Val Gly Thr 
        275                 280                 285             


Arg Gln Asp Leu Gln Glu Ala Phe Gln Phe Ala Ala Glu Gly Lys Val 
    290                 295                 300                 


Val Pro Lys Val Thr Leu Arg Pro Ile Glu His Ile Asn Gln Ile Phe 
305                 310                 315                 320 


Asp Glu Met Leu Glu Gly Lys Ile Lys Gly Arg Met Val Ile Gln Phe 
                325                 330                 335     


Glu Gly 
        


<210>  28
<211>  356
<212>  PRT
<213>  Pseudomonas putida

<400>  28

Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His Met Lys Ala Ala Val Val Ala Pro Gly Arg Arg Val 
            20                  25                  30          


Asp Val Val Glu Lys Thr Leu Arg Pro Leu Glu His Gly Glu Ala Leu 
        35                  40                  45              


Leu Lys Met Gln Cys Cys Gly Val Cys His Thr Asp Leu His Val Lys 
    50                  55                  60                  


Asn Gly Asp Phe Gly Asp Lys Thr Gly Val Val Leu Gly His Glu Gly 
65                  70                  75                  80  


Ile Gly Val Val Gln Glu Val Gly Pro Gly Val Thr Ser Leu Lys Pro 
                85                  90                  95      


Gly Asp Arg Ala Ser Val Ala Trp Phe Tyr Gln Gly Cys Gly His Cys 
            100                 105                 110         


Glu Tyr Cys Asn Ser Gly Asn Glu Thr Leu Cys Arg Asp Val Lys Asn 
        115                 120                 125             


Ser Gly Tyr Thr Val Asp Gly Gly Met Ala Glu Ala Cys Ile Val Lys 
    130                 135                 140                 


Ala Asp Tyr Ser Val Lys Val Pro Asp Gly Leu Asp Ser Ala Ala Ala 
145                 150                 155                 160 


Ser Ser Ile Thr Cys Ala Gly Val Thr Thr Tyr Lys Ala Val Lys Ile 
                165                 170                 175     


Ser Asn Val Arg Pro Gly Gln Trp Ile Ala Ile Tyr Gly Leu Gly Gly 
            180                 185                 190         


Leu Gly Asn Leu Ala Leu Gln Tyr Ala Lys Asn Val Phe Asn Ala Lys 
        195                 200                 205             


Val Ile Ala Ile Asp Val Asn Glu Glu Gln Leu Arg Phe Ala Ser Glu 
    210                 215                 220                 


Met Gly Ala Asp Leu Val Val Asn Pro Leu Asn Glu Asp Ala Ala Lys 
225                 230                 235                 240 


Phe Ile Gln Ala Lys Thr Gly Gly Ala His Ala Ala Val Val Thr Ala 
                245                 250                 255     


Val Ala Lys Gly Ala Phe Asn Ser Ala Val Asp Ala Leu Arg Ala Gly 
            260                 265                 270         


Gly Arg Leu Val Ala Val Gly Leu Pro Ser Glu Ser Met Asp Leu Asn 
        275                 280                 285             


Ile Pro Arg Leu Val Leu Asp Gly Ile Glu Val Val Gly Ser Leu Val 
    290                 295                 300                 


Gly Thr Arg Gln Asp Leu Gln Glu Ala Phe Gln Phe Ala Ala Glu Gly 
305                 310                 315                 320 


Lys Val Val Pro Lys Val Thr Leu Arg Pro Ile Glu His Ile Asn Gln 
                325                 330                 335     


Ile Phe Asp Glu Met Leu Glu Gly Lys Ile Lys Gly Arg Met Val Ile 
            340                 345                 350         


Gln Phe Glu Gly 
        355     


<210>  29
<211>  1164
<212>  DNA
<213>  Geobacillus thermodentrificans

<400>  29
atgcaaaatt ttacgtttcg caatccgacc aaactcattt ttgggagagg acaaattgag     60

cagctcaaag aagaagtgcc gaaatatggc aaaaaagtgc tgcttgtcta tgggggcggc    120

agcattaaac gaaacggact atatgatgaa gtcatgagcc tattgacgga cattggcgcc    180

gaagtcgttg aactgccagg cgtcgaaccg aacccgcgcc tttcgaccgt caaaaaaggg    240

gtagacattt gcagacggga aggaattgaa tttttgcttg ccgttggcgg cggcagtgtg    300

atcgactgta cgaaagcgat tgcagccggc gcgaagtttg atggcgatcc gtgggagttc    360

attacgaaaa aagcgactgt cactgaggcg ttgccgtttg ggacggtcct gacgctggca    420

gcgaccggct cggaaatgaa cgccggatcg gtgatcacca attgggagac gaaagaaaaa    480

tacggctggg gcagcccggt gacattcccg caattttcga ttttggatcc gacgtacacg    540

atgacggtgc cgaaagacca taccgtttac ggcatcgtcg atatgatgtc ccatgtgttt    600

gagcaatatt tccatcatac gccgaacacg ccgctgcaag accggatgtg cgaggcagtg    660

ttaaaaacgg tcattgaggc ggcgccaaaa ttggttgacg acttagagaa ctacgagctg    720

cgcgagacga tcatgtactc gggcacgatc gccttaaacg gctttttgca aatgggcgtg    780

cgcggtgatt gggcgacgca tgatatcgag catgcggtct ctgctgtata cgatatcccg    840

cacgccgggg gattggccat tttgttcccg aattggatga aacatgtgct tgatgaaaat    900

gtcagccgtt tcgcccaact ggcggtgcgc gtctttgacg tcgatccgac gggcaaaacg    960

gagcgcgacg tggcgcttga gggcatcgag cggttgcgcg cgttttggtc gagcctcggg   1020

gcgccgtctc gattggctga ttatggcatc ggcgaggaaa atctcgagct gatggcggat   1080

aaagcgatgg cgtttggtga gtttggccgt ttcaaaacgt taaatcgtga tgatgtgctc   1140

gccattttgc gtgcgtcttt ataa                                          1164


<210>  30
<211>  1170
<212>  DNA
<213>  Artificial

<220>
<223>  Gt_ADH codon optimized


<220>
<221>  CDS
<222>  (1)..(1170)

<400>  30
atg cag aac ttc acc ttt cgt aat ccg acg aaa ctg atc ttc ggt cgc       48
Met Gln Asn Phe Thr Phe Arg Asn Pro Thr Lys Leu Ile Phe Gly Arg         
1               5                   10                  15              

ggc caa atc gag caa ctg aag gaa gaa gtg cct aag tat ggt aag aaa       96
Gly Gln Ile Glu Gln Leu Lys Glu Glu Val Pro Lys Tyr Gly Lys Lys         
            20                  25                  30                  

gtt ctg ttg gtc tat ggt ggc ggc agc att aaa cgc aat ggc ctg tac      144
Val Leu Leu Val Tyr Gly Gly Gly Ser Ile Lys Arg Asn Gly Leu Tyr         
        35                  40                  45                      

gac gag gtg atg agc ctg ctg acg gat att ggt gca gaa gtc gtg gag      192
Asp Glu Val Met Ser Leu Leu Thr Asp Ile Gly Ala Glu Val Val Glu         
    50                  55                  60                          

ctg cca ggt gtt gag ccg aac ccg cgt ctg agc acc gtg aag aaa ggt      240
Leu Pro Gly Val Glu Pro Asn Pro Arg Leu Ser Thr Val Lys Lys Gly         
65                  70                  75                  80          

gtg gac atc tgt cgt cgt gag ggt atc gaa ttt ctg ctg gcg gtt ggt      288
Val Asp Ile Cys Arg Arg Glu Gly Ile Glu Phe Leu Leu Ala Val Gly         
                85                  90                  95              

ggc ggt agc gtc att gac tgc acg aag gca atc gcg gct ggt gcc aaa      336
Gly Gly Ser Val Ile Asp Cys Thr Lys Ala Ile Ala Ala Gly Ala Lys         
            100                 105                 110                 

ttt gat ggc gac ccg tgg gaa ttc atc acc aaa aag gcg acc gtt acg      384
Phe Asp Gly Asp Pro Trp Glu Phe Ile Thr Lys Lys Ala Thr Val Thr         
        115                 120                 125                     

gag gct ctg ccg ttt ggc acc gtc ttg acc ctg gcc gca acc ggt agc      432
Glu Ala Leu Pro Phe Gly Thr Val Leu Thr Leu Ala Ala Thr Gly Ser         
    130                 135                 140                         

gag atg aat gcg cga tcg ggc agc gtt att act aac tgg gag act aaa      480
Glu Met Asn Ala Arg Ser Gly Ser Val Ile Thr Asn Trp Glu Thr Lys         
145                 150                 155                 160         

gag aaa tac ggt tgg ggt tct ccg gtg acg ttc ccg cag ttc tcc att      528
Glu Lys Tyr Gly Trp Gly Ser Pro Val Thr Phe Pro Gln Phe Ser Ile         
                165                 170                 175             

ctg gac cca acc tat acg atg acg gtt cct aaa gac cac acc gtc tat      576
Leu Asp Pro Thr Tyr Thr Met Thr Val Pro Lys Asp His Thr Val Tyr         
            180                 185                 190                 

ggt atc gtg gat atg atg agc cac gtt ttt gaa caa tac ttc cac cac      624
Gly Ile Val Asp Met Met Ser His Val Phe Glu Gln Tyr Phe His His         
        195                 200                 205                     

acc ccg aac acc ccg ctg cag gat cgt atg tgc gaa gca gta ctg aaa      672
Thr Pro Asn Thr Pro Leu Gln Asp Arg Met Cys Glu Ala Val Leu Lys         
    210                 215                 220                         

acg gtt atc gaa gcg gca ccg aaa ctg gtc gat gat ctg gag aat tac      720
Thr Val Ile Glu Ala Ala Pro Lys Leu Val Asp Asp Leu Glu Asn Tyr         
225                 230                 235                 240         

gaa ctg cgt gaa acc att atg tat tcg ggc act atc gca ctg aac ggt      768
Glu Leu Arg Glu Thr Ile Met Tyr Ser Gly Thr Ile Ala Leu Asn Gly         
                245                 250                 255             

ttc ctg cag atg ggc gtt cgc ggt gat tgg gcg acc cat gac atc gag      816
Phe Leu Gln Met Gly Val Arg Gly Asp Trp Ala Thr His Asp Ile Glu         
            260                 265                 270                 

cat gca gtc tct gct gtg tac gat atc ccg cac gcc ggt ggc ctg gcc      864
His Ala Val Ser Ala Val Tyr Asp Ile Pro His Ala Gly Gly Leu Ala         
        275                 280                 285                     

att ttg ttc ccg aat tgg atg aag cat gtc ctg gat gag aac gtt agc      912
Ile Leu Phe Pro Asn Trp Met Lys His Val Leu Asp Glu Asn Val Ser         
    290                 295                 300                         

cgc ttt gcg caa ctg gcg gtg cgt gtg ttt gac gtc gac ccg acg ggt      960
Arg Phe Ala Gln Leu Ala Val Arg Val Phe Asp Val Asp Pro Thr Gly         
305                 310                 315                 320         

aag acc gag cgt gat gtg gcc ctg gaa ggt att gag cgt ctg cgt gcg     1008
Lys Thr Glu Arg Asp Val Ala Leu Glu Gly Ile Glu Arg Leu Arg Ala         
                325                 330                 335             

ttt tgg agc agc ctg ggc gct ccg tcc cgc ctg gca gat tac ggt att     1056
Phe Trp Ser Ser Leu Gly Ala Pro Ser Arg Leu Ala Asp Tyr Gly Ile         
            340                 345                 350                 

ggc gaa gaa aat ctg gag ctg atg gcc gac aag gcg atg gcg ttt ggt     1104
Gly Glu Glu Asn Leu Glu Leu Met Ala Asp Lys Ala Met Ala Phe Gly         
        355                 360                 365                     

gag ttc ggc cgt ttc aag acc ttg aac cgc gac gac gtt ttg gcg att     1152
Glu Phe Gly Arg Phe Lys Thr Leu Asn Arg Asp Asp Val Leu Ala Ile         
    370                 375                 380                         

ttg cgt gcg agc ctg taa                                             1170
Leu Arg Ala Ser Leu                                                     
385                                                                     


<210>  31
<211>  389
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic Construct

<400>  31

Met Gln Asn Phe Thr Phe Arg Asn Pro Thr Lys Leu Ile Phe Gly Arg 
1               5                   10                  15      


Gly Gln Ile Glu Gln Leu Lys Glu Glu Val Pro Lys Tyr Gly Lys Lys 
            20                  25                  30          


Val Leu Leu Val Tyr Gly Gly Gly Ser Ile Lys Arg Asn Gly Leu Tyr 
        35                  40                  45              


Asp Glu Val Met Ser Leu Leu Thr Asp Ile Gly Ala Glu Val Val Glu 
    50                  55                  60                  


Leu Pro Gly Val Glu Pro Asn Pro Arg Leu Ser Thr Val Lys Lys Gly 
65                  70                  75                  80  


Val Asp Ile Cys Arg Arg Glu Gly Ile Glu Phe Leu Leu Ala Val Gly 
                85                  90                  95      


Gly Gly Ser Val Ile Asp Cys Thr Lys Ala Ile Ala Ala Gly Ala Lys 
            100                 105                 110         


Phe Asp Gly Asp Pro Trp Glu Phe Ile Thr Lys Lys Ala Thr Val Thr 
        115                 120                 125             


Glu Ala Leu Pro Phe Gly Thr Val Leu Thr Leu Ala Ala Thr Gly Ser 
    130                 135                 140                 


Glu Met Asn Ala Arg Ser Gly Ser Val Ile Thr Asn Trp Glu Thr Lys 
145                 150                 155                 160 


Glu Lys Tyr Gly Trp Gly Ser Pro Val Thr Phe Pro Gln Phe Ser Ile 
                165                 170                 175     


Leu Asp Pro Thr Tyr Thr Met Thr Val Pro Lys Asp His Thr Val Tyr 
            180                 185                 190         


Gly Ile Val Asp Met Met Ser His Val Phe Glu Gln Tyr Phe His His 
        195                 200                 205             


Thr Pro Asn Thr Pro Leu Gln Asp Arg Met Cys Glu Ala Val Leu Lys 
    210                 215                 220                 


Thr Val Ile Glu Ala Ala Pro Lys Leu Val Asp Asp Leu Glu Asn Tyr 
225                 230                 235                 240 


Glu Leu Arg Glu Thr Ile Met Tyr Ser Gly Thr Ile Ala Leu Asn Gly 
                245                 250                 255     


Phe Leu Gln Met Gly Val Arg Gly Asp Trp Ala Thr His Asp Ile Glu 
            260                 265                 270         


His Ala Val Ser Ala Val Tyr Asp Ile Pro His Ala Gly Gly Leu Ala 
        275                 280                 285             


Ile Leu Phe Pro Asn Trp Met Lys His Val Leu Asp Glu Asn Val Ser 
    290                 295                 300                 


Arg Phe Ala Gln Leu Ala Val Arg Val Phe Asp Val Asp Pro Thr Gly 
305                 310                 315                 320 


Lys Thr Glu Arg Asp Val Ala Leu Glu Gly Ile Glu Arg Leu Arg Ala 
                325                 330                 335     


Phe Trp Ser Ser Leu Gly Ala Pro Ser Arg Leu Ala Asp Tyr Gly Ile 
            340                 345                 350         


Gly Glu Glu Asn Leu Glu Leu Met Ala Asp Lys Ala Met Ala Phe Gly 
        355                 360                 365             


Glu Phe Gly Arg Phe Lys Thr Leu Asn Arg Asp Asp Val Leu Ala Ile 
    370                 375                 380                 


Leu Arg Ala Ser Leu 
385                 


<210>  32
<211>  407
<212>  PRT
<213>  Geobacillus thermodentrificans

<400>  32

Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His Met Gln Asn Phe Thr Phe Arg Asn Pro Thr Lys Leu 
            20                  25                  30          


Ile Phe Gly Arg Gly Gln Ile Glu Gln Leu Lys Glu Glu Val Pro Lys 
        35                  40                  45              


Tyr Gly Lys Lys Val Leu Leu Val Tyr Gly Gly Gly Ser Ile Lys Arg 
    50                  55                  60                  


Asn Gly Leu Tyr Asp Glu Val Met Ser Leu Leu Thr Asp Ile Gly Ala 
65                  70                  75                  80  


Glu Val Val Glu Leu Pro Gly Val Glu Pro Asn Pro Arg Leu Ser Thr 
                85                  90                  95      


Val Lys Lys Gly Val Asp Ile Cys Arg Arg Glu Gly Ile Glu Phe Leu 
            100                 105                 110         


Leu Ala Val Gly Gly Gly Ser Val Ile Asp Cys Thr Lys Ala Ile Ala 
        115                 120                 125             


Ala Gly Ala Lys Phe Asp Gly Asp Pro Trp Glu Phe Ile Thr Lys Lys 
    130                 135                 140                 


Ala Thr Val Thr Glu Ala Leu Pro Phe Gly Thr Val Leu Thr Leu Ala 
145                 150                 155                 160 


Ala Thr Gly Ser Glu Met Asn Ala Gly Ser Val Ile Thr Asn Trp Glu 
                165                 170                 175     


Thr Lys Glu Lys Tyr Gly Trp Gly Ser Pro Val Thr Phe Pro Gln Phe 
            180                 185                 190         


Ser Ile Leu Asp Pro Thr Tyr Thr Met Thr Val Pro Lys Asp His Thr 
        195                 200                 205             


Val Tyr Gly Ile Val Asp Met Met Ser His Val Phe Glu Gln Tyr Phe 
    210                 215                 220                 


His His Thr Pro Asn Thr Pro Leu Gln Asp Arg Met Cys Glu Ala Val 
225                 230                 235                 240 


Leu Lys Thr Val Ile Glu Ala Ala Pro Lys Leu Val Asp Asp Leu Glu 
                245                 250                 255     


Asn Tyr Glu Leu Arg Glu Thr Ile Met Tyr Ser Gly Thr Ile Ala Leu 
            260                 265                 270         


Asn Gly Phe Leu Gln Met Gly Val Arg Gly Asp Trp Ala Thr His Asp 
        275                 280                 285             


Ile Glu His Ala Val Ser Ala Val Tyr Asp Ile Pro His Ala Gly Gly 
    290                 295                 300                 


Leu Ala Ile Leu Phe Pro Asn Trp Met Lys His Val Leu Asp Glu Asn 
305                 310                 315                 320 


Val Ser Arg Phe Ala Gln Leu Ala Val Arg Val Phe Asp Val Asp Pro 
                325                 330                 335     


Thr Gly Lys Thr Glu Arg Asp Val Ala Leu Glu Gly Ile Glu Arg Leu 
            340                 345                 350         


Arg Ala Phe Trp Ser Ser Leu Gly Ala Pro Ser Arg Leu Ala Asp Tyr 
        355                 360                 365             


Gly Ile Gly Glu Glu Asn Leu Glu Leu Met Ala Asp Lys Ala Met Ala 
    370                 375                 380                 


Phe Gly Glu Phe Gly Arg Phe Lys Thr Leu Asn Arg Asp Asp Val Leu 
385                 390                 395                 400 


Ala Ile Leu Arg Ala Ser Leu 
                405         


<210>  33
<211>  969
<212>  DNA
<213>  Archaeoglobus fulgidus


<220>
<221>  CDS
<222>  (1)..(969)

<400>  33
atg gag act ctt att ttg act cag gag gaa gta gaa tcc ctg ata tcg       48
Met Glu Thr Leu Ile Leu Thr Gln Glu Glu Val Glu Ser Leu Ile Ser         
1               5                   10                  15              

atg gac gag gcg atg aat gct gtt gag gag gcg ttc aga ctt tac gct       96
Met Asp Glu Ala Met Asn Ala Val Glu Glu Ala Phe Arg Leu Tyr Ala         
            20                  25                  30                  

ctt ggt aaa gct caa atg ccg cca aag gtg tat ctt gag ttc gaa aaa      144
Leu Gly Lys Ala Gln Met Pro Pro Lys Val Tyr Leu Glu Phe Glu Lys         
        35                  40                  45                      

ggc gat tta agg gct atg ccg gcg cat tta atg ggg tac gca gga ttg      192
Gly Asp Leu Arg Ala Met Pro Ala His Leu Met Gly Tyr Ala Gly Leu         
    50                  55                  60                          

aag tgg gtg aac tcc cat ccc gga aat ccg gat aaa ggc ctg cca aca      240
Lys Trp Val Asn Ser His Pro Gly Asn Pro Asp Lys Gly Leu Pro Thr         
65                  70                  75                  80          

gtc atg gcg ttg atg ata ctg aac agc cct gag acg ggg ttt ccc ttg      288
Val Met Ala Leu Met Ile Leu Asn Ser Pro Glu Thr Gly Phe Pro Leu         
                85                  90                  95              

gct gtg atg gac gca acc tac acg acc tct ctg aga acc ggt gct gct      336
Ala Val Met Asp Ala Thr Tyr Thr Thr Ser Leu Arg Thr Gly Ala Ala         
            100                 105                 110                 

gga gga ata gcg gcg aag tat ctg gca agg aag aac agc agc gtt ttt      384
Gly Gly Ile Ala Ala Lys Tyr Leu Ala Arg Lys Asn Ser Ser Val Phe         
        115                 120                 125                     

gga ttt atc ggc tgc gga act cag gcg tat ttc cag ctc gaa gct ctg      432
Gly Phe Ile Gly Cys Gly Thr Gln Ala Tyr Phe Gln Leu Glu Ala Leu         
    130                 135                 140                         

agg aga gtg ttt gat ata gga gaa gtc aag gcc tac gat gtt agg gaa      480
Arg Arg Val Phe Asp Ile Gly Glu Val Lys Ala Tyr Asp Val Arg Glu         
145                 150                 155                 160         

aag gca gcg aaa aag ttc gtt agc tat tgc gaa gat agg gga atc tca      528
Lys Ala Ala Lys Lys Phe Val Ser Tyr Cys Glu Asp Arg Gly Ile Ser         
                165                 170                 175             

gct tcc gtg cag cct gct gag gag gcg agc aga tgc gat gtc ctt gtc      576
Ala Ser Val Gln Pro Ala Glu Glu Ala Ser Arg Cys Asp Val Leu Val         
            180                 185                 190                 

acc aca act ccg tca cga aaa cca gtt gtt aag gct gag tgg gtt gag      624
Thr Thr Thr Pro Ser Arg Lys Pro Val Val Lys Ala Glu Trp Val Glu         
        195                 200                 205                     

gag ggg acg cac ata aac gca atc ggt gcg gac ggg cct gga aag cag      672
Glu Gly Thr His Ile Asn Ala Ile Gly Ala Asp Gly Pro Gly Lys Gln         
    210                 215                 220                         

gaa ctg gat gta gaa ata ctt aaa aag gct aaa att gtt gtc gat gac      720
Glu Leu Asp Val Glu Ile Leu Lys Lys Ala Lys Ile Val Val Asp Asp         
225                 230                 235                 240         

ctt gaa cag gcc aag cac ggc ggg gag att aat gtg gcg gtt tca aaa      768
Leu Glu Gln Ala Lys His Gly Gly Glu Ile Asn Val Ala Val Ser Lys         
                245                 250                 255             

ggc gtc ata ggt gtt gaa gat gtt cac gca acc atc ggg gag gtt ata      816
Gly Val Ile Gly Val Glu Asp Val His Ala Thr Ile Gly Glu Val Ile         
            260                 265                 270                 

gcc ggt ctg aag gat gga agg gag agc gat gag gag att aca atc ttc      864
Ala Gly Leu Lys Asp Gly Arg Glu Ser Asp Glu Glu Ile Thr Ile Phe         
        275                 280                 285                     

gac tcc aca ggc ttg gcg att cag gac gtg gct gtt gca aaa gtc gtt      912
Asp Ser Thr Gly Leu Ala Ile Gln Asp Val Ala Val Ala Lys Val Val         
    290                 295                 300                         

tat gag aac gcc ctt agc aag aac gtg ggg agt aaa ata aag ttt ttc      960
Tyr Glu Asn Ala Leu Ser Lys Asn Val Gly Ser Lys Ile Lys Phe Phe         
305                 310                 315                 320         

agg ata tga                                                          969
Arg Ile                                                                 
                                                                        


<210>  34
<211>  322
<212>  PRT
<213>  Archaeoglobus fulgidus

<400>  34

Met Glu Thr Leu Ile Leu Thr Gln Glu Glu Val Glu Ser Leu Ile Ser 
1               5                   10                  15      


Met Asp Glu Ala Met Asn Ala Val Glu Glu Ala Phe Arg Leu Tyr Ala 
            20                  25                  30          


Leu Gly Lys Ala Gln Met Pro Pro Lys Val Tyr Leu Glu Phe Glu Lys 
        35                  40                  45              


Gly Asp Leu Arg Ala Met Pro Ala His Leu Met Gly Tyr Ala Gly Leu 
    50                  55                  60                  


Lys Trp Val Asn Ser His Pro Gly Asn Pro Asp Lys Gly Leu Pro Thr 
65                  70                  75                  80  


Val Met Ala Leu Met Ile Leu Asn Ser Pro Glu Thr Gly Phe Pro Leu 
                85                  90                  95      


Ala Val Met Asp Ala Thr Tyr Thr Thr Ser Leu Arg Thr Gly Ala Ala 
            100                 105                 110         


Gly Gly Ile Ala Ala Lys Tyr Leu Ala Arg Lys Asn Ser Ser Val Phe 
        115                 120                 125             


Gly Phe Ile Gly Cys Gly Thr Gln Ala Tyr Phe Gln Leu Glu Ala Leu 
    130                 135                 140                 


Arg Arg Val Phe Asp Ile Gly Glu Val Lys Ala Tyr Asp Val Arg Glu 
145                 150                 155                 160 


Lys Ala Ala Lys Lys Phe Val Ser Tyr Cys Glu Asp Arg Gly Ile Ser 
                165                 170                 175     


Ala Ser Val Gln Pro Ala Glu Glu Ala Ser Arg Cys Asp Val Leu Val 
            180                 185                 190         


Thr Thr Thr Pro Ser Arg Lys Pro Val Val Lys Ala Glu Trp Val Glu 
        195                 200                 205             


Glu Gly Thr His Ile Asn Ala Ile Gly Ala Asp Gly Pro Gly Lys Gln 
    210                 215                 220                 


Glu Leu Asp Val Glu Ile Leu Lys Lys Ala Lys Ile Val Val Asp Asp 
225                 230                 235                 240 


Leu Glu Gln Ala Lys His Gly Gly Glu Ile Asn Val Ala Val Ser Lys 
                245                 250                 255     


Gly Val Ile Gly Val Glu Asp Val His Ala Thr Ile Gly Glu Val Ile 
            260                 265                 270         


Ala Gly Leu Lys Asp Gly Arg Glu Ser Asp Glu Glu Ile Thr Ile Phe 
        275                 280                 285             


Asp Ser Thr Gly Leu Ala Ile Gln Asp Val Ala Val Ala Lys Val Val 
    290                 295                 300                 


Tyr Glu Asn Ala Leu Ser Lys Asn Val Gly Ser Lys Ile Lys Phe Phe 
305                 310                 315                 320 


Arg Ile 
        


<210>  35
<211>  342
<212>  PRT
<213>  Archaeoglobus fulgidus

<400>  35

Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His Met Glu Thr Leu Ile Leu Thr Gln Glu Glu Val Glu 
            20                  25                  30          


Ser Leu Ile Ser Met Asp Glu Ala Met Asn Ala Val Glu Glu Ala Phe 
        35                  40                  45              


Arg Leu Tyr Ala Leu Gly Lys Ala Gln Met Pro Pro Lys Val Tyr Leu 
    50                  55                  60                  


Glu Phe Glu Lys Gly Asp Leu Arg Ala Met Pro Ala His Leu Met Gly 
65                  70                  75                  80  


Tyr Ala Gly Leu Lys Trp Val Asn Ser His Pro Gly Asn Pro Asp Lys 
                85                  90                  95      


Gly Leu Pro Thr Val Met Ala Leu Met Ile Leu Asn Ser Pro Glu Thr 
            100                 105                 110         


Gly Phe Pro Leu Ala Val Met Asp Ala Thr Tyr Thr Thr Ser Leu Arg 
        115                 120                 125             


Thr Gly Ala Ala Gly Gly Ile Ala Ala Lys Tyr Leu Ala Arg Lys Asn 
    130                 135                 140                 


Ser Ser Val Phe Gly Phe Ile Gly Cys Gly Thr Gln Ala Tyr Phe Gln 
145                 150                 155                 160 


Leu Glu Ala Leu Arg Arg Val Phe Asp Ile Gly Glu Val Lys Ala Tyr 
                165                 170                 175     


Asp Val Arg Glu Lys Ala Ala Lys Lys Phe Val Ser Tyr Cys Glu Asp 
            180                 185                 190         


Arg Gly Ile Ser Ala Ser Val Gln Pro Ala Glu Glu Ala Ser Arg Cys 
        195                 200                 205             


Asp Val Leu Val Thr Thr Thr Pro Ser Arg Lys Pro Val Val Lys Ala 
    210                 215                 220                 


Glu Trp Val Glu Glu Gly Thr His Ile Asn Ala Ile Gly Ala Asp Gly 
225                 230                 235                 240 


Pro Gly Lys Gln Glu Leu Asp Val Glu Ile Leu Lys Lys Ala Lys Ile 
                245                 250                 255     


Val Val Asp Asp Leu Glu Gln Ala Lys His Gly Gly Glu Ile Asn Val 
            260                 265                 270         


Ala Val Ser Lys Gly Val Ile Gly Val Glu Asp Val His Ala Thr Ile 
        275                 280                 285             


Gly Glu Val Ile Ala Gly Leu Lys Asp Gly Arg Glu Ser Asp Glu Glu 
    290                 295                 300                 


Ile Thr Ile Phe Asp Ser Thr Gly Leu Ala Ile Gln Asp Val Ala Val 
305                 310                 315                 320 


Ala Lys Val Val Tyr Glu Asn Ala Leu Ser Lys Asn Val Gly Ser Lys 
                325                 330                 335     


Ile Lys Phe Phe Arg Ile 
            340         


<210>  36
<211>  36
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Forward Primer

<400>  36
cgcgcggcag ccatatggag actcttattt tgactc                               36


<210>  37
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Rev Primer

<400>  37
gtgcggccgc aagcttttat atcctgaaaa actttatttt ac                        42


<210>  38
<211>  1110
<212>  DNA
<213>  Thermus thermophilus


<220>
<221>  CDS
<222>  (1)..(1110)

<400>  38
atg gtg atc ggc gtg ccg aag gag atc aag acc ttg gag aac cgg gtg       48
Met Val Ile Gly Val Pro Lys Glu Ile Lys Thr Leu Glu Asn Arg Val         
1               5                   10                  15              

gcc ctc acg ccc ggc ggg gtg gag agc ctg gtc agg cgc ggc cac acc       96
Ala Leu Thr Pro Gly Gly Val Glu Ser Leu Val Arg Arg Gly His Thr         
            20                  25                  30                  

gtg ctg gtg gag cgg ggg gcc ggg gag ggc tcg ggg ctt tcc gac gcg      144
Val Leu Val Glu Arg Gly Ala Gly Glu Gly Ser Gly Leu Ser Asp Ala         
        35                  40                  45                      

gag tac gcc cgg gcc ggg gcc gag ctc gtg ggc cgg gag gag gcc tgg      192
Glu Tyr Ala Arg Ala Gly Ala Glu Leu Val Gly Arg Glu Glu Ala Trp         
    50                  55                  60                          

ggt gcg gag atg gtg gtg aag gtg aag gag ccc cta ccc gag gag tac      240
Gly Ala Glu Met Val Val Lys Val Lys Glu Pro Leu Pro Glu Glu Tyr         
65                  70                  75                  80          

ggc ttc ctg cgg gag ggc ctc atc ctc ttc acc tac ctt cac ctg gcc      288
Gly Phe Leu Arg Glu Gly Leu Ile Leu Phe Thr Tyr Leu His Leu Ala         
                85                  90                  95              

gcg gac cgc ggc ctc acc gag gcc atg ctc cgt agc ggg gtc acg ggc      336
Ala Asp Arg Gly Leu Thr Glu Ala Met Leu Arg Ser Gly Val Thr Gly         
            100                 105                 110                 

atc gcc tac gag acc gtc cag ctt ccc gac ggc acc ctc ccc ctc ctc      384
Ile Ala Tyr Glu Thr Val Gln Leu Pro Asp Gly Thr Leu Pro Leu Leu         
        115                 120                 125                     

gtc ccc atg agc gag gtg gcg ggg cgg atg gcc ccc cag gtg ggg gcc      432
Val Pro Met Ser Glu Val Ala Gly Arg Met Ala Pro Gln Val Gly Ala         
    130                 135                 140                         

cag ttc ctg gag aag ccc aag ggg ggc cgg ggg gtc ctc ctc ggg ggg      480
Gln Phe Leu Glu Lys Pro Lys Gly Gly Arg Gly Val Leu Leu Gly Gly         
145                 150                 155                 160         

gtg ccg ggg gtg gcc ccg gcc agc gtg gtg atc ctc ggg ggc ggg acc      528
Val Pro Gly Val Ala Pro Ala Ser Val Val Ile Leu Gly Gly Gly Thr         
                165                 170                 175             

gtg ggc acc aac gcg gcc aag atc gcc ctg ggg atg ggg gcc cag gtg      576
Val Gly Thr Asn Ala Ala Lys Ile Ala Leu Gly Met Gly Ala Gln Val         
            180                 185                 190                 

acc atc ctg gac gtg aac cac aag cgc ctc cag tat ctg gac gac gtc      624
Thr Ile Leu Asp Val Asn His Lys Arg Leu Gln Tyr Leu Asp Asp Val         
        195                 200                 205                     

ttc ggc ggg cgg gtg atc acc ctc acc gcc acc gag gcc aac atc aaa      672
Phe Gly Gly Arg Val Ile Thr Leu Thr Ala Thr Glu Ala Asn Ile Lys         
    210                 215                 220                         

aag agc gtc cag cac gcg gac ctc ctc atc ggg gcc gtc ctc gtc ccc      720
Lys Ser Val Gln His Ala Asp Leu Leu Ile Gly Ala Val Leu Val Pro         
225                 230                 235                 240         

ggg gcc aag gcc ccc aag ctc gtc acc cgg gac atg ctc tct ctg atg      768
Gly Ala Lys Ala Pro Lys Leu Val Thr Arg Asp Met Leu Ser Leu Met         
                245                 250                 255             

aag gag gga gcg gtg atc gtg gac gtg gcc gtg gac cag ggg ggg tgc      816
Lys Glu Gly Ala Val Ile Val Asp Val Ala Val Asp Gln Gly Gly Cys         
            260                 265                 270                 

gtg gag acc atc cgg ccc acc acc cac gcc gag ccc acc tac gtg gtg      864
Val Glu Thr Ile Arg Pro Thr Thr His Ala Glu Pro Thr Tyr Val Val         
        275                 280                 285                     

gac ggg gtg gtc cac tac ggg gtg gcc aac atg ccc ggg gcg gtg ccc      912
Asp Gly Val Val His Tyr Gly Val Ala Asn Met Pro Gly Ala Val Pro         
    290                 295                 300                         

agg acc agc acc ttc gcc ctc acc aac cag acc ctg ccc tac gtg ttg      960
Arg Thr Ser Thr Phe Ala Leu Thr Asn Gln Thr Leu Pro Tyr Val Leu         
305                 310                 315                 320         

aag ctc gcg gag aag ggg ctg gac gcc ctt ctg gag gac gcg gcc ctt     1008
Lys Leu Ala Glu Lys Gly Leu Asp Ala Leu Leu Glu Asp Ala Ala Leu         
                325                 330                 335             

ctc aag ggg ctc aac acc cac aaa ggc cgc ctc acc cac ccc ggg gtg     1056
Leu Lys Gly Leu Asn Thr His Lys Gly Arg Leu Thr His Pro Gly Val         
            340                 345                 350                 

gcc gag gcc ttc ggc ctg ccc tac acg cct ccc gag gag gcc ttg agg     1104
Ala Glu Ala Phe Gly Leu Pro Tyr Thr Pro Pro Glu Glu Ala Leu Arg         
        355                 360                 365                     

ggg tga                                                             1110
Gly                                                                     
                                                                        


<210>  39
<211>  369
<212>  PRT
<213>  Thermus thermophilus

<400>  39

Met Val Ile Gly Val Pro Lys Glu Ile Lys Thr Leu Glu Asn Arg Val 
1               5                   10                  15      


Ala Leu Thr Pro Gly Gly Val Glu Ser Leu Val Arg Arg Gly His Thr 
            20                  25                  30          


Val Leu Val Glu Arg Gly Ala Gly Glu Gly Ser Gly Leu Ser Asp Ala 
        35                  40                  45              


Glu Tyr Ala Arg Ala Gly Ala Glu Leu Val Gly Arg Glu Glu Ala Trp 
    50                  55                  60                  


Gly Ala Glu Met Val Val Lys Val Lys Glu Pro Leu Pro Glu Glu Tyr 
65                  70                  75                  80  


Gly Phe Leu Arg Glu Gly Leu Ile Leu Phe Thr Tyr Leu His Leu Ala 
                85                  90                  95      


Ala Asp Arg Gly Leu Thr Glu Ala Met Leu Arg Ser Gly Val Thr Gly 
            100                 105                 110         


Ile Ala Tyr Glu Thr Val Gln Leu Pro Asp Gly Thr Leu Pro Leu Leu 
        115                 120                 125             


Val Pro Met Ser Glu Val Ala Gly Arg Met Ala Pro Gln Val Gly Ala 
    130                 135                 140                 


Gln Phe Leu Glu Lys Pro Lys Gly Gly Arg Gly Val Leu Leu Gly Gly 
145                 150                 155                 160 


Val Pro Gly Val Ala Pro Ala Ser Val Val Ile Leu Gly Gly Gly Thr 
                165                 170                 175     


Val Gly Thr Asn Ala Ala Lys Ile Ala Leu Gly Met Gly Ala Gln Val 
            180                 185                 190         


Thr Ile Leu Asp Val Asn His Lys Arg Leu Gln Tyr Leu Asp Asp Val 
        195                 200                 205             


Phe Gly Gly Arg Val Ile Thr Leu Thr Ala Thr Glu Ala Asn Ile Lys 
    210                 215                 220                 


Lys Ser Val Gln His Ala Asp Leu Leu Ile Gly Ala Val Leu Val Pro 
225                 230                 235                 240 


Gly Ala Lys Ala Pro Lys Leu Val Thr Arg Asp Met Leu Ser Leu Met 
                245                 250                 255     


Lys Glu Gly Ala Val Ile Val Asp Val Ala Val Asp Gln Gly Gly Cys 
            260                 265                 270         


Val Glu Thr Ile Arg Pro Thr Thr His Ala Glu Pro Thr Tyr Val Val 
        275                 280                 285             


Asp Gly Val Val His Tyr Gly Val Ala Asn Met Pro Gly Ala Val Pro 
    290                 295                 300                 


Arg Thr Ser Thr Phe Ala Leu Thr Asn Gln Thr Leu Pro Tyr Val Leu 
305                 310                 315                 320 


Lys Leu Ala Glu Lys Gly Leu Asp Ala Leu Leu Glu Asp Ala Ala Leu 
                325                 330                 335     


Leu Lys Gly Leu Asn Thr His Lys Gly Arg Leu Thr His Pro Gly Val 
            340                 345                 350         


Ala Glu Ala Phe Gly Leu Pro Tyr Thr Pro Pro Glu Glu Ala Leu Arg 
        355                 360                 365             


Gly 
    


<210>  40
<211>  389
<212>  PRT
<213>  Thermus thermophilus

<400>  40

Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His Met Val Ile Gly Val Pro Lys Glu Ile Lys Thr Leu 
            20                  25                  30          


Glu Asn Arg Val Ala Leu Thr Pro Gly Gly Val Glu Ser Leu Val Arg 
        35                  40                  45              


Arg Gly His Thr Val Leu Val Glu Arg Gly Ala Gly Glu Gly Ser Gly 
    50                  55                  60                  


Leu Ser Asp Ala Glu Tyr Ala Arg Ala Gly Ala Glu Leu Val Gly Arg 
65                  70                  75                  80  


Glu Glu Ala Trp Gly Ala Glu Met Val Val Lys Val Lys Glu Pro Leu 
                85                  90                  95      


Pro Glu Glu Tyr Gly Phe Leu Arg Glu Gly Leu Ile Leu Phe Thr Tyr 
            100                 105                 110         


Leu His Leu Ala Ala Asp Arg Gly Leu Thr Glu Ala Met Leu Arg Ser 
        115                 120                 125             


Gly Val Thr Gly Ile Ala Tyr Glu Thr Val Gln Leu Pro Asp Gly Thr 
    130                 135                 140                 


Leu Pro Leu Leu Val Pro Met Ser Glu Val Ala Gly Arg Met Ala Pro 
145                 150                 155                 160 


Gln Val Gly Ala Gln Phe Leu Glu Lys Pro Lys Gly Gly Arg Gly Val 
                165                 170                 175     


Leu Leu Gly Gly Val Pro Gly Val Ala Pro Ala Ser Val Val Ile Leu 
            180                 185                 190         


Gly Gly Gly Thr Val Gly Thr Asn Ala Ala Lys Ile Ala Leu Gly Met 
        195                 200                 205             


Gly Ala Gln Val Thr Ile Leu Asp Val Asn His Lys Arg Leu Gln Tyr 
    210                 215                 220                 


Leu Asp Asp Val Phe Gly Gly Arg Val Ile Thr Leu Thr Ala Thr Glu 
225                 230                 235                 240 


Ala Asn Ile Lys Lys Ser Val Gln His Ala Asp Leu Leu Ile Gly Ala 
                245                 250                 255     


Val Leu Val Pro Gly Ala Lys Ala Pro Lys Leu Val Thr Arg Asp Met 
            260                 265                 270         


Leu Ser Leu Met Lys Glu Gly Ala Val Ile Val Asp Val Ala Val Asp 
        275                 280                 285             


Gln Gly Gly Cys Val Glu Thr Ile Arg Pro Thr Thr His Ala Glu Pro 
    290                 295                 300                 


Thr Tyr Val Val Asp Gly Val Val His Tyr Gly Val Ala Asn Met Pro 
305                 310                 315                 320 


Gly Ala Val Pro Arg Thr Ser Thr Phe Ala Leu Thr Asn Gln Thr Leu 
                325                 330                 335     


Pro Tyr Val Leu Lys Leu Ala Glu Lys Gly Leu Asp Ala Leu Leu Glu 
            340                 345                 350         


Asp Ala Ala Leu Leu Lys Gly Leu Asn Thr His Lys Gly Arg Leu Thr 
        355                 360                 365             


His Pro Gly Val Ala Glu Ala Phe Gly Leu Pro Tyr Thr Pro Pro Glu 
    370                 375                 380                 


Glu Ala Leu Arg Gly 
385                 


<210>  41
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Forward Primer

<400>  41
cgcgcggcag ccatatggtg atcggcgtgc                                      30


<210>  42
<211>  35
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Reverse Primer

<400>  42
gtgcggccgc aagcttttac cccctcaagg cctcc                                35


<210>  43
<211>  1125
<212>  DNA
<213>  Vibrio proteolyticus


<220>
<221>  CDS
<222>  (1)..(1125)

<400>  43
atg atc att ggc gta cct aag gaa atc aaa aac cac gaa tac cgc gtc       48
Met Ile Ile Gly Val Pro Lys Glu Ile Lys Asn His Glu Tyr Arg Val         
1               5                   10                  15              

ggt atg att cca gca agt gtc cgt gag cta atc tca cat ggt cac caa       96
Gly Met Ile Pro Ala Ser Val Arg Glu Leu Ile Ser His Gly His Gln         
            20                  25                  30                  

gtt ttt gtt gaa acc aat gcc ggc gca ggc atc ggt ttt tca gac gat      144
Val Phe Val Glu Thr Asn Ala Gly Ala Gly Ile Gly Phe Ser Asp Asp         
        35                  40                  45                      

gat tac atc gct gta ggc gca tcc att ctt cct act gct gct gag gtt      192
Asp Tyr Ile Ala Val Gly Ala Ser Ile Leu Pro Thr Ala Ala Glu Val         
    50                  55                  60                          

ttc gct caa gca gat atg att gtt aag gtc aaa gaa cct caa gct gtc      240
Phe Ala Gln Ala Asp Met Ile Val Lys Val Lys Glu Pro Gln Ala Val         
65                  70                  75                  80          

gaa cga gct atg ctc aaa gaa ggg caa att tta ttt act tat ttg cac      288
Glu Arg Ala Met Leu Lys Glu Gly Gln Ile Leu Phe Thr Tyr Leu His         
                85                  90                  95              

ctg gca cca gat ttt cca caa act gaa gac ctg atc aag agc aaa gct      336
Leu Ala Pro Asp Phe Pro Gln Thr Glu Asp Leu Ile Lys Ser Lys Ala         
            100                 105                 110                 

gtc tgc ata gcc tat gag act gta aca gat aat atg ggt cgc ttg cca      384
Val Cys Ile Ala Tyr Glu Thr Val Thr Asp Asn Met Gly Arg Leu Pro         
        115                 120                 125                     

cta tta gca cca atg tct gaa gtt gcg ggt cgt atg tct atc cag gca      432
Leu Leu Ala Pro Met Ser Glu Val Ala Gly Arg Met Ser Ile Gln Ala         
    130                 135                 140                         

ggc gca caa act ctc gag aaa tct cac ggc ggc cgt ggc ctg ctg ctg      480
Gly Ala Gln Thr Leu Glu Lys Ser His Gly Gly Arg Gly Leu Leu Leu         
145                 150                 155                 160         

ggt ggc gta cca ggt gtt gaa cca gcg aaa gtt gtg atc gtc ggc ggc      528
Gly Gly Val Pro Gly Val Glu Pro Ala Lys Val Val Ile Val Gly Gly         
                165                 170                 175             

ggc gtc gtg ggt gct aac gca gct cgt atg gca gtt ggc atg cgc gcc      576
Gly Val Val Gly Ala Asn Ala Ala Arg Met Ala Val Gly Met Arg Ala         
            180                 185                 190                 

gac gtt act atc tta gac cgt aac atc gat aca ctg cgt aag ctg gat      624
Asp Val Thr Ile Leu Asp Arg Asn Ile Asp Thr Leu Arg Lys Leu Asp         
        195                 200                 205                     

gaa gag ttt cag ggc cgt gcc aaa gtg gtt tac tca act gaa gat gcg      672
Glu Glu Phe Gln Gly Arg Ala Lys Val Val Tyr Ser Thr Glu Asp Ala         
    210                 215                 220                         

att gaa aaa cac gtt ctg gcg gcc gat ctg gtt atc ggt gca gtt ctg      720
Ile Glu Lys His Val Leu Ala Ala Asp Leu Val Ile Gly Ala Val Leu         
225                 230                 235                 240         

atc cca ggt gcc gca gct cct aaa ctg gtg aca aaa gag cac atc gct      768
Ile Pro Gly Ala Ala Ala Pro Lys Leu Val Thr Lys Glu His Ile Ala         
                245                 250                 255             

aaa atg aaa cca ggc gcg gct gtt gtc gac gtt gct atc gac cag ggc      816
Lys Met Lys Pro Gly Ala Ala Val Val Asp Val Ala Ile Asp Gln Gly         
            260                 265                 270                 

ggt tgt ttt gaa act tct cac gcg aca acg cac gca gat cca act tac      864
Gly Cys Phe Glu Thr Ser His Ala Thr Thr His Ala Asp Pro Thr Tyr         
        275                 280                 285                     

atc gtt gat gat gtg gtt cac tac tgt gta gca aac atg cca ggg gct      912
Ile Val Asp Asp Val Val His Tyr Cys Val Ala Asn Met Pro Gly Ala         
    290                 295                 300                         

gtt gcc cgc act tcc act ttt gca ctg aac aac gca acc ctg cca tat      960
Val Ala Arg Thr Ser Thr Phe Ala Leu Asn Asn Ala Thr Leu Pro Tyr         
305                 310                 315                 320         

atc gtt aaa ctg gca aac aaa gga tac cgt gaa gct ctg ctg gct gac     1008
Ile Val Lys Leu Ala Asn Lys Gly Tyr Arg Glu Ala Leu Leu Ala Asp         
                325                 330                 335             

cac ggc ttc cta gaa ggc ctg aat gtt att cac ggt aaa gtg acg tgc     1056
His Gly Phe Leu Glu Gly Leu Asn Val Ile His Gly Lys Val Thr Cys         
            340                 345                 350                 

aaa gaa gtc gca gaa gcg ttt aat ctg gaa tac gta cag ccg gaa acc     1104
Lys Glu Val Ala Glu Ala Phe Asn Leu Glu Tyr Val Gln Pro Glu Thr         
        355                 360                 365                     

gcg atc gcc atg ttc aac taa                                         1125
Ala Ile Ala Met Phe Asn                                                 
    370                                                                 


<210>  44
<211>  374
<212>  PRT
<213>  Vibrio proteolyticus

<400>  44

Met Ile Ile Gly Val Pro Lys Glu Ile Lys Asn His Glu Tyr Arg Val 
1               5                   10                  15      


Gly Met Ile Pro Ala Ser Val Arg Glu Leu Ile Ser His Gly His Gln 
            20                  25                  30          


Val Phe Val Glu Thr Asn Ala Gly Ala Gly Ile Gly Phe Ser Asp Asp 
        35                  40                  45              


Asp Tyr Ile Ala Val Gly Ala Ser Ile Leu Pro Thr Ala Ala Glu Val 
    50                  55                  60                  


Phe Ala Gln Ala Asp Met Ile Val Lys Val Lys Glu Pro Gln Ala Val 
65                  70                  75                  80  


Glu Arg Ala Met Leu Lys Glu Gly Gln Ile Leu Phe Thr Tyr Leu His 
                85                  90                  95      


Leu Ala Pro Asp Phe Pro Gln Thr Glu Asp Leu Ile Lys Ser Lys Ala 
            100                 105                 110         


Val Cys Ile Ala Tyr Glu Thr Val Thr Asp Asn Met Gly Arg Leu Pro 
        115                 120                 125             


Leu Leu Ala Pro Met Ser Glu Val Ala Gly Arg Met Ser Ile Gln Ala 
    130                 135                 140                 


Gly Ala Gln Thr Leu Glu Lys Ser His Gly Gly Arg Gly Leu Leu Leu 
145                 150                 155                 160 


Gly Gly Val Pro Gly Val Glu Pro Ala Lys Val Val Ile Val Gly Gly 
                165                 170                 175     


Gly Val Val Gly Ala Asn Ala Ala Arg Met Ala Val Gly Met Arg Ala 
            180                 185                 190         


Asp Val Thr Ile Leu Asp Arg Asn Ile Asp Thr Leu Arg Lys Leu Asp 
        195                 200                 205             


Glu Glu Phe Gln Gly Arg Ala Lys Val Val Tyr Ser Thr Glu Asp Ala 
    210                 215                 220                 


Ile Glu Lys His Val Leu Ala Ala Asp Leu Val Ile Gly Ala Val Leu 
225                 230                 235                 240 


Ile Pro Gly Ala Ala Ala Pro Lys Leu Val Thr Lys Glu His Ile Ala 
                245                 250                 255     


Lys Met Lys Pro Gly Ala Ala Val Val Asp Val Ala Ile Asp Gln Gly 
            260                 265                 270         


Gly Cys Phe Glu Thr Ser His Ala Thr Thr His Ala Asp Pro Thr Tyr 
        275                 280                 285             


Ile Val Asp Asp Val Val His Tyr Cys Val Ala Asn Met Pro Gly Ala 
    290                 295                 300                 


Val Ala Arg Thr Ser Thr Phe Ala Leu Asn Asn Ala Thr Leu Pro Tyr 
305                 310                 315                 320 


Ile Val Lys Leu Ala Asn Lys Gly Tyr Arg Glu Ala Leu Leu Ala Asp 
                325                 330                 335     


His Gly Phe Leu Glu Gly Leu Asn Val Ile His Gly Lys Val Thr Cys 
            340                 345                 350         


Lys Glu Val Ala Glu Ala Phe Asn Leu Glu Tyr Val Gln Pro Glu Thr 
        355                 360                 365             


Ala Ile Ala Met Phe Asn 
    370                 


<210>  45
<211>  394
<212>  PRT
<213>  Vibrio proteolyticus

<400>  45

Met Gly Ser Ser His His His His His His Ser Ser Gly Leu Val Pro 
1               5                   10                  15      


Arg Gly Ser His Met Ile Ile Gly Val Pro Lys Glu Ile Lys Asn His 
            20                  25                  30          


Glu Tyr Arg Val Gly Met Ile Pro Ala Ser Val Arg Glu Leu Ile Ser 
        35                  40                  45              


His Gly His Gln Val Phe Val Glu Thr Asn Ala Gly Ala Gly Ile Gly 
    50                  55                  60                  


Phe Ser Asp Asp Asp Tyr Ile Ala Val Gly Ala Ser Ile Leu Pro Thr 
65                  70                  75                  80  


Ala Ala Glu Val Phe Ala Gln Ala Asp Met Ile Val Lys Val Lys Glu 
                85                  90                  95      


Pro Gln Ala Val Glu Arg Ala Met Leu Lys Glu Gly Gln Ile Leu Phe 
            100                 105                 110         


Thr Tyr Leu His Leu Ala Pro Asp Phe Pro Gln Thr Glu Asp Leu Ile 
        115                 120                 125             


Lys Ser Lys Ala Val Cys Ile Ala Tyr Glu Thr Val Thr Asp Asn Met 
    130                 135                 140                 


Gly Arg Leu Pro Leu Leu Ala Pro Met Ser Glu Val Ala Gly Arg Met 
145                 150                 155                 160 


Ser Ile Gln Ala Gly Ala Gln Thr Leu Glu Lys Ser His Gly Gly Arg 
                165                 170                 175     


Gly Leu Leu Leu Gly Gly Val Pro Gly Val Glu Pro Ala Lys Val Val 
            180                 185                 190         


Ile Val Gly Gly Gly Val Val Gly Ala Asn Ala Ala Arg Met Ala Val 
        195                 200                 205             


Gly Met Arg Ala Asp Val Thr Ile Leu Asp Arg Asn Ile Asp Thr Leu 
    210                 215                 220                 


Arg Lys Leu Asp Glu Glu Phe Gln Gly Arg Ala Lys Val Val Tyr Ser 
225                 230                 235                 240 


Thr Glu Asp Ala Ile Glu Lys His Val Leu Ala Ala Asp Leu Val Ile 
                245                 250                 255     


Gly Ala Val Leu Ile Pro Gly Ala Ala Ala Pro Lys Leu Val Thr Lys 
            260                 265                 270         


Glu His Ile Ala Lys Met Lys Pro Gly Ala Ala Val Val Asp Val Ala 
        275                 280                 285             


Ile Asp Gln Gly Gly Cys Phe Glu Thr Ser His Ala Thr Thr His Ala 
    290                 295                 300                 


Asp Pro Thr Tyr Ile Val Asp Asp Val Val His Tyr Cys Val Ala Asn 
305                 310                 315                 320 


Met Pro Gly Ala Val Ala Arg Thr Ser Thr Phe Ala Leu Asn Asn Ala 
                325                 330                 335     


Thr Leu Pro Tyr Ile Val Lys Leu Ala Asn Lys Gly Tyr Arg Glu Ala 
            340                 345                 350         


Leu Leu Ala Asp His Gly Phe Leu Glu Gly Leu Asn Val Ile His Gly 
        355                 360                 365             


Lys Val Thr Cys Lys Glu Val Ala Glu Ala Phe Asn Leu Glu Tyr Val 
    370                 375                 380                 


Gln Pro Glu Thr Ala Ile Ala Met Phe Asn 
385                 390                 


<210>  46
<211>  31
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Forward primer

<400>  46
cgcgcggcag ccatatgatc attggcgtac c                                    31


<210>  47
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  PCR Reverse Primer

<400>  47
gtgcggccgc aagcttttag ttgaacatgg cg                                   32


<210>  48
<211>  1275
<212>  DNA
<213>  Bacillus subtilis


<220>
<221>  CDS
<222>  (1)..(1275)

<400>  48
atg tca gca aag caa gtc tcg aaa gat gaa gaa aaa gaa gct ctt aac       48
Met Ser Ala Lys Gln Val Ser Lys Asp Glu Glu Lys Glu Ala Leu Asn         
1               5                   10                  15              

tta ttt ctg tct acc caa aca atc att aag gaa gcc ctt cgg aag ctg       96
Leu Phe Leu Ser Thr Gln Thr Ile Ile Lys Glu Ala Leu Arg Lys Leu         
            20                  25                  30                  

ggt tat ccg gga gat atg tat gaa ctc atg aaa gag ccg cag aga atg      144
Gly Tyr Pro Gly Asp Met Tyr Glu Leu Met Lys Glu Pro Gln Arg Met         
        35                  40                  45                      

ctc act gtc cgc att ccg gtc aaa atg gac aat ggg agc gtc aaa gtg      192
Leu Thr Val Arg Ile Pro Val Lys Met Asp Asn Gly Ser Val Lys Val         
    50                  55                  60                          

ttc aca ggc tac cgg tca cag cac aat gat gct gtc ggt ccg aca aag      240
Phe Thr Gly Tyr Arg Ser Gln His Asn Asp Ala Val Gly Pro Thr Lys         
65                  70                  75                  80          

ggg ggc gtt cgc ttc cat cca gaa gtt aat gaa gag gaa gta aag gca      288
Gly Gly Val Arg Phe His Pro Glu Val Asn Glu Glu Glu Val Lys Ala         
                85                  90                  95              

tta tcc att tgg atg acg ctc aaa tgc ggg att gcc aat ctt cct tac      336
Leu Ser Ile Trp Met Thr Leu Lys Cys Gly Ile Ala Asn Leu Pro Tyr         
            100                 105                 110                 

ggc ggc ggg aag ggc ggt att att tgt gat ccg cgg aca atg tca ttt      384
Gly Gly Gly Lys Gly Gly Ile Ile Cys Asp Pro Arg Thr Met Ser Phe         
        115                 120                 125                     

gga gaa ctg gaa agg ctg agc agg ggg tat gtc cgt gcc atc agc cag      432
Gly Glu Leu Glu Arg Leu Ser Arg Gly Tyr Val Arg Ala Ile Ser Gln         
    130                 135                 140                         

atc gtc ggt ccg aca aag gat att cca gct ccc gat gtg tac acc aat      480
Ile Val Gly Pro Thr Lys Asp Ile Pro Ala Pro Asp Val Tyr Thr Asn         
145                 150                 155                 160         

tcg cag att atg gcg tgg atg atg gat gag tac agc cgg ctg cgg gaa      528
Ser Gln Ile Met Ala Trp Met Met Asp Glu Tyr Ser Arg Leu Arg Glu         
                165                 170                 175             

ttc gat tct ccg ggc ttt att aca ggt aaa ccg ctt gtt ttg gga gga      576
Phe Asp Ser Pro Gly Phe Ile Thr Gly Lys Pro Leu Val Leu Gly Gly         
            180                 185                 190                 

tcg caa gga cgg gaa aca gcg acg gca cag ggc gtc acg att tgt att      624
Ser Gln Gly Arg Glu Thr Ala Thr Ala Gln Gly Val Thr Ile Cys Ile         
        195                 200                 205                     

gaa gag gcg gtg aag aaa aaa ggg atc aag ctg caa aac gcg cgc atc      672
Glu Glu Ala Val Lys Lys Lys Gly Ile Lys Leu Gln Asn Ala Arg Ile         
    210                 215                 220                         

atc ata cag ggc ttt gga aac gcg ggt agc ttc ctg gcc aaa ttc atg      720
Ile Ile Gln Gly Phe Gly Asn Ala Gly Ser Phe Leu Ala Lys Phe Met         
225                 230                 235                 240         

cac gat gcg ggc gcg aag gtg atc ggg att tct gat gcc aat ggc ggg      768
His Asp Ala Gly Ala Lys Val Ile Gly Ile Ser Asp Ala Asn Gly Gly         
                245                 250                 255             

ctc tac aac cca gac ggc ctt gat atc cct tat ttg ctc gat aaa cgg      816
Leu Tyr Asn Pro Asp Gly Leu Asp Ile Pro Tyr Leu Leu Asp Lys Arg         
            260                 265                 270                 

gac agc ttt ggt atg gtc acc aat tta ttt act gac gtc atc aca aat      864
Asp Ser Phe Gly Met Val Thr Asn Leu Phe Thr Asp Val Ile Thr Asn         
        275                 280                 285                     

gag gag ctg ctt gaa aag gat tgc gat att tta gtg cct gcc gcg atc      912
Glu Glu Leu Leu Glu Lys Asp Cys Asp Ile Leu Val Pro Ala Ala Ile         
    290                 295                 300                         

tcc aat caa atc aca gcc aaa aac gca cat aac att cag gcg tca atc      960
Ser Asn Gln Ile Thr Ala Lys Asn Ala His Asn Ile Gln Ala Ser Ile         
305                 310                 315                 320         

gtc gtt gaa gcg gcg aac ggc ccg aca acc att gat gcc act aag atc     1008
Val Val Glu Ala Ala Asn Gly Pro Thr Thr Ile Asp Ala Thr Lys Ile         
                325                 330                 335             

ctg aat gaa aga ggc gtg ctg ctt gtg ccg gat atc cta gcg agt gcc     1056
Leu Asn Glu Arg Gly Val Leu Leu Val Pro Asp Ile Leu Ala Ser Ala         
            340                 345                 350                 

ggc ggc gtc acg gtt tct tat ttt gaa tgg gtg caa aac aac caa gga     1104
Gly Gly Val Thr Val Ser Tyr Phe Glu Trp Val Gln Asn Asn Gln Gly         
        355                 360                 365                     

tat tat tgg tcg gaa gaa gag gtt gca gaa aaa ctg aga agc gtc atg     1152
Tyr Tyr Trp Ser Glu Glu Glu Val Ala Glu Lys Leu Arg Ser Val Met         
    370                 375                 380                         

gtc agc tcg ttc gaa aca att tat caa aca gcg gca aca cat aaa gtg     1200
Val Ser Ser Phe Glu Thr Ile Tyr Gln Thr Ala Ala Thr His Lys Val         
385                 390                 395                 400         

gat atg cgt ttg gcg gct tac atg acg ggc atc aga aaa tcg gca gaa     1248
Asp Met Arg Leu Ala Ala Tyr Met Thr Gly Ile Arg Lys Ser Ala Glu         
                405                 410                 415             

gca tcg cgt ttc cgc gga tgg gtc taa                                 1275
Ala Ser Arg Phe Arg Gly Trp Val                                         
            420                                                         


<210>  49
<211>  424
<212>  PRT
<213>  Bacillus subtilis

<400>  49

Met Ser Ala Lys Gln Val Ser Lys Asp Glu Glu Lys Glu Ala Leu Asn 
1               5                   10                  15      


Leu Phe Leu Ser Thr Gln Thr Ile Ile Lys Glu Ala Leu Arg Lys Leu 
            20                  25                  30          


Gly Tyr Pro Gly Asp Met Tyr Glu Leu Met Lys Glu Pro Gln Arg Met 
        35                  40                  45              


Leu Thr Val Arg Ile Pro Val Lys Met Asp Asn Gly Ser Val Lys Val 
    50                  55                  60                  


Phe Thr Gly Tyr Arg Ser Gln His Asn Asp Ala Val Gly Pro Thr Lys 
65                  70                  75                  80  


Gly Gly Val Arg Phe His Pro Glu Val Asn Glu Glu Glu Val Lys Ala 
                85                  90                  95      


Leu Ser Ile Trp Met Thr Leu Lys Cys Gly Ile Ala Asn Leu Pro Tyr 
            100                 105                 110         


Gly Gly Gly Lys Gly Gly Ile Ile Cys Asp Pro Arg Thr Met Ser Phe 
        115                 120                 125             


Gly Glu Leu Glu Arg Leu Ser Arg Gly Tyr Val Arg Ala Ile Ser Gln 
    130                 135                 140                 


Ile Val Gly Pro Thr Lys Asp Ile Pro Ala Pro Asp Val Tyr Thr Asn 
145                 150                 155                 160 


Ser Gln Ile Met Ala Trp Met Met Asp Glu Tyr Ser Arg Leu Arg Glu 
                165                 170                 175     


Phe Asp Ser Pro Gly Phe Ile Thr Gly Lys Pro Leu Val Leu Gly Gly 
            180                 185                 190         


Ser Gln Gly Arg Glu Thr Ala Thr Ala Gln Gly Val Thr Ile Cys Ile 
        195                 200                 205             


Glu Glu Ala Val Lys Lys Lys Gly Ile Lys Leu Gln Asn Ala Arg Ile 
    210                 215                 220                 


Ile Ile Gln Gly Phe Gly Asn Ala Gly Ser Phe Leu Ala Lys Phe Met 
225                 230                 235                 240 


His Asp Ala Gly Ala Lys Val Ile Gly Ile Ser Asp Ala Asn Gly Gly 
                245                 250                 255     


Leu Tyr Asn Pro Asp Gly Leu Asp Ile Pro Tyr Leu Leu Asp Lys Arg 
            260                 265                 270         


Asp Ser Phe Gly Met Val Thr Asn Leu Phe Thr Asp Val Ile Thr Asn 
        275                 280                 285             


Glu Glu Leu Leu Glu Lys Asp Cys Asp Ile Leu Val Pro Ala Ala Ile 
    290                 295                 300                 


Ser Asn Gln Ile Thr Ala Lys Asn Ala His Asn Ile Gln Ala Ser Ile 
305                 310                 315                 320 


Val Val Glu Ala Ala Asn Gly Pro Thr Thr Ile Asp Ala Thr Lys Ile 
                325                 330                 335     


Leu Asn Glu Arg Gly Val Leu Leu Val Pro Asp Ile Leu Ala Ser Ala 
            340                 345                 350         


Gly Gly Val Thr Val Ser Tyr Phe Glu Trp Val Gln Asn Asn Gln Gly 
        355                 360                 365             


Tyr Tyr Trp Ser Glu Glu Glu Val Ala Glu Lys Leu Arg Ser Val Met 
    370                 375                 380                 


Val Ser Ser Phe Glu Thr Ile Tyr Gln Thr Ala Ala Thr His Lys Val 
385                 390                 395                 400 


Asp Met Arg Leu Ala Ala Tyr Met Thr Gly Ile Arg Lys Ser Ala Glu 
                405                 410                 415     


Ala Ser Arg Phe Arg Gly Trp Val 
            420                 


<210>  50
<211>  1266
<212>  DNA
<213>  Pyrobaculum islandicum


<220>
<221>  CDS
<222>  (1)..(1266)

<400>  50
atg gag agg aca ggg ttt tta gag tat gtg ctt aac tat gta aaa aaa       48
Met Glu Arg Thr Gly Phe Leu Glu Tyr Val Leu Asn Tyr Val Lys Lys         
1               5                   10                  15              

ggc gta gaa tta ggc gga ttt ccc gaa gat ttc tat aaa atc ttg tct       96
Gly Val Glu Leu Gly Gly Phe Pro Glu Asp Phe Tyr Lys Ile Leu Ser         
            20                  25                  30                  

agg cct agg cgt gtt ttg att gtt aat att cct gtg agg ttg gat ggt      144
Arg Pro Arg Arg Val Leu Ile Val Asn Ile Pro Val Arg Leu Asp Gly         
        35                  40                  45                      

ggt ggt ttt gag gtg ttt gag ggt tat cgt gtg cag cat tgt gat gtt      192
Gly Gly Phe Glu Val Phe Glu Gly Tyr Arg Val Gln His Cys Asp Val         
    50                  55                  60                          

ctc ggc ccg tat aag ggt ggg gtt cgt ttt cat cct gag gtt act ctt      240
Leu Gly Pro Tyr Lys Gly Gly Val Arg Phe His Pro Glu Val Thr Leu         
65                  70                  75                  80          

gcc gac gac gtc gcc ttg gct att tta atg acg ttg aaa aat agc cta      288
Ala Asp Asp Val Ala Leu Ala Ile Leu Met Thr Leu Lys Asn Ser Leu         
                85                  90                  95              

gct ggc ttg ccg tat ggc ggc gct aaa ggc gca gta cgt gta gac cct      336
Ala Gly Leu Pro Tyr Gly Gly Ala Lys Gly Ala Val Arg Val Asp Pro         
            100                 105                 110                 

aag aaa ctc tcg cag aga gaa ctt gaa gag ctc tct aga gga tat gcc      384
Lys Lys Leu Ser Gln Arg Glu Leu Glu Glu Leu Ser Arg Gly Tyr Ala         
        115                 120                 125                     

aga gcc ata gcc ccc ctc ata ggc gac gta gtc gac atc cca gcg cct      432
Arg Ala Ile Ala Pro Leu Ile Gly Asp Val Val Asp Ile Pro Ala Pro         
    130                 135                 140                         

gac gtt ggg aca aac gcc caa atc atg gcg tgg atg gta gac gaa tat      480
Asp Val Gly Thr Asn Ala Gln Ile Met Ala Trp Met Val Asp Glu Tyr         
145                 150                 155                 160         

tca aaa ata aag ggc tac aac gtg ccc ggg gta ttc acc tca aaa cca      528
Ser Lys Ile Lys Gly Tyr Asn Val Pro Gly Val Phe Thr Ser Lys Pro         
                165                 170                 175             

cca gag cta tgg gga aac cca gtg aga gaa tac gcc aca ggc ttt gga      576
Pro Glu Leu Trp Gly Asn Pro Val Arg Glu Tyr Ala Thr Gly Phe Gly         
            180                 185                 190                 

gta gca gta gcg aca aga gaa atg gca aaa aaa cta tgg ggc gga ata      624
Val Ala Val Ala Thr Arg Glu Met Ala Lys Lys Leu Trp Gly Gly Ile         
        195                 200                 205                     

gaa gga aaa aca gta gcg ata cag ggt atg ggt aat gtg ggg agg tgg      672
Glu Gly Lys Thr Val Ala Ile Gln Gly Met Gly Asn Val Gly Arg Trp         
    210                 215                 220                         

aca gcg tat tgg cta gaa aaa atg ggc gct aag gtg ata gct gtg tct      720
Thr Ala Tyr Trp Leu Glu Lys Met Gly Ala Lys Val Ile Ala Val Ser         
225                 230                 235                 240         

gat ata aat ggc gta gct tat aga aag gag gga ctt aat gtt gaa ttg      768
Asp Ile Asn Gly Val Ala Tyr Arg Lys Glu Gly Leu Asn Val Glu Leu         
                245                 250                 255             

ata caa aaa aac aag gga ctt aca ggg cct gct ctc gta gaa ctg ttt      816
Ile Gln Lys Asn Lys Gly Leu Thr Gly Pro Ala Leu Val Glu Leu Phe         
            260                 265                 270                 

acg aca aaa gac aat gcc gaa ttt gtt aaa aac cca gat gcc ata ttt      864
Thr Thr Lys Asp Asn Ala Glu Phe Val Lys Asn Pro Asp Ala Ile Phe         
        275                 280                 285                     

aaa cta gac gtc gac att ttc gtc ccc gcc gct att gag aat gtc att      912
Lys Leu Asp Val Asp Ile Phe Val Pro Ala Ala Ile Glu Asn Val Ile         
    290                 295                 300                         

agg ggt gat aat gct ggg ctt gtg aag gct agg ctt gtt gtt gag ggt      960
Arg Gly Asp Asn Ala Gly Leu Val Lys Ala Arg Leu Val Val Glu Gly         
305                 310                 315                 320         

gct aat ggc cct act act cct gag gct gag agg att ttg tat gag agg     1008
Ala Asn Gly Pro Thr Thr Pro Glu Ala Glu Arg Ile Leu Tyr Glu Arg         
                325                 330                 335             

ggg gtt gtc gta gtt cct gat atc ctc gcc aac gcc ggc ggc gtg att     1056
Gly Val Val Val Val Pro Asp Ile Leu Ala Asn Ala Gly Gly Val Ile         
            340                 345                 350                 

atg tcg tat tta gag tgg gtg gag aat ctc cag tgg tat atc tgg gac     1104
Met Ser Tyr Leu Glu Trp Val Glu Asn Leu Gln Trp Tyr Ile Trp Asp         
        355                 360                 365                     

gag gag gag act aga aag aga ctt gaa aac ata atg gta aac aac gtc     1152
Glu Glu Glu Thr Arg Lys Arg Leu Glu Asn Ile Met Val Asn Asn Val         
    370                 375                 380                         

gag aga gta tac aag aga tgg cag aga gaa aaa gga tgg aca atg aga     1200
Glu Arg Val Tyr Lys Arg Trp Gln Arg Glu Lys Gly Trp Thr Met Arg         
385                 390                 395                 400         

gac gca gca ata gta acc gca tta gaa aga ata tac aac gcc atg aaa     1248
Asp Ala Ala Ile Val Thr Ala Leu Glu Arg Ile Tyr Asn Ala Met Lys         
                405                 410                 415             

ata aga ggg tgg atc taa                                             1266
Ile Arg Gly Trp Ile                                                     
            420                                                         


<210>  51
<211>  421
<212>  PRT
<213>  Pyrobaculum islandicum

<400>  51

Met Glu Arg Thr Gly Phe Leu Glu Tyr Val Leu Asn Tyr Val Lys Lys 
1               5                   10                  15      


Gly Val Glu Leu Gly Gly Phe Pro Glu Asp Phe Tyr Lys Ile Leu Ser 
            20                  25                  30          


Arg Pro Arg Arg Val Leu Ile Val Asn Ile Pro Val Arg Leu Asp Gly 
        35                  40                  45              


Gly Gly Phe Glu Val Phe Glu Gly Tyr Arg Val Gln His Cys Asp Val 
    50                  55                  60                  


Leu Gly Pro Tyr Lys Gly Gly Val Arg Phe His Pro Glu Val Thr Leu 
65                  70                  75                  80  


Ala Asp Asp Val Ala Leu Ala Ile Leu Met Thr Leu Lys Asn Ser Leu 
                85                  90                  95      


Ala Gly Leu Pro Tyr Gly Gly Ala Lys Gly Ala Val Arg Val Asp Pro 
            100                 105                 110         


Lys Lys Leu Ser Gln Arg Glu Leu Glu Glu Leu Ser Arg Gly Tyr Ala 
        115                 120                 125             


Arg Ala Ile Ala Pro Leu Ile Gly Asp Val Val Asp Ile Pro Ala Pro 
    130                 135                 140                 


Asp Val Gly Thr Asn Ala Gln Ile Met Ala Trp Met Val Asp Glu Tyr 
145                 150                 155                 160 


Ser Lys Ile Lys Gly Tyr Asn Val Pro Gly Val Phe Thr Ser Lys Pro 
                165                 170                 175     


Pro Glu Leu Trp Gly Asn Pro Val Arg Glu Tyr Ala Thr Gly Phe Gly 
            180                 185                 190         


Val Ala Val Ala Thr Arg Glu Met Ala Lys Lys Leu Trp Gly Gly Ile 
        195                 200                 205             


Glu Gly Lys Thr Val Ala Ile Gln Gly Met Gly Asn Val Gly Arg Trp 
    210                 215                 220                 


Thr Ala Tyr Trp Leu Glu Lys Met Gly Ala Lys Val Ile Ala Val Ser 
225                 230                 235                 240 


Asp Ile Asn Gly Val Ala Tyr Arg Lys Glu Gly Leu Asn Val Glu Leu 
                245                 250                 255     


Ile Gln Lys Asn Lys Gly Leu Thr Gly Pro Ala Leu Val Glu Leu Phe 
            260                 265                 270         


Thr Thr Lys Asp Asn Ala Glu Phe Val Lys Asn Pro Asp Ala Ile Phe 
        275                 280                 285             


Lys Leu Asp Val Asp Ile Phe Val Pro Ala Ala Ile Glu Asn Val Ile 
    290                 295                 300                 


Arg Gly Asp Asn Ala Gly Leu Val Lys Ala Arg Leu Val Val Glu Gly 
305                 310                 315                 320 


Ala Asn Gly Pro Thr Thr Pro Glu Ala Glu Arg Ile Leu Tyr Glu Arg 
                325                 330                 335     


Gly Val Val Val Val Pro Asp Ile Leu Ala Asn Ala Gly Gly Val Ile 
            340                 345                 350         


Met Ser Tyr Leu Glu Trp Val Glu Asn Leu Gln Trp Tyr Ile Trp Asp 
        355                 360                 365             


Glu Glu Glu Thr Arg Lys Arg Leu Glu Asn Ile Met Val Asn Asn Val 
    370                 375                 380                 


Glu Arg Val Tyr Lys Arg Trp Gln Arg Glu Lys Gly Trp Thr Met Arg 
385                 390                 395                 400 


Asp Ala Ala Ile Val Thr Ala Leu Glu Arg Ile Tyr Asn Ala Met Lys 
                405                 410                 415     


Ile Arg Gly Trp Ile 
            420     


<210>  52
<211>  1353
<212>  DNA
<213>  Clostridium symbiosum


<220>
<221>  CDS
<222>  (1)..(1353)

<400>  52
atg agc aag tat gtt gac aga gta att gcg gaa gtc gag aag aaa tac       48
Met Ser Lys Tyr Val Asp Arg Val Ile Ala Glu Val Glu Lys Lys Tyr         
1               5                   10                  15              

gca gac gag ccg gag ttc gta caa aca gta gaa gag gtt tta tct tct       96
Ala Asp Glu Pro Glu Phe Val Gln Thr Val Glu Glu Val Leu Ser Ser         
            20                  25                  30                  

tta ggt ccg gtt gtt gat gca cat ccg gag tat gag gaa gta gct ctt      144
Leu Gly Pro Val Val Asp Ala His Pro Glu Tyr Glu Glu Val Ala Leu         
        35                  40                  45                      

ctc gag aga atg gta att ccg gag aga gtt atc gag ttc cgt gtt ccg      192
Leu Glu Arg Met Val Ile Pro Glu Arg Val Ile Glu Phe Arg Val Pro         
    50                  55                  60                          

tgg gag gat gac aac ggc aag gtt cat gta aac aca ggc tac cgc gtt      240
Trp Glu Asp Asp Asn Gly Lys Val His Val Asn Thr Gly Tyr Arg Val         
65                  70                  75                  80          

cag ttc aac ggc gcc att gga ccg tac aag ggc ggc tta cgt ttt gct      288
Gln Phe Asn Gly Ala Ile Gly Pro Tyr Lys Gly Gly Leu Arg Phe Ala         
                85                  90                  95              

ccg tct gta aac ctt tcc atc atg aaa ttc tta ggc ttt gag cag gca      336
Pro Ser Val Asn Leu Ser Ile Met Lys Phe Leu Gly Phe Glu Gln Ala         
            100                 105                 110                 

ttc aaa gac agc ctt aca aca ctt cct atg ggt ggt gcc aaa ggt ggt      384
Phe Lys Asp Ser Leu Thr Thr Leu Pro Met Gly Gly Ala Lys Gly Gly         
        115                 120                 125                     

tcc gac ttc gat ccg aac ggt aag agc gac aga gaa gtt atg aga ttc      432
Ser Asp Phe Asp Pro Asn Gly Lys Ser Asp Arg Glu Val Met Arg Phe         
    130                 135                 140                         

tgc cag gct ttc atg aca gaa tta tac cgt cac atc gga ccg gac att      480
Cys Gln Ala Phe Met Thr Glu Leu Tyr Arg His Ile Gly Pro Asp Ile         
145                 150                 155                 160         

gat gtt cct gca ggt gac ctt ggt gta ggc gct cgt gaa atc ggt tat      528
Asp Val Pro Ala Gly Asp Leu Gly Val Gly Ala Arg Glu Ile Gly Tyr         
                165                 170                 175             

atg tac gga cag tat cgt aaa att gtc ggc gga ttc tac aac ggc gtt      576
Met Tyr Gly Gln Tyr Arg Lys Ile Val Gly Gly Phe Tyr Asn Gly Val         
            180                 185                 190                 

ctg aca ggt aag gca aga tcc ttc ggt ggt tcc tta gtt cgt ccg gaa      624
Leu Thr Gly Lys Ala Arg Ser Phe Gly Gly Ser Leu Val Arg Pro Glu         
        195                 200                 205                     

gct aca gga tac ggc tcc gta tac tat gta gaa gca gtt atg aaa cat      672
Ala Thr Gly Tyr Gly Ser Val Tyr Tyr Val Glu Ala Val Met Lys His         
    210                 215                 220                         

gag aac gat acc ctg gtt ggc aag aca gtt gca tta gca ggt ttt ggt      720
Glu Asn Asp Thr Leu Val Gly Lys Thr Val Ala Leu Ala Gly Phe Gly         
225                 230                 235                 240         

aac gta gca tgg ggc gct gct aag aag ctt gct gaa tta ggc gct aaa      768
Asn Val Ala Trp Gly Ala Ala Lys Lys Leu Ala Glu Leu Gly Ala Lys         
                245                 250                 255             

gca gtt aca ctt tcc gga cca gac gga tac atc tac gat ccg gaa ggc      816
Ala Val Thr Leu Ser Gly Pro Asp Gly Tyr Ile Tyr Asp Pro Glu Gly         
            260                 265                 270                 

atc aca aca gaa gag aag atc aac tac atg ctt gag atg aga gca tcc      864
Ile Thr Thr Glu Glu Lys Ile Asn Tyr Met Leu Glu Met Arg Ala Ser         
        275                 280                 285                     

gga cgt aac aaa gtt cag gat tat gca gac aag ttc ggc gta cag ttc      912
Gly Arg Asn Lys Val Gln Asp Tyr Ala Asp Lys Phe Gly Val Gln Phe         
    290                 295                 300                         

ttc ccg ggt gag aaa cca tgg ggc cag aag gtt gac atc atc atg cca      960
Phe Pro Gly Glu Lys Pro Trp Gly Gln Lys Val Asp Ile Ile Met Pro         
305                 310                 315                 320         

tgc gct act cag aac gat gtt gat tta gag cag gct aag aag atc gtt     1008
Cys Ala Thr Gln Asn Asp Val Asp Leu Glu Gln Ala Lys Lys Ile Val         
                325                 330                 335             

gct aac aac gtt aaa tac tac atc gaa gtt gct aac atg cca act acc     1056
Ala Asn Asn Val Lys Tyr Tyr Ile Glu Val Ala Asn Met Pro Thr Thr         
            340                 345                 350                 

aac gaa gct ctc aga ttc tta atg cag cag cca aac atg gta gta gct     1104
Asn Glu Ala Leu Arg Phe Leu Met Gln Gln Pro Asn Met Val Val Ala         
        355                 360                 365                     

cct tcc aag gct gta aac gca ggc ggc gtt ctt gta tcc ggc ttt gag     1152
Pro Ser Lys Ala Val Asn Ala Gly Gly Val Leu Val Ser Gly Phe Glu         
    370                 375                 380                         

atg agc cag aac agc gag aga ctt tcc tgg aca gca gag gaa gtt gac     1200
Met Ser Gln Asn Ser Glu Arg Leu Ser Trp Thr Ala Glu Glu Val Asp         
385                 390                 395                 400         

tcc aaa ctt cat cag gtt atg aca gat atc cat gac ggt tcc gct gca     1248
Ser Lys Leu His Gln Val Met Thr Asp Ile His Asp Gly Ser Ala Ala         
                405                 410                 415             

gca gca gag cgt tac ggc tta ggc tac aat ctg gtt gcc ggc gct aac     1296
Ala Ala Glu Arg Tyr Gly Leu Gly Tyr Asn Leu Val Ala Gly Ala Asn         
            420                 425                 430                 

atc gtt gga ttc cag aag att gct gat gct atg atg gct cag ggc att     1344
Ile Val Gly Phe Gln Lys Ile Ala Asp Ala Met Met Ala Gln Gly Ile         
        435                 440                 445                     

gct tgg taa                                                         1353
Ala Trp                                                                 
    450                                                                 


<210>  53
<211>  450
<212>  PRT
<213>  Clostridium symbiosum

<400>  53

Met Ser Lys Tyr Val Asp Arg Val Ile Ala Glu Val Glu Lys Lys Tyr 
1               5                   10                  15      


Ala Asp Glu Pro Glu Phe Val Gln Thr Val Glu Glu Val Leu Ser Ser 
            20                  25                  30          


Leu Gly Pro Val Val Asp Ala His Pro Glu Tyr Glu Glu Val Ala Leu 
        35                  40                  45              


Leu Glu Arg Met Val Ile Pro Glu Arg Val Ile Glu Phe Arg Val Pro 
    50                  55                  60                  


Trp Glu Asp Asp Asn Gly Lys Val His Val Asn Thr Gly Tyr Arg Val 
65                  70                  75                  80  


Gln Phe Asn Gly Ala Ile Gly Pro Tyr Lys Gly Gly Leu Arg Phe Ala 
                85                  90                  95      


Pro Ser Val Asn Leu Ser Ile Met Lys Phe Leu Gly Phe Glu Gln Ala 
            100                 105                 110         


Phe Lys Asp Ser Leu Thr Thr Leu Pro Met Gly Gly Ala Lys Gly Gly 
        115                 120                 125             


Ser Asp Phe Asp Pro Asn Gly Lys Ser Asp Arg Glu Val Met Arg Phe 
    130                 135                 140                 


Cys Gln Ala Phe Met Thr Glu Leu Tyr Arg His Ile Gly Pro Asp Ile 
145                 150                 155                 160 


Asp Val Pro Ala Gly Asp Leu Gly Val Gly Ala Arg Glu Ile Gly Tyr 
                165                 170                 175     


Met Tyr Gly Gln Tyr Arg Lys Ile Val Gly Gly Phe Tyr Asn Gly Val 
            180                 185                 190         


Leu Thr Gly Lys Ala Arg Ser Phe Gly Gly Ser Leu Val Arg Pro Glu 
        195                 200                 205             


Ala Thr Gly Tyr Gly Ser Val Tyr Tyr Val Glu Ala Val Met Lys His 
    210                 215                 220                 


Glu Asn Asp Thr Leu Val Gly Lys Thr Val Ala Leu Ala Gly Phe Gly 
225                 230                 235                 240 


Asn Val Ala Trp Gly Ala Ala Lys Lys Leu Ala Glu Leu Gly Ala Lys 
                245                 250                 255     


Ala Val Thr Leu Ser Gly Pro Asp Gly Tyr Ile Tyr Asp Pro Glu Gly 
            260                 265                 270         


Ile Thr Thr Glu Glu Lys Ile Asn Tyr Met Leu Glu Met Arg Ala Ser 
        275                 280                 285             


Gly Arg Asn Lys Val Gln Asp Tyr Ala Asp Lys Phe Gly Val Gln Phe 
    290                 295                 300                 


Phe Pro Gly Glu Lys Pro Trp Gly Gln Lys Val Asp Ile Ile Met Pro 
305                 310                 315                 320 


Cys Ala Thr Gln Asn Asp Val Asp Leu Glu Gln Ala Lys Lys Ile Val 
                325                 330                 335     


Ala Asn Asn Val Lys Tyr Tyr Ile Glu Val Ala Asn Met Pro Thr Thr 
            340                 345                 350         


Asn Glu Ala Leu Arg Phe Leu Met Gln Gln Pro Asn Met Val Val Ala 
        355                 360                 365             


Pro Ser Lys Ala Val Asn Ala Gly Gly Val Leu Val Ser Gly Phe Glu 
    370                 375                 380                 


Met Ser Gln Asn Ser Glu Arg Leu Ser Trp Thr Ala Glu Glu Val Asp 
385                 390                 395                 400 


Ser Lys Leu His Gln Val Met Thr Asp Ile His Asp Gly Ser Ala Ala 
                405                 410                 415     


Ala Ala Glu Arg Tyr Gly Leu Gly Tyr Asn Leu Val Ala Gly Ala Asn 
            420                 425                 430         


Ile Val Gly Phe Gln Lys Ile Ala Asp Ala Met Met Ala Gln Gly Ile 
        435                 440                 445             


Ala Trp 
    450 


<210>  54
<211>  1257
<212>  DNA
<213>  Thermococcus profundus


<220>
<221>  CDS
<222>  (1)..(1257)

<400>  54
atg gtc gag att gac ccg ttt gag atg gcc gtt aag cag ctt gag agg       48
Met Val Glu Ile Asp Pro Phe Glu Met Ala Val Lys Gln Leu Glu Arg         
1               5                   10                  15              

gct gct cag tac atg gac ata agc gaa gag gcc ctc gag tgg ctt aag       96
Ala Ala Gln Tyr Met Asp Ile Ser Glu Glu Ala Leu Glu Trp Leu Lys         
            20                  25                  30                  

aag ccc atg agg atc gtc gaa gtt agc gtt ccg ata gag atg gac gac      144
Lys Pro Met Arg Ile Val Glu Val Ser Val Pro Ile Glu Met Asp Asp         
        35                  40                  45                      

ggt tct gtt aag gtt ttc acc ggt ttc cgc gtt cag cac aac tgg gct      192
Gly Ser Val Lys Val Phe Thr Gly Phe Arg Val Gln His Asn Trp Ala         
    50                  55                  60                          

cgc ggc ccg acc aag ggt ggt ata agg tgg cac ccg gcc gag acc ctc      240
Arg Gly Pro Thr Lys Gly Gly Ile Arg Trp His Pro Ala Glu Thr Leu         
65                  70                  75                  80          

agc acc gtt aag gcc ctc gct acc tgg atg acc tgg aag gtt gca gtt      288
Ser Thr Val Lys Ala Leu Ala Thr Trp Met Thr Trp Lys Val Ala Val         
                85                  90                  95              

gtc gac ctc ccc tac ggt gga ggt aag ggt ggc atc atc gtc aac ccg      336
Val Asp Leu Pro Tyr Gly Gly Gly Lys Gly Gly Ile Ile Val Asn Pro         
            100                 105                 110                 

aag gag ctc tcc gag agg gag cag gag agg ctc gct cgc gct tac ata      384
Lys Glu Leu Ser Glu Arg Glu Gln Glu Arg Leu Ala Arg Ala Tyr Ile         
        115                 120                 125                     

agg gcc gtt tat gac gtc atc ggt ccg tgg acc gac att ccg gct ccc      432
Arg Ala Val Tyr Asp Val Ile Gly Pro Trp Thr Asp Ile Pro Ala Pro         
    130                 135                 140                         

gac gtc tac acc aac ccg aag atc atg ggc tgg atg atg gac gag tat      480
Asp Val Tyr Thr Asn Pro Lys Ile Met Gly Trp Met Met Asp Glu Tyr         
145                 150                 155                 160         

gaa aca ata atg agg agg aag ggc ccg gcc ttc ggt gtc atc act gga      528
Glu Thr Ile Met Arg Arg Lys Gly Pro Ala Phe Gly Val Ile Thr Gly         
                165                 170                 175             

aag ccg ctc agc att ggc ggt tca ctc ggt agg ggt aca gca aca gcc      576
Lys Pro Leu Ser Ile Gly Gly Ser Leu Gly Arg Gly Thr Ala Thr Ala         
            180                 185                 190                 

cag ggt gca ata ttc acc atc agg gag gcc gcc aag gcc ctc ggc atc      624
Gln Gly Ala Ile Phe Thr Ile Arg Glu Ala Ala Lys Ala Leu Gly Ile         
        195                 200                 205                     

gac ctc aag ggc aag aag atc gcc gtc cag ggc tat ggt aac gcc ggc      672
Asp Leu Lys Gly Lys Lys Ile Ala Val Gln Gly Tyr Gly Asn Ala Gly         
    210                 215                 220                         

tac tac acc gcc aag ctc gcc aag gag cag ctc ggc atg acc gtc gtc      720
Tyr Tyr Thr Ala Lys Leu Ala Lys Glu Gln Leu Gly Met Thr Val Val         
225                 230                 235                 240         

gcc gtc agc gac agc cgc ggc ggc atc tac aac ccg gac ggc ctc gac      768
Ala Val Ser Asp Ser Arg Gly Gly Ile Tyr Asn Pro Asp Gly Leu Asp         
                245                 250                 255             

ccg gac gag gtt ctc aag tgg aag agg gag cac ggc tca gtt aag gac      816
Pro Asp Glu Val Leu Lys Trp Lys Arg Glu His Gly Ser Val Lys Asp         
            260                 265                 270                 

ttc cca gga gcg acc aac atc acc aac gag gag ctc ctc gag ctc gag      864
Phe Pro Gly Ala Thr Asn Ile Thr Asn Glu Glu Leu Leu Glu Leu Glu         
        275                 280                 285                     

gta gac gtc ctc gct ccg gca gct atc gaa gag gtc atc acc gag aag      912
Val Asp Val Leu Ala Pro Ala Ala Ile Glu Glu Val Ile Thr Glu Lys         
    290                 295                 300                         

aac gcc gac aac atc aag gcc aag atc gtc gcc gag gtt gcc aac ggt      960
Asn Ala Asp Asn Ile Lys Ala Lys Ile Val Ala Glu Val Ala Asn Gly         
305                 310                 315                 320         

cca gtt acg cca gag gca gac gac atc ctc cgc gag aag ggc atc ctc     1008
Pro Val Thr Pro Glu Ala Asp Asp Ile Leu Arg Glu Lys Gly Ile Leu         
                325                 330                 335             

cag att ccg gac ttc ctg tgc aac gcc ggt ggc gtc acc gtc agc tac     1056
Gln Ile Pro Asp Phe Leu Cys Asn Ala Gly Gly Val Thr Val Ser Tyr         
            340                 345                 350                 

ttc gag tgg gtc cag aac ata aac ggc tac tac tgg acc gag gag gag     1104
Phe Glu Trp Val Gln Asn Ile Asn Gly Tyr Tyr Trp Thr Glu Glu Glu         
        355                 360                 365                     

gtc cgc gag aag ctc gac aag aag atg acc aag gcc ttc tgg gag gtc     1152
Val Arg Glu Lys Leu Asp Lys Lys Met Thr Lys Ala Phe Trp Glu Val         
    370                 375                 380                         

tac aac acc cac aag gac aag aac atc cac atg agg gac gcc gcc tac     1200
Tyr Asn Thr His Lys Asp Lys Asn Ile His Met Arg Asp Ala Ala Tyr         
385                 390                 395                 400         

gtc gtc gcg gtc agc agg gtt tac cag gcc atg aag gac cgc gga tgg     1248
Val Val Ala Val Ser Arg Val Tyr Gln Ala Met Lys Asp Arg Gly Trp         
                405                 410                 415             

gtg aag aag                                                         1257
Val Lys Lys                                                             
                                                                        


<210>  55
<211>  419
<212>  PRT
<213>  Thermococcus profundus

<400>  55

Met Val Glu Ile Asp Pro Phe Glu Met Ala Val Lys Gln Leu Glu Arg 
1               5                   10                  15      


Ala Ala Gln Tyr Met Asp Ile Ser Glu Glu Ala Leu Glu Trp Leu Lys 
            20                  25                  30          


Lys Pro Met Arg Ile Val Glu Val Ser Val Pro Ile Glu Met Asp Asp 
        35                  40                  45              


Gly Ser Val Lys Val Phe Thr Gly Phe Arg Val Gln His Asn Trp Ala 
    50                  55                  60                  


Arg Gly Pro Thr Lys Gly Gly Ile Arg Trp His Pro Ala Glu Thr Leu 
65                  70                  75                  80  


Ser Thr Val Lys Ala Leu Ala Thr Trp Met Thr Trp Lys Val Ala Val 
                85                  90                  95      


Val Asp Leu Pro Tyr Gly Gly Gly Lys Gly Gly Ile Ile Val Asn Pro 
            100                 105                 110         


Lys Glu Leu Ser Glu Arg Glu Gln Glu Arg Leu Ala Arg Ala Tyr Ile 
        115                 120                 125             


Arg Ala Val Tyr Asp Val Ile Gly Pro Trp Thr Asp Ile Pro Ala Pro 
    130                 135                 140                 


Asp Val Tyr Thr Asn Pro Lys Ile Met Gly Trp Met Met Asp Glu Tyr 
145                 150                 155                 160 


Glu Thr Ile Met Arg Arg Lys Gly Pro Ala Phe Gly Val Ile Thr Gly 
                165                 170                 175     


Lys Pro Leu Ser Ile Gly Gly Ser Leu Gly Arg Gly Thr Ala Thr Ala 
            180                 185                 190         


Gln Gly Ala Ile Phe Thr Ile Arg Glu Ala Ala Lys Ala Leu Gly Ile 
        195                 200                 205             


Asp Leu Lys Gly Lys Lys Ile Ala Val Gln Gly Tyr Gly Asn Ala Gly 
    210                 215                 220                 


Tyr Tyr Thr Ala Lys Leu Ala Lys Glu Gln Leu Gly Met Thr Val Val 
225                 230                 235                 240 


Ala Val Ser Asp Ser Arg Gly Gly Ile Tyr Asn Pro Asp Gly Leu Asp 
                245                 250                 255     


Pro Asp Glu Val Leu Lys Trp Lys Arg Glu His Gly Ser Val Lys Asp 
            260                 265                 270         


Phe Pro Gly Ala Thr Asn Ile Thr Asn Glu Glu Leu Leu Glu Leu Glu 
        275                 280                 285             


Val Asp Val Leu Ala Pro Ala Ala Ile Glu Glu Val Ile Thr Glu Lys 
    290                 295                 300                 


Asn Ala Asp Asn Ile Lys Ala Lys Ile Val Ala Glu Val Ala Asn Gly 
305                 310                 315                 320 


Pro Val Thr Pro Glu Ala Asp Asp Ile Leu Arg Glu Lys Gly Ile Leu 
                325                 330                 335     


Gln Ile Pro Asp Phe Leu Cys Asn Ala Gly Gly Val Thr Val Ser Tyr 
            340                 345                 350         


Phe Glu Trp Val Gln Asn Ile Asn Gly Tyr Tyr Trp Thr Glu Glu Glu 
        355                 360                 365             


Val Arg Glu Lys Leu Asp Lys Lys Met Thr Lys Ala Phe Trp Glu Val 
    370                 375                 380                 


Tyr Asn Thr His Lys Asp Lys Asn Ile His Met Arg Asp Ala Ala Tyr 
385                 390                 395                 400 


Val Val Ala Val Ser Arg Val Tyr Gln Ala Met Lys Asp Arg Gly Trp 
                405                 410                 415     


Val Lys Lys 
            


<210>  56
<211>  1380
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(1380)

<400>  56
atg aac agg tta cct tcg agc gca tcg gct tta gcg tgc agc gcc cac       48
Met Asn Arg Leu Pro Ser Ser Ala Ser Ala Leu Ala Cys Ser Ala His         
1               5                   10                  15              

gcc ctg aat ctc att gag aag cga acg ctg gat cat gag gag atg aaa       96
Ala Leu Asn Leu Ile Glu Lys Arg Thr Leu Asp His Glu Glu Met Lys         
            20                  25                  30                  

gca ctt aac cga gag gtg att gaa tac ttc aaa gag cat gtc aat ccg      144
Ala Leu Asn Arg Glu Val Ile Glu Tyr Phe Lys Glu His Val Asn Pro         
        35                  40                  45                      

ggg ttt tta gag tat cgc aaa tct gtt acc gcc ggc ggg gat tac gga      192
Gly Phe Leu Glu Tyr Arg Lys Ser Val Thr Ala Gly Gly Asp Tyr Gly         
    50                  55                  60                          

gcc gta gag tgg caa gcg gga agt tta aat acg ctt gtc gac acc cag      240
Ala Val Glu Trp Gln Ala Gly Ser Leu Asn Thr Leu Val Asp Thr Gln         
65                  70                  75                  80          

ggc cag gag ttt atc gac tgc ctg gga ggt ttt gga att ttc aac gtg      288
Gly Gln Glu Phe Ile Asp Cys Leu Gly Gly Phe Gly Ile Phe Asn Val         
                85                  90                  95              

ggg cac cgt aat cca gtt gtg gtt tcc gcc gta cag aat caa ctt gcg      336
Gly His Arg Asn Pro Val Val Val Ser Ala Val Gln Asn Gln Leu Ala         
            100                 105                 110                 

aaa caa ccg ctg cac agc cag gag ctg ctc gat ccg tta cgg gcg atg      384
Lys Gln Pro Leu His Ser Gln Glu Leu Leu Asp Pro Leu Arg Ala Met         
        115                 120                 125                     

ttg gcg aaa acc ctt gct gcg cta acg ccc ggt aaa ctg aaa tac agc      432
Leu Ala Lys Thr Leu Ala Ala Leu Thr Pro Gly Lys Leu Lys Tyr Ser         
    130                 135                 140                         

ttc ttc tgt aat agc ggc acc gag tcc gtt gaa gca gcg ctg aag ctg      480
Phe Phe Cys Asn Ser Gly Thr Glu Ser Val Glu Ala Ala Leu Lys Leu         
145                 150                 155                 160         

gcg aaa gct tac cag tca ccg cgc ggc aag ttt act ttt att gcc acc      528
Ala Lys Ala Tyr Gln Ser Pro Arg Gly Lys Phe Thr Phe Ile Ala Thr         
                165                 170                 175             

agc ggc gcg ttc cac ggt aaa tca ctt ggc gcg ctg tcg gcc acg gcg      576
Ser Gly Ala Phe His Gly Lys Ser Leu Gly Ala Leu Ser Ala Thr Ala         
            180                 185                 190                 

aaa tcg acc ttc cgc aaa ccg ttt atg ccg tta ctg ccg ggc ttc cgt      624
Lys Ser Thr Phe Arg Lys Pro Phe Met Pro Leu Leu Pro Gly Phe Arg         
        195                 200                 205                     

cat gtg ccg ttt ggc aat atc gaa gcc atg cgc acg gct ctt aac gag      672
His Val Pro Phe Gly Asn Ile Glu Ala Met Arg Thr Ala Leu Asn Glu         
    210                 215                 220                         

tgc aaa aaa acc ggt gat gat gtg gct gcg gtg atc ctc gaa ccg att      720
Cys Lys Lys Thr Gly Asp Asp Val Ala Ala Val Ile Leu Glu Pro Ile         
225                 230                 235                 240         

cag ggt gaa ggt ggc gta att ctg ccg ccg ccg ggc tat ctc acc gcc      768
Gln Gly Glu Gly Gly Val Ile Leu Pro Pro Pro Gly Tyr Leu Thr Ala         
                245                 250                 255             

gta cgt aag cta tgc gat gag ttc ggc gca ctg atg atc ctc gat gaa      816
Val Arg Lys Leu Cys Asp Glu Phe Gly Ala Leu Met Ile Leu Asp Glu         
            260                 265                 270                 

gta caa acg ggc atg ggg cgc acg ggc aag atg ttc gcc tgc gag cat      864
Val Gln Thr Gly Met Gly Arg Thr Gly Lys Met Phe Ala Cys Glu His         
        275                 280                 285                     

gag aac gta cag ccg gat atc ctc tgc ctt gcc aaa gcg ctc ggc ggc      912
Glu Asn Val Gln Pro Asp Ile Leu Cys Leu Ala Lys Ala Leu Gly Gly         
    290                 295                 300                         

ggc gtg atg ccg att ggc gcg acc atc gcc act gaa gag gtg ttt tca      960
Gly Val Met Pro Ile Gly Ala Thr Ile Ala Thr Glu Glu Val Phe Ser         
305                 310                 315                 320         

gtt ctg ttc gac aac cca ttc ctg cat acc acc acc ttt ggc ggc aac     1008
Val Leu Phe Asp Asn Pro Phe Leu His Thr Thr Thr Phe Gly Gly Asn         
                325                 330                 335             

ccg ctg gcc tgt gcg gcg gcg ctg gcg acc atc aat gtg ttg ctg gag     1056
Pro Leu Ala Cys Ala Ala Ala Leu Ala Thr Ile Asn Val Leu Leu Glu         
            340                 345                 350                 

cag aac tta ccg gct cag gct gag caa aaa ggc gat atg ttg ctg gac     1104
Gln Asn Leu Pro Ala Gln Ala Glu Gln Lys Gly Asp Met Leu Leu Asp         
        355                 360                 365                     

ggt ttc cgt caa ctg gcg cgg gaa tat ccc gat ctg gta cag gaa gcg     1152
Gly Phe Arg Gln Leu Ala Arg Glu Tyr Pro Asp Leu Val Gln Glu Ala         
    370                 375                 380                         

cgt ggt aaa ggg atg ttg atg gcg att gag ttt gtt gat aac gaa atc     1200
Arg Gly Lys Gly Met Leu Met Ala Ile Glu Phe Val Asp Asn Glu Ile         
385                 390                 395                 400         

ggc tat aac ttt gcc agc gag atg ttc cgc cag cgc gta ctg gtg gcc     1248
Gly Tyr Asn Phe Ala Ser Glu Met Phe Arg Gln Arg Val Leu Val Ala         
                405                 410                 415             

gga acg ctc aat aac gcc aaa acg atc cgc att gaa ccg cca ctg aca     1296
Gly Thr Leu Asn Asn Ala Lys Thr Ile Arg Ile Glu Pro Pro Leu Thr         
            420                 425                 430                 

ctg acc att gaa cag tgt gaa ctg gtg atc aaa gcg gcg cgt aag gcg     1344
Leu Thr Ile Glu Gln Cys Glu Leu Val Ile Lys Ala Ala Arg Lys Ala         
        435                 440                 445                     

ctg gcg gcc atg cga gta agt gtc gaa gaa gcg taa                     1380
Leu Ala Ala Met Arg Val Ser Val Glu Glu Ala                             
    450                 455                                             


<210>  57
<211>  459
<212>  PRT
<213>  Escherichia coli

<400>  57

Met Asn Arg Leu Pro Ser Ser Ala Ser Ala Leu Ala Cys Ser Ala His 
1               5                   10                  15      


Ala Leu Asn Leu Ile Glu Lys Arg Thr Leu Asp His Glu Glu Met Lys 
            20                  25                  30          


Ala Leu Asn Arg Glu Val Ile Glu Tyr Phe Lys Glu His Val Asn Pro 
        35                  40                  45              


Gly Phe Leu Glu Tyr Arg Lys Ser Val Thr Ala Gly Gly Asp Tyr Gly 
    50                  55                  60                  


Ala Val Glu Trp Gln Ala Gly Ser Leu Asn Thr Leu Val Asp Thr Gln 
65                  70                  75                  80  


Gly Gln Glu Phe Ile Asp Cys Leu Gly Gly Phe Gly Ile Phe Asn Val 
                85                  90                  95      


Gly His Arg Asn Pro Val Val Val Ser Ala Val Gln Asn Gln Leu Ala 
            100                 105                 110         


Lys Gln Pro Leu His Ser Gln Glu Leu Leu Asp Pro Leu Arg Ala Met 
        115                 120                 125             


Leu Ala Lys Thr Leu Ala Ala Leu Thr Pro Gly Lys Leu Lys Tyr Ser 
    130                 135                 140                 


Phe Phe Cys Asn Ser Gly Thr Glu Ser Val Glu Ala Ala Leu Lys Leu 
145                 150                 155                 160 


Ala Lys Ala Tyr Gln Ser Pro Arg Gly Lys Phe Thr Phe Ile Ala Thr 
                165                 170                 175     


Ser Gly Ala Phe His Gly Lys Ser Leu Gly Ala Leu Ser Ala Thr Ala 
            180                 185                 190         


Lys Ser Thr Phe Arg Lys Pro Phe Met Pro Leu Leu Pro Gly Phe Arg 
        195                 200                 205             


His Val Pro Phe Gly Asn Ile Glu Ala Met Arg Thr Ala Leu Asn Glu 
    210                 215                 220                 


Cys Lys Lys Thr Gly Asp Asp Val Ala Ala Val Ile Leu Glu Pro Ile 
225                 230                 235                 240 


Gln Gly Glu Gly Gly Val Ile Leu Pro Pro Pro Gly Tyr Leu Thr Ala 
                245                 250                 255     


Val Arg Lys Leu Cys Asp Glu Phe Gly Ala Leu Met Ile Leu Asp Glu 
            260                 265                 270         


Val Gln Thr Gly Met Gly Arg Thr Gly Lys Met Phe Ala Cys Glu His 
        275                 280                 285             


Glu Asn Val Gln Pro Asp Ile Leu Cys Leu Ala Lys Ala Leu Gly Gly 
    290                 295                 300                 


Gly Val Met Pro Ile Gly Ala Thr Ile Ala Thr Glu Glu Val Phe Ser 
305                 310                 315                 320 


Val Leu Phe Asp Asn Pro Phe Leu His Thr Thr Thr Phe Gly Gly Asn 
                325                 330                 335     


Pro Leu Ala Cys Ala Ala Ala Leu Ala Thr Ile Asn Val Leu Leu Glu 
            340                 345                 350         


Gln Asn Leu Pro Ala Gln Ala Glu Gln Lys Gly Asp Met Leu Leu Asp 
        355                 360                 365             


Gly Phe Arg Gln Leu Ala Arg Glu Tyr Pro Asp Leu Val Gln Glu Ala 
    370                 375                 380                 


Arg Gly Lys Gly Met Leu Met Ala Ile Glu Phe Val Asp Asn Glu Ile 
385                 390                 395                 400 


Gly Tyr Asn Phe Ala Ser Glu Met Phe Arg Gln Arg Val Leu Val Ala 
                405                 410                 415     


Gly Thr Leu Asn Asn Ala Lys Thr Ile Arg Ile Glu Pro Pro Leu Thr 
            420                 425                 430         


Leu Thr Ile Glu Gln Cys Glu Leu Val Ile Lys Ala Ala Arg Lys Ala 
        435                 440                 445             


Leu Ala Ala Met Arg Val Ser Val Glu Glu Ala 
    450                 455                 


<210>  58
<211>  34
<212>  DNA
<213>  artificial

<220>
<223>  PCR primer

<400>  58
gcgcgcatat gaacaggtta ccttcgagcg catc                                 34


<210>  59
<211>  35
<212>  DNA
<213>  Artificial

<220>
<223>  PCR primer

<400>  59
cgcgcgctcg agttacgctt cttcgacact tactc                                35


<210>  60
<211>  1323
<212>  DNA
<213>  Achromobacter denitrificans


<220>
<221>  CDS
<222>  (1)..(1323)

<400>  60
atg agc gct gcc aaa ctg ccc gac ctg tcc cac ctc tgg atg ccc ttt       48
Met Ser Ala Ala Lys Leu Pro Asp Leu Ser His Leu Trp Met Pro Phe         
1               5                   10                  15              

acc gcc aac cgg cag ttc aag gcg aac ccc cgc ctg ctg gcc tcg gcc       96
Thr Ala Asn Arg Gln Phe Lys Ala Asn Pro Arg Leu Leu Ala Ser Ala         
            20                  25                  30                  

aag ggc atg tac tac acg tct ttc gac ggc cgc cag atc ctg gac ggc      144
Lys Gly Met Tyr Tyr Thr Ser Phe Asp Gly Arg Gln Ile Leu Asp Gly         
        35                  40                  45                      

acg gcc ggc ctg tgg tgc gtg aac gcc ggc cac tgc cgc gaa gaa atc      192
Thr Ala Gly Leu Trp Cys Val Asn Ala Gly His Cys Arg Glu Glu Ile         
    50                  55                  60                          

gtc tcc gcc atc gcc agc cag gcc ggc gtc atg gac tac gcg ccg ggg      240
Val Ser Ala Ile Ala Ser Gln Ala Gly Val Met Asp Tyr Ala Pro Gly         
65                  70                  75                  80          

ttc cag ctc ggc cac ccg ctg gcc ttc gag gcc gcc acc gcc gtg gcc      288
Phe Gln Leu Gly His Pro Leu Ala Phe Glu Ala Ala Thr Ala Val Ala         
                85                  90                  95              

ggc ctg atg ccg cag ggc ctg gac cgc gtg ttc ttc acc aat tcg ggc      336
Gly Leu Met Pro Gln Gly Leu Asp Arg Val Phe Phe Thr Asn Ser Gly         
            100                 105                 110                 

tcc gaa tcg gtg gac acc gcg ctg aag atc gcc ctg gcc tac cac cgc      384
Ser Glu Ser Val Asp Thr Ala Leu Lys Ile Ala Leu Ala Tyr His Arg         
        115                 120                 125                     

gcg cgc ggc gag gcg cag cgc acc cgc ctc atc ggg cgc gag cgc ggc      432
Ala Arg Gly Glu Ala Gln Arg Thr Arg Leu Ile Gly Arg Glu Arg Gly         
    130                 135                 140                         

tac cac ggc gtg ggc ttc ggc ggc att tcc gtg ggc ggc atc tcg ccc      480
Tyr His Gly Val Gly Phe Gly Gly Ile Ser Val Gly Gly Ile Ser Pro         
145                 150                 155                 160         

aac cgc aag acc ttc tcc ggc gcg ctg ctg ccg gcc gtg gac cac ctg      528
Asn Arg Lys Thr Phe Ser Gly Ala Leu Leu Pro Ala Val Asp His Leu         
                165                 170                 175             

ccg cac acc cac agc ctg gaa cac aac gcc ttc acg cgc ggc cag ccc      576
Pro His Thr His Ser Leu Glu His Asn Ala Phe Thr Arg Gly Gln Pro         
            180                 185                 190                 

gag tgg ggc gcg cac ctg gcc gac gag ttg gaa cgc atc atc gcc ctg      624
Glu Trp Gly Ala His Leu Ala Asp Glu Leu Glu Arg Ile Ile Ala Leu         
        195                 200                 205                     

cac gac gcc tcc acc atc gcg gcc gtg atc gtc gag ccc atg gcc ggc      672
His Asp Ala Ser Thr Ile Ala Ala Val Ile Val Glu Pro Met Ala Gly         
    210                 215                 220                         

tcc acc ggc gtg ctc gtc ccg ccc aag ggc tat ctc gaa aaa ctg cgc      720
Ser Thr Gly Val Leu Val Pro Pro Lys Gly Tyr Leu Glu Lys Leu Arg         
225                 230                 235                 240         

gaa atc acc gcc cgc cac ggc att ctg ctg atc ttc gac gaa gtc atc      768
Glu Ile Thr Ala Arg His Gly Ile Leu Leu Ile Phe Asp Glu Val Ile         
                245                 250                 255             

acc gcg tac ggc cgc ctg ggc gag gcc acc gcc gcg gcc tat ttc ggc      816
Thr Ala Tyr Gly Arg Leu Gly Glu Ala Thr Ala Ala Ala Tyr Phe Gly         
            260                 265                 270                 

gta acg ccc gac ctc atc acc atg gcc aag ggc gtg agc aac gcc gcc      864
Val Thr Pro Asp Leu Ile Thr Met Ala Lys Gly Val Ser Asn Ala Ala         
        275                 280                 285                     

gtt ccg gcc ggc gcc gtc gcg gtg cgc cgc gaa gtg cat gac gcc atc      912
Val Pro Ala Gly Ala Val Ala Val Arg Arg Glu Val His Asp Ala Ile         
    290                 295                 300                         

gtc aac gga ccg caa ggc ggc atc gag ttc ttc cac ggc tac acc tac      960
Val Asn Gly Pro Gln Gly Gly Ile Glu Phe Phe His Gly Tyr Thr Tyr         
305                 310                 315                 320         

tcg gcc cac ccg ctg gcc gcc gcc gcc gtg ctc gcc acg ctg gac atc     1008
Ser Ala His Pro Leu Ala Ala Ala Ala Val Leu Ala Thr Leu Asp Ile         
                325                 330                 335             

tac cgc cgc gaa gac ctg ttc gcc cgc gcc cgc aag ctg tcg gcc gcg     1056
Tyr Arg Arg Glu Asp Leu Phe Ala Arg Ala Arg Lys Leu Ser Ala Ala         
            340                 345                 350                 

ttc gag gaa gcc gcc cac agc ctc aag ggc gcg ccg cac gtc atc gac     1104
Phe Glu Glu Ala Ala His Ser Leu Lys Gly Ala Pro His Val Ile Asp         
        355                 360                 365                     

gtg cgc aac atc ggc ctg gtg gcc ggc atc gag ctg tcg ccg cgc gaa     1152
Val Arg Asn Ile Gly Leu Val Ala Gly Ile Glu Leu Ser Pro Arg Glu         
    370                 375                 380                         

ggc gcc ccg ggc gcg cgc gcc gcc gaa gcc ttc cag aaa tgc ttc gac     1200
Gly Ala Pro Gly Ala Arg Ala Ala Glu Ala Phe Gln Lys Cys Phe Asp         
385                 390                 395                 400         

acc ggc ctc atg gtg cgc tac acg ggc gac atc ctc gcg gtg tcg cct     1248
Thr Gly Leu Met Val Arg Tyr Thr Gly Asp Ile Leu Ala Val Ser Pro         
                405                 410                 415             

ccg ctc atc gtc gac gaa aac cag atc ggc cag atc ttc gag ggc atc     1296
Pro Leu Ile Val Asp Glu Asn Gln Ile Gly Gln Ile Phe Glu Gly Ile         
            420                 425                 430                 

ggc aag gtg ctc aag gaa gtg gct tag                                 1323
Gly Lys Val Leu Lys Glu Val Ala                                         
        435                 440                                         


<210>  61
<211>  440
<212>  PRT
<213>  Achromobacter denitrificans

<400>  61

Met Ser Ala Ala Lys Leu Pro Asp Leu Ser His Leu Trp Met Pro Phe 
1               5                   10                  15      


Thr Ala Asn Arg Gln Phe Lys Ala Asn Pro Arg Leu Leu Ala Ser Ala 
            20                  25                  30          


Lys Gly Met Tyr Tyr Thr Ser Phe Asp Gly Arg Gln Ile Leu Asp Gly 
        35                  40                  45              


Thr Ala Gly Leu Trp Cys Val Asn Ala Gly His Cys Arg Glu Glu Ile 
    50                  55                  60                  


Val Ser Ala Ile Ala Ser Gln Ala Gly Val Met Asp Tyr Ala Pro Gly 
65                  70                  75                  80  


Phe Gln Leu Gly His Pro Leu Ala Phe Glu Ala Ala Thr Ala Val Ala 
                85                  90                  95      


Gly Leu Met Pro Gln Gly Leu Asp Arg Val Phe Phe Thr Asn Ser Gly 
            100                 105                 110         


Ser Glu Ser Val Asp Thr Ala Leu Lys Ile Ala Leu Ala Tyr His Arg 
        115                 120                 125             


Ala Arg Gly Glu Ala Gln Arg Thr Arg Leu Ile Gly Arg Glu Arg Gly 
    130                 135                 140                 


Tyr His Gly Val Gly Phe Gly Gly Ile Ser Val Gly Gly Ile Ser Pro 
145                 150                 155                 160 


Asn Arg Lys Thr Phe Ser Gly Ala Leu Leu Pro Ala Val Asp His Leu 
                165                 170                 175     


Pro His Thr His Ser Leu Glu His Asn Ala Phe Thr Arg Gly Gln Pro 
            180                 185                 190         


Glu Trp Gly Ala His Leu Ala Asp Glu Leu Glu Arg Ile Ile Ala Leu 
        195                 200                 205             


His Asp Ala Ser Thr Ile Ala Ala Val Ile Val Glu Pro Met Ala Gly 
    210                 215                 220                 


Ser Thr Gly Val Leu Val Pro Pro Lys Gly Tyr Leu Glu Lys Leu Arg 
225                 230                 235                 240 


Glu Ile Thr Ala Arg His Gly Ile Leu Leu Ile Phe Asp Glu Val Ile 
                245                 250                 255     


Thr Ala Tyr Gly Arg Leu Gly Glu Ala Thr Ala Ala Ala Tyr Phe Gly 
            260                 265                 270         


Val Thr Pro Asp Leu Ile Thr Met Ala Lys Gly Val Ser Asn Ala Ala 
        275                 280                 285             


Val Pro Ala Gly Ala Val Ala Val Arg Arg Glu Val His Asp Ala Ile 
    290                 295                 300                 


Val Asn Gly Pro Gln Gly Gly Ile Glu Phe Phe His Gly Tyr Thr Tyr 
305                 310                 315                 320 


Ser Ala His Pro Leu Ala Ala Ala Ala Val Leu Ala Thr Leu Asp Ile 
                325                 330                 335     


Tyr Arg Arg Glu Asp Leu Phe Ala Arg Ala Arg Lys Leu Ser Ala Ala 
            340                 345                 350         


Phe Glu Glu Ala Ala His Ser Leu Lys Gly Ala Pro His Val Ile Asp 
        355                 360                 365             


Val Arg Asn Ile Gly Leu Val Ala Gly Ile Glu Leu Ser Pro Arg Glu 
    370                 375                 380                 


Gly Ala Pro Gly Ala Arg Ala Ala Glu Ala Phe Gln Lys Cys Phe Asp 
385                 390                 395                 400 


Thr Gly Leu Met Val Arg Tyr Thr Gly Asp Ile Leu Ala Val Ser Pro 
                405                 410                 415     


Pro Leu Ile Val Asp Glu Asn Gln Ile Gly Gln Ile Phe Glu Gly Ile 
            420                 425                 430         


Gly Lys Val Leu Lys Glu Val Ala 
        435                 440 


<210>  62
<211>  439
<212>  PRT
<213>  Homo sapiens

<400>  62

Met Phe Ser Lys Leu Ala His Leu Gln Arg Phe Ala Val Leu Ser Arg 
1               5                   10                  15      


Gly Val His Ser Ser Val Ala Ser Ala Thr Ser Val Ala Thr Lys Lys 
            20                  25                  30          


Thr Val Gln Gly Pro Pro Thr Ser Asp Asp Ile Phe Glu Arg Glu Tyr 
        35                  40                  45              


Lys Tyr Gly Ala His Asn Tyr His Pro Leu Pro Val Ala Leu Glu Arg 
    50                  55                  60                  


Gly Lys Gly Ile Tyr Leu Trp Asp Val Glu Gly Arg Lys Tyr Phe Asp 
65                  70                  75                  80  


Phe Leu Ser Ser Tyr Ser Ala Val Asn Gln Gly His Cys His Pro Lys 
                85                  90                  95      


Ile Val Asn Ala Leu Lys Ser Gln Val Asp Lys Leu Thr Leu Thr Ser 
            100                 105                 110         


Arg Ala Phe Tyr Asn Asn Val Leu Gly Glu Tyr Glu Glu Tyr Ile Thr 
        115                 120                 125             


Lys Leu Phe Asn Tyr His Lys Val Leu Pro Met Asn Thr Gly Val Glu 
    130                 135                 140                 


Ala Gly Glu Thr Ala Cys Lys Leu Ala Arg Lys Trp Gly Tyr Thr Val 
145                 150                 155                 160 


Lys Gly Ile Gln Lys Tyr Lys Ala Lys Ile Val Phe Ala Ala Gly Asn 
                165                 170                 175     


Phe Trp Gly Arg Thr Leu Ser Ala Ile Ser Ser Ser Thr Asp Pro Thr 
            180                 185                 190         


Ser Tyr Asp Gly Phe Gly Pro Phe Met Pro Gly Phe Asp Ile Ile Pro 
        195                 200                 205             


Tyr Asn Asp Leu Pro Ala Leu Glu Arg Ala Leu Gln Asp Pro Asn Val 
    210                 215                 220                 


Ala Ala Phe Met Val Glu Pro Ile Gln Gly Glu Ala Gly Val Val Val 
225                 230                 235                 240 


Pro Asp Pro Gly Tyr Leu Met Gly Val Arg Glu Leu Cys Thr Arg His 
                245                 250                 255     


Gln Val Leu Phe Ile Ala Asp Glu Ile Gln Thr Gly Leu Ala Arg Thr 
            260                 265                 270         


Gly Arg Trp Leu Ala Val Asp Tyr Glu Asn Val Arg Pro Asp Ile Val 
        275                 280                 285             


Leu Leu Gly Lys Ala Leu Ser Gly Gly Leu Tyr Pro Val Ser Ala Val 
    290                 295                 300                 


Leu Cys Asp Asp Asp Ile Met Leu Thr Ile Lys Pro Gly Glu His Gly 
305                 310                 315                 320 


Ser Thr Tyr Gly Gly Asn Pro Leu Gly Cys Arg Val Ala Ile Ala Ala 
                325                 330                 335     


Leu Glu Val Leu Glu Glu Glu Asn Leu Ala Glu Asn Ala Asp Lys Leu 
            340                 345                 350         


Gly Ile Ile Leu Arg Asn Glu Leu Met Lys Leu Pro Ser Asp Val Val 
        355                 360                 365             


Thr Ala Val Arg Gly Lys Gly Leu Leu Asn Ala Ile Val Ile Lys Glu 
    370                 375                 380                 


Thr Lys Asp Trp Asp Ala Trp Lys Val Cys Leu Arg Leu Arg Asp Asn 
385                 390                 395                 400 


Gly Leu Leu Ala Lys Pro Thr His Gly Asp Ile Ile Arg Phe Ala Pro 
                405                 410                 415     


Pro Leu Val Ile Lys Glu Asp Glu Leu Arg Glu Ser Ile Glu Ile Ile 
            420                 425                 430         


Asn Lys Thr Ile Leu Ser Phe 
        435                 


<210>  63
<211>  301
<212>  PRT
<213>  Homo sapiens

<400>  63

Met Asn Thr Gly Val Glu Ala Gly Glu Thr Ala Cys Lys Leu Ala Arg 
1               5                   10                  15      


Lys Trp Gly Tyr Thr Val Lys Gly Ile Gln Lys Tyr Lys Ala Lys Ile 
            20                  25                  30          


Val Phe Ala Ala Gly Asn Phe Trp Gly Arg Thr Leu Ser Ala Ile Ser 
        35                  40                  45              


Ser Ser Thr Asp Pro Thr Ser Tyr Asp Gly Phe Gly Pro Phe Met Pro 
    50                  55                  60                  


Gly Phe Asp Ile Ile Pro Tyr Asn Asp Leu Pro Ala Leu Glu Arg Ala 
65                  70                  75                  80  


Leu Gln Asp Pro Asn Val Ala Ala Phe Met Val Glu Pro Ile Gln Gly 
                85                  90                  95      


Glu Ala Gly Val Val Val Pro Asp Pro Gly Tyr Leu Met Gly Val Arg 
            100                 105                 110         


Glu Leu Cys Thr Arg His Gln Val Leu Phe Ile Ala Asp Glu Ile Gln 
        115                 120                 125             


Thr Gly Leu Ala Arg Thr Gly Arg Trp Leu Ala Val Asp Tyr Glu Asn 
    130                 135                 140                 


Val Arg Pro Asp Ile Val Leu Leu Gly Lys Ala Leu Ser Gly Gly Leu 
145                 150                 155                 160 


Tyr Pro Val Ser Ala Val Leu Cys Asp Asp Asp Ile Met Leu Thr Ile 
                165                 170                 175     


Lys Pro Gly Glu His Gly Ser Thr Tyr Gly Gly Asn Pro Leu Gly Cys 
            180                 185                 190         


Arg Val Ala Ile Ala Ala Leu Glu Val Leu Glu Glu Glu Asn Leu Ala 
        195                 200                 205             


Glu Asn Ala Asp Lys Leu Gly Ile Ile Leu Arg Asn Glu Leu Met Lys 
    210                 215                 220                 


Leu Pro Ser Asp Val Val Thr Ala Val Arg Gly Lys Gly Leu Leu Asn 
225                 230                 235                 240 


Ala Ile Val Ile Lys Glu Thr Lys Asp Trp Asp Ala Trp Lys Val Cys 
                245                 250                 255     


Leu Arg Leu Arg Asp Asn Gly Leu Leu Ala Lys Pro Thr His Gly Asp 
            260                 265                 270         


Ile Ile Arg Phe Ala Pro Pro Leu Val Ile Lys Glu Asp Glu Leu Arg 
        275                 280                 285             


Glu Ser Ile Glu Ile Ile Asn Lys Thr Ile Leu Ser Phe 
    290                 295                 300     


<210>  64
<211>  414
<212>  PRT
<213>  Plasmodium yoelii

<400>  64

Met Glu Phe Val Lys Asp Leu Lys Thr Pro Glu Asp Tyr Ile Asn Asn 
1               5                   10                  15      


Glu Leu Lys Tyr Gly Ala His Asn Tyr Asp Pro Ile Pro Val Val Leu 
            20                  25                  30          


Lys Arg Ala Lys Gly Val Phe Val Tyr Asp Val Asn Asp Lys Arg Tyr 
        35                  40                  45              


Tyr Asp Phe Leu Ser Ala Tyr Ser Ser Val Asn Gln Gly His Cys His 
    50                  55                  60                  


Pro Asn Ile Leu Asn Ala Met Ile Asn Gln Ala Lys Asn Leu Thr Ile 
65                  70                  75                  80  


Cys Ser Arg Ala Phe Phe Ser Val Pro Leu Gly Ile Cys Glu Arg Tyr 
                85                  90                  95      


Leu Thr Asn Leu Leu Gly Tyr Asp Lys Val Leu Met Met Asn Thr Gly 
            100                 105                 110         


Ala Glu Ala Asn Glu Thr Ala Tyr Lys Leu Cys Arg Lys Trp Gly Tyr 
        115                 120                 125             


Glu Val Lys Lys Ile Pro Glu Asn Met Ala Lys Ile Val Val Cys Lys 
    130                 135                 140                 


Asn Asn Phe Ser Gly Arg Thr Leu Gly Cys Ile Ser Ala Ser Thr Thr 
145                 150                 155                 160 


Lys Lys Cys Thr Ser Asn Phe Gly Pro Phe Ala Pro Gln Phe Ser Lys 
                165                 170                 175     


Val Pro Tyr Asp Asp Leu Glu Ala Leu Glu Glu Glu Leu Lys Asp Pro 
            180                 185                 190         


Asn Val Cys Ala Phe Ile Val Glu Pro Ile Gln Gly Glu Ala Gly Val 
        195                 200                 205             


Ile Val Pro Ser Asp Asn Tyr Leu Gln Gly Val Tyr Asp Ile Cys Lys 
    210                 215                 220                 


Lys Tyr Asn Val Leu Phe Val Ala Asp Glu Val Gln Thr Gly Leu Gly 
225                 230                 235                 240 


Arg Thr Gly Lys Leu Leu Cys Val His His Tyr Asn Val Lys Pro Asp 
                245                 250                 255     


Val Ile Leu Leu Gly Lys Ala Leu Ser Gly Gly His Tyr Pro Ile Ser 
            260                 265                 270         


Ala Val Leu Ala Asn Asp Asp Ile Met Leu Val Ile Lys Pro Gly Glu 
        275                 280                 285             


His Gly Ser Thr Tyr Gly Gly Asn Pro Leu Ala Ala Ser Ile Cys Val 
    290                 295                 300                 


Glu Ala Leu Asn Val Leu Ile Asn Glu Lys Leu Cys Glu Asn Ala Glu 
305                 310                 315                 320 


Lys Leu Gly Gly Pro Phe Leu Glu Asn Leu Lys Arg Glu Leu Lys Asp 
                325                 330                 335     


Ser Lys Ile Val Arg Asp Val Arg Gly Lys Gly Leu Leu Cys Ala Ile 
            340                 345                 350         


Glu Phe Lys Asn Glu Leu Val Asn Val Leu Asp Ile Cys Leu Lys Leu 
        355                 360                 365             


Lys Glu Asn Gly Leu Ile Thr Arg Asp Val His Asp Lys Thr Ile Arg 
    370                 375                 380                 


Leu Thr Pro Pro Leu Cys Ile Thr Lys Glu Gln Leu Asp Glu Cys Thr 
385                 390                 395                 400 


Glu Ile Ile Val Lys Thr Val Lys Phe Phe Asp Glu Arg Phe 
                405                 410                 


<210>  65
<211>  414
<212>  PRT
<213>  Plasmodium falciparum

<400>  65

Met Asp Phe Val Lys Glu Leu Lys Ser Ser Gln Asp Tyr Met Asn Asn 
1               5                   10                  15      


Glu Leu Thr Tyr Gly Ala His Asn Tyr Asp Pro Ile Pro Val Val Leu 
            20                  25                  30          


Lys Arg Gly Lys Gly Val Phe Val Tyr Asp Ile Glu Asp Arg Arg Tyr 
        35                  40                  45              


Tyr Asp Phe Leu Ser Ala Tyr Ser Ser Val Asn Gln Gly His Cys His 
    50                  55                  60                  


Pro Asp Ile Leu Asn Ala Met Ile Asn Gln Ala Lys Lys Leu Thr Ile 
65                  70                  75                  80  


Cys Ser Arg Ala Phe Phe Ser Asp Ser Leu Gly Val Cys Glu Arg Tyr 
                85                  90                  95      


Leu Thr Asn Leu Phe Gly Tyr Asp Lys Val Leu Met Met Asn Thr Gly 
            100                 105                 110         


Ala Glu Ala Ser Glu Thr Ala Tyr Lys Leu Cys Arg Lys Trp Gly Tyr 
        115                 120                 125             


Glu Val Lys Lys Ile Pro Glu Asn Ser Ala Lys Ile Ile Val Cys Asn 
    130                 135                 140                 


Asn Asn Phe Ser Gly Arg Thr Leu Gly Cys Val Ser Ala Ser Thr Asp 
145                 150                 155                 160 


Lys Lys Cys Lys Asn Asn Phe Gly Pro Phe Val Pro Asn Phe Leu Lys 
                165                 170                 175     


Val Pro Tyr Asp Asp Leu Glu Ala Leu Glu Lys Glu Leu Gln Asp Pro 
            180                 185                 190         


Asn Val Cys Ala Phe Ile Val Glu Pro Val Gln Gly Glu Ala Gly Val 
        195                 200                 205             


Ile Val Pro Ser Asp Ser Tyr Phe Pro Gly Val Ala Ser Leu Cys Lys 
    210                 215                 220                 


Lys Tyr Asn Val Leu Phe Val Ala Asp Glu Val Gln Thr Gly Leu Gly 
225                 230                 235                 240 


Arg Thr Gly Lys Leu Leu Cys Thr His His Tyr Gly Val Lys Pro Asp 
                245                 250                 255     


Val Ile Leu Leu Gly Lys Ala Leu Ser Gly Gly His Tyr Pro Ile Ser 
            260                 265                 270         


Ala Ile Leu Ala Asn Asp Asp Val Met Leu Val Leu Lys Pro Gly Glu 
        275                 280                 285             


His Gly Ser Thr Tyr Gly Gly Asn Pro Leu Ala Ala Ala Ile Cys Val 
    290                 295                 300                 


Glu Ala Leu Lys Val Leu Ile Asn Glu Lys Leu Cys Glu Asn Ala Asp 
305                 310                 315                 320 


Lys Leu Gly Ala Pro Phe Leu Gln Asn Leu Lys Glu Gln Leu Lys Asp 
                325                 330                 335     


Ser Lys Val Val Arg Glu Val Arg Gly Lys Gly Leu Leu Cys Ala Ile 
            340                 345                 350         


Glu Phe Lys Asn Asp Leu Val Asn Val Trp Asp Ile Cys Leu Lys Phe 
        355                 360                 365             


Lys Glu Asn Gly Leu Ile Thr Arg Ser Val His Asp Lys Thr Val Arg 
    370                 375                 380                 


Leu Thr Pro Pro Leu Cys Ile Thr Lys Glu Gln Leu Asp Glu Cys Thr 
385                 390                 395                 400 


Glu Ile Ile Val Lys Thr Val Lys Phe Phe Asp Asp Asn Leu 
                405                 410                 


<210>  66
<211>  396
<212>  PRT
<213>  Bacillus anthracis

<400>  66

Met Ile Gln Thr Lys Asp Ile Ile Glu Leu Thr Asp Thr Tyr Gly Ala 
1               5                   10                  15      


Asn Asn Tyr His Pro Leu Pro Ile Val Ile Ser Lys Ala Glu Gly Val 
            20                  25                  30          


Trp Val Glu Asp Pro Glu Gly Asn Arg Tyr Met Asp Leu Leu Ser Ala 
        35                  40                  45              


Tyr Ser Ala Val Asn Gln Gly His Arg His Pro Lys Ile Ile Asn Ala 
    50                  55                  60                  


Leu Ile Asp Gln Ala Asn Arg Val Thr Leu Thr Ser Arg Ala Phe His 
65                  70                  75                  80  


Ser Asp Gln Leu Gly Pro Trp Tyr Glu Lys Val Ala Lys Leu Thr Asn 
                85                  90                  95      


Lys Glu Met Val Leu Pro Met Asn Thr Gly Ala Glu Ala Val Glu Thr 
            100                 105                 110         


Ala Ile Lys Thr Ala Arg Arg Trp Ala Tyr Asp Val Lys Lys Val Glu 
        115                 120                 125             


Ala Asn Arg Ala Glu Ile Ile Val Cys Glu Asp Asn Phe His Gly Arg 
    130                 135                 140                 


Thr Met Gly Ala Val Ser Met Ser Ser Asn Glu Glu Tyr Lys Arg Gly 
145                 150                 155                 160 


Phe Gly Pro Met Leu Pro Gly Ile Ile Val Ile Pro Tyr Gly Asp Leu 
                165                 170                 175     


Glu Ala Leu Lys Ala Ala Ile Thr Pro Asn Thr Ala Ala Phe Ile Leu 
            180                 185                 190         


Glu Pro Ile Gln Gly Glu Ala Gly Ile Asn Ile Pro Pro Ala Gly Phe 
        195                 200                 205             


Leu Lys Glu Ala Leu Glu Val Cys Lys Lys Glu Asn Val Leu Phe Val 
    210                 215                 220                 


Ala Asp Glu Ile Gln Thr Gly Leu Gly Arg Thr Gly Lys Val Phe Ala 
225                 230                 235                 240 


Cys Asp Trp Asp Asn Val Thr Pro Asp Met Tyr Ile Leu Gly Lys Ala 
                245                 250                 255     


Leu Gly Gly Gly Val Phe Pro Ile Ser Cys Ala Ala Ala Asn Arg Asp 
            260                 265                 270         


Ile Leu Gly Val Phe Glu Pro Gly Ser His Gly Ser Thr Phe Gly Gly 
        275                 280                 285             


Asn Pro Leu Ala Cys Ala Val Ser Ile Ala Ala Leu Glu Val Leu Glu 
    290                 295                 300                 


Glu Glu Lys Leu Thr Glu Arg Ser Leu Gln Leu Gly Glu Lys Leu Val 
305                 310                 315                 320 


Gly Gln Leu Lys Glu Ile Asp Asn Pro Met Ile Thr Glu Val Arg Gly 
                325                 330                 335     


Lys Gly Leu Phe Ile Gly Ile Glu Leu Asn Glu Pro Ala Arg Pro Tyr 
            340                 345                 350         


Cys Glu Gln Leu Lys Ala Ala Gly Leu Leu Cys Lys Glu Thr His Glu 
        355                 360                 365             


Asn Val Ile Arg Ile Ala Pro Pro Leu Val Ile Ser Glu Glu Asp Leu 
    370                 375                 380                 


Glu Trp Ala Phe Gln Lys Ile Lys Ala Val Leu Ser 
385                 390                 395     


<210>  67
<211>  1374
<212>  DNA
<213>  Streptomyces clavuligerus


<220>
<221>  CDS
<222>  (1)..(1374)

<400>  67
atg ggc gaa gca gca cgc cac ccc gac ggc gat ttc tcg gac gtg gga       48
Met Gly Glu Ala Ala Arg His Pro Asp Gly Asp Phe Ser Asp Val Gly         
1               5                   10                  15              

aac ctc cac gct cag gac gtg cac cag gca ctt gag cag cat atg ctc       96
Asn Leu His Ala Gln Asp Val His Gln Ala Leu Glu Gln His Met Leu         
            20                  25                  30                  

gtc gac ggg tac gac ctc gtt ctc gac ctc gac gcc agc tcc ggc gtc      144
Val Asp Gly Tyr Asp Leu Val Leu Asp Leu Asp Ala Ser Ser Gly Val         
        35                  40                  45                      

tgg ctc gtc gac gcc gtc acc cag aag cgg tat ctc gac ctc ttt tcc      192
Trp Leu Val Asp Ala Val Thr Gln Lys Arg Tyr Leu Asp Leu Phe Ser         
    50                  55                  60                          

ttc ttt gcc tcg gcg ccg ctc gga atc aac ccg ccc agc att gtc gag      240
Phe Phe Ala Ser Ala Pro Leu Gly Ile Asn Pro Pro Ser Ile Val Glu         
65                  70                  75                  80          

gac ccg gca ttc atg cgg gag ctg gcc gtg gcc gcg gtc aac aag ccg      288
Asp Pro Ala Phe Met Arg Glu Leu Ala Val Ala Ala Val Asn Lys Pro         
                85                  90                  95              

tcg aac ccc gat ctt tat tcg gtg ccg tac gcc cgt ttc gtc aag acc      336
Ser Asn Pro Asp Leu Tyr Ser Val Pro Tyr Ala Arg Phe Val Lys Thr         
            100                 105                 110                 

ttc gcc cgg gtc ctc ggc gac ccc cgg ctg ccg cgg ctg ttc ttc gtg      384
Phe Ala Arg Val Leu Gly Asp Pro Arg Leu Pro Arg Leu Phe Phe Val         
        115                 120                 125                     

gac ggc ggg gcg ctg gcc gtg gag aac gcg ctc aag gcg gcc ctc gac      432
Asp Gly Gly Ala Leu Ala Val Glu Asn Ala Leu Lys Ala Ala Leu Asp         
    130                 135                 140                         

tgg aag gcc cag aag ctg ggc ctc gcc gag ccg gac acc gac cgg ctc      480
Trp Lys Ala Gln Lys Leu Gly Leu Ala Glu Pro Asp Thr Asp Arg Leu         
145                 150                 155                 160         

cag gtg ctg cat ctg gag cgc tcg ttc cac ggc cgc agc ggc tac acc      528
Gln Val Leu His Leu Glu Arg Ser Phe His Gly Arg Ser Gly Tyr Thr         
                165                 170                 175             

atg tcg ctg acg aac acc gag ccg tcc aag acc gcc cgc ttc ccc aag      576
Met Ser Leu Thr Asn Thr Glu Pro Ser Lys Thr Ala Arg Phe Pro Lys         
            180                 185                 190                 

ttc ggc tgg cca cgg atc tcg tcc ccc gcc ctc cag cac ccg ccg gcc      624
Phe Gly Trp Pro Arg Ile Ser Ser Pro Ala Leu Gln His Pro Pro Ala         
        195                 200                 205                     

gag cac acc ggc gcc aac cag gag gcc gag cga cgg gcg ctg gag gcc      672
Glu His Thr Gly Ala Asn Gln Glu Ala Glu Arg Arg Ala Leu Glu Ala         
    210                 215                 220                         

gcc cgg gag gcg ttc gca gcg gcg gac ggc atg atc gcc tgc ttc atc      720
Ala Arg Glu Ala Phe Ala Ala Ala Asp Gly Met Ile Ala Cys Phe Ile         
225                 230                 235                 240         

gcg gag ccc atc cag ggc gag ggc ggc gac aac cac ctc agc gcg gag      768
Ala Glu Pro Ile Gln Gly Glu Gly Gly Asp Asn His Leu Ser Ala Glu         
                245                 250                 255             

ttc ctc cag gcc atg cag cgg ctc tgc cac gag aac gac gcc ctg ttc      816
Phe Leu Gln Ala Met Gln Arg Leu Cys His Glu Asn Asp Ala Leu Phe         
            260                 265                 270                 

gtc ctg gac gag gtg cag agc ggc tgc ggc atc acc ggt acc gcc tgg      864
Val Leu Asp Glu Val Gln Ser Gly Cys Gly Ile Thr Gly Thr Ala Trp         
        275                 280                 285                     

gcc tac cag cag ctc ggc ctc cag ccc gac ctg gtg gcc ttc ggc aag      912
Ala Tyr Gln Gln Leu Gly Leu Gln Pro Asp Leu Val Ala Phe Gly Lys         
    290                 295                 300                         

aag acc cag gtc tgc ggg gtg atg ggc ggc ggc cgg atc gac gag gtc      960
Lys Thr Gln Val Cys Gly Val Met Gly Gly Gly Arg Ile Asp Glu Val         
305                 310                 315                 320         

ccc gag aac gtc ttc gcc gtc tcc tcc cgg atc agc tcc acc tgg ggc     1008
Pro Glu Asn Val Phe Ala Val Ser Ser Arg Ile Ser Ser Thr Trp Gly         
                325                 330                 335             

ggc aac ctc gcc gac atg gtc cgc gcc acc cgg ctg ctg gag acg atc     1056
Gly Asn Leu Ala Asp Met Val Arg Ala Thr Arg Leu Leu Glu Thr Ile         
            340                 345                 350                 

gag cgc acc cag gtc ttc gac acc gtc gtc cag cgc ggc aag tac ttc     1104
Glu Arg Thr Gln Val Phe Asp Thr Val Val Gln Arg Gly Lys Tyr Phe         
        355                 360                 365                     

cgg gac ggc ctg gag gac ctg gcc gcc cgc cac ccc tcc gtc gtg acc     1152
Arg Asp Gly Leu Glu Asp Leu Ala Ala Arg His Pro Ser Val Val Thr         
    370                 375                 380                         

aac gcc cgc ggc cgg ggc ctg atg tgc gcg gtc gac ctg ccg gac acc     1200
Asn Ala Arg Gly Arg Gly Leu Met Cys Ala Val Asp Leu Pro Asp Thr         
385                 390                 395                 400         

cgg acc cgc aat gag gtg ctg cgg ctc atg tac acg gag cac cag gtc     1248
Arg Thr Arg Asn Glu Val Leu Arg Leu Met Tyr Thr Glu His Gln Val         
                405                 410                 415             

atc gcc ctg ccc tgc ggc ggg cgc agc ctc cgg ttc cgc ccc gcg ctg     1296
Ile Ala Leu Pro Cys Gly Gly Arg Ser Leu Arg Phe Arg Pro Ala Leu         
            420                 425                 430                 

acg atc gcc gag cac gag atc gac cag gcc ctt cag gcg ctg gcg agc     1344
Thr Ile Ala Glu His Glu Ile Asp Gln Ala Leu Gln Ala Leu Ala Ser         
        435                 440                 445                     

agt gtc acg gcg gtc gcc gag agc gtc tga                             1374
Ser Val Thr Ala Val Ala Glu Ser Val                                     
    450                 455                                             


<210>  68
<211>  457
<212>  PRT
<213>  Streptomyces clavuligerus

<400>  68

Met Gly Glu Ala Ala Arg His Pro Asp Gly Asp Phe Ser Asp Val Gly 
1               5                   10                  15      


Asn Leu His Ala Gln Asp Val His Gln Ala Leu Glu Gln His Met Leu 
            20                  25                  30          


Val Asp Gly Tyr Asp Leu Val Leu Asp Leu Asp Ala Ser Ser Gly Val 
        35                  40                  45              


Trp Leu Val Asp Ala Val Thr Gln Lys Arg Tyr Leu Asp Leu Phe Ser 
    50                  55                  60                  


Phe Phe Ala Ser Ala Pro Leu Gly Ile Asn Pro Pro Ser Ile Val Glu 
65                  70                  75                  80  


Asp Pro Ala Phe Met Arg Glu Leu Ala Val Ala Ala Val Asn Lys Pro 
                85                  90                  95      


Ser Asn Pro Asp Leu Tyr Ser Val Pro Tyr Ala Arg Phe Val Lys Thr 
            100                 105                 110         


Phe Ala Arg Val Leu Gly Asp Pro Arg Leu Pro Arg Leu Phe Phe Val 
        115                 120                 125             


Asp Gly Gly Ala Leu Ala Val Glu Asn Ala Leu Lys Ala Ala Leu Asp 
    130                 135                 140                 


Trp Lys Ala Gln Lys Leu Gly Leu Ala Glu Pro Asp Thr Asp Arg Leu 
145                 150                 155                 160 


Gln Val Leu His Leu Glu Arg Ser Phe His Gly Arg Ser Gly Tyr Thr 
                165                 170                 175     


Met Ser Leu Thr Asn Thr Glu Pro Ser Lys Thr Ala Arg Phe Pro Lys 
            180                 185                 190         


Phe Gly Trp Pro Arg Ile Ser Ser Pro Ala Leu Gln His Pro Pro Ala 
        195                 200                 205             


Glu His Thr Gly Ala Asn Gln Glu Ala Glu Arg Arg Ala Leu Glu Ala 
    210                 215                 220                 


Ala Arg Glu Ala Phe Ala Ala Ala Asp Gly Met Ile Ala Cys Phe Ile 
225                 230                 235                 240 


Ala Glu Pro Ile Gln Gly Glu Gly Gly Asp Asn His Leu Ser Ala Glu 
                245                 250                 255     


Phe Leu Gln Ala Met Gln Arg Leu Cys His Glu Asn Asp Ala Leu Phe 
            260                 265                 270         


Val Leu Asp Glu Val Gln Ser Gly Cys Gly Ile Thr Gly Thr Ala Trp 
        275                 280                 285             


Ala Tyr Gln Gln Leu Gly Leu Gln Pro Asp Leu Val Ala Phe Gly Lys 
    290                 295                 300                 


Lys Thr Gln Val Cys Gly Val Met Gly Gly Gly Arg Ile Asp Glu Val 
305                 310                 315                 320 


Pro Glu Asn Val Phe Ala Val Ser Ser Arg Ile Ser Ser Thr Trp Gly 
                325                 330                 335     


Gly Asn Leu Ala Asp Met Val Arg Ala Thr Arg Leu Leu Glu Thr Ile 
            340                 345                 350         


Glu Arg Thr Gln Val Phe Asp Thr Val Val Gln Arg Gly Lys Tyr Phe 
        355                 360                 365             


Arg Asp Gly Leu Glu Asp Leu Ala Ala Arg His Pro Ser Val Val Thr 
    370                 375                 380                 


Asn Ala Arg Gly Arg Gly Leu Met Cys Ala Val Asp Leu Pro Asp Thr 
385                 390                 395                 400 


Arg Thr Arg Asn Glu Val Leu Arg Leu Met Tyr Thr Glu His Gln Val 
                405                 410                 415     


Ile Ala Leu Pro Cys Gly Gly Arg Ser Leu Arg Phe Arg Pro Ala Leu 
            420                 425                 430         


Thr Ile Ala Glu His Glu Ile Asp Gln Ala Leu Gln Ala Leu Ala Ser 
        435                 440                 445             


Ser Val Thr Ala Val Ala Glu Ser Val 
    450                 455         


<210>  69
<211>  1350
<212>  DNA
<213>  Mycobacterium tuberculosis


<220>
<221>  CDS
<222>  (1)..(1350)

<400>  69
atg gcc gcc gtc gtg aag tcc gtc gct ctt gcc ggt aga cca aca aca       48
Met Ala Ala Val Val Lys Ser Val Ala Leu Ala Gly Arg Pro Thr Thr         
1               5                   10                  15              

cca gac cgg gtt cat gag gtg cta ggg cgc agc atg ctg gtc gac ggt       96
Pro Asp Arg Val His Glu Val Leu Gly Arg Ser Met Leu Val Asp Gly         
            20                  25                  30                  

ctg gac ata gtg ctc gat ctg acc cgt tcg ggc ggt tca tat ctg gtc      144
Leu Asp Ile Val Leu Asp Leu Thr Arg Ser Gly Gly Ser Tyr Leu Val         
        35                  40                  45                      

gac gct ata acg ggt cgg cgc tac ctg gac atg ttc aca ttc gtt gcc      192
Asp Ala Ile Thr Gly Arg Arg Tyr Leu Asp Met Phe Thr Phe Val Ala         
    50                  55                  60                          

tcc tcg gca ctg ggt atg aat ccc ccg gcg ctg gtg gac gac cgg gag      240
Ser Ser Ala Leu Gly Met Asn Pro Pro Ala Leu Val Asp Asp Arg Glu         
65                  70                  75                  80          

ttc cat gcc gaa ctc atg cag gcc gcg ctg aac aag ccc agc aat tcc      288
Phe His Ala Glu Leu Met Gln Ala Ala Leu Asn Lys Pro Ser Asn Ser         
                85                  90                  95              

gac gtg tac tcg gtg gcg atg gcc cgc ttc gtc gag acc ttc gcc cgt      336
Asp Val Tyr Ser Val Ala Met Ala Arg Phe Val Glu Thr Phe Ala Arg         
            100                 105                 110                 

gtc ttg ggc gac ccg gcg ctg ccg cat ctg ttc ttc gtc gaa ggg ggc      384
Val Leu Gly Asp Pro Ala Leu Pro His Leu Phe Phe Val Glu Gly Gly         
        115                 120                 125                     

gcc ctg gcg gtg gag aac gcg ctc aaa gcc gcg ttc gac tgg aag agt      432
Ala Leu Ala Val Glu Asn Ala Leu Lys Ala Ala Phe Asp Trp Lys Ser         
    130                 135                 140                         

cgg cac aac caa gcc cat ggg atc gac ccg gcg ctg ggc act caa gtg      480
Arg His Asn Gln Ala His Gly Ile Asp Pro Ala Leu Gly Thr Gln Val         
145                 150                 155                 160         

ctc cac ctg cgc ggg gca ttt cac ggc cgc agc ggc tat acc ctg tcg      528
Leu His Leu Arg Gly Ala Phe His Gly Arg Ser Gly Tyr Thr Leu Ser         
                165                 170                 175             

ctg acc aac acc aag ccg acc att acc gcc cgg ttc ccg aaa ttc gac      576
Leu Thr Asn Thr Lys Pro Thr Ile Thr Ala Arg Phe Pro Lys Phe Asp         
            180                 185                 190                 

tgg cca cgc atc gat gcg ccg tac atg cgg ccc ggc ctg gat gag ccc      624
Trp Pro Arg Ile Asp Ala Pro Tyr Met Arg Pro Gly Leu Asp Glu Pro         
        195                 200                 205                     

gct atg gcc gcg cta gag gcc gag gcg cta cgc cag gcc cgc gcg gca      672
Ala Met Ala Ala Leu Glu Ala Glu Ala Leu Arg Gln Ala Arg Ala Ala         
    210                 215                 220                         

ttc gag acc cgc ccg cac gac atc gcg tgt ttt gtg gcc gaa ccc atc      720
Phe Glu Thr Arg Pro His Asp Ile Ala Cys Phe Val Ala Glu Pro Ile         
225                 230                 235                 240         

cag ggc gag ggt ggc gac cgc cat ttc cgc ccg gag ttt ttc gct gcg      768
Gln Gly Glu Gly Gly Asp Arg His Phe Arg Pro Glu Phe Phe Ala Ala         
                245                 250                 255             

atg cgc gag ctg tgc gac gag ttt gat gca ctg ctg atc ttc gac gaa      816
Met Arg Glu Leu Cys Asp Glu Phe Asp Ala Leu Leu Ile Phe Asp Glu         
            260                 265                 270                 

gta cag acc ggc tgc ggg ttg acc gga acc gcc tgg gca tac cag cag      864
Val Gln Thr Gly Cys Gly Leu Thr Gly Thr Ala Trp Ala Tyr Gln Gln         
        275                 280                 285                     

ttg gat gtc gca ccc gac atc gtg gcg ttc ggc aag aag acg cag gta      912
Leu Asp Val Ala Pro Asp Ile Val Ala Phe Gly Lys Lys Thr Gln Val         
    290                 295                 300                         

tgc gga gtg atg gcc ggt cgg cgg gtg gac gag gtc gcc gac aat gtg      960
Cys Gly Val Met Ala Gly Arg Arg Val Asp Glu Val Ala Asp Asn Val         
305                 310                 315                 320         

ttc gcg gtc cca tca cgg ctc aac tcg aca tgg ggt ggc aat ctt acc     1008
Phe Ala Val Pro Ser Arg Leu Asn Ser Thr Trp Gly Gly Asn Leu Thr         
                325                 330                 335             

gac atg gtg cgc gcc cgc cgc atc ttg gag gtc atc gaa gcc gag ggc     1056
Asp Met Val Arg Ala Arg Arg Ile Leu Glu Val Ile Glu Ala Glu Gly         
            340                 345                 350                 

ctg ttc gag cgg gcg gtg cag cac ggt aag tat ctg cgc gcc cgg ctt     1104
Leu Phe Glu Arg Ala Val Gln His Gly Lys Tyr Leu Arg Ala Arg Leu         
        355                 360                 365                     

gac gaa ctc gcc gcg gac ttc ccg gca gtg gtt ctc gat ccg cgc ggc     1152
Asp Glu Leu Ala Ala Asp Phe Pro Ala Val Val Leu Asp Pro Arg Gly         
    370                 375                 380                         

cgc ggg ctg atg tgt gcg ttt agc ctg ccg acc acc gcc gac cgt gac     1200
Arg Gly Leu Met Cys Ala Phe Ser Leu Pro Thr Thr Ala Asp Arg Asp         
385                 390                 395                 400         

gag ttg atc cgc cag ctg tgg caa cgt gcg gtg att gtg ttg ccg gcc     1248
Glu Leu Ile Arg Gln Leu Trp Gln Arg Ala Val Ile Val Leu Pro Ala         
                405                 410                 415             

ggt gca gac acc gtg cga ttc cgt cca ccg ctg acg gtt tca acc gcc     1296
Gly Ala Asp Thr Val Arg Phe Arg Pro Pro Leu Thr Val Ser Thr Ala         
            420                 425                 430                 

gag atc gac gcc gcg ata gcc gcg gtg cgc agc gcg tta ccg gtg gtg     1344
Glu Ile Asp Ala Ala Ile Ala Ala Val Arg Ser Ala Leu Pro Val Val         
        435                 440                 445                     

acg taa                                                             1350
Thr                                                                     
                                                                        


<210>  70
<211>  449
<212>  PRT
<213>  Mycobacterium tuberculosis

<400>  70

Met Ala Ala Val Val Lys Ser Val Ala Leu Ala Gly Arg Pro Thr Thr 
1               5                   10                  15      


Pro Asp Arg Val His Glu Val Leu Gly Arg Ser Met Leu Val Asp Gly 
            20                  25                  30          


Leu Asp Ile Val Leu Asp Leu Thr Arg Ser Gly Gly Ser Tyr Leu Val 
        35                  40                  45              


Asp Ala Ile Thr Gly Arg Arg Tyr Leu Asp Met Phe Thr Phe Val Ala 
    50                  55                  60                  


Ser Ser Ala Leu Gly Met Asn Pro Pro Ala Leu Val Asp Asp Arg Glu 
65                  70                  75                  80  


Phe His Ala Glu Leu Met Gln Ala Ala Leu Asn Lys Pro Ser Asn Ser 
                85                  90                  95      


Asp Val Tyr Ser Val Ala Met Ala Arg Phe Val Glu Thr Phe Ala Arg 
            100                 105                 110         


Val Leu Gly Asp Pro Ala Leu Pro His Leu Phe Phe Val Glu Gly Gly 
        115                 120                 125             


Ala Leu Ala Val Glu Asn Ala Leu Lys Ala Ala Phe Asp Trp Lys Ser 
    130                 135                 140                 


Arg His Asn Gln Ala His Gly Ile Asp Pro Ala Leu Gly Thr Gln Val 
145                 150                 155                 160 


Leu His Leu Arg Gly Ala Phe His Gly Arg Ser Gly Tyr Thr Leu Ser 
                165                 170                 175     


Leu Thr Asn Thr Lys Pro Thr Ile Thr Ala Arg Phe Pro Lys Phe Asp 
            180                 185                 190         


Trp Pro Arg Ile Asp Ala Pro Tyr Met Arg Pro Gly Leu Asp Glu Pro 
        195                 200                 205             


Ala Met Ala Ala Leu Glu Ala Glu Ala Leu Arg Gln Ala Arg Ala Ala 
    210                 215                 220                 


Phe Glu Thr Arg Pro His Asp Ile Ala Cys Phe Val Ala Glu Pro Ile 
225                 230                 235                 240 


Gln Gly Glu Gly Gly Asp Arg His Phe Arg Pro Glu Phe Phe Ala Ala 
                245                 250                 255     


Met Arg Glu Leu Cys Asp Glu Phe Asp Ala Leu Leu Ile Phe Asp Glu 
            260                 265                 270         


Val Gln Thr Gly Cys Gly Leu Thr Gly Thr Ala Trp Ala Tyr Gln Gln 
        275                 280                 285             


Leu Asp Val Ala Pro Asp Ile Val Ala Phe Gly Lys Lys Thr Gln Val 
    290                 295                 300                 


Cys Gly Val Met Ala Gly Arg Arg Val Asp Glu Val Ala Asp Asn Val 
305                 310                 315                 320 


Phe Ala Val Pro Ser Arg Leu Asn Ser Thr Trp Gly Gly Asn Leu Thr 
                325                 330                 335     


Asp Met Val Arg Ala Arg Arg Ile Leu Glu Val Ile Glu Ala Glu Gly 
            340                 345                 350         


Leu Phe Glu Arg Ala Val Gln His Gly Lys Tyr Leu Arg Ala Arg Leu 
        355                 360                 365             


Asp Glu Leu Ala Ala Asp Phe Pro Ala Val Val Leu Asp Pro Arg Gly 
    370                 375                 380                 


Arg Gly Leu Met Cys Ala Phe Ser Leu Pro Thr Thr Ala Asp Arg Asp 
385                 390                 395                 400 


Glu Leu Ile Arg Gln Leu Trp Gln Arg Ala Val Ile Val Leu Pro Ala 
                405                 410                 415     


Gly Ala Asp Thr Val Arg Phe Arg Pro Pro Leu Thr Val Ser Thr Ala 
            420                 425                 430         


Glu Ile Asp Ala Ala Ile Ala Ala Val Arg Ser Ala Leu Pro Val Val 
        435                 440                 445             


Thr 
    


