                         SEQUENCE LISTING

<110>  Roche Diagnostics GmbH
       F. Hoffmann-La Roche AG
 
<120>  Improved Calibration of High Resolution Melting

<130>  31410 WO

<150>  EP 13150790.7
<151>  2013-01-10

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence designed experiment dependent.


<220>
<221>  Cy5
<222>  (1)..(1)
<223>  Fluorescent Dye Cy5 is covalently bound on 5' end of 
       oligonucleotide.

<220>
<221>  Phosphate
<222>  (25)..(25)
<223>  Oligonucleotide is phosphorylated on 3' end.

<400>  1
tgggggtggg ggtgggggtg ggggt                                             25


<210>  2
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence designed experiment dependent.


<220>
<221>  BHQ-3
<222>  (25)..(25)
<223>  Quencher BHQ-3 covalently bound to 3'-end of the oligonucleotide.

<400>  2
acccccaccc ccacccccac cccca                                             25


<210>  3
<211>  17
<212>  DNA
<213>  Homo sapiens

<400>  3
gatggctgaa ctctggc                                                      17


<210>  4
<211>  17
<212>  DNA
<213>  Homo sapiens

<400>  4
cgacttggga ctgcttc                                                      17


<210>  5
<211>  18
<212>  DNA
<213>  Homo sapiens

<400>  5
cgtttctccc tcatgacg                                                     18


<210>  6
<211>  21
<212>  DNA
<213>  Homo sapiens

<400>  6
tcagtgatat ggagtagggt c                                                 21


<210>  7
<211>  19
<212>  DNA
<213>  Homo sapiens

<400>  7
gggctatgga agtcgagta                                                    19


<210>  8
<211>  19
<212>  DNA
<213>  Homo sapiens

<400>  8
cgtcccctgt atccatacc                                                    19


