                         SEQUENCE LISTING

<110>  Danmarks Tekniske Universitet
       Statens Serum Institut
       Jungersen, Gregers
       Thakur, Aneesh
       Mikkelsen, Heidi
       Aagaard, Claus
       Andersen, Peter Lawtz
 
<120>  A single or multistage Mycobacterium avium subsp. 
       paratuberculosis subunit vaccine

<130>  P1406EP00

<150>  EP13152908.3
<151>  2013-01-28

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  1836
<212>  DNA
<213>  Mycobacterium avium


<220>
<221>  CDS
<222>  (1)..(1836)
<223>  MAP3694 gene encoding MAP3694 polypeptide

<400>  1
atg agc cac tac aag agc aac gtc cgc gac cag gaa ttc aac ctg ttc         48
Met Ser His Tyr Lys Ser Asn Val Arg Asp Gln Glu Phe Asn Leu Phe           
1               5                   10                  15                

gaa gtg ctg ggc gtt gac aag gct tta ggg caa ggc gag ttc agc gat         96
Glu Val Leu Gly Val Asp Lys Ala Leu Gly Gln Gly Glu Phe Ser Asp           
            20                  25                  30                    

ctg gac gcc gac acg gcc cgc gag atg ttg aac gaa atc agc cgg ctg        144
Leu Asp Ala Asp Thr Ala Arg Glu Met Leu Asn Glu Ile Ser Arg Leu           
        35                  40                  45                        

gcc gag ggg ccc atc gcc gac tcc ttc gtc gag ggc gac cgc aac ccg        192
Ala Glu Gly Pro Ile Ala Asp Ser Phe Val Glu Gly Asp Arg Asn Pro           
    50                  55                  60                            

ccg gtg ttc gac ccg aag acg cac tcc gtg acg ctg ccg gaa tcg ttc        240
Pro Val Phe Asp Pro Lys Thr His Ser Val Thr Leu Pro Glu Ser Phe           
65                  70                  75                  80            

aag aag tcc gtc cac gcc gtc atc gag gcc ggg tgg gac aag gta ggc        288
Lys Lys Ser Val His Ala Val Ile Glu Ala Gly Trp Asp Lys Val Gly           
                85                  90                  95                

atc gac gag gcc ctc ggc ggg gtc gcc atg ccc aag gcg ctg ctg tgg        336
Ile Asp Glu Ala Leu Gly Gly Val Ala Met Pro Lys Ala Leu Leu Trp           
            100                 105                 110                   

gcg ctg cac gag cac atc ctg ggc gcc aac ccg gcg gtc tgg atg tac        384
Ala Leu His Glu His Ile Leu Gly Ala Asn Pro Ala Val Trp Met Tyr           
        115                 120                 125                       

gcc ggc ggc gcg ggc ttc gcc aac atc ctc tac cac ctg ggc acc gac        432
Ala Gly Gly Ala Gly Phe Ala Asn Ile Leu Tyr His Leu Gly Thr Asp           
    130                 135                 140                           

gag cag aag aag tgg gcc gtg atg gcc gcc gag cgc ggc tgg ggt tcg        480
Glu Gln Lys Lys Trp Ala Val Met Ala Ala Glu Arg Gly Trp Gly Ser           
145                 150                 155                 160           

acc atg gtg ctc acc gag ccg gac gcg ggt tcc gac gtc ggc gcc ggg        528
Thr Met Val Leu Thr Glu Pro Asp Ala Gly Ser Asp Val Gly Ala Gly           
                165                 170                 175               

cgc acc aag gcg gtc aag cag gac gac ggc tcc tgg cac atc gac ggt        576
Arg Thr Lys Ala Val Lys Gln Asp Asp Gly Ser Trp His Ile Asp Gly           
            180                 185                 190                   

gtg aag cga ttc atc acc tcg gcc gac tcc gac gac ctg ttc gag aac        624
Val Lys Arg Phe Ile Thr Ser Ala Asp Ser Asp Asp Leu Phe Glu Asn           
        195                 200                 205                       

atc ttc cac ctg gtg ctg gcc cgc ccc gag ggc gcc ggc ccg ggc acc        672
Ile Phe His Leu Val Leu Ala Arg Pro Glu Gly Ala Gly Pro Gly Thr           
    210                 215                 220                           

aag ggc ctg tcg ctg ttc ttc gtg ccc aag ttc ctg ttc gac ttc gag        720
Lys Gly Leu Ser Leu Phe Phe Val Pro Lys Phe Leu Phe Asp Phe Glu           
225                 230                 235                 240           

acc ggt gag ctc ggc gag cgc aac ggc gtc ttc gtc acc aat gtc gag        768
Thr Gly Glu Leu Gly Glu Arg Asn Gly Val Phe Val Thr Asn Val Glu           
                245                 250                 255               

cac aag atg ggc ctg aag gtg tcg gcg acc tgc gag ctg agc ttc ggc        816
His Lys Met Gly Leu Lys Val Ser Ala Thr Cys Glu Leu Ser Phe Gly           
            260                 265                 270                   

cag cac gac gtc ccg gcc aag ggc tgg ctg gtc ggc gag gtg cac aac        864
Gln His Asp Val Pro Ala Lys Gly Trp Leu Val Gly Glu Val His Asn           
        275                 280                 285                       

ggg atc gcg cag atg ttc gag gtc atc gag cag gcc cgc atg atg gtg        912
Gly Ile Ala Gln Met Phe Glu Val Ile Glu Gln Ala Arg Met Met Val           
    290                 295                 300                           

ggc acc aag gcc atc gcg acc ctg tcg acc ggt tac ctg aac gcg ttg        960
Gly Thr Lys Ala Ile Ala Thr Leu Ser Thr Gly Tyr Leu Asn Ala Leu           
305                 310                 315                 320           

gag tac gcc aag tct cgc gtc cag ggc gcc gac atg acc cag atg acc       1008
Glu Tyr Ala Lys Ser Arg Val Gln Gly Ala Asp Met Thr Gln Met Thr           
                325                 330                 335               

gac aag acc gca ccg cgg gtg acc atc acg cac cac ccg gac gtg cgg       1056
Asp Lys Thr Ala Pro Arg Val Thr Ile Thr His His Pro Asp Val Arg           
            340                 345                 350                   

cgg tcg ctg atg acc cag aag gcc tac gcc gag ggt ctg cgc gcg ctg       1104
Arg Ser Leu Met Thr Gln Lys Ala Tyr Ala Glu Gly Leu Arg Ala Leu           
        355                 360                 365                       

tac ctg ttc acc tcg acc tac cag gac gcg gcg gtc gcc gag gcg ctc       1152
Tyr Leu Phe Thr Ser Thr Tyr Gln Asp Ala Ala Val Ala Glu Ala Leu           
    370                 375                 380                           

tac ggg gtg gac gcc gaa ctg gcg gtc aag gtc aac gac ctg atg ctg       1200
Tyr Gly Val Asp Ala Glu Leu Ala Val Lys Val Asn Asp Leu Met Leu           
385                 390                 395                 400           

ccg gtg gtc aag ggc gtc ggc tcc gag cag gcc tac gcc aag ctc acc       1248
Pro Val Val Lys Gly Val Gly Ser Glu Gln Ala Tyr Ala Lys Leu Thr           
                405                 410                 415               

gag agc ctg cag acc ttc ggt ggc tcc ggc ttc ctg cag gac tac ccg       1296
Glu Ser Leu Gln Thr Phe Gly Gly Ser Gly Phe Leu Gln Asp Tyr Pro           
            420                 425                 430                   

att gag cag tac atc cgc gac tcc aag atc gac tcg ctc tac gag ggc       1344
Ile Glu Gln Tyr Ile Arg Asp Ser Lys Ile Asp Ser Leu Tyr Glu Gly           
        435                 440                 445                       

acc acg gcc atc cag gcg cag gac ttc ttc ttc cgc aag atc gtc cgc       1392
Thr Thr Ala Ile Gln Ala Gln Asp Phe Phe Phe Arg Lys Ile Val Arg           
    450                 455                 460                           

gac aag ggt gtg gcg ctg gcc cac gtg tcc gag cag atc cag aag ttc       1440
Asp Lys Gly Val Ala Leu Ala His Val Ser Glu Gln Ile Gln Lys Phe           
465                 470                 475                 480           

gtc gac agc gag tcc ggc aac ggc cgg ctc aag tcc gag cgc gaa ctg       1488
Val Asp Ser Glu Ser Gly Asn Gly Arg Leu Lys Ser Glu Arg Glu Leu           
                485                 490                 495               

ctg gcc aag gcg ctg gcg gac gtg cag ggc atg gcg acc gcg ctg acc       1536
Leu Ala Lys Ala Leu Ala Asp Val Gln Gly Met Ala Thr Ala Leu Thr           
            500                 505                 510                   

ggc tac ctg atg gcc gcc cag gag gac gtg acc agc ctg tac aag gtg       1584
Gly Tyr Leu Met Ala Ala Gln Glu Asp Val Thr Ser Leu Tyr Lys Val           
        515                 520                 525                       

ggc ctg ggt tcg gtg cgg ttc ctg atg agc gtc ggt gac ctg atc atc       1632
Gly Leu Gly Ser Val Arg Phe Leu Met Ser Val Gly Asp Leu Ile Ile           
    530                 535                 540                           

ggc tgg ttg ctg cag cgt cag gcg gcg gtg gcc gtg gcg gcg ctg gac       1680
Gly Trp Leu Leu Gln Arg Gln Ala Ala Val Ala Val Ala Ala Leu Asp           
545                 550                 555                 560           

gcc ggc gcc agc ggg gag gac cgg tcc ttc tac gag ggc aag atc gcg       1728
Ala Gly Ala Ser Gly Glu Asp Arg Ser Phe Tyr Glu Gly Lys Ile Ala           
                565                 570                 575               

gtg gcg tcg ttc ttc gcg aag aac ttc ctg ccg atg ctg acc agc acc       1776
Val Ala Ser Phe Phe Ala Lys Asn Phe Leu Pro Met Leu Thr Ser Thr           
            580                 585                 590                   

cgc gag gtc atc gag acg ctg gac aac gac atc atg gag ctc gac gag       1824
Arg Glu Val Ile Glu Thr Leu Asp Asn Asp Ile Met Glu Leu Asp Glu           
        595                 600                 605                       

gcg gcc ttc taa                                                       1836
Ala Ala Phe                                                               
    610                                                                   


<210>  2
<211>  611
<212>  PRT
<213>  Mycobacterium avium

<400>  2

Met Ser His Tyr Lys Ser Asn Val Arg Asp Gln Glu Phe Asn Leu Phe 
1               5                   10                  15      


Glu Val Leu Gly Val Asp Lys Ala Leu Gly Gln Gly Glu Phe Ser Asp 
            20                  25                  30          


Leu Asp Ala Asp Thr Ala Arg Glu Met Leu Asn Glu Ile Ser Arg Leu 
        35                  40                  45              


Ala Glu Gly Pro Ile Ala Asp Ser Phe Val Glu Gly Asp Arg Asn Pro 
    50                  55                  60                  


Pro Val Phe Asp Pro Lys Thr His Ser Val Thr Leu Pro Glu Ser Phe 
65                  70                  75                  80  


Lys Lys Ser Val His Ala Val Ile Glu Ala Gly Trp Asp Lys Val Gly 
                85                  90                  95      


Ile Asp Glu Ala Leu Gly Gly Val Ala Met Pro Lys Ala Leu Leu Trp 
            100                 105                 110         


Ala Leu His Glu His Ile Leu Gly Ala Asn Pro Ala Val Trp Met Tyr 
        115                 120                 125             


Ala Gly Gly Ala Gly Phe Ala Asn Ile Leu Tyr His Leu Gly Thr Asp 
    130                 135                 140                 


Glu Gln Lys Lys Trp Ala Val Met Ala Ala Glu Arg Gly Trp Gly Ser 
145                 150                 155                 160 


Thr Met Val Leu Thr Glu Pro Asp Ala Gly Ser Asp Val Gly Ala Gly 
                165                 170                 175     


Arg Thr Lys Ala Val Lys Gln Asp Asp Gly Ser Trp His Ile Asp Gly 
            180                 185                 190         


Val Lys Arg Phe Ile Thr Ser Ala Asp Ser Asp Asp Leu Phe Glu Asn 
        195                 200                 205             


Ile Phe His Leu Val Leu Ala Arg Pro Glu Gly Ala Gly Pro Gly Thr 
    210                 215                 220                 


Lys Gly Leu Ser Leu Phe Phe Val Pro Lys Phe Leu Phe Asp Phe Glu 
225                 230                 235                 240 


Thr Gly Glu Leu Gly Glu Arg Asn Gly Val Phe Val Thr Asn Val Glu 
                245                 250                 255     


His Lys Met Gly Leu Lys Val Ser Ala Thr Cys Glu Leu Ser Phe Gly 
            260                 265                 270         


Gln His Asp Val Pro Ala Lys Gly Trp Leu Val Gly Glu Val His Asn 
        275                 280                 285             


Gly Ile Ala Gln Met Phe Glu Val Ile Glu Gln Ala Arg Met Met Val 
    290                 295                 300                 


Gly Thr Lys Ala Ile Ala Thr Leu Ser Thr Gly Tyr Leu Asn Ala Leu 
305                 310                 315                 320 


Glu Tyr Ala Lys Ser Arg Val Gln Gly Ala Asp Met Thr Gln Met Thr 
                325                 330                 335     


Asp Lys Thr Ala Pro Arg Val Thr Ile Thr His His Pro Asp Val Arg 
            340                 345                 350         


Arg Ser Leu Met Thr Gln Lys Ala Tyr Ala Glu Gly Leu Arg Ala Leu 
        355                 360                 365             


Tyr Leu Phe Thr Ser Thr Tyr Gln Asp Ala Ala Val Ala Glu Ala Leu 
    370                 375                 380                 


Tyr Gly Val Asp Ala Glu Leu Ala Val Lys Val Asn Asp Leu Met Leu 
385                 390                 395                 400 


Pro Val Val Lys Gly Val Gly Ser Glu Gln Ala Tyr Ala Lys Leu Thr 
                405                 410                 415     


Glu Ser Leu Gln Thr Phe Gly Gly Ser Gly Phe Leu Gln Asp Tyr Pro 
            420                 425                 430         


Ile Glu Gln Tyr Ile Arg Asp Ser Lys Ile Asp Ser Leu Tyr Glu Gly 
        435                 440                 445             


Thr Thr Ala Ile Gln Ala Gln Asp Phe Phe Phe Arg Lys Ile Val Arg 
    450                 455                 460                 


Asp Lys Gly Val Ala Leu Ala His Val Ser Glu Gln Ile Gln Lys Phe 
465                 470                 475                 480 


Val Asp Ser Glu Ser Gly Asn Gly Arg Leu Lys Ser Glu Arg Glu Leu 
                485                 490                 495     


Leu Ala Lys Ala Leu Ala Asp Val Gln Gly Met Ala Thr Ala Leu Thr 
            500                 505                 510         


Gly Tyr Leu Met Ala Ala Gln Glu Asp Val Thr Ser Leu Tyr Lys Val 
        515                 520                 525             


Gly Leu Gly Ser Val Arg Phe Leu Met Ser Val Gly Asp Leu Ile Ile 
    530                 535                 540                 


Gly Trp Leu Leu Gln Arg Gln Ala Ala Val Ala Val Ala Ala Leu Asp 
545                 550                 555                 560 


Ala Gly Ala Ser Gly Glu Asp Arg Ser Phe Tyr Glu Gly Lys Ile Ala 
                565                 570                 575     


Val Ala Ser Phe Phe Ala Lys Asn Phe Leu Pro Met Leu Thr Ser Thr 
            580                 585                 590         


Arg Glu Val Ile Glu Thr Leu Asp Asn Asp Ile Met Glu Leu Asp Glu 
        595                 600                 605             


Ala Ala Phe 
    610     


<210>  3
<211>  300
<212>  DNA
<213>  Mycobacterium avium


<220>
<221>  CDS
<222>  (1)..(300)
<223>  MAP1507 gene encoding MAP1507 polypeptide

<400>  3
atg tcg ttc gtg acc aca cag ccg gag gct ctg gcc gcg gcg gcc ggg         48
Met Ser Phe Val Thr Thr Gln Pro Glu Ala Leu Ala Ala Ala Ala Gly           
1               5                   10                  15                

aac ctg cag gct atc ggg tgg acc ttg agc gcc cag aac gcg gcc gct         96
Asn Leu Gln Ala Ile Gly Trp Thr Leu Ser Ala Gln Asn Ala Ala Ala           
            20                  25                  30                    

gca gcc cca acg acg ggg gtg gta ccc gcc gct gcc gac gag gtc tcg        144
Ala Ala Pro Thr Thr Gly Val Val Pro Ala Ala Ala Asp Glu Val Ser           
        35                  40                  45                        

gcg ctg acc gca gcc cag ttc gcc gcg tac gcg cag atg tac cag gca        192
Ala Leu Thr Ala Ala Gln Phe Ala Ala Tyr Ala Gln Met Tyr Gln Ala           
    50                  55                  60                            

gtg agc gcc cag gcc gcc gcc atc cac gag gcg ttc gtc aac acc ctg        240
Val Ser Ala Gln Ala Ala Ala Ile His Glu Ala Phe Val Asn Thr Leu           
65                  70                  75                  80            

tcg acc agc tcc gtt tcc tac gcg gca acc gaa gca gcc aac gcc gcc        288
Ser Thr Ser Ser Val Ser Tyr Ala Ala Thr Glu Ala Ala Asn Ala Ala           
                85                  90                  95                

gcg gcc ctc tga                                                        300
Ala Ala Leu                                                               
                                                                          


<210>  4
<211>  99
<212>  PRT
<213>  Mycobacterium avium

<400>  4

Met Ser Phe Val Thr Thr Gln Pro Glu Ala Leu Ala Ala Ala Ala Gly 
1               5                   10                  15      


Asn Leu Gln Ala Ile Gly Trp Thr Leu Ser Ala Gln Asn Ala Ala Ala 
            20                  25                  30          


Ala Ala Pro Thr Thr Gly Val Val Pro Ala Ala Ala Asp Glu Val Ser 
        35                  40                  45              


Ala Leu Thr Ala Ala Gln Phe Ala Ala Tyr Ala Gln Met Tyr Gln Ala 
    50                  55                  60                  


Val Ser Ala Gln Ala Ala Ala Ile His Glu Ala Phe Val Asn Thr Leu 
65                  70                  75                  80  


Ser Thr Ser Ser Val Ser Tyr Ala Ala Thr Glu Ala Ala Asn Ala Ala 
                85                  90                  95      


Ala Ala Leu 
            


<210>  5
<211>  297
<212>  DNA
<213>  Mycobacterium avium


<220>
<221>  CDS
<222>  (1)..(297)
<223>  MAP1508 gene encoding MAP1508 polypeptide

<400>  5
atg gca aca cgt ttt atg act gac ccg cac gaa atg cgg gcg atg gcg         48
Met Ala Thr Arg Phe Met Thr Asp Pro His Glu Met Arg Ala Met Ala           
1               5                   10                  15                

ggc cgc ttc gag gtg cac gcc cag acc gtg gag gac gag gcc cgc aag         96
Gly Arg Phe Glu Val His Ala Gln Thr Val Glu Asp Glu Ala Arg Lys           
            20                  25                  30                    

atg tgg gcg tcg tcg atg aac atc gcc ggt gcg ggc tgg agt ggt cag        144
Met Trp Ala Ser Ser Met Asn Ile Ala Gly Ala Gly Trp Ser Gly Gln           
        35                  40                  45                        

gcc cag gcg acc tcg tac gac acg atg ggt cag gtc aac cag gcc ttc        192
Ala Gln Ala Thr Ser Tyr Asp Thr Met Gly Gln Val Asn Gln Ala Phe           
    50                  55                  60                            

cgc aac atc gtc aac atg ctg cac ggg gtg cgt gac ggg ctg atc cgc        240
Arg Asn Ile Val Asn Met Leu His Gly Val Arg Asp Gly Leu Ile Arg           
65                  70                  75                  80            

gac gcc aac aac tac gag cag caa gag cag gct tcg cag cag atc ctc        288
Asp Ala Asn Asn Tyr Glu Gln Gln Glu Gln Ala Ser Gln Gln Ile Leu           
                85                  90                  95                

agc agc tag                                                            297
Ser Ser                                                                   
                                                                          


<210>  6
<211>  98
<212>  PRT
<213>  Mycobacterium avium

<400>  6

Met Ala Thr Arg Phe Met Thr Asp Pro His Glu Met Arg Ala Met Ala 
1               5                   10                  15      


Gly Arg Phe Glu Val His Ala Gln Thr Val Glu Asp Glu Ala Arg Lys 
            20                  25                  30          


Met Trp Ala Ser Ser Met Asn Ile Ala Gly Ala Gly Trp Ser Gly Gln 
        35                  40                  45              


Ala Gln Ala Thr Ser Tyr Asp Thr Met Gly Gln Val Asn Gln Ala Phe 
    50                  55                  60                  


Arg Asn Ile Val Asn Met Leu His Gly Val Arg Asp Gly Leu Ile Arg 
65                  70                  75                  80  


Asp Ala Asn Asn Tyr Glu Gln Gln Glu Gln Ala Ser Gln Gln Ile Leu 
                85                  90                  95      


Ser Ser 
        


<210>  7
<211>  294
<212>  DNA
<213>  Mycobacterium avium


<220>
<221>  CDS
<222>  (1)..(294)
<223>  MAP3783 gene encoding MAP3783 polypeptide

<400>  7
atg agc atg ttg gac gct cac att ccg cag ttg gta gcc gca caa tcg         48
Met Ser Met Leu Asp Ala His Ile Pro Gln Leu Val Ala Ala Gln Ser           
1               5                   10                  15                

gcg ttc agc gcc aag gcg gcg ctg atg cgc agc acg atc agc cag gcc         96
Ala Phe Ser Ala Lys Ala Ala Leu Met Arg Ser Thr Ile Ser Gln Ala           
            20                  25                  30                    

gag cag gaa gcg gtc tcg gcc cag gcg ttc cac cag ggc gag tcc tcg        144
Glu Gln Glu Ala Val Ser Ala Gln Ala Phe His Gln Gly Glu Ser Ser           
        35                  40                  45                        

gcg gca ttc cag gcc gcg cac gcc cgc ttc gtg gaa gtc gcc gcc cgg        192
Ala Ala Phe Gln Ala Ala His Ala Arg Phe Val Glu Val Ala Ala Arg           
    50                  55                  60                            

gtc aac acc ctg ctg gac atc gcc cag gcc aac ctc ggc gac gcc gcc        240
Val Asn Thr Leu Leu Asp Ile Ala Gln Ala Asn Leu Gly Asp Ala Ala           
65                  70                  75                  80            

ggc acc tac gtg gcc gcc gac gcc gcc gcc gcg tcc ggc tac acc gcg        288
Gly Thr Tyr Val Ala Ala Asp Ala Ala Ala Ala Ser Gly Tyr Thr Ala           
                85                  90                  95                

ttc tga                                                                294
Phe                                                                       
                                                                          


<210>  8
<211>  97
<212>  PRT
<213>  Mycobacterium avium

<400>  8

Met Ser Met Leu Asp Ala His Ile Pro Gln Leu Val Ala Ala Gln Ser 
1               5                   10                  15      


Ala Phe Ser Ala Lys Ala Ala Leu Met Arg Ser Thr Ile Ser Gln Ala 
            20                  25                  30          


Glu Gln Glu Ala Val Ser Ala Gln Ala Phe His Gln Gly Glu Ser Ser 
        35                  40                  45              


Ala Ala Phe Gln Ala Ala His Ala Arg Phe Val Glu Val Ala Ala Arg 
    50                  55                  60                  


Val Asn Thr Leu Leu Asp Ile Ala Gln Ala Asn Leu Gly Asp Ala Ala 
65                  70                  75                  80  


Gly Thr Tyr Val Ala Ala Asp Ala Ala Ala Ala Ser Gly Tyr Thr Ala 
                85                  90                  95      


Phe 
    


<210>  9
<211>  291
<212>  DNA
<213>  Mycobacterium avium


<220>
<221>  CDS
<222>  (1)..(291)
<223>  MAP3784 gene encoding MAP3784 polypeptide

<400>  9
atg tca cag atc atg tac aac tac ccg gcg atg ctg agc cac gcc gcc         48
Met Ser Gln Ile Met Tyr Asn Tyr Pro Ala Met Leu Ser His Ala Ala           
1               5                   10                  15                

gac atg tcg ggc tat gcc ggc acg atg cag ggc ctg ggc gcc gac atc         96
Asp Met Ser Gly Tyr Ala Gly Thr Met Gln Gly Leu Gly Ala Asp Ile           
            20                  25                  30                    

gcc agc gag cag gcc acc ctg tcc aac gcc tgg cag ggt gac acc ggg        144
Ala Ser Glu Gln Ala Thr Leu Ser Asn Ala Trp Gln Gly Asp Thr Gly           
        35                  40                  45                        

atg acc tac cag gtg tgg cag gcg cag tgg aac gag gcc atg gag agc        192
Met Thr Tyr Gln Val Trp Gln Ala Gln Trp Asn Glu Ala Met Glu Ser           
    50                  55                  60                            

ctg gtg cgc gcc tac cag tcg atg gcc agc acc cac gag gcc aac acc        240
Leu Val Arg Ala Tyr Gln Ser Met Ala Ser Thr His Glu Ala Asn Thr           
65                  70                  75                  80            

atg tcc atg ttg gcc cgc gac cag gcc gaa gcc gcc aaa tgg ggc ggc        288
Met Ser Met Leu Ala Arg Asp Gln Ala Glu Ala Ala Lys Trp Gly Gly           
                85                  90                  95                

tag                                                                    291


<210>  10
<211>  96
<212>  PRT
<213>  Mycobacterium avium

<400>  10

Met Ser Gln Ile Met Tyr Asn Tyr Pro Ala Met Leu Ser His Ala Ala 
1               5                   10                  15      


Asp Met Ser Gly Tyr Ala Gly Thr Met Gln Gly Leu Gly Ala Asp Ile 
            20                  25                  30          


Ala Ser Glu Gln Ala Thr Leu Ser Asn Ala Trp Gln Gly Asp Thr Gly 
        35                  40                  45              


Met Thr Tyr Gln Val Trp Gln Ala Gln Trp Asn Glu Ala Met Glu Ser 
    50                  55                  60                  


Leu Val Arg Ala Tyr Gln Ser Met Ala Ser Thr His Glu Ala Asn Thr 
65                  70                  75                  80  


Met Ser Met Leu Ala Arg Asp Gln Ala Glu Ala Ala Lys Trp Gly Gly 
                85                  90                  95      


<210>  11
<211>  1266
<212>  DNA
<213>  Mycobacterium avium


<220>
<221>  CDS
<222>  (1)..(1266)
<223>  MAP fusion gene encoding MAP1507-MAP1508-MAP3783-MAP3784 fusion 
       polypeptide Residues 2-7 His tag; residues 1,8-105: MAP1507; 
       114-211: MAP1508; 220-316:MAP3783; 326-421: MAP3784. Residues 
       106-113/212-219/317-325: spacers

<400>  11
atg cac cac cac cac cat cat tcg ttc gtc acc acc cag cca gag gcg         48
Met His His His His His His Ser Phe Val Thr Thr Gln Pro Glu Ala           
1               5                   10                  15                

ctg gct gcg gca gcg ggt aat ctg cag gca att ggc tgg acg ttg agc         96
Leu Ala Ala Ala Ala Gly Asn Leu Gln Ala Ile Gly Trp Thr Leu Ser           
            20                  25                  30                    

gcg caa aat gct gcg gct gca gcg ccg acc acg ggt gtg gtc ccg gca        144
Ala Gln Asn Ala Ala Ala Ala Ala Pro Thr Thr Gly Val Val Pro Ala           
        35                  40                  45                        

gcg gcg gac gag gtt tct gcg ctg acc gcc gca cag ttt gct gcg tac        192
Ala Ala Asp Glu Val Ser Ala Leu Thr Ala Ala Gln Phe Ala Ala Tyr           
    50                  55                  60                            

gcg cag atg tat caa gca gtt agc gcg caa gca gcc gca atc cac gaa        240
Ala Gln Met Tyr Gln Ala Val Ser Ala Gln Ala Ala Ala Ile His Glu           
65                  70                  75                  80            

gcc ttt gtt aac acc ctg agc acc agc agc gtc agc tac gct gcg acc        288
Ala Phe Val Asn Thr Leu Ser Thr Ser Ser Val Ser Tyr Ala Ala Thr           
                85                  90                  95                

gag gcg gcc aat gcg gca gcc gcc ctg tct gcc tgc tac tgt gaa ctg        336
Glu Ala Ala Asn Ala Ala Ala Ala Leu Ser Ala Cys Tyr Cys Glu Leu           
            100                 105                 110                   

agc atg gcg acg cgc ttc atg act gat ccg cac gag atg cgt gcg atg        384
Ser Met Ala Thr Arg Phe Met Thr Asp Pro His Glu Met Arg Ala Met           
        115                 120                 125                       

gcg ggt cgc ttc gaa gtc cac gcc cag acg gtg gag gac gag gcc cgc        432
Ala Gly Arg Phe Glu Val His Ala Gln Thr Val Glu Asp Glu Ala Arg           
    130                 135                 140                           

aag atg tgg gct tcc agc atg aac att gcg ggt gcg ggt tgg agc ggc        480
Lys Met Trp Ala Ser Ser Met Asn Ile Ala Gly Ala Gly Trp Ser Gly           
145                 150                 155                 160           

cag gcg cag gca act agc tac gac acc atg ggt cag gta aat caa gcg        528
Gln Ala Gln Ala Thr Ser Tyr Asp Thr Met Gly Gln Val Asn Gln Ala           
                165                 170                 175               

ttc cgc aat atc gtc aac atg ctg cac ggc gtt cgt gac ggt ctg att        576
Phe Arg Asn Ile Val Asn Met Leu His Gly Val Arg Asp Gly Leu Ile           
            180                 185                 190                   

cgt gat gca aac aac tat gaa caa caa gag caa gca tct caa cag atc        624
Arg Asp Ala Asn Asn Tyr Glu Gln Gln Glu Gln Ala Ser Gln Gln Ile           
        195                 200                 205                       

ctg tcc agc ggt ggt agc ggt ggc ggt agc ggc atg agc atg ttg gat        672
Leu Ser Ser Gly Gly Ser Gly Gly Gly Ser Gly Met Ser Met Leu Asp           
    210                 215                 220                           

gcc cac atc ccg caa ctg gtg gct gcg cag agc gcg ttt agc gcg aaa        720
Ala His Ile Pro Gln Leu Val Ala Ala Gln Ser Ala Phe Ser Ala Lys           
225                 230                 235                 240           

gcg gcg ttg atg cgt agc acc att agc cag gcg gag cag gaa gca gtg        768
Ala Ala Leu Met Arg Ser Thr Ile Ser Gln Ala Glu Gln Glu Ala Val           
                245                 250                 255               

tcc gca cag gca ttc cat caa ggt gag agc agc gcg gca ttc caa gcg        816
Ser Ala Gln Ala Phe His Gln Gly Glu Ser Ser Ala Ala Phe Gln Ala           
            260                 265                 270                   

gcg cat gcc cgt ttt gtt gaa gtg gca gcc cgc gtg aac acc ctg ctg        864
Ala His Ala Arg Phe Val Glu Val Ala Ala Arg Val Asn Thr Leu Leu           
        275                 280                 285                       

gat att gca cag gcg aat ttg ggc gac gcg gca ggt acg tat gtg gcg        912
Asp Ile Ala Gln Ala Asn Leu Gly Asp Ala Ala Gly Thr Tyr Val Ala           
    290                 295                 300                           

gca gat gcg gct gcg gcg tcc ggt tac acg gct ttt ggt ctg gtt ccg        960
Ala Asp Ala Ala Ala Ala Ser Gly Tyr Thr Ala Phe Gly Leu Val Pro           
305                 310                 315                 320           

cgt ggc tct acc ggt atg agc caa atc atg tac aat tat ccg gcg atg       1008
Arg Gly Ser Thr Gly Met Ser Gln Ile Met Tyr Asn Tyr Pro Ala Met           
                325                 330                 335               

ctg agc cat gcc gcg gac atg agc ggc tac gcc ggt acg atg caa ggc       1056
Leu Ser His Ala Ala Asp Met Ser Gly Tyr Ala Gly Thr Met Gln Gly           
            340                 345                 350                   

ctg ggt gca gat atc gca agc gaa cag gcg acg ctg tcc aac gcc tgg       1104
Leu Gly Ala Asp Ile Ala Ser Glu Gln Ala Thr Leu Ser Asn Ala Trp           
        355                 360                 365                       

cag ggc gac acc ggc atg acc tat caa gtc tgg cag gcg cag tgg aat       1152
Gln Gly Asp Thr Gly Met Thr Tyr Gln Val Trp Gln Ala Gln Trp Asn           
    370                 375                 380                           

gaa gca atg gag tcg ctg gtt cgt gcc tac cag agc atg gct agc acg       1200
Glu Ala Met Glu Ser Leu Val Arg Ala Tyr Gln Ser Met Ala Ser Thr           
385                 390                 395                 400           

cat gag gcc aac acc atg tcc atg ctg gca cgt gac caa gcg gaa gcc       1248
His Glu Ala Asn Thr Met Ser Met Leu Ala Arg Asp Gln Ala Glu Ala           
                405                 410                 415               

gcg aaa tgg ggc ggt taa                                               1266
Ala Lys Trp Gly Gly                                                       
            420                                                           


<210>  12
<211>  421
<212>  PRT
<213>  Mycobacterium avium

<400>  12

Met His His His His His His Ser Phe Val Thr Thr Gln Pro Glu Ala 
1               5                   10                  15      


Leu Ala Ala Ala Ala Gly Asn Leu Gln Ala Ile Gly Trp Thr Leu Ser 
            20                  25                  30          


Ala Gln Asn Ala Ala Ala Ala Ala Pro Thr Thr Gly Val Val Pro Ala 
        35                  40                  45              


Ala Ala Asp Glu Val Ser Ala Leu Thr Ala Ala Gln Phe Ala Ala Tyr 
    50                  55                  60                  


Ala Gln Met Tyr Gln Ala Val Ser Ala Gln Ala Ala Ala Ile His Glu 
65                  70                  75                  80  


Ala Phe Val Asn Thr Leu Ser Thr Ser Ser Val Ser Tyr Ala Ala Thr 
                85                  90                  95      


Glu Ala Ala Asn Ala Ala Ala Ala Leu Ser Ala Cys Tyr Cys Glu Leu 
            100                 105                 110         


Ser Met Ala Thr Arg Phe Met Thr Asp Pro His Glu Met Arg Ala Met 
        115                 120                 125             


Ala Gly Arg Phe Glu Val His Ala Gln Thr Val Glu Asp Glu Ala Arg 
    130                 135                 140                 


Lys Met Trp Ala Ser Ser Met Asn Ile Ala Gly Ala Gly Trp Ser Gly 
145                 150                 155                 160 


Gln Ala Gln Ala Thr Ser Tyr Asp Thr Met Gly Gln Val Asn Gln Ala 
                165                 170                 175     


Phe Arg Asn Ile Val Asn Met Leu His Gly Val Arg Asp Gly Leu Ile 
            180                 185                 190         


Arg Asp Ala Asn Asn Tyr Glu Gln Gln Glu Gln Ala Ser Gln Gln Ile 
        195                 200                 205             


Leu Ser Ser Gly Gly Ser Gly Gly Gly Ser Gly Met Ser Met Leu Asp 
    210                 215                 220                 


Ala His Ile Pro Gln Leu Val Ala Ala Gln Ser Ala Phe Ser Ala Lys 
225                 230                 235                 240 


Ala Ala Leu Met Arg Ser Thr Ile Ser Gln Ala Glu Gln Glu Ala Val 
                245                 250                 255     


Ser Ala Gln Ala Phe His Gln Gly Glu Ser Ser Ala Ala Phe Gln Ala 
            260                 265                 270         


Ala His Ala Arg Phe Val Glu Val Ala Ala Arg Val Asn Thr Leu Leu 
        275                 280                 285             


Asp Ile Ala Gln Ala Asn Leu Gly Asp Ala Ala Gly Thr Tyr Val Ala 
    290                 295                 300                 


Ala Asp Ala Ala Ala Ala Ser Gly Tyr Thr Ala Phe Gly Leu Val Pro 
305                 310                 315                 320 


Arg Gly Ser Thr Gly Met Ser Gln Ile Met Tyr Asn Tyr Pro Ala Met 
                325                 330                 335     


Leu Ser His Ala Ala Asp Met Ser Gly Tyr Ala Gly Thr Met Gln Gly 
            340                 345                 350         


Leu Gly Ala Asp Ile Ala Ser Glu Gln Ala Thr Leu Ser Asn Ala Trp 
        355                 360                 365             


Gln Gly Asp Thr Gly Met Thr Tyr Gln Val Trp Gln Ala Gln Trp Asn 
    370                 375                 380                 


Glu Ala Met Glu Ser Leu Val Arg Ala Tyr Gln Ser Met Ala Ser Thr 
385                 390                 395                 400 


His Glu Ala Asn Thr Met Ser Met Leu Ala Arg Asp Gln Ala Glu Ala 
                405                 410                 415     


Ala Lys Trp Gly Gly 
            420     


<210>  13
<211>  1854
<212>  DNA
<213>  Mycobacterium avium


<220>
<221>  CDS
<222>  (1)..(1854)
<223>  rMAP3694 gene encoding His-tagged MAP3694 polypeptide

<400>  13
atg cat cac cac cac cat cac agc cac tac aaa agc aac gtc cgt gat         48
Met His His His His His His Ser His Tyr Lys Ser Asn Val Arg Asp           
1               5                   10                  15                

cag gag ttc aac ttg ttt gag gtt ctg ggt gtg gac aaa gct ctg ggt         96
Gln Glu Phe Asn Leu Phe Glu Val Leu Gly Val Asp Lys Ala Leu Gly           
            20                  25                  30                    

cag ggt gag ttt agc gac ctg gac gct gat acg gca cgc gag atg ttg        144
Gln Gly Glu Phe Ser Asp Leu Asp Ala Asp Thr Ala Arg Glu Met Leu           
        35                  40                  45                        

aat gaa att tcc cgt ctg gcg gag ggc ccg att gca gat agc ttc gtc        192
Asn Glu Ile Ser Arg Leu Ala Glu Gly Pro Ile Ala Asp Ser Phe Val           
    50                  55                  60                            

gag ggt gac cgt aat ccg ccg gtt ttt gac ccg aaa acc cat agc gtc        240
Glu Gly Asp Arg Asn Pro Pro Val Phe Asp Pro Lys Thr His Ser Val           
65                  70                  75                  80            

acg ctg ccg gaa agc ttt aag aaa tcg gtg cat gcc gtg att gag gca        288
Thr Leu Pro Glu Ser Phe Lys Lys Ser Val His Ala Val Ile Glu Ala           
                85                  90                  95                

ggt tgg gac aag gtg ggt atc gat gag gcg ttg ggt ggc gtt gca atg        336
Gly Trp Asp Lys Val Gly Ile Asp Glu Ala Leu Gly Gly Val Ala Met           
            100                 105                 110                   

cca aaa gct ctg ctg tgg gcc ctg cat gaa cac atc ctg ggc gcc aac        384
Pro Lys Ala Leu Leu Trp Ala Leu His Glu His Ile Leu Gly Ala Asn           
        115                 120                 125                       

ccg gca gtc tgg atg tac gcc ggt ggc gca ggc ttt gct aac atc ctg        432
Pro Ala Val Trp Met Tyr Ala Gly Gly Ala Gly Phe Ala Asn Ile Leu           
    130                 135                 140                           

tac cat ctg ggt acc gat gag caa aag aag tgg gct gtt atg gcg gct        480
Tyr His Leu Gly Thr Asp Glu Gln Lys Lys Trp Ala Val Met Ala Ala           
145                 150                 155                 160           

gaa cgc ggc tgg ggc agc acc atg gtt ctg acc gag ccg gat gcg ggc        528
Glu Arg Gly Trp Gly Ser Thr Met Val Leu Thr Glu Pro Asp Ala Gly           
                165                 170                 175               

agc gac gtg ggc gca ggt cgt acg aaa gcc gtg aaa caa gat gac ggt        576
Ser Asp Val Gly Ala Gly Arg Thr Lys Ala Val Lys Gln Asp Asp Gly           
            180                 185                 190                   

tct tgg cac atc gat ggt gtt aaa cgt ttc atc acc agc gcc gat tcc        624
Ser Trp His Ile Asp Gly Val Lys Arg Phe Ile Thr Ser Ala Asp Ser           
        195                 200                 205                       

gac gac ctg ttc gag aat atc ttc cat ctg gtc ctg gca cgc cct gag        672
Asp Asp Leu Phe Glu Asn Ile Phe His Leu Val Leu Ala Arg Pro Glu           
    210                 215                 220                           

ggt gcg ggt ccg ggc acg aaa ggc ctg tcg ctg ttt ttc gtt ccg aag        720
Gly Ala Gly Pro Gly Thr Lys Gly Leu Ser Leu Phe Phe Val Pro Lys           
225                 230                 235                 240           

ttt ctg ttc gat ttt gaa acg ggc gaa ctg ggt gag cgc aac ggt gtg        768
Phe Leu Phe Asp Phe Glu Thr Gly Glu Leu Gly Glu Arg Asn Gly Val           
                245                 250                 255               

ttt gtt acg aat gtc gaa cac aaa atg ggt ctg aaa gtg agc gcg acc        816
Phe Val Thr Asn Val Glu His Lys Met Gly Leu Lys Val Ser Ala Thr           
            260                 265                 270                   

tgc gag ttg agc ttc ggc caa cac gac gtt ccg gcg aaa ggc tgg ctg        864
Cys Glu Leu Ser Phe Gly Gln His Asp Val Pro Ala Lys Gly Trp Leu           
        275                 280                 285                       

gtc ggt gaa gtg cac aac ggt atc gcg cag atg ttc gag gtt atc gag        912
Val Gly Glu Val His Asn Gly Ile Ala Gln Met Phe Glu Val Ile Glu           
    290                 295                 300                           

caa gca cgt atg atg gtg ggt acg aag gcg att gct acc ctg tcc acc        960
Gln Ala Arg Met Met Val Gly Thr Lys Ala Ile Ala Thr Leu Ser Thr           
305                 310                 315                 320           

ggc tat ttg aat gcc ctg gaa tat gcg aag tcc cgt gtg caa ggc gca       1008
Gly Tyr Leu Asn Ala Leu Glu Tyr Ala Lys Ser Arg Val Gln Gly Ala           
                325                 330                 335               

gac atg acc cag atg acc gac aaa acc gca ccg cgt gta acc atc acc       1056
Asp Met Thr Gln Met Thr Asp Lys Thr Ala Pro Arg Val Thr Ile Thr           
            340                 345                 350                   

cat cac ccg gat gtc cgc cgt agc ttg atg acg cag aag gcg tac gca       1104
His His Pro Asp Val Arg Arg Ser Leu Met Thr Gln Lys Ala Tyr Ala           
        355                 360                 365                       

gag ggc ctg cgt gcg ctg tac ctg ttc acc tcc acc tac cag gat gcg       1152
Glu Gly Leu Arg Ala Leu Tyr Leu Phe Thr Ser Thr Tyr Gln Asp Ala           
    370                 375                 380                           

gcg gtt gcg gaa gcg ttg tat ggc gtc gat gcg gaa ctg gcg gtt aag       1200
Ala Val Ala Glu Ala Leu Tyr Gly Val Asp Ala Glu Leu Ala Val Lys           
385                 390                 395                 400           

gtt aat gac ctg atg ctg ccg gtg gtg aaa ggt gtc ggc agc gag cag       1248
Val Asn Asp Leu Met Leu Pro Val Val Lys Gly Val Gly Ser Glu Gln           
                405                 410                 415               

gcc tat gca aag ctg acc gag agc ctg caa acc ttt ggt ggc agc ggt       1296
Ala Tyr Ala Lys Leu Thr Glu Ser Leu Gln Thr Phe Gly Gly Ser Gly           
            420                 425                 430                   

ttt ctg cag gat tac ccg att gaa cag tat att cgt gac tct aaa atc       1344
Phe Leu Gln Asp Tyr Pro Ile Glu Gln Tyr Ile Arg Asp Ser Lys Ile           
        435                 440                 445                       

gat agc ctg tac gaa ggt acg acg gcg att cag gcc cag gac ttt ttc       1392
Asp Ser Leu Tyr Glu Gly Thr Thr Ala Ile Gln Ala Gln Asp Phe Phe           
    450                 455                 460                           

ttt cgc aag att gtg cgt gac aag ggc gtc gcg ttg gcg cac gtg agc       1440
Phe Arg Lys Ile Val Arg Asp Lys Gly Val Ala Leu Ala His Val Ser           
465                 470                 475                 480           

gag caa att cag aaa ttc gtg gac agc gaa agc ggt aac ggt cgt ctg       1488
Glu Gln Ile Gln Lys Phe Val Asp Ser Glu Ser Gly Asn Gly Arg Leu           
                485                 490                 495               

aag agc gaa cgc gag ctg ctg gca aaa gcc ctg gct gac gtg caa ggt       1536
Lys Ser Glu Arg Glu Leu Leu Ala Lys Ala Leu Ala Asp Val Gln Gly           
            500                 505                 510                   

atg gcg act gcg ctg acg ggc tac ctg atg gct gcg caa gag gat gtt       1584
Met Ala Thr Ala Leu Thr Gly Tyr Leu Met Ala Ala Gln Glu Asp Val           
        515                 520                 525                       

act agc ctg tat aag gtc ggt ctg ggt agc gtc cgc ttc ctg atg tcc       1632
Thr Ser Leu Tyr Lys Val Gly Leu Gly Ser Val Arg Phe Leu Met Ser           
    530                 535                 540                           

gtt ggt gac ttg atc att ggt tgg ctg ctg cag cgt caa gcc gca gtc       1680
Val Gly Asp Leu Ile Ile Gly Trp Leu Leu Gln Arg Gln Ala Ala Val           
545                 550                 555                 560           

gcg gtt gcc gct ttg gat gcg ggt gcg agc ggt gaa gat cgc tct ttc       1728
Ala Val Ala Ala Leu Asp Ala Gly Ala Ser Gly Glu Asp Arg Ser Phe           
                565                 570                 575               

tat gag ggt aag att gca gtt gcg agc ttc ttt gcg aag aac ttc ctg       1776
Tyr Glu Gly Lys Ile Ala Val Ala Ser Phe Phe Ala Lys Asn Phe Leu           
            580                 585                 590                   

cca atg ctg acc agc acc cgt gaa gtg att gaa acc ctg gac aat gac       1824
Pro Met Leu Thr Ser Thr Arg Glu Val Ile Glu Thr Leu Asp Asn Asp           
        595                 600                 605                       

atc atg gaa ttg gat gag gcc gcg ttc taa                               1854
Ile Met Glu Leu Asp Glu Ala Ala Phe                                       
    610                 615                                               


<210>  14
<211>  617
<212>  PRT
<213>  Mycobacterium avium

<400>  14

Met His His His His His His Ser His Tyr Lys Ser Asn Val Arg Asp 
1               5                   10                  15      


Gln Glu Phe Asn Leu Phe Glu Val Leu Gly Val Asp Lys Ala Leu Gly 
            20                  25                  30          


Gln Gly Glu Phe Ser Asp Leu Asp Ala Asp Thr Ala Arg Glu Met Leu 
        35                  40                  45              


Asn Glu Ile Ser Arg Leu Ala Glu Gly Pro Ile Ala Asp Ser Phe Val 
    50                  55                  60                  


Glu Gly Asp Arg Asn Pro Pro Val Phe Asp Pro Lys Thr His Ser Val 
65                  70                  75                  80  


Thr Leu Pro Glu Ser Phe Lys Lys Ser Val His Ala Val Ile Glu Ala 
                85                  90                  95      


Gly Trp Asp Lys Val Gly Ile Asp Glu Ala Leu Gly Gly Val Ala Met 
            100                 105                 110         


Pro Lys Ala Leu Leu Trp Ala Leu His Glu His Ile Leu Gly Ala Asn 
        115                 120                 125             


Pro Ala Val Trp Met Tyr Ala Gly Gly Ala Gly Phe Ala Asn Ile Leu 
    130                 135                 140                 


Tyr His Leu Gly Thr Asp Glu Gln Lys Lys Trp Ala Val Met Ala Ala 
145                 150                 155                 160 


Glu Arg Gly Trp Gly Ser Thr Met Val Leu Thr Glu Pro Asp Ala Gly 
                165                 170                 175     


Ser Asp Val Gly Ala Gly Arg Thr Lys Ala Val Lys Gln Asp Asp Gly 
            180                 185                 190         


Ser Trp His Ile Asp Gly Val Lys Arg Phe Ile Thr Ser Ala Asp Ser 
        195                 200                 205             


Asp Asp Leu Phe Glu Asn Ile Phe His Leu Val Leu Ala Arg Pro Glu 
    210                 215                 220                 


Gly Ala Gly Pro Gly Thr Lys Gly Leu Ser Leu Phe Phe Val Pro Lys 
225                 230                 235                 240 


Phe Leu Phe Asp Phe Glu Thr Gly Glu Leu Gly Glu Arg Asn Gly Val 
                245                 250                 255     


Phe Val Thr Asn Val Glu His Lys Met Gly Leu Lys Val Ser Ala Thr 
            260                 265                 270         


Cys Glu Leu Ser Phe Gly Gln His Asp Val Pro Ala Lys Gly Trp Leu 
        275                 280                 285             


Val Gly Glu Val His Asn Gly Ile Ala Gln Met Phe Glu Val Ile Glu 
    290                 295                 300                 


Gln Ala Arg Met Met Val Gly Thr Lys Ala Ile Ala Thr Leu Ser Thr 
305                 310                 315                 320 


Gly Tyr Leu Asn Ala Leu Glu Tyr Ala Lys Ser Arg Val Gln Gly Ala 
                325                 330                 335     


Asp Met Thr Gln Met Thr Asp Lys Thr Ala Pro Arg Val Thr Ile Thr 
            340                 345                 350         


His His Pro Asp Val Arg Arg Ser Leu Met Thr Gln Lys Ala Tyr Ala 
        355                 360                 365             


Glu Gly Leu Arg Ala Leu Tyr Leu Phe Thr Ser Thr Tyr Gln Asp Ala 
    370                 375                 380                 


Ala Val Ala Glu Ala Leu Tyr Gly Val Asp Ala Glu Leu Ala Val Lys 
385                 390                 395                 400 


Val Asn Asp Leu Met Leu Pro Val Val Lys Gly Val Gly Ser Glu Gln 
                405                 410                 415     


Ala Tyr Ala Lys Leu Thr Glu Ser Leu Gln Thr Phe Gly Gly Ser Gly 
            420                 425                 430         


Phe Leu Gln Asp Tyr Pro Ile Glu Gln Tyr Ile Arg Asp Ser Lys Ile 
        435                 440                 445             


Asp Ser Leu Tyr Glu Gly Thr Thr Ala Ile Gln Ala Gln Asp Phe Phe 
    450                 455                 460                 


Phe Arg Lys Ile Val Arg Asp Lys Gly Val Ala Leu Ala His Val Ser 
465                 470                 475                 480 


Glu Gln Ile Gln Lys Phe Val Asp Ser Glu Ser Gly Asn Gly Arg Leu 
                485                 490                 495     


Lys Ser Glu Arg Glu Leu Leu Ala Lys Ala Leu Ala Asp Val Gln Gly 
            500                 505                 510         


Met Ala Thr Ala Leu Thr Gly Tyr Leu Met Ala Ala Gln Glu Asp Val 
        515                 520                 525             


Thr Ser Leu Tyr Lys Val Gly Leu Gly Ser Val Arg Phe Leu Met Ser 
    530                 535                 540                 


Val Gly Asp Leu Ile Ile Gly Trp Leu Leu Gln Arg Gln Ala Ala Val 
545                 550                 555                 560 


Ala Val Ala Ala Leu Asp Ala Gly Ala Ser Gly Glu Asp Arg Ser Phe 
                565                 570                 575     


Tyr Glu Gly Lys Ile Ala Val Ala Ser Phe Phe Ala Lys Asn Phe Leu 
            580                 585                 590         


Pro Met Leu Thr Ser Thr Arg Glu Val Ile Glu Thr Leu Asp Asn Asp 
        595                 600                 605             


Ile Met Glu Leu Asp Glu Ala Ala Phe 
    610                 615         


<210>  15
<211>  20
<212>  DNA
<213>  Mycobacterium avium


<220>
<221>  primer_bind
<222>  (1)..(20)
<223>  qPCR IS900 forward primer

<400>  15
ggcaagaccg acgccaaaga                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Mycobacterium avium


<220>
<221>  primer_bind
<222>  (1)..(20)
<223>  qPCR IS900 reverse primer

<400>  16
gggtccgatc agccaccaga                                                   20


<210>  17
<211>  3105
<212>  DNA
<213>  Mycobacterium avium


<220>
<221>  CDS
<222>  (1)..(3099)
<223>  MAP fusion gene encoding MAP1507-MAP1508-MAP3783-MAP3784-map3694C
       fusion polypeptide Residues 2-7 His tag; residues 1,8-105: 
       MAP1507; 114-211: MAP1508; 220-316:MAP3783; 326-421: MAP3784; 
       422- 1032; Residues 106-113/212-219/317-325: spacers

<400>  17
atg cat cat cac cat cac cac tct ttc gtg acc acc caa cca gag gcc         48
Met His His His His His His Ser Phe Val Thr Thr Gln Pro Glu Ala           
1               5                   10                  15                

ctg gct gcc gca gcg ggc aat ctg cag gcg att ggc tgg acc ctg agc         96
Leu Ala Ala Ala Ala Gly Asn Leu Gln Ala Ile Gly Trp Thr Leu Ser           
            20                  25                  30                    

gcg cag aac gcg gcg gct gca gcg cca acc acg ggt gtg gtg ccg gca        144
Ala Gln Asn Ala Ala Ala Ala Ala Pro Thr Thr Gly Val Val Pro Ala           
        35                  40                  45                        

gcg gcc gat gaa gtg tcc gcg ctg acg gcg gca cag ttt gcg gcc tat        192
Ala Ala Asp Glu Val Ser Ala Leu Thr Ala Ala Gln Phe Ala Ala Tyr           
    50                  55                  60                            

gcg cag atg tat cag gca gtg agc gcg caa gcg gca gcg att cat gaa        240
Ala Gln Met Tyr Gln Ala Val Ser Ala Gln Ala Ala Ala Ile His Glu           
65                  70                  75                  80            

gcg ttc gtc aac acc ctg agc acc agc tct gtt tcc tat gcc gcg acc        288
Ala Phe Val Asn Thr Leu Ser Thr Ser Ser Val Ser Tyr Ala Ala Thr           
                85                  90                  95                

gaa gca gcc aat gcc gca gct gcg ctg tct gcg tgc tac tgc gag ctg        336
Glu Ala Ala Asn Ala Ala Ala Ala Leu Ser Ala Cys Tyr Cys Glu Leu           
            100                 105                 110                   

tcg atg gcg acg cgt ttt atg acc gat ccg cat gaa atg cgc gct atg        384
Ser Met Ala Thr Arg Phe Met Thr Asp Pro His Glu Met Arg Ala Met           
        115                 120                 125                       

gcc ggt cgc ttc gag gtc cac gcc caa acc gtt gaa gat gaa gcc cgc        432
Ala Gly Arg Phe Glu Val His Ala Gln Thr Val Glu Asp Glu Ala Arg           
    130                 135                 140                           

aag atg tgg gcg agc agc atg aac att gct ggt gcc ggc tgg tca ggt        480
Lys Met Trp Ala Ser Ser Met Asn Ile Ala Gly Ala Gly Trp Ser Gly           
145                 150                 155                 160           

caa gcc caa gct acg agc tac gac acc atg ggt caa gtt aac cag gcg        528
Gln Ala Gln Ala Thr Ser Tyr Asp Thr Met Gly Gln Val Asn Gln Ala           
                165                 170                 175               

ttc cgc aat atc gtg aat atg ctg cat ggt gtt cgt gac ggt ctg att        576
Phe Arg Asn Ile Val Asn Met Leu His Gly Val Arg Asp Gly Leu Ile           
            180                 185                 190                   

cgc gac gca aat aac tac gaa cag caa gag caa gca tct cag cag att        624
Arg Asp Ala Asn Asn Tyr Glu Gln Gln Glu Gln Ala Ser Gln Gln Ile           
        195                 200                 205                       

ctg agc agc ggc ggt tcg ggt ggc ggc agc ggt atg tcg atg ctg gac        672
Leu Ser Ser Gly Gly Ser Gly Gly Gly Ser Gly Met Ser Met Leu Asp           
    210                 215                 220                           

gcg cac att ccg cag ctg gtc gcg gct cag agc gca ttt agc gca aag        720
Ala His Ile Pro Gln Leu Val Ala Ala Gln Ser Ala Phe Ser Ala Lys           
225                 230                 235                 240           

gct gcg ctg atg cgt agc acg atc agc cag gca gaa caa gaa gcc gta        768
Ala Ala Leu Met Arg Ser Thr Ile Ser Gln Ala Glu Gln Glu Ala Val           
                245                 250                 255               

agc gca caa gcg ttc cac caa ggt gag agc tct gcg gcg ttc caa gca        816
Ser Ala Gln Ala Phe His Gln Gly Glu Ser Ser Ala Ala Phe Gln Ala           
            260                 265                 270                   

gca cac gct cgt ttt gtt gaa gtc gca gca cgt gtg aac acc ctg ttg        864
Ala His Ala Arg Phe Val Glu Val Ala Ala Arg Val Asn Thr Leu Leu           
        275                 280                 285                       

gat att gcg cag gca aac ttg ggc gat gct gcg ggc acc tat gtt gcg        912
Asp Ile Ala Gln Ala Asn Leu Gly Asp Ala Ala Gly Thr Tyr Val Ala           
    290                 295                 300                           

gca gac gcc gct gcg gcg agc ggc tat acc gcg ttc ggt ctg gtc ccg        960
Ala Asp Ala Ala Ala Ala Ser Gly Tyr Thr Ala Phe Gly Leu Val Pro           
305                 310                 315                 320           

cgt ggt agc acc ggt atg agc cag att atg tac aat tac ccg gcg atg       1008
Arg Gly Ser Thr Gly Met Ser Gln Ile Met Tyr Asn Tyr Pro Ala Met           
                325                 330                 335               

ttg tcc cat gct gca gat atg agc ggc tac gcg ggt acg atg caa ggt       1056
Leu Ser His Ala Ala Asp Met Ser Gly Tyr Ala Gly Thr Met Gln Gly           
            340                 345                 350                   

ctg ggt gca gac atc gct agc gaa caa gcc acc ctg tcc aat gcg tgg       1104
Leu Gly Ala Asp Ile Ala Ser Glu Gln Ala Thr Leu Ser Asn Ala Trp           
        355                 360                 365                       

cag ggt gat acg ggt atg acc tat cag gtg tgg cag gcg cag tgg aac       1152
Gln Gly Asp Thr Gly Met Thr Tyr Gln Val Trp Gln Ala Gln Trp Asn           
    370                 375                 380                           

gaa gcg atg gag agc ctg gtg cgt gcg tac caa agc atg gcg tca acg       1200
Glu Ala Met Glu Ser Leu Val Arg Ala Tyr Gln Ser Met Ala Ser Thr           
385                 390                 395                 400           

cac gaa gcg aac act atg agc atg ttg gct cgc gac cag gct gag gca       1248
His Glu Ala Asn Thr Met Ser Met Leu Ala Arg Asp Gln Ala Glu Ala           
                405                 410                 415               

gcg aag tgg ggt ggc atg agc cat tac aag tct aac gtt cgt gat caa       1296
Ala Lys Trp Gly Gly Met Ser His Tyr Lys Ser Asn Val Arg Asp Gln           
            420                 425                 430                   

gag ttc aat ttg ttc gag gtt ctg ggt gtc gat aag gca ctg ggt cag       1344
Glu Phe Asn Leu Phe Glu Val Leu Gly Val Asp Lys Ala Leu Gly Gln           
        435                 440                 445                       

ggt gag ttc agc gat ctg gat gcc gac act gcg cgc gag atg ctt aac       1392
Gly Glu Phe Ser Asp Leu Asp Ala Asp Thr Ala Arg Glu Met Leu Asn           
    450                 455                 460                           

gaa atc agc cgt ctg gca gag ggt cca att gcc gac tcg ttt gtt gag       1440
Glu Ile Ser Arg Leu Ala Glu Gly Pro Ile Ala Asp Ser Phe Val Glu           
465                 470                 475                 480           

ggt gac cgt aat ccg ccg gtt ttc gac ccg aaa acc cat tcc gtc acc       1488
Gly Asp Arg Asn Pro Pro Val Phe Asp Pro Lys Thr His Ser Val Thr           
                485                 490                 495               

ctg ccg gaa agc ttt aag aaa agc gtt cat gcg gtt atc gag gca ggc       1536
Leu Pro Glu Ser Phe Lys Lys Ser Val His Ala Val Ile Glu Ala Gly           
            500                 505                 510                   

tgg gat aag gtt ggc atc gac gaa gcg ctg ggc ggt gtg gcg atg ccg       1584
Trp Asp Lys Val Gly Ile Asp Glu Ala Leu Gly Gly Val Ala Met Pro           
        515                 520                 525                       

aaa gcg ctg ctc tgg gcg ctg cat gag cac atc ctg ggc gca aac ccg       1632
Lys Ala Leu Leu Trp Ala Leu His Glu His Ile Leu Gly Ala Asn Pro           
    530                 535                 540                           

gca gtc tgg atg tat gcc ggt ggt gcg ggt ttt gcg aat atc ctg tac       1680
Ala Val Trp Met Tyr Ala Gly Gly Ala Gly Phe Ala Asn Ile Leu Tyr           
545                 550                 555                 560           

cac ctg ggt acc gac gag cag aaa aag tgg gcg gtt atg gca gcg gag       1728
His Leu Gly Thr Asp Glu Gln Lys Lys Trp Ala Val Met Ala Ala Glu           
                565                 570                 575               

cgc ggc tgg ggt tcc acg atg gtc ttg acg gag cct gac gca ggc agc       1776
Arg Gly Trp Gly Ser Thr Met Val Leu Thr Glu Pro Asp Ala Gly Ser           
            580                 585                 590                   

gac gtg ggt gcg ggt cgt acg aaa gcc gtt aag caa gat gac ggt agc       1824
Asp Val Gly Ala Gly Arg Thr Lys Ala Val Lys Gln Asp Asp Gly Ser           
        595                 600                 605                       

tgg cac atc gat ggc gtc aaa cgt ttc atc acc agc gct gac tct gat       1872
Trp His Ile Asp Gly Val Lys Arg Phe Ile Thr Ser Ala Asp Ser Asp           
    610                 615                 620                           

gac ctg ttt gag aac atc ttc cac ttg gtt ttg gcg cgt ccg gaa ggc       1920
Asp Leu Phe Glu Asn Ile Phe His Leu Val Leu Ala Arg Pro Glu Gly           
625                 630                 635                 640           

gca ggc cct ggc acg aag ggt ctg agc ctg ttt ttc gtc ccg aaa ttc       1968
Ala Gly Pro Gly Thr Lys Gly Leu Ser Leu Phe Phe Val Pro Lys Phe           
                645                 650                 655               

tta ttc gat ttc gag act ggt gag ctg ggc gag cgt aat ggc gtg ttt       2016
Leu Phe Asp Phe Glu Thr Gly Glu Leu Gly Glu Arg Asn Gly Val Phe           
            660                 665                 670                   

gtc acc aac gtc gag cac aag atg ggt ctg aaa gtg agc gca acc tgt       2064
Val Thr Asn Val Glu His Lys Met Gly Leu Lys Val Ser Ala Thr Cys           
        675                 680                 685                       

gag ctg agc ttc ggt caa cac gat gtt ccg gcg aaa ggc tgg ctg gtt       2112
Glu Leu Ser Phe Gly Gln His Asp Val Pro Ala Lys Gly Trp Leu Val           
    690                 695                 700                           

ggt gag gtg cac aac ggt atc gcg cag atg ttt gag gtg att gaa cag       2160
Gly Glu Val His Asn Gly Ile Ala Gln Met Phe Glu Val Ile Glu Gln           
705                 710                 715                 720           

gcg cgt atg atg gtc ggt acc aag gca att gcc acc ctg agc acg ggt       2208
Ala Arg Met Met Val Gly Thr Lys Ala Ile Ala Thr Leu Ser Thr Gly           
                725                 730                 735               

tat ctg aat gca ctg gag tac gcg aaa agc cgc gtc caa ggt gcg gac       2256
Tyr Leu Asn Ala Leu Glu Tyr Ala Lys Ser Arg Val Gln Gly Ala Asp           
            740                 745                 750                   

atg acc cag atg acg gat aaa acg gct ccg cgt gtc acc att acc cac       2304
Met Thr Gln Met Thr Asp Lys Thr Ala Pro Arg Val Thr Ile Thr His           
        755                 760                 765                       

cat ccg gat gtc cgt cgc agc ctg atg acc caa aag gct tat gcc gag       2352
His Pro Asp Val Arg Arg Ser Leu Met Thr Gln Lys Ala Tyr Ala Glu           
    770                 775                 780                           

ggt ttg cgt gcg ctg tat ttg ttt acc tct acc tac caa gac gcg gca       2400
Gly Leu Arg Ala Leu Tyr Leu Phe Thr Ser Thr Tyr Gln Asp Ala Ala           
785                 790                 795                 800           

gtg gcc gag gcc ctg tac ggt gtg gac gcc gaa ttg gcg gtg aaa gtt       2448
Val Ala Glu Ala Leu Tyr Gly Val Asp Ala Glu Leu Ala Val Lys Val           
                805                 810                 815               

aat gac ctg atg ctg ccg gtc gtt aaa ggt gtg ggc agc gaa cag gcc       2496
Asn Asp Leu Met Leu Pro Val Val Lys Gly Val Gly Ser Glu Gln Ala           
            820                 825                 830                   

tac gcg aag ttg acg gag agc ctg cag acc ttt ggc ggt agc ggt ttt       2544
Tyr Ala Lys Leu Thr Glu Ser Leu Gln Thr Phe Gly Gly Ser Gly Phe           
        835                 840                 845                       

ctg cag gat tac ccg atc gag cag tat att cgc gat agc aaa atc gat       2592
Leu Gln Asp Tyr Pro Ile Glu Gln Tyr Ile Arg Asp Ser Lys Ile Asp           
    850                 855                 860                           

agc ctg tac gaa ggc acg acc gcg atc caa gcc cag gac ttt ttc ttt       2640
Ser Leu Tyr Glu Gly Thr Thr Ala Ile Gln Ala Gln Asp Phe Phe Phe           
865                 870                 875                 880           

cgc aag att gtt cgt gac aaa ggt gtg gcc ctg gcg cac gtg agc gag       2688
Arg Lys Ile Val Arg Asp Lys Gly Val Ala Leu Ala His Val Ser Glu           
                885                 890                 895               

caa att caa aag ttc gtc gac agc gag agc ggc aat ggt cgt ctg aag       2736
Gln Ile Gln Lys Phe Val Asp Ser Glu Ser Gly Asn Gly Arg Leu Lys           
            900                 905                 910                   

tcc gag aga gag ctg ctg gcg aaa gca ctg gcg gat gtt caa ggt atg       2784
Ser Glu Arg Glu Leu Leu Ala Lys Ala Leu Ala Asp Val Gln Gly Met           
        915                 920                 925                       

gcg act gcg ctg acg ggt tac ctg atg gct gca caa gaa gat gtg acc       2832
Ala Thr Ala Leu Thr Gly Tyr Leu Met Ala Ala Gln Glu Asp Val Thr           
    930                 935                 940                           

agc ctg tat aaa gtc ggc ctg ggc agc gtt cgt ttc ctg atg tcc gtg       2880
Ser Leu Tyr Lys Val Gly Leu Gly Ser Val Arg Phe Leu Met Ser Val           
945                 950                 955                 960           

ggc gat ctg atc atc ggc tgg ctg ctg cag cgt cag gca gct gtc gcg       2928
Gly Asp Leu Ile Ile Gly Trp Leu Leu Gln Arg Gln Ala Ala Val Ala           
                965                 970                 975               

gtc gcc gca ctg gac gcc ggt gcg tcc ggc gag gac cgc agc ttc tac       2976
Val Ala Ala Leu Asp Ala Gly Ala Ser Gly Glu Asp Arg Ser Phe Tyr           
            980                 985                 990                   

gaa ggc aag att gcc gtt gct agc  ttc ttt gcg aaa aac  ttt ctg ccg     3024
Glu Gly Lys Ile Ala Val Ala Ser  Phe Phe Ala Lys Asn  Phe Leu Pro         
        995                 1000                 1005                     

atg ctc  acg tcc acc cgt gaa  gtt atc gaa act ctg  gac aac gac        3069
Met Leu  Thr Ser Thr Arg Glu  Val Ile Glu Thr Leu  Asp Asn Asp            
    1010                 1015                 1020                        

att atg  gaa ctg gat gaa gcg  gcc ttt taa ctcgag                      3105
Ile Met  Glu Leu Asp Glu Ala  Ala Phe                                     
    1025                 1030                                             


<210>  18
<211>  1032
<212>  PRT
<213>  Mycobacterium avium

<400>  18

Met His His His His His His Ser Phe Val Thr Thr Gln Pro Glu Ala 
1               5                   10                  15      


Leu Ala Ala Ala Ala Gly Asn Leu Gln Ala Ile Gly Trp Thr Leu Ser 
            20                  25                  30          


Ala Gln Asn Ala Ala Ala Ala Ala Pro Thr Thr Gly Val Val Pro Ala 
        35                  40                  45              


Ala Ala Asp Glu Val Ser Ala Leu Thr Ala Ala Gln Phe Ala Ala Tyr 
    50                  55                  60                  


Ala Gln Met Tyr Gln Ala Val Ser Ala Gln Ala Ala Ala Ile His Glu 
65                  70                  75                  80  


Ala Phe Val Asn Thr Leu Ser Thr Ser Ser Val Ser Tyr Ala Ala Thr 
                85                  90                  95      


Glu Ala Ala Asn Ala Ala Ala Ala Leu Ser Ala Cys Tyr Cys Glu Leu 
            100                 105                 110         


Ser Met Ala Thr Arg Phe Met Thr Asp Pro His Glu Met Arg Ala Met 
        115                 120                 125             


Ala Gly Arg Phe Glu Val His Ala Gln Thr Val Glu Asp Glu Ala Arg 
    130                 135                 140                 


Lys Met Trp Ala Ser Ser Met Asn Ile Ala Gly Ala Gly Trp Ser Gly 
145                 150                 155                 160 


Gln Ala Gln Ala Thr Ser Tyr Asp Thr Met Gly Gln Val Asn Gln Ala 
                165                 170                 175     


Phe Arg Asn Ile Val Asn Met Leu His Gly Val Arg Asp Gly Leu Ile 
            180                 185                 190         


Arg Asp Ala Asn Asn Tyr Glu Gln Gln Glu Gln Ala Ser Gln Gln Ile 
        195                 200                 205             


Leu Ser Ser Gly Gly Ser Gly Gly Gly Ser Gly Met Ser Met Leu Asp 
    210                 215                 220                 


Ala His Ile Pro Gln Leu Val Ala Ala Gln Ser Ala Phe Ser Ala Lys 
225                 230                 235                 240 


Ala Ala Leu Met Arg Ser Thr Ile Ser Gln Ala Glu Gln Glu Ala Val 
                245                 250                 255     


Ser Ala Gln Ala Phe His Gln Gly Glu Ser Ser Ala Ala Phe Gln Ala 
            260                 265                 270         


Ala His Ala Arg Phe Val Glu Val Ala Ala Arg Val Asn Thr Leu Leu 
        275                 280                 285             


Asp Ile Ala Gln Ala Asn Leu Gly Asp Ala Ala Gly Thr Tyr Val Ala 
    290                 295                 300                 


Ala Asp Ala Ala Ala Ala Ser Gly Tyr Thr Ala Phe Gly Leu Val Pro 
305                 310                 315                 320 


Arg Gly Ser Thr Gly Met Ser Gln Ile Met Tyr Asn Tyr Pro Ala Met 
                325                 330                 335     


Leu Ser His Ala Ala Asp Met Ser Gly Tyr Ala Gly Thr Met Gln Gly 
            340                 345                 350         


Leu Gly Ala Asp Ile Ala Ser Glu Gln Ala Thr Leu Ser Asn Ala Trp 
        355                 360                 365             


Gln Gly Asp Thr Gly Met Thr Tyr Gln Val Trp Gln Ala Gln Trp Asn 
    370                 375                 380                 


Glu Ala Met Glu Ser Leu Val Arg Ala Tyr Gln Ser Met Ala Ser Thr 
385                 390                 395                 400 


His Glu Ala Asn Thr Met Ser Met Leu Ala Arg Asp Gln Ala Glu Ala 
                405                 410                 415     


Ala Lys Trp Gly Gly Met Ser His Tyr Lys Ser Asn Val Arg Asp Gln 
            420                 425                 430         


Glu Phe Asn Leu Phe Glu Val Leu Gly Val Asp Lys Ala Leu Gly Gln 
        435                 440                 445             


Gly Glu Phe Ser Asp Leu Asp Ala Asp Thr Ala Arg Glu Met Leu Asn 
    450                 455                 460                 


Glu Ile Ser Arg Leu Ala Glu Gly Pro Ile Ala Asp Ser Phe Val Glu 
465                 470                 475                 480 


Gly Asp Arg Asn Pro Pro Val Phe Asp Pro Lys Thr His Ser Val Thr 
                485                 490                 495     


Leu Pro Glu Ser Phe Lys Lys Ser Val His Ala Val Ile Glu Ala Gly 
            500                 505                 510         


Trp Asp Lys Val Gly Ile Asp Glu Ala Leu Gly Gly Val Ala Met Pro 
        515                 520                 525             


Lys Ala Leu Leu Trp Ala Leu His Glu His Ile Leu Gly Ala Asn Pro 
    530                 535                 540                 


Ala Val Trp Met Tyr Ala Gly Gly Ala Gly Phe Ala Asn Ile Leu Tyr 
545                 550                 555                 560 


His Leu Gly Thr Asp Glu Gln Lys Lys Trp Ala Val Met Ala Ala Glu 
                565                 570                 575     


Arg Gly Trp Gly Ser Thr Met Val Leu Thr Glu Pro Asp Ala Gly Ser 
            580                 585                 590         


Asp Val Gly Ala Gly Arg Thr Lys Ala Val Lys Gln Asp Asp Gly Ser 
        595                 600                 605             


Trp His Ile Asp Gly Val Lys Arg Phe Ile Thr Ser Ala Asp Ser Asp 
    610                 615                 620                 


Asp Leu Phe Glu Asn Ile Phe His Leu Val Leu Ala Arg Pro Glu Gly 
625                 630                 635                 640 


Ala Gly Pro Gly Thr Lys Gly Leu Ser Leu Phe Phe Val Pro Lys Phe 
                645                 650                 655     


Leu Phe Asp Phe Glu Thr Gly Glu Leu Gly Glu Arg Asn Gly Val Phe 
            660                 665                 670         


Val Thr Asn Val Glu His Lys Met Gly Leu Lys Val Ser Ala Thr Cys 
        675                 680                 685             


Glu Leu Ser Phe Gly Gln His Asp Val Pro Ala Lys Gly Trp Leu Val 
    690                 695                 700                 


Gly Glu Val His Asn Gly Ile Ala Gln Met Phe Glu Val Ile Glu Gln 
705                 710                 715                 720 


Ala Arg Met Met Val Gly Thr Lys Ala Ile Ala Thr Leu Ser Thr Gly 
                725                 730                 735     


Tyr Leu Asn Ala Leu Glu Tyr Ala Lys Ser Arg Val Gln Gly Ala Asp 
            740                 745                 750         


Met Thr Gln Met Thr Asp Lys Thr Ala Pro Arg Val Thr Ile Thr His 
        755                 760                 765             


His Pro Asp Val Arg Arg Ser Leu Met Thr Gln Lys Ala Tyr Ala Glu 
    770                 775                 780                 


Gly Leu Arg Ala Leu Tyr Leu Phe Thr Ser Thr Tyr Gln Asp Ala Ala 
785                 790                 795                 800 


Val Ala Glu Ala Leu Tyr Gly Val Asp Ala Glu Leu Ala Val Lys Val 
                805                 810                 815     


Asn Asp Leu Met Leu Pro Val Val Lys Gly Val Gly Ser Glu Gln Ala 
            820                 825                 830         


Tyr Ala Lys Leu Thr Glu Ser Leu Gln Thr Phe Gly Gly Ser Gly Phe 
        835                 840                 845             


Leu Gln Asp Tyr Pro Ile Glu Gln Tyr Ile Arg Asp Ser Lys Ile Asp 
    850                 855                 860                 


Ser Leu Tyr Glu Gly Thr Thr Ala Ile Gln Ala Gln Asp Phe Phe Phe 
865                 870                 875                 880 


Arg Lys Ile Val Arg Asp Lys Gly Val Ala Leu Ala His Val Ser Glu 
                885                 890                 895     


Gln Ile Gln Lys Phe Val Asp Ser Glu Ser Gly Asn Gly Arg Leu Lys 
            900                 905                 910         


Ser Glu Arg Glu Leu Leu Ala Lys Ala Leu Ala Asp Val Gln Gly Met 
        915                 920                 925             


Ala Thr Ala Leu Thr Gly Tyr Leu Met Ala Ala Gln Glu Asp Val Thr 
    930                 935                 940                 


Ser Leu Tyr Lys Val Gly Leu Gly Ser Val Arg Phe Leu Met Ser Val 
945                 950                 955                 960 


Gly Asp Leu Ile Ile Gly Trp Leu Leu Gln Arg Gln Ala Ala Val Ala 
                965                 970                 975     


Val Ala Ala Leu Asp Ala Gly Ala Ser Gly Glu Asp Arg Ser Phe Tyr 
            980                 985                 990         


Glu Gly Lys Ile Ala Val Ala Ser  Phe Phe Ala Lys Asn  Phe Leu Pro 
        995                 1000                 1005             


Met Leu  Thr Ser Thr Arg Glu  Val Ile Glu Thr Leu  Asp Asn Asp 
    1010                 1015                 1020             


Ile Met  Glu Leu Asp Glu Ala  Ala Phe 
    1025                 1030         


