                         SEQUENCE LISTING

<110>  Merck Sharp & Dohme Corp.
       Choi, Byung-Kwon
 
<120>  METHODS FOR INCREASING N-GLYCAN OCCUPANCY AND REDUCING PRODUCTION
        OF HYBRID N-GLYCANS IN PICHIA PASTORIS STRAINS LACKING ALG3 
       EXPRESSION

<130>  23434

<150>  61/764,198
<151>  2013-02-13

<160>  62    

<170>  PatentIn version 3.5

<210>  1
<211>  857
<212>  PRT
<213>  Leishmania major

<400>  1

Met Gly Lys Arg Lys Gly Asn Ser Leu Gly Asp Ser Gly Ser Ala Ala 
1               5                   10                  15      


Thr Ala Ser Arg Glu Ala Ser Ala Gln Ala Glu Asp Ala Ala Ser Gln 
            20                  25                  30          


Thr Lys Thr Ala Ser Pro Pro Ala Lys Val Ile Leu Leu Pro Lys Thr 
        35                  40                  45              


Leu Thr Asp Glu Lys Asp Phe Ile Gly Ile Phe Pro Phe Pro Phe Trp 
    50                  55                  60                  


Pro Val His Phe Val Leu Thr Val Val Ala Leu Phe Val Leu Ala Ala 
65                  70                  75                  80  


Ser Cys Phe Gln Ala Phe Thr Val Arg Met Ile Ser Val Gln Ile Tyr 
                85                  90                  95      


Gly Tyr Leu Ile His Glu Phe Asp Pro Trp Phe Asn Tyr Arg Ala Ala 
            100                 105                 110         


Glu Tyr Met Ser Thr His Gly Trp Ser Ala Phe Phe Ser Trp Phe Asp 
        115                 120                 125             


Tyr Met Ser Trp Tyr Pro Leu Gly Arg Pro Val Gly Ser Thr Thr Tyr 
    130                 135                 140                 


Pro Gly Leu Gln Leu Thr Ala Val Ala Ile His Arg Ala Leu Ala Ala 
145                 150                 155                 160 


Ala Gly Met Pro Met Ser Leu Asn Asn Val Cys Val Leu Met Pro Ala 
                165                 170                 175     


Trp Phe Gly Ala Ile Ala Thr Ala Thr Leu Ala Phe Cys Thr Tyr Glu 
            180                 185                 190         


Ala Ser Gly Ser Thr Val Ala Ala Ala Ala Ala Ala Leu Ser Phe Ser 
        195                 200                 205             


Ile Ile Pro Ala His Leu Met Arg Ser Met Ala Gly Glu Phe Asp Asn 
    210                 215                 220                 


Glu Cys Ile Ala Val Ala Ala Met Leu Leu Thr Phe Tyr Cys Trp Val 
225                 230                 235                 240 


Arg Ser Leu Arg Thr Arg Ser Ser Trp Pro Ile Gly Val Leu Thr Gly 
                245                 250                 255     


Val Ala Tyr Gly Tyr Met Ala Ala Ala Trp Gly Gly Tyr Ile Phe Val 
            260                 265                 270         


Leu Asn Met Val Ala Met His Ala Gly Ile Ser Ser Met Val Asp Trp 
        275                 280                 285             


Ala Arg Asn Thr Tyr Asn Pro Ser Leu Leu Arg Ala Tyr Thr Leu Phe 
    290                 295                 300                 


Tyr Val Val Gly Thr Ala Ile Ala Val Cys Val Pro Pro Val Gly Met 
305                 310                 315                 320 


Ser Pro Phe Lys Ser Leu Glu Gln Leu Gly Ala Leu Leu Val Leu Val 
                325                 330                 335     


Phe Leu Cys Gly Leu Gln Val Cys Glu Val Leu Arg Ala Arg Ala Gly 
            340                 345                 350         


Val Glu Val Arg Ser Arg Ala Asn Phe Lys Ile Arg Val Arg Val Phe 
        355                 360                 365             


Ser Val Met Ala Gly Val Ala Ala Leu Ala Ile Ser Val Leu Ala Pro 
    370                 375                 380                 


Thr Gly Tyr Phe Gly Pro Leu Ser Val Arg Val Arg Ala Leu Phe Val 
385                 390                 395                 400 


Glu His Thr Arg Thr Gly Asn Pro Leu Val Asp Ser Val Ala Glu His 
                405                 410                 415     


Gln Pro Ala Ser Pro Glu Ala Met Trp Ala Phe Leu His Val Cys Gly 
            420                 425                 430         


Val Thr Trp Gly Leu Gly Ser Ile Val Leu Ala Val Ser Thr Phe Val 
        435                 440                 445             


His Tyr Ser Pro Ser Lys Val Phe Trp Leu Leu Asn Ser Gly Ala Val 
    450                 455                 460                 


Tyr Tyr Phe Ser Thr Arg Met Ala Arg Leu Leu Leu Leu Ser Gly Pro 
465                 470                 475                 480 


Ala Ala Cys Leu Ser Thr Gly Ile Phe Val Gly Thr Ile Leu Glu Ala 
                485                 490                 495     


Ala Val Gln Leu Ser Phe Trp Asp Ser Asp Ala Thr Lys Ala Lys Lys 
            500                 505                 510         


Gln Gln Lys Gln Ala Gln Arg His Gln Arg Gly Ala Gly Lys Gly Ser 
        515                 520                 525             


Gly Arg Asp Asp Ala Lys Asn Ala Thr Thr Ala Arg Ala Phe Cys Asp 
    530                 535                 540                 


Val Phe Ala Gly Ser Ser Leu Ala Trp Gly His Arg Met Val Leu Ser 
545                 550                 555                 560 


Ile Ala Met Trp Ala Leu Val Thr Thr Thr Ala Val Ser Phe Phe Ser 
                565                 570                 575     


Ser Glu Phe Ala Ser His Ser Thr Lys Phe Ala Glu Gln Ser Ser Asn 
            580                 585                 590         


Pro Met Ile Val Phe Ala Ala Val Val Gln Asn Arg Ala Thr Gly Lys 
        595                 600                 605             


Pro Met Asn Leu Leu Val Asp Asp Tyr Leu Lys Ala Tyr Glu Trp Leu 
    610                 615                 620                 


Arg Asp Ser Thr Pro Glu Asp Ala Arg Val Leu Ala Trp Trp Asp Tyr 
625                 630                 635                 640 


Gly Tyr Gln Ile Thr Gly Ile Gly Asn Arg Thr Ser Leu Ala Asp Gly 
                645                 650                 655     


Asn Thr Trp Asn His Glu His Ile Ala Thr Ile Gly Lys Met Leu Thr 
            660                 665                 670         


Ser Pro Val Val Glu Ala His Ser Leu Val Arg His Met Ala Asp Tyr 
        675                 680                 685             


Val Leu Ile Trp Ala Gly Gln Ser Gly Asp Leu Met Lys Ser Pro His 
    690                 695                 700                 


Met Ala Arg Ile Gly Asn Ser Val Tyr His Asp Ile Cys Pro Asp Asp 
705                 710                 715                 720 


Pro Leu Cys Gln Gln Phe Gly Phe His Arg Asn Asp Tyr Ser Arg Pro 
                725                 730                 735     


Thr Pro Met Met Arg Ala Ser Leu Leu Tyr Asn Leu His Glu Ala Gly 
            740                 745                 750         


Lys Arg Lys Gly Val Lys Val Asn Pro Ser Leu Phe Gln Glu Val Tyr 
        755                 760                 765             


Ser Ser Lys Tyr Gly Leu Val Arg Ile Phe Lys Val Met Asn Val Ser 
    770                 775                 780                 


Ala Glu Ser Lys Lys Trp Val Ala Asp Pro Ala Asn Arg Val Cys His 
785                 790                 795                 800 


Pro Pro Gly Ser Trp Ile Cys Pro Gly Gln Tyr Pro Pro Ala Lys Glu 
                805                 810                 815     


Ile Gln Glu Met Leu Ala His Arg Val Pro Phe Asp Gln Val Thr Asn 
            820                 825                 830         


Ala Asp Arg Lys Asn Asn Val Gly Ser Tyr Gln Glu Glu Tyr Met Arg 
        835                 840                 845             


Arg Met Arg Glu Ser Glu Asn Arg Arg 
    850                 855         


<210>  2
<211>  2577
<212>  DNA
<213>  Leishmania major

<400>  2
atgggtaaaa gaaagggaaa ctccttggga gattctggtt ctgctgctac tgcttccaga       60

gaggcttctg ctcaagctga agatgctgct tcccagacta agactgcttc tccacctgct      120

aaggttatct tgttgccaaa gactttgact gacgagaagg acttcatcgg tatcttccca      180

tttccattct ggccagttca cttcgttttg actgttgttg ctttgttcgt tttggctgct      240

tcctgtttcc aggctttcac tgttagaatg atctccgttc aaatctacgg ttacttgatc      300

cacgaatttg acccatggtt caactacaga gctgctgagt acatgtctac tcacggatgg      360

agtgcttttt tctcctggtt cgattacatg tcctggtatc cattgggtag accagttggt      420

tctactactt acccaggatt gcagttgact gctgttgcta tccatagagc tttggctgct      480

gctggaatgc caatgtcctt gaacaatgtt tgtgttttga tgccagcttg gtttggtgct      540

atcgctactg ctactttggc tttctgtact tacgaggctt ctggttctac tgttgctgct      600

gctgcagctg ctttgtcctt ctccattatc cctgctcact tgatgagatc catggctggt      660

gagttcgaca acgagtgtat tgctgttgct gctatgttgt tgactttcta ctgttgggtt      720

cgttccttga gaactagatc ctcctggcca atcggtgttt tgacaggtgt tgcttacggt      780

tacatggctg ctgcttgggg aggttacatc ttcgttttga acatggttgc tatgcacgct      840

ggtatctctt ctatggttga ctgggctaga aacacttaca acccatcctt gttgagagct      900

tacactttgt tctacgttgt tggtactgct atcgctgttt gtgttccacc agttggaatg      960

tctccattca agtccttgga gcagttggga gctttgttgg ttttggtttt cttgtgtgga     1020

ttgcaagttt gtgaggtttt gagagctaga gctggtgttg aagttagatc cagagctaat     1080

ttcaagatca gagttagagt tttctccgtt atggctggtg ttgctgcttt ggctatctct     1140

gttttggctc caactggtta ctttggtcca ttgtctgtta gagttagagc tttgtttgtt     1200

gagcacacta gaactggtaa cccattggtt gactccgttg ctgaacatca accagcttct     1260

ccagaggcta tgtgggcttt cttgcatgtt tgtggtgtta cttggggatt gggttccatt     1320

gttttggctg tttccacttt cgttcactac tccccatcta aggttttctg gttgttgaac     1380

tccggtgctg tttactactt ctccactaga atggctagat tgttgttgtt gtccggtcca     1440

gctgcttgtt tgtccactgg tatcttcgtt ggtactatct tggaggctgc tgttcaattg     1500

tctttctggg actccgatgc tactaaggct aagaagcagc aaaagcaggc tcaaagacac     1560

caaagaggtg ctggtaaagg ttctggtaga gatgacgcta agaacgctac tactgctaga     1620

gctttctgtg acgttttcgc tggttcttct ttggcttggg gtcacagaat ggttttgtcc     1680

attgctatgt gggctttggt tactactact gctgtttcct tcttctcctc cgaatttgct     1740

tctcactcca ctaagttcgc tgaacaatcc tccaacccaa tgatcgtttt cgctgctgtt     1800

gttcagaaca gagctactgg aaagccaatg aacttgttgg ttgacgacta cttgaaggct     1860

tacgagtggt tgagagactc tactccagag gacgctagag ttttggcttg gtgggactac     1920

ggttaccaaa tcactggtat cggtaacaga acttccttgg ctgatggtaa cacttggaac     1980

cacgagcaca ttgctactat cggaaagatg ttgacttccc cagttgttga agctcactcc     2040

cttgttagac acatggctga ctacgttttg atttgggctg gtcaatctgg tgacttgatg     2100

aagtctccac acatggctag aatcggtaac tctgtttacc acgacatttg tccagatgac     2160

ccattgtgtc agcaattcgg tttccacaga aacgattact ccagaccaac tccaatgatg     2220

agagcttcct tgttgtacaa cttgcacgag gctggaaaaa gaaagggtgt taaggttaac     2280

ccatctttgt tccaagaggt ttactcctcc aagtacggac ttgttagaat cttcaaggtt     2340

atgaacgttt ccgctgagtc taagaagtgg gttgcagacc cagctaacag agtttgtcac     2400

ccacctggtt cttggatttg tcctggtcaa tacccacctg ctaaagaaat ccaagagatg     2460

ttggctcaca gagttccatt cgaccaggtt acaaacgctg acagaaagaa caatgttggt     2520

tcctaccaag aggaatacat gagaagaatg agagagtccg agaacagaag ataatag        2577


<210>  3
<211>  934
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AOX1 promoter

<400>  3
aacatccaaa gacgaaaggt tgaatgaaac ctttttgcca tccgacatcc acaggtccat       60

tctcacacat aagtgccaaa cgcaacagga ggggatacac tagcagcaga ccgttgcaaa      120

cgcaggacct ccactcctct tctcctcaac acccactttt gccatcgaaa aaccagccca      180

gttattgggc ttgattggag ctcgctcatt ccaattcctt ctattaggct actaacacca      240

tgactttatt agcctgtcta tcctggcccc cctggcgagg ttcatgtttg tttatttccg      300

aatgcaacaa gctccgcatt acacccgaac atcactccag atgagggctt tctgagtgtg      360

gggtcaaata gtttcatgtt ccccaaatgg cccaaaactg acagtttaaa cgctgtcttg      420

gaacctaata tgacaaaagc gtgatctcat ccaagatgaa ctaagtttgg ttcgttgaaa      480

tgctaacggc cagttggtca aaaagaaact tccaaaagtc ggcataccgt ttgtcttgtt      540

tggtattgat tgacgaatgc tcaaaaataa tctcattaat gcttagcgca gtctctctat      600

cgcttctgaa ccccggtgca cctgtgccga aacgcaaatg gggaaacacc cgctttttgg      660

atgattatgc attgtctcca cattgtatgc ttccaagatt ctggtgggaa tactgctgat      720

agcctaacgt tcatgatcaa aatttaactg ttctaacccc tacttgacag caatatataa      780

acagaaggaa gctgccctgt cttaaacctt tttttttatc atcattatta gcttactttc      840

ataattgcga ctggttccaa ttgacaagct tttgatttta acgactttta acgacaactt      900

gagaagatca aaaaacaact aattattcga aacg                                  934


<210>  4
<211>  293
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ScCYC TT

<400>  4
acaggcccct tttcctttgt cgatatcatg taattagtta tgtcacgctt acattcacgc       60

cctcctccca catccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc      120

cctatttatt ttttttaata gttatgttag tattaagaac gttatttata tttcaaattt      180

ttcttttttt tctgtacaaa cgcgtgtacg catgtaacat tatactgaaa accttgcttg      240

agaaggtttt gggacgctcg aaggctttaa tttgcaagct gccggctctt aag             293


<210>  5
<211>  1215
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ScARR3 ORF

<400>  5
atgtcagaag atcaaaaaag tgaaaattcc gtaccttcta aggttaatat ggtgaatcgc       60

accgatatac tgactacgat caagtcattg tcatggcttg acttgatgtt gccatttact      120

ataattctct ccataatcat tgcagtaata atttctgtct atgtgccttc ttcccgtcac      180

acttttgacg ctgaaggtca tcccaatcta atgggagtgt ccattccttt gactgttggt      240

atgattgtaa tgatgattcc cccgatctgc aaagtttcct gggagtctat tcacaagtac      300

ttctacagga gctatataag gaagcaacta gccctctcgt tatttttgaa ttgggtcatc      360

ggtcctttgt tgatgacagc attggcgtgg atggcgctat tcgattataa ggaataccgt      420

caaggcatta ttatgatcgg agtagctaga tgcattgcca tggtgctaat ttggaatcag      480

attgctggag gagacaatga tctctgcgtc gtgcttgtta ttacaaactc gcttttacag      540

atggtattat atgcaccatt gcagatattt tactgttatg ttatttctca tgaccacctg      600

aatacttcaa atagggtatt attcgaagag gttgcaaagt ctgtcggagt ttttctcggc      660

ataccactgg gaattggcat tatcatacgt ttgggaagtc ttaccatagc tggtaaaagt      720

aattatgaaa aatacatttt gagatttatt tctccatggg caatgatcgg atttcattac      780

actttatttg ttatttttat tagtagaggt tatcaattta tccacgaaat tggttctgca      840

atattgtgct ttgtcccatt ggtgctttac ttctttattg catggttttt gaccttcgca      900

ttaatgaggt acttatcaat atctaggagt gatacacaaa gagaatgtag ctgtgaccaa      960

gaactacttt taaagagggt ctggggaaga aagtcttgtg aagctagctt ttctattacg     1020

atgacgcaat gtttcactat ggcttcaaat aattttgaac tatccctggc aattgctatt     1080

tccttatatg gtaacaatag caagcaagca atagctgcaa catttgggcc gttgctagaa     1140

gttccaattt tattgatttt ggcaatagtc gcgagaatcc ttaaaccata ttatatatgg     1200

aacaatagaa attaa                                                      1215


<210>  6
<211>  600
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PpRPL10 promoter

<400>  6
gttcttcgct tggtcttgta tctccttaca ctgtatcttc ccatttgcgt ttaggtggtt       60

atcaaaaact aaaaggaaaa atttcagatg tttatctcta aggttttttc tttttacagt      120

ataacacgtg atgcgtcacg tggtactaga ttacgtaagt tattttggtc cggtgggtaa      180

gtgggtaaga atagaaagca tgaaggttta caaaaacgca gtcacgaatt attgctactt      240

cgagcttgga accaccccaa agattatatt gtactgatgc actaccttct cgattttgct      300

cctccaagaa cctacgaaaa acatttcttg agccttttca acctagacta cacatcaagt      360

tatttaaggt atgttccgtt aacatgtaag aaaaggagag gatagatcgt ttatggggta      420

cgtcgcctga ttcaagcgtg accattcgaa gaataggcct tcgaaagctg aataaagcaa      480

atgtcagttg cgattggtat gctgacaaat tagcataaaa agcaatagac tttctaacca      540

cctgtttttt tccttttact ttatttatat tttgccaccg tactaacaag ttcagacaaa      600


<210>  7
<211>  1144
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  URA6 region

<400>  7
caaatgcaag aggacattag aaatgtgttt ggtaagaaca tgaagccgga ggcatacaaa       60

cgattcacag atttgaagga ggaaaacaaa ctgcatccac cggaagtgcc agcagccgtg      120

tatgccaacc ttgctctcaa aggcattcct acggatctga gtgggaaata tctgagattc      180

acagacccac tattggaaca gtaccaaacc tagtttggcc gatccatgat tatgtaatgc      240

atatagtttt tgtcgatgct cacccgtttc gagtctgtct cgtatcgtct tacgtataag      300

ttcaagcatg tttaccaggt ctgttagaaa ctcctttgtg agggcaggac ctattcgtct      360

cggtcccgtt gtttctaaga gactgtacag ccaagcgcag aatggtggca ttaaccataa      420

gaggattctg atcggacttg gtctattggc tattggaacc accctttacg ggacaaccaa      480

ccctaccaag actcctattg catttgtgga accagccacg gaaagagcgt ttaaggacgg      540

agacgtctct gtgatttttg ttctcggagg tccaggagct ggaaaaggta cccaatgtgc      600

caaactagtg agtaattacg gatttgttca cctgtcagct ggagacttgt tacgtgcaga      660

acagaagagg gaggggtcta agtatggaga gatgatttcc cagtatatca gagatggact      720

gatagtacct caagaggtca ccattgcgct cttggagcag gccatgaagg aaaacttcga      780

gaaagggaag acacggttct tgattgatgg attccctcgt aagatggacc aggccaaaac      840

ttttgaggaa aaagtcgcaa agtccaaggt gacacttttc tttgattgtc ccgaatcagt      900

gctccttgag agattactta aaagaggaca gacaagcgga agagaggatg ataatgcgga      960

gagtatcaaa aaaagattca aaacattcgt ggaaacttcg atgcctgtgg tggactattt     1020

cgggaagcaa ggacgcgttt tgaaggtatc ttgtgaccac cctgtggatc aagtgtattc     1080

acaggttgtg tcggtgctaa aagagaaggg gatctttgcc gataacgaga cggagaataa     1140

ataa                                                                  1144


<210>  8
<211>  486
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PpGAPDH promoter

<400>  8
tttttgtaga aatgtcttgg tgtcctcgtc caatcaggta gccatctctg aaatatctgg       60

ctccgttgca actccgaacg acctgctggc aacgtaaaat tctccggggt aaaacttaaa      120

tgtggagtaa tggaaccaga aacgtctctt cccttctctc tccttccacc gcccgttacc      180

gtccctagga aattttactc tgctggagag cttcttctac ggcccccttg cagcaatgct      240

cttcccagca ttacgttgcg ggtaaaacgg aggtcgtgta cccgacctag cagcccaggg      300

atggaaaagt cccggccgtc gctggcaata atagcgggcg gacgcatgtc atgagattat      360

tggaaaccac cagaatcgaa tataaaaggc gaacaccttt cccaattttg gtttctcctg      420

acccaaagac tttaaattta atttatttgt ccctatttca atcaattgaa caactatcaa      480

aacaca                                                                 486


<210>  9
<211>  573
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NatR ORF

<400>  9
atgggtacca ctcttgacga cacggcttac cggtaccgca ccagtgtccc gggggacgcc       60

gaggccatcg aggcactgga tgggtccttc accaccgaca ccgtcttccg cgtcaccgcc      120

accggggacg gcttcaccct gcgggaggtg ccggtggacc cgcccctgac caaggtgttc      180

cccgacgacg aatcggacga cgaatcggac gacggggagg acggcgaccc ggactcccgg      240

acgttcgtcg cgtacgggga cgacggcgac ctggcgggct tcgtggtcat ctcgtactcg      300

gcgtggaacc gccggctgac cgtcgaggac atcgaggtcg ccccggagca ccgggggcac      360

ggggtcgggc gcgcgttgat ggggctcgcg acggagttcg ccggcgagcg gggcgccggg      420

cacctctggc tggaggtcac caacgtcaac gcaccggcga tccacgcgta ccggcggatg      480

gggttcaccc tctgcggcct ggacaccgcc ctgtacgacg gcaccgcctc ggacggcgag      540

cggcaggcgc tctacatgag catgccctgc ccc                                   573


<210>  10
<211>  388
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ashbya gossypii TEF1 promoter

<400>  10
gatctgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg gaggcccaga       60

ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt      120

acatttagcc catacatccc catgtataat catttgcatc catacatttt gatggccgca      180

cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga aacgctcccc      240

tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata aaaggttagg      300

atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta ggatacagtt      360

ctcacatcac atccgaacat aaacaacc                                         388


<210>  11
<211>  247
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ashbya gossypii TEF1 termination sequence

<400>  11
taatcagtac tgacaataaa aagattcttg ttttcaagaa cttgtcattt gtatagtttt       60

tttatattgt agttgttcta ttttaatcaa atgttagcgt gatttatatt ttttttcgcc      120

tcgacatcat ctgcccagat gcgaagttaa gtgcgcagaa agtaatatca tgcgtcaatc      180

gtatgtgaat gctggtcgct atactgctgt cgattcgata ctaacgccgc catccagtgt      240

cgaaaac                                                                247


<210>  12
<211>  2409
<212>  DNA
<213>  Trypanosoma brucei

<400>  12
atgaccaagg gaggaaaagt tgctgttaca aagggtagtg cccaatctga cggtgctgga       60

gagggaggaa tgagtaaagc caagagttca actacatttg ttgctaccgg tggaggtagt      120

cttccagctt gggccttgaa agctgtttct actattgtct ccgccgttat tttgatctac      180

tctgtccata gagcttatga tattagattg acctccgtta gattgtacgg tgaacttatc      240

cacgagttcg acccttggtt taactacaga gccactcaat atttgtctga taatggatgg      300

agagcatttt tccagtggta cgactatatg tcctggtatc cattgggaag acctgttggt      360

accactattt ttcctggtat gcaattgact ggagtcgcta tccatagagt tcttgaaatg      420

ttgggaagag gaatgtcaat taacaacatc tgtgtctaca ttcctgcctg gtttggtagt      480

atcgcaacag ttcttgctgc cttgattgct tacgagtctt ccaactcatt gagtgtcatg      540

gcattcaccg cttacttttt ctctatcgtt ccagcacact tgatgagatc catggctggt      600

gaatttgata atgagtgtgt tgctatggca gctatgttgc ttactttcta catgtgggtt      660

agatccctta gatcaagttc ttcctggcct attggagcat tggctggtgt cgcttacgga      720

tatatggttt ctacatgggg aggttatatc ttcgtcttga acatggttgc ctttcatgca      780

tccgtctgtg ttttgcttga ttgggctaga ggtatctact ctgtctcctt gcttagagcc      840

tattctttgt ttttcgttat tggaactgcc ttggcaatct gcgtcccacc tgttgaatgg      900

acacctttta gatcccttga gcaattgacc gccctttttg tcttcgtttt tatgtgggca      960

cttcattact ctgaatattt gagagagaga gctagagccc ctattcactc aagtaaagcc     1020

ttgcagatta gagcaagaat cttcatgggt actttgtcat tgcttttgat tgttgcaagt     1080

cttttggctc catttggatt tttcaaacct acagcttaca gagtcagagc cttgttcgtt     1140

aagcacacca gaactggtaa cccattggtc gattcagttg ctgaacatag acctacaacc     1200

gcaggtgctt accttagata ttttcacgtt tgttacccat tgtggggatg cggaggtttg     1260

tccatgcttg ttttcatgaa gaaagacaga tggagagcaa ttgtcttttt ggcttcactt     1320

agtacagtta ccatgtattt ctcagctaga atgagtagac ttttgctttt ggccggtcct     1380

gccgcaactg cctgtgcagg aatgttcatt ggaggtttgt ttgatcttgc tttgtctcaa     1440

ttcggagatt tgcattcccc aaaggacgcc tctggagatt ccgaccctgc tggaggttcc     1500

aaaagagcca agggtaaagt tgtcaatgaa ccatctaaga gagctatttt ctcccacaga     1560

tggtttcaaa gattggtcca gtcacttcca gttcctttga gaagaggtat cgcagttgtc     1620

gttttggttt gtcttttcgc taaccctatg agacattctt ttgaaaagtc ctgcgagaaa     1680

atggctcacg ccttgtcttc cccaagaatt atcgccgtta ctgatttgcc taatggtgaa     1740

agagtcttgg cagatgacta ctatgtttca tacctttggt tgagaaacaa taccccagag     1800

gatgctagaa ttttgagttg gtgggactac ggttatcaaa ttactggaat cggtaacaga     1860

actacattgg cagatggtaa tacatggtct cataagcaca ttgctaccat cggaaaaatg     1920

ttgacttcac ctgttaaaga aagtcatgca cttattagac acttggctga ctacgttttg     1980

atctgggctg gagaggatag aggagacctt ttgaaatctc cacatatggc tagaatcggt     2040

aactcagttt acagagatat gtgtagtgaa gatgacccta gatgcagaca attcggattt     2100

gagggtggtg acttgaacaa gccaactcct atgatgcaga gatccctttt gtacaatttg     2160

cacagatttg gtactgatgg aggtaaaaca caacttgaca agaacatgtt ccagttggct     2220

tacgtctcca agtatggatt ggttaaaatc tacaaagtcg ttaacgtttc agaagagagt     2280

aaagcttggg tcgccgatcc aaagaataga gtttgtgacc cacctggttc ttggatttgc     2340

gccggacaat acccacctgc aaaagaaatc caggatatgt tggctaagag atttcattat     2400

gagtaatga                                                             2409


<210>  13
<211>  2469
<212>  DNA
<213>  Trypanosoma brucei

<400>  13
atgaccaagg gaggaaaggt tgctgttact aaaggttctg ctcaatccga cggtgctgga       60

gagggaggaa tgtcaaaagc caagagtagt actacatttg ttgctaccgg tggaggttct      120

cttccagcat gggctttgaa ggccgtttca actgttgtca gtgcagttat tttgatctac      180

tccgtccata gagcttacga tatcagattg acatcagtta gactttatgg tgaattgatc      240

cacgagtttg acccttggtt caactacaga gcaacccaat atttgtccga taatggatgg      300

agagctttct ttcagtggta cgactatatg tcatggtacc cattgggaag acctgttggt      360

accactattt ttcctggtat gcaattgact ggagttgcta tccatagagt cttggaaatg      420

cttggaagag gaatgtcaat taacaacatc tgtgtttaca tccctgcttg gtttggttct      480

atcgccactg tcttggctgc ccttattgct tacgagtctt ccaactcatt gagtgttatg      540

gccttcacag catacttttt ctctattgtc ccagctcact tgatgagatc aatggccggt      600

gaatttgata atgagtgtgt tgctatggca gctatgttgc ttactttcta tatgtgggtt      660

agatccctta gatcaagttc ttcctggcct attggagcct tggcaggtgt tgcttacgga      720

tatatggtct caacttgggg aggttacatc tttgttttga acatggtcgc tttccatgcc      780

tctgtttgtg tcttgcttga ttgggccaga ggtacatact ctgtttcctt gcttagagca      840

tattctttgt ttttcgtcat tggaaccgct ttggccatct gcgttccacc tgtcgaatgg      900

actcctttta gatccttgga gcaacttaca gccttgttcg tttttgtctt catgtgggca      960

cttcattact ctgaatattt gagagagaga gcaagagctc ctattcactc tagtaaggca     1020

ttgcagatta gagctagaat ctttatgggt actcttagtt tgcttttgat tgttgctatc     1080

tacttgttct ccacaggata ttttagacca ttctcttcca gagttagagc tttgttcgtc     1140

aaacacacta gaacaggtaa tccattggtt gatagtgtcg ccgaacatca ccctgcatct     1200

aacgatgact ttttcggata cttgcatgtt tgttacaacg gttggatcat cggatttttc     1260

tttatgtcag ttagttgttt ctttcactgc actccaggaa tgtcatttct tttgctttac     1320

agtatccttg cttactactt ctctttgaag atgtccagat tgcttttgct ttctgcacct     1380

gttgcttcca ttttgaccgg ttacgttgtc ggatctatcg ttgatttggc cgcagactgt     1440

tttgctgcca gtggtactga acatgctgat tctaaggagc accaaggaaa agccagagga     1500

aagggtcaaa aagaacagat tactgttgag tgtggttgcc ataacccttt ttacaagctt     1560

tggtgtaatt ccttctcaag tagattggtt gtcggaaaat tctttgttgt cgttgtcctt     1620

tcaatttgcg gtccaacttt cttgggttct aacttcagaa tctattccga acaattcgca     1680

gattcaatgt cttcccctca gattatcatg agagccactg ttggaggtag aagagtcatt     1740

ttggatgact actatgtttc ttacttgtgg cttagaaaca atacaccaga ggatgctaga     1800

attttgtcct ggtgggacta cggttatcaa attaccggaa tcggtaacag aacaaccttg     1860

gctgatggta acacttggaa tcatgaacac attgccacaa tcggaaagat gttgacctca     1920

cctgttaaag agagtcatgc acttattaga cacttggctg actacgtttt gatctgggct     1980

ggatatgatg gttctgactt gcttaagtcc ccacatatgg ctagaattgg taattccgtt     2040

tacagagata tctgttcaga agatgaccct ttgtgcacac aatttggttt ctattcagga     2100

gactttagta aaccaacccc tatgatgcag agatccttgc tttacaactt gcacagattt     2160

ggtaccgatg gaggtaaaac tcaacttgac aagaacatgt tccagttggc ttacgtttct     2220

aagtatggat tggtcaaaat ctacaaagtt atgaacgtct ctgaagagtc caaagcctgg     2280

gttgcagatc caaagaatag aaaatgtgac gctcctggtt cttggatttg cactggacaa     2340

tacccacctg caaaggaaat tcaggatatg cttgctaaaa gaatcgatta tgaacaattg     2400

gaggacttta acagaagaaa tagatccgac gcttactata gagcctacat gagacagatg     2460

ggttaatga                                                             2469


<210>  14
<211>  2469
<212>  DNA
<213>  Trypanosoma brucei

<400>  14
atgactaagg gtggtaaagt tgctgttact aagggttctg ctcaatctga tggtgctggt       60

gaaggtggaa tgtctaaggc taagtcctcc actactttcg ttgctactgg tggtggttct      120

ttgccagctt gggctttgaa ggctgtttcc actgttgttt ccgctgttat cttgatctac      180

tccgttcaca gagcttacga catcagattg acttcagtta gattgtacgg tgagttgatc      240

cacgaatttg acccatggtt caactacaga gctactcagt acttgtctga caacggatgg      300

agagctttct tccagtggta cgattacatg tcctggtatc cattgggtag accagttggt      360

actactatct tcccaggaat gcagttgact ggagttgcta tccacagagt tttggagatg      420

ttgggtagag gaatgtccat caacaacatc tgtgtttaca tcccagcttg gtttggttcc      480

attgctactg ttttggctgc tttgatcgct tacgaatcct ctaactcctt gtccgttatg      540

gctttcactg cttacttttt ctccatcgtt cctgctcatt tgatgagatc catggctggt      600

gagttcgaca acgagtgtgt tgctatggct gctatgttgt tgactttcta catgtgggtt      660

cgttccttga gatcctcttc ctcttggcca attggtgctt tggctggtgt tgcttacggt      720

tacatggttt ccacttgggg aggttacatc ttcgttttga acatggttgc tttccacgct      780

tccgtttgtg ttttgttgga ctgggctaga ggaacttact ccgtttcctt gttgagagct      840

tactccttgt tcttcgttat cggtactgct ttggctattt gtgttccacc agttgagtgg      900

actccattca gatccttgga gcagttgact gctttgttcg ttttcgtttt catgtgggct      960

ttgcactact ctgagtactt gagagagaga gctagagcac caattcactc ctccaaggct     1020

ttgcaaatca gagctagaat cttcatggga actttgtcct tgttgttgat cgttgctatc     1080

tacttgttct ccactggtta cttcagatcc ttctcatcca gagttagagc tttgtttgtt     1140

aagcacacta gaactggtaa cccattggtt gactccgttg ctgaacacag accaactact     1200

gctggtgctt tcttgagaca cttgcatgtt tgttacaatg gatggatcat cggttttttc     1260

ttcatgtccg tttcttgttt cttccactgt actccaggaa tgtccttctt gttgttgtac     1320

tccatcttgg cttactactt ctcattgaag atgtccagat tgttgttgtt gtccgctcca     1380

gttgcttcta tcttgactgg ttacgttgtt ggttccatcg ttgatttggc tgctgattgt     1440

ttcgctgctt ctggtactga acacgctgac tccaaagaac accagggtaa agctagagga     1500

aagggacaga agagacagat cactgttgag tgtggttgtc acaacccatt ctacaagttg     1560

tggtgtaact cattctcctc cagattggtt gttggaaagt tcttcgttgt tgttgttttg     1620

tccatctgtg gtccaacttt cttgggttcc gagttcagag cacactgtga gagattctcc     1680

gtttccgttg ctaacccaag aatcatctcc tccatcagac actctggtaa gttggttttg     1740

gctgacgact actacgtttc ctacttgtgg ttgagaaaca acactccaga ggacgctaga     1800

attttgtctt ggtgggacta cggttaccaa atcactggta tcggtaacag aactactttg     1860

gctgacggta acacttggaa ccacgagcac attgctacta tcggaaagat gttgacttcc     1920

ccagttaaag agtcccacgc tttgattaga cacttggctg actacgtttt gatttgggct     1980

ggtgaagata gaggagactt gagaaagtcc agacacatgg ctagaatcgg taactccgtt     2040

tacagagaca tgtgttctga ggacgaccca ttgtgtactc agttcggttt ctactccggt     2100

gatttcaaca agccaactcc aatgatgcag agatccttgt tgtacaactt gcacagattc     2160

ggtactgatg gtggaaagac tcagttggac aagaacatgt tccagttggc ttacgtttcc     2220

aagtacggat tggtcaaaat ctacaaggtt atgaacgttt ccgaagagtc taaggcttgg     2280

gttgcagacc caaagaatag aaagtgtgac gctcctggtt cttggatttg tgctggtcaa     2340

tacccacctg ctaaagaaat ccaggacatg ttggctaaga gaatcgacta cgagcaattg     2400

gaggacttca acagaagaaa tagatccgac gcttactaca gagcttacat gagacagatg     2460

ggttaatag                                                             2469


<210>  15
<211>  1350
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Anti-Her2 Heavy chain (VH + IgG1 constant region)

<400>  15
gaggttcagt tggttgaatc tggaggagga ttggttcaac ctggtggttc tttgagattg       60

tcctgtgctg cttccggttt caacatcaag gacacttaca tccactgggt tagacaagct      120

ccaggaaagg gattggagtg ggttgctaga atctacccaa ctaacggtta cacaagatac      180

gctgactccg ttaagggaag attcactatc tctgctgaca cttccaagaa cactgcttac      240

ttgcagatga actccttgag agctgaggat actgctgttt actactgttc cagatggggt      300

ggtgatggtt tctacgctat ggactactgg ggtcaaggaa ctttggttac tgtttcctcc      360

gcttctacta agggaccatc tgttttccca ttggctccat cttctaagtc tacttccggt      420

ggtactgctg ctttgggatg tttggttaaa gactacttcc cagagccagt tactgtttct      480

tggaactccg gtgctttgac ttctggtgtt cacactttcc cagctgtttt gcaatcttcc      540

ggtttgtact ctttgtcctc cgttgttact gttccatcct cttccttggg tactcagact      600

tacatctgta acgttaacca caagccatcc aacactaagg ttgacaagaa ggttgagcca      660

aagtcctgtg acaagacaca tacttgtcca ccatgtccag ctccagaatt gttgggtggt      720

ccatccgttt tcttgttccc accaaagcca aaggacactt tgatgatctc cagaactcca      780

gaggttacat gtgttgttgt tgacgtttct cacgaggacc cagaggttaa gttcaactgg      840

tacgttgacg gtgttgaagt tcacaacgct aagactaagc caagagaaga gcagtacaac      900

tccacttaca gagttgtttc cgttttgact gttttgcacc aggactggtt gaacggtaaa      960

gaatacaagt gtaaggtttc caacaaggct ttgccagctc caatcgaaaa gactatctcc     1020

aaggctaagg gtcaaccaag agagccacag gtttacactt tgccaccatc cagagaagag     1080

atgactaaga accaggtttc cttgacttgt ttggttaaag gattctaccc atccgacatt     1140

gctgttgagt gggaatctaa cggtcaacca gagaacaact acaagactac tccaccagtt     1200

ttggattctg atggttcctt cttcttgtac tccaagttga ctgttgacaa gtccagatgg     1260

caacagggta acgttttctc ctgttccgtt atgcatgagg ctttgcacaa ccactacact     1320

caaaagtcct tgtctttgtc ccctggttaa                                      1350


<210>  16
<211>  645
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Anti-Her2 light chain (VL + Kappa constant region)

<400>  16
gacatccaaa tgactcaatc cccatcttct ttgtctgctt ccgttggtga cagagttact       60

atcacttgta gagcttccca ggacgttaat actgctgttg cttggtatca acagaagcca      120

ggaaaggctc caaagttgtt gatctactcc gcttccttct tgtactctgg tgttccatcc      180

agattctctg gttccagatc cggtactgac ttcactttga ctatctcctc cttgcaacca      240

gaagatttcg ctacttacta ctgtcagcag cactacacta ctccaccaac tttcggacag      300

ggtactaagg ttgagatcaa gagaactgtt gctgctccat ccgttttcat tttcccacca      360

tccgacgaac agttgaagtc tggtacagct tccgttgttt gtttgttgaa caacttctac      420

ccaagagagg ctaaggttca gtggaaggtt gacaacgctt tgcaatccgg taactcccaa      480

gaatccgtta ctgagcaaga ctctaaggac tccacttact ccttgtcctc cactttgact      540

ttgtccaagg ctgattacga gaagcacaag gtttacgctt gtgaggttac acatcagggt      600

ttgtcctccc cagttactaa gtccttcaac agaggagagt gttaa                      645


<210>  17
<211>  260
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PpAOX1 TT

<400>  17
tcaagaggat gtcagaatgc catttgcctg agagatgcag gcttcatttt gatacttttt       60

tatttgtaac ctatatagta taggattttt tttgtcattt tgtttcttct cgtacgagct      120

tgctcctgat cagcctatct cgcagctgat gaatatcttg tggtaggggt ttgggaaaat      180

cattcgagtt tgatgttttt cttggtattt cccactcctc ttcagagtac agaagattaa      240

gtgagacgtt cgtttgtgca                                                  260


<210>  18
<211>  375
<212>  DNA
<213>  Artificial

<220>
<223>  Sh ble ORF (Zeocin resistance marker)

<400>  18
atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc       60

gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt      120

gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac      180

aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag      240

gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag      300

ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc      360

gaggagcagg actga                                                       375


<210>  19
<211>  1430
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of the 5'-Region used for knock out of YOS9

<400>  19
ccatagcctc tgattgatgt aagcaccgac agtacctggc tctaacttgt tagaggtttt       60

ggtggtcaag acatatctgt tatcacaaat aacataatgg ttatcgggaa agtcattggg      120

atgaacagca agtgtgttca tgatggcaaa ttcattaccc ggagagttga ctatcttcaa      180

tacatgcacc tttggagcat ttctctttgt gaatcccagt ttttccatgg ttgtggcaaa      240

gtgtagagat gttaagtgca gcgagcaaag acaagtagat agactgtatg gtgttctgat      300

gttatagttg tagtgaataa tctataaatg ccttatttga aggtttatgt aatagattta      360

cccgtgtgta gcaagtgtac tgctaagagg tactataaag ttattcatgt ggatatattc      420

agtagataat aacaaagcta caaggagatc aagaaaccat atgagttgtt cgtcacataa      480

gagattacgt aatgacaaat cggggaacta gtaccaattc tgtcttaaag tagtgtctct      540

ctaagcataa cgacctattt gataactggg ctgaactcca agcagcctga tgatgttgac      600

ctgacttatt cagaagggct attggttttg atttccagat attagcataa ttagcaatgc      660

cggaacaata tacatccaat atttttgaat gaatgaacgg ttatcaacat ttacttctgc      720

ctcctcgtct atgacttcct tgagttccag cttgttatcg gatctgattt ttttgatttt      780

cttttctttt cttggtagtt tgggaattgg tgcctgtcga atttgttcaa ctattaggtt      840

aagacctttc tgactagcat cgaagaaggc tacattttcg atgtcgttgt gtttgttgat      900

agtcagcttg atatcctgtg caattggaga acttagtctt ttgtaattga agcagccttc      960

gtccaaacat attctgtaaa gatcacttgg caggtctagt tgttcaccgg tgtgcaattt     1020

ccattttgag tcaaattcta gtgtggccaa gttgaacgag ttctgagcga aatcaatagc     1080

cttcaactga tacgcaaatg tagaccccaa gaaaagaaac aacgtgacga ggctttgtag     1140

ggtagtagcc attgtcgaat agttgaggat aagtagacgg cgagttattc tccttgataa     1200

atgctatcgc gatggatagt gattacagtg cgataatatt atccttttca tccacgtcaa     1260

ccatggttaa caggccattg gacattatga taaaggtcct gctattcctg ctctccctat     1320

caagtcttgt gaaagctttg gatgattcca ttgataagaa ttctgtggta agtcttttaa     1380

tttttgtttt cacaagatca tgccgtgcta actgggtact atagtatacc                1430


<210>  20
<211>  1498
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of the 3'-Region used for knock out of YOS9

<400>  20
ggttcctatt cactgaagac agaatacctc atgacactcc aaactttaga gtgtataacg       60

gagttaatgt gaattaagac aatttatata ctcagtaaaa taaatactag tacttacgtc      120

tttttttagt cagagcacta actctgctgg aagggttctt cgtgtaaatt ggtacagacg      180

ctggtaaagt accactatac gttgtttgac aaataggtag tttgaagctg acatcaagtt      240

tcaagtcctt aggagtcaca ttgcgagttt gaatgaccaa ttgtattaat ctcttaatct      300

tgaagtacaa tctcttctct ttgagactgg gtttcaagac agtgacggga ttagcaggat      360

cgattttggg tgatgcctta tacctttctt gacgtaattg tgacagatct attagcaact      420

tgcttataag ttcttgctct ttgttggaac ggatagcctc tatctcatcc tcctcaacga      480

agcttcccgg agtccaggag aggaggttgt ctagcttgat cttatagtct tcggatccat      540

tgacctggac ttccttatct gtgttttcaa gtttagttga tgtatctgtc cccgtatggc      600

cattcttagt ctcctggtca acaggtgccg gaagctcttt ttcaattctt tttggttcgt      660

ccttctgaag ttcattatcc gtctcatttt tagatggtct gctcagtttt tctgctatat      720

caccaagctt tctaaaacca gcttgctcca gccacctcag gcccttcaat tcactggaga      780

ttgcagattt ttcttcgtct attgtaggtg caaaactgaa atcgttaccc ttattgtggg      840

tgagccattg acccatcggt aacgcgtacc agttcaaatg aaagaggttt ggcaataaat      900

ccgtaggttt ggtggctggg tgaggttcat tgttgtattg aggagaaatc ttgttaagcg      960

gctgtgaact aatggaaggg acatggggga ttactttcgt cagattaaaa tcgccttcat     1020

tcactacagc ttctctagca tccaagcttg atttattatt cagggacgaa aacaatggcg     1080

cattaggtgt gatgaatgta gttaaacatt ctccgttgga tgaaacaaaa aatgtggaca     1140

ctttattgaa gtcttttgtc atcgattctt caaactcact ggtgtaatca tctaaaacac     1200

gagagtcaac gctttctctt agttgtctgt agttgaacaa aaatcttcct gcctctctga     1260

tcaataactc aaccatcgac ttgtagaaca aatcaatctt gacgtagtct tccgaatctc     1320

tgttccgttc gtttataagt atcaggcaca ctaaagttag gtcgtgaaat atggaataaa     1380

tagtcttgta gtgaccactc tttattctgt cgctgatggt aaccagctct gtaggtttga     1440

gatccttacc atcaacaagc tgatagtatg atccagctat caaggaagga tcctggac       1498


<210>  21
<211>  1699
<212>  DNA
<213>  artificial sequence

<220>
<223>  Sequence of the 5'-Region used for knock out of ALG3

<400>  21
aaccttcatg gaacgattcg gatacggaaa aacctgagat agttttaact agagtagatg       60

caagatttca cgattctaaa gaccgagaag gagatgtctg atgtcggtaa ctactatccg      120

gtaaatgata ttagcacact atatgctact agcgagtctg gaaccaattc tactatccat      180

tgatgctcta ttagggatgg agaattcaat caacccctct aattctgatt tcagatgttc      240

caacagcgaa gtagcccttg acaagttctc aacatcactc atcttagcta cattcacgta      300

tgctttgata aaaaactctc tacttttgtc aatgagctct agcctagtct ctggttctat      360

cgtttcctct ttggtctcca gattactctc tggattagaa tctacatcca tcttcatatc      420

tatgtccatg tccagctcaa ttttcatacc gtcagtattc ttagattcga tagcagtatc      480

tgatctggta gatccattag ttgctgcagc ggtattttct ttggaatttg gagcactttc      540

ctgtttctgt ttcataaaga ctcggtagat tgcaatgact atatcgtttc tgtagaactt      600

gtaaccatga gtccaaaatt gggtttcagg catgtatcct agctcatcta aatatccaac      660

cacatcatcc gtgctacata tagtagactc gtagagtgtc tgtgaagaaa cggctctttt      720

tcctgccaaa ggaacgtccg atatttgaag ggtccatata cgattttcct tattaagagc      780

ttcaagatgt ttcttattaa acaattcaaa gtcttttaat tcaattgtgt tatcaatagg      840

atcctcaacg tcctgtttcc attcggtgga cattctcatc ttgtattgtt cgatttggtt      900

gacttttcca gtctggaact caggactata aggaaacttt ggagttaaaa taacagtata      960

agttgagagc cttgcgggca ccatacccgt tagagacttc aacgtctcca agatcaactg     1020

cagttgagac tcttggattc tagataccag agacacctgt tgtaccatat aattaagtga     1080

ctgggctggc ttggatacag gatttcgaga agtgcttcga attatcagac cgaaggcagt     1140

tgatattttg tgcctcagcc ttaatgttcc ctataactta aggctataca cagctttatg     1200

attaatgaat ctgggctgct ggtgacgaat ttcgtcaatg accagttgcc tacgggcgat     1260

aattattttt tcagttggat gaaagaacgg aaaaacccgg tcagattcaa aaagaatatt     1320

gataatcttt gtctagcaca actgaaatgc ttggaaactc tcccaagcat gaatcagacc     1380

tgagattgta ttagacgaaa aaattgtagt atagagttat agacatatag gttgtggcaa     1440

tatcctgtgc aagccaatat ctcacagaaa taaacgtaca caccagatac aactatttcg     1500

aaaagcacac tttgagcgca acagtgattg tcctaacagt ataggtttct aaggccccag     1560

cagaccatga cggcaaatta tttatttccc ctcgtatttg ccttatctcc ttttgttctc     1620

attcttatct tggctactgt aattatctgg ataaccctcg atacttcgct tggtttctac     1680

ctcacaacat atccctacc                                                  1699


<210>  22
<211>  1052
<212>  DNA
<213>  artificial sequence

<220>
<223>  Sequence of the 3'-Region used for knock out of ALG3

<400>  22
atttacaatt agtaatatta aggtggtaaa aacattcgta gaattgaaat gaattaatat       60

agtatgacaa tggttcatgt ctataaatct ccggcttcgg taccttctcc ccaattgaat      120

acattgtcaa aatgaatggt tgaactatta ggttcgccag tttcgttatt aagaaaactg      180

ttaaaatcaa attccatatc atcggttcca gtgggaggac cagttccatc gccaaaatcc      240

tgtaagaatc cattgtcaga acctgtaaag tcagtttgag atgaaatttt tccggtcttt      300

gttgacttgg aagcttcgtt aaggttaggt gaaacagttt gatcaaccag cggctcccgt      360

tttcgtcgct tagtagcagc attattacca ggaatgccgc ctgtagagtt ttgatgtgtc      420

ctagctgcaa ttggagtctg tggagtagtg ggagtcgggg gctcagtagc tttctttgcc      480

ttctttttag ctggctcctt tttctttcgt acaggtgcga cattatttgg tgtagacccc      540

gcagaagtgt taccagtact atgtgcagtg ttttgagttt gtgtaccagg tgaagttccg      600

ggagtattct tcgtgaccac tgcagagttc tggggaggga gcattacatt cacattaaat      660

tttggttcgg gcggtgtgtg ctctggaatt ggatcaaagt tagaaaaatg cccgcttccc      720

ttcttacatg ccatgtcatg acgctgtttg ttctgtttct caagcatcat tagctctttc      780

tgatactcct gtatacctac aattttagaa gcacttgatt gagactgttg cgattgctgg      840

tgttggctct gtgattgtgg ttgtgctatt tgctgatgtt gtgaccctgg agttggaact      900

agctccggct gctgaataga agaaggcgga gaatgttgcg gttgagatgc aggtaaaggc      960

tgctgataaa caggaccagg ttgcgagaat ctaggtgtgg tggacgagtg aggagtaccg     1020

gcggcagaag tagagtgagg cagaggagcc at                                   1052


<210>  23
<211>  411
<212>  DNA
<213>  artificial sequence

<220>
<223>  Sequence of the 5'-Region used for knock out of  BMT1

<400>  23
catatggtga gagccgttct gcacaactag atgttttcga gcttcgcatt gtttcctgca       60

gctcgactat tgaattaaga tttccggata tctccaatct cacaaaaact tatgttgacc      120

acgtgctttc ctgaggcgag gtgttttata tgcaagctgc caaaaatgga aaacgaatgg      180

ccatttttcg cccaggcaaa ttattcgatt actgctgtca taaagacagt gttgcaaggc      240

tcacattttt ttttaggatc cgagataaag tgaatacagg acagcttatc tctatatctt      300

gtaccattcg tgaatcttaa gagttcggtt agggggactc tagttgaggg ttggcactca      360

cgtatggctg ggcgcagaaa taaaattcag gcgcagcagc acttatcgat g               411


<210>  24
<211>  692
<212>  DNA
<213>  artificial sequence

<220>
<223>  Sequence of the 3'-Region used for knock out of BMT1

<400>  24
gaattcacag ttataaataa aaacaaaaac tcaaaaagtt tgggctccac aaaataactt       60

aatttaaatt tttgtctaat aaatgaatgt aattccaaga ttatgtgatg caagcacagt      120

atgcttcagc cctatgcagc tactaatgtc aatctcgcct gcgagcgggc ctagattttc      180

actacaaatt tcaaaactac gcggatttat tgtctcagag agcaatttgg catttctgag      240

cgtagcagga ggcttcataa gattgtatag gaccgtacca acaaattgcc gaggcacaac      300

acggtatgct gtgcacttat gtggctactt ccctacaacg gaatgaaacc ttcctctttc      360

cgcttaaacg agaaagtgtg tcgcaattga atgcaggtgc ctgtgcgcct tggtgtattg      420

tttttgaggg cccaatttat caggcgcctt ttttcttggt tgttttccct tagcctcaag      480

caaggttggt ctatttcatc tccgcttcta taccgtgcct gatactgttg gatgagaaca      540

cgactcaact tcctgctgct ctgtattgcc agtgttttgt ctgtgatttg gatcggagtc      600

ctccttactt ggaatgataa taatcttggc ggaatctccc taaacggagg caaggattct      660

gcctatgatg atctgctatc attgggaagc tt                                    692


<210>  25
<211>  1043
<212>  DNA
<213>  artificial sequence

<220>
<223>  Sequence of the 5'-Region used for knock out of BMT4

<400>  25
aagcttgttc accgttggga cttttccgtg gacaatgttg actactccag gagggattcc       60

agctttctct actagctcag caataatcaa tgcagcccca ggcgcccgtt ctgatggctt      120

gatgaccgtt gtattgcctg tcactatagc caggggtagg gtccataaag gaatcatagc      180

agggaaatta aaagggcata ttgatgcaat cactcccaat ggctctcttg ccattgaagt      240

ctccatatca gcactaactt ccaagaagga ccccttcaag tctgacgtga tagagcacgc      300

ttgctctgcc acctgtagtc ctctcaaaac gtcaccttgt gcatcagcaa agactttacc      360

ttgctccaat actatgacgg aggcaattct gtcaaaattc tctctcagca attcaaccaa      420

cttgaaagca aattgctgtc tcttgatgat ggagactttt ttccaagatt gaaatgcaat      480

gtgggacgac tcaattgctt cttccagctc ctcttcggtt gattgaggaa cttttgaaac      540

cacaaaattg gtcgttgggt catgtacatc aaaccattct gtagatttag attcgacgaa      600

agcgttgttg atgaaggaaa aggttggata cggtttgtcg gtctctttgg tatggccggt      660

ggggtatgca attgcagtag aagataattg gacagccatt gttgaaggta gagaaaaggt      720

cagggaactt gggggttatt tataccattt taccccacaa ataacaactg aaaagtaccc      780

attccatagt gagaggtaac cgacggaaaa agacgggccc atgttctggg accaatagaa      840

ctgtgtaatc cattgggact aatcaacaga cgattggcaa tataatgaaa tagttcgttg      900

aaaagccacg tcagctgtct tttcattaac tttggtcgga cacaacattt tctactgttg      960

tatctgtcct actttgctta tcatctgcca cagggcaagt ggatttcctt ctcgcgcggc     1020

tgggtgaaaa cggttaacgt gaa                                             1043


<210>  26
<211>  695
<212>  DNA
<213>  artificial sequence

<220>
<223>  Sequence of the 3'-Region used for knock out of BMT4

<400>  26
gccttggggg acttcaagtc tttgctagaa actagatgag gtcaggccct cttatggttg       60

tgtcccaatt gggcaatttc actcacctaa aaagcatgac aattatttag cgaaataggt      120

agtatatttt ccctcatctc ccaagcagtt tcgtttttgc atccatatct ctcaaatgag      180

cagctacgac tcattagaac cagagtcaag taggggtgag ctcagtcatc agccttcgtt      240

tctaaaacga ttgagttctt ttgttgctac aggaagcgcc ctagggaact ttcgcacttt      300

ggaaatagat tttgatgacc aagagcggga gttgatatta gagaggctgt ccaaagtaca      360

tgggatcagg ccggccaaat tgattggtgt gactaaacca ttgtgtactt ggacactcta      420

ttacaaaagc gaagatgatt tgaagtatta caagtcccga agtgttagag gattctatcg      480

agcccagaat gaaatcatca accgttatca gcagattgat aaactcttgg aaagcggtat      540

cccattttca ttattgaaga actacgataa tgaagatgtg agagacggcg accctctgaa      600

cgtagacgaa gaaacaaatc tacttttggg gtacaataga gaaagtgaat caagggaggt      660

atttgtggcc ataatactca actctatcat taatg                                 695


<210>  27
<211>  546
<212>  DNA
<213>  artificial sequence

<220>
<223>  Sequence of the 5'-Region used for knock out of BMT3

<400>  27
gatatctccc tggggacaat atgtgttgca actgttcgtt gttggtgccc cagtccccca       60

accggtacta atcggtctat gttcccgtaa ctcatattcg gttagaacta gaacaataag      120

tgcatcattg ttcaacattg tggttcaatt gtcgaacatt gctggtgctt atatctacag      180

ggaagacgat aagcctttgt acaagagagg taacagacag ttaattggta tttctttggg      240

agtcgttgcc ctctacgttg tctccaagac atactacatt ctgagaaaca gatggaagac      300

tcaaaaatgg gagaagctta gtgaagaaga gaaagttgcc tacttggaca gagctgagaa      360

ggagaacctg ggttctaaga ggctggactt tttgttcgag agttaaactg cataattttt      420

tctaagtaaa tttcatagtt atgaaatttc tgcagcttag tgtttactgc atcgtttact      480

gcatcaccct gtaaataatg tgagcttttt tccttccatt gcttggtatc ttccttgctg      540

ctgttt                                                                 546


<210>  28
<211>  378
<212>  DNA
<213>  artificial sequence

<220>
<223>  Sequence of the 3'-Region used for knock out of BMT3

<400>  28
acaaaacagt catgtacaga actaacgcct ttaagatgca gaccactgaa aagaattggg       60

tcccattttt cttgaaagac gaccaggaat ctgtccattt tgtttactcg ttcaatcctc      120

tgagagtact caactgcagt cttgataacg gtgcatgtga tgttctattt gagttaccac      180

atgattttgg catgtcttcc gagctacgtg gtgccactcc tatgctcaat cttcctcagg      240

caatcccgat ggcagacgac aaagaaattt gggtttcatt cccaagaacg agaatatcag      300

attgcgggtg ttctgaaaca atgtacaggc caatgttaat gctttttgtt agagaaggaa      360

caaacttttt tgctgagc                                                    378


<210>  29
<211>  1494
<212>  DNA
<213>  artificial sequence

<220>
<223>  DNA encodes Tr ManI catalytic domain

<400>  29
cgcgccggat ctcccaaccc tacgagggcg gcagcagtca aggccgcatt ccagacgtcg       60

tggaacgctt accaccattt tgcctttccc catgacgacc tccacccggt cagcaacagc      120

tttgatgatg agagaaacgg ctggggctcg tcggcaatcg atggcttgga cacggctatc      180

ctcatggggg atgccgacat tgtgaacacg atccttcagt atgtaccgca gatcaacttc      240

accacgactg cggttgccaa ccaaggcatc tccgtgttcg agaccaacat tcggtacctc      300

ggtggcctgc tttctgccta tgacctgttg cgaggtcctt tcagctcctt ggcgacaaac      360

cagaccctgg taaacagcct tctgaggcag gctcaaacac tggccaacgg cctcaaggtt      420

gcgttcacca ctcccagcgg tgtcccggac cctaccgtct tcttcaaccc tactgtccgg      480

agaagtggtg catctagcaa caacgtcgct gaaattggaa gcctggtgct cgagtggaca      540

cggttgagcg acctgacggg aaacccgcag tatgcccagc ttgcgcagaa gggcgagtcg      600

tatctcctga atccaaaggg aagcccggag gcatggcctg gcctgattgg aacgtttgtc      660

agcacgagca acggtacctt tcaggatagc agcggcagct ggtccggcct catggacagc      720

ttctacgagt acctgatcaa gatgtacctg tacgacccgg ttgcgtttgc acactacaag      780

gatcgctggg tccttgctgc cgactcgacc attgcgcatc tcgcctctca cccgtcgacg      840

cgcaaggact tgaccttttt gtcttcgtac aacggacagt ctacgtcgcc aaactcagga      900

catttggcca gttttgccgg tggcaacttc atcttgggag gcattctcct gaacgagcaa      960

aagtacattg actttggaat caagcttgcc agctcgtact ttgccacgta caaccagacg     1020

gcttctggaa tcggccccga aggcttcgcg tgggtggaca gcgtgacggg cgccggcggc     1080

tcgccgccct cgtcccagtc cgggttctac tcgtcggcag gattctgggt gacggcaccg     1140

tattacatcc tgcggccgga gacgctggag agcttgtact acgcataccg cgtcacgggc     1200

gactccaagt ggcaggacct ggcgtgggaa gcgttcagtg ccattgagga cgcatgccgc     1260

gccggcagcg cgtactcgtc catcaacgac gtgacgcagg ccaacggcgg gggtgcctct     1320

gacgatatgg agagcttctg gtttgccgag gcgctcaagt atgcgtacct gatctttgcg     1380

gaggagtcgg atgtgcaggt gcaggccaac ggcgggaaca aatttgtctt taacacggag     1440

gcgcacccct ttagcatccg ttcatcatca cgacggggcg gccaccttgc ttaa           1494


<210>  30
<211>  1231
<212>  DNA
<213>  artificial sequence

<220>
<223>  Sequence of the 5'-region that was used to knock into the PpPRO1 
       locus

<400>  30
gaagggccat cgaattgtca tcgtctcctc aggtgccatc gctgtgggca tgaagagagt       60

caacatgaag cggaaaccaa aaaagttaca gcaagtgcag gcattggctg ctataggaca      120

aggccgtttg ataggacttt gggacgacct tttccgtcag ttgaatcagc ctattgcgca      180

gattttactg actagaacgg atttggtcga ttacacccag tttaagaacg ctgaaaatac      240

attggaacag cttattaaaa tgggtattat tcctattgtc aatgagaatg acaccctatc      300

cattcaagaa atcaaatttg gtgacaatga caccttatcc gccataacag ctggtatgtg      360

tcatgcagac tacctgtttt tggtgactga tgtggactgt ctttacacgg ataaccctcg      420

tacgaatccg gacgctgagc caatcgtgtt agttagaaat atgaggaatc taaacgtcaa      480

taccgaaagt ggaggttccg ccgtaggaac aggaggaatg acaactaaat tgatcgcagc      540

tgatttgggt gtatctgcag gtgttacaac gattatttgc aaaagtgaac atcccgagca      600

gattttggac attgtagagt acagtatccg tgctgataga gtcgaaaatg aggctaaata      660

tctggtcatc aacgaagagg aaactgtgga acaatttcaa gagatcaatc ggtcagaact      720

gagggagttg aacaagctgg acattccttt gcatacacgt ttcgttggcc acagttttaa      780

tgctgttaat aacaaagagt tttggttact ccatggacta aaggccaacg gagccattat      840

cattgatcca ggttgttata aggctatcac tagaaaaaac aaagctggta ttcttccagc      900

tggaattatt tccgtagagg gtaatttcca tgaatacgag tgtgttgatg ttaaggtagg      960

actaagagat ccagatgacc cacattcact agaccccaat gaagaacttt acgtcgttgg     1020

ccgtgcccgt tgtaattacc ccagcaatca aatcaacaaa attaagggtc tacaaagctc     1080

gcagatcgag caggttctag gttacgctga cggtgagtat gttgttcaca gggacaactt     1140

ggctttccca gtatttgccg atccagaact gttggatgtt gttgagagta ccctgtctga     1200

acaggagaga gaatccaaac caaataaata g                                    1231


<210>  31
<211>  1425
<212>  DNA
<213>  artificial sequence

<220>
<223>  Sequence of the 3'-region that was used to knock into the PpPRO1 
       locus

<400>  31
aatttcacat atgctgcttg attatgtaat tataccttgc gttcgatggc atcgatttcc       60

tcttctgtca atcgcgcatc gcattaaaag tatacttttt tttttttcct atagtactat      120

tcgccttatt ataaactttg ctagtatgag ttctaccccc aagaaagagc ctgatttgac      180

tcctaagaag agtcagcctc caaagaatag tctcggtggg ggtaaaggct ttagtgagga      240

gggtttctcc caaggggact tcagcgctaa gcatatacta aatcgtcgcc ctaacaccga      300

aggctcttct gtggcttcga acgtcatcag ttcgtcatca ttgcaaaggt taccatcctc      360

tggatctgga agcgttgctg tgggaagtgt gttgggatct tcgccattaa ctctttctgg      420

agggttccac gggcttgatc caaccaagaa taaaatagac gttccaaagt cgaaacagtc      480

aaggagacaa agtgttcttt ctgacatgat ttccacttct catgcagcta gaaatgatca      540

ctcagagcag cagttacaaa ctggacaaca atcagaacaa aaagaagaag atggtagtcg      600

atcttctttt tctgtttctt cccccgcaag agatatccgg cacccagatg tactgaaaac      660

tgtcgagaaa catcttgcca atgacagcga gatcgactca tctttacaac ttcaaggtgg      720

agatgtcact agaggcattt atcaatgggt aactggagaa agtagtcaaa aagataaccc      780

gcctttgaaa cgagcaaata gttttaatga tttttcttct gtgcatggtg acgaggtagg      840

caaggcagat gctgaccacg atcgtgaaag cgtattcgac gaggatgata tctccattga      900

tgatatcaaa gttccgggag ggatgcgtcg aagtttttta ttacaaaagc atagagacca      960

acaactttct ggactgaata aaacggctca ccaaccaaaa caacttacta aacctaattt     1020

cttcacgaac aactttatag agtttttggc attgtatggg cattttgcag gtgaagattt     1080

ggaggaagac gaagatgaag atttagacag tggttccgaa tcagtcgcag tcagtgatag     1140

tgagggagaa ttcagtgagg ctgacaacaa tttgttgtat gatgaagagt ctctcctatt     1200

agcacctagt acctccaact atgcgagatc aagaatagga agtattcgta ctcctactta     1260

tggatctttc agttcaaatg ttggttcttc gtctattcat cagcagttaa tgaaaagtca     1320

aatcccgaag ctgaagaaac gtggacagca caagcataaa acacaatcaa aaatacgctc     1380

gaagaagcaa actaccaccg taaaagcagt gttgctgcta ttaaa                     1425


<210>  32
<211>  1350
<212>  DNA
<213>  artificial sequence

<220>
<223>  Anti-Her2 Heavy chain (VH + IgG1 constant region)

<400>  32
gaggttcagt tggttgaatc tggaggagga ttggttcaac ctggtggttc tttgagattg       60

tcctgtgctg cttccggttt caacatcaag gacacttaca tccactgggt tagacaagct      120

ccaggaaagg gattggagtg ggttgctaga atctacccaa ctaacggtta cacaagatac      180

gctgactccg ttaagggaag attcactatc tctgctgaca cttccaagaa cactgcttac      240

ttgcagatga actccttgag agctgaggat actgctgttt actactgttc cagatggggt      300

ggtgatggtt tctacgctat ggactactgg ggtcaaggaa ctttggttac tgtttcctcc      360

gcttctacta agggaccatc tgttttccca ttggctccat cttctaagtc tacttccggt      420

ggtactgctg ctttgggatg tttggttaaa gactacttcc cagagccagt tactgtttct      480

tggaactccg gtgctttgac ttctggtgtt cacactttcc cagctgtttt gcaatcttcc      540

ggtttgtact ctttgtcctc cgttgttact gttccatcct cttccttggg tactcagact      600

tacatctgta acgttaacca caagccatcc aacactaagg ttgacaagaa ggttgagcca      660

aagtcctgtg acaagacaca tacttgtcca ccatgtccag ctccagaatt gttgggtggt      720

ccatccgttt tcttgttccc accaaagcca aaggacactt tgatgatctc cagaactcca      780

gaggttacat gtgttgttgt tgacgtttct cacgaggacc cagaggttaa gttcaactgg      840

tacgttgacg gtgttgaagt tcacaacgct aagactaagc caagagaaga gcagtacaac      900

tccacttaca gagttgtttc cgttttgact gttttgcacc aggactggtt gaacggtaaa      960

gaatacaagt gtaaggtttc caacaaggct ttgccagctc caatcgaaaa gactatctcc     1020

aaggctaagg gtcaaccaag agagccacag gtttacactt tgccaccatc cagagaagag     1080

atgactaaga accaggtttc cttgacttgt ttggttaaag gattctaccc atccgacatt     1140

gctgttgagt gggaatctaa cggtcaacca gagaacaact acaagactac tccaccagtt     1200

ttggattctg atggttcctt cttcttgtac tccaagttga ctgttgacaa gtccagatgg     1260

caacagggta acgttttctc ctgttccgtt atgcatgagg ctttgcacaa ccactacact     1320

caaaagtcct tgtctttgtc ccctggttaa                                      1350


<210>  33
<211>  57
<212>  DNA
<213>  artificial sequence

<220>
<223>  Saccharomyces cerevisiae mating factor pre-signal peptide

<400>  33
atgagattcc catccatctt cactgctgtt ttgttcgctg cttcttctgc tttggct          57


<210>  34
<211>  414
<212>  DNA
<213>  artificial sequence

<220>
<223>  PpCITI TT

<400>  34
ccggccattt aaatatgtga cgactgggtg atccgggtta gtgagttgtt ctcccatctg       60

tatatttttc atttacgatg aatacgaaat gagtattaag aaatcaggcg tagcaatatg      120

ggcagtgttc agtcctgtca tagatggcaa gcactggcac atccttaata ggttagagaa      180

aatcattgaa tcatttgggt ggtgaaaaaa aattgatgta aacaagccac ccacgctggg      240

agtcgaaccc agaatctttt gattagaagt caaacgcgtt aaccattacg ctacgcaggc      300

atgtttcacg tccatttttg attgctttct atcataatct aaagatgtga actcaattag      360

ttgcaatttg accaattctt ccattacaag tcgtgcttcc tccgttgatg caac            414


<210>  35
<211>  645
<212>  DNA
<213>  artificial sequence

<220>
<223>  Anti-Her2 light chain (VL + Kappa constant region)

<400>  35
gacatccaaa tgactcaatc cccatcttct ttgtctgctt ccgttggtga cagagttact       60

atcacttgta gagcttccca ggacgttaat actgctgttg cttggtatca acagaagcca      120

ggaaaggctc caaagttgtt gatctactcc gcttccttct tgtactctgg tgttccatcc      180

agattctctg gttccagatc cggtactgac ttcactttga ctatctcctc cttgcaacca      240

gaagatttcg ctacttacta ctgtcagcag cactacacta ctccaccaac tttcggacag      300

ggtactaagg ttgagatcaa gagaactgtt gctgctccat ccgttttcat tttcccacca      360

tccgacgaac agttgaagtc tggtacagct tccgttgttt gtttgttgaa caacttctac      420

ccaagagagg ctaaggttca gtggaaggtt gacaacgctt tgcaatccgg taactcccaa      480

gaatccgtta ctgagcaaga ctctaaggac tccacttact ccttgtcctc cactttgact      540

ttgtccaagg ctgattacga gaagcacaag gtttacgctt gtgaggttac acatcagggt      600

ttgtcctccc cagttactaa gtccttcaac agaggagagt gttaa                      645


<210>  36
<211>  427
<212>  DNA
<213>  artificial sequence

<220>
<223>  ScTEF1 promoter

<400>  36
gatcccccac acaccatagc ttcaaaatgt ttctactcct tttttactct tccagatttt       60

ctcggactcc gcgcatcgcc gtaccacttc aaaacaccca agcacagcat actaaatttc      120

ccctctttct tcctctaggg tgtcgttaat tacccgtact aaaggtttgg aaaagaaaaa      180

agagaccgcc tcgtttcttt ttcttcgtcg aaaaaggcaa taaaaatttt tatcacgttt      240

ctttttcttg aaaatttttt tttttgattt ttttctcttt cgatgacctc ccattgatat      300

ttaagttaat aaacggtctt caatttctca agtttcagtt tcatttttct tgttctatta      360

caactttttt tacttcttgc tcattagaaa gaaagcatag caatctaatc taagttttaa      420

ttacaaa                                                                427


<210>  37
<211>  1793
<212>  DNA
<213>  artificial sequence

<220>
<223>  Sequence of the PpTRP2 gene integration locus

<400>  37
ggtttctcaa ttactatata ctactaacca tttacctgta gcgtatttct tttccctctt       60

cgcgaaagct caagggcatc ttcttgactc atgaaaaata tctggatttc ttctgacaga      120

tcatcaccct tgagcccaac tctctagcct atgagtgtaa gtgatagtca tcttgcaaca      180

gattattttg gaacgcaact aacaaagcag atacaccctt cagcagaatc ctttctggat      240

attgtgaaga atgatcgcca aagtcacagt cctgagacag ttcctaatct ttaccccatt      300

tacaagttca tccaatcaga cttcttaacg cctcatctgg cttatatcaa gcttaccaac      360

agttcagaaa ctcccagtcc aagtttcttg cttgaaagtg cgaagaatgg tgacaccgtt      420

gacaggtaca cctttatggg acattccccc agaaaaataa tcaagactgg gcctttagag      480

ggtgctgaag ttgacccctt ggtgcttctg gaaaaagaac tgaagggcac cagacaagcg      540

caacttcctg gtattcctcg tctaagtggt ggtgccatag gatacatctc gtacgattgt      600

attaagtact ttgaaccaaa aactgaaaga aaactgaaag atgttttgca acttccggaa      660

gcagctttga tgttgttcga cacgatcgtg gcttttgaca atgtttatca aagattccag      720

gtaattggaa acgtttctct atccgttgat gactcggacg aagctattct tgagaaatat      780

tataagacaa gagaagaagt ggaaaagatc agtaaagtgg tatttgacaa taaaactgtt      840

ccctactatg aacagaaaga tattattcaa ggccaaacgt tcacctctaa tattggtcag      900

gaagggtatg aaaaccatgt tcgcaagctg aaagaacata ttctgaaagg agacatcttc      960

caagctgttc cctctcaaag ggtagccagg ccgacctcat tgcacccttt caacatctat     1020

cgtcatttga gaactgtcaa tccttctcca tacatgttct atattgacta tctagacttc     1080

caagttgttg gtgcttcacc tgaattacta gttaaatccg acaacaacaa caaaatcatc     1140

acacatccta ttgctggaac tcttcccaga ggtaaaacta tcgaagagga cgacaattat     1200

gctaagcaat tgaagtcgtc tttgaaagac agggccgagc acgtcatgct ggtagatttg     1260

gccagaaatg atattaaccg tgtgtgtgag cccaccagta ccacggttga tcgtttattg     1320

actgtggaga gattttctca tgtgatgcat cttgtgtcag aagtcagtgg aacattgaga     1380

ccaaacaaga ctcgcttcga tgctttcaga tccattttcc cagcaggaac cgtctccggt     1440

gctccgaagg taagagcaat gcaactcata ggagaattgg aaggagaaaa gagaggtgtt     1500

tatgcggggg ccgtaggaca ctggtcgtac gatggaaaat cgatggacac atgtattgcc     1560

ttaagaacaa tggtcgtcaa ggacggtgtc gcttaccttc aagccggagg tggaattgtc     1620

tacgattctg acccctatga cgagtacatc gaaaccatga acaaaatgag atccaacaat     1680

aacaccatct tggaggctga gaaaatctgg accgataggt tggccagaga cgagaatcaa     1740

agtgaatccg aagaaaacga tcaatgaacg gaggacgtaa gtaggaattt atg            1793


<210>  38
<211>  957
<212>  DNA
<213>  artificial sequence

<220>
<223>  Sequence of the PpURA5 auxotrophic marker

<400>  38
tctagaggga cttatctggg tccagacgat gtgtatcaaa agacaaatta gagtatttat       60

aaagttatgt aagcaaatag gggctaatag ggaaagaaaa attttggttc tttatcagag      120

ctggctcgcg cgcagtgttt ttcgtgctcc tttgtaatag tcatttttga ctactgttca      180

gattgaaatc acattgaaga tgtcactgga ggggtaccaa aaaaggtttt tggatgctgc      240

agtggcttcg caggccttga agtttggaac tttcaccttg aaaagtggaa gacagtctcc      300

atacttcttt aacatgggtc ttttcaacaa agctccatta gtgagtcagc tggctgaatc      360

ttatgctcag gccatcatta acagcaacct ggagatagac gttgtatttg gaccagctta      420

taaaggtatt cctttggctg ctattaccgt gttgaagttg tacgagctgg gcggcaaaaa      480

atacgaaaat gtcggatatg cgttcaatag aaaagaaaag aaagaccacg gagaaggtgg      540

aagcatcgtt ggagaaagtc taaagaataa aagagtactg attatcgatg atgtgatgac      600

tgcaggtact gctatcaacg aagcatttgc tataattgga gctgaaggtg ggagagttga      660

aggttgtatt attgccctag atagaatgga gactacagga gatgactcaa ataccagtgc      720

tacccaggct gttagtcaga gatatggtac ccctgtcttg agtatagtga cattggacca      780

tattgtggcc catttgggcg aaactttcac agcagacgag aaatctcaaa tggaaacgta      840

tagaaaaaag tatttgccca aataagtatg aatctgcttc gaatgaatga attaatccaa      900

ttatcttctc accattattt tcttctgttt cggagctttg ggcacggcgg cggatcc         957


<210>  39
<211>  709
<212>  DNA
<213>  artificial sequence

<220>
<223>  Sequence of the part of the Ec lacZ gene that was used to 
       construct the PpURA5 blaster (recyclable auxotrophic marker)

<400>  39
cctgcactgg atggtggcgc tggatggtaa gccgctggca agcggtgaag tgcctctgga       60

tgtcgctcca caaggtaaac agttgattga actgcctgaa ctaccgcagc cggagagcgc      120

cgggcaactc tggctcacag tacgcgtagt gcaaccgaac gcgaccgcat ggtcagaagc      180

cgggcacatc agcgcctggc agcagtggcg tctggcggaa aacctcagtg tgacgctccc      240

cgccgcgtcc cacgccatcc cgcatctgac caccagcgaa atggattttt gcatcgagct      300

gggtaataag cgttggcaat ttaaccgcca gtcaggcttt ctttcacaga tgtggattgg      360

cgataaaaaa caactgctga cgccgctgcg cgatcagttc acccgtgcac cgctggataa      420

cgacattggc gtaagtgaag cgacccgcat tgaccctaac gcctgggtcg aacgctggaa      480

ggcggcgggc cattaccagg ccgaagcagc gttgttgcag tgcacggcag atacacttgc      540

tgatgcggtg ctgattacga ccgctcacgc gtggcagcat caggggaaaa ccttatttat      600

cagccggaaa acctaccgga ttgatggtag tggtcaaatg gcgattaccg ttgatgttga      660

agtggcgagc gatacaccgc atccggcgcg gattggcctg aactgccag                  709


<210>  40
<211>  542
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  40

Met Gln Ala Lys Ile Ile Tyr Ala Leu Ser Ala Ile Ser Ala Leu Ile 
1               5                   10                  15      


Pro Leu Gly Ser Ser Leu Leu Ala Pro Ile Glu Asp Pro Ile Val Ser 
            20                  25                  30          


Asn Lys Tyr Leu Ile Ser Tyr Ile Asp Glu Asp Asp Trp Ser Asp Arg 
        35                  40                  45              


Ile Leu Gln Asn Gln Ser Val Met Asn Ser Gly Tyr Ile Val Asn Met 
    50                  55                  60                  


Gly Asp Asp Leu Glu Cys Phe Ile Gln Asn Ala Ser Thr Gln Leu Asn 
65                  70                  75                  80  


Asp Val Leu Glu Asp Ser Asn Glu His Ser Asn Ser Glu Lys Thr Ala 
                85                  90                  95      


Leu Leu Thr Lys Thr Leu Asn Gln Gly Val Lys Thr Ile Phe Asp Lys 
            100                 105                 110         


Leu Asn Glu Arg Cys Ile Phe Tyr Gln Ala Gly Phe Trp Ile Tyr Glu 
        115                 120                 125             


Tyr Cys Pro Gly Ile Glu Phe Val Gln Phe His Gly Arg Val Asn Thr 
    130                 135                 140                 


Lys Thr Gly Glu Ile Val Asn Arg Asp Glu Ser Leu Val Tyr Arg Leu 
145                 150                 155                 160 


Gly Lys Pro Lys Ala Asn Val Glu Glu Arg Glu Phe Glu Leu Leu Tyr 
                165                 170                 175     


Asp Asp Val Gly Tyr Tyr Ile Ser Glu Ile Ile Gly Ser Gly Asp Ile 
            180                 185                 190         


Cys Asp Val Thr Gly Ala Glu Arg Met Val Glu Ile Gln Tyr Val Cys 
        195                 200                 205             


Gly Gly Ser Asn Ser Gly Pro Ser Thr Ile Gln Trp Val Arg Glu Thr 
    210                 215                 220                 


Lys Ile Cys Val Tyr Glu Ala Gln Val Thr Ile Pro Glu Leu Cys Asn 
225                 230                 235                 240 


Leu Glu Leu Leu Ala Lys Asn Glu Asp Gln Lys Asn Ala Ser Pro Ile 
                245                 250                 255     


Leu Cys Arg Met Pro Ala Lys Ser Lys Ile Gly Ser Asn Ser Ile Asp 
            260                 265                 270         


Leu Ile Thr Lys Tyr Glu Pro Ile Phe Leu Gly Ser Gly Ile Tyr Phe 
        275                 280                 285             


Leu Arg Pro Phe Asn Thr Asp Glu Arg Asp Lys Leu Met Val Thr Asp 
    290                 295                 300                 


Asn Ala Met Ser Asn Trp Asp Glu Ile Thr Glu Thr Tyr Tyr Gln Lys 
305                 310                 315                 320 


Phe Gly Asn Ala Ile Asn Lys Met Leu Ser Leu Arg Leu Val Ser Leu 
                325                 330                 335     


Pro Asn Gly His Ile Leu Gln Pro Gly Asp Ser Cys Val Trp Leu Ala 
            340                 345                 350         


Glu Val Val Asp Met Lys Asp Arg Phe Gln Thr Thr Leu Ser Leu Asn 
        355                 360                 365             


Ile Leu Asn Ser Gln Arg Ala Glu Ile Phe Phe Asn Lys Thr Phe Thr 
    370                 375                 380                 


Phe Asn Glu Asp Asn Gly Asn Phe Leu Ser Tyr Lys Ile Gly Asp His 
385                 390                 395                 400 


Gly Glu Ser Thr Glu Leu Gly Gln Ile Thr His Ser Asn Lys Ala Asp 
                405                 410                 415     


Ile Asn Thr Ala Glu Ile Arg Ser Asp Glu Tyr Leu Ile Asn Thr Asp 
            420                 425                 430         


Asn Glu Leu Phe Leu Arg Ile Ser Lys Glu Ile Ala Glu Val Lys Glu 
        435                 440                 445             


Leu Leu Asn Glu Ile Val Ser Pro His Glu Met Glu Val Ile Phe Glu 
    450                 455                 460                 


Asn Met Arg Asn Gln Pro Asn Asn Asp Phe Glu Leu Ala Leu Met Asn 
465                 470                 475                 480 


Lys Leu Lys Ser Ser Leu Asn Asp Asp Asn Lys Val Glu Gln Ile Asn 
                485                 490                 495     


Asn Ala Arg Met Asp Asp Asp Glu Ser Thr Ser His Thr Thr Arg Asp 
            500                 505                 510         


Ile Gly Glu Ala Gly Ser Gln Thr Thr Gly Asn Thr Glu Ser Glu Val 
        515                 520                 525             


Thr Asn Val Ala Ala Gly Val Phe Ile Glu His Asp Glu Leu 
    530                 535                 540         


<210>  41
<211>  1629
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  41
atgcaagcta aaattatata tgctctgagc gcaatttctg cgttgattcc gttaggatca       60

tcactattag cacctataga agaccccata gtatcgaata agtacctcat atcttacatc      120

gatgaggacg actggagtga taggatatta caaaatcagt ctgtcatgaa ctcgggatat      180

atagtgaata tgggcgacga ccttgaatgc tttattcaaa atgcaagcac tcaattgaat      240

gatgtattgg aagactcaaa tgagcatagc aatagtgaaa agacagcatt attaactaaa      300

accctgaatc aaggtgttaa gacaattttc gataaattaa atgaacggtg catcttctac      360

caagccggat tttggattta cgagtactgt cctggcatag aatttgttca gttccatggt      420

agagtaaata caaaaactgg tgaaatagta aatcgagatg aatctttggt ctaccgcctg      480

ggaaaaccaa aagcaaatgt agaagagaga gaatttgaac tactttatga cgatgtagga      540

tattacatca gcgaaattat agggtcaggt gatatttgcg atgtgacggg ggctgaaaga      600

atggttgaaa tacaatatgt ctgtggcggc tcaaactctg gaccatcgac tattcaatgg      660

gtgagagaaa caaaaatttg tgtttatgaa gcccaagtta ccatacctga attgtgcaat      720

ttagaattac tagccaaaaa tgaagaccaa aagaacgcct cacctatact ttgcaggatg      780

cccgcaaaat caaaaattgg tagtaactct attgatttaa tcaccaaata tgaaccgatt      840

tttttaggtt ctggaatata ctttctaagg ccctttaaca ccgacgaaag agacaaatta      900

atggttactg acaatgccat gtcaaattgg gatgagatta cggaaacata ttaccagaaa      960

tttggaaatg ccataaacaa aatgcttagt ttgagattag tatcgttacc taatggacat     1020

attctccagc ctggtgactc atgtgtttgg ttggcggaag tggttgatat gaaagatcgg     1080

tttcaaacca ctttatcgtt gaacatactt aattcacaga gagcagagat atttttcaac     1140

aagacgttta catttaatga agataatgga aacttcctat catacaaaat tggggatcat     1200

ggcgagtcaa ctgaacttgg tcaaataacc cactcaaaca aagcagatat aaataccgca     1260

gaaattcggt cagatgaata cttaattaac actgataatg agctattctt gaggatttct     1320

aaggagatag cagaagtgaa agaattatta aacgaaatcg taagtccaca tgaaatggaa     1380

gtaatatttg aaaacatgag aaatcaaccg aataatgatt ttgaactggc gttgatgaac     1440

aagttgaaat cctcattaaa tgatgataac aaagttgagc agataaacaa cgcaaggatg     1500

gatgatgatg aaagcactag tcatacaacc agagacatcg gggaagctgg atcacaaacg     1560

acagggaata ctgaatcgga ggtaacaaac gtagcagctg gtgttttcat cgaacatgat     1620

gagctttaa                                                             1629


<210>  42
<211>  360
<212>  PRT
<213>  Pichia pastoris

<400>  42

Met Ile Lys Val Leu Leu Phe Leu Leu Ser Leu Ser Ser Leu Val Lys 
1               5                   10                  15      


Ala Leu Asp Asp Ser Ile Asp Lys Asn Ser Val Tyr Thr Ile Asn Tyr 
            20                  25                  30          


Leu Asn His Ala Ile Ser Pro Thr Ser Glu Lys Ile Val Thr Leu Arg 
        35                  40                  45              


Ser Thr Asp Asp Gln Tyr Phe Glu Cys Leu Phe Asn Asp Glu Ile Asp 
    50                  55                  60                  


Thr Asp Gln Lys Leu His Gln Lys Gln Ile Leu Lys Thr Leu Pro Ala 
65                  70                  75                  80  


Gln Tyr Asn Leu Ser Glu Ile Pro Glu Leu Gln Thr Glu Ile Asn Ser 
                85                  90                  95      


Ala Phe Asn Ile Leu Glu Asn Tyr Asn Leu Asn Asp Ala Gln Pro Thr 
            100                 105                 110         


Lys Asp Arg Tyr Trp Thr Tyr Gln Ile Ile Asn Gly Lys Leu Tyr Gln 
        115                 120                 125             


Tyr Asn Gly Asn Leu Arg Ile Val Leu Ala Asn Ile Pro Lys Asn Leu 
    130                 135                 140                 


Thr Arg Glu Asp Ile Val Leu Glu Lys Asn Met His Gln Ser Val Phe 
145                 150                 155                 160 


Leu Ser Leu Ser Leu Gln Asn Gly Ala Ile Cys Asp Leu Thr Phe Thr 
                165                 170                 175     


Pro Arg Lys Thr Asn Ile Arg Phe Gln Tyr Val Asn Lys Leu Asn Thr 
            180                 185                 190         


Leu Gly Ile Val Ser Ala Asp Glu Ile Gln Thr Cys Glu Tyr Glu Ile 
        195                 200                 205             


Leu Ile Asn Val Pro Lys Phe Lys Asp Thr Ile Phe Gln Tyr Gly Phe 
    210                 215                 220                 


Leu Glu Pro Leu Lys Lys Ile Asp Cys Tyr Ser Ser Asp Ser Ser Met 
225                 230                 235                 240 


Ile Asn Leu Ala Asp Tyr Gln Ile Ser Val Leu Ser His Lys Trp Phe 
                245                 250                 255     


Leu Gly Ala Lys Asp Phe Arg Leu Ile Leu Ile Thr Asp Val Ser Asn 
            260                 265                 270         


Pro Pro Val Ile Ser Ile Glu Glu Leu Asn Leu Ile Phe Gln Thr Phe 
        275                 280                 285             


Pro Lys Tyr Gly Pro Pro Glu Leu Gly Ile Thr Gly Glu Ile Ser Pro 
    290                 295                 300                 


His Asp Thr Phe Ile Phe Arg Ile Pro Val Tyr Ser Tyr Asn Arg Thr 
305                 310                 315                 320 


Lys Phe Gly Asp Val Leu Val Glu Gln Asn Ile Arg Gly Glu Lys Arg 
                325                 330                 335     


Phe Leu Phe Thr Glu Asp Arg Ile Pro His Asp Thr Pro Asn Phe Arg 
            340                 345                 350         


Val Tyr Asn Gly Val Asn Val Asn 
        355                 360 


<210>  43
<211>  1140
<212>  DNA
<213>  Pichia pastoris

<400>  43
atgataaagg tcctgctatt cctgctctcc ctatcaagtc ttgtgaaagc tttggatgat       60

tccattgata agaattctgt ggtaagtctt ttaatttttg ttttcacaag atcatgccgt      120

gctaactggg tactatagta taccataaac tacttaaatc atgccatctc acccacctca      180

gaaaaaatag tgacattaag atcaacggac gatcaatatt ttgagtgttt gtttaatgat      240

gaaattgata ctgaccagaa actacatcaa aagcagattc tgaaaactct tccagctcaa      300

tacaacttga gtgaaatacc agaacttcaa actgaaataa actctgcatt caatatactt      360

gaaaactata acctcaacga tgctcagcca accaaggaca gatattggac atatcaaata      420

ataaatggaa aattgtacca atataacggg aacttgcgaa ttgtcctggc taatataccc      480

aagaatctga cgagggaaga catagttctg gagaagaata tgcaccaatc ggtgttttta      540

tcactcagct tacaaaacgg tgccatttgt gatttgactt tcactcctag aaagacaaat      600

atacggtttc aatacgttaa caagctcaac actctaggaa ttgtctccgc cgatgaaata      660

cagacctgcg aatatgaaat tcttatcaat gttcctaagt tcaaagatac catttttcag      720

tacggatttt tggagccttt gaagaagatt gattgctact cgagtgatag ctcaatgata      780

aatttggcag actaccaaat atctgtcctt tcccataaat ggttcttagg ggccaaagat      840

ttcaggttga ttttgatcac tgatgtgtct aaccctcccg tgatatcaat agaagaactg      900

aatctcatat ttcaaacatt tcctaaatac ggtcccccag agctcgggat cactggtgag      960

atttcacccc atgacacttt tatcttcaga attcctgtgt acagctacaa taggacaaaa     1020

ttcggtgacg tactggttga gcagaatatc aggggagaga aaaggttcct attcactgaa     1080

gacagaatac ctcatgacac tccaaacttt agagtgtata acggagttaa tgtgaattaa     1140


<210>  44
<211>  520
<212>  PRT
<213>  Aspergillus fumigates

<400>  44

Met Ile Arg Arg Ile Arg Thr Leu Thr Pro Leu Leu Val Leu Ala Cys 
1               5                   10                  15      


Ala Gly Ser Gly Ala Trp Ala Ser Lys Lys Ala Phe Asn Ile Gln Asp 
            20                  25                  30          


Asp Leu Leu Ala Tyr Pro Gln Phe Gln Val Phe Phe Pro Asp Glu Tyr 
        35                  40                  45              


Ile Leu Asp Ala Arg Ala Arg Glu Leu Leu Gln Asn Gln Gln Glu Ser 
    50                  55                  60                  


Ser Ser Ala Ser Ala Asp Lys Thr Phe Ser Glu Gly Asn Asp Ala Gln 
65                  70                  75                  80  


Val Tyr Leu Gly Ser Arg Lys Asp Gln Ser Glu Asp Val Asn Lys Glu 
                85                  90                  95      


Thr Ile Glu Gly Ser Gly Phe Thr Tyr Glu Glu Met Leu Leu Glu Gly 
            100                 105                 110         


Gln Arg Tyr Leu Cys Ser Ile Pro Gln Val Asp Asn Gly Asn Arg Asp 
        115                 120                 125             


Gln Thr Asn Gly Ala Glu Ser Thr Ser Lys Glu Asp Glu Gln Arg Glu 
    130                 135                 140                 


Ile Ala Arg Ala Thr Asp Arg Gly Leu Glu Leu Leu Arg Glu Met Glu 
145                 150                 155                 160 


Gly Lys Cys Met Tyr Tyr Ile Ser Gly Trp Trp Ser Tyr Ser Phe Cys 
                165                 170                 175     


Tyr Lys Lys Gln Ile Lys Gln Phe His Ala Leu Pro Ser Gly Pro Gly 
            180                 185                 190         


Val Pro Asn Tyr Pro Pro Ile Glu Asp Ser Thr Thr His Ser Phe Val 
        195                 200                 205             


Leu Gly Arg Phe Pro Asn Ser Gly Asp Asp Glu Asp Leu Glu Gly Asp 
    210                 215                 220                 


Ala Glu His Lys Lys Thr Thr Thr Asp Val Ala Glu Leu Gln Thr Lys 
225                 230                 235                 240 


Gly Gly Ser Arg Tyr Leu Val Gln Arg Leu Gly Gly Gly Thr Lys Cys 
                245                 250                 255     


Asp Leu Thr Gly Lys Asp Arg Lys Ile Glu Val Gln Phe His Cys His 
            260                 265                 270         


Pro Gln Ser Thr Asp Arg Ile Gly Trp Ile Lys Glu Leu Thr Thr Cys 
        275                 280                 285             


Ser Tyr Leu Met Val Ile Tyr Thr Pro Arg Leu Cys Asn Asp Val Ala 
    290                 295                 300                 


Phe Leu Pro Pro Gln Gln Asp Glu Ala His Ala Ile Glu Cys Arg Glu 
305                 310                 315                 320 


Ile Leu Ser Glu Glu Glu Val Ser Asp Trp Glu Ala Asn Arg Glu Tyr 
                325                 330                 335     


His Leu Ala Gln Gln Leu Val Glu Ser Ala Ile Thr Pro Glu Phe Pro 
            340                 345                 350         


Val Val Gly Asp Ile Glu Val Gly Ala His Lys Trp Val Gly Ser Glu 
        355                 360                 365             


Gly Lys Gln Ile Glu Lys Gly Arg Val Ala Ser Ile Gly Glu Glu Lys 
    370                 375                 380                 


Ile Glu Val Val Ala Lys Arg Gln Asn Gly Glu Ile Thr Arg Leu Ser 
385                 390                 395                 400 


Lys Glu Glu Leu Lys Lys Tyr Gly Leu Asp Pro Glu Lys Ile Glu Thr 
                405                 410                 415     


Leu Lys Ser Arg Leu Glu Glu Leu Ala Lys Gly Lys Asp Trp Thr Leu 
            420                 425                 430         


Glu Ile Val Glu Ser Asn Gly Glu Arg Gly Leu Val Gly Thr Val Asp 
        435                 440                 445             


Ser Asn Asp Asp Glu Lys Glu Asp His Ala Ala Gln Gly Ser Ile Ser 
    450                 455                 460                 


Gln Pro Ala Gln Gly Thr Thr Ala Asp Lys Gly Glu Ser Asn Ala Glu 
465                 470                 475                 480 


Thr Gly Glu Glu Lys Lys Lys Ala Asp Glu Lys Ile Asp His Tyr Glu 
                485                 490                 495     


Pro Glu Lys Ser Gly Pro Thr Thr Asp Asp Ala Asp Asp Gly Ser Glu 
            500                 505                 510         


Glu Ile Phe Phe Lys Asp Glu Leu 
        515                 520 


<210>  45
<211>  1563
<212>  DNA
<213>  Aspergillus fumigates

<400>  45
atgattcgac gtatacggac tcttacccca ttgctggtgc tggcttgtgc tggttccggc       60

gcatgggcca gcaagaaggc gttcaacata caagatgatc tacttgcata tcctcaattt      120

caagtcttct tccctgatga atacattctt gatgcgcgag caagggagtt attacagaat      180

caacaagaga gctcttcggc ttccgctgat aagacattct ccgaaggcaa tgatgcgcaa      240

gtatatctgg gaagccgaaa agatcaatct gaagacgtca ataaagagac gatagaagga      300

tctgggttca catacgagga gatgctcctt gagggacaga gatatctctg ttccattccg      360

caagtcgaca acggaaacag ggaccagacg aacggagcgg aaagcaccag taaagaggat      420

gaacagcgag aaattgcacg cgcgacggac cgtggcctgg aacttctgcg cgagatggaa      480

ggcaaatgca tgtactacat atccggatgg tggtcatact cattctgcta caagaagcaa      540

atcaagcagt ttcatgcact accgtccggt ccaggcgtgc ccaactaccc gccgatagaa      600

gactctacga cccattcttt cgtgctgggc aggtttccca acagcggcga cgacgaggat      660

ttggaggggg atgcggagca caaaaagaca actacagatg tcgccgagct ccagactaaa      720

ggcgggtcgc ggtacttagt gcagcggctg gggggcggaa ccaagtgcga cttgacaggc      780

aaagaccgga agatcgaagt gcagttccac tgccatccgc aatctacaga tcggatcggt      840

tggatcaagg aacttactac ttgctcatat ctcatggtga tctacactcc gcgcttgtgc      900

aatgatgtcg catttctgcc gcctcagcag gacgaggctc acgcgatcga atgccgcgag      960

attctctccg aggaagaggt ttccgactgg gaagcaaacc gggaatatca tttggctcag     1020

cagctcgtcg aatcagcgat tacacccgag tttcctgttg tcggggatat cgaggtcggg     1080

gcgcacaagt gggtgggatc ggaaggcaag cagatcgaga agggtcgagt ggcatccatt     1140

ggagaagaga agatcgaggt agttgccaag cgccaaaatg gagagatcac aaggttgtcc     1200

aaggaggagt tgaagaaata cggtcttgat cctgagaaga ttgagacgct gaaaagccgc     1260

ctcgaggagc ttgccaaggg taaggactgg acactggaga ttgtcgagtc taacggcgag     1320

cgtggcttag tcggaactgt cgactccaac gacgatgaga aagaggatca cgccgcacag     1380

ggctctatat cgcagccggc acagggaact acagctgaca agggggaatc caatgcagag     1440

acaggagagg aaaagaagaa ggcagacgag aagatagacc attacgagcc agaaaaatca     1500

gggccgacca ctgatgatgc cgacgacggc agcgaggaaa tcttcttcaa ggatgagctc     1560

tag                                                                   1563


<210>  46
<211>  305
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  46

Met Phe Pro His Leu Ile Leu Pro Ala Ile Gly Ser Ser Lys Val Arg 
1               5                   10                  15      


Thr Met Val Leu Pro Phe Ala Phe Val Gly Phe Phe Ile Phe Pro Ile 
            20                  25                  30          


Cys Leu Ala Ser Leu Leu Asp Trp Asn Asp Ala Tyr Glu Tyr Pro Lys 
        35                  40                  45              


Tyr Ser Phe Glu Trp Ser Asn Val Ser Ile Leu Glu Gly Asp Ile Asp 
    50                  55                  60                  


Ser Ile Lys Glu Lys Thr Glu Lys Thr Lys Leu Ser Ser Leu Phe Tyr 
65                  70                  75                  80  


Ala Gly Lys His Glu Tyr Phe Cys Val Tyr Pro Asn Ala Ser Leu Ile 
                85                  90                  95      


Lys Gln Asn Ser Thr Thr Glu Pro Ser Tyr Asp Leu Gln Glu Leu Arg 
            100                 105                 110         


Ile Gln Gly Thr Glu Lys Ile Asn Glu Leu Ala Asn Val Phe Leu Ile 
        115                 120                 125             


Glu Asn Arg Gly Tyr Trp Thr Tyr Asp Tyr Val Tyr Gly Gln His Val 
    130                 135                 140                 


Arg Gln Tyr His Leu Glu Pro Gln Gln Gly Ser Asp Lys Val Leu Ala 
145                 150                 155                 160 


Asn Pro Met Tyr Ile Leu Gly Thr Ala Pro Asn Thr Gln Thr Lys Lys 
                165                 170                 175     


Asn Leu Glu Glu Asn Trp Ala Ile Gly Phe Val Glu Gly Lys Ala Tyr 
            180                 185                 190         


Leu Gln Thr Thr Phe Arg Asn Gly Thr Met Cys Asp Ile Thr Lys Arg 
        195                 200                 205             


Pro Arg His Val Ile Leu Ser Tyr Glu Cys Ser Thr Asn Ser Asp Thr 
    210                 215                 220                 


Pro Glu Ile Thr Gln Tyr Gln Glu Val Ser Ser Cys Ala Tyr Ser Met 
225                 230                 235                 240 


Thr Ile His Val Pro Gly Leu Cys Ser Leu Pro Ala Phe Lys Ile Gln 
                245                 250                 255     


Glu Asp Ile Pro Ser Glu Lys Ile Val Cys Tyr Asn Val Ile Lys Glu 
            260                 265                 270         


Lys Ser Asn Glu Val Asp His Lys Asp Ser Gln His Val Val Asp Glu 
        275                 280                 285             


Val Ala Gln Thr Ser Pro Pro Glu Val Lys Glu Val Glu Thr Gln Ser 
    290                 295                 300                 


Ser 
305 


<210>  47
<211>  918
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  47
atgtttccac atttgattct acctgcaatc ggctcatcta aagttaggac tatggtgcta       60

ccatttgctt ttgtggggtt ttttattttt ccaatatgtt tagcttcttt gttagactgg      120

aatgatgcat atgaatatcc taaatattcg tttgaatgga gtaatgtgtc aatattagag      180

ggcgacattg actcaattaa agaaaaaact gaaaaaacta aattatcgtc attattctat      240

gctggaaagc atgaatattt ttgtgtatat cccaatgcgt ctcttataaa acaaaatagc      300

acaaccgaac caagctatga tttacaagaa ttgcggatac aagggactga aaaaatcaat      360

gagcttgcta atgtattttt aatcgagaat cgtggttatt ggacttatga ctatgtctac      420

ggtcaacacg tgcgtcaata tcatttggag ccgcagcaag gttctgacaa agtccttgct      480

aaccctatgt atatacttgg tacggcacct aacactcaaa ctaaaaagaa tttggaagaa      540

aattgggcta ttggatttgt tgaaggtaaa gcatatttgc aaacaacttt ccgaaatggg      600

actatgtgcg acattactaa gagaccaaga cacgtaattc taagttatga atgcagtaca      660

aattcggata ctcctgaaat tactcaatat caagaagttt caagctgtgc atattcaatg      720

actattcacg ttcccggttt atgctcatta cctgctttca aaattcaaga ggacataccc      780

tctgaaaaaa ttgtgtgcta taatgtaatt aaagaaaaat caaacgaagt cgaccataag      840

gattcccagc acgttgttga tgaagttgct caaacatctc cgcctgaggt gaaggaggta      900

gagacgcaat caagttag                                                    918


<210>  48
<211>  2004
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Pichia pastoris ATT1 5' region in pGLY5933

<400>  48
ggccgggact acatgaggcc gattcttcaa gccagggaaa ttaattgctt gaaccggaaa       60

atcattaagg caggcaacga aaaatccaac tccttggttg aattgactca aaagtttatc      120

ttacggagaa aagctaaaga catcaatacg aatttccttc cgccaaaaac tgaactgata      180

ctgatggttc caatgactga attacaacag gagctataca aggatataat tgaaactaac      240

caagccaagc ttggcttgat caacgacaga aacttttttc ttcaaaaaat tttgattctt      300

cgtaaaatat gcaattcacc ctccctgctg aaagacgaac ctgattttgc cagatacaat      360

ctcggcaata gattcaatag cggtaagatc aagctaacag tactgctttt acgaaagctg      420

tttgaaacca ccaatgagaa gtgtgtgatt gtttcaaact tcactaaaac tttggacgta      480

cttcagctaa tcatagagca caacaattgg aaataccacc gactagatgg ttcgagtaaa      540

ggacgggaca aaatcgtacg agattttaac gagtcgcctc aaaaagatcg attcatcatg      600

ttgctttctt ccaaggcagg gggagtgggg ctcaacttaa ttggagcctc acgcttaatt      660

ctttttgata acgactggaa tcccagtgtt gacattcaag caatggctag agtgcatcga      720

gacgggcaga aaaggcacac ctttatctat cgtttgtata cgaaaggcac aattgacgaa      780

aagatcctac aaaggcaatt gatgaaacaa aatctgagcg acaaattcct ggatgataat      840

gatagcagca aggatgatgt gtttaacgac tacgatctca aagatttgtt tactgtagat      900

cttgacacga attgtagtac acacgatttg atggaatgtt tatgtaatgg gcggctgaga      960

gatccgactc ccgtcttgga agcagaagaa tgcaagacaa aaccgttgga ggccgttgac     1020

gacacggatg atggttggat gtcagctctg gatttcaaac agttatcaca aaaagaggag     1080

acaggtgctg tgtcaacaat gcgtcaatgt ctgctcggat atcaacacat tgatccaaag     1140

attttggaac caacagaacc tgtaggggac gatttggtat tggcaaacat cctcgcggag     1200

tcctcaggct tggctaaatc tgcattgtca tctgaaaaga aacccaagaa accagtggtg     1260

aactttatct ttgtgtcagg ccaagactaa gctggaagaa cggaacttta atcgaaggaa     1320

aaattaaatg tcaaagtggg tcgatcagga gataatccat gcttcacgtg atttttctta     1380

ataaacgccg gaaaaacttt cttttttgtg accaaaatta tccgatctga aaaaaaatta     1440

cgcatgcgtg aagtaggatg agagacttac tgttgaactt tgtgagacga ggggaaaagg     1500

aatatcctga tcgtaaacaa aaaagttttc cagcccaatc gggaacatct gcgaagtgtt     1560

ggaattcaac ccctctttcg aaaatgttcc attttaccca aaattattgt tattaaataa     1620

tacatgtgtt actagcaaag tctgcgcttt ccatgtctca gattcggcag ataacaaagt     1680

tgacacgttc ttgcgagata cgcatgaatc ttttggctgc tttttgtgaa agagaaatgg     1740

tgccatatat tgcagacgcc cctgaaagat tagtgtgcgg ctgagtcttt tttttttctc     1800

aaccagcttt ttctttttat tgggtaccat cgcgcacgca ggactcatgc tccattagac     1860

ttctgaacca cctgacttaa tattcatgga cggacgcttt tatccttaaa ttgttcatcc     1920

attcctcaat ttttccgttt gccctccctg tactattaaa ttacaaaagc tgatcttttt     1980

caagtgtttc tctttgaatc gctc                                            2004


<210>  49
<211>  1854
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Pichia pastoris ATT1 3' region in pGLY5933

<400>  49
ggaccctgaa gacgaagaca tgtctgcctt agagtttacc gcagttcgat tccccaactt       60

ttcagctacg acaacagccc cgcctcctac tccagtcaat tgcaacagtc ctgaaaacat      120

caagacctcc actgtggacg attttttgaa agctactcaa gatccaaata acaaagagat      180

actcaacgac atttacagtt tgatttttga tgactccatg gatcctatga gcttcggaag      240

tatggaacca agaaacgatt tggaagttcc ggacactata atggattaat ttgcagcggg      300

cctgtttgta tagtctttga ttgtgtataa tagaattact acgcgtatat cccgatctgg      360

aagtaacatg gaagtttccc attttcgcgc agtctcctac tcgtatcctc cccacccctt      420

accgatgacg caaaaggtca ctagataagc atagcatagt ttcatccctt gctctttcct      480

tgtaccaaca gatcatggct gggaatctca aggatattct atccttgtcg aggaagacag      540

caaggaatct gaagcaggct ctggatgagc ttgcggagca ggtgatcaac caccaacgga      600

gacgaccagc tctggtccga gttcctatca acaacaacct taggcgcaag agccagcagt      660

cctttttgaa tcgcaggtca ttccatcttt ggaccagcaa gtacaaccca tacttttgga      720

ggggaggcag aagcaacgtt ctggaccagc ttaaccgtga agctttaagg tacagatcgt      780

cttttgcgaa acccggattt tatccaagtg ggctgtatca gtcaactttc cctcaaagag      840

gtagtaggat gttttccacc tgcgcctact catgtcagca ggaggcagtc aaaaacttga      900

cttccgctgt tcgtgctttg ttacaaagtg gtgctaattt cggcagtcaa atgaaacaaa      960

tgaaacactg ttcgcaaaag aagaagcact tctctaaatt ttctaagagg cttacttctt     1020

ccactgccgc tgggtctggc aagaatgctg aacaagctcc ttctggtttg gccgaaggat     1080

ccgctgttgt ttttagcctt gaacgtcaaa gtcacaatac tgagttggaa ggaatcttgg     1140

atcaagaaac ttcttccatt ctcgaggaag aaatggttca acatgagcgt cacctggcta     1200

ttattagaga agaaatccag agaattagtg agaatctagg atcattacca ttaatcatgt     1260

ctggtcacaa gattgaggta tttttcccca attgtgacac tgttaaatgt gagcaactga     1320

tgagagattt ggctattacg aaaggggttg tgaggcgtca tgattctact gctgagcatt     1380

caagctccag gtcatttgtt ccagaagatt gcttgtattc ctcagggtca agttcaccga     1440

atcctttatc ctcaacttct tcgaaatcat ttgatagagt ctcattggac tacatttcct     1500

ctcggtctac atctgatcaa accactggtt ctgagtacac atctctgtct caacaatatc     1560

acctggttag caattacaac cctgtactat cctcagcccc gggttcttcg agggtcttgg     1620

agctgaatac tcccgagtcc actatggaag gcagtacaga tctggagtat ttaacgcgag     1680

acgatgtgtt gctgttaaat gtctaatcta gacctatcct tcattctata tagcttagtt     1740

gagttttacg taagccctag tttttgttaa ttcttatcga tttatggtta gtgtaccact     1800

caactcacga tgatatatcc caggagctgt ttgtgcatta taactaccaa tcct           1854


<210>  50
<211>  1392
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA encodes Homo sapiens endomannosidase (codon-optimized for 
       expression in Pichia pastoris)

<400>  50
atggctaagt tcagaagaag aacctgtatt atccttgctt tgtttatttt gtttatcttt       60

tcccttatga tgggattgaa gatgttgaga cctaacaccg ccacttttgg tgcaccattc      120

ggacttgatt tgcttcctga attgcatcaa agaactatcc atttgggtaa aaacttcgat      180

ttccagaaat cagacagaat caatagtgaa acaaacacca agaatttgaa gtcagttgag      240

atcacaatga agcctagtaa agcttctgaa ttgaatcttg atgagcttcc acctttgaac      300

aactatttgc atgttttcta ctatagttgg tacggtaacc cacaattcga tggaaagtat      360

atccattgga atcacccagt cttggaacat tgggacccta gaattgctaa aaactaccca      420

cagggtagac acaatccacc tgatgacatt ggttcttcct tttatcctga attgggatct      480

tactcaagta gagatccatc cgttattgag actcacatga gacaaatgag atcagctagt      540

atcggtgttt tggccctttc ttggtatcca cctgatgtca acgacgaaaa tggagagcca      600

actgataacc ttgttcctac aattttggac aaggctcata aatacaactt gaaggtcact      660

ttccacattg aaccttattc caatagagat gaccagaaca tgtacaagaa cgttaagtac      720

atcatcgata agtacggtaa ccatccagca ttctacagat acaagactaa gacaggaaat      780

gctttgccta tgttctacgt ctatgactct tacattacta agccagagaa atgggctaac      840

ttgcttacta catctggttc cagatcaatt agaaattctc cttacgatgg actttttatc      900

gccttgcttg ttgaagagaa gcataagtac gatatcttgc aatccggttt cgacggaatc      960

tacacttatt ttgccacaaa cggtttcacc tacggatctt cccaccagaa ttgggcatct     1020

ttgaagttgt tttgtgataa gtacaatttg attttcatcc catcagtcgg tcctggatat     1080

attgacactt ctatcagacc atggaacacc caaaacacta gaaacagaat taatggtaaa     1140

tactacgaaa tcggactttc cgctgccttg caaaccagac cttccttgat ttcaatcact     1200

tcttttaacg aatggcatga gggtactcag attgaaaagg ctgttccaaa aagaacatca     1260

aataccgtct acttggatta tagaccacac aagcctggat tgtaccttga gttgacaaga     1320

aaatggtctg aaaagtattc caaagagaga gcaacctacg ctcttgacag acaattgcca     1380

gtttcttaat ga                                                         1392


<210>  51
<211>  1416
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mouse mannosidase  IB catalytic domain

<400>  51
gatccagaag acatggagat caagaagaaa agagacaaaa ttaaagagat gatgaaacat       60

gcctgggata attacagaac atacggatgg ggacataatg aactaaggcc tattgcaagg      120

aaaggccatt ccactaacat attcggaagc tcacagatgg gtgccaccat agtggatgct      180

ttggataccc tttatatcat ggggcttcat gatgaattca tggatgggca aagatggatt      240

gaagaaaacc ttgatttcag tgtgaattca gaagtgtctg tctttgaagt taacattcgc      300

tttattggag ggctcctcgc tgcatattac ctgtcaggag aggaaatatt caagactaaa      360

gcagtgcagt tggctgagaa actccttcct gcctttaaca cacctactgg gattccctgg      420

gcaatggtga acctgaaaag tggagtaggt cgaaactggg gctgggcgtc tgcaggcagc      480

agcatcctgg ctgagttcgg caccctgcac atggagtttg tgcacctcag ctacttgacc      540

ggtgacttga cttactataa taaggtcatg cacattcgga aactactgca gaaaatggaa      600

cgcccaaatg gtctttatcc aaattattta aacccaagaa cagggcgctg gggtcagtat      660

cacacatcag ttggtggtct gggagatagt ttttatgaat acttactgaa agcatggctg      720

atgtcagata aaacagacca cgaggcaaga aggatgtatg acgatgctgt tgaggctata      780

gaaaaacatc ttattaagaa gtcccgagga ggtctggttt ttattggaga atggaagaat      840

ggacacttgg aaaggaagat ggggcacttg gcctgctttg ctgggggaat gtttgccctt      900

ggagcagatg gttccagaaa ggataaagct ggccactact tagaactagg ggcagaaatt      960

gcacgaacat gtcatgagtc atatgacaga actgcattga aactaggtcc ggagtcattc     1020

aagtttgatg gtgcagtgga agccgtggct gtgcggcagg ctgaaaagta ttacatcctt     1080

cgtccagaag taattgaaac ctattggtat ctatggcgat ttacccacga cccaagatac     1140

aggcagtggg gctgggaagc agcactggct attgagaagt cgtgccgggt cagcggtggg     1200

ttttctggtg tcaaggatgt atacgccccg acccctgtgc atgacgacgt gcagcagagc     1260

ttttttcttg ctgaaacatt aaaatacttg tacctgctgt tctctggcga tgaccttcta     1320

cctttagacc actgggtgtt taacacagag gcgcaccctc tgccggtgtt gcgcttagcc     1380

aacagcactc tttcaggtaa tcctgctgtc cgatga                               1416


<210>  52
<211>  426
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SEC12 leader 9

<400>  52
atgaacacta tccacataat aaaattaccg cttaactacg ccaactacac ctcaatgaaa       60

caaaaaatct ctaaattttt caccaacttc atccttattg tgctgctttc ttacatttta      120

cagttctcct ataagcacaa tttgcattcc atgcttttca attacgcgaa ggacaatttt      180

ctaacgaaaa gagacaccat ctcttcgccc tacgtagttg atgaagactt acatcaaaca      240

actttgtttg gcaaccacgg tacaaaaaca tctgtaccta gcgtagattc cataaaagtg      300

catggcgtgc atgagacgag ttctgtgaat ggaactgaag tcttatgtac tgaaagtaac      360

attattaata ctggaggggc agagtttgag atcaccaacg caacttttcg agaaatagat      420

gatgct                                                                 426


<210>  53
<211>  502
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PpSTT3 promoter

<400>  53
accagtcttg aagattcaga cgtagacatg gataaatttg ttgacgctat ggatatttca       60

ccgttgccag atgccgcaga ttcgtcattt tctacggtta aagcttccag acagagttca      120

ctaaccacaa gaaaactaat tccgtccaaa caaagtaaga gccttctaag ctctttgaag      180

aacgctgaag ctcaaccaga tgaaacagaa atagttccac ccttaggtgc accctcacga      240

atgaatttgg tagaacctaa tatggtgctt gaagataata ataaatagat caatcaactc      300

accgaacaaa tgattatata attgggctct cctttcctgc tagcccttgc acttcccttc      360

cctagtaaat acatccgaga gcatccttcg cgaatacctt ccaacacata aacagtacac      420

tactccgccg aaaaagacac gttggagcga ctagcttaaa atactctcca ccgccaaatc      480

ctccctcaac ggatctccaa ca                                               502


<210>  54
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Insulin analogue B chain (des B30) :Asn at 1 and 31 beta-1 linked
       to a paucimannose N-glycan

<400>  54

Asn Gly Thr Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu 
1               5                   10                  15      


Ala Leu Tyr Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Asn Lys 
            20                  25                  30          


<210>  55
<211>  85
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sc alpha mating factor signal sequence and pro-peptide

<400>  55

Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser 
1               5                   10                  15      


Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln 
            20                  25                  30          


Ile Pro Ala Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe 
        35                  40                  45              


Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu 
    50                  55                  60                  


Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val 
65                  70                  75                  80  


Ser Leu Glu Lys Arg 
                85  


<210>  56
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminal spacer

<400>  56

Glu Glu Ala Glu Ala Glu Ala Glu Ala Lys 
1               5                   10  


<210>  57
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B-chain

<400>  57

Asn Gly Thr Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu 
1               5                   10                  15      


Ala Leu Tyr Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Asn Lys 
            20                  25                  30          


<210>  58
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A-chain

<400>  58

Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys Asn 
            20      


<210>  59
<211>  1826
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PpADE4-5UTR+ORF

<400>  59
tacacgatca caagttgtgt atagtctttt ctttaaactg atcgtagacc agaccaccac       60

agcgtagcca aatgttattt attcattaat cgaaaaagtt ttggttcagg cgcgacaagg      120

tagtaagaaa aaaattctgc atgaattgat tcttcacttg gtacttgatt cattgaacaa      180

tataaacaca gataatgtgt gggattcttg gaattgtatt ggctgatcag tcagaagatg      240

ttgcagctga attgttagat ggagccatgt ttttgcaaca taggggacaa gatgccgcag      300

gtattgtgac ctgtgcagga ggacgttttt atcaatgcaa aggtaatgga atggccaagg      360

acgtacttac ggagcaacgt atgaaagggc tggtaggtaa tatgggaatt gcgcagctaa      420

gatatccgac tgctggttct agtgccatga gcgaagcgca gccgttttat gttaacagtc      480

catacggaat tgcactttct cataatggta atcttgtgaa tggacgtaat ctccgccaga      540

aattagatga tgttcttcat cgccatataa atacagatag tgatagcgag ttactgttga      600

acatttttgc tgctgagttg gctcagtacg acaagaaaag agttaactca gaagacattt      660

tcaaggccct cgttggtgtc tacagagaat gtcgtggagc ttatgcttgt gtcagtatgt      720

tggccggcta tggtattatt ggatttcgtg atcctcatgg tatcagacct ttagtcgtcg      780

gagaacgtgt gagagtgtcc caaactcccg gtgacactca cttgcaatgc gattatatgc      840

ttgcctctga gagtgtagtt ttaaaggctc atggatttca caactttagg gatattttac      900

caggtgaagc tgttattatc acaaagagag ggcctccgga gttttgtcaa attgttcctg      960

cgaaagccta cactccggat atttttgaat acgtttattt tgctagacct gattcgatta     1020

tggatggaat atctgtctac cgaagccgtt tggcaatggg gcgcaaacta gcccagaaaa     1080

tcacctctcg ttttaccagt cagtccttaa acgtagttag agaaattgat gtggtgatac     1140

ctgttccaga tacatctcga ccttcagctc tggaatgtgc cgtgacgctt ggcataccat     1200

tcagagaagg ttttgtcaaa aatcgttatg tgggccgtac cttcattatg ccgaaccaga     1260

aggaaagaac ttcgtctgtg cgacgtaaat taaacgctat gtcttctgag tttgctggtc     1320

gtaacgtttt gttaattgac gactcgatcg taagaggaac cacgtccaag gaaatcgtta     1380

acatggcaag agaagctggc gctaacaaag tatactttgc atcatgctct ccagtcatac     1440

gatacaatca tatatatggc attgacctcg cagattcacg tgctttggtg ggatttggtc     1500

gatcagaaag ggaggtatct gacttgatag gtgctgacga tgtaatttac cagtcacttg     1560

atgatttgaa atcctgttgt gttcaggagc ccgaactccc atccgagtta ccctcaacta     1620

ggattgcatt cacccaacca cctccgaaga ttaatggatt tgaggtgggt gtattcaccg     1680

gagtttatgt aactggagag gaagatcatt atctcaagga gttagaacag gtaagagcta     1740

aaaatgagcg atcacgtatt aatggctgtg gtatagacgt taaagcggag actgatattt     1800

ctttgtttaa tagaggggag agttga                                          1826


<210>  60
<211>  1220
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PpADE4-3UTR

<400>  60
tagtaaagag catatcagtt gcaaatctca tacttacatc tgtccaattc gtcaatcatg       60

caactagtgt gtctaaccgc taatgtgcaa ccaaatccaa ttaatggaag aataaagtct      120

tccgtaaatt ggtttgcttc gcaaatctct cgatatatga ggtattaaag aaagtaagaa      180

tatgaaatcg taactggtaa tagatggatg tatctagaat caaccaacta ataagacaaa      240

cattgtttgc agcgctatca tgtcttttac agtaagtctt ttctgtcaag tggataaacg      300

ggtcaaaaat tataatgatg tacgtacgtt cgccttcgca ccataaacga cgaggcctaa      360

tttttactat ataataacaa aagttaagac agtaataccc tgtcgcttta catcagacaa      420

aatcatgttg ttgagtagtc agtcattgat tcatgagttc atttctaaat acttgaaatc      480

caatatgaac tacctcacaa tttaaaaagg aagataatca atcctattat tcgctggcca      540

ccgtaatgcc atattcggat cagatgaaaa cgaagcatag gttgaatata agcaatctaa      600

cttcgttcag catttgctct gaaaaataca ccaaaaaaac atgcgattta gattgtgatg      660

ctgctcttga ccctgcccta tgtttcaaac tacggatcac tttcttaaaa aagccgggct      720

gcatatttcc agataatcat gacgcatcca cctcgttaca atgtacctaa actaaaagac      780

aacgacagcc cccttggttg tgcagatcat catctcctat caaacagcac acaaaaaact      840

gggtaataag tttagaacga gttacaaaat gtcttcctcc ttttgcaatt ctaatctacg      900

cggaatctgt caccctctta ggtttattct cttacagtac ttcccctaga atcccgacaa      960

gagctaaaca aaaacttagg ccagaaagca aagttccctt agcatataat ttacctagct     1020

ttgttaggct atttcgaact tgattccgtt caatcgccca ctccacttca tcttcgacat     1080

tatcttccat caattctcct tctacagaaa cataggctga cccatctaaa gaagatcttt     1140

cagtaatgtc ttgtttcttt tgttgcagtg gtgagccatt ttgacttcgt gaaagtttct     1200

ttagaatagt tgtttccaga                                                 1220


<210>  61
<211>  1025
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PpADE8-5UTR+ORF

<400>  61
aacccaactg ctctgctgtt atcctcatgc atgatgttga gacacatgtc tttgaacagc       60

tatgccgaca agatcgaaaa ctctgtcttg aagaccattg cttctggacc agagcacaga      120

actaaggact tgaaaggaac ctcctcgact tcaaacttca ccgaacaagt tatcaagaac      180

ttgtaatagt gaacggttat gaaaatgaat gcttcatgac ttgaggctcc tttcgttaga      240

aatatagata gatgtagcag tcttttgaaa cggttgaaaa atgtattaac gatctttact      300

agtaattatg gtttgcagtt cgcacttttt tttttcagcc tttatcatcg atcacactag      360

gaaaaaaaaa tcaagctagt ctagtaacga tgacgcctaa gatattagta ctcatttctg      420

gtaatggaag caacctccag gctctcatta atgccaagga gcaaggccag ctgaaagcag      480

aaatatcttt ggtcatatcc tcaagtagta aggcatttgg catcgaaaga gccaggaaac      540

acaacattcc agtccgagtg catgagctga agtcatacta ccagggaatt cccaaagagg      600

agaaagccaa acgagccgaa aagagaaacg attttgatca agacctggtc aagatcatat      660

tgagcgagaa gcctgatctt gttgtttgtg ccggctggat gcttatacta ggtgaaaaat      720

tcttacaacc tttacaagag aagaacatct ccatcataaa cttgcatcca tccttgcctg      780

gagcctttga gggaattaat gcaatcgaaa gatcttataa tgccggtcag aatggcgaaa      840

ttactaaggg tggtatcatg atccatcggg ttattctgga ggttgataga ggacaacctc      900

tcatagtgag agaaatagat gttatcaaag gagagacgct agagtcgtgg gaggcaagaa      960

tccattcttt agaacaccaa gcaatagtgg atggaactaa caaggcattg gacgagttga     1020

aataa                                                                 1025


<210>  62
<211>  1077
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PpADE8-3UTR

<400>  62
gggtcacata taagccaatt aatttcttca atttctttta tccgttaaca gtatgttgta       60

tatctttatg cttcagtatc tacctccatt ggaaccacag tttcctcaat atcgacaaga      120

ttgtagatac tctctttcaa caccgcagta gtgcctctag caaacttgta tgacttaacc      180

ttggcttcac ggacgttagg cttcagatag tttctgtaca attgggcatc ttttccaact      240

tccatgacac aaaggtccac gttagaaccg gatcccaaat cattccagat acctgcctca      300

atagcttctg tgcacagctt cattgcctct tccttagtca agccttcttt ccagttgctc      360

tccaagacag ccatggccgc cagcgaacct gatcccaaag attgatagaa tccaatatcg      420

gtggatccat gcgcatggat agaaaacaag tgggctcctg taggatcaac accaccgacg      480

attagatagg ctccaatgtg accttggtac ttgaacagat gttgtttcag catcgtcaat      540

gctgtgacca ctcgaggttt cctttctgta gacatggcat gcaattctag atttgatcca      600

atcagttgtg taaccatctc tgtatcagca gcggtacctg ctcctgcaca ccatatagta      660

ggtgacaacc tgtgtagctt ttcacaattc ttgtcagcca cgataggacc tgacgtagct      720

ctggtatcgg cagcgatcac aacacctcct tcaaatttaa ctcccacaat ggtggttcct      780

gtggaagttg ccttcggaga gccaaatccc ttagctgaaa ggaattgatt tctttggtgg      840

ttatcgaaac tgaggcctgc catattcgtg tgatggtatg gtgaacgagt ttgtcaagtg      900

ggttgaattt gccttcagga tattacgatt cgagaagtac attctatcga tgtcggatgt      960

gagatacata cattaaaact catacgtcaa aggtatgcaa ctacggctct caagaacctt     1020

ttatatacag gacgttagtt gaccaccttg tcacatctat ggcacagttc gtgatct        1077


