                               SEQUENCE LISTING

<110> MODERNA THERAPEUTICS, INC.
 
<120> MANUFACTURING METHODS FOR PRODUCTION OF RNA TRANSCRIPTS

<130> 30158-26391/PCT

<140> PCT/US2014/026835
<141> 2014-03-13

<150> 61/800,049
<151> 2013-03-15

<160> 13    

<170> PatentIn version 3.5

<210> 1
<211> 923
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 1
ggggaaauaa gagagaaaag aagaguaaga agaaauauaa gagccaccau ggccgguccc       60

gcgacccaaa gccccaugaa acuuauggcc cugcaguugc ugcuuuggca cucggcccuc      120

uggacagucc aagaagcgac uccucucgga ccugccucau cguugccgca gucauuccuu      180

uugaaguguc uggagcaggu gcgaaagauu cagggcgaug gagccgcacu ccaagagaag      240

cucugcgcga cauacaaacu uugccauccc gaggagcucg uacugcucgg gcacagcuug      300

gggauucccu gggcuccucu cucguccugu ccgucgcagg cuuugcaguu ggcagggugc      360

cuuucccagc uccacuccgg uuuguucuug uaucagggac ugcugcaagc ccuugaggga      420

aucucgccag aauugggccc gacgcuggac acguugcagc ucgacguggc ggauuucgca      480

acaaccaucu ggcagcagau ggaggaacug gggauggcac ccgcgcugca gcccacgcag      540

ggggcaaugc cggccuuugc guccgcguuu cagcgcaggg cggguggagu ccucguagcg      600

agccaccuuc aaucauuuuu ggaagucucg uaccgggugc ugagacaucu ugcgcagccg      660

ugauaagcug ccuucugcgg ggcuugccuu cuggccaugc ccuucuucuc ucccuugcac      720

cuguaccucu uggucuuuga auaaagccug aguaggaagg cggccgcucg agcaugcaaa      780

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      840

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      900

aaaaaaaaaa aaaaaaaauc uag                                              923


<210> 2
<211> 16
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 2
aaaaaaaaaa tctaga                                                       16


<210> 3
<211> 17
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 3
aaaaaaaaaa gaagagc                                                      17


<210> 4
<211> 16
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 4
tctagatttt tttttt                                                       16


<210> 5
<211> 17
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 5
gctcttcttt ttttttt                                                      17


<210> 6
<211> 11
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 6
aaaaaaaaaa t                                                            11


<210> 7
<211> 15
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 7
ctagattttt ttttt                                                        15


<210> 8
<211> 15
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 8
aaaaaaaaaa tctag                                                        15


<210> 9
<211> 3781
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 9
ggtctcctat acgtctctta tacgacatca ccgatgggga acccagacgc tgagtacgta       60

ttctaaatgc ataataaata ctgataacat cttatagttt gtattatatt ttgtattatc      120

gttgacatgt ataattttga tatcaaaaac tgattttccc tttattattt tcgagattta      180

ttttcttaat tctctttaac aaactagaaa tattgtatat acaaaaaatc ataaataata      240

gatgaatagt ttaattatag gtgttcatca atcgaaaaag caacgtatct tatttaaagt      300

gcgttgcttt tttctcattt ataaggttaa ataattctca tatatcaagc aaagtgacag      360

gcgcccttaa atattctgac aaatgctctt tccctaaact ccccccataa aaaaacccgc      420

cgaagcgggt ttttacgtta tttgcggatt aacgattact cgttatcaga accgcccagg      480

gggcccgagc ttaagactgg ccgtcgtttt acaacacaga aagagtttgt agaaacgcaa      540

aaaggccatc cgtcaggggc cttctgctta gtttgatgcc tggcagttcc ctactctcgc      600

cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat      660

cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga      720

acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt      780

ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt      840

ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc      900

gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa      960

gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct     1020

ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta     1080

actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg     1140

gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggg     1200

ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta     1260

ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg     1320

gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt     1380

tgatcttttc tacggggtct gacgctcagt ggaacgacgc gcgcgtaact cacgttaagg     1440

gattttggtc atgagcttgc gccgtcccgt caagtcagcg taatgctctg cttttaccaa     1500

tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc     1560

tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagcgct     1620

gcgatgatac cgcgagaacc acgctcaccg gctccggatt tatcagcaat aaaccagcca     1680

gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt     1740

aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt     1800

gccatcgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc     1860

ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc     1920

tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt     1980

atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact     2040

ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc     2100

ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt     2160

ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg     2220

atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct     2280

gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa     2340

tgttgaatac tcatattctt cctttttcaa tattattgaa gcatttatca gggttattgt     2400

ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggtcagtgtt     2460

acaaccaatt aaccaattct gaacattatc gcgagcccat ttatacctga atatggctca     2520

taacacccct tgtttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa     2580

ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg actccccatg cgagagtagg     2640

gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgcccgg     2700

gctaattagg ggggctggat cgcttcgtgt tccccatcgg tgatgtcgta taggaagcag     2760

tatacgagac ctataggaga cgtatatggt cttcttttct agattttttt tttttttttt     2820

tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt     2880

tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt     2940

ttttgcatgc tcgagcggcc gccttcctac tcaggcttta ttcaaagacc aagaggtaca     3000

ggtgcaaggg agagaagaag ggcatggcca gaaggcaagc cccgcagaag gcagcttatc     3060

acggctgcgc aagatgtctc agcacccggt acgagacttc caaaaatgat tgaaggtggc     3120

tcgctacgag gactccaccc gccctgcgct gaaacgcgga cgcaaaggcc ggcattgccc     3180

cctgcgtggg ctgcagcgcg ggtgccatcc ccagttcctc catctgctgc cagatggttg     3240

ttgcgaaatc cgccacgtcg agctgcaacg tgtccagcgt cgggcccaat tctggcgaga     3300

ttccctcaag ggcttgcagc agtccctgat acaagaacaa accggagtgg agctgggaaa     3360

ggcaccctgc caactgcaaa gcctgcgacg gacaggacga gagaggagcc cagggaatcc     3420

ccaagctgtg cccgagcagt acgagctcct cgggatggca aagtttgtat gtcgcgcaga     3480

gcttctcttg gagtgcggct ccatcgccct gaatctttcg cacctgctcc agacacttca     3540

aaaggaatga ctgcggcaac gatgaggcag gtccgagagg agtcgcttct tggactgtcc     3600

agagggccga gtgccaaagc agcaactgca gggccataag tttcatgggg ctttgggtcg     3660

cgggaccggc catggtggct cttatatttc ttcttactct tcttttctct cttatttccc     3720

tatagtgagt cgtattagct tctgtacgag ggtccaaaag cttgaccccg aagacactat     3780

a                                                                     3781


<210> 10
<211> 4450
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 10
aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa       60

caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt      120

ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc      180

cgtagttagc ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa      240

tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa      300

gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc      360

ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa      420

gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa      480

caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg      540

ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc      600

tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg      660

ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg      720

agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg      780

aagcggaagg cgagagtagg gaactgccag gcatcaaact aagcagaagg cccctgacgg      840

atggcctttt tgcgtttcta caaactcttt ctgtgttgta aaacgacggc cagtcttaag      900

ctcgggcccc ctgggcggtt ctgataacga gtaatcgtta atccgcaaat aacgtaaaaa      960

cccgcttcgg cgggtttttt tatgggggga gtttagggaa agagcatttg tcagaatatt     1020

taagggcgcc tgtcactttg cttgatatat gagaattatt taaccttata aatgagaaaa     1080

aagcaacgca ctttaaataa gatacgttgc tttttcgatt gatgaacacc tataattaaa     1140

ctattcatct attatttatg attttttgta tatacaatat ttctagtttg ttaaagagaa     1200

ttaagaaaat aaatctcgaa aataataaag ggaaaatcag tttttgatat caaaattata     1260

catgtcaacg ataatacaaa atataataca aactataaga tgttatcagt atttattatg     1320

catttagaat aaattttgtg tcgcccttcg ctgaatcaag cttttggacc ctcgtacaga     1380

agctaatacg actcactata gggaaataag agagaaaaga agagtaagaa gaaatataag     1440

agccaccatg cagcgcgtca acatgattat ggccgaatcg ccgggactca tcacaatctg     1500

cctcttgggt tatctcttgt cggcagaatg taccgtgttc ttggatcacg aaaacgcgaa     1560

caaaattctt aatcgcccga agcggtataa ctccgggaaa cttgaggagt ttgtgcaggg     1620

caatcttgaa cgagagtgca tggaggagaa atgctccttt gaggaggcga gggaagtgtt     1680

tgaaaacaca gagcgaacaa cggagttttg gaagcaatac gtagatgggg accagtgtga     1740

gtcgaatccg tgcctcaatg ggggatcatg taaagatgac atcaatagct atgaatgctg     1800

gtgcccgttt gggtttgaag ggaagaactg tgagctggat gtgacgtgca acatcaaaaa     1860

cggacgctgt gagcagtttt gtaagaactc ggctgacaat aaggtagtat gctcgtgcac     1920

agagggatac cggctggcgg agaaccaaaa atcgtgcgag cccgcagtcc cgttcccttg     1980

tgggagggtg agcgtgtcac agactagcaa gttgacgaga gcggagactg tattccccga     2040

cgtggactac gtcaacagca ccgaagccga aacaatcctc gataacatca cgcagagcac     2100

tcagtccttc aatgacttta cgagggtcgt aggtggtgag gacgcgaaac ccggtcagtt     2160

cccctggcag gtggtattga acggaaaagt cgatgccttt tgtggaggtt ccattgtcaa     2220

cgagaagtgg attgtcacag cggcacactg cgtagaaaca ggagtgaaaa tcacggtagt     2280

ggcgggagag cataacattg aagagacaga gcacacggaa caaaagcgaa atgtcatcag     2340

aatcattcca caccataact ataacgcggc aatcaataag tacaatcacg acatcgcact     2400

tttggagctt gacgaacctt tggtgcttaa ttcgtacgtc acccctattt gtattgccga     2460

caaagagtat acaaacatct tcttgaaatt cggctccggg tacgtatcgg gctggggcag     2520

agtgttccat aagggtagat ccgcactggt gttgcaatac ctcagggtgc ccctcgtgga     2580

tcgagccact tgtctgcggt ccaccaaatt cacaatctac aacaatatgt tctgtgcggg     2640

attccatgaa ggtgggagag atagctgcca gggagactca gggggtcccc acgtgacgga     2700

agtcgagggg acgtcatttc tgacgggaat tatctcatgg ggagaggaat gtgcgatgaa     2760

ggggaaatat ggcatctaca ctaaagtgtc acggtatgtc aattggatca aggaaaagac     2820

gaaactcacg tgataatagg ctggagcctc ggtggccatg cttcttgccc cttgggcctc     2880

cccccagccc ctcctcccct tcctgcaccc gtacccccgt ggtctttgaa taaagtctga     2940

gtgggcggca aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3000

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     3060

aaaaaaaaaa aaaaaaaaaa aaaaaaaaag aagagccgtc aatcgagttc gtacctaagg     3120

gcgacacccc ataattagcc cgggcgaaag gcccagtctt tcgactgagc ctttcgtttt     3180

atttgatgcc tggcagttcc ctactctcgc atggggagtc cccacactac catcggcgct     3240

acggcgtttc acttctgagt tcggcatggg gtcaggtggg accaccgcgc tactgccgcc     3300

aggcaaacaa ggggtgttat gagccatatt caggtataaa tgggctcgcg ataatgttca     3360

gaattggtta attggttgta acactgaccc ctatttgttt atttttctaa atacattcaa     3420

atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga     3480

agaatatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc     3540

ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg     3600

gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc     3660

gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat     3720

tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg     3780

acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag     3840

aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa     3900

cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc     3960

gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca     4020

cgatgcctgt agcgatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc     4080

tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc     4140

tgcgctcggc ccttccggct ggctggttta ttgctgataa atccggagcc ggtgagcgtg     4200

gttctcgcgg tatcatcgca gcgctggggc cagatggtaa gccctcccgt atcgtagtta     4260

tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag     4320

gtgcctcact gattaagcat tggtaaaagc agagcattac gctgacttga cgggacggcg     4380

caagctcatg accaaaatcc cttaacgtga gttacgcgcg cgtcgttcca ctgagcgtca     4440

gaccccgtag                                                            4450


<210> 11
<211> 300
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<220>
<221> misc_feature
<222> (1)..(300)
<223> This sequence may encompass 5-300 nucleotides, wherein
      some positions may be absent

<400> 11
aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa       60

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      120

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      180

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      240

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      300


<210> 12
<211> 160
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<220>
<221> misc_feature
<222> (1)..(160)
<223> This sequence may encompass 60-160 nucleotides, wherein
      some positions may be absent

<400> 12
aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa       60

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      120

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa                            160


<210> 13
<211> 120
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 13
aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa       60

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      120


