               SEQUENCE LISTING

<110> Thrombotargets Europe SL

<120> Sealant composition

<130> P2530EP00

<150> EP12198839.8
<151> 2012-12-21

<160> 11

<170> BiSSAP 1.0

<210> 1
<211> 263
<212> PRT
<213> Homo sapiens

<220> 
<221> SOURCE
<222> 1..263
<223> /mol_type="protein"
      /organism="Homo sapiens"

<400> 1
Ser Gly Thr Thr Asn Thr Val Ala Ala Tyr Asn Leu Thr Trp Lys Ser 
1               5                   10                   15    
Thr Asn Phe Lys Thr Ile Leu Glu Trp Glu Pro Lys Pro Val Asn Gln 
            20                   25                  30        
Val Tyr Thr Val Gln Ile Ser Thr Lys Ser Gly Asp Trp Lys Ser Lys 
        35                   40                  45            
Cys Phe Tyr Thr Thr Asp Thr Glu Cys Asp Leu Thr Asp Glu Ile Val 
    50                   55                  60                
Lys Asp Val Lys Gln Thr Tyr Leu Ala Arg Val Phe Ser Tyr Pro Ala 
65                   70                  75                  80
Gly Asn Val Glu Ser Thr Gly Ser Ala Gly Glu Pro Leu Tyr Glu Asn 
                85                   90                  95    
Ser Pro Glu Phe Thr Pro Tyr Leu Glu Thr Asn Leu Gly Gln Pro Thr 
            100                  105                110        
Ile Gln Ser Phe Glu Gln Val Gly Thr Lys Val Asn Val Thr Val Glu 
        115                  120                125            
Asp Glu Arg Thr Leu Val Arg Arg Asn Asn Thr Phe Leu Ser Leu Arg 
    130                  135                140                
Asp Val Phe Gly Lys Asp Leu Ile Tyr Thr Leu Tyr Tyr Trp Lys Ser 
145                  150                155                  160
Ser Ser Ser Gly Lys Lys Thr Ala Lys Thr Asn Thr Asn Glu Phe Leu 
                165                  170                175    
Ile Asp Val Asp Lys Gly Glu Asn Tyr Cys Phe Ser Val Gln Ala Val 
            180                  185                190        
Ile Pro Ser Arg Thr Val Asn Arg Lys Ser Thr Asp Ser Pro Val Glu 
        195                  200                205            
Cys Met Gly Gln Glu Lys Gly Glu Phe Arg Glu Ile Phe Tyr Ile Ile 
    210                  215                220                
Gly Ala Val Val Phe Val Val Ile Ile Leu Val Ile Ile Leu Ala Ile 
225                  230                235                  240
Ser Leu His Lys Cys Arg Lys Ala Gly Val Gly Gln Ser Trp Lys Glu 
                245                  250                255    
Asn Ser Pro Leu Asn Val Ser 
            260            

<210> 2
<211> 6
<212> PRT
<213> artificial sequences

<220> 
<221> SOURCE
<222> 1..6
<223> /mol_type="protein"
      /note="epitope AHGHRP"
      /organism="artificial sequences"

<400> 2
Ala His Gly His Arg Pro 
1               5    

<210> 3
<211> 14
<212> PRT
<213> artificial sequences

<220> 
<221> SOURCE
<222> 1..14
<223> /mol_type="protein"
      /note="Epitope"
      /organism="artificial sequences"

<400> 3
Pro Ile His Asp His Asp His Pro His Leu Val Ile His Ser 
1               5                   10                

<210> 4
<211> 7
<212> PRT
<213> artificial sequences

<220> 
<221> SOURCE
<222> 1..7
<223> /mol_type="protein"
      /note="X can be any naturally occurring amino acid"
      /organism="artificial sequences"

<400> 4
Gly Met Thr Cys Xaa Xaa Cys 
1               5        

<210> 5
<211> 269
<212> PRT
<213> artificial sequences

<220> 
<221> SOURCE
<222> 1..269
<223> /mol_type="protein"
      /note="Human mature TF with N124A mutation and C terminal hexahis
      tidine "
      /organism="artificial sequences"

<400> 5
Ser Gly Thr Thr Asn Thr Val Ala Ala Tyr Asn Leu Thr Trp Lys Ser 
1               5                   10                   15    
Thr Asn Phe Lys Thr Ile Leu Glu Trp Glu Pro Lys Pro Val Asn Gln 
            20                   25                  30        
Val Tyr Thr Val Gln Ile Ser Thr Lys Ser Gly Asp Trp Lys Ser Lys 
        35                   40                  45            
Cys Phe Tyr Thr Thr Asp Thr Glu Cys Asp Leu Thr Asp Glu Ile Val 
    50                   55                  60                
Lys Asp Val Lys Gln Thr Tyr Leu Ala Arg Val Phe Ser Tyr Pro Ala 
65                   70                  75                  80
Gly Asn Val Glu Ser Thr Gly Ser Ala Gly Glu Pro Leu Tyr Glu Asn 
                85                   90                  95    
Ser Pro Glu Phe Thr Pro Tyr Leu Glu Thr Asn Leu Gly Gln Pro Thr 
            100                  105                110        
Ile Gln Ser Phe Glu Gln Val Gly Thr Lys Val Ala Val Thr Val Glu 
        115                  120                125            
Asp Glu Arg Thr Leu Val Arg Arg Asn Asn Thr Phe Leu Ser Leu Arg 
    130                  135                140                
Asp Val Phe Gly Lys Asp Leu Ile Tyr Thr Leu Tyr Tyr Trp Lys Ser 
145                  150                155                  160
Ser Ser Ser Gly Lys Lys Thr Ala Lys Thr Asn Thr Asn Glu Phe Leu 
                165                  170                175    
Ile Asp Val Asp Lys Gly Glu Asn Tyr Cys Phe Ser Val Gln Ala Val 
            180                  185                190        
Ile Pro Ser Arg Thr Val Asn Arg Lys Ser Thr Asp Ser Pro Val Glu 
        195                  200                205            
Cys Met Gly Gln Glu Lys Gly Glu Phe Arg Glu Ile Phe Tyr Ile Ile 
    210                  215                220                
Gly Ala Val Val Phe Val Val Ile Ile Leu Val Ile Ile Leu Ala Ile 
225                  230                235                  240
Ser Leu His Lys Cys Arg Lys Ala Gly Val Gly Gln Ser Trp Lys Glu 
                245                  250                255    
Asn Ser Pro Leu Asn Val Ser His His His His His His 
            260                  265                

<210> 6
<211> 813
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..813
<223> /mol_type="DNA"
      /note="DNA encoding human mature TF with N124A and C-terminal "
      /organism="artificial sequences"

<400> 6
atgtcaggca ctacaaatac tgtggcagca tataatttaa cttggaaatc aactaatttc      60

aagacaattt tggagtggga acccaaaccc gtcaatcaag tctacactgt tcaaataagc     120

actaagtcag gagattggaa aagcaaatgc ttttacacaa cagacacaga gtgtgacctc     180

accgacgaga ttgtgaagga tgtgaagcag acgtacttgg cacgggtctt ctcctacccg     240

gcagggaatg tggagagcac cggttctgct ggggagcctc tgtatgagaa ctccccagag     300

ttcacacctt acctggagac aaacctcgga cagccaacaa ttcagagttt tgaacaggtg     360

ggaacaaaag tggcagtgac cgtagaagat gaacggactt tagtcagaag gaacaacact     420

ttcctaagcc tccgggatgt ttttggcaag gacttaattt atacacttta ttattggaaa     480

tcttcaagtt caggaaagaa aacagccaaa acaaacacta atgagttttt gattgatgtg     540

gataaaggag aaaactactg tttcagtgtt caagcagtga ttccctcccg aacagttaac     600

cggaagagta cagacagccc ggtagagtgt atgggccagg agaaagggga attcagagaa     660

atattctaca tcattggagc tgtggtattt gtggtcatca tccttgtcat catcctggct     720

atatctctac acaagtgtag aaaggcagga gtggggcaga gctggaagga gaactcccca     780

ctgaatgttt cacatcacca tcaccatcac tag                                  813


<210> 7
<211> 33
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..33
<223> /mol_type="DNA"
      /note="upstream primer encoding the first amino acids of mature T
      F lacking the signal peptide and containing an initiation codon A
      TG  in frame with TF ORF), and a restriction BamHI site"
      /organism="artificial sequences"

<400> 7
gggatccgca tgtcaggcac tacaaatact gtg                                  33


<210> 8
<211> 50
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..50
<223> /mol_type="DNA"
      /note="downstream primer containing the histidine coding nucleoti
      des  and a new termination signal TAG, both containing a restrict
      ion BamHI site"
      /organism="artificial sequences"

<400> 8
cggatccgtg tcgacctagt gatggtgatg gtgatgtgaa acattcagtg                50


<210> 9
<211> 33
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..33
<223> /mol_type="DNA"
      /note="primer"
      /organism="artificial sequences"

<400> 9
gtgggaacaa aagtggcagt gaccgtagaa gat                                  33


<210> 10
<211> 27
<212> DNA
<213> artificial sequences

<220> 
<221> source
<222> 1..27
<223> /mol_type="DNA"
      /note="primer"
      /organism="artificial sequences"

<400> 10
atcttctacg gtcactgcca cttttgt                                        27


<210> 11
<211> 1374
<212> DNA
<213> Homo sapiens

<220> 
<221> source
<222> 1..1374
<223> /mol_type="DNA"
      /organism="Homo sapiens"

<400> 11
gggggcgccg ccggcccttt atagcgcgcg gggcaccggc tccccaagac tgcgagctcc      60

ccgcaccccc tcgcactccc tctggccggc ccagggcgcc ttcagcccaa cctccccagc     120

cccacgggcg ccacggaacc cgctcgatct cgccgccaac tggtagacat ggagacccct     180

gcctggcccc gggtcccgcg ccccgagacc gccgtcgctc ggacgctcct gctcggctgg     240

gtcttcgccc aggtggccgg cgcttcaggc actacaaata ctgtggcagc atataattta     300

acttggaaat caactaattt caagacaatt ttggagtggg aacccaaacc cgtcaatcaa     360

gtctacactg ttcaaataag cactaagtca ggagattgga aaagcaaatg cttttacaca     420

acagacacag agtgtgacct caccgacgag attgtgaagg atgtgaagca gacgtacttg     480

gcacgggtct tctcctaccc ggcagggaat gtggagagca ccggttctgc tggggagcct     540

ctgtatgaga actccccaga gttcacacct tacctggaga caaacctcgg acagccaaca     600

attcagagtt ttgaacaggt gggaacaaaa gtgaatgtga ccgtagaaga tgaacggact     660

ttagtcagaa ggaacaacac tttcctaagc ctccgggatg tttttggcaa ggacttaatt     720

tatacacttt attattggaa atcttcaagt tcaggaaaga aaacagccaa aacaaacact     780

aatgagtttt tgattgatgt ggataaagga gaaaactact gtttcagtgt tcaagcagtg     840

attccctccc gaacagttaa ccggaagagt acagacagcc cggtagagtg tatgggccag     900

gagaaagggg aattcagaga aatattctac atcattggag ctgtggtatt tgtggtcatc     960

atccttgtca tcatcctggc tatatctcta cacaagtgta gaaaggcagg agtggggcag    1020

agctggaagg agaactcccc actgaatgtt tcataaagga agcactgttg gagctactgc    1080

aaatgctata ttgcactgtg accgagaact tttaagagga tagaatacat ggaaacgcaa    1140

atgagtattt cggagcatga agaccctgga gttcaaaaaa ctcttgatat gacctgttat    1200

taccattagc attctggttt tgacatcagc attagtcact ttgaaatgta acaaatggta    1260

ctacaaccaa ttccaagttt taatttttaa caccatggca ccttttgcac ataacatgct    1320

ttagattata tattccgccg tcccaaaaaa aaaaaaaaaa aaaaaaaaaa aaaa          1374


