                         SEQUENCE LISTING

<110>  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS)
 
<120>  CDKACT BIOSENSORS-FLUORESCENT POLYPEPTIDE TO PROBE THE ACTIVITY 
	OF CDK/CYCLIN KINASES IN VITRO, IN CELLULO AND IN VIVO

<130>  364026D30643

<150>  EP12306239.0
<151>  2012-10-10

<160>  35    

<170>  PatentIn version 3.5

<210>  1
<211>  12
<212>  PRT
<213>  artificial

<220>
<223>  Substrate domaine Histone H1

<400>  1

Gly Gly Cys Ser Thr Pro Lys Lys Ala Lys Lys Leu 
1               5                   10          


<210>  2
<211>  16
<212>  PRT
<213>  artificial

<220>
<223>  Substrate domaine Cyclin B1

<400>  2

Pro Glu Pro Ile Leu Val Asp Cys Ser Ser Pro Ser Pro Met Glu Thr 
1               5                   10                  15      


<210>  3
<211>  14
<212>  PRT
<213>  artificial

<220>
<223>  Substrate domain Lamin B2

<400>  3

Arg Ala Gly Gly Pro Ala Thr Cys Ser Ser Pro Thr Arg Leu 
1               5                   10                  


<210>  4
<211>  12
<212>  PRT
<213>  artificial

<220>
<223>  Substrate domain Retinoblastoma protein

<400>  4

Tyr Lys Phe Cys Ser Ser Pro Leu Arg Ile Pro Gly 
1               5                   10          


<210>  5
<211>  15
<212>  PRT
<213>  artificial

<220>
<223>  Substrate domain Tau

<400>  5

Ser Gly Tyr Ser Ser Pro Gly Ser Cys Ser Thr Pro Gly Ser Arg 
1               5                   10                  15  


<210>  6
<211>  603
<212>  PRT
<213>  homo sapiens

<400>  6

Met Ser Ala Ala Val Thr Ala Gly Lys Leu Ala Arg Ala Pro Ala Asp 
1               5                   10                  15      


Pro Gly Lys Ala Gly Val Pro Gly Val Ala Ala Pro Gly Ala Pro Ala 
            20                  25                  30          


Ala Ala Pro Pro Ala Lys Glu Ile Pro Glu Val Leu Val Asp Pro Arg 
        35                  40                  45              


Ser Arg Arg Arg Tyr Val Arg Gly Arg Phe Leu Gly Lys Gly Gly Phe 
    50                  55                  60                  


Ala Lys Cys Phe Glu Ile Ser Asp Ala Asp Thr Lys Glu Val Phe Ala 
65                  70                  75                  80  


Gly Lys Ile Val Pro Lys Ser Leu Leu Leu Lys Pro His Gln Arg Glu 
                85                  90                  95      


Lys Met Ser Met Glu Ile Ser Ile His Arg Ser Leu Ala His Gln His 
            100                 105                 110         


Val Val Gly Phe His Gly Phe Phe Glu Asp Asn Asp Phe Val Phe Val 
        115                 120                 125             


Val Leu Glu Leu Cys Arg Arg Arg Ser Leu Leu Glu Leu His Lys Arg 
    130                 135                 140                 


Arg Lys Ala Leu Thr Glu Pro Glu Ala Arg Tyr Tyr Leu Arg Gln Ile 
145                 150                 155                 160 


Val Leu Gly Cys Gln Tyr Leu His Arg Asn Arg Val Ile His Arg Asp 
                165                 170                 175     


Leu Lys Leu Gly Asn Leu Phe Leu Asn Glu Asp Leu Glu Val Lys Ile 
            180                 185                 190         


Gly Asp Phe Gly Leu Ala Thr Lys Val Glu Tyr Asp Gly Glu Arg Lys 
        195                 200                 205             


Lys Thr Leu Cys Gly Thr Pro Asn Tyr Ile Ala Pro Glu Val Leu Ser 
    210                 215                 220                 


Lys Lys Gly His Ser Phe Glu Val Asp Val Trp Ser Ile Gly Cys Ile 
225                 230                 235                 240 


Met Tyr Thr Leu Leu Val Gly Lys Pro Pro Phe Glu Thr Ser Cys Leu 
                245                 250                 255     


Lys Glu Thr Tyr Leu Arg Ile Lys Lys Asn Glu Tyr Ser Ile Pro Lys 
            260                 265                 270         


His Ile Asn Pro Val Ala Ala Ser Leu Ile Gln Lys Met Leu Gln Thr 
        275                 280                 285             


Asp Pro Thr Ala Arg Pro Thr Ile Asn Glu Leu Leu Asn Asp Glu Phe 
    290                 295                 300                 


Phe Thr Ser Gly Tyr Ile Pro Ala Arg Leu Pro Ile Thr Cys Leu Thr 
305                 310                 315                 320 


Ile Pro Pro Arg Phe Ser Ile Ala Pro Ser Ser Leu Asp Pro Ser Asn 
                325                 330                 335     


Arg Lys Pro Leu Thr Val Leu Asn Lys Gly Leu Glu Asn Pro Leu Pro 
            340                 345                 350         


Glu Arg Pro Arg Glu Lys Glu Glu Pro Val Val Arg Glu Thr Gly Glu 
        355                 360                 365             


Val Val Asp Cys His Leu Ser Asp Met Leu Gln Gln Leu His Ser Val 
    370                 375                 380                 


Asn Ala Ser Lys Pro Ser Glu Arg Gly Leu Val Arg Gln Glu Glu Ala 
385                 390                 395                 400 


Glu Asp Pro Ala Cys Ile Pro Ile Phe Trp Val Ser Lys Trp Val Asp 
                405                 410                 415     


Tyr Ser Asp Lys Tyr Gly Leu Gly Tyr Gln Leu Cys Asp Asn Ser Val 
            420                 425                 430         


Gly Val Leu Phe Asn Asp Ser Thr Arg Leu Ile Leu Tyr Asn Asp Gly 
        435                 440                 445             


Asp Ser Leu Gln Tyr Ile Glu Arg Asp Gly Thr Glu Ser Tyr Leu Thr 
    450                 455                 460                 


Val Ser Ser His Pro Asn Ser Leu Met Lys Lys Ile Thr Leu Leu Lys 
465                 470                 475                 480 


Tyr Phe Arg Asn Tyr Met Ser Glu His Leu Leu Lys Ala Gly Ala Asn 
                485                 490                 495     


Ile Thr Pro Arg Glu Gly Asp Glu Leu Ala Arg Leu Pro Tyr Leu Arg 
            500                 505                 510         


Thr Trp Phe Arg Thr Arg Ser Ala Ile Ile Leu His Leu Ser Asn Gly 
        515                 520                 525             


Ser Val Gln Ile Asn Phe Phe Gln Asp His Thr Lys Leu Ile Leu Cys 
    530                 535                 540                 


Pro Leu Met Ala Ala Val Thr Tyr Ile Asp Glu Lys Arg Asp Phe Arg 
545                 550                 555                 560 


Thr Tyr Arg Leu Ser Leu Leu Glu Glu Tyr Gly Cys Cys Lys Glu Leu 
                565                 570                 575     


Ala Ser Arg Leu Arg Tyr Ala Arg Thr Met Val Asp Lys Leu Leu Ser 
            580                 585                 590         


Ser Arg Ser Ala Ser Asn Arg Leu Lys Ala Ser 
        595                 600             


<210>  7
<211>  162
<212>  PRT
<213>  homo sapiens

<400>  7

Met Ala Asp Glu Glu Lys Leu Pro Pro Gly Trp Glu Lys Arg Met Ser 
1               5                   10                  15      


Arg Ser Ser Gly Arg Val Tyr Tyr Phe Asn His Ile Thr Asn Ala Ser 
            20                  25                  30          


Gln Trp Glu Arg Pro Ser Gly Asn Ser Ser Ser Gly Gly Lys Asn Gly 
        35                  40                  45              


Gln Gly Glu Pro Ala Arg Val Arg Cys Ser His Leu Val Lys His Ser 
    50                  55                  60                  


Gln Ser Arg Arg Pro Ser Ser Trp Arg Gln Glu Lys Ile Thr Arg Thr 
65                  70                  75                  80  


Lys Glu Glu Ala Leu Glu Leu Ile Asn Gly Tyr Ile Gln Lys Ile Lys 
                85                  90                  95      


Ser Gly Glu Glu Asp Phe Glu Ser Leu Ala Ser Gln Phe Ser Asp Cys 
            100                 105                 110         


Ser Ser Ala Lys Ala Arg Gly Asp Leu Gly Ala Phe Ser Arg Gly Gln 
        115                 120                 125             


Met Gln Lys Pro Phe Glu Asp Ala Ser Phe Ala Leu Arg Thr Gly Glu 
    130                 135                 140                 


Met Ser Gly Pro Val Phe Thr Asp Ser Gly Ile His Ile Ile Leu Arg 
145                 150                 155                 160 


Thr Glu 
        


<210>  8
<211>  543
<212>  PRT
<213>  homo sapiens

<400>  8

Met Ser Arg Glu Ser Asp Val Glu Ala Gln Gln Ser His Gly Ser Ser 
1               5                   10                  15      


Ala Cys Ser Gln Pro His Gly Ser Val Thr Gln Ser Gln Gly Ser Ser 
            20                  25                  30          


Ser Gln Ser Gln Gly Ile Ser Ser Ser Ser Thr Ser Thr Met Pro Asn 
        35                  40                  45              


Ser Ser Gln Ser Ser His Ser Ser Ser Gly Thr Leu Ser Ser Leu Glu 
    50                  55                  60                  


Thr Val Ser Thr Gln Glu Leu Tyr Ser Ile Pro Glu Asp Gln Glu Pro 
65                  70                  75                  80  


Glu Asp Gln Glu Pro Glu Glu Pro Thr Pro Ala Pro Trp Ala Arg Leu 
                85                  90                  95      


Trp Ala Leu Gln Asp Gly Phe Ala Asn Leu Glu Cys Val Asn Asp Asn 
            100                 105                 110         


Tyr Trp Phe Gly Arg Asp Lys Ser Cys Glu Tyr Cys Phe Asp Glu Pro 
        115                 120                 125             


Leu Leu Lys Arg Thr Asp Lys Tyr Arg Thr Tyr Ser Lys Lys His Phe 
    130                 135                 140                 


Arg Ile Phe Arg Glu Val Gly Pro Lys Asn Ser Tyr Ile Ala Tyr Ile 
145                 150                 155                 160 


Glu Asp His Ser Gly Asn Gly Thr Phe Val Asn Thr Glu Leu Val Gly 
                165                 170                 175     


Lys Gly Lys Arg Arg Pro Leu Asn Asn Asn Ser Glu Ile Ala Leu Ser 
            180                 185                 190         


Leu Ser Arg Asn Lys Val Phe Val Phe Phe Asp Leu Thr Val Asp Asp 
        195                 200                 205             


Gln Ser Val Tyr Pro Lys Ala Leu Arg Asp Glu Tyr Ile Met Ser Lys 
    210                 215                 220                 


Thr Leu Gly Ser Gly Ala Cys Gly Glu Val Lys Leu Ala Phe Glu Arg 
225                 230                 235                 240 


Lys Thr Cys Lys Lys Val Ala Ile Lys Ile Ile Ser Lys Arg Lys Phe 
                245                 250                 255     


Ala Ile Gly Ser Ala Arg Glu Ala Asp Pro Ala Leu Asn Val Glu Thr 
            260                 265                 270         


Glu Ile Glu Ile Leu Lys Lys Leu Asn His Pro Cys Ile Ile Lys Ile 
        275                 280                 285             


Lys Asn Phe Phe Asp Ala Glu Asp Tyr Tyr Ile Val Leu Glu Leu Met 
    290                 295                 300                 


Glu Gly Gly Glu Leu Phe Asp Lys Val Val Gly Asn Lys Arg Leu Lys 
305                 310                 315                 320 


Glu Ala Thr Cys Lys Leu Tyr Phe Tyr Gln Met Leu Leu Ala Val Gln 
                325                 330                 335     


Tyr Leu His Glu Asn Gly Ile Ile His Arg Asp Leu Lys Pro Glu Asn 
            340                 345                 350         


Val Leu Leu Ser Ser Gln Glu Glu Asp Cys Leu Ile Lys Ile Thr Asp 
        355                 360                 365             


Phe Gly His Ser Lys Ile Leu Gly Glu Thr Ser Leu Met Arg Thr Leu 
    370                 375                 380                 


Cys Gly Thr Pro Thr Tyr Leu Ala Pro Glu Val Leu Val Ser Val Gly 
385                 390                 395                 400 


Thr Ala Gly Tyr Asn Arg Ala Val Asp Cys Trp Ser Leu Gly Val Ile 
                405                 410                 415     


Leu Phe Ile Cys Leu Ser Gly Tyr Pro Pro Phe Ser Glu His Arg Thr 
            420                 425                 430         


Gln Val Ser Leu Lys Asp Gln Ile Thr Ser Gly Lys Tyr Asn Phe Ile 
        435                 440                 445             


Pro Glu Val Trp Ala Glu Val Ser Glu Lys Ala Leu Asp Leu Val Lys 
    450                 455                 460                 


Lys Leu Leu Val Val Asp Pro Lys Ala Arg Phe Thr Thr Glu Glu Ala 
465                 470                 475                 480 


Leu Arg His Pro Trp Leu Gln Asp Glu Asp Met Lys Arg Lys Phe Gln 
                485                 490                 495     


Asp Leu Leu Ser Glu Glu Asn Glu Ser Thr Ala Leu Pro Gln Val Leu 
            500                 505                 510         


Ala Gln Pro Ser Thr Ser Arg Lys Arg Pro Arg Glu Gly Glu Ala Glu 
        515                 520                 525             


Gly Ala Glu Thr Thr Lys Arg Pro Ala Val Cys Ala Ala Val Leu 
    530                 535                 540             


<210>  9
<211>  237
<212>  PRT
<213>  artificial

<220>
<223>  PhosphoBindingDomain of PLK1

<400>  9

Gly Glu Val Val Asp Cys His Leu Ser Asp Met Leu Gln Gln Leu His 
1               5                   10                  15      


Ser Val Asn Ala Ser Lys Pro Ser Glu Arg Gly Leu Val Arg Gln Glu 
            20                  25                  30          


Glu Ala Glu Asp Pro Ala Cys Ile Pro Ile Phe Trp Val Ser Lys Trp 
        35                  40                  45              


Val Asp Tyr Ser Asp Lys Tyr Gly Leu Gly Tyr Gln Leu Cys Asp Asn 
    50                  55                  60                  


Ser Val Gly Val Leu Phe Asn Asp Ser Thr Arg Leu Ile Leu Tyr Asn 
65                  70                  75                  80  


Asp Gly Asp Ser Leu Gln Tyr Ile Glu Arg Asp Gly Thr Glu Ser Tyr 
                85                  90                  95      


Leu Thr Val Ser Ser His Pro Asn Ser Leu Met Lys Lys Ile Thr Leu 
            100                 105                 110         


Leu Lys Tyr Phe Arg Asn Tyr Met Ser Glu His Leu Leu Lys Ala Gly 
        115                 120                 125             


Ala Asn Ile Thr Pro Arg Glu Gly Asp Glu Leu Ala Arg Leu Pro Tyr 
    130                 135                 140                 


Leu Arg Thr Trp Phe Arg Thr Arg Ser Ala Ile Ile Leu His Leu Ser 
145                 150                 155                 160 


Asn Gly Ser Val Gln Ile Asn Phe Phe Gln Asp His Thr Lys Leu Ile 
                165                 170                 175     


Leu Cys Pro Leu Met Ala Ala Val Thr Tyr Ile Asp Glu Lys Arg Asp 
            180                 185                 190         


Phe Arg Thr Tyr Arg Leu Ser Leu Leu Glu Glu Tyr Gly Cys Cys Lys 
        195                 200                 205             


Glu Leu Ala Ser Arg Leu Arg Tyr Ala Arg Thr Met Val Asp Lys Leu 
    210                 215                 220                 


Leu Ser Ser Arg Ser Ala Ser Asn Arg Leu Lys Ala Ser 
225                 230                 235         


<210>  10
<211>  34
<212>  PRT
<213>  artificial

<220>
<223>  WW domain of PIN1

<400>  10

Lys Leu Pro Pro Gly Trp Glu Lys Arg Met Ser Arg Ser Ser Gly Arg 
1               5                   10                  15      


Val Tyr Tyr Phe Asn His Ile Thr Asn Ala Ser Gln Trp Glu Arg Pro 
            20                  25                  30          


Ser Gly 
        


<210>  11
<211>  63
<212>  PRT
<213>  artificial

<220>
<223>  FHA domain of Chk2

<400>  11

Tyr Trp Phe Gly Arg Asp Lys Ser Cys Glu Tyr Cys Phe Asp Glu Pro 
1               5                   10                  15      


Leu Leu Lys Arg Thr Asp Lys Tyr Arg Thr Tyr Ser Lys Lys His Phe 
            20                  25                  30          


Arg Ile Phe Arg Glu Val Gly Pro Lys Asn Ser Tyr Ile Ala Tyr Ile 
        35                  40                  45              


Glu Asp His Ser Gly Asn Gly Thr Phe Val Asn Thr Glu Leu Val 
    50                  55                  60              


<210>  12
<211>  237
<212>  PRT
<213>  artificial

<220>
<223>  Phosphobinding domain of Plk1 protein wherein all Cystein 
       residues are replaced by a Serine residue

<400>  12

Gly Glu Val Val Asp Ser His Leu Ser Asp Met Leu Gln Gln Leu His 
1               5                   10                  15      


Ser Val Asn Ala Ser Lys Pro Ser Glu Arg Gly Leu Val Arg Gln Glu 
            20                  25                  30          


Glu Ala Glu Asp Pro Ala Ser Ile Pro Ile Phe Trp Val Ser Lys Trp 
        35                  40                  45              


Val Asp Tyr Ser Asp Lys Tyr Gly Leu Gly Tyr Gln Leu Ser Asp Asn 
    50                  55                  60                  


Ser Val Gly Val Leu Phe Asn Asp Ser Thr Arg Leu Ile Leu Tyr Asn 
65                  70                  75                  80  


Asp Gly Asp Ser Leu Gln Tyr Ile Glu Arg Asp Gly Thr Glu Ser Tyr 
                85                  90                  95      


Leu Thr Val Ser Ser His Pro Asn Ser Leu Met Lys Lys Ile Thr Leu 
            100                 105                 110         


Leu Lys Tyr Phe Arg Asn Tyr Met Ser Glu His Leu Leu Lys Ala Gly 
        115                 120                 125             


Ala Asn Ile Thr Pro Arg Glu Gly Asp Glu Leu Ala Arg Leu Pro Tyr 
    130                 135                 140                 


Leu Arg Thr Trp Phe Arg Thr Arg Ser Ala Ile Ile Leu His Leu Ser 
145                 150                 155                 160 


Asn Gly Ser Val Gln Ile Asn Phe Phe Gln Asp His Thr Lys Leu Ile 
                165                 170                 175     


Leu Ser Pro Leu Met Ala Ala Val Thr Tyr Ile Asp Glu Lys Arg Asp 
            180                 185                 190         


Phe Arg Thr Tyr Arg Leu Ser Leu Leu Glu Glu Tyr Gly Ser Ser Lys 
        195                 200                 205             


Glu Leu Ala Ser Arg Leu Arg Tyr Ala Arg Thr Met Val Asp Lys Leu 
    210                 215                 220                 


Leu Ser Ser Arg Ser Ala Ser Asn Arg Leu Lys Ala Ser 
225                 230                 235         


<210>  13
<211>  63
<212>  PRT
<213>  artificial

<220>
<223>  FHA domain of Chk2 protein wherein all Cystein residues are 
       replaced by a Serine residue

<400>  13

Tyr Trp Phe Gly Arg Asp Lys Ser Ser Glu Tyr Ser Phe Asp Glu Pro 
1               5                   10                  15      


Leu Leu Lys Arg Thr Asp Lys Tyr Arg Thr Tyr Ser Lys Lys His Phe 
            20                  25                  30          


Arg Ile Phe Arg Glu Val Gly Pro Lys Asn Ser Tyr Ile Ala Tyr Ile 
        35                  40                  45              


Glu Asp His Ser Gly Asn Gly Thr Phe Val Asn Thr Glu Leu Val 
    50                  55                  60              


<210>  14
<211>  12
<212>  PRT
<213>  artificial

<220>
<223>  Linker Sequence

<400>  14

Pro Gly Ala Gly Gly Thr Gly Gly Leu Pro Gly Gly 
1               5                   10          


<210>  15
<211>  258
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide comprising: the phosphobinding domain of Plk1 wherein
       all Cystein residues are replaced by a Serine residue, a linker 
       and the CDK substrate domain from histone H1

<400>  15

Gly Glu Val Val Asp Ser His Leu Ser Asp Met Leu Gln Gln Leu His 
1               5                   10                  15      


Ser Val Asn Ala Ser Lys Pro Ser Glu Arg Gly Leu Val Arg Gln Glu 
            20                  25                  30          


Glu Ala Glu Asp Pro Ala Ser Ile Pro Ile Phe Trp Val Ser Lys Trp 
        35                  40                  45              


Val Asp Tyr Ser Asp Lys Tyr Gly Leu Gly Tyr Gln Leu Ser Asp Asn 
    50                  55                  60                  


Ser Val Gly Val Leu Phe Asn Asp Ser Thr Arg Leu Ile Leu Tyr Asn 
65                  70                  75                  80  


Asp Gly Asp Ser Leu Gln Tyr Ile Glu Arg Asp Gly Thr Glu Ser Tyr 
                85                  90                  95      


Leu Thr Val Ser Ser His Pro Asn Ser Leu Met Lys Lys Ile Thr Leu 
            100                 105                 110         


Leu Lys Tyr Phe Arg Asn Tyr Met Ser Glu Leu Leu Lys Ala Gly Ala 
        115                 120                 125             


Asn Ile Thr Pro Arg Glu Gly Asp Glu Leu Ala Arg Leu Pro Tyr Leu 
    130                 135                 140                 


Arg Thr Trp Phe Arg Thr Arg Ser Ala Ile Ile Leu His Leu Ser Asn 
145                 150                 155                 160 


Gly Ser Val Gln Ile Asn Phe Phe Gln Asp His Thr Lys Leu Ile Leu 
                165                 170                 175     


Ser Pro Leu Met Ala Ala Val Thr Tyr Ile Asp Glu Lys Arg Asp Phe 
            180                 185                 190         


Arg Thr Tyr Arg Leu Ser Leu Leu Glu Glu Tyr Gly Ser Ser Lys Glu 
        195                 200                 205             


Leu Ala Ser Arg Leu Arg Tyr Ala Arg Thr Met Val Asp Lys Leu Leu 
    210                 215                 220                 


Ser Ser Arg Ser Ala Ser Asn Arg Leu Lys Ala Ser Pro Gly Ala Gly 
225                 230                 235                 240 


Gly Thr Gly Gly Leu Pro Gly Gly Cys Ser Thr Pro Lys Lys Ala Lys 
                245                 250                 255     


Lys Leu 
        


<210>  16
<211>  264
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide comprising: the phosphobinding domain of Plk1 wherein
       all Cystein residues are replaced by a Serine residue, a linker 
       and the CDK substrate domain from cyclin B1

<400>  16

Gly Glu Val Val Asp Ser His Leu Ser Asp Met Leu Gln Gln Leu His 
1               5                   10                  15      


Ser Val Asn Ala Ser Lys Pro Ser Glu Arg Gly Leu Val Arg Gln Glu 
            20                  25                  30          


Glu Ala Glu Asp Pro Ala Ser Ile Pro Ile Phe Trp Val Ser Lys Trp 
        35                  40                  45              


Val Asp Tyr Ser Asp Lys Tyr Gly Leu Gly Tyr Gln Leu Ser Asp Asn 
    50                  55                  60                  


Ser Val Gly Val Leu Phe Asn Asp Ser Thr Arg Leu Ile Leu Tyr Asn 
65                  70                  75                  80  


Asp Gly Asp Ser Leu Gln Tyr Ile Glu Arg Asp Gly Thr Glu Ser Tyr 
                85                  90                  95      


Leu Thr Val Ser Ser His Pro Asn Ser Leu Met Lys Lys Ile Thr Leu 
            100                 105                 110         


Leu Lys Tyr Phe Arg Asn Tyr Met Ser Glu Leu Leu Lys Ala Gly Ala 
        115                 120                 125             


Asn Ile Thr Pro Arg Glu Gly Asp Glu Leu Ala Arg Leu Pro Tyr Leu 
    130                 135                 140                 


Arg Thr Trp Phe Arg Thr Arg Ser Ala Ile Ile Leu His Leu Ser Asn 
145                 150                 155                 160 


Gly Ser Val Gln Ile Asn Phe Phe Gln Asp His Thr Lys Leu Ile Leu 
                165                 170                 175     


Ser Pro Leu Met Ala Ala Val Thr Tyr Ile Asp Glu Lys Arg Asp Phe 
            180                 185                 190         


Arg Thr Tyr Arg Leu Ser Leu Leu Glu Glu Tyr Gly Ser Ser Lys Glu 
        195                 200                 205             


Leu Ala Ser Arg Leu Arg Tyr Ala Arg Thr Met Val Asp Lys Leu Leu 
    210                 215                 220                 


Ser Ser Arg Ser Ala Ser Asn Arg Leu Lys Ala Ser Pro Gly Ala Gly 
225                 230                 235                 240 


Gly Thr Gly Gly Leu Pro Gly Gly Pro Glu Pro Ile Leu Val Asp Cys 
                245                 250                 255     


Ser Ser Pro Ser Pro Met Glu Thr 
            260                 


<210>  17
<211>  262
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide comprising: the phosphobinding domain of Plk1 wherein
       all Cystein residues are replaced by a Serine residue, a linker 
       and the CDK substrate domain from Lamin B2

<400>  17

Gly Glu Val Val Asp Ser His Leu Ser Asp Met Leu Gln Gln Leu His 
1               5                   10                  15      


Ser Val Asn Ala Ser Lys Pro Ser Glu Arg Gly Leu Val Arg Gln Glu 
            20                  25                  30          


Glu Ala Glu Asp Pro Ala Ser Ile Pro Ile Phe Trp Val Ser Lys Trp 
        35                  40                  45              


Val Asp Tyr Ser Asp Lys Tyr Gly Leu Gly Tyr Gln Leu Ser Asp Asn 
    50                  55                  60                  


Ser Val Gly Val Leu Phe Asn Asp Ser Thr Arg Leu Ile Leu Tyr Asn 
65                  70                  75                  80  


Asp Gly Asp Ser Leu Gln Tyr Ile Glu Arg Asp Gly Thr Glu Ser Tyr 
                85                  90                  95      


Leu Thr Val Ser Ser His Pro Asn Ser Leu Met Lys Lys Ile Thr Leu 
            100                 105                 110         


Leu Lys Tyr Phe Arg Asn Tyr Met Ser Glu Leu Leu Lys Ala Gly Ala 
        115                 120                 125             


Asn Ile Thr Pro Arg Glu Gly Asp Glu Leu Ala Arg Leu Pro Tyr Leu 
    130                 135                 140                 


Arg Thr Trp Phe Arg Thr Arg Ser Ala Ile Ile Leu His Leu Ser Asn 
145                 150                 155                 160 


Gly Ser Val Gln Ile Asn Phe Phe Gln Asp His Thr Lys Leu Ile Leu 
                165                 170                 175     


Ser Pro Leu Met Ala Ala Val Thr Tyr Ile Asp Glu Lys Arg Asp Phe 
            180                 185                 190         


Arg Thr Tyr Arg Leu Ser Leu Leu Glu Glu Tyr Gly Ser Ser Lys Glu 
        195                 200                 205             


Leu Ala Ser Arg Leu Arg Tyr Ala Arg Thr Met Val Asp Lys Leu Leu 
    210                 215                 220                 


Ser Ser Arg Ser Ala Ser Asn Arg Leu Lys Ala Ser Pro Gly Ala Gly 
225                 230                 235                 240 


Gly Thr Gly Gly Leu Pro Gly Gly Arg Ala Gly Gly Pro Ala Thr Cys 
                245                 250                 255     


Ser Ser Pro Thr Arg Leu 
            260         


<210>  18
<211>  260
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide comprising: the phosphobinding domain of Plk1 wherein
       all Cystein residues are replaced by a Serine residue, a linker 
       and the CDK substrate domain from Retinoblastoma

<400>  18

Gly Glu Val Val Asp Ser His Leu Ser Asp Met Leu Gln Gln Leu His 
1               5                   10                  15      


Ser Val Asn Ala Ser Lys Pro Ser Glu Arg Gly Leu Val Arg Gln Glu 
            20                  25                  30          


Glu Ala Glu Asp Pro Ala Ser Ile Pro Ile Phe Trp Val Ser Lys Trp 
        35                  40                  45              


Val Asp Tyr Ser Asp Lys Tyr Gly Leu Gly Tyr Gln Leu Ser Asp Asn 
    50                  55                  60                  


Ser Val Gly Val Leu Phe Asn Asp Ser Thr Arg Leu Ile Leu Tyr Asn 
65                  70                  75                  80  


Asp Gly Asp Ser Leu Gln Tyr Ile Glu Arg Asp Gly Thr Glu Ser Tyr 
                85                  90                  95      


Leu Thr Val Ser Ser His Pro Asn Ser Leu Met Lys Lys Ile Thr Leu 
            100                 105                 110         


Leu Lys Tyr Phe Arg Asn Tyr Met Ser Glu Leu Leu Lys Ala Gly Ala 
        115                 120                 125             


Asn Ile Thr Pro Arg Glu Gly Asp Glu Leu Ala Arg Leu Pro Tyr Leu 
    130                 135                 140                 


Arg Thr Trp Phe Arg Thr Arg Ser Ala Ile Ile Leu His Leu Ser Asn 
145                 150                 155                 160 


Gly Ser Val Gln Ile Asn Phe Phe Gln Asp His Thr Lys Leu Ile Leu 
                165                 170                 175     


Ser Pro Leu Met Ala Ala Val Thr Tyr Ile Asp Glu Lys Arg Asp Phe 
            180                 185                 190         


Arg Thr Tyr Arg Leu Ser Leu Leu Glu Glu Tyr Gly Ser Ser Lys Glu 
        195                 200                 205             


Leu Ala Ser Arg Leu Arg Tyr Ala Arg Thr Met Val Asp Lys Leu Leu 
    210                 215                 220                 


Ser Ser Arg Ser Ala Ser Asn Arg Leu Lys Ala Ser Pro Gly Ala Gly 
225                 230                 235                 240 


Gly Thr Gly Gly Leu Pro Gly Gly Tyr Lys Phe Cys Ser Ser Pro Leu 
                245                 250                 255     


Arg Ile Pro Gly 
            260 


<210>  19
<211>  263
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide comprising: the phosphobinding domain of Plk1 wherein
       all Cystein residues are replaced by a Serine residue, a linker 
       and the CDK substrate domain from Tau

<400>  19

Gly Glu Val Val Asp Ser His Leu Ser Asp Met Leu Gln Gln Leu His 
1               5                   10                  15      


Ser Val Asn Ala Ser Lys Pro Ser Glu Arg Gly Leu Val Arg Gln Glu 
            20                  25                  30          


Glu Ala Glu Asp Pro Ala Ser Ile Pro Ile Phe Trp Val Ser Lys Trp 
        35                  40                  45              


Val Asp Tyr Ser Asp Lys Tyr Gly Leu Gly Tyr Gln Leu Ser Asp Asn 
    50                  55                  60                  


Ser Val Gly Val Leu Phe Asn Asp Ser Thr Arg Leu Ile Leu Tyr Asn 
65                  70                  75                  80  


Asp Gly Asp Ser Leu Gln Tyr Ile Glu Arg Asp Gly Thr Glu Ser Tyr 
                85                  90                  95      


Leu Thr Val Ser Ser His Pro Asn Ser Leu Met Lys Lys Ile Thr Leu 
            100                 105                 110         


Leu Lys Tyr Phe Arg Asn Tyr Met Ser Glu Leu Leu Lys Ala Gly Ala 
        115                 120                 125             


Asn Ile Thr Pro Arg Glu Gly Asp Glu Leu Ala Arg Leu Pro Tyr Leu 
    130                 135                 140                 


Arg Thr Trp Phe Arg Thr Arg Ser Ala Ile Ile Leu His Leu Ser Asn 
145                 150                 155                 160 


Gly Ser Val Gln Ile Asn Phe Phe Gln Asp His Thr Lys Leu Ile Leu 
                165                 170                 175     


Ser Pro Leu Met Ala Ala Val Thr Tyr Ile Asp Glu Lys Arg Asp Phe 
            180                 185                 190         


Arg Thr Tyr Arg Leu Ser Leu Leu Glu Glu Tyr Gly Ser Ser Lys Glu 
        195                 200                 205             


Leu Ala Ser Arg Leu Arg Tyr Ala Arg Thr Met Val Asp Lys Leu Leu 
    210                 215                 220                 


Ser Ser Arg Ser Ala Ser Asn Arg Leu Lys Ala Ser Pro Gly Ala Gly 
225                 230                 235                 240 


Gly Thr Gly Gly Leu Pro Gly Gly Ser Gly Tyr Ser Ser Pro Gly Ser 
                245                 250                 255     


Cys Ser Thr Pro Gly Ser Arg 
            260             


<210>  20
<211>  58
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide comprising: the WW domain of PIN1 wherein all Cystein
       residues are replaced by a Serine residue, a linker and the CDK 
       substrate domain from histone H1

<400>  20

Lys Leu Pro Pro Gly Trp Glu Lys Arg Met Ser Arg Ser Ser Gly Arg 
1               5                   10                  15      


Val Tyr Tyr Phe Asn His Ile Thr Asn Ala Ser Gln Trp Glu Arg Pro 
            20                  25                  30          


Ser Gly Pro Gly Ala Gly Gly Thr Gly Gly Leu Pro Gly Gly Gly Gly 
        35                  40                  45              


Cys Ser Thr Pro Lys Lys Ala Lys Lys Leu 
    50                  55              


<210>  21
<211>  62
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide comprising: the WW domain of PIN1 wherein all Cystein
       residues are replaced by a Serine residue, a linker and the CDK 
       substrate domain from cyclin B1

<400>  21

Lys Leu Pro Pro Gly Trp Glu Lys Arg Met Ser Arg Ser Ser Gly Arg 
1               5                   10                  15      


Val Tyr Tyr Phe Asn His Ile Thr Asn Ala Ser Gln Trp Glu Arg Pro 
            20                  25                  30          


Ser Gly Pro Gly Ala Gly Gly Thr Gly Gly Leu Pro Gly Gly Pro Glu 
        35                  40                  45              


Pro Ile Leu Val Asp Cys Ser Ser Pro Ser Pro Met Glu Thr 
    50                  55                  60          


<210>  22
<211>  60
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide comprising: the WW domain of PIN1 wherein all Cystein
       residues are replaced by a Serine residue, a linker and the CDK 
       substrate domain from Lamin B2

<400>  22

Lys Leu Pro Pro Gly Trp Glu Lys Arg Met Ser Arg Ser Ser Gly Arg 
1               5                   10                  15      


Val Tyr Tyr Phe Asn His Ile Thr Asn Ala Ser Gln Trp Glu Arg Pro 
            20                  25                  30          


Ser Gly Pro Gly Ala Gly Gly Thr Gly Gly Leu Pro Gly Gly Arg Ala 
        35                  40                  45              


Gly Gly Pro Ala Thr Cys Ser Ser Pro Thr Arg Leu 
    50                  55                  60  


<210>  23
<211>  58
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide comprising: the WW domain of PIN1 wherein all Cystein
       residues are replaced by a Serine residue, a linker and the CDK 
       substrate domain from Retinoblastoma

<400>  23

Lys Leu Pro Pro Gly Trp Glu Lys Arg Met Ser Arg Ser Ser Gly Arg 
1               5                   10                  15      


Val Tyr Tyr Phe Asn His Ile Thr Asn Ala Ser Gln Trp Glu Arg Pro 
            20                  25                  30          


Ser Gly Pro Gly Ala Gly Gly Thr Gly Gly Leu Pro Gly Gly Tyr Lys 
        35                  40                  45              


Phe Cys Ser Ser Pro Leu Arg Ile Pro Gly 
    50                  55              


<210>  24
<211>  61
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide comprising: the WW domain of PIN1 wherein all Cystein
       residues are replaced by a Serine residue, a linker and the CDK 
       substrate domain from Tau

<400>  24

Lys Leu Pro Pro Gly Trp Glu Lys Arg Met Ser Arg Ser Ser Gly Arg 
1               5                   10                  15      


Val Tyr Tyr Phe Asn His Ile Thr Asn Ala Ser Gln Trp Glu Arg Pro 
            20                  25                  30          


Ser Gly Pro Gly Ala Gly Gly Thr Gly Gly Leu Pro Gly Gly Ser Gly 
        35                  40                  45              


Tyr Ser Ser Pro Gly Ser Cys Ser Thr Pro Gly Ser Arg 
    50                  55                  60      


<210>  25
<211>  87
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide comprising: the FHA domain of Chk2 wherein all 
       Cystein residues are replaced by a Serine residue, a linker and 
       the CDK substrate domain from histone H1

<400>  25

Tyr Trp Phe Gly Arg Asp Lys Ser Ser Glu Tyr Ser Phe Asp Glu Pro 
1               5                   10                  15      


Leu Leu Lys Arg Thr Asp Lys Tyr Arg Thr Tyr Ser Lys Lys His Phe 
            20                  25                  30          


Arg Ile Phe Arg Glu Val Gly Pro Lys Asn Ser Tyr Ile Ala Tyr Ile 
        35                  40                  45              


Glu Asp His Ser Gly Asn Gly Thr Phe Val Asn Thr Glu Leu Val Pro 
    50                  55                  60                  


Gly Ala Gly Gly Thr Gly Gly Leu Pro Gly Gly Gly Gly Cys Ser Thr 
65                  70                  75                  80  


Pro Lys Lys Ala Lys Lys Leu 
                85          


<210>  26
<211>  91
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide comprising: the FHA domain of Chk2 wherein all 
       Cystein residues are replaced by a Serine residue, a linker and 
       the CDK substrate domain from cyclin B1

<400>  26

Tyr Trp Phe Gly Arg Asp Lys Ser Ser Glu Tyr Ser Phe Asp Glu Pro 
1               5                   10                  15      


Leu Leu Lys Arg Thr Asp Lys Tyr Arg Thr Tyr Ser Lys Lys His Phe 
            20                  25                  30          


Arg Ile Phe Arg Glu Val Gly Pro Lys Asn Ser Tyr Ile Ala Tyr Ile 
        35                  40                  45              


Glu Asp His Ser Gly Asn Gly Thr Phe Val Asn Thr Glu Leu Val Pro 
    50                  55                  60                  


Gly Ala Gly Gly Thr Gly Gly Leu Pro Gly Gly Pro Glu Pro Ile Leu 
65                  70                  75                  80  


Val Asp Cys Ser Ser Pro Ser Pro Met Glu Thr 
                85                  90      


<210>  27
<211>  89
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide comprising: the FHA domain of Chk2 wherein all 
       Cystein residues are replaced by a Serine residue, a linker and 
       the CDK substrate domain from Lamin B2

<400>  27

Tyr Trp Phe Gly Arg Asp Lys Ser Ser Glu Tyr Ser Phe Asp Glu Pro 
1               5                   10                  15      


Leu Leu Lys Arg Thr Asp Lys Tyr Arg Thr Tyr Ser Lys Lys His Phe 
            20                  25                  30          


Arg Ile Phe Arg Glu Val Gly Pro Lys Asn Ser Tyr Ile Ala Tyr Ile 
        35                  40                  45              


Glu Asp His Ser Gly Asn Gly Thr Phe Val Asn Thr Glu Leu Val Pro 
    50                  55                  60                  


Gly Ala Gly Gly Thr Gly Gly Leu Pro Gly Gly Arg Ala Gly Gly Pro 
65                  70                  75                  80  


Ala Thr Cys Ser Ser Pro Thr Arg Leu 
                85                  


<210>  28
<211>  87
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide comprising: the FHA domain of Chk2 wherein all 
       Cystein residues are replaced by a Serine residue, a linker and 
       the CDK substrate domain from Retinoblastoma

<400>  28

Tyr Trp Phe Gly Arg Asp Lys Ser Ser Glu Tyr Ser Phe Asp Glu Pro 
1               5                   10                  15      


Leu Leu Lys Arg Thr Asp Lys Tyr Arg Thr Tyr Ser Lys Lys His Phe 
            20                  25                  30          


Arg Ile Phe Arg Glu Val Gly Pro Lys Asn Ser Tyr Ile Ala Tyr Ile 
        35                  40                  45              


Glu Asp His Ser Gly Asn Gly Thr Phe Val Asn Thr Glu Leu Val Pro 
    50                  55                  60                  


Gly Ala Gly Gly Thr Gly Gly Leu Pro Gly Gly Tyr Lys Phe Cys Ser 
65                  70                  75                  80  


Ser Pro Leu Arg Ile Pro Gly 
                85          


<210>  29
<211>  90
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide comprising: the FHA domain of Chk2 wherein all 
       Cystein residues are replaced by a Serine residue, a linker and 
       the CDK substrate domain from Tau

<400>  29

Tyr Trp Phe Gly Arg Asp Lys Ser Ser Glu Tyr Ser Phe Asp Glu Pro 
1               5                   10                  15      


Leu Leu Lys Arg Thr Asp Lys Tyr Arg Thr Tyr Ser Lys Lys His Phe 
            20                  25                  30          


Arg Ile Phe Arg Glu Val Gly Pro Lys Asn Ser Tyr Ile Ala Tyr Ile 
        35                  40                  45              


Glu Asp His Ser Gly Asn Gly Thr Phe Val Asn Thr Glu Leu Val Pro 
    50                  55                  60                  


Gly Ala Gly Gly Thr Gly Gly Leu Pro Gly Gly Ser Gly Tyr Ser Ser 
65                  70                  75                  80  


Pro Gly Ser Cys Ser Thr Pro Gly Ser Arg 
                85                  90  


<210>  30
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  Consensus phosphorylated sequence from the CDK substrate domain


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (3)..(3)
<223>  Ser may be replaces by Thr

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  Xaa can be any naturally occurring amino acid

<400>  30

Xaa Ser Ser Pro Xaa 
1               5   


<210>  31
<211>  261
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide comprising: the phosphobinding domain of Plk1 wherein
       all Cystein residues are replaced by a Serine residue, a linker 
       and the CDK substrate domain from histone H1

Gly Glu Val Val Asp Ser His Leu Ser Asp Met Leu Gln Gln Leu His 
1               5                   10                  15      


Ser Val Asn Ala Ser Lys Pro Ser Glu Arg Gly Leu Val Arg Gln Glu 
            20                  25                  30          


Glu Ala Glu Asp Pro Ala Ser Ile Pro Ile Phe Trp Val Ser Lys Trp 
        35                  40                  45              


Val Asp Tyr Ser Asp Lys Tyr Gly Leu Gly Tyr Gln Leu Ser Asp Asn 
    50                  55                  60                  


Ser Val Gly Val Leu Phe Asn Asp Ser Thr Arg Leu Ile Leu Tyr Asn 
65                  70                  75                  80  


Asp Gly Asp Ser Leu Gln Tyr Ile Glu Arg Asp Gly Thr Glu Ser Tyr 
                85                  90                  95      


Leu Thr Val Ser Ser His Pro Asn Ser Leu Met Lys Lys Ile Thr Leu 
            100                 105                 110         


Leu Lys Tyr Phe Arg Asn Tyr Met Ser Glu His Leu Leu Lys Ala Gly  
        115                 120                 125             


Ala Asn Ile Thr Pro Arg Glu Gly Asp Glu Leu Ala Arg Leu Pro Tyr  
    130                 135                 140                 


Leu Arg Thr Trp Phe Arg Thr Arg Ser Ala Ile Ile Leu His Leu Ser  
145                 150                 155                 160 


Asn Gly Ser Val Gln Ile Asn Phe Phe Gln Asp His Thr Lys Leu Ile  
                165                 170                 175     


Leu Ser Pro Leu Met Ala Ala Val Thr Tyr Ile Asp Glu Lys Arg Asp  
            180                 185                 190         


Phe Arg Thr Tyr Arg Leu Ser Leu Leu Glu Glu Tyr Gly Ser Ser Lys  
        195                 200                 205             


Glu Leu Ala Ser Arg Leu Arg Tyr Ala Arg Thr Met Val Asp Lys Leu 
    210                 215                 220                 


Leu Ser Ser Arg Ser Ala Ser Asn Arg Leu Lys Ala Ser Pro Gly Ala  
225                 230                 235                 240 


Gly Gly Thr Gly Gly Leu Pro Gly Gly Gly Gly Cys Ser Thr Pro Lys  
                245                 250                 255     


Lys Ala Lys Lys Leu 
            260




<210>  32
<211>  265
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide comprising: the phosphobinding domain of Plk1 wherein
       all Cystein residues are replaced by a Serine residue, a linker 
       and the CDK substrate domain from cyclin B1

Gly Glu Val Val Asp Ser His Leu Ser Asp Met Leu Gln Gln Leu His 
1               5                   10                  15      


Ser Val Asn Ala Ser Lys Pro Ser Glu Arg Gly Leu Val Arg Gln Glu 
            20                  25                  30          


Glu Ala Glu Asp Pro Ala Ser Ile Pro Ile Phe Trp Val Ser Lys Trp 
        35                  40                  45              


Val Asp Tyr Ser Asp Lys Tyr Gly Leu Gly Tyr Gln Leu Ser Asp Asn 
    50                  55                  60                  


Ser Val Gly Val Leu Phe Asn Asp Ser Thr Arg Leu Ile Leu Tyr Asn 
65                  70                  75                  80  


Asp Gly Asp Ser Leu Gln Tyr Ile Glu Arg Asp Gly Thr Glu Ser Tyr 
                85                  90                  95      


Leu Thr Val Ser Ser His Pro Asn Ser Leu Met Lys Lys Ile Thr Leu 
            100                 105                 110         


Leu Lys Tyr Phe Arg Asn Tyr Met Ser His Glu Leu Leu Lys Ala Gly  
        115                 120                 125             


Ala Asn Ile Thr Pro Arg Glu Gly Asp Glu Leu Ala Arg Leu Pro Tyr  
    130                 135                 140                 


Leu Arg Thr Trp Phe Arg Thr Arg Ser Ala Ile Ile Leu His Leu Ser  
145                 150                 155                 160 


Asn Gly Ser Val Gln Ile Asn Phe Phe Gln Asp His Thr Lys Leu Ile  
                165                 170                 175     


Leu Ser Pro Leu Met Ala Ala Val Thr Tyr Ile Asp Glu Lys Arg Asp  
            180                 185                 190         


Phe Arg Thr Tyr Arg Leu Ser Leu Leu Glu Glu Tyr Gly Ser Ser Lys  
        195                 200                 205             


Glu Leu Ala Ser Arg Leu Arg Tyr Ala Arg Thr Met Val Asp Lys Leu  
    210                 215                 220                 


Leu Ser Ser Arg Ser Ala Ser Asn Arg Leu Lys Ala Ser Pro Gly Ala  
225                 230                 235                 240 


Gly Gly Thr Gly Gly Leu Pro Gly Gly Pro Glu Pro Ile Leu Val Asp  
                245                 250                 255     


Cys Ser Ser Pro Ser Pro Met Glu Thr 
            260                 265




<210>  33
<211>  263
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide comprising: the phosphobinding domain of Plk1 wherein
       all Cystein residues are replaced by a Serine residue, a linker 
       and the CDK substrate domain from Lamin B2

Gly Glu Val Val Asp Ser His Leu Ser Asp Met Leu Gln Gln Leu His 
1               5                   10                  15      


Ser Val Asn Ala Ser Lys Pro Ser Glu Arg Gly Leu Val Arg Gln Glu 
            20                  25                  30          


Glu Ala Glu Asp Pro Ala Ser Ile Pro Ile Phe Trp Val Ser Lys Trp 
        35                  40                  45              


Val Asp Tyr Ser Asp Lys Tyr Gly Leu Gly Tyr Gln Leu Ser Asp Asn 
    50                  55                  60                  


Ser Val Gly Val Leu Phe Asn Asp Ser Thr Arg Leu Ile Leu Tyr Asn 
65                  70                  75                  80  


Asp Gly Asp Ser Leu Gln Tyr Ile Glu Arg Asp Gly Thr Glu Ser Tyr 
                85                  90                  95      


Leu Thr Val Ser Ser His Pro Asn Ser Leu Met Lys Lys Ile Thr Leu 
            100                 105                 110         


Leu Lys Tyr Phe Arg Asn Tyr Met Ser Glu His Leu Leu Lys Ala Gly 
        115                 120                 125             


Ala Asn Ile Thr Pro Arg Glu Gly Asp Glu Leu Ala Arg Leu Pro Tyr  
    130                 135                 140                 


Leu Arg Thr Trp Phe Arg Thr Arg Ser Ala Ile Ile Leu His Leu Ser 


Asn Gly Ser Val Gln Ile Asn Phe Phe Gln Asp His Thr Lys Leu Ile  
                165                 170                 175     


Leu Ser Pro Leu Met Ala Ala Val Thr Tyr Ile Asp Glu Lys Arg Asp  
            180                 185                 190         


Phe Arg Thr Tyr Arg Leu Ser Leu Leu Glu Glu Tyr Gly Ser Ser Lys  
        195                 200                 205             


Glu Leu Ala Ser Arg Leu Arg Tyr Ala Arg Thr Met Val Asp Lys Leu  
    210                 215                 220                 


Leu Ser Ser Arg Ser Ala Ser Asn Arg Leu Lys Ala Ser Pro Gly Ala  
225                 230                 235                 240 


Gly Gly Thr Gly Gly Leu Pro Gly Gly Arg Ala Gly Gly Pro Ala Thr  
                245                 250                 255     


Cys Ser Ser Pro Thr Arg Leu 
            260         




<210>  34
<211>  261
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide comprising: the phosphobinding domain of Plk1 wherein
       all Cystein residues are replaced by a Serine residue, a linker 
       and the CDK substrate domain from retinoblastoma

Gly Glu Val Val Asp Ser His Leu Ser Asp Met Leu Gln Gln Leu His 
1               5                   10                  15      


Ser Val Asn Ala Ser Lys Pro Ser Glu Arg Gly Leu Val Arg Gln Glu 
            20                  25                  30          


Glu Ala Glu Asp Pro Ala Ser Ile Pro Ile Phe Trp Val Ser Lys Trp 
        35                  40                  45              


Val Asp Tyr Ser Asp Lys Tyr Gly Leu Gly Tyr Gln Leu Ser Asp Asn 
    50                  55                  60                  


Ser Val Gly Val Leu Phe Asn Asp Ser Thr Arg Leu Ile Leu Tyr Asn 
65                  70                  75                  80  


Asp Gly Asp Ser Leu Gln Tyr Ile Glu Arg Asp Gly Thr Glu Ser Tyr 
                85                  90                  95      


Leu Thr Val Ser Ser His Pro Asn Ser Leu Met Lys Lys Ile Thr Leu 
            100                 105                 110         


Leu Lys Tyr Phe Arg Asn Tyr Met Ser Glu His Leu Leu Lys Ala Gly  
        115                 120                 125             


Ala Asn Ile Thr Pro Arg Glu Gly Asp Glu Leu Ala Arg Leu Pro Tyr  
    130                 135                 140                 


Leu Arg Thr Trp Phe Arg Thr Arg Ser Ala Ile Ile Leu His Leu Ser  
145                 150                 155                 160 


Asn Gly Ser Val Gln Ile Asn Phe Phe Gln Asp His Thr Lys Leu Ile  
                165                 170                 175     


Leu Ser Pro Leu Met Ala Ala Val Thr Tyr Ile Asp Glu Lys Arg Asp  
            180                 185                 190         


Phe Arg Thr Tyr Arg Leu Ser Leu Leu Glu Glu Tyr Gly Ser Ser Lys  
        195                 200                 205             


Glu Leu Ala Ser Arg Leu Arg Tyr Ala Arg Thr Met Val Asp Lys Leu 
    210                 215                 220                 


Leu Ser Ser Arg Ser Ala Ser Asn Arg Leu Lys Ala Ser Pro Gly Ala  
225                 230                 235                 240 


Gly Gly Thr Gly Gly Leu Pro Gly Gly Tyr Lys Phe Cys Ser Ser Pro  
                245                 250                 255     


Leu Arg Ile Pro Gly 
            260 



<210>  35
<211>  264
<212>  PRT
<213>  artificial

<220>
<223>  Polypeptide comprising: the phosphobinding domain of Plk1 wherein
       all Cystein residues are replaced by a Serine residue, a linker 
       and the CDK substrate domain from Tau

Gly Glu Val Val Asp Ser His Leu Ser Asp Met Leu Gln Gln Leu His 
1               5                   10                  15      


Ser Val Asn Ala Ser Lys Pro Ser Glu Arg Gly Leu Val Arg Gln Glu 
            20                  25                  30          


Glu Ala Glu Asp Pro Ala Ser Ile Pro Ile Phe Trp Val Ser Lys Trp 
        35                  40                  45              


Val Asp Tyr Ser Asp Lys Tyr Gly Leu Gly Tyr Gln Leu Ser Asp Asn 
    50                  55                  60                  


Ser Val Gly Val Leu Phe Asn Asp Ser Thr Arg Leu Ile Leu Tyr Asn 
65                  70                  75                  80  


Asp Gly Asp Ser Leu Gln Tyr Ile Glu Arg Asp Gly Thr Glu Ser Tyr 
                85                  90                  95      


Leu Thr Val Ser Ser His Pro Asn Ser Leu Met Lys Lys Ile Thr Leu 
            100                 105                 110         


Leu Lys Tyr Phe Arg Asn Tyr Met Ser Glu His Leu Leu Lys Ala Gly  
        115                 120                 125             


Ala Asn Ile Thr Pro Arg Glu Gly Asp Glu Leu Ala Arg Leu Pro Tyr  
    130                 135                 140                 


Leu Arg Thr Trp Phe Arg Thr Arg Ser Ala Ile Ile Leu His Leu Ser  
145                 150                 155                 160 


Asn Gly Ser Val Gln Ile Asn Phe Phe Gln Asp His Thr Lys Leu Ile  
                165                 170                 175     


Leu Ser Pro Leu Met Ala Ala Val Thr Tyr Ile Asp Glu Lys Arg Asp  
            180                 185                 190         


Phe Arg Thr Tyr Arg Leu Ser Leu Leu Glu Glu Tyr Gly Ser Ser Lys  
        195                 200                 205             


Glu Leu Ala Ser Arg Leu Arg Tyr Ala Arg Thr Met Val Asp Lys Leu  
    210                 215                 220                 


Leu Ser Ser Arg Ser Ala Ser Asn Arg Leu Lys Ala Ser Pro Gly Ala  
225                 230                 235                 240 


Gly Gly Thr Gly Gly Leu Pro Gly Gly Ser Gly Tyr Ser Ser Pro Gly  
                245                 250                 255     


Ser Cys Ser Thr Pro Gly Ser Arg 
            260             

