                               SEQUENCE LISTING

<110> LIFE TECHNOLOGIES CORPORATION
      GENEART AG
 
<120> HIGH EFFICIENCY, SMALL VOLUME NUCLEIC ACID SYNTHESIS

<130> LT00586.1 PCT

<140> PCT/US2014/029500
<141> 2014-03-14

<150> 61/784,752
<151> 2013-03-14

<160> 44    

<170> PatentIn version 3.5

<210> 1
<211> 10
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 1
atcgcatgcg                                                              10


<210> 2
<211> 15
<212> DNA
<213> Enterobacteria phage lambda

<400> 2
gcttttttat actaa                                                        15


<210> 3
<211> 52
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 3
gaggtaccgt caatttaatt aattttcagg atgacgcgga acttgccaca cg               52


<210> 4
<211> 53
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 4
tgaaattctt ggctattaca acagactgcc ttcagatctt agcttatggc aat              53


<210> 5
<211> 53
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 5
caagagagca agctcatcat tctggccagt ggtggacccc aagccttagt aaa              53


<210> 6
<211> 53
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 6
cataatgagg acctacactt atgagaagct tctgtggacc acaagcagag tgc              53


<210> 7
<211> 53
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 7
tgaaggtgct gtctgtctgc tctagcaaca agccggccat tgtagaagct ggt              53


<210> 8
<211> 53
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 8
gggatgcagg cactggggct tcatctgaca gacccaagtc agcgacttgt tca              53


<210> 9
<211> 53
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 9
aaactgtctt tggactctca gaaacctttc agatgcagcg actaagcagg aag              53


<210> 10
<211> 53
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 10
ggatggaagg cctccttggg actctagtgc attgtcttct tggcgcgccc aac              53


<210> 11
<211> 52
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 11
tgcaattcct gaggcgacaa aagcgctaaa cgatgaggcc caggtggtag tt               52


<210> 12
<211> 52
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 12
aataaagctg ctgttatggt ccatcagctt tccaaaaagg aagcttccag ac               52


<210> 13
<211> 52
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 13
atgccatcat gcgctcccct cagatggtgt ctgccattgt acgcaccatg ca               52


<210> 14
<211> 52
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 14
gaatacaaat gatgtagaga cagctcgttg tactgctggg actctgcaca ac               52


<210> 15
<211> 52
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 15
ctttctcacc accgcgaggg cttgctggcc atctttaagt ctggtggcat cc               52


<210> 16
<211> 53
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 16
cagcgctggt gaaaatgctt gggtcaccag tggattctgt actgttctac gcc              53


<210> 17
<211> 53
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 17
atcacgacac tgcataatct cctgctccat caggaaggag ctaaaatggc agt              53


<210> 18
<211> 53
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 18
gcgcctagct ggtggactgc agaaaatggt tgctttgctc aacaaaacaa acg              53


<210> 19
<211> 34
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 19
tcgcctcagg aattgcacgt gtggcaagtt ccgc                                   34


<210> 20
<211> 40
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 20
tgatgagctt gctctcttga ttgccataag ctaagatctg                             40


<210> 21
<211> 43
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 21
cataagtgta ggtcctcatt atgtttacta aggcttgggg tcc                         43


<210> 22
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 22
cagacagaca gcaccttcag cactctgctt gtggtcc                                37


<210> 23
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 23
cccagtgcct gcatcccacc agcttctaca atggcc                                 36


<210> 24
<211> 40
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 24
ctgagagtcc aaagacagtt ttgaacaagt cgctgacttg                             40


<210> 25
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 25
caaggaggcc ttccatccct tcctgcttag tcgctg                                 36


<210> 26
<211> 40
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 26
ggaccataac agcagcttta ttaactacca cctgggcctc                             40


<210> 27
<211> 38
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 27
ggagcgcatg atggcatgtc tggaagcttc ctttttgg                               38


<210> 28
<211> 43
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 28
gctgtctcta catcatttgt attctgcatg gtgcgtacaa tgg                         43


<210> 29
<211> 35
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 29
tcgcggtggt gagaaaggtt gtgcagagtc ccagc                                  35


<210> 30
<211> 38
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 30
gcattttcac cagcgctggg atgccaccag acttaaag                               38


<210> 31
<211> 42
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 31
gagattatgc agtgtcgtga tggcgtagaa cagtacagaa tc                          42


<210> 32
<211> 35
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 32
ccaccagcta ggcgcactgc cattttagct ccttc                                  35


<210> 33
<211> 45
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 33
ctgttgtaat agccaagaat ttcacgtttg ttttgttgag caaag                       45


<210> 34
<211> 38
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 34
gtcttgtgct ccaggcgcgc caagaagaca atgcacta                               38


<210> 35
<211> 38
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 35
cgtgtcttgt ccaggtaccg tcaatttaat taattttc                               38


<210> 36
<211> 69
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 36
acccgtaaag cgagtttagt tttgaaaaac aaatgacata atgacatcat cccctgattg       60

tgttttaca                                                               69


<210> 37
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 37
aattctaccc gtaaagcgag tttagttttg aaaaac                                 36


<210> 38
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 38
aatgacatca tcccctgatt gtgttttaca agtaga                                 36


<210> 39
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<220>
<221> source
<223> /note="Description of Combined DNA/RNA Molecule: Synthetic
      oligonucleotide"


<220>
<221> CDS
<222> (1)..(33)

<400> 39
ttc ctt cct gct act ggt ggc gtt ttc cgu aat                             33
Phe Leu Pro Ala Thr Gly Gly Val Phe Arg Asn                               
1               5                   10                                    


<210> 40
<211> 11
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<220>
<221> source
<223> /note="Description of Combined DNA/RNA Molecule: Synthetic
      oligonucleotide"

<400> 40
Phe Leu Pro Ala Thr Gly Gly Val Phe Arg Asn 
1               5                   10      


<210> 41
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> CDS
<222> (1)..(33)

<400> 41
ttc ctc ccc gcc acc ggc ggc gtc ttc aga aat                             33
Phe Leu Pro Ala Thr Gly Gly Val Phe Arg Asn                               
1               5                   10                                    


<210> 42
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> CDS
<222> (1)..(33)

<400> 42
ttc cta cca gcc act gga ggc gtc ttc agg aat                             33
Phe Leu Pro Ala Thr Gly Gly Val Phe Arg Asn                               
1               5                   10                                    


<210> 43
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> CDS
<222> (1)..(33)

<400> 43
ttc ctt ccg gca aca ggt ggg gtg ttc cgc aat                             33
Phe Leu Pro Ala Thr Gly Gly Val Phe Arg Asn                               
1               5                   10                                    


<210> 44
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<220>
<221> source
<223> /note="Description of Combined DNA/RNA Molecule: Synthetic
      oligonucleotide"


<220>
<221> CDS
<222> (1)..(33)

<400> 44
ttc ctt ccc gcg acc ggt ggg gta ttc cgu aac                             33
Phe Leu Pro Ala Thr Gly Gly Val Phe Arg Asn                               
1               5                   10                                    


