                               SEQUENCE LISTING

<110> THE BRIGHAM AND WOMEN'S HOSPITAL, INC.
 
<120> USING GALECTIN-BINDING CARBOHYDRATES AS PREDICTORS OF MELANOMA 
      PROGRESSION AND METASTASIS

<130> 043214-076642-PCT

<140>
<141>

<150> 61/753,242
<151> 2013-01-16

<150> 61/753,228
<151> 2013-01-16

<160> 9     

<170> PatentIn version 3.5

<210> 1
<211> 364
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 1
Met Ala Cys Gly Leu Val Ala Ser Asn Leu Asn Leu Lys Pro Gly Glu 
1               5                   10                  15      


Cys Leu Lys Val Arg Gly Glu Val Ala Ser Asp Ala Lys Ser Phe Val 
            20                  25                  30          


Leu Asn Leu Gly Lys Asp Ser Asn Asn Leu Cys Leu His Phe Asn Pro 
        35                  40                  45              


Arg Phe Asn Ala His Gly Asp Ala Asn Thr Ile Val Cys Asn Thr Lys 
    50                  55                  60                  


Glu Asp Gly Thr Trp Gly Thr Glu His Arg Glu Pro Ala Phe Pro Phe 
65                  70                  75                  80  


Gln Pro Gly Ser Ile Thr Glu Val Cys Ile Thr Phe Asp Gln Ala Asp 
                85                  90                  95      


Leu Thr Ile Lys Leu Pro Asp Gly His Glu Phe Lys Phe Pro Asn Arg 
            100                 105                 110         


Leu Asn Met Glu Ala Ile Asn Tyr Met Ala Ala Asp Gly Asp Phe Lys 
        115                 120                 125             


Ile Lys Cys Val Ala Phe Glu Arg Ser Asp Lys Thr His Thr Cys Pro 
    130                 135                 140                 


Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe 
145                 150                 155                 160 


Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val 
                165                 170                 175     


Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe 
            180                 185                 190         


Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro 
        195                 200                 205             


Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr 
    210                 215                 220                 


Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val 
225                 230                 235                 240 


Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala 
                245                 250                 255     


Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg 
            260                 265                 270         


Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly 
        275                 280                 285             


Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro 
    290                 295                 300                 


Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser 
305                 310                 315                 320 


Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln 
                325                 330                 335     


Gly Asn Val Phe Ser Cys Ser Val Met His Glu Gly Leu His Asn His 
            340                 345                 350         


Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
        355                 360                 


<210> 2
<211> 4132
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 2
ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg       60

agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa      120

actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt      180

atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac      240

agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct acctgaggcc      300

gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg      360

cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc      420

cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg cttgctcaac      480

tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg tgaccggcgc      540

ctacctgaga tcaccggtga attcgatatc tcgagcacca tggttagatc tgacaaaact      600

cacacatgcc caccgtgccc agcacctgaa ctcctggggg gaccgtcagt cttcctcttc      660

cccccaaaac ccaaggacac cctcatgatc tcccggaccc ctgaggtcac atgcgtggtg      720

gtggacgtga gccacgaaga ccctgaggtc aagttcaact ggtacgtgga cggcgtggag      780

gtgcataatg ccaagacaaa gccgcgggag gagcagtaca acagcacgta ccgtgtggtc      840

agcgtcctca ccgtcctgca ccaggactgg ctgaatggca aggagtacaa gtgcaaggtc      900

tccaacaaag ccctcccagc ccccatcgag aaaaccatct ccaaagccaa agggcagccc      960

cgagaaccac aggtgtacac cctgccccca tcccgggagg agatgaccaa gaaccaggtc     1020

agcctgacct gcctggtcaa aggcttctat cccagcgaca tcgccgtgga gtgggagagc     1080

aatgggcagc cggagaacaa ctacaagacc acgcctcccg tgctggactc cgacggctcc     1140

ttcttcctct acagcaagct caccgtggac aagagcaggt ggcagcaggg gaacgtcttc     1200

tcatgctccg tgatgcacga ggctctgcac aaccactaca cgcagaagag cctctccctg     1260

tctccgggta aatgagtgct agctggccag acatgataag atacattgat gagtttggac     1320

aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg     1380

ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt     1440

ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca     1500

aatgtggtat ggaattaatt ctaaaataca gcatagcaaa actttaacct ccaaatcaag     1560

cctctacttg aatccttttc tgagggatga ataaggcata ggcatcaggg gctgttgcca     1620

atgtgcatta gctgtttgca gcctcacctt ctttcatgga gtttaagata tagtgtattt     1680

tcccaaggtt tgaactagct cttcatttct ttatgtttta aatgcactga cctcccacat     1740

tcccttttta gtaaaatatt cagaaataat ttaaatacat cattgcaatg aaaataaatg     1800

ttttttatta ggcagaatcc agatgctcaa ggcccttcat aatatccccc agtttagtag     1860

ttggacttag ggaacaaagg aacctttaat agaaattgga cagcaagaaa gcgagcttct     1920

agcttatcct cagtcctgct cctctgccac aaagtgcacg cagttgccgg ccgggtcgcg     1980

cagggcgaac tcccgccccc acggctgctc gccgatctcg gtcatggccg gcccggaggc     2040

gtcccggaag ttcgtggaca cgacctccga ccactcggcg tacagctcgt ccaggccgcg     2100

cacccacacc caggccaggg tgttgtccgg caccacctgg tcctggaccg cgctgatgaa     2160

cagggtcacg tcgtcccgga ccacaccggc gaagtcgtcc tccacgaagt cccgggagaa     2220

cccgagccgg tcggtccaga actcgaccgc tccggcgacg tcgcgcgcgg tgagcaccgg     2280

aacggcactg gtcaacttgg ccatgatggc tcctcctgtc aggagaggaa agagaagaag     2340

gttagtacaa ttgctatagt gagttgtatt atactatgca gatatactat gccaatgatt     2400

aattgtcaaa ctagggctgc agggttcata gtgccacttt tcctgcactg ccccatctcc     2460

tgcccaccct ttcccaggca tagacagtca gtgacttacc aaactcacag gagggagaag     2520

gcagaagctt gagacagacc cgcgggaccg ccgaactgcg aggggacgtg gctagggcgg     2580

cttcttttat ggtgcgccgg ccctcggagg cagggcgctc ggggaggcct agcggccaat     2640

ctgcggtggc aggaggcggg gccgaaggcc gtgcctgacc aatccggagc acataggagt     2700

ctcagccccc cgccccaaag caaggggaag tcacgcgcct gtagcgccag cgtgttgtga     2760

aatgggggct tgggggggtt ggggccctga ctagtcaaaa caaactccca ttgacgtcaa     2820

tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact     2880

gccaaaaccg catcatcatg gtaatagcga tgactaatac gtagatgtac tgccaagtag     2940

gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga     3000

cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt     3060

accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa     3120

catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat     3180

ttaccgtaag ttatgtaacg cctgcaggtt aattaagaac atgtgagcaa aaggccagca     3240

aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc     3300

tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata     3360

aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc     3420

gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc     3480

acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga     3540

accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc     3600

ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag     3660

gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag     3720

aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag     3780

ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca     3840

gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga     3900

cgctcagtgg aacgaaaact cacgttaagg gattttggtc atggctagtt aattaacatt     3960

taaatcagcg gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg     4020

tgtgaatcgt aactaacata cgctctccat caaaacaaaa cgaaacaaaa caaactagca     4080

aaataggctg tccccagtgc aagtgcaggt gccagaacat ttctctatcg aa             4132


<210> 3
<211> 227
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 3
Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
1               5                   10                  15      


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
            20                  25                  30          


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
        35                  40                  45              


Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
    50                  55                  60                  


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 
65                  70                  75                  80  


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
                85                  90                  95      


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
            100                 105                 110         


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
        115                 120                 125             


Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser 
    130                 135                 140                 


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
145                 150                 155                 160 


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
                165                 170                 175     


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
            180                 185                 190         


Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
        195                 200                 205             


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
    210                 215                 220                 


Pro Gly Lys 
225         


<210> 4
<211> 124
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 4
Met Ala Lys Leu Thr Ser Ala Val Pro Val Leu Thr Ala Arg Asp Val 
1               5                   10                  15      


Ala Gly Ala Val Glu Phe Trp Thr Asp Arg Leu Gly Phe Ser Arg Asp 
            20                  25                  30          


Phe Val Glu Asp Asp Phe Ala Gly Val Val Arg Asp Asp Val Thr Leu 
        35                  40                  45              


Phe Ile Ser Ala Val Gln Asp Gln Val Val Pro Asp Asn Thr Leu Ala 
    50                  55                  60                  


Trp Val Trp Val Arg Gly Leu Asp Glu Leu Tyr Ala Glu Trp Ser Glu 
65                  70                  75                  80  


Val Val Ser Thr Asn Phe Arg Asp Ala Ser Gly Pro Ala Met Thr Glu 
                85                  90                  95      


Ile Gly Glu Gln Pro Trp Gly Arg Glu Phe Ala Leu Arg Asp Pro Ala 
            100                 105                 110         


Gly Asn Cys Val His Phe Val Ala Glu Glu Gln Asp 
        115                 120                 


<210> 5
<211> 408
<212> DNA
<213> Mus sp.

<400> 5
atggcctgtg gtctggtcgc cagcaacctg aatctcaaac ctggggaatg tctcaaagtt       60

cggggagagg tggcctcgga cgccaagagc tttgtgctga acctgggaaa agacagcaac      120

aacctgtgcc tacacttcaa tcctcgcttc aatgcccatg gagacgccaa caccattgtg      180

tgtaacacca aggaagatgg gacctgggga accgaacacc gggaacctgc cttccccttc      240

cagcccggga gcatcacaga ggtgtgcatc acctttgacc aggctgacct gaccatcaag      300

ctgccagacg gacatgaatt caagttcccc aaccgcctca acatggaggc catcaactac      360

atggcggcgg atggagactt caagattaag tgcgtggcct ttgagaga                   408


<210> 6
<211> 1035
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide


<220>
<221> modified_base
<222> (1)..(11)
<223> a, c, t, g, unknown or other

<220>
<221> modified_base
<222> (15)..(22)
<223> a, c, t, g, unknown or other

<220>
<221> modified_base
<222> (783)..(783)
<223> a, c, t, g, unknown or other

<220>
<221> modified_base
<222> (858)..(858)
<223> a, c, t, g, unknown or other

<220>
<221> modified_base
<222> (937)..(937)
<223> a, c, t, g, unknown or other

<220>
<221> modified_base
<222> (940)..(940)
<223> a, c, t, g, unknown or other

<220>
<221> modified_base
<222> (971)..(972)
<223> a, c, t, g, unknown or other

<220>
<221> modified_base
<222> (977)..(977)
<223> a, c, t, g, unknown or other

<220>
<221> modified_base
<222> (980)..(980)
<223> a, c, t, g, unknown or other

<220>
<221> modified_base
<222> (982)..(982)
<223> a, c, t, g, unknown or other

<220>
<221> modified_base
<222> (990)..(990)
<223> a, c, t, g, unknown or other

<220>
<221> modified_base
<222> (998)..(998)
<223> a, c, t, g, unknown or other

<220>
<221> modified_base
<222> (1001)..(1002)
<223> a, c, t, g, unknown or other

<220>
<221> modified_base
<222> (1005)..(1006)
<223> a, c, t, g, unknown or other

<220>
<221> modified_base
<222> (1008)..(1008)
<223> a, c, t, g, unknown or other

<220>
<221> modified_base
<222> (1010)..(1010)
<223> a, c, t, g, unknown or other

<220>
<221> modified_base
<222> (1015)..(1016)
<223> a, c, t, g, unknown or other

<220>
<221> modified_base
<222> (1019)..(1019)
<223> a, c, t, g, unknown or other

<220>
<221> modified_base
<222> (1024)..(1024)
<223> a, c, t, g, unknown or other

<220>
<221> modified_base
<222> (1027)..(1028)
<223> a, c, t, g, unknown or other

<220>
<221> modified_base
<222> (1030)..(1033)
<223> a, c, t, g, unknown or other

<400> 6
nnnnnnnnnn ntgcnnnnnn nnatccagct gtgaccggcg cctacctgag atcaccggtg       60

aattcgatat ctcgaggcca cccgtctctc gggtggagtc ttctgactgc tggtggagca      120

ggtctcagga atctcttcgc ttcagcttca atcatggcct gtggtctggt cgccagcaac      180

ctgaatctca aacctgggga atgtctcaaa gttcggggag aggtggcctc ggacgccaag      240

agctttgtgc tgaacctggg aaaagacagc aacaacctgt gcctacactt caatcctcgc      300

ttcaatgccc atggagacgc caacaccatt gtgtgtaaca ccaaggaaga tgggacctgg      360

ggaaccgaac accgggaacc tgccttcccc ttccagcccg ggagcatcac agaggtgtgc      420

atcacctttg accaggctga cctgaccatc aagctgccag acggacatga attcaagttc      480

cccaaccgcc tcaacatgga ggccatcaac tacatggcgg cggatggaga cttcaagatt      540

aagtgcgtgg cctttgagag atctgacaaa actcacacat gcccaccgtg cccagcacct      600

gaactcctgg ggggaccgtc agtcttcctc ttccccccaa aacccaagga caccctcatg      660

atctcccgga cccctgaggt cacatgcgtg gtggtggacg tgagccacga agaccctgag      720

gtcaagttca actggtacgt ggacggcgtg gaggtgcata atgccaagac aaagccgcgg      780

gangagcagt acaacagcac gtaccgtgtg gtcagcgtcc tcaccgtcct gcaccaggac      840

tggctgaatg gcaagganta caagtgcaag gtctccaaca aagccctccc agcccccatc      900

gagaaaacca tctccaaagc caaagggcag ccccganaan cacaggtgta caccctgccc      960

ccatcccggg nngagangan cnagaaccan gtcagccnga nntgnntngn caaannctnc     1020

tatnccnncn nnntc                                                      1035


<210> 7
<211> 135
<212> PRT
<213> Mus sp.

<400> 7
Met Ala Cys Gly Leu Val Ala Ser Asn Leu Asn Leu Lys Pro Gly Glu 
1               5                   10                  15      


Cys Leu Lys Val Arg Gly Glu Val Ala Ser Asp Ala Lys Ser Phe Val 
            20                  25                  30          


Leu Asn Leu Gly Lys Asp Ser Asn Asn Leu Cys Leu His Phe Asn Pro 
        35                  40                  45              


Arg Phe Asn Ala His Gly Asp Ala Asn Thr Ile Val Cys Asn Thr Lys 
    50                  55                  60                  


Glu Asp Gly Thr Trp Gly Thr Glu His Arg Glu Pro Ala Phe Pro Phe 
65                  70                  75                  80  


Gln Pro Gly Ser Ile Thr Glu Val Cys Ile Thr Phe Asp Gln Ala Asp 
                85                  90                  95      


Leu Thr Ile Lys Leu Pro Asp Gly His Glu Phe Lys Phe Pro Asn Arg 
            100                 105                 110         


Leu Asn Met Glu Ala Ile Asn Tyr Met Ala Ala Asp Gly Asp Phe Lys 
        115                 120                 125             


Ile Lys Cys Val Ala Phe Glu 
    130                 135 


<210> 8
<211> 135
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 8
Met Ala Cys Gly Leu Val Ala Ser Asn Leu Asn Leu Lys Pro Gly Glu 
1               5                   10                  15      


Cys Leu Lys Val Arg Gly Glu Val Ala Ser Asp Ala Lys Ser Phe Val 
            20                  25                  30          


Leu Asn Leu Gly Lys Asp Ser Asn Asn Leu Cys Leu His Phe Asn Pro 
        35                  40                  45              


Arg Phe Asn Ala His Gly Asp Ala Asn Thr Ile Val Cys Asn Thr Lys 
    50                  55                  60                  


Glu Asp Gly Thr Gly Gly Thr Glu His Arg Glu Pro Ala Phe Pro Phe 
65                  70                  75                  80  


Gln Pro Gly Ser Ile Thr Glu Val Cys Ile Thr Phe Asp Gln Ala Asp 
                85                  90                  95      


Leu Thr Ile Lys Leu Pro Asp Gly His Glu Phe Lys Phe Pro Asn Arg 
            100                 105                 110         


Leu Asn Met Glu Ala Ile Asn Tyr Met Ala Ala Asp Gly Asp Phe Lys 
        115                 120                 125             


Ile Lys Cys Val Ala Phe Glu 
    130                 135 


<210> 9
<211> 135
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 9
Met Ala Cys Gly Leu Val Ala Ser Asn Leu Asn Leu Lys Pro Gly Glu 
1               5                   10                  15      


Cys Leu Lys Val Arg Gly Glu Val Ala Ser Asp Ala Lys Ser Phe Val 
            20                  25                  30          


Leu Asn Leu Gly Lys Asp Ser Asn Asn Leu Cys Leu Leu Phe Asn Pro 
        35                  40                  45              


Arg Phe Asn Ala His Gly Asp Ala Asn Thr Ile Val Cys Asn Thr Lys 
    50                  55                  60                  


Glu Asp Gly Thr Gly Gly Thr Glu His Arg Glu Pro Ala Phe Pro Phe 
65                  70                  75                  80  


Gln Pro Gly Ser Ile Thr Glu Val Cys Ile Thr Phe Asp Gln Ala Asp 
                85                  90                  95      


Leu Thr Ile Lys Leu Pro Asp Gly His Glu Phe Lys Phe Pro Asn Arg 
            100                 105                 110         


Leu Asn Met Glu Ala Ile Asn Tyr Met Ala Ala Asp Gly Asp Phe Lys 
        115                 120                 125             


Ile Lys Cys Val Ala Phe Glu 
    130                 135 


