                                SEQUENCE LISTING

<110> Chronix Biomedical

<120> Personalized Biomarkers for Cancer
  

<130> 83443-893907

<140> WO PCT/US13/75015   
<141> 2013-12-13  

<150> US 61/737,626       
<151> 2012-12-14  

<160> 33

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 58
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic P9 adapter oligonucleotide, P9 oligo

<220> 
<221> modified_base  
<222> (1)...(1)
<223> g modified by 5' phosphate group

<400> 1
gatcggaaga gcatcgcaag gcaagcaagc taaaactgaa ccgggttcct cattagcg   58

<210> 2
<211> 58
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic P5 adapter oligonucleotide, P5 oligo

<220> 
<221> modified_base  
<222> (57)...(58)
<223> phosphorothioate internucleotide linkage

<400> 2
aatgatacgg cgaccaccga gatctacact ctttccctac acgacgctct tccgatct   58

<210> 3
<211> 58
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic PCR amplification primer P9-PCR-Primer

<400> 3
cgctaatgag gaacccggtt cagttttagc ttgcttgcct tgcgatgctc ttccgatc   58

<210> 4
<211> 58
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic PCR amplification primer P5-Universal,
      Alu-enrichment PCR primer P5-Universal

<400> 4
aatgatacgg cgaccaccga gatctacact ctttccctac acgacgctct tccgatct   58

<210> 5
<211> 56
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic Alu-enrichment PCR primer Alu40.REV

<400> 5
gtgactggag ttcagacgtg tgctcttccg atctcccaaa gtgctgggat tacagg     56

<210> 6
<211> 58
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic Alu-enrichment PCR primer Alu259.FOR

<400> 6
gtgactggag ttcagacgtg tgctcttccg atcggcraca gagcgaracy cygtctca   58

<210> 7
<211> 64
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic index-primer, fusion primer with 7 bp
      index sequence

<221> modified_base  
<222> (25)...(31)
<223> n = g, a, c or t, 7 bp index sequence, molecular barcode

<400> 7
caagcagaag acggcatacg agatnnnnnn ngtgactgga gttcagacgt gtgctcttcc 60
gatc                                                              64

<210> 8

<400> 8

000

<210> 9
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic biotinylated L1-specific oligonucleotide
      probe Probe4000

<400> 9
ctggcacaag acagggatgc cctctctcac cgctcctatt caacatagtg ttggaagttc 60


<210> 10
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic biotinylated L1-specific oligonucleotide
      probe Probe4117

<400> 10
ccccatcgtc tcagcccaaa atctccttaa gctgataagc aacttcagca aagtctcagg 60


<210> 11
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic biotinylated L1-specific oligonucleotide
      probe Probe4500

<400> 11
ctggaggcat cacactacct gacttcaaac tatactacaa ggctacagta accaaaacag 60


<210> 12
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic biotinylated L1-specific oligonucleotide
      probe Probe4759

<400> 12
gcaatgggga aaggattccc tatttaataa atggtgctgg gaaaactggc tagccatatg 60


<210> 13
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic biotinylated L1-specific oligonucleotide
      probe Probe5000

<400> 13
ggcgtgggca aggacttcat gtccaaaaca ccaaaagcaa tggcaacaaa agccaaaatt 60


<210> 14
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic biotinylated L1-specific oligonucleotide
      probe Probe5182

<400> 14
aaacaacccc atcaaaaagt gggcgaagga catgaacaga cacttctcaa aagaagacat 60


<210> 15
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic biotinylated L1-specific oligonucleotide
      probe Probe5500

<400> 15
cactgttggt gggactgtaa actagttcaa ccattgtgga agtcagtgtg gcgattcctc 60


<210> 16
<211> 61
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic biotinylated L1-specific oligonucleotide
      probe Probe5699

<400> 16
gatgagttca tatcctttgt agggacatgg atgaaattgg aaaccatcat tctcagtaaa 60
c                                                                 61

<210> 17
<211> 60
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic biotinylated L1-specific oligonucleotide
      probe Probe6000

<400> 17
gggtgcagcg caccagcatg gcacatgtat acatatgtaa ctaacctgca caatgtgcac 60


<210> 18
<211> 26
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic PCR primer 16-chr17:27923338-27923393.F

<400> 18
ttgtattact tcttgaccac attaaa                                      26

<210> 19
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic PCR primer 16-chr17:27923338-27923393.R

<400> 19
cgagggggag taaaagtgct                                             20

<210> 20
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic PCR primer 16-chr14:38578285-38578387.F

<400> 20
aaatgcacct cttctctcca                                             20

<210> 21
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic PCR primer 16-chr14:38578285-38578387.R

<400> 21
atgctgagtt ttggactcaa                                             20

<210> 22
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic PCR primer 16-chr3:74888046-74888097.F

<400> 22
aaacatcttt aatccccagc a                                           21

<210> 23
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic PCR primer 16-chr3:74888046-74888097.R

<400> 23
ttccttagat caggcacatt ttt                                         23

<210> 24
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic PCR primer 5-chr2:159718375-159718655.F

<400> 24
gccacaggtc tttgaagagg                                             20

<210> 25
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic PCR primer 5-chr2:159718375-159718655.R

<400> 25
ggaacctgca caccctgtat                                             20

<210> 26
<211> 26
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic PCR primer 10-chr13:90100261-90100403.F

<400> 26
caaatctcaa gagtttatga tgtacc                                      26

<210> 27
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic PCR primer 10-chr13:90100261-90100403.R

<400> 27
cctagagaga ttgggggcta a                                           21

<210> 28
<211> 22
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic PCR primer 10-chr2:196460079-196460192.F

<400> 28
gcacatttta aaaagcttgc ag                                          22

<210> 29
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic PCR primer 10-chr2:196460079-196460192.R

<400> 29
cgaattttct atgtaactga aaca                                        24

<210> 30
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic PCR primer 10-chr10:131942955-131943134.F

<400> 30
gagagggctt cctgagatac c                                           21

<210> 31
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic PCR primer 10-chr10:131942955-131943134.R

<400> 31
tccaccatcg gagaataagg                                             20

<210> 32
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic PCR primer 12-chr22:33566363-33566494.F

<400> 32
agccacatga ccgccttct                                              19

<210> 33
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic PCR primer 12-chr22:33566363-33566494.R

<400> 33
tgtttgacca ggaccctgac                                             20


