                         SEQUENCE LISTING

<110>  Max-Planck-Gesellschaft zur Frderung der Wissenschaften 
       e.V.
       University of Tampere
 
<120>  Alternative oxidase (AOX) prevents smoking-associated lung damage

<130>  W1009 PCT

<150>  EP 13 15 4287.0
<151>  2013-02-06

<150>  EP 12 19 9306.7
<151>  2012-12-21

<160>  15    

<170>  PatentIn version 3.5

<210>  1
<211>  369
<212>  PRT
<213>  Ciona intestinalis

<400>  1

Met Leu Ser Thr Gly Ser Lys Thr Phe Leu Phe Arg Pro Phe Leu Gly 
1               5                   10                  15      


Ser Cys His Ala Leu Gln Ser Gly Lys Leu Pro Cys Ser Asn Leu His 
            20                  25                  30          


Thr Thr Pro Thr Lys Ile Thr Val Lys Arg Tyr Leu Val Gly Tyr Ser 
        35                  40                  45              


Trp Ser Thr Gln Pro His Ser Arg Leu Leu His Ser Cys Gln Gln Leu 
    50                  55                  60                  


Lys Ile Asp Asp Lys Asn Lys Ser Glu His Phe Lys Ile Glu Thr Asn 
65                  70                  75                  80  


Asp Ser Thr Asp Glu Pro Asn Ile Glu Val Glu Asn Phe Pro His Phe 
                85                  90                  95      


Arg Glu Ala Lys Lys Ala Lys Glu Thr Gln Lys Gly Ser Ser Leu Ala 
            100                 105                 110         


Glu Ala Glu Glu His Pro Asp Val Glu Glu Gly Arg Ala Met Gln Asp 
        115                 120                 125             


Gly Gly Tyr Arg Leu Pro His Pro Ile Trp His Lys Gln Glu Leu Glu 
    130                 135                 140                 


Ser Val Arg Ile Ser His Arg Pro Pro Val Gly Lys Val Asp Lys Leu 
145                 150                 155                 160 


Ala Tyr Tyr Ser Val Gln Leu Leu Arg Thr Gly Phe Asp Val Phe Ser 
                165                 170                 175     


Gly Tyr Thr Leu Gly Thr Tyr Thr Gly Arg Leu Asp Glu Lys Gln Trp 
            180                 185                 190         


Val Lys Arg Ile Ile Phe Leu Glu Thr Ile Ala Gly Val Pro Gly Met 
        195                 200                 205             


Val Gly Ala Met Val Arg His Leu Val Ser Leu Arg Arg Leu Lys Arg 
    210                 215                 220                 


Asp His Gly Trp Ile His Thr Leu Leu Glu Glu Ala Glu Asn Glu Arg 
225                 230                 235                 240 


Met His Leu Met Thr Ala Met Arg Ile Ala Asn Pro Gly Ile Ile Met 
                245                 250                 255     


Arg Thr Ser Ile Val Val Ala Gln Gly Ile Phe Val Ser Gly Phe Ser 
            260                 265                 270         


Leu Ala Tyr Leu Ile Ser Pro Arg Phe Cys His Arg Phe Val Gly Tyr 
        275                 280                 285             


Leu Glu Glu Glu Ala Val Lys Thr Tyr Thr His Cys Leu Glu Glu Leu 
    290                 295                 300                 


Asp Ser Gly Asn Leu Lys Met Trp Cys Arg Met Lys Ala Pro Glu Ile 
305                 310                 315                 320 


Ala Val Glu Tyr Trp Lys Leu Pro Asp Asp Ala Met Met Arg Asp Val 
                325                 330                 335     


Ile Leu Ala Ile Arg Ala Asp Glu Ala His His Arg Ser Val Asn His 
            340                 345                 350         


Asp Leu Gly Ser Arg Lys Pro Asp Glu Gln Asn Pro Tyr Pro Pro Gly 
        355                 360                 365             


Gln 
    


<210>  2
<211>  1107
<212>  DNA
<213>  Ciona intestinalis

<400>  2
atgttgtcta ccggaagtaa aactttccta tttcgaccgt tcctgggctc atgccatgca       60

ctacaaagtg gaaaactacc atgttcaaat cttcatacaa ctcccacgaa aatcacagtg      120

aaaagatatt tggttggata tagttggtca actcagccac attccagatt acttcattca      180

tgtcaacaat taaagataga tgacaaaaat aaatccgagc attttaaaat tgaaacaaac      240

gattcaaccg atgaacccaa tatagaagtg gaaaacttcc ctcactttag agaagcaaaa      300

aaagcaaaag agacacaaaa aggaagctct cttgctgaag ctgaggagca tccggatgta      360

gaagaaggaa gagcgatgca agatggaggg tatagacttc ctcatcctat ctggcacaaa      420

caagaattag aatcagtgcg gatatcacat agacctcctg ttgggaaagt agacaaattg      480

gcttattaca gtgtacagtt acttcggact ggctttgatg ttttttctgg ttatactctc      540

ggtacctaca ctggacggct agatgagaaa cagtgggtca agagaattat atttttagaa      600

accattgctg gtgtaccagg aatggtcggt gccatggttc gtcacctggt ttccttacgt      660

agattaaagc gagaccacgg ttggattcac acattgcttg aggaagctga aaatgagaga      720

atgcacttaa tgactgcgat gagaattgct aaccctggta ttatcatgag gacgagtatt      780

gtggttgcac aaggaatctt tgtgtctgga ttttctttgg cttacttaat ctcaccacga      840

ttttgtcatc gttttgtcgg ctatctggaa gaggaagcag ttaagactta cacgcattgt      900

ttagaagaac ttgacagtgg aaacctaaaa atgtggtgcc gaatgaaagc gccagaaatt      960

gctgttgaat attggaaact ccctgacgat gcaatgatgc gagacgttat cctggcaatc     1020

cgagctgatg aagcacatca cagatcagtc aaccatgact tgggatcgag aaaaccagac     1080

gagcagaatc cttatccacc tggacaa                                         1107


<210>  3
<211>  318
<212>  PRT
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: AOX mature protein"

<400>  3

Gln Pro His Ser Arg Leu Leu His Ser Cys Gln Gln Leu Lys Ile Asp 
1               5                   10                  15      


Asp Lys Asn Lys Ser Glu His Phe Lys Ile Glu Thr Asn Asp Ser Thr 
            20                  25                  30          


Asp Glu Pro Asn Ile Glu Val Glu Asn Phe Pro His Phe Arg Glu Ala 
        35                  40                  45              


Lys Lys Ala Lys Glu Thr Gln Lys Gly Ser Ser Leu Ala Glu Ala Glu 
    50                  55                  60                  


Glu His Pro Asp Val Glu Glu Gly Arg Ala Met Gln Asp Gly Gly Tyr 
65                  70                  75                  80  


Arg Leu Pro His Pro Ile Trp His Lys Gln Glu Leu Glu Ser Val Arg 
                85                  90                  95      


Ile Ser His Arg Pro Pro Val Gly Lys Val Asp Lys Leu Ala Tyr Tyr 
            100                 105                 110         


Ser Val Gln Leu Leu Arg Thr Gly Phe Asp Val Phe Ser Gly Tyr Thr 
        115                 120                 125             


Leu Gly Thr Tyr Thr Gly Arg Leu Asp Glu Lys Gln Trp Val Lys Arg 
    130                 135                 140                 


Ile Ile Phe Leu Glu Thr Ile Ala Gly Val Pro Gly Met Val Gly Ala 
145                 150                 155                 160 


Met Val Arg His Leu Val Ser Leu Arg Arg Leu Lys Arg Asp His Gly 
                165                 170                 175     


Trp Ile His Thr Leu Leu Glu Glu Ala Glu Asn Glu Arg Met His Leu 
            180                 185                 190         


Met Thr Ala Met Arg Ile Ala Asn Pro Gly Ile Ile Met Arg Thr Ser 
        195                 200                 205             


Ile Val Val Ala Gln Gly Ile Phe Val Ser Gly Phe Ser Leu Ala Tyr 
    210                 215                 220                 


Leu Ile Ser Pro Arg Phe Cys His Arg Phe Val Gly Tyr Leu Glu Glu 
225                 230                 235                 240 


Glu Ala Val Lys Thr Tyr Thr His Cys Leu Glu Glu Leu Asp Ser Gly 
                245                 250                 255     


Asn Leu Lys Met Trp Cys Arg Met Lys Ala Pro Glu Ile Ala Val Glu 
            260                 265                 270         


Tyr Trp Lys Leu Pro Asp Asp Ala Met Met Arg Asp Val Ile Leu Ala 
        275                 280                 285             


Ile Arg Ala Asp Glu Ala His His Arg Ser Val Asn His Asp Leu Gly 
    290                 295                 300                 


Ser Arg Lys Pro Asp Glu Gln Asn Pro Tyr Pro Pro Gly Gln 
305                 310                 315             


<210>  4
<211>  954
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: AOX mature protein"

<400>  4
cagccacatt ccagattact tcattcatgt caacaattaa agatagatga caaaaataaa       60

tccgagcatt ttaaaattga aacaaacgat tcaaccgatg aacccaatat agaagtggaa      120

aacttccctc actttagaga agcaaaaaaa gcaaaagaga cacaaaaagg aagctctctt      180

gctgaagctg aggagcatcc ggatgtagaa gaaggaagag cgatgcaaga tggagggtat      240

agacttcctc atcctatctg gcacaaacaa gaattagaat cagtgcggat atcacataga      300

cctcctgttg ggaaagtaga caaattggct tattacagtg tacagttact tcggactggc      360

tttgatgttt tttctggtta tactctcggt acctacactg gacggctaga tgagaaacag      420

tgggtcaaga gaattatatt tttagaaacc attgctggtg taccaggaat ggtcggtgcc      480

atggttcgtc acctggtttc cttacgtaga ttaaagcgag accacggttg gattcacaca      540

ttgcttgagg aagctgaaaa tgagagaatg cacttaatga ctgcgatgag aattgctaac      600

cctggtatta tcatgaggac gagtattgtg gttgcacaag gaatctttgt gtctggattt      660

tctttggctt acttaatctc accacgattt tgtcatcgtt ttgtcggcta tctggaagag      720

gaagcagtta agacttacac gcattgttta gaagaacttg acagtggaaa cctaaaaatg      780

tggtgccgaa tgaaagcgcc agaaattgct gttgaatatt ggaaactccc tgacgatgca      840

atgatgcgag acgttatcct ggcaatccga gctgatgaag cacatcacag atcagtcaac      900

catgacttgg gatcgagaaa accagacgag cagaatcctt atccacctgg acaa            954


<210>  5
<211>  51
<212>  PRT
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: putative mitochondrial
       targeting sequence of AOX"

<400>  5

Met Leu Ser Thr Gly Ser Lys Thr Phe Leu Phe Arg Pro Phe Leu Gly 
1               5                   10                  15      


Ser Cys His Ala Leu Gln Ser Gly Lys Leu Pro Cys Ser Asn Leu His 
            20                  25                  30          


Thr Thr Pro Thr Lys Ile Thr Val Lys Arg Tyr Leu Val Gly Tyr Ser 
        35                  40                  45              


Trp Ser Thr 
    50      


<210>  6
<211>  63
<212>  PRT
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: mitochondrial 
       targeting peptide of ATP5B"

<400>  6

Met Leu Gly Phe Val Gly Arg Val Ala Ala Ala Pro Ala Ser Gly Ala 
1               5                   10                  15      


Leu Arg Arg Leu Thr Pro Ser Ala Ser Leu Pro Pro Ala Gln Leu Leu 
            20                  25                  30          


Leu Arg Ala Ala Pro Thr Ala Val His Pro Val Arg Asp Tyr Ala Ala 
        35                  40                  45              


Gln Thr Ser Pro Ser Pro Lys Ala Gly Ala Ala Thr Gly Arg Ile
    50                  55                  60          


<210>  7
<211>  30
<212>  PRT
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: mitochondrial 
       targeting peptide of ACO2"

<400>  7

Met Ala Pro Tyr Ser Leu Leu Val Thr Arg Leu Gln Lys Ala Leu Gly 
1               5                   10                  15      


Val Arg Gln Tyr His Val Ala Ser Val Leu Cys Gln Arg Ala 
            20                  25                  30  


<210>  8
<211>  189
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: mitochondrial 
       targeting sequence of ATP5B"

<400>  8
atgttggggt ttgtgggtcg ggtggccgct gctccggcct ccggggcctt gcggagactc       60

accccttcag cgtcgctgcc cccagctcag ctcttactgc gggccgctcc gacggcggtc      120

catcctgtca gggactatgc ggcgcaaaca tctccttcgc caaaagcagg cgccgccacc      180

gggcgcatc                                                              189


<210>  9
<211>  90
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: mitochondrial 
       targeting sequence of ACO2"

<400>  9
atggcgccct acagcctact ggtgactcgg ctgcagaaag ctctgggtgt gcggcagtac       60

catgtggcct cagtcctgtg ccaacgggcc                                        90


<210>  10
<211>  155
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: 3'UTR of ATP5B"

<400>  10
ggggtctttg tcctctgtac tgtctctctc cttgccccta acccaaaaag cttcattttt       60

ctgtgtaggc tgcacaagag ccttgattga agatatattc tttctgaaca gtatttaagg      120

tttccaataa aatgtacacc cctcagaaaa aaaaa                                 155


<210>  11
<211>  379
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: 3'UTR of ACO2"

<400>  11
gggcagtgcc tccccgcccc gccgctggcg tcaagttcag ctccacgtgt gccatcagtg       60

gatccgatcc gtccagccat ggcttcctat tccaagatgg tgtgaccaga catgcttcct      120

gctccccgct tagcccacgg agtgactgtg gttgtggtgg gggggttctt aaaataactt      180

tttagccccc gtcttcctat tttgagtttg gttcagatct taagcagctc catgcaactg      240

tatttatttt tgatgacaag actcccatct aaagtttttc tcctgcctga tcatttcatt      300

ggtggctgaa ggattctaga gaaccttttg ttcttgcaag gaaaacaaga atccaaaacc      360

agtgactgtt ctgtgaaaa                                                   379


<210>  12
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: probe for AOX"

<400>  12
cttgacccac tgtttctcat ctagccg                                           27


<210>  13
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: probe for Tfam"

<400>  13
cccaatgaca actccgtctt                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: probe for 12S rRNA"

<400>  14
catgggctac accttgacct                                                   20


<210>  15
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  /note="Description of artificial sequence: probe for 18S rRNA"

<400>  15
tcgaaccctg attccccgtc accc                                              24


