                         SEQUENCE LISTING

<110>  NETRIS PHARMA
 
<120>  Combined treatment with netrin-1 interfering drug and 
       chemotherapeutic drug

<130>  BET13L2488

<150>  EP 12306100.4
<151>  2012-09-12

<150>  US 61/700158
<151>  2012-09-12

<160>  14    

<170>  PatentIn version 3.5

<210>  1
<211>  446
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  UNC5A-fusion protein UNC5A-TRAP

<400>  1

Met Asp Phe Gly Leu Arg Leu Ile Phe Leu Val Leu Val Leu Lys Gly 
1               5                   10                  15      


Val Leu Cys Leu Pro His Phe Leu Val Glu Pro Glu Asp Val Tyr Ile 
            20                  25                  30          


Val Lys Asn Lys Pro Val Leu Leu Val Cys Lys Ala Val Pro Ala Thr 
        35                  40                  45              


Gln Ile Phe Phe Lys Cys Asn Gly Glu Trp Val Arg Gln Val Asp His 
    50                  55                  60                  


Val Ile Glu Arg Ser Thr Asp Gly Ser Ser Gly Leu Pro Thr Met Glu 
65                  70                  75                  80  


Val Arg Ile Asn Val Ser Arg Gln Gln Val Glu Lys Val Phe Gly Leu 
                85                  90                  95      


Glu Glu Tyr Trp Cys Gln Cys Val Ala Trp Ser Ser Ser Gly Thr Thr 
            100                 105                 110         


Lys Ser Gln Lys Ala Tyr Ile Arg Ile Ala Tyr Leu Arg Lys Asn Phe 
        115                 120                 125             


Glu Gln Glu Pro Leu Ala Lys Glu Val Ser Leu Glu Gln Gly Ile Val 
    130                 135                 140                 


Leu Pro Cys Arg Pro Pro Glu Gly Ile Pro Pro Ala Glu Val Glu Trp 
145                 150                 155                 160 


Leu Arg Asn Glu Asp Leu Val Asp Pro Ser Leu Asp Pro Asn Val Tyr 
                165                 170                 175     


Ile Thr Arg Glu His Ser Leu Val Val Arg Gln Ala Arg Leu Ala Asp 
            180                 185                 190         


Thr Ala Asn Tyr Thr Cys Val Ala Lys Asn Ile Val Ala Arg Arg Arg 
        195                 200                 205             


Ser Ala Ser Ala Ala Val Ile Val Tyr Gly Thr Asp Lys Thr His Thr 
    210                 215                 220                 


Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe 
225                 230                 235                 240 


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 
                245                 250                 255     


Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 
            260                 265                 270         


Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 
        275                 280                 285             


Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val 
    290                 295                 300                 


Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 
305                 310                 315                 320 


Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser 
                325                 330                 335     


Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
            340                 345                 350         


Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
        355                 360                 365             


Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
    370                 375                 380                 


Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
385                 390                 395                 400 


Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 
                405                 410                 415     


Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 
            420                 425                 430         


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
        435                 440                 445     


<210>  2
<211>  444
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  UNC5B-fusion protein UNC5B-TRAP

<400>  2

Met Asp Phe Gly Leu Arg Leu Ile Phe Leu Val Leu Val Leu Lys Gly 
1               5                   10                  15      


Val Leu Cys Tyr Phe Leu Gln Glu Pro Gln Asp Ala Tyr Ile Val Lys 
            20                  25                  30          


Asn Lys Pro Val Glu Leu Arg Cys Arg Ala Phe Pro Ala Thr Gln Ile 
        35                  40                  45              


Tyr Phe Lys Cys Asn Gly Glu Trp Val Ser Gln Asn Asp His Val Thr 
    50                  55                  60                  


Gln Glu Gly Leu Asp Glu Ala Thr Gly Leu Arg Val Arg Glu Val Gln 
65                  70                  75                  80  


Ile Glu Val Ser Arg Gln Gln Val Glu Glu Leu Phe Gly Leu Glu Asp 
                85                  90                  95      


Tyr Trp Cys Gln Cys Val Ala Trp Ser Ser Ala Gly Thr Thr Lys Ser 
            100                 105                 110         


Arg Arg Ala Tyr Val Arg Ile Ala Tyr Leu Arg Lys Asn Phe Asp Gln 
        115                 120                 125             


Glu Pro Leu Gly Lys Glu Val Pro Leu Asp His Glu Val Leu Leu Gln 
    130                 135                 140                 


Cys Arg Pro Pro Glu Gly Val Pro Val Ala Glu Val Glu Trp Leu Lys 
145                 150                 155                 160 


Asn Glu Asp Val Ile Asp Pro Thr Gln Asp Thr Asn Phe Leu Leu Thr 
                165                 170                 175     


Ile Asp His Asn Leu Ile Ile Arg Gln Ala Arg Leu Ser Asp Thr Ala 
            180                 185                 190         


Asn Tyr Thr Cys Val Ala Lys Asn Ile Val Ala Lys Arg Arg Ser Thr 
        195                 200                 205             


Thr Ala Thr Val Ile Val Tyr Gly Thr Asp Lys Thr His Thr Cys Pro 
    210                 215                 220                 


Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe 
225                 230                 235                 240 


Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val 
                245                 250                 255     


Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe 
            260                 265                 270         


Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro 
        275                 280                 285             


Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr 
    290                 295                 300                 


Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val 
305                 310                 315                 320 


Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala 
                325                 330                 335     


Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg 
            340                 345                 350         


Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly 
        355                 360                 365             


Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro 
    370                 375                 380                 


Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser 
385                 390                 395                 400 


Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln 
                405                 410                 415     


Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His 
            420                 425                 430         


Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
        435                 440                 


<210>  3
<211>  446
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  UNC5C-fusion protein UNC5C-TRAP

<400>  3

Met Asp Phe Gly Leu Arg Leu Ile Phe Leu Val Leu Val Leu Lys Gly 
1               5                   10                  15      


Val Leu Cys Leu Pro His Phe Leu Ile Glu Pro Glu Glu Ala Tyr Ile 
            20                  25                  30          


Val Lys Asn Lys Pro Val Asn Leu Tyr Cys Lys Ala Ser Pro Ala Thr 
        35                  40                  45              


Gln Ile Tyr Phe Lys Cys Asn Ser Glu Trp Val His Gln Lys Asp His 
    50                  55                  60                  


Ile Val Asp Glu Arg Val Asp Glu Thr Ser Gly Leu Ile Val Arg Glu 
65                  70                  75                  80  


Val Ser Ile Glu Ile Ser Arg Gln Gln Val Glu Glu Leu Phe Gly Pro 
                85                  90                  95      


Glu Asp Tyr Trp Cys Gln Cys Val Ala Trp Ser Ser Ala Gly Thr Thr 
            100                 105                 110         


Lys Ser Arg Lys Ala Tyr Val Arg Ile Ala Tyr Leu Arg Lys Thr Phe 
        115                 120                 125             


Glu Gln Glu Pro Leu Gly Lys Glu Val Ser Leu Glu Gln Glu Val Leu 
    130                 135                 140                 


Leu Gln Cys Arg Pro Pro Glu Gly Ile Pro Val Ala Glu Val Glu Trp 
145                 150                 155                 160 


Leu Lys Asn Glu Asp Ile Ile Asp Pro Val Glu Asp Arg Asn Phe Tyr 
                165                 170                 175     


Ile Thr Ile Asp His Asn Leu Ile Ile Lys Gln Ala Arg Leu Ser Asp 
            180                 185                 190         


Thr Ala Asn Tyr Thr Cys Val Ala Lys Asn Ile Val Ala Lys Arg Lys 
        195                 200                 205             


Ser Thr Thr Ala Thr Val Ile Val Tyr Gly Thr Asp Lys Thr His Thr 
    210                 215                 220                 


Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe 
225                 230                 235                 240 


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 
                245                 250                 255     


Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 
            260                 265                 270         


Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 
        275                 280                 285             


Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val 
    290                 295                 300                 


Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 
305                 310                 315                 320 


Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser 
                325                 330                 335     


Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
            340                 345                 350         


Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
        355                 360                 365             


Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
    370                 375                 380                 


Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
385                 390                 395                 400 


Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 
                405                 410                 415     


Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 
            420                 425                 430         


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
        435                 440                 445     


<210>  4
<211>  446
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  UNC5D-fusion protein UNC5D-TRAP

<400>  4

Met Asp Phe Gly Leu Arg Leu Ile Phe Leu Val Leu Val Leu Lys Gly 
1               5                   10                  15      


Val Leu Cys Leu Pro His Phe Ile Glu Glu Pro Asp Asp Ala Tyr Ile 
            20                  25                  30          


Ile Lys Ser Asn Pro Ile Ala Leu Arg Cys Lys Ala Arg Pro Ala Met 
        35                  40                  45              


Gln Ile Phe Phe Lys Cys Asn Gly Glu Trp Val His Gln Asn Glu His 
    50                  55                  60                  


Val Ser Glu Glu Thr Leu Asp Glu Ser Ser Gly Leu Lys Val Arg Glu 
65                  70                  75                  80  


Val Phe Ile Asn Val Thr Arg Gln Gln Val Glu Asp Phe His Gly Pro 
                85                  90                  95      


Glu Asp Tyr Trp Cys Gln Cys Val Ala Trp Ser His Leu Gly Thr Ser 
            100                 105                 110         


Lys Ser Arg Lys Ala Ser Val Arg Ile Ala Tyr Leu Arg Lys Asn Phe 
        115                 120                 125             


Glu Gln Asp Pro Gln Gly Arg Glu Val Pro Ile Glu Gly Met Ile Val 
    130                 135                 140                 


Leu His Cys Arg Pro Pro Glu Gly Val Pro Ala Ala Glu Val Glu Trp 
145                 150                 155                 160 


Leu Lys Asn Glu Glu Pro Ile Asp Ser Glu Gln Asp Glu Asn Ile Asp 
                165                 170                 175     


Thr Arg Ala Asp His Asn Leu Ile Ile Arg Gln Ala Arg Leu Ser Asp 
            180                 185                 190         


Ser Gly Asn Tyr Thr Cys Met Ala Ala Asn Ile Val Ala Lys Arg Arg 
        195                 200                 205             


Ser Leu Ser Ala Thr Val Val Val Tyr Gly Thr Asp Lys Thr His Thr 
    210                 215                 220                 


Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe 
225                 230                 235                 240 


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 
                245                 250                 255     


Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 
            260                 265                 270         


Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 
        275                 280                 285             


Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val 
    290                 295                 300                 


Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 
305                 310                 315                 320 


Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser 
                325                 330                 335     


Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
            340                 345                 350         


Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
        355                 360                 365             


Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
    370                 375                 380                 


Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
385                 390                 395                 400 


Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 
                405                 410                 415     


Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 
            420                 425                 430         


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
        435                 440                 445     


<210>  5
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  UNC5A DNA sequence

<400>  5
aagcttgccg ccaccatgga cttcggcctg cggctgatct tcctggtgct ggtgctgaag       60

ggcgtgctgt gcctgcccca cttcctggtg gaacccgagg acgtgtacat cgtgaagaac      120

aagcccgtgc tgctggtgtg caaggccgtg cccgccaccc agattttctt caagtgcaac      180

ggcgagtggg tgcgccaggt ggaccacgtg atcgagagaa gcaccgacgg cagcagcggc      240

ctgcccacaa tggaagtgcg gatcaacgtg tcccggcagc aggtggaaaa ggtgttcggc      300

ctggaagagt actggtgcca gtgcgtggcc tggtccagca gcggcaccac caagagccag      360

aaggcctaca tccggatcgc ctacctgcgg aagaacttcg agcaggaacc cctggccaaa      420

gaggtgtccc tggaacaggg catcgtgctg ccctgcagac cccctgaggg cattccccct      480

gccgaggtgg aatggctgcg gaacgaggac ctggtggacc ccagcctgga ccccaatgtg      540

tacatcaccc gcgagcacag cctggtggtg agacaggccc gcctggccga caccgccaac      600

tacacctgtg tggccaagaa catcgtggcc agacggcgct ctgcctctgc cgccgtgatc      660

gtgtacggta cc                                                          672


<210>  6
<211>  666
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  UNC5B DNA sequence

<400>  6
aagcttgccg ccaccatgga cttcggcctg cggctgatct tcctggtgct ggtgctgaag       60

ggcgtgctgt gctactttct gcaagaaccc caggacgcct acatcgtgaa gaacaagccc      120

gtggaactgc ggtgccgggc cttccctgcc acccaaatct acttcaagtg caacggcgag      180

tgggtgtccc agaacgacca cgtgacccag gaaggcctgg acgaggccac aggcctgaga      240

gtgcgcgagg tgcagatcga ggtgtcccgg cagcaggtgg aagaactgtt cggcctggaa      300

gattactggt gccagtgcgt ggcctggtct agcgccggca ccaccaagag cagacgggcc      360

tacgtgcgga tcgcctacct gcggaagaac ttcgaccagg aacccctggg caaagaggtg      420

cccctggacc acgaggtgct gctgcagtgc agacctcctg agggcgtgcc cgtggccgag      480

gtggaatggc tgaagaacga ggacgtgatc gaccctaccc aggataccaa cttcctgctg      540

accatcgacc acaacctgat catccggcag gcccggctga gcgacaccgc caattacacc      600

tgtgtggcca agaacatcgt ggccaagcgg cggagcacca ccgccaccgt gatcgtgtac      660

ggtacc                                                                 666


<210>  7
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  UNC5C DNA sequence

<400>  7
aagcttgccg ccaccatgga cttcggcctg cggctgatct tcctggtgct ggtgctgaag       60

ggcgtgctgt gcctgcccca cttcctgatc gagcccgaag aggcctacat cgtgaagaac      120

aagcccgtga acctgtactg caaggccagc cccgccaccc aaatctactt caagtgcaac      180

agcgagtggg tgcaccagaa agaccacatc gtggacgagc gggtggacga gacaagcggc      240

ctgatcgtgc gcgaggtgtc catcgagatc agccggcagc aggtggaaga actgttcggc      300

cccgaggact actggtgcca gtgcgtggcc tggtctagcg ccggcaccac caagagccgg      360

aaggcctacg tgcggatcgc ctacctgaga aagaccttcg agcaggaacc cctgggcaaa      420

gaggtgtccc tggaacaaga agtgctgctg cagtgcagac cccccgaggg aatccccgtg      480

gccgaggtgg aatggctgaa gaacgaggac atcatcgacc ccgtggaaga tcggaacttc      540

tacatcacca tcgaccacaa cctgatcatc aagcaggccc ggctgagcga caccgccaac      600

tacacctgtg tggccaagaa catcgtggcc aagcggaagt ccaccaccgc caccgtgatc      660

gtgtacggta cc                                                          672


<210>  8
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  UNC5D DNA sequence

<400>  8
aagcttgccg ccaccatgga cttcggcctg cggctgatct tcctggtgct ggtgctgaag       60

ggcgtgctgt gcctgcccca cttcatcgaa gagcccgacg acgcctacat catcaagagc      120

aaccccatcg ccctgcgctg caaggcccgc cccgccatgc agattttctt caagtgcaac      180

ggcgagtggg tgcaccagaa cgagcacgtg agcgaggaga ccctggacga gagcagcggc      240

ctgaaggtgc gggaagtgtt catcaacgtg acacggcagc aggtggaaga cttccacggc      300

cccgaagact actggtgcca gtgcgtggcc tggtcccacc tgggcaccag caagagccgg      360

aaggccagcg tgcggatcgc ctacctgcgg aagaacttcg agcaggaccc ccagggccgg      420

gaggtgccca tcgagggcat gatcgtgctg cactgcagac cccctgaggg cgtgcccgcc      480

gccgaggtgg aatggctgaa gaacgaggag cccattgaca gcgagcagga cgagaatatt      540

gacacccgcg ccgaccacaa tctgattatt agacaggccc gcctgagcga cagcggcaac      600

tacacctgta tggccgccaa catcgtggcc aagcggcgct ctctgtctgc caccgtggtg      660

gtgtacggta cc                                                          672


<210>  9
<211>  699
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human IgG1 Fc coding sequence

<400>  9
ggtaccgaca agacccacac ctgtccccca tgccctgccc ctgaactgct gggaggcccc       60

agcgtgttcc tgttcccccc aaagcccaag gacaccctga tgatcagccg gacccccgaa      120

gtgacctgcg tggtggtgga cgtgtcccac gaggaccctg aagtgaagtt caattggtac      180

gtggacggcg tggaagtgca caacgccaag accaagccca gagaggaaca gtacaacagc      240

acctaccggg tggtgtccgt gctgaccgtg ctgcaccagg actggctgaa cggcaaagag      300

tacaagtgca aagtctccaa caaggccctg cctgccccca tcgagaaaac catcagcaag      360

gccaagggac agccccgcga gcctcaggtg tacacactgc cccccagccg ggaagagatg      420

accaagaacc aggtgtccct gacctgcctg gtcaagggct tttaccccag cgatatcgcc      480

gtggaatggg agagcaacgg ccagcccgag aacaattaca agaccacccc ccctgtgctg      540

gacagcgacg gctcattctt cctgtacagc aagctgaccg tggacaagag ccggtggcag      600

cagggcaacg tgttcagctg cagcgtgatg cacgaggccc tgcacaacca ctacacccag      660

aagtccctga gcctgagccc cggcaagtaa taatctaga                             699


<210>  10
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA targeting netrin-1

<400>  10
aagcuggacg cagcaugaug c                                                 21


<210>  11
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer

<400>  11
aaaagtactg caagaaggac tatgc                                             25


<210>  12
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer

<400>  12
ccctgcttat acacggagat g                                                 21


<210>  13
<211>  580
<212>  PRT
<213>  Homo sapiens

<400>  13

Gly Pro Gly Leu Ser Met Phe Ala Gly Gln Ala Ala Gln Pro Asp Pro 
1               5                   10                  15      


Cys Ser Asp Glu Asn Gly His Pro Arg Arg Cys Ile Pro Asp Phe Val 
            20                  25                  30          


Asn Ala Ala Phe Gly Lys Asp Val Arg Val Ser Ser Thr Cys Gly Arg 
        35                  40                  45              


Pro Pro Ala Arg Tyr Cys Val Val Ser Glu Arg Gly Glu Glu Arg Leu 
    50                  55                  60                  


Arg Ser Cys His Leu Cys Asn Ala Ser Asp Pro Lys Lys Ala His Pro 
65                  70                  75                  80  


Pro Ala Phe Leu Thr Asp Leu Asn Asn Pro His Asn Leu Thr Cys Trp 
                85                  90                  95      


Gln Ser Glu Asn Tyr Leu Gln Phe Pro His Asn Val Thr Leu Thr Leu 
            100                 105                 110         


Ser Leu Gly Lys Lys Phe Glu Val Thr Tyr Val Ser Leu Gln Phe Cys 
        115                 120                 125             


Ser Pro Arg Pro Glu Ser Met Ala Ile Tyr Lys Ser Met Asp Tyr Gly 
    130                 135                 140                 


Arg Thr Trp Val Pro Phe Gln Phe Tyr Ser Thr Gln Cys Arg Lys Met 
145                 150                 155                 160 


Tyr Asn Arg Pro His Arg Ala Pro Ile Thr Lys Gln Asn Glu Gln Glu 
                165                 170                 175     


Ala Val Cys Thr Asp Ser His Thr Asp Met Arg Pro Leu Ser Gly Gly 
            180                 185                 190         


Leu Ile Ala Phe Ser Thr Leu Asp Gly Arg Pro Ser Ala His Asp Phe 
        195                 200                 205             


Asp Asn Ser Pro Val Leu Gln Asp Trp Val Thr Ala Thr Asp Ile Arg 
    210                 215                 220                 


Val Ala Phe Ser Arg Leu His Thr Phe Gly Asp Glu Asn Glu Asp Asp 
225                 230                 235                 240 


Ser Glu Leu Ala Arg Asp Ser Tyr Phe Tyr Ala Val Ser Asp Leu Gln 
                245                 250                 255     


Val Gly Gly Arg Cys Lys Cys Asn Gly His Ala Ala Arg Cys Val Arg 
            260                 265                 270         


Asp Arg Thr Asp Ser Leu Val Cys Asp Cys Arg His Asn Thr Ala Gly 
        275                 280                 285             


Pro Glu Cys Asp Arg Cys Lys Pro Phe His Tyr Asp Arg Pro Trp Gln 
    290                 295                 300                 


Arg Ala Thr Ala Arg Glu Ala Asn Glu Cys Val Ala Cys Asn Cys Asn 
305                 310                 315                 320 


Leu His Ala Arg Arg Cys Arg Phe Asn Met Glu Leu Tyr Lys Leu Ser 
                325                 330                 335     


Gly Arg Lys Ser Gly Gly Val Cys Leu Asn Cys Arg His Asn Thr Ala 
            340                 345                 350         


Gly Arg His Cys His Tyr Cys Lys Glu Gly Tyr Tyr Arg Asp Met Gly 
        355                 360                 365             


Lys Pro Ile Thr His Arg Lys Ala Cys Lys Ala Cys Asp Cys His Pro 
    370                 375                 380                 


Val Gly Ala Ala Gly Lys Thr Cys Asn Gln Thr Thr Gly Gln Cys Pro 
385                 390                 395                 400 


Cys Lys Asp Gly Val Thr Gly Ile Thr Cys Asn Arg Cys Ala Lys Gly 
                405                 410                 415     


Tyr Gln Gln Ser Arg Ser Pro Ile Ala Pro Cys Ile Lys Ile Pro Val 
            420                 425                 430         


Ala Pro Pro Thr Thr Ala Ala Ser Ser Val Glu Glu Pro Glu Asp Cys 
        435                 440                 445             


Asp Ser Tyr Cys Lys Ala Ser Lys Gly Lys Leu Lys Ile Asn Met Lys 
    450                 455                 460                 


Lys Tyr Cys Lys Lys Asp Tyr Ala Val Gln Ile His Ile Leu Lys Ala 
465                 470                 475                 480 


Asp Lys Ala Gly Asp Trp Trp Lys Phe Thr Val Asn Ile Ile Ser Val 
                485                 490                 495     


Tyr Lys Gln Gly Thr Ser Arg Ile Arg Arg Gly Asp Gln Ser Leu Trp 
            500                 505                 510         


Ile Arg Ser Arg Asp Ile Ala Cys Lys Cys Pro Lys Ile Lys Pro Leu 
        515                 520                 525             


Lys Lys Tyr Leu Leu Leu Gly Asn Ala Glu Asp Ser Pro Asp Gln Ser 
    530                 535                 540                 


Gly Ile Val Ala Asp Lys Ser Ser Leu Val Ile Gln Trp Arg Asp Thr 
545                 550                 555                 560 


Trp Ala Arg Arg Leu Arg Lys Phe Gln Gln Arg Glu Lys Lys Gly Lys 
                565                 570                 575     


Cys Lys Lys Ala 
            580 


<210>  14
<211>  1815
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  human netrin-1 cDNA sequence

<400>  14
atgatgcgcg cagtgtggga ggcgctggcg gcgctggcgg cggtggcgtg cctggtgggc       60

gcggtgcgcg gcgggcccgg gctcagcatg ttcgcgggcc aggcggcgca gcccgatccc      120

tgctcggacg agaacggcca cccgcgccgc tgcatcccgg actttgtcaa tgcggccttc      180

ggcaaggacg tgcgcgtgtc cagcacctgc ggccggcccc cggcgcgcta ctgcgtggtg      240

agcgagcgcg gcgaggagcg gctgcgctcg tgccacctct gcaacgcgtc cgaccccaag      300

aaggcgcacc cgcccgcctt cctcaccgac ctcaacaacc cgcacaacct gacgtgctgg      360

cagtccgaga actacctgca gttcccgcac aacgtcacgc tcacactgtc cctcggcaag      420

aagttcgaag tgacctacgt gagcctgcag ttctgctcgc cgcggcccga gtccatggcc      480

atctacaagt ccatggacta cgggcgcacg tgggtgccct tccagttcta ctccacgcag      540

tgccgcaaga tgtacaaccg gccgcaccgc gcgcccatca ccaagcagaa cgagcaggag      600

gccgtgtgca ccgactcgca caccgacatg cgcccgctct cgggcggcct catcgccttc      660

agcacgctgg acgggcggcc ctcggcgcac gacttcgaca actcgcccgt gctgcaggac      720

tgggtcacgg ccacagacat ccgcgtggcc ttcagccgcc tgcacacgtt cggcgacgag      780

aacgaggacg actcggagct ggcgcgcgac tcgtacttct acgcggtgtc cgacctgcag      840

gtgggcggcc ggtgcaagtg caacggccac gcggcccgct gcgtgcgcga ccgcaccgac      900

agcctggtgt gcgactgcag gcacaacacg gccggcccgg agtgcgaccg ctgcaagccc      960

ttccactacg accggccctg gcagcgcgcc acagcccgcg aagccaacga gtgcgtggcc     1020

tgtaactgca acctgcatgc ccggcgctgc cgcttcaaca tggagctcta caagctttcg     1080

gggcgcaaga gcggaggtgt ctgcctcaac tgtcgccaca acaccgccgg ccgccactgc     1140

cattactgca aggagggcta ctaccgcgac atgggcaagc ccatcaccca ccggaaggcc     1200

tgcaaagcct gtgattgcca ccctgtgggt gctgctggca aaacctgcaa ccaaaccacc     1260

ggccagtgtc cctgcaagga cggcgtgacg ggtatcacct gcaaccgctg cgccaaaggc     1320

taccagcaga gccgctctcc catcgccccc tgcataaaga tccctgtagc gccgccgacg     1380

actgcagcca gcagcgtgga ggagcctgaa gactgcgatt cctactgcaa ggcctccaag     1440

gggaagctga agattaacat gaaaaagtac tgcaagaagg actatgccgt ccagatccac     1500

atcctgaagg cggacaaggc gggggactgg tggaagttca cggtgaacat catctccgtg     1560

tataagcagg gcacgagccg catccgccgc ggtgaccaga gcctgtggat ccgctcgcgg     1620

gacatcgcct gcaagtgtcc caaaatcaag cccctcaaga agtacctgct gctgggcaac     1680

gcggaggact ctccggacca gagcggcatc gtggccgata aaagcagcct ggtgatccag     1740

tggcgggaca cgtgggcgcg gcggctgcgc aagttccagc agcgtgagaa gaagggcaag     1800

tgcaagaagg cctag                                                      1815


