SEQUENCE LISTING

<110> MATHIS LUC
	VOYTAS DAN
	ZHANG FENG
	LI JIN
	LUO SONG
		
 
<120>  COUPLING HERBICIDE RESISTANCE WITH TARGETED INSERTION OF TRANSGENES IN PLANTS
	
<130>  407049WO (DI2012-15PCT)

<160>  13   

<170>  PatentIn version 3.5


<210>  1

<211>  259
<212>  DNA
<213>  artificial sequence

<220>  	
<223>  description of artificial sequence: synthetic oligonucleotide

<220>
<223>  ALS1_T01

<400>  1
gattaatttc tagtggagta gtttagtgcg ataaagttag cttgtttcca catttttatt     60
tcgtaagcta tgtcagccag ggtcagattg gaactaaagg tgttaaatgg gtgggtcggg    120
ccgggcttct attttttgga cttgtatggg ttacgatccg ggcctgttat aagttgattc    180
ttaatggctt cgggttcatc cgggtaaaaa ttgaaccata agggttactg gttgaggggg    240
ccggatcgtg ccgggttta                                                 259


<210>  2

<211>  259
<212>  DNA
<213>  artificial sequence

<220>  	
<223>  description of artificial sequence: synthetic oligonucleotide

<220>
<223>  ALS1_T02

<400>  2
gattaatttc tagtggagta gtttagtgcg ataaagttag cttgtttcca catttttatt     60
tcgtaagcta tgtcagccag ggtcagattg gaactaaagg tgttaaatgg gtgggtcggg    120
ccgggcttct attttttgga cttgtatggg ttacgatccg ggcctgttat aagttgattc    180
ttaatggctt cgggttcatc cgggtaaaaa ttgaaccata agggttactg gttgaggggg    240
ccggatcgtg ccgggttta                                                 259


<210>  3

<211>  264
<212>  DNA
<213>  artificial sequence

<220>  	
<223>  description of artificial sequence: synthetic oligonucleotide

<220>
<223>  ALS2_T01

<400>  3
gattaatttc taatggagta gtttagtgta ataaagttag cttgttccac atttttattt     60
cataagctat gtcatgctgg gtcagattgg aacttcctct ttaggttgga tgtaatccct    120
attagggctt tctcttaatt ttattattga attgttggct tttaatctga gcaagttgat    180
ttgcagcttt ctctcgagtc ctaggagcaa tacgttatct ctgtctccta tttcctagtg    240
gataatctta tgatggaaat atgt                                           264


<210>  4

<211>  264
<212>  DNA
<213>  artificial sequence

<220>  	
<223>  description of artificial sequence: synthetic oligonucleotide

<220>
<223>  ALS2_T02

<400>  4
gattaatttc taatggagta gtttagtgta ataaagttag cttgttccac atttttattt     60
cataagctat gtcatgctgg gtcagattgg aacttcctct ttaggttgga tgtaatccct    120
attagggctt tctcttaatt ttattattga attgttggct tttaatctga gcaagttgat    180
ttgcagcttt ctctcgagtc ctaggagcaa tacgttatct ctgtctccta tttcctagtg    240
gataatctta tgatggaaat atgt                                           264


<210>  5

<211>  5017
<212>  DNA
<213>  artificial sequence

<220>  	
<223>  description of artificial sequence: synthetic DNA

<220>
<223>  Donor sequence for ALS1 knock-in

<400>  5
gaattcacta ttggaaagta aggaaggtaa actgaagttg gatttttctg cttggaggca     60
ggagttgacg gagcagaaag tgaagcaccc gttgaacttt aaaacttttg gtgatgctat    120
tcctccgcaa tatgctatcc aggttctaga tgagttaact aatgggaatg ctattataag    180
tactggtgtg gggcaacacc agatgtgggc tgctcagtac tataagtaca gaaagccacg    240
ccaatggttg acatctggtg gattaggagc aatgggattt ggtttgcctg ctgctattgg    300
tgcagctgtt ggaagaccgg atgaagttgt ggttgacatt gatggcgatg gcagtttcat    360
catgaatgtg caggagcttg caacaattaa ggtggagaat ctcccagtta agattatgtt    420
gctgaataat caacacttgg gaatggtggt tcaactcgag gatcggttct ataaggctaa    480
cagagcacac acatacctgg ggaatccttc taatgaggcg gagattttcc ctaacatgtt    540
gaaatttgca gaggcttgtg gtgtacctgc tgcaagagtg acacataggg atgatcttag    600
agctgccatt cagaagatgt tagacactcc tgggccatac ttgttggacg tgattgtacc    660
tcatcaggaa catgttctac ctatgattcc cagtggcgga gctttcaaag atgtgatcac    720
agagggtgat gggagaagtt cctattgagt ttgagaagct gcagagctag ttctagacct    780
tgtattatct gaaataaact tctattaagc caaacatgtt ctgtctatca gtttgttatt    840
agtttttgcc gtggctttgc tcattgtcac tgttgtacta ttaagtaggg ttagttgata    900
tttatgattg ctttaagttt tgcatcatct ccctttggtt ttgaatgtga aggatttcag    960
caaagtttca ttctctattt gcaacatcca cttggtatct ggagattaat ttctagtgga   1020
gtagtttagt gcgataaagt tagcttgttt ccacattttt atttcgtaag ctatgtcagc   1080
cagggtcaga ttggaactaa aggtgttaaa tgggtgggtc gggccgggct tctatttact   1140
agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc gaacagttca   1200
tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg gtggagcacg   1260
acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa agggcaattg   1320
agacttttca acaaagggta atatccggaa acctcctcgg attccattgc ccagctatct   1380
gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc catcattgcg   1440
ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa gatggacccc   1500
cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg   1560
attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag   1620
acccttcctc tatataagga agttcatttc atttggagag aacaggatcc atgaaaaagc   1680
ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg   1740
acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc   1800
gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt   1860
atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggagttta   1920
gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc   1980
ctgaaaccga actgcccgct gttctacaac cggtcgcgga ggctatggat gcgatcgctg   2040
cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat   2100
acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa   2160
ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt   2220
gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg   2280
tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg   2340
attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc   2400
agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgcca cgactccggg   2460
cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg   2520
atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg   2580
tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag   2640
tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag aaaggcgccg   2700
agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc cctagatcta   2760
tggctcctaa gaagaagaga aaggttataa caatggtgag caagggcgag gagctgttca   2820
ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg   2880
tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca   2940
ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctgc   3000
agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc   3060
ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc   3120
gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg   3180
acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca   3240
acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc   3300
acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg   3360
gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca   3420
aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga   3480
tcactctcgg catggacgag ctgtacaagc cgcggttccc gggagacctt tagctagctt   3540
caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta   3600
tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt   3660
tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag   3720
aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac   3780
tagatcggaa attcgtaatc atggtcatag catgctggct tcgggttcat ccgggtaaaa   3840
attgaaccat aagggttact ggttgagggg gccggatcgt gccgggttta gtgtattttt   3900
aaattttttt tttagaattt tgtataacta ttgtaagtta tattaataca aagtattaac   3960
ataaaaaaca caaggaagat gggtaaaaaa ttgcaattat tgcaagtggt acatttattt   4020
cataatttaa agtttcaaac ttacaaattg aaaggtttac atttttaaca agtaaattta   4080
aaggttttgc attgcccttg taagttcgtc ataagcaata tgaactgatt gaccttcttc   4140
tggaatatta aattcggatg ggttaccatg tgttaatata tctccaagtt tctcgtcttc   4200
taggctatca acatcttcat gtccttgatt tcttcgtttc aatctaatcc aatctctgaa   4260
acatactaaa acttccaaag cattgcctgc caatgagtga tggatgtctc caagttgttg   4320
tcttgcttgg ttaaatgcac tctccgatgc aagcctagaa attgacacat tcagcacgtc   4380
ctgagccata gcgaaaagaa tagtaaattg ctttctattc tcatgccacc atcccaacag   4440
tgaaaattcc tttgtgcgag gttctttttg cttctgcaag taaaattgaa gttcatcaat   4500
gttcttgcta ctaatttgag tgttagaaaa tgtagaaaaa atattaatac tatcaagcat   4560
tcatcatcat ccacatggca tagtgggatt aatactgcct atagcacgag caaacatcat   4620
caattatatt tgcataataa ttatataatt attgtaaata atcatttagc ttgttcgtag   4680
aaacatataa atctggggtt tcagttggtc caatctccat ataagtacat aaagcattca   4740
ttaattggtg acaatcaaat atcttaatag aaggatttaa aacagcacca attacgtaaa   4800
tcggagaact tgaaaaaaaa tattttttaa attttgcttg catttttcaa caacatcctt   4860
atatttttct ttcttcttaa atttaaaaag tagaaaaaaa tttcagctat atgtattaaa   4920
accatagtaa caatatgata atatgctcca aaaaactcaa cagtagctgt ataaaattta   4980
tgtaaaaatt taacatcatt aatggcctcc caagctt                            5017


<210>  6

<211>  5149
<212>  DNA
<213>  artificial sequence

<220>  	
<223>  description of artificial sequence: synthetic DNA

<220>
<223>  Description of artificial sequence Donor sequence for ALS2 knock-in

<400>  6
gaattcgata ttggagagta aggaaggtaa actgaagttg gatttttctg cttggaggca     60
ggagttgatg gagcagaaag tgaagcaccc gttgaacttt aaaacttttg gtgatgctat    120
tcctccacaa tatgctatcc aggttctaga tgagttaact aatgggaatg ctattataag    180
tactggtgtg gggcaacacc agatgtgggc tgctcaatac tataagtaca gaaagccacg    240
ccaatggttg acatctggtg gattaggagc aatgggattt ggtttacccg ctgctattgg    300
tgcagctgtg ggaagaccgg atgaagttgt ggttgacatt gatggcgatg gcagtttcat    360
catgaatgtg caggagctgg caacaattaa ggtggagaat ctcccagtta agattatgtt    420
actgaataat caacacttgg gaatggtggt tcaactcgag gatcggttct ataaggctaa    480
cagagcacac acatacctgg ggaatccttc taatgaggcg gaaatctttc ctaatatgtt    540
gaaatttgca gaggcttgtg gtgtacctgc tgcaagggtg acacataggg atgatcttag    600
agctgccatt cagaagatgt tagacactcc tgggccatac ttgttggatg tgattgtacc    660
tcatcaggaa catgttctac ctatgattcc cagtggcgga gctttcaaag atgtgatcac    720
agagggtgac gggagaattt cctattgagt ttgagaagct gcagagctag ttctaggcgt    780
ctaggccttg tattatctaa aataaacttc tattaagcca aacatgttct gtctattagt    840
ttgttattag tttttgccgt ggctttgctc atcgtcactg ttgtactatt aagtagttga    900
tatttatgtt tgttttgcat catccccctt tggttttgaa tgtgaaggat ttcagcaaag    960
tttcatcctc tatttgcaac aatctggaga ttaatttcta atggagtagt ttagtgtaat   1020
aaagactagt caaagattca aatagaggac ctaacagaac tcgccgtaaa gactggcgaa   1080
cagttcatac agagtctctt acgactcaat gacaagaaga aaatcttcgt caacatggtg   1140
gagcacgaca cacttgtcta ctccaaaaat atcaaagata cagtctcaga agaccaaagg   1200
gcaattgaga cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca   1260
gctatctgtc actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat   1320
cattgcgata aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg tcccaaagat   1380
ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag   1440
caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct   1500
tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagagaac aggatccatg   1560
aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc   1620
gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta   1680
ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt   1740
tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg   1800
gagtttagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa   1860
gacctgcctg aaaccgaact gcccgctgtt ctacaaccgg tcgcggaggc tatggatgcg   1920
atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc   1980
ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac   2040
tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg   2100
atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc   2160
aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg   2220
ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt   2280
atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccacga   2340
ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc   2400
aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc   2460
gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt   2520
gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaagaaa   2580
ggcgccgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct   2640
agatctatgg ctcctaagaa gaagagaaag gttataacaa tggtgagcaa gggcgaggag   2700
ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag   2760
ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc   2820
atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttcggctac   2880
ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc   2940
gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac   3000
aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag   3060
ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac   3120
agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag   3180
atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc   3240
cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccgcc   3300
ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc   3360
gccgggatca ctctcggcat ggacgagctg tacaagccgc ggttcccggg agacctttag   3420
ctagcttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg   3480
atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc   3540
atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac   3600
gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct   3660
atgttactag atcggaaatt cgtaatcatg gtcatagcat gcttggaact tcctctttag   3720
gttggatgta atccctatta gggctttctc ttaattttat tattgaattg ttggctttta   3780
atctgagcaa gttgatttgc agctttctct cgagtcctag gagcaatacg ttatctctgt   3840
ctcctatttc ctagtggata atcttatgat ggaaatatgt ggagttagga aactgttgac   3900
tgctaaattt ctctttgtga ggcgtctgac aggtatgctt tcaatctata gcagtttgat   3960
cagactttgt ttacgtataa caatgttacg caaacaaaca cgtgcttttt aaacagttat   4020
aggtgcttag ctaccgacaa tacatcacat ataacaggta catgtatatc tggcgttttg   4080
cttttaaata gtacatttca tttttgtatt atgcactgac cagaccctgt ttatggggtt   4140
tgttgttgtg ttattcactg aatctttaac attcaatctt catgagaaac tattctttac   4200
ggcgtctaat gttctttcta ctaaacaacc aagtctttgt acctaacaca cattgtaatt   4260
gatcactaga aacttgtcaa gttgctgatt tagtaatcta ttttcttata atgaagatgg   4320
aacttatcat tcccaaaaat atatcctcct tttgttttca aggttacaaa ttctctagaa   4380
aatcatttca tgtggagtag ctagtatctt taaacattaa gtaattatct cctgagttct   4440
gcctgcctct tatatttctt tggtgattcc tcttttttta ggggtgccgt gctaggggat   4500
attttttgtg gagcaatcct tttgcggaac tacttatatt caatatatta agtattattg   4560
gtttatttct tttaaaatcc atatttgatt tcacaaccat aatcgggtaa ttcatgatac   4620
ccatgaatat ttctatcaaa ttcttaatgc ttctatataa gcacaattgt gattttactc   4680
gactttgagc atgtcttcaa agttgaaaat ttaggtgttt cttgcatggt gttatagctg   4740
tcaaagtggt gttagggatg aaaagttttg cggatgaggg agagctctgc atggcgtaga   4800
aggtcaccaa acatgtctcc tctctctatt tctactagca tcgcctagaa gcctatcaat   4860
ttgttgagag gacttatatt accgaggaag atacaaccgt ttttaaagtt aggaaaaaac   4920
attattcata agttatttac tatggttcta ggtgatcttg gtccatcata atcaagtttt   4980
catcttctta atttctctca tttttgcttt ggggtgtgtc ttagttttca tcacaaaggg   5040
aagaagatcc attagagcat cacatgttct ttgaacctaa gacaagactc tttatttaac   5100
ccccgacaca ttatccttca atgaagtttt ctcctaggga gagaagctt               5149


<210>  7

<211>  2468
<212>  DNA
<213>  Nicotiana Tabacum

<220>
<223>  ALS1 gene sequence (SurA)

<400>  7
ccactgggca aattagcgtg tattagatac actttggaaa ggttgagtgt gtaatgtgat     60
ttttgttcgc aaaaagtgtg taatagggat ttagtccata gtttaggggg taacttatgt    120
atttataggt taaatgatgg cgactaaata gagcgcccgt gcaattttta ctattaaagt    180
agtattaaat tttcatacga ccctatatct atggctggca ccaaatttct tcacatttgg    240
atccctcttt catttgttct catccatttt tgcgattcat gtgcatttaa tcagtaggac    300
ccctttttag cttagtagtg ctctcatgtt ctcaacttaa tattaaacca accacactcc    360
atctgcatta ccctccttcc agtttcgtct ctccctgccc tccccttcaa caatggcggc    420
ggcggctcca tctccctctt cttccgcttt ctccaaaacc ctatcgcctt cctcctccac    480
atcctccacc ctcctcccta gatcaacctt ccctttcccc caccaccccc acaagaccac    540
cccaccaccc ctccacctca cccacactca cattcacatt cacagccaac gccgtcgttt    600
caccatatcc aatgtcattt ccactaacca aaaagtttcc cagaccgaaa aaaccgaaac    660
tttcgtttcc cgttttgctc ctgacgaacc cagaaagggt tccgacgttc tcgtggaggc    720
tctcgaaaga gaaggggtta cggacgtctt tgcgtaccca ggtggcgctt ccatggagat    780
tcaccaagct ttgacccgtt caagcatcat ccgcaacgtg ctgccacgtc acgagcaggg    840
cggtgtcttc gccgctgagg gttacgcacg cgccaccgga tttcccggcg tttgcattgc    900
cacctctggc cccggcgcca ccaatctcgt cagcggcctc gctgacgcgc tactggatag    960
cgtccccatt gttgctataa caggtcaagt gccacgtagg atgataggta ctgatgcttt   1020
tcaggaaact cctattgttg aggtaactag atcgattacc aagcataatt atctcgttat   1080
ggacgtagag gatattccta gggttgtacg tgaagctttt ttcctcgcga gatcgggccg   1140
gcctggccct attttgattg atgtacctaa ggatattcag caacaattgg tgatacctga   1200
ctgggatcag ccaatgaggt tacctggtta catgtctagg ttacctaaat tgcccaatga   1260
gatgctttta gaacaaattg ttaggcttat ttctgagtca aagaagcctg ttttgtatgt   1320
ggggggtggg tgttcgcaat cgagtgagga cttgagacga ttcgtggagc tcacgggtat   1380
ccccgtggca agtactttga tgggtcttgg agcttttcca actggggatg agctttccct   1440
ttcaatgttg ggtatgcatg gtactgttta tgctaattat gctgtggaca gtagtgattt   1500
gttgctcgca tttggggtga ggtttgatga tagagttact ggaaagttag aagcttttgc   1560
tagccgagca aaaattgttc acattgatat tgattcagct gagattggaa agaacaagca   1620
gcctcatgtt tccatttgtg cagatatcaa gttggcgtta cagggtttga attcgatact   1680
ggagagtaag gaaggtaaac tgaagttgga tttttctgct tggaggcagg agttgacgga   1740
gcagaaagtg aagcacccat tgaactttaa aacttttggt gatgcaattc ctccgcaata   1800
tgctatccag gttctagatg agttaactaa tgggaatgct attataagta ctggtgtggg   1860
gcaacaccag atgtgggctg ctcaatacta taagtacaga aagccacgcc aatggttgac   1920
atctggtgga ttaggagcaa tgggatttgg tttgcccgct gctattggtg cggctgttgg   1980
aagaccggat gaagttgtgg ttgacattga tggtgatggc agtttcatca tgaatgtgca   2040
ggagcttgca acaattaagg tggagaatct cccagttaag attatgttac tgaataatca   2100
acacttggga atggtggttc aatgggagga tcggttctat aaggctaaca gagcacacac   2160
atacctgggg aatccttcta atgaggcgga gatctttcct aatatgctga aatttgcaga   2220
ggcttgtggc gtacctgctg caagagtgac acatagggat gatcttagag ctgccattca   2280
gaagatgtta gacactcctg ggccatactt gttggatgtg attgtacctc atcaggaaca   2340
tgttttacct atgattccca gtggcggagc tttcaaagat gtgatcacag agggtgacgg   2400
gagaagttcc tattgagttt gagaagctac agagctagat tctaggcctt gtattatcta   2460
aaataaac                                                            2468


<210>  8

<211>  2461
<212>  DNA
<213>  Nicotiana Tabacum

<220>
<223>  ALS2 gene sequence (SurB)

<400>  8
tatttcttag cttgtttttt ttttgttcta tattgttact ttgagctata tttcataaca     60
gcattcacat tctttttcca tagtcttttt tcccttttat attttaattt actgaagtaa    120
caaatacttc cacttctttc ttcttcccac cctcctaaat atatccaaca tctcattttt    180
cttttcccca attctcagac attttaatct ttcttttcta tttattttct tcatattttg    240
atctctcttc catttgttct catccatttt cgctattcac gtgaattcaa tcaagtagga    300
ccctttcagt ttcgtggcgc tctcgtcttc tcagcttaat ataaaaccaa ccacacacca    360
tctacattgc cctttccttt cagtttcgtc tctcactgct ctcattcaac aataatggcg    420
gcggctgcgg cggctccatc tccctctttc tccaaaaccc tatcgtcctc ctcctccaaa    480
tcctccaccc tcctccctag atccaccttc cctttccccc accaccccca caaaaccacc    540
ccaccacccc tccacctcac ccccacccac attcacagcc aacgccgtcg tttcaccatc    600
tccaatgtca tttccactac ccaaaaagtt tccgagaccc aaaaagccga aactttcgtt    660
tcccgttttg cccctgacga acccagaaag ggttccgacg ttctcgtgga ggccctcgaa    720
agagaagggg ttacggacgt ttttgcgtac ccaggcggcg cttccatgga gattcaccaa    780
gctttgacgc gctcaagcat catccgcaac gtgctaccac gtcacgagca gggtggtgtc    840
ttcgccgctg agggttacgc acgcgccacc ggcttccccg gcgtttgcat tgccacctcc    900
ggccctggcg ccaccaatct cgtcagtggc ctcgcggacg ccctactgga tagcgtcccc    960
attgttgcta taaccggtca agtgccacgt aggatgatcg gtactgatgc ttttcaggaa   1020
actccgattg ttgaggtaac tagatcgatt accaagcata attatctcgt tatggacgta   1080
gaggatattc ctagggttgt acgtgaggct tttttccttg cgagatcggg ccggcctggc   1140
cctgttttga ttgatgtacc taaggatatt cagcaacaat tggtgatacc tgactgggat   1200
cagccaatga ggttgcctgg ttacatgtct aggttaccta aattgcccaa tgagatgctt   1260
ttagaacaaa ttgttaggct tatttctgag tcaaagaagc ctgttttgta tgtggggggt   1320
gggtgttcgc aatcgagtga ggagttgaga cgattcgtgg agctcaccgg tatccccgtg   1380
gcaagtactt tgatgggtct tggagctttt ccaactgggg atgagctttc cctttcaatg   1440
ttgggtatgc atggtactgt ttatgctaat tatgctgtgg acagtagtga tttattgctc   1500
gcatttgggg tgaggtttga tgatagagtt actggaaagt tagaagcttt tgctagccga   1560
gcgaaaattg ttcacattga tattgattca gctgagattg gaaagaacaa gcagcctcat   1620
gtttccattt gtgcggatat caagttggcg ttacagggtt tgaattcgat attggagagt   1680
aaggaaggta aactgaagtt ggatttttct gcttggaggc aggagttgac ggtgcagaaa   1740
gtgaagtacc cgttgaattt taaaactttt ggtgatgcta ttcctccgca atatgctatc   1800
caggttctag atgagttaac taatgggagt gctattataa gtaccggtgt tgggcagcac   1860
cagatgtggg ctgctcaata ttataagtac agaaagccac gccaatggtt gacatctggt   1920
ggattaggag cgatgggatt tggtttgccc gctgctattg gtgcggctgt tggaagacct   1980
gatgaagttg tggttgacat tgatggtgat ggcagtttca tcatgaatgt gcaggagcta   2040
gcaactatta aggtggagaa tctcccagtt aagattatgt tactgaataa tcaacacttg   2100
ggaatggtgg ttcaatggga ggatcggttc tataaggcta acagagcaca cacatacctg   2160
gggaatcctt ctaatgaggc ggagatcttt cctaatatgt tgaaatttgc agaggcttgt   2220
ggcgtacctg ctgcgagagt gacacacagg gatgatctta gagcggctat tcaaaagatg   2280
ttagacactc ctgggccata cttgttggat gtgattgtac ctcatcagga acatgttcta   2340
cctatgattc ccagtggcgg ggctttcaaa gatgtgatca cagagggtga cgggagaagt   2400
tcctattgac tttgaggtgc tacagagcta gattctaggc cttgtattat ctaaaataaa   2460
c                                                                   2461


<210>  9

<211>  2122
<212>  DNA
<213>  Solanum tuberosum

<220>
<223>  Solanum tuberosum AHAS gene

<400>  9
tagccatttt gcctcctttc acttctcacc tttatcgaca acaccaacat ggcggctgct     60
gcctcaccat ctccatgttt ctccaaaacc ctacctccat cttcctccaa atcttccacc    120
attcttccta gatctacctt ccctttccac aatcaccctc aaaaagcctc accccttcat    180
ctcacccaca cccatcatca tcgtcgtggt ttcgccgttt ccaatgtcgt catatccact    240
accacccata acgacgtttc tgaacctgaa acattcgttt cccgtttcgc ccctgacgaa    300
cccagaaagg gttgtgatgt tcttgtggag gcacttgaaa gggagggggt tacggatgta    360
tttgcgtacc caggaggtgc ttctatggag attcatcagg ctttgacacg ttcgaatatt    420
attcgtaatg tgctgccacg tcatgagcaa ggtggtgtgt ttgctgcaga gggttacgca    480
cgggcgactg ggttccctgg tgtttgcatt gctacctctg gtccgggagc tacgaatctt    540
gttagtggtc ttgcggatgc tttgttggat agtattccga ttgttgctat tacgggtcaa    600
gtgccgagga ggatgattgg tactgatgcg tttcaggaaa cgcctattgt tgaggtaacg    660
agatctatta cgaagcataa ttatcttgtt atggatgtag aggatattcc tagggttgtt    720
cgtgaagcgt tttttctagc gaaatcggga cggcctgggc cggttttgat tgatgtacct    780
aaggatattc agcaacaatt ggtgatacct aattgggatc agccaatgag gttgcctggt    840
tacatgtcta ggttacctaa attgcctaat gagatgcttt tggaacaaat tattaggctg    900
atttcggagt cgaagaagcc tgttttgtat gtgggtggtg ggtgtttgca atcaagtgag    960
gagctgagac gatttgtgga gcttacgggt attcctgtgg cgagtacttt gatgggtctt   1020
ggagcttttc caactgggga tgagctttcc cttcaaatgt tgggtatgca tgggactgtg   1080
tatgctaatt atgctgtgga tggtagtgat ttgttgcttg catttggggt gaggtttgat   1140
gatcgagtta ctggtaaatt ggaagctttt gctagccgag cgaaaattgt ccacattgat   1200
attgattcgg ctgagattgg aaagaacaag caacctcatg tttccatttg tgcagatatc   1260
aagttggcat tacagggttt gaattccata ttggagggta aagaaggtaa gctgaagttg   1320
gacttttctg cttggagaca ggagttaacg gaacagaagg tgaagtaccc attgagtttt   1380
aagacttttg gtgaagccat ccctccacaa tatgctattc aggttcttga tgagttaact   1440
aacggaaatg ccattattag tactggtgtg gggcaacacc agatgtgggc tgcccaatac   1500
tataagtaca aaaagccaca ccaatggttg acatctggtg gattaggagc aatgggattt   1560
ggtttgcctg ctgcaatagg tgcggctgtt ggaagaccgg gtgagattgt ggttgacatt   1620
gatggtgacg ggagttttat catgaatgtg caggagttag caacaattaa ggtggagaat   1680
ctcccagtta agattatgtt gctgaataat caacacttgg gaatggtggt tcaatgggag   1740
gatcgattct ataaggctaa cagagcacac acttacttgg gtgatcctgc taatgaggaa   1800
gagatcttcc ctaatatgtt gaaattcgca gaggcttgtg gcgtacctgc tgcaagagtg   1860
tcacacaggg atgatcttag agctgccatt caaaagatgt tagacactcc tgggccatac   1920
ttgttggatg tgattgtacc tcatcaggag cacgttctac ctatgattcc cagtggcggt   1980
gctttcaaag atgtgatcac agagggtgat gggagacgtt catattgact tttagaaact   2040
acataactag ctctaggcat tgtattatct aaaataaact tctattaagc caaaagtgtt   2100
ctatctgtct agtttgccgt tg                                            2122


<210>  10

<211>  1962
<212>  DNA
<213>  Capsicum Annum

<220>
<223>  Capsicum Annum AHAS gene

<220>
<221>  misc_feature
<222>  (1935)
<223>  w is a or t

<400>  10
atggcggctg cagcgccccc tcctcctcct ttcaccaaaa ccctatctca ttccccttcc     60
tcctccgcca aatcccccac tcttctccct agatccacct tcccttttac ccggcccctt    120
ctccttatcc accgccgtcg tttcacggtc aacaatgtca tttccactaa tcataacgtt    180
tccctttctc aaccacccga aacatttatt acccgttttg cccctgacga gccccgaaaa    240
ggctgcgacg ttctcgtgga agcactcgaa agagaagggg ttaccgacgt ctttgcatac    300
cctggcggtg cttcagtgga gattcatcag gcattgactc gttcgaatat aatccggaat    360
gtcctgccac gtcatgagca aggtggcgta ttcgctgccg agggttacgc gcgcgccacc    420
gggttccctg gtgtgtgcat tgcgacatca gggccgggag ctacgaatct cgttagcggg    480
cttgcggatg cgttgttgga tagtattccg atagtggcta ttacgggtca ggtgccgagg    540
aggatgattg gtacggatgc gtttcaggag actccgattg tcgaggtaac taggtctata    600
acgaagcata attatcttgt tatggatgta gaggatattc ccagggttgt tcgtgaggca    660
tttttcctag caaaatcggg gaggcctggt ccagttttga ttgatgttcc taaggatatt    720
cagcagcaat tggtgatacc taattgggat cagccaatga ggttgcctgg ttacatgtct    780
aggttgccta aattgcctaa tgagatgctt ttggaacaaa ttgttaggct tatttctgag    840
tcaaagaagc ctgttttgta tgtgggaggt gggtgttcgc agtcgagtga ggagttgaga    900
cgatttgtgg agcttactgg tattcccgtt gcgactactt tgatgggtct tggagctttt    960
ccaactgggg atgacctttc tcttcagatg ttgggtatgc atggcactgt ctatgctaat   1020
tatgctgtcg atagtagtga tttgctgctt gcgtttgggg tgaggtttga tgatagggtt   1080
actggtaaat tggaagcttt tgctagccgt gcgaaaattg tccacattga cattgattca   1140
gctgagattg gaaagaacaa gcagcctcat gtttcaattt gtgcagatat caagttggca   1200
ttacagggtt tgaattccat attggagggt aaagaagcta agctgaagaa gttggacttt   1260
tctgcttgga ggcaggagtt aaacgagcag aaagtgaagt acccattgaa ttataagact   1320
tttggtgatg ccatccctcc acaatatgct attcaggttc tagatgagtt aaccgacgga   1380
aatgccattg ttagtactgg tgtggggcaa caccagatgt gggctgccca gtactataag   1440
ttcaaaaagc cacgccaatg gttgacatct ggtggattag gagcaatggg atttggtttg   1500
cccgctgcta taggtgcggc tgttggaaga cccggggaga ttgtggttga cattgatggt   1560
gatgggagtt ttatcatgaa tgtgcaggag ttagcaacaa ttaaggtgga gaacctccca   1620
gttaagatta tgttgctgaa taatcaacac ttgggaatgg tggttcaatg ggaggatcgg   1680
ttctacaagg ctaacagagc acacacttac ctgggtaatc ctgcaaatga ggaagaaatc   1740
tttcctaata tgttgaaatt tgcagaggct tgtggcgtac ctgctgcaag agtgacacac   1800
agggatgatg ttagagctgc cattcagaag atgttggaca ctcctggacc atacttgttg   1860
gatgtgattg taccgcatca ggagcacgtt ttacctatga ttcccagtgg tggtgctttc   1920
aaagatgtga ttacwgaggg tgatgggaga tgttcccact ga                      1962

<210>  11

<211>  4048
<212>  DNA
<213>  artificial sequence

<220>  	
<223>  description of artificial sequence: synthetic DNA

<220>
<223>  Donor sequence for ALS2 Knock-in

<400>  11
gatattggag agtaaggaag gtaaactgaa gttggatttt tctgcttgga ggcaggagtt     60
gatggagcag aaagtgaagc acccgttgaa ctttaaaact tttggtgatg ctattcctcc    120
acaatatgct atccaggttc tagatgagtt aactaatggg aatgctatta taagtactgg    180
tgtggggcaa caccagatgt gggctgctca atactataag tacagaaagc cacgccaatg    240
gttgacatct ggtggattag gagcaatggg atttggttta cccgctgcta ttggtgcagc    300
tgtgggaaga ccggatgaag ttgtggttga cattgatggc gatggcagtt tcatcatgaa    360
tgtgcaggag ctggcaacaa ttaaggtgga gaatctccca gttaagatta tgttactgaa    420
taatcaacac ttgggaatgg tggttcaact cgaggatcgg ttctataagg ctaacagagc    480
acacacatac ctggggaatc cttctaatga ggcggaaatc tttcctaata tgttgaaatt    540
tgcagaggct tgtggtgtac ctgctgcaag ggtgacacat agggatgatc ttagagctgc    600
cattcagaag atgttagaca ctcctgggcc atacttgttg gatgtgattg tacctcatca    660
ggaacatgtt ctacctatga ttcccagtgg cggagctttc aaagatgtga tcacagaggg    720
tgacgggaga atttcctatt gagtttgaga agctgcagag ctagttctag gcgtctaggc    780
cttgtattat ctaaaataaa cttctattaa gccaaacatg ttctgtctat tagtttgtta    840
ttagtttttg ccgtggcttt gctcatcgtc actgttgtac tattaagtag ttgatattta    900
tgtttgtttt gcatcatccc cctttggttt tgaatgtgaa ggatttcagc aaagtttcat    960
cctctatttg caacaatctg gagattaatt tctaatggag tagtttagtg taataaagac   1020
tagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg cgaacagttc   1080
atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat ggtggagcac   1140
gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca aagggcaatt   1200
gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg cccagctatc   1260
tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg ccatcattgc   1320
gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa agatggaccc   1380
ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg   1440
gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta tccttcgcaa   1500
gacccttcct ctatataagg aagttcattt catttggaga gaacaggatc tatggctcct   1560
aagaagaaga gaaaggttat aacaatggtg agcaagggcg aggagctgtt caccggggtg   1620
gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc   1680
gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc   1740
aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcct gcagtgcttc   1800
gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc   1860
tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag   1920
gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag   1980
gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat   2040
atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc   2100
gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc   2160
cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc   2220
aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc   2280
ggcatggacg agctgtacaa gccgcggttc ccgggagacc tttagctagc ttcaaacatt   2340
tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa   2400
tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg   2460
agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa   2520
atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg   2580
aaattcgtaa tcatggtcat agcatgcttg gaacttcctc tttaggttgg atgtaatccc   2640
tattagggct ttctcttaat tttattattg aattgttggc ttttaatctg agcaagttga   2700
tttgcagctt tctctcgagt cctaggagca atacgttatc tctgtctcct atttcctagt   2760
ggataatctt atgatggaaa tatgtggagt taggaaactg ttgactgcta aatttctctt   2820
tgtgaggcgt ctgacaggta tgctttcaat ctatagcagt ttgatcagac tttgtttacg   2880
tataacaatg ttacgcaaac aaacacgtgc tttttaaaca gttataggtg cttagctacc   2940
gacaatacat cacatataac aggtacatgt atatctggcg ttttgctttt aaatagtaca   3000
tttcattttt gtattatgca ctgaccagac cctgtttatg gggtttgttg ttgtgttatt   3060
cactgaatct ttaacattca atcttcatga gaaactattc tttacggcgt ctaatgttct   3120
ttctactaaa caaccaagtc tttgtaccta acacacattg taattgatca ctagaaactt   3180
gtcaagttgc tgatttagta atctattttc ttataatgaa gatggaactt atcattccca   3240
aaaatatatc ctccttttgt tttcaaggtt acaaattctc tagaaaatca tttcatgtgg   3300
agtagctagt atctttaaac attaagtaat tatctcctga gttctgcctg cctcttatat   3360
ttctttggtg attcctcttt ttttaggggt gccgtgctag gggatatttt ttgtggagca   3420
atccttttgc ggaactactt atattcaata tattaagtat tattggttta tttcttttaa   3480
aatccatatt tgatttcaca accataatcg ggtaattcat gatacccatg aatatttcta   3540
tcaaattctt aatgcttcta tataagcaca attgtgattt tactcgactt tgagcatgtc   3600
ttcaaagttg aaaatttagg tgtttcttgc atggtgttat agctgtcaaa gtggtgttag   3660
ggatgaaaag ttttgcggat gagggagagc tctgcatggc gtagaaggtc accaaacatg   3720
tctcctctct ctatttctac tagcatcgcc tagaagccta tcaatttgtt gagaggactt   3780
atattaccga ggaagataca accgttttta aagttaggaa aaaacattat tcataagtta   3840
tttactatgg ttctaggtga tcttggtcca tcataatcaa gttttcatct tcttaatttc   3900
tctcattttt gctttggggt gtgtcttagt tttcatcaca aagggaagaa gatccattag   3960
agcatcacat gttctttgaa cctaagacaa gactctttat ttaacccccg acacattatc   4020
cttcaatgaa gttttctcct agggagag                                      4048


<210>  12

<211>  29
<212>  DNA
<213>  artificial sequence

<220>  	
<223>  description of artificial sequence: synthetic oligonucleotide

<220>
<223>  Forward primer tobacco ALS for target insertion genotyping

<400>  12
atttccacta tccaaaaagt ttccgagac                                       29


<210>  13

<211>  29
<212>  DNA
<213>  artificial sequence

<220>  	
<223>  description of artificial sequence: synthetic oligonucleotide

<220>
<223>  Reverse primer tobacco ALS for target insertion genotyping

<400>  13
cgcgtattaa atgtataatt gcgggactc                                       29




