                         SEQUENCE LISTING

<110>  PUMFORD, Neil
       LAYTON, Sherryll
       HARGIS, Billy
       BIELKE, Lisa
       FAULKNER, Olivia
       ABI-GHANEM, Daad
       BERGHMAN, Luc
 
<120>  COMPOSITIONS AND METHODS OF ENHANCING IMMUNE RESPONSES TO ENTERIC
        PATHOGENS

<130>  5658-00203

<150>  US 61/790,301
<151>  2013-03-15

<160>  46    

<170>  PatentIn version 3.5

<210>  1
<211>  19
<212>  PRT
<213>  E. coli

<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  PAL bis from E. coli

<400>  1

Glu Gly His Ala Asp Glu Arg Gly Thr Pro Glu Tyr Asn Ile Ser Leu 
1               5                   10                  15      


Gly Glu Arg 
            


<210>  2
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide

<400>  2

Gly His Ala Asp Glu Arg 
1               5       


<210>  3
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide

<400>  3

Asp Glu Arg Gly Thr Pro 
1               5       


<210>  4
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide

<400>  4

Glu Tyr Asn Ile Ser Leu 
1               5       


<210>  5
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide

<400>  5

Ile Ser Leu Gly Glu Arg 
1               5       


<210>  6
<211>  19
<212>  PRT
<213>  Vibrio spp.


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  PAL bis from vibrio spp.

<400>  6

Glu Gly His Ala Asp Glu Arg Gly Thr Pro Glu Tyr Asn Ile Ala Leu 
1               5                   10                  15      


Gly Glu Arg 
            


<210>  7
<211>  17
<212>  PRT
<213>  Campylobacter spp.


<220>
<221>  misc_feature
<222>  (1)..(17)
<223>  corresponding peptide from Campylobacter spp.

<400>  7

Glu Gly Asn Cys Asp Glu Trp Gly Thr Asp Glu Tyr Asn Gln Ala Leu 
1               5                   10                  15      


Gly 
    


<210>  8
<211>  19
<212>  PRT
<213>  E. coli


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  PAL from E. coli

<400>  8

Thr Val Glu Gly His Ala Asp Glu Arg Gly Thr Pro Glu Tyr Asn Ile 
1               5                   10                  15      


Ser Leu Gly 
            


<210>  9
<211>  8
<212>  PRT
<213>  Avian Influenza virus m2e




<400>  9

Glu Val Glu Thr Pro Ile Arg Asn 
1               5               


<210>  10
<211>  8
<212>  PRT
<213>  Avian Influenza virus m2e


<400>  10

Glu Val Glu Thr Pro Thr Arg Asn 
1               5               


<210>  11
<211>  12
<212>  PRT
<213>  Avian Influenza virus


<220>
<221>  misc_feature
<222>  (1)..(12)
<223>  Avian Influenza virus (HA5 UA)

<400>  11

Leu Leu Ser Arg Ile Asn His Phe Glu Lys Ile Gln 
1               5                   10          


<210>  12
<211>  19
<212>  PRT
<213>  Avian Influenza virus


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  Avian Influenza virus (HA5 LB)

<400>  12

Ala Asn Pro Ala Asn Asp Leu Cys Tyr Pro Gly Asp Phe Asn Asp Tyr 
1               5                   10                  15      


Glu Glu Leu 
            


<210>  13
<211>  16
<212>  PRT
<213>  Avian Influenza virus


<220>
<221>  misc_feature
<222>  (1)..(16)
<223>  Avian Influenza virus (NP 54-69)

<400>  13

Gly Arg Leu Ile Gln Asn Ser Ile Thr Ile Glu Arg Met Val Leu Ser 
1               5                   10                  15      


<210>  14
<211>  14
<212>  PRT
<213>  Avian Influenza virus


<220>
<221>  misc_feature
<222>  (1)..(14)
<223>  Avian Influenza virus (NP 147-160)

<400>  14

Thr Tyr Gln Arg Thr Arg Ala Leu Val Arg Thr Gly Met Asp 
1               5                   10                  


<210>  15
<211>  190
<212>  PRT
<213>  Gallus gallus


<220>
<221>  misc_feature
<222>  (1)..(190)
<223>  Chicken HMGB1 amino acid

<400>  15

Met Gly Lys Gly Asp Pro Lys Lys Pro Arg Gly Lys Met Ser Ser Tyr 
1               5                   10                  15      


Ala Phe Phe Val Gln Thr Cys Arg Glu Glu His Lys Lys Lys His Pro 
            20                  25                  30          


Asp Ala Ser Val Asn Phe Ser Glu Phe Ser Lys Lys Cys Ser Glu Arg 
        35                  40                  45              


Trp Lys Thr Met Ser Ser Lys Glu Lys Gly Lys Phe Glu Asp Met Ala 
    50                  55                  60                  


Lys Ala Asp Lys Leu Arg Tyr Glu Lys Glu Met Lys Asn Tyr Val Pro 
65                  70                  75                  80  


Pro Lys Gly Glu Thr Lys Lys Lys Phe Lys Asp Pro Asn Ala Pro Lys 
                85                  90                  95      


Arg Pro Pro Ser Ala Phe Phe Leu Phe Cys Ser Glu Phe Arg Pro Lys 
            100                 105                 110         


Ile Lys Gly Glu His Pro Gly Leu Ser Ile Gly Asp Val Ala Lys Lys 
        115                 120                 125             


Leu Gly Glu Met Trp Asn Asn Thr Ala Ala Asp Asp Lys Gln Pro Tyr 
    130                 135                 140                 


Glu Lys Lys Ala Ala Lys Leu Lys Glu Lys Tyr Glu Lys Asp Ile Ala 
145                 150                 155                 160 


Ala Tyr Arg Ala Lys Gly Lys Val Asp Ala Gly Lys Lys Val Val Ala 
                165                 170                 175     


Lys Ala Glu Lys Ser Lys Lys Lys Lys Glu Glu Glu Glu Asp 
            180                 185                 190 


<210>  16
<211>  85
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic HMGB1 box a1

<400>  16

Met Gly Lys Gly Asp Pro Lys Lys Pro Arg Gly Lys Met Ser Ser Tyr 
1               5                   10                  15      


Ala Phe Phe Val Gln Thr Cys Arg Glu Glu His Lys Lys Lys His Pro 
            20                  25                  30          


Asp Ala Ser Val Asn Phe Ser Glu Phe Ser Lys Lys Cys Ser Glu Arg 
        35                  40                  45              


Trp Lys Thr Met Ser Ser Lys Glu Lys Gly Lys Phe Glu Asp Met Ala 
    50                  55                  60                  


Lys Ala Asp Lys Leu Arg Tyr Glu Lys Glu Met Lys Asn Tyr Val Pro 
65                  70                  75                  80  


Pro Lys Gly Glu Thr 
                85  


<210>  17
<211>  54
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic HMGB1 box a2

<400>  17

Pro Asp Ala Ser Val Asn Phe Ser Glu Phe Ser Lys Lys Cys Ser Glu 
1               5                   10                  15      


Arg Trp Lys Thr Met Ser Ser Lys Glu Lys Gly Lys Phe Glu Asp Met 
            20                  25                  30          


Ala Lys Ala Asp Lys Leu Arg Tyr Glu Lys Glu Met Lys Asn Tyr Val 
        35                  40                  45              


Pro Pro Lys Gly Glu Thr 
    50                  


<210>  18
<211>  73
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic HMGB1 box b1

<400>  18

Lys Asp Pro Asn Ala Pro Lys Arg Pro Pro Ser Ala Phe Phe Leu Phe 
1               5                   10                  15      


Cys Ser Glu Phe Arg Pro Lys Ile Lys Gly Glu His Pro Gly Leu Ser 
            20                  25                  30          


Ile Gly Asp Val Ala Lys Lys Leu Gly Glu Met Trp Asn Asn Thr Ala 
        35                  40                  45              


Ala Asp Asp Lys Gln Pro Tyr Glu Lys Lys Ala Ala Lys Leu Lys Glu 
    50                  55                  60                  


Lys Tyr Glu Lys Asp Ile Ala Ala Tyr 
65                  70              


<210>  19
<211>  69
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic HMGB1 box b2

<400>  19

Asn Ala Pro Lys Arg Pro Pro Ser Ala Phe Phe Leu Phe Cys Ser Glu 
1               5                   10                  15      


Phe Arg Pro Lys Ile Lys Gly Glu His Pro Gly Leu Ser Ile Gly Asp 
            20                  25                  30          


Val Ala Lys Lys Leu Gly Glu Met Trp Asn Asn Thr Ala Ala Asp Asp 
        35                  40                  45              


Lys Gln Pro Tyr Glu Lys Lys Ala Ala Lys Leu Lys Glu Lys Tyr Glu 
    50                  55                  60                  


Lys Asp Ile Ala Ala 
65                  


<210>  20
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic HMGB1 RAGE Binding domain

<400>  20

Lys Asp Pro Asn Ala Pro Lys Arg Pro Pro Ser Ala Phe Phe Leu Phe 
1               5                   10                  15      


Cys Ser Glu Phe Arg 
            20      


<210>  21
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic HMGB1 proinflammatory cytokine activity

<400>  21

Leu Lys Glu Lys Tyr Glu Lys Asp Ile Ala Ala Tyr Arg Ala Lys Gly 
1               5                   10                  15      


Lys Val Asp Ala Gly Lys Lys Val Val Ala Lys Ala Glu Lys Ser Lys 
            20                  25                  30          


Lys 
    


<210>  22
<211>  215
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(215)
<223>  HMGB1

<400>  22

Met Gly Lys Gly Asp Pro Lys Lys Pro Arg Gly Lys Met Ser Ser Tyr 
1               5                   10                  15      


Ala Phe Phe Val Gln Thr Cys Arg Glu Glu His Lys Lys Lys His Pro 
            20                  25                  30          


Asp Ala Ser Val Asn Phe Ser Glu Phe Ser Lys Lys Cys Ser Glu Arg 
        35                  40                  45              


Trp Lys Thr Met Ser Ala Lys Glu Lys Gly Lys Phe Glu Asp Met Ala 
    50                  55                  60                  


Lys Ala Asp Lys Ala Arg Tyr Glu Arg Glu Met Lys Thr Tyr Ile Pro 
65                  70                  75                  80  


Pro Lys Gly Glu Thr Lys Lys Lys Phe Lys Asp Pro Asn Ala Pro Lys 
                85                  90                  95      


Arg Pro Pro Ser Ala Phe Phe Leu Phe Cys Ser Glu Tyr Arg Pro Lys 
            100                 105                 110         


Ile Lys Gly Glu His Pro Gly Leu Ser Ile Gly Asp Val Ala Lys Lys 
        115                 120                 125             


Leu Gly Glu Met Trp Asn Asn Thr Ala Ala Asp Asp Lys Gln Pro Tyr 
    130                 135                 140                 


Glu Lys Lys Ala Ala Lys Leu Lys Glu Lys Tyr Glu Lys Asp Ile Ala 
145                 150                 155                 160 


Ala Tyr Arg Ala Lys Gly Lys Pro Asp Ala Ala Lys Lys Gly Val Val 
                165                 170                 175     


Lys Ala Glu Lys Ser Lys Lys Lys Lys Glu Glu Glu Glu Asp Glu Glu 
            180                 185                 190         


Asp Glu Glu Asp Glu Glu Glu Glu Glu Asp Glu Glu Asp Glu Asp Glu 
        195                 200                 205             


Glu Glu Asp Asp Asp Asp Glu 
    210                 215 


<210>  23
<211>  205
<212>  PRT
<213>  Danio rerio


<220>
<221>  misc_feature
<222>  (1)..(205)
<223>  Zebra fish HMGB1

<400>  23

Met Gly Lys Asp Pro Thr Lys Pro Arg Gly Lys Met Ser Ser Tyr Ala 
1               5                   10                  15      


Tyr Phe Val Gln Thr Cys Arg Glu Glu His Lys Lys Lys His Pro Glu 
            20                  25                  30          


Ala Thr Val Asn Phe Ser Glu Phe Ser Lys Lys Cys Ser Glu Arg Trp 
        35                  40                  45              


Lys Thr Met Ser Ala Lys Glu Lys Gly Lys Phe Glu Asp Met Ala Lys 
    50                  55                  60                  


Leu Asp Lys Ala Arg Tyr Glu Arg Glu Met Lys Asn Tyr Ile Pro Pro 
65                  70                  75                  80  


Lys Gly Glu Lys Lys Lys Arg Phe Lys Asp Pro Asn Ala Pro Lys Arg 
                85                  90                  95      


Pro Pro Ser Ala Phe Phe Ile Phe Cys Ser Glu Phe Arg Pro Lys Val 
            100                 105                 110         


Lys Glu Glu Thr Pro Gly Leu Ser Ile Gly Asp Val Ala Lys Arg Leu 
        115                 120                 125             


Gly Glu Met Trp Asn Lys Ile Ser Ser Glu Glu Lys Gln Pro Tyr Glu 
    130                 135                 140                 


Lys Lys Ala Ala Lys Leu Lys Glu Lys Tyr Glu Lys Asp Ile Ala Ala 
145                 150                 155                 160 


Tyr Arg Ser Lys Gly Lys Val Gly Gly Gly Ala Ala Lys Ala Pro Ser 
                165                 170                 175     


Lys Pro Asp Lys Ala Asn Asp Glu Asp Glu Asp Asp Asp Glu Glu Glu 
            180                 185                 190         


Asp Glu Asp Asp Asp Asp Glu Glu Glu Glu Asp Asp Glu 
        195                 200                 205 


<210>  24
<211>  272
<212>  PRT
<213>  Gallus gallus


<220>
<221>  misc_feature
<222>  (1)..(272)
<223>  CD154 chicken

<400>  24

Met Asn Glu Ala Tyr Ser Pro Ala Ala Pro Arg Pro Met Gly Ser Thr 
1               5                   10                  15      


Ser Pro Ser Thr Met Lys Met Phe Met Cys Phe Leu Ser Val Phe Met 
            20                  25                  30          


Val Val Gln Thr Ile Gly Thr Val Leu Phe Cys Leu Tyr Leu His Met 
        35                  40                  45              


Lys Met Asp Lys Met Glu Glu Val Leu Ser Leu Asn Glu Asp Tyr Ile 
    50                  55                  60                  


Phe Leu Arg Lys Val Gln Lys Cys Gln Thr Gly Glu Asp Gln Lys Ser 
65                  70                  75                  80  


Thr Leu Leu Asp Cys Glu Lys Val Leu Lys Gly Phe Gln Asp Leu Gln 
                85                  90                  95      


Cys Lys Asp Arg Thr Ala Ser Glu Glu Leu Pro Lys Phe Glu Met His 
            100                 105                 110         


Arg Gly His Glu His Pro His Leu Lys Ser Arg Asn Glu Thr Ser Val 
        115                 120                 125             


Ala Glu Glu Lys Arg Gln Pro Ile Ala Thr His Leu Ala Gly Val Lys 
    130                 135                 140                 


Ser Asn Thr Thr Val Arg Val Leu Lys Trp Met Thr Thr Ser Tyr Ala 
145                 150                 155                 160 


Pro Thr Ser Ser Leu Ile Ser Tyr His Glu Gly Lys Leu Lys Val Glu 
                165                 170                 175     


Lys Ala Gly Leu Tyr Tyr Ile Tyr Ser Gln Val Ser Phe Cys Thr Lys 
            180                 185                 190         


Ala Ala Ala Ser Ala Pro Phe Thr Leu Tyr Ile Tyr Leu Tyr Leu Pro 
        195                 200                 205             


Met Glu Glu Asp Arg Leu Leu Met Lys Gly Leu Asp Thr His Ser Thr 
    210                 215                 220                 


Ser Thr Ala Leu Cys Glu Leu Gln Ser Ile Arg Glu Gly Gly Val Phe 
225                 230                 235                 240 


Glu Leu Arg Gln Gly Asp Met Val Phe Val Asn Val Thr Asp Ser Thr 
                245                 250                 255     


Ala Val Asn Val Asn Pro Gly Asn Thr Tyr Phe Gly Met Phe Lys Leu 
            260                 265                 270         


<210>  25
<211>  261
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(261)
<223>  Human CD154

<400>  25

Met Ile Glu Thr Tyr Asn Gln Thr Ser Pro Arg Ser Ala Ala Thr Gly 
1               5                   10                  15      


Leu Pro Ile Ser Met Lys Ile Phe Met Tyr Leu Leu Thr Val Phe Leu 
            20                  25                  30          


Ile Thr Gln Met Ile Gly Ser Ala Leu Phe Ala Val Tyr Leu His Arg 
        35                  40                  45              


Arg Leu Asp Lys Ile Glu Asp Glu Arg Asn Leu His Glu Asp Phe Val 
    50                  55                  60                  


Phe Met Lys Thr Ile Gln Arg Cys Asn Thr Gly Glu Arg Ser Leu Ser 
65                  70                  75                  80  


Leu Leu Asn Cys Glu Glu Ile Lys Ser Gln Phe Glu Gly Phe Val Lys 
                85                  90                  95      


Asp Ile Met Leu Asn Lys Glu Glu Thr Lys Lys Glu Asn Ser Phe Glu 
            100                 105                 110         


Met Gln Lys Gly Asp Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser 
        115                 120                 125             


Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly 
    130                 135                 140                 


Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln 
145                 150                 155                 160 


Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr 
                165                 170                 175     


Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser 
            180                 185                 190         


Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala 
        195                 200                 205             


Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His 
    210                 215                 220                 


Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn 
225                 230                 235                 240 


Val Thr Asp Pro Ser Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe 
                245                 250                 255     


Gly Leu Leu Lys Leu 
            260     


<210>  26
<211>  11
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<222>  (1)..(11)
<223>  Human CD154 peptide

<400>  26

Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser Asn 
1               5                   10      


<210>  27
<211>  11
<212>  PRT
<213>  Galus gallus


<220>
<221>  misc_feature
<222>  (1)..(11)
<223>  Chicken CD154 peptide

<400>  27

Trp Met Thr Thr Ser Tyr Ala Pro Thr Ser Ser 
1               5                   10      


<210>  28
<211>  10
<212>  PRT
<213>  Anas sp.


<220>
<221>  misc_feature
<222>  (1)..(10)
<223>  Duck CD154 peptide

<400>  28

Trp Asn Lys Thr Ser Tyr Ala Pro Met Asn 
1               5                   10  


<210>  29
<211>  10
<212>  PRT
<213>  Mus sp.


<220>
<221>  misc_feature
<222>  (1)..(10)
<223>  Mouse CD154 peptide

<400>  29

Trp Ala Lys Lys Gly Tyr Tyr Thr Met Lys 
1               5                   10  


<210>  30
<211>  10
<212>  PRT
<213>  Bos taurus


<220>
<221>  misc_feature
<222>  (1)..(10)
<223>  Cow CD154 peptide

<400>  30

Trp Ala Pro Lys Gly Tyr Tyr Thr Leu Ser 
1               5                   10  


<210>  31
<211>  21
<212>  PRT
<213>  Campylobacter jejuni Cj0113

<400>  31

Gly Val Ser Ile Thr Val Glu Gly Asn Cys Asp Glu Trp Gly Thr Asp 
1               5                   10                  15      


Glu Tyr Asn Gln Ala 
            20      


<210>  32
<211>  19
<212>  PRT
<213>  Vibrio spp.


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  Vibrio spp. alternative PAL epitope

<400>  32

Thr Val Glu Gly His Ala Asp Glu Arg Gly Thr Pro Glu Tyr Asn Ile 
1               5                   10                  15      


Ala Leu Gly 
            


<210>  33
<211>  57
<212>  DNA
<213>  E. coli


<220>
<221>  misc_feature
<222>  (1)..(57)
<223>  E. coli nucleotide sequence for PAL epitope

<400>  33
gaaggtcacg cggacgaacg tggtaccccg gaatacaaca tctctctggg tgaacgt          57


<210>  34
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic nucleotide sequence encoding in vector 
       (CJ0113-HMGB1-PAL)

<400>  34
gaaggtcacg cggacgaacg tggtaccccg aatacaacat ctctctgggt gaacgt           56


<210>  35
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide sequence encoded by SEQ ID NO: 34 mutant PAL

<400>  35

Glu Gly His Ala Asp Glu Arg Gly Thr Pro Asn Thr Thr Ser Leu Trp 
1               5                   10                  15      


Val Asn 
        


<210>  36
<211>  9
<212>  PRT
<213>  E. coli


<220>
<221>  misc_feature
<222>  (1)..(9)
<223>  Epitope of PAL from E. coli

<400>  36

Glu Tyr Asn Ile Ser Leu Gly Glu Arg 
1               5                   


<210>  37
<211>  9
<212>  PRT
<213>  Vibrio spp.


<220>
<221>  misc_feature
<222>  (1)..(9)
<223>  Epitope of PAL from Vibrio spp.

<400>  37

Glu Tyr Asn Ile Ala Leu Gly Glu Arg 
1               5                   


<210>  38
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide

<400>  38

Tyr Lys Val Thr Val Glu 
1               5       


<210>  39
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide

<400>  39

Thr Val Glu Gly His Ala 
1               5       


<210>  40
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide

<400>  40

Gly Thr Pro Glu Tyr Asn 
1               5       


<210>  41
<211>  726
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic insert in CJ0113-PAL-HMGB1 nucleotide

<400>  41
tcctcctccg gtgtttctat caccgttgaa ggtaactgcg acgaatgggg taccgacgaa       60

tacaaccagg cgtcctcctc cgaaggtcac gcggacgaac gtggtacccc ggaatacaac      120

atctctctgg gtgaacgttc ctcctccatg ggtaaaggcg acccgaaaaa accgcgtggt      180

aaaatgtctt cttacgcgtt cttcgttcag acctgccgtg aagaacacaa aaaaaaacac      240

ccggacgctt ctgttaactt ctctgaattc tctaaaaaat gctctgaaag atggaaaacc      300

atgtcttcta aagaaaaagg taaattcgaa gacatggcga aagcggacaa actgagatac      360

gaaaaagaaa tgaaaaacta cgttccgccg aaaggtgaaa ccaaaaaaaa attcaaagac      420

ccgaacgcgc cgaaacgtcc gccgtctgcg ttcttcctgt tctgcagcga attcagaccg      480

aaaatcaaag gtgaacaccc gggtctgtct atcggtgacg ttgcgaaaaa actgggtgaa      540

atgtggaaca acaccgcggc ggacgacaaa cagccgtacg aaaaaaaagc ggcgaaactg      600

aaagaaaaat acgaaaaaga catcgcggcg tacagagcga aaggtaaagt tgacgcgggt      660

aaaaaagttg ttgcgaaagc ggaaaaatct aaaaaaaaaa aagaagaaga agaagactcc      720

tcctcc                                                                 726


<210>  42
<211>  242
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic insert in CJ0113-PAL-HMGB1 amino acid

<400>  42

Ser Ser Ser Gly Val Ser Ile Thr Val Glu Gly Asn Cys Asp Glu Trp 
1               5                   10                  15      


Gly Thr Asp Glu Tyr Asn Gln Ala Ser Ser Ser Glu Gly His Ala Asp 
            20                  25                  30          


Glu Arg Gly Thr Pro Glu Tyr Asn Ile Ser Leu Gly Glu Arg Ser Ser 
        35                  40                  45              


Ser Met Gly Lys Gly Asp Pro Lys Lys Pro Arg Gly Lys Met Ser Ser 
    50                  55                  60                  


Tyr Ala Phe Phe Val Gln Thr Cys Arg Glu Glu His Lys Lys Lys His 
65                  70                  75                  80  


Pro Asp Ala Ser Val Asn Phe Ser Glu Phe Ser Lys Lys Cys Ser Glu 
                85                  90                  95      


Arg Trp Lys Thr Met Ser Ser Lys Glu Lys Gly Lys Phe Glu Asp Met 
            100                 105                 110         


Ala Lys Ala Asp Lys Leu Arg Tyr Glu Lys Glu Met Lys Asn Tyr Val 
        115                 120                 125             


Pro Pro Lys Gly Glu Thr Lys Lys Lys Phe Lys Asp Pro Asn Ala Pro 
    130                 135                 140                 


Lys Arg Pro Pro Ser Ala Phe Phe Leu Phe Cys Ser Glu Phe Arg Pro 
145                 150                 155                 160 


Lys Ile Lys Gly Glu His Pro Gly Leu Ser Ile Gly Asp Val Ala Lys 
                165                 170                 175     


Lys Leu Gly Glu Met Trp Asn Asn Thr Ala Ala Asp Asp Lys Gln Pro 
            180                 185                 190         


Tyr Glu Lys Lys Ala Ala Lys Leu Lys Glu Lys Tyr Glu Lys Asp Ile 
        195                 200                 205             


Ala Ala Tyr Arg Ala Lys Gly Lys Val Asp Ala Gly Lys Lys Val Val 
    210                 215                 220                 


Ala Lys Ala Glu Lys Ser Lys Lys Lys Lys Glu Glu Glu Glu Asp Ser 
225                 230                 235                 240 


Ser Ser 
        


<210>  43
<211>  726
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic insert in CJ0113-HMGB1-PAL nucleotide CJ0113-HMGB1-PAL

<400>  43
tcctcctccg gtgtttctat caccgttgaa ggtaactgcg acgaatgggg taccgacgaa       60

tacaaccagg cgtcctcctc catgggtaaa ggcgacccga aaaaaccgcg tggtaaaatg      120

tcttcttacg cgttcttcgt tcagacctgc cgtgaagaac acaaaaaaaa acacccggac      180

gcttctgtta acttctctga attctctaaa aaatgctctg aaagatggaa aaccatgtct      240

tctaaagaaa aaggtaaatt cgaagacatg gcgaaagcgg acaaactgag atacgaaaaa      300

gaaatgaaaa actacgttcc gccgaaaggt gaaaccaaaa aaaaattcaa agacccgaac      360

gcgccgaaac gtccgccgtc tgcgttcttc ctgttctgca gcgaattcag accgaaaatc      420

aaaggtgaac acccgggtct gtctatcggt gacgttgcga aaaaactggg tgaaatgtgg      480

aacaacaccg cggcggacga caaacagccg tacgaaaaaa aagcggcgaa actgaaagaa      540

aaatacgaaa aagacatcgc ggcgtacaga gcgaaaggta aagttgacgc gggtaaaaaa      600

gttgttgcga aagcggaaaa atctaaaaaa aaaaaagaag aagaagaaga ctcctcctcc      660

gaaggtcacg cggacgaacg tggtaccccg gaatacaaca tctctctggg tgaacgttcc      720

tcctcc                                                                 726


<210>  44
<211>  242
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic insert in CJ0113-HMGB1-PAL amino acid

<400>  44

Ser Ser Ser Gly Val Ser Ile Thr Val Glu Gly Asn Cys Asp Glu Trp 
1               5                   10                  15      


Gly Thr Asp Glu Tyr Asn Gln Ala Ser Ser Ser Met Gly Lys Gly Asp 
            20                  25                  30          


Pro Lys Lys Pro Arg Gly Lys Met Ser Ser Tyr Ala Phe Phe Val Gln 
        35                  40                  45              


Thr Cys Arg Glu Glu His Lys Lys Lys His Pro Asp Ala Ser Val Asn 
    50                  55                  60                  


Phe Ser Glu Phe Ser Lys Lys Cys Ser Glu Arg Trp Lys Thr Met Ser 
65                  70                  75                  80  


Ser Lys Glu Lys Gly Lys Phe Glu Asp Met Ala Lys Ala Asp Lys Leu 
                85                  90                  95      


Arg Tyr Glu Lys Glu Met Lys Asn Tyr Val Pro Pro Lys Gly Glu Thr 
            100                 105                 110         


Lys Lys Lys Phe Lys Asp Pro Asn Ala Pro Lys Arg Pro Pro Ser Ala 
        115                 120                 125             


Phe Phe Leu Phe Cys Ser Glu Phe Arg Pro Lys Ile Lys Gly Glu His 
    130                 135                 140                 


Pro Gly Leu Ser Ile Gly Asp Val Ala Lys Lys Leu Gly Glu Met Trp 
145                 150                 155                 160 


Asn Asn Thr Ala Ala Asp Asp Lys Gln Pro Tyr Glu Lys Lys Ala Ala 
                165                 170                 175     


Lys Leu Lys Glu Lys Tyr Glu Lys Asp Ile Ala Ala Tyr Arg Ala Lys 
            180                 185                 190         


Gly Lys Val Asp Ala Gly Lys Lys Val Val Ala Lys Ala Glu Lys Ser 
        195                 200                 205             


Lys Lys Lys Lys Glu Glu Glu Glu Asp Ser Ser Ser Glu Gly His Ala 
    210                 215                 220                 


Asp Glu Arg Gly Thr Pro Glu Tyr Asn Ile Ser Leu Gly Glu Arg Ser 
225                 230                 235                 240 


Ser Ser 
        


<210>  45
<211>  726
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic insert in HMGB1-CJ0113-PAL nucleotide

<400>  45
tcctcctcca tgggtaaagg cgacccgaaa aaaccgcgtg gtaaaatgtc ttcttacgcg       60

ttcttcgttc agacctgccg tgaagaacac aaaaaaaaac acccggacgc ttctgttaac      120

ttctctgaat tctctaaaaa atgctctgaa agatggaaaa ccatgtcttc taaagaaaaa      180

ggtaaattcg aagacatggc gaaagcggac aaactgagat acgaaaaaga aatgaaaaac      240

tacgttccgc cgaaaggtga aaccaaaaaa aaattcaaag acccgaacgc gccgaaacgt      300

ccgccgtctg cgttcttcct gttctgcagc gaattcagac cgaaaatcaa aggtgaacac      360

ccgggtctgt ctatcggtga cgttgcgaaa aaactgggtg aaatgtggaa caacaccgcg      420

gcggacgaca aacagccgta cgaaaaaaaa gcggcgaaac tgaaagaaaa atacgaaaaa      480

gacatcgcgg cgtacagagc gaaaggtaaa gttgacgcgg gtaaaaaagt tgttgcgaaa      540

gcggaaaaat ctaaaaaaaa aaaagaagaa gaagaagact cctcctccgg tgtttctatc      600

accgttgaag gtaactgcga cgaatggggt accgacgaat acaaccaggc gtcctcctcc      660

gaaggtcacg cggacgaacg tggtaccccg gaatacaaca tctctctggg tgaacgttcc      720

tcctcc                                                                 726


<210>  46
<211>  182
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic insert in HMGB1-CJ0113-PAL amino acid

<400>  46

Ser Ser Ser Met Gly Lys Gly Asp Pro Lys Lys Pro Arg Gly Lys Met 
1               5                   10                  15      


Ser Ser Tyr Ala Phe Phe Val Gln Thr Cys Arg Glu Glu His Lys Lys 
            20                  25                  30          


Lys His Pro Asp Ala Ser Val Asn Phe Ser Glu Phe Ser Lys Lys Cys 
        35                  40                  45              


Ser Glu Arg Trp Lys Thr Met Ser Ser Lys Glu Lys Gly Lys Phe Glu 
    50                  55                  60                  


Asp Met Ala Lys Ala Asp Lys Leu Arg Tyr Glu Lys Glu Met Lys Asn 
65                  70                  75                  80  


Tyr Val Pro Pro Lys Gly Glu Thr Lys Lys Lys Phe Lys Asp Pro Asn 
                85                  90                  95      


Ala Pro Lys Arg Pro Pro Ser Ala Phe Phe Leu Phe Cys Ser Glu Phe 
            100                 105                 110         


Arg Pro Lys Ile Lys Gly Glu His Ala Glu Lys Ser Lys Lys Lys Lys 
        115                 120                 125             


Glu Glu Glu Glu Asp Ser Ser Ser Gly Val Ser Ile Thr Val Glu Gly 
    130                 135                 140                 


Asn Cys Asp Glu Trp Gly Thr Asp Glu Tyr Asn Gln Ala Ser Ser Ser 
145                 150                 155                 160 


Glu Gly His Ala Asp Glu Arg Gly Thr Pro Glu Tyr Asn Ile Ser Leu 
                165                 170                 175     


Gly Glu Arg Ser Ser Ser 
            180         


