                         SEQUENCE LISTING

<110>  F. Hoffmann-La Roche AG
 
<120>  Hocheffiziente Herstellung von Blutglucose-Teststreien

<130>  46199P WO

<150>  EP13156363.7
<151>  2013-02-22

<160>  2     

<170>  PatentIn version 3.5

<210>  1
<211>  261
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutated glucose dehydrogenase (E96G, E170K)


<220>
<221>  MUTAGEN
<222>  (96)..(96)
<223>  E96G

<220>
<221>  MUTAGEN
<222>  (170)..(170)
<223>  E170K

<400>  1

Met Tyr Pro Asp Leu Lys Gly Lys Val Val Ala Ile Thr Gly Ala Ala 
1               5                   10                  15      


Ser Gly Leu Gly Lys Ala Met Ala Ile Arg Phe Gly Lys Glu Gln Ala 
            20                  25                  30          


Lys Val Val Ile Asn Tyr Tyr Ser Asn Lys Gln Asp Pro Asn Glu Val 
        35                  40                  45              


Lys Glu Glu Val Ile Lys Ala Gly Gly Glu Ala Val Val Val Gln Gly 
    50                  55                  60                  


Asp Val Thr Lys Glu Glu Asp Val Lys Asn Ile Val Gln Thr Ala Ile 
65                  70                  75                  80  


Lys Glu Phe Gly Thr Leu Asp Ile Met Ile Asn Asn Ala Gly Leu Gly 
                85                  90                  95      


Asn Pro Val Pro Ser His Glu Met Pro Leu Lys Asp Trp Asp Lys Val 
            100                 105                 110         


Ile Gly Thr Asn Leu Thr Gly Ala Phe Leu Gly Ser Arg Glu Ala Ile 
        115                 120                 125             


Lys Tyr Phe Val Glu Asn Asp Ile Lys Gly Asn Val Ile Asn Met Ser 
    130                 135                 140                 


Ser Val His Glu Val Ile Pro Trp Pro Leu Phe Val His Tyr Ala Ala 
145                 150                 155                 160 


Ser Lys Gly Gly Ile Lys Leu Met Thr Lys Thr Leu Ala Leu Glu Tyr 
                165                 170                 175     


Ala Pro Lys Gly Ile Arg Val Asn Asn Ile Gly Pro Gly Ala Ile Asn 
            180                 185                 190         


Thr Pro Ile Asn Ala Glu Lys Phe Ala Asp Pro Lys Gln Lys Ala Asp 
        195                 200                 205             


Val Glu Ser Met Ile Pro Met Gly Tyr Ile Gly Glu Pro Glu Glu Ile 
    210                 215                 220                 


Ala Ala Val Ala Val Trp Leu Ala Ser Lys Glu Ser Ser Tyr Val Thr 
225                 230                 235                 240 


Gly Ile Thr Leu Phe Ala Asp Gly Gly Met Thr Lys Tyr Pro Ser Phe 
                245                 250                 255     


Gln Ala Gly Arg Gly 
            260     


<210>  2
<211>  260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutated glucose dehydrogenase (E170K, K252L)


<220>
<221>  MUTAGEN
<222>  (170)..(170)
<223>  E170K

<220>
<221>  MUTAGEN
<222>  (252)..(252)
<223>  K252L

<400>  2

Met Tyr Pro Asp Leu Lys Gly Lys Val Val Ala Ile Thr Gly Ala Ala 
1               5                   10                  15      


Ser Gly Leu Gly Lys Ala Met Ala Ile Arg Phe Gly Lys Glu Gln Ala 
            20                  25                  30          


Lys Val Val Ile Asn Tyr Tyr Ser Asn Lys Gln Asp Pro Asn Glu Val 
        35                  40                  45              


Lys Glu Glu Val Ile Lys Ala Gly Gly Glu Ala Val Val Val Gln Gly 
    50                  55                  60                  


Asp Val Thr Lys Glu Glu Asp Val Lys Asn Ile Val Gln Thr Ala Ile 
65                  70                  75                  80  


Lys Glu Phe Gly Thr Leu Asp Ile Met Ile Asn Asn Ala Gly Leu Glu 
                85                  90                  95      


Asn Pro Val Pro Ser His Glu Met Pro Leu Lys Asp Trp Asp Lys Val 
            100                 105                 110         


Ile Gly Thr Asn Leu Thr Gly Ala Phe Leu Gly Ser Arg Glu Ala Ile 
        115                 120                 125             


Lys Tyr Phe Val Glu Asn Asp Ile Lys Gly Asn Val Ile Asn Met Ser 
    130                 135                 140                 


Ser Val His Glu Val Ile Pro Trp Pro Leu Phe Val His Tyr Ala Ala 
145                 150                 155                 160 


Ser Lys Gly Gly Ile Lys Leu Met Thr Lys Thr Leu Ala Leu Glu Tyr 
                165                 170                 175     


Ala Pro Lys Gly Ile Arg Val Asn Asn Ile Gly Pro Gly Ala Ile Asn 
            180                 185                 190         


Thr Pro Ile Asn Ala Glu Lys Phe Ala Asp Pro Lys Gln Lys Ala Asp 
        195                 200                 205             


Val Glu Ser Met Ile Pro Met Gly Tyr Ile Gly Glu Pro Glu Glu Ile 
    210                 215                 220                 


Ala Val Ala Val Trp Leu Ala Ser Lys Glu Ser Ser Tyr Val Thr Gly 
225                 230                 235                 240 


Ile Thr Leu Phe Ala Asp Gly Gly Met Thr Leu Tyr Pro Ser Phe Gln 
                245                 250                 255     


Ala Gly Arg Gly 
            260 


