
                         
                                SEQUENCE LISTING

<110> JANSSEN BIOTECH, INC.
      JANSSEN BIOLOGICS, B.V.
      FLIKWEERT, Marcel  
      GOOCHEE, Charles 
      MASLANKA, Francis C. 
      NAGEL, Franciscus Johannes Ignatius  
      RYLAND, James R 
      SCHAEFER, Eugene 
 

<120> Manufacturing Methods To Control C-Terminal Lysine, 
      Galactose and Sialic Acid Content In Recombinant Proteins
  

<130> JBI5029WOPCT 


<140> Not Assigned  
<141> Not Assigned


<150> 61/791,094
<151> 2013-03-15


<160> 30


<170> FastSEQ for Windows Version 4.0


<210> 1
<211> 157
<212> PRT
<213> Homo sapiens

<400> 1
Val Arg Ser Ser Ser Arg Thr Pro Ser Asp Lys Pro Val Ala His Val
 1               5                  10                  15      
Val Ala Asn Pro Gln Ala Glu Gly Gln Leu Gln Trp Leu Asn Arg Arg
            20                  25                  30          
Ala Asn Ala Leu Leu Ala Asn Gly Val Glu Leu Arg Asp Asn Gln Leu
        35                  40                  45              
Val Val Pro Ser Glu Gly Leu Tyr Leu Ile Tyr Ser Gln Val Leu Phe
    50                  55                  60                  
Lys Gly Gln Gly Cys Pro Ser Thr His Val Leu Leu Thr His Thr Ile
65                  70                  75                  80  
Ser Arg Ile Ala Val Ser Tyr Gln Thr Lys Val Asn Leu Leu Ser Ala
                85                  90                  95      
Ile Lys Ser Pro Cys Gln Arg Glu Thr Pro Glu Gly Ala Glu Ala Lys
            100                 105                 110         
Pro Trp Tyr Glu Pro Ile Tyr Leu Gly Gly Val Phe Gln Leu Glu Lys
        115                 120                 125             
Gly Asp Arg Leu Ser Ala Glu Ile Asn Arg Pro Asp Tyr Leu Asp Phe
    130                 135                 140                 
Ala Glu Ser Gly Gln Val Tyr Phe Gly Ile Ile Ala Leu
145                 150                 155         


<210> 2
<211> 321
<212> DNA
<213> Mus Balb/c

<220> 
<221> CDS            
<222> (1)...(321)

<400> 2
gac atc ttg ctg act cag tct cca gcc atc ctg tct gtg agt cca gga   48
Asp Ile Leu Leu Thr Gln Ser Pro Ala Ile Leu Ser Val Ser Pro Gly
 1               5                   10                  15
 
gaa aga gtc agt ttc tcc tgc agg gcc agt cag ttc gtt ggc tca agc   96
Glu Arg Val Ser Phe Ser Cys Arg Ala Ser Gln Phe Val Gly Ser Ser
             20                  25                  30
 
atc cac tgg tat cag caa aga aca aat ggt tct cca agg ctt ctc ata   144
Ile His Trp Tyr Gln Gln Arg Thr Asn Gly Ser Pro Arg Leu Leu Ile
         35                  40                  45
 
aag tat gct tct gag tct atg tct ggg atc cct tcc agg ttt agt ggc   192
Lys Tyr Ala Ser Glu Ser Met Ser Gly Ile Pro Ser Arg Phe Ser Gly
     50                  55                  60
 
agt gga tca ggg aca gat ttt act ctt agc atc aac act gtg gag tct   240
Ser Gly Ser Gly Thr Asp Phe Thr Leu Ser Ile Asn Thr Val Glu Ser
 65                  70                  75                  80
 
gaa gat att gca gat tat tac tgt caa caa agt cat agc tgg cca ttc   288
Glu Asp Ile Ala Asp Tyr Tyr Cys Gln Gln Ser His Ser Trp Pro Phe
                 85                  90                  95
 
acg ttc ggc tcg ggg aca aat ttg gaa gta aaa                       321
Thr Phe Gly Ser Gly Thr Asn Leu Glu Val Lys
            100                 105
 

<210> 3
<211> 107
<212> PRT
<213> Mus Balb/c

<400> 3
Asp Ile Leu Leu Thr Gln Ser Pro Ala Ile Leu Ser Val Ser Pro Gly
 1               5                  10                  15      
Glu Arg Val Ser Phe Ser Cys Arg Ala Ser Gln Phe Val Gly Ser Ser
            20                  25                  30          
Ile His Trp Tyr Gln Gln Arg Thr Asn Gly Ser Pro Arg Leu Leu Ile
        35                  40                  45              
Lys Tyr Ala Ser Glu Ser Met Ser Gly Ile Pro Ser Arg Phe Ser Gly
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Leu Ser Ile Asn Thr Val Glu Ser
65                  70                  75                  80  
Glu Asp Ile Ala Asp Tyr Tyr Cys Gln Gln Ser His Ser Trp Pro Phe
                85                  90                  95      
Thr Phe Gly Ser Gly Thr Asn Leu Glu Val Lys
            100                 105         


<210> 4
<211> 357
<212> DNA
<213> Mus Balb/c

<220> 
<221> CDS            
<222> (1)...(357)

<400> 4
gaa gtg aag ctt gag gag tct gga gga ggc ttg gtg caa cct gga gga   48
Glu Val Lys Leu Glu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
 1               5                   10                  15
 
tcc atg aaa ctc tcc tgt gtt gcc tct gga ttc att ttc agt aac cac   96
Ser Met Lys Leu Ser Cys Val Ala Ser Gly Phe Ile Phe Ser Asn His
             20                  25                  30
 
tgg atg aac tgg gtc cgc cag tct cca gag aag ggg ctt gag tgg gtt   144
Trp Met Asn Trp Val Arg Gln Ser Pro Glu Lys Gly Leu Glu Trp Val
         35                  40                  45
 
gct gaa att aga tca aaa tct att aat tct gca aca cat tat gcg gag   192
Ala Glu Ile Arg Ser Lys Ser Ile Asn Ser Ala Thr His Tyr Ala Glu
     50                  55                  60
 
tct gtg aaa ggg agg ttc acc atc tca aga gat gat tcc aaa agt gct   240
Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Ser Ala
 65                  70                  75                  80
 
gtc tac ctg caa atg acc gac tta aga act gaa gac act ggc gtt tat   288
Val Tyr Leu Gln Met Thr Asp Leu Arg Thr Glu Asp Thr Gly Val Tyr
                 85                  90                  95
 
tac tgt tcc agg aat tac tac ggt agt acc tac gac tac tgg ggc caa   336
Tyr Cys Ser Arg Asn Tyr Tyr Gly Ser Thr Tyr Asp Tyr Trp Gly Gln
            100                 105                 110
 
ggc acc act ctc aca gtc tcc                                       357
Gly Thr Thr Leu Thr Val Ser
        115
 

<210> 5
<211> 119
<212> PRT
<213> Mus Balb/c

<400> 5
Glu Val Lys Leu Glu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly
 1               5                  10                  15      
Ser Met Lys Leu Ser Cys Val Ala Ser Gly Phe Ile Phe Ser Asn His
            20                  25                  30          
Trp Met Asn Trp Val Arg Gln Ser Pro Glu Lys Gly Leu Glu Trp Val
        35                  40                  45              
Ala Glu Ile Arg Ser Lys Ser Ile Asn Ser Ala Thr His Tyr Ala Glu
    50                  55                  60                  
Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Ser Ala
65                  70                  75                  80  
Val Tyr Leu Gln Met Thr Asp Leu Arg Thr Glu Asp Thr Gly Val Tyr
                85                  90                  95      
Tyr Cys Ser Arg Asn Tyr Tyr Gly Ser Thr Tyr Asp Tyr Trp Gly Gln
            100                 105                 110         
Gly Thr Thr Leu Thr Val Ser
        115                 


<210> 6
<211> 8
<212> PRT
<213> Homo sapiens

<400> 6
Gly Thr Leu Val Thr Val Ser Ser
 1               5              


<210> 7
<211> 7
<212> PRT
<213> Homo sapiens

<400> 7
Gly Thr Lys Leu Glu Ile Lys
 1               5          


<210> 8
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR oligonucleotides
      

<400> 8
cctggatacc tgtgaaaaga                                             20

<210> 9
<211> 27
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR oligonucleotides
      

<400> 9
cctggtacct tagtcaccgt ctcctca                                     27

<210> 10
<211> 27
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR oligonucleotides
      

<400> 10
aatagatatc tccttcaaca cctgcaa                                     27

<210> 11
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR oligonucleotides
      


<400> 11
atcgggacaa agttggaaat a                                           21

<210> 12
<211> 16
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR oligonucleotides
      

<400> 12
ggcggtctgg taccgg                                                 16

<210> 13
<211> 19
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR oligonucleotides
      

<400> 13
gtcaacaaca tagtcatca                                              19

<210> 14
<211> 23
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR oligonucleotides
      

<400> 14
cacaggtgtg tccccaagga aaa                                         23

<210> 15
<211> 18
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR oligonucleotides
      
      

<400> 15
aatctggggt aggcacaa                                               18

<210> 16
<211> 17
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR oligonucleotides
      
      

<400> 16
agtgtgtgtc cccaagg                                                17

<210> 17
<211> 24
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR oligonucleotides
      
      

<400> 17
cacagctgcc cgcccaggtg gcat                                        24

<210> 18
<211> 17
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR oligonucleotides
      
      

<400> 18
gtcgccagtg ctccctt                                                17

<210> 19
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<223> PCR oligonucleotides
      
      

<400> 19
atcggacgtg gacgtgcaga                                             20

<210> 20
<211> 11
<212> PRT
<213> Artificial Sequence

<220> 
<223> Partial sequence of pHC707



<400> 20
Ile Glu Pro Gly Thr Leu Val Thr Val Ser Ser
 1               5                  10      


<210> 21
<211> 46
<212> DNA
<213> Artificial Sequence

<220> 
<223> Partial sequence of pHC707

<400> 21
cacaggtatc caggcctggt accttagtca ccgtctcctc aggtaa                46

<210> 22
<211> 16
<212> DNA
<213> Artificial Sequence

<220> 
<223> Partial sequence of pHC707

<400> 22
cacaggtatc caggca                                                 16

<210> 23
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Partial sequence of pHC707

<400> 23
Pro Gly Thr Leu Val Thr Val Ser Ser
 1               5                  


<210> 24
<211> 32
<212> DNA
<213> Artificial Sequence

<220> 
<223> Partial sequence of pHC707

<400> 24
cctggtacct tagtcaccgt ctcctcaggt aa                               32

<210> 25
<211> 12
<212> PRT
<213> Artificial Sequence

<220> 
<223> Partial sequence of pLC871

<400> 25
Val Glu Gly Asp Ile Gly Thr Lys Leu Glu Ile Lys
 1               5                  10          


<210> 26
<211> 52
<212> DNA
<213> Artificial Sequence

<220> 
<223> Partial sequence of pLC871

<400> 26
tttgcaggtg ttgaaggaga tatcgggaca aagttggaaa taaaacgtaa gt         52

<210> 27
<211> 4
<212> PRT
<213> Artificial Sequence

<220> 
<223> Partial sequence of pLC671

<400> 27
Val Glu Gly Asp
 1              


<210> 28
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<223> Partial sequence of pLC671

<400> 28
tttgcaggtg ttgaaggaga t                                           21

<210> 29
<211> 8
<212> PRT
<213> Artificial Sequence

<220> 
<223> Partial sequence of pLC671

<400> 29
Ile Gly Thr Lys Leu Glu Ile Lys
 1               5              


<210> 30
<211> 31
<212> DNA
<213> Artificial Sequence

<220> 
<223> Partial sequence of pLC671

<400> 30
atcgggacaa agttggaaat aaaacgtaag t                                31
