               SEQUENCE LISTING

<110> BIORON GmbH
      Ignatov, Konstantin
      Kramarov, Vladimir

<120> Novel DNA-Polymerases

<150> US61/808,813
<151> 2013-04-05
<150> EP13162444.7
<151> 2013-04-05

<160> 23

<170> BiSSAP 1.2

<210> 1
<211> 832
<212> PRT
<213> Artificial Sequence

<220> 
<223> Amino acid sequence of wild-type Thermus aquaticus (Taq)
      DNA-polymerase (SEQ ID NO: 1)

<400> 1
Met Arg Gly Met Leu Pro Leu Phe Glu Pro Lys Gly Arg Val Leu Leu 
1               5                   10                  15      
Val Asp Gly His His Leu Ala Tyr Arg Thr Phe His Ala Leu Lys Gly 
            20                  25                  30          
Leu Thr Thr Ser Arg Gly Glu Pro Val Gln Ala Val Tyr Gly Phe Ala 
        35                  40                  45              
Lys Ser Leu Leu Lys Ala Leu Lys Glu Asp Gly Asp Ala Val Ile Val 
    50                  55                  60                  
Val Phe Asp Ala Lys Ala Pro Ser Phe Arg His Glu Ala Tyr Gly Gly 
65                  70                  75                  80  
Tyr Lys Ala Gly Arg Ala Pro Thr Pro Glu Asp Phe Pro Arg Gln Leu 
                85                  90                  95      
Ala Leu Ile Lys Glu Leu Val Asp Leu Leu Gly Leu Ala Arg Leu Glu 
            100                 105                 110         
Val Pro Gly Tyr Glu Ala Asp Asp Val Leu Ala Ser Leu Ala Lys Lys 
        115                 120                 125             
Ala Glu Lys Glu Gly Tyr Glu Val Arg Ile Leu Thr Ala Asp Lys Asp 
    130                 135                 140                 
Leu Tyr Gln Leu Leu Ser Asp Arg Ile His Val Leu His Pro Glu Gly 
145                 150                 155                 160 
Tyr Leu Ile Thr Pro Ala Trp Leu Trp Glu Lys Tyr Gly Leu Arg Pro 
                165                 170                 175     
Asp Gln Trp Ala Asp Tyr Arg Ala Leu Thr Gly Asp Glu Ser Asp Asn 
            180                 185                 190         
Leu Pro Gly Val Lys Gly Ile Gly Glu Lys Thr Ala Arg Lys Leu Leu 
        195                 200                 205             
Glu Glu Trp Gly Ser Leu Glu Ala Leu Leu Lys Asn Leu Asp Arg Leu 
    210                 215                 220                 
Lys Pro Ala Ile Arg Glu Lys Ile Leu Ala His Met Asp Asp Leu Lys 
225                 230                 235                 240 
Leu Ser Trp Asp Leu Ala Lys Val Arg Thr Asp Leu Pro Leu Glu Val 
                245                 250                 255     
Asp Phe Ala Lys Arg Arg Glu Pro Asp Arg Glu Arg Leu Arg Ala Phe 
            260                 265                 270         
Leu Glu Arg Leu Glu Phe Gly Ser Leu Leu His Glu Phe Gly Leu Leu 
        275                 280                 285             
Glu Ser Pro Lys Ala Leu Glu Glu Ala Pro Trp Pro Pro Pro Glu Gly 
    290                 295                 300                 
Ala Phe Val Gly Phe Val Leu Ser Arg Lys Glu Pro Met Trp Ala Asp 
305                 310                 315                 320 
Leu Leu Ala Leu Ala Ala Ala Arg Gly Gly Arg Val His Arg Ala Pro 
                325                 330                 335     
Glu Pro Tyr Lys Ala Leu Arg Asp Leu Lys Glu Ala Arg Gly Leu Leu 
            340                 345                 350         
Ala Lys Asp Leu Ser Val Leu Ala Leu Arg Glu Gly Leu Gly Leu Pro 
        355                 360                 365             
Pro Gly Asp Asp Pro Met Leu Leu Ala Tyr Leu Leu Asp Pro Ser Asn 
    370                 375                 380                 
Thr Thr Pro Glu Gly Val Ala Arg Arg Tyr Gly Gly Glu Trp Thr Glu 
385                 390                 395                 400 
Glu Ala Gly Glu Arg Ala Ala Leu Ser Glu Arg Leu Phe Ala Asn Leu 
                405                 410                 415     
Trp Gly Arg Leu Glu Gly Glu Glu Arg Leu Leu Trp Leu Tyr Arg Glu 
            420                 425                 430         
Val Glu Arg Pro Leu Ser Ala Val Leu Ala His Met Glu Ala Thr Gly 
        435                 440                 445             
Val Arg Leu Asp Val Ala Tyr Leu Arg Ala Leu Ser Leu Glu Val Ala 
    450                 455                 460                 
Glu Glu Ile Ala Arg Leu Glu Ala Glu Val Phe Arg Leu Ala Gly His 
465                 470                 475                 480 
Pro Phe Asn Leu Asn Ser Arg Asp Gln Leu Glu Arg Val Leu Phe Asp 
                485                 490                 495     
Glu Leu Gly Leu Pro Ala Ile Gly Lys Thr Glu Lys Thr Gly Lys Arg 
            500                 505                 510         
Ser Thr Ser Ala Ala Val Leu Glu Ala Leu Arg Glu Ala His Pro Ile 
        515                 520                 525             
Val Glu Lys Ile Leu Gln Tyr Arg Glu Leu Thr Lys Leu Lys Ser Thr 
    530                 535                 540                 
Tyr Ile Asp Pro Leu Pro Asp Leu Ile His Pro Arg Thr Gly Arg Leu 
545                 550                 555                 560 
His Thr Arg Phe Asn Gln Thr Ala Thr Ala Thr Gly Arg Leu Cys Cys 
                565                 570                 575     
Cys Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Thr Pro Leu Gly Gln 
            580                 585                 590         
Arg Ile Arg Arg Gly Phe Ile Ala Glu Glu Gly Trp Leu Leu Val Ala 
        595                 600                 605             
Leu Asp Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Leu Ser Gly 
    610                 615                 620                 
Asp Glu Asn Leu Ile Arg Val Phe Gln Glu Gly Arg Asp Ile His Thr 
625                 630                 635                 640 
Glu Thr Ala Ser Trp Met Phe Gly Val Pro Arg Glu Ala Val Asp Pro 
                645                 650                 655     
Leu Met Arg Arg Ala Ala Lys Thr Ile Asn Phe Gly Val Leu Tyr Gly 
            660                 665                 670         
Met Ser Ala His Arg Leu Ser Gln Glu Leu Ala Ile Pro Tyr Glu Glu 
        675                 680                 685             
Ala Gln Ala Phe Ile Glu Arg Tyr Phe Gln Ser Phe Pro Lys Val Arg 
    690                 695                 700                 
Ala Trp Ile Glu Lys Thr Leu Glu Glu Gly Arg Arg Arg Gly Tyr Val 
705                 710                 715                 720 
Glu Thr Leu Phe Gly Arg Arg Arg Tyr Val Pro Asp Leu Glu Ala Arg 
                725                 730                 735     
Val Lys Ser Val Arg Glu Ala Ala Glu Arg Met Ala Phe Asn Met Pro 
            740                 745                 750         
Val Gln Gly Thr Ala Ala Asp Leu Met Lys Leu Ala Met Val Lys Leu 
        755                 760                 765             
Phe Pro Arg Leu Glu Glu Met Gly Ala Arg Met Leu Leu Gln Val His 
    770                 775                 780                 
Asp Glu Leu Val Leu Glu Ala Pro Lys Glu Arg Ala Glu Ala Val Ala 
785                 790                 795                 800 
Arg Leu Ala Lys Glu Val Met Glu Gly Val Tyr Pro Leu Ala Val Pro 
                805                 810                 815     
Leu Glu Val Glu Val Gly Ile Gly Glu Asp Trp Leu Ser Ala Lys Glu 
            820                 825                 830         



<210> 2
<211> 2244
<212> PRT
<213> Artificial Sequence

<220> 
<223> Amino acid sequence of the improved DNA-Polymerase variant SD DNA
      Polymerase(SEQ ID NO:2)

<400> 2
Met Glu Thr Ala Arg Gly Gly Leu Tyr Met Glu Thr Leu Glu Pro Arg 
1               5                   10                  15      
Leu Glu Pro His Glu Gly Leu Pro Arg Leu Tyr Ser Gly Leu Tyr Ala 
            20                  25                  30          
Arg Gly Val Ala Leu Leu Glu Leu Glu Val Ala Leu Ala Ser Pro Gly 
        35                  40                  45              
Leu Tyr His Ile Ser His Ile Ser Leu Glu Ala Leu Ala Thr Tyr Arg 
    50                  55                  60                  
Ala Arg Gly Thr His Arg Pro His Glu His Ile Ser Ala Leu Ala Leu 
65                  70                  75                  80  
Glu Leu Tyr Ser Gly Leu Tyr Leu Glu Thr His Arg Thr His Arg Ser 
                85                  90                  95      
Glu Arg Ala Arg Gly Gly Leu Tyr Gly Leu Pro Arg Val Ala Leu Gly 
            100                 105                 110         
Leu Asn Ala Leu Ala Val Ala Leu Thr Tyr Arg Ala Ser Pro Pro His 
        115                 120                 125             
Glu Ala Leu Ala Leu Tyr Ser Ser Glu Arg Leu Glu Leu Glu Leu Tyr 
    130                 135                 140                 
Ser Ala Leu Ala Leu Glu Leu Tyr Ser Gly Leu Ala Ser Pro Gly Leu 
145                 150                 155                 160 
Tyr Ala Ser Pro Ala Leu Ala Val Ala Leu Ile Leu Glu Val Ala Leu 
                165                 170                 175     
Val Ala Leu Pro His Glu Ala Ser Pro Ala Leu Ala Leu Tyr Ser Ala 
            180                 185                 190         
Leu Ala Pro Arg Ser Glu Arg Pro His Glu Ala Arg Gly His Ile Ser 
        195                 200                 205             
Gly Leu Ala Leu Ala Thr Tyr Arg Gly Leu Tyr Gly Leu Tyr Thr Tyr 
    210                 215                 220                 
Arg Leu Tyr Ser Ala Leu Ala Gly Leu Tyr Ala Arg Gly Ala Leu Ala 
225                 230                 235                 240 
Pro Arg Thr His Arg Pro Arg Gly Leu Ala Ser Pro Pro His Glu Pro 
                245                 250                 255     
Arg Ala Arg Gly Gly Leu Asn Leu Glu Ala Leu Ala Leu Glu Ile Leu 
            260                 265                 270         
Glu Leu Tyr Ser Gly Leu Leu Glu Val Ala Leu Ala Ser Pro Leu Glu 
        275                 280                 285             
Leu Glu Gly Leu Tyr Leu Glu Ala Leu Ala Ala Arg Gly Leu Glu Gly 
    290                 295                 300                 
Leu Val Ala Leu Pro Arg Gly Leu Tyr Thr Tyr Arg Gly Leu Ala Leu 
305                 310                 315                 320 
Ala Ala Ser Pro Ala Ser Pro Val Ala Leu Leu Glu Ala Leu Ala Ser 
                325                 330                 335     
Glu Arg Leu Glu Ala Leu Ala Leu Tyr Ser Leu Tyr Ser Ala Leu Ala 
            340                 345                 350         
Gly Leu Leu Tyr Ser Gly Leu Gly Leu Tyr Thr Tyr Arg Gly Leu Val 
        355                 360                 365             
Ala Leu Ala Arg Gly Ile Leu Glu Leu Glu Thr His Arg Ala Leu Ala 
    370                 375                 380                 
Ala Ser Pro Leu Tyr Ser Ala Ser Pro Leu Glu Thr Tyr Arg Gly Leu 
385                 390                 395                 400 
Asn Leu Glu Leu Glu Ser Glu Arg Ala Ser Pro Ala Arg Gly Ile Leu 
                405                 410                 415     
Glu His Ile Ser Val Ala Leu Leu Glu His Ile Ser Pro Arg Gly Leu 
            420                 425                 430         
Gly Leu Tyr Thr Tyr Arg Leu Glu Ile Leu Glu Thr His Arg Pro Arg 
        435                 440                 445             
Ala Leu Ala Thr Arg Pro Leu Glu Thr Arg Pro Gly Leu Leu Tyr Ser 
    450                 455                 460                 
Thr Tyr Arg Gly Leu Tyr Leu Glu Ala Arg Gly Pro Arg Ala Ser Pro 
465                 470                 475                 480 
Gly Leu Asn Thr Arg Pro Ala Leu Ala Ala Ser Pro Thr Tyr Arg Ala 
                485                 490                 495     
Arg Gly Ala Leu Ala Leu Glu Thr His Arg Gly Leu Tyr Ala Ser Pro 
            500                 505                 510         
Gly Leu Ser Glu Arg Ala Ser Pro Ala Ser Asn Leu Glu Pro Arg Gly 
        515                 520                 525             
Leu Tyr Val Ala Leu Leu Tyr Ser Gly Leu Tyr Ile Leu Glu Gly Leu 
    530                 535                 540                 
Tyr Gly Leu Leu Tyr Ser Thr His Arg Ala Leu Ala Ala Arg Gly Leu 
545                 550                 555                 560 
Tyr Ser Leu Glu Leu Glu Gly Leu Gly Leu Thr Arg Pro Gly Leu Tyr 
                565                 570                 575     
Ser Glu Arg Leu Glu Gly Leu Ala Leu Ala Leu Glu Leu Glu Leu Tyr 
            580                 585                 590         
Ser Ala Ser Asn Leu Glu Ala Ser Pro Ala Arg Gly Leu Glu Leu Tyr 
        595                 600                 605             
Ser Pro Arg Ala Leu Ala Ile Leu Glu Ala Arg Gly Gly Leu Leu Tyr 
    610                 615                 620                 
Ser Ile Leu Glu Leu Glu Ala Leu Ala His Ile Ser Met Glu Thr Ala 
625                 630                 635                 640 
Ser Pro Ala Ser Pro Leu Glu Leu Tyr Ser Leu Glu Ser Glu Arg Thr 
                645                 650                 655     
Arg Pro Ala Ser Pro Leu Glu Ala Leu Ala Leu Tyr Ser Val Ala Leu 
            660                 665                 670         
Ala Arg Gly Thr His Arg Ala Ser Pro Leu Glu Pro Arg Leu Glu Gly 
        675                 680                 685             
Leu Val Ala Leu Ala Ser Pro Pro His Glu Ala Leu Ala Leu Tyr Ser 
    690                 695                 700                 
Ala Arg Gly Ala Arg Gly Gly Leu Pro Arg Ala Ser Pro Ala Arg Gly 
705                 710                 715                 720 
Gly Leu Ala Arg Gly Leu Glu Ala Arg Gly Ala Leu Ala Pro His Glu 
                725                 730                 735     
Leu Glu Gly Leu Ala Arg Gly Leu Glu Gly Leu Pro His Glu Gly Leu 
            740                 745                 750         
Tyr Ser Glu Arg Leu Glu Leu Glu His Ile Ser Gly Leu Pro His Glu 
        755                 760                 765             
Gly Leu Tyr Leu Glu Leu Glu Gly Leu Ser Glu Arg Pro Arg Leu Tyr 
    770                 775                 780                 
Ser Ala Leu Ala Leu Glu Gly Leu Gly Leu Ala Leu Ala Pro Arg Thr 
785                 790                 795                 800 
Arg Pro Pro Arg Pro Arg Pro Arg Gly Leu Gly Leu Tyr Ala Leu Ala 
                805                 810                 815     
Pro His Glu Val Ala Leu Gly Leu Tyr Pro His Glu Val Ala Leu Leu 
            820                 825                 830         
Glu Ser Glu Arg Ala Arg Gly Leu Tyr Ser Gly Leu Pro Arg Met Glu 
        835                 840                 845             
Thr Thr Arg Pro Ala Leu Ala Ala Ser Pro Leu Glu Leu Glu Ala Leu 
    850                 855                 860                 
Ala Leu Glu Ala Leu Ala Ala Leu Ala Ala Leu Ala Ala Arg Gly Gly 
865                 870                 875                 880 
Leu Tyr Gly Leu Tyr Ala Arg Gly Val Ala Leu His Ile Ser Ala Arg 
                885                 890                 895     
Gly Ala Leu Ala Pro Arg Gly Leu Pro Arg Thr Tyr Arg Leu Tyr Ser 
            900                 905                 910         
Ala Leu Ala Leu Glu Ala Arg Gly Ala Ser Pro Leu Glu Leu Tyr Ser 
        915                 920                 925             
Gly Leu Ala Leu Ala Ala Arg Gly Gly Leu Tyr Leu Glu Leu Glu Ala 
    930                 935                 940                 
Leu Ala Leu Tyr Ser Ala Ser Pro Leu Glu Ser Glu Arg Val Ala Leu 
945                 950                 955                 960 
Leu Glu Ala Leu Ala Leu Glu Ala Arg Gly Gly Leu Gly Leu Tyr Leu 
                965                 970                 975     
Glu Gly Leu Tyr Leu Glu Pro Arg Pro Arg Gly Leu Tyr Ala Ser Pro 
            980                 985                 990         
Ala Ser Pro Pro Arg Met Glu Thr Leu Glu Leu Glu Ala Leu Ala Thr 
        995                 1000                1005            
Tyr Arg Leu Glu Leu Glu Ala Ser Pro Pro Arg Ser Glu Arg Ala Ser 
    1010                1015                1020                
Asn Thr His Arg Thr His Arg Pro Arg Gly Leu Gly Leu Tyr Val Ala 
1025                1030                1035                1040
Leu Ala Leu Ala Ala Arg Gly Ala Arg Gly Thr Tyr Arg Gly Leu Tyr 
                1045                1050                1055    
Gly Leu Tyr Gly Leu Thr Arg Pro Thr His Arg Gly Leu Gly Leu Ala 
            1060                1065                1070        
Leu Ala Gly Leu Tyr Gly Leu Ala Arg Gly Ala Leu Ala Ala Leu Ala 
        1075                1080                1085            
Leu Glu Ser Glu Arg Gly Leu Ala Arg Gly Leu Glu Pro His Glu Ala 
    1090                1095                1100                
Leu Ala Ala Ser Asn Leu Glu Thr Arg Pro Gly Leu Tyr Ala Arg Gly 
1105                1110                1115                1120
Leu Glu Gly Leu Gly Leu Tyr Gly Leu Gly Leu Ala Arg Gly Leu Glu 
                1125                1130                1135    
Leu Glu Thr Arg Pro Leu Glu Thr Tyr Arg Ala Arg Gly Gly Leu Val 
            1140                1145                1150        
Ala Leu Gly Leu Ala Arg Gly Pro Arg Leu Glu Ser Glu Arg Ala Leu 
        1155                1160                1165            
Ala Val Ala Leu Leu Glu Ala Leu Ala His Ile Ser Met Glu Thr Gly 
    1170                1175                1180                
Leu Ala Leu Ala Thr His Arg Gly Leu Tyr Val Ala Leu Ala Arg Gly 
1185                1190                1195                1200
Leu Glu Ala Ser Pro Val Ala Leu Ala Leu Ala Thr Tyr Arg Leu Glu 
                1205                1210                1215    
Ala Arg Gly Ala Leu Ala Leu Glu Ser Glu Arg Leu Glu Gly Leu Val 
            1220                1225                1230        
Ala Leu Ala Leu Ala Gly Leu Gly Leu Ile Leu Glu Ala Leu Ala Ala 
        1235                1240                1245            
Arg Gly Leu Glu Gly Leu Ala Leu Ala Gly Leu Val Ala Leu Pro His 
    1250                1255                1260                
Glu Ala Arg Gly Leu Glu Ala Leu Ala Gly Leu Tyr His Ile Ser Pro 
1265                1270                1275                1280
Arg Pro His Glu Ala Ser Asn Leu Glu Ala Ser Asn Ser Glu Arg Ala 
                1285                1290                1295    
Arg Gly Ala Ser Pro Gly Leu Asn Leu Glu Gly Leu Ala Arg Gly Val 
            1300                1305                1310        
Ala Leu Leu Glu Pro His Glu Ala Ser Pro Gly Leu Leu Glu Gly Leu 
        1315                1320                1325            
Tyr Leu Glu Pro Arg Ala Leu Ala Ile Leu Glu Gly Leu Tyr Leu Tyr 
    1330                1335                1340                
Ser Thr His Arg Gly Leu Leu Tyr Ser Thr His Arg Gly Leu Tyr Leu 
1345                1350                1355                1360
Tyr Ser Ala Arg Gly Ser Glu Arg Thr His Arg Ser Glu Arg Ala Leu 
                1365                1370                1375    
Ala Ala Leu Ala Val Ala Leu Leu Glu Gly Leu Ala Leu Ala Leu Glu 
            1380                1385                1390        
Ala Arg Gly Gly Leu Ala Leu Ala His Ile Ser Pro Arg Ile Leu Glu 
        1395                1400                1405            
Val Ala Leu Gly Leu Leu Tyr Ser Ile Leu Glu Leu Glu Gly Leu Asn 
    1410                1415                1420                
Thr Tyr Arg Ala Arg Gly Gly Leu Leu Glu Thr His Arg Leu Tyr Ser 
1425                1430                1435                1440
Leu Glu Leu Tyr Ser Ser Glu Arg Thr His Arg Thr Tyr Arg Ile Leu 
                1445                1450                1455    
Glu Ala Ser Pro Pro Arg Leu Glu Pro Arg Ala Ser Pro Leu Glu Ile 
            1460                1465                1470        
Leu Glu His Ile Ser Pro Arg Ala Arg Gly Thr His Arg Gly Leu Tyr 
        1475                1480                1485            
Ala Arg Gly Leu Glu His Ile Ser Thr His Arg Ala Arg Gly Pro His 
    1490                1495                1500                
Glu Ala Ser Asn Gly Leu Asn Thr His Arg Ala Leu Ala Thr His Arg 
1505                1510                1515                1520
Ala Leu Ala Thr His Arg Gly Leu Tyr Ala Arg Gly Leu Glu Ser Glu 
                1525                1530                1535    
Arg Ser Glu Arg Ser Glu Arg Ala Ser Pro Pro Arg Ala Ser Asn Leu 
            1540                1545                1550        
Glu Gly Leu Asn Ala Ser Asn Ile Leu Glu Pro Arg Val Ala Leu Ala 
        1555                1560                1565            
Arg Gly Thr His Arg Pro Arg Leu Glu Gly Leu Tyr Gly Leu Asn Ala 
    1570                1575                1580                
Arg Gly Ile Leu Glu Ala Arg Gly Ala Arg Gly Ala Leu Ala Pro His 
1585                1590                1595                1600
Glu Ile Leu Glu Ala Leu Ala Gly Leu Gly Leu Gly Leu Tyr Thr Arg 
                1605                1610                1615    
Pro Leu Glu Leu Glu Val Ala Leu Ala Leu Ala Leu Glu Ala Ser Pro 
            1620                1625                1630        
Thr Tyr Arg Ser Glu Arg Gly Leu Asn Ile Leu Glu Gly Leu Leu Glu 
        1635                1640                1645            
Ala Arg Gly Val Ala Leu Leu Glu Ala Leu Ala His Ile Ser Ile Leu 
    1650                1655                1660                
Glu Ala Leu Ala Ala Ser Pro Ala Ser Pro Ala Ser Pro Ala Ser Asn 
1665                1670                1675                1680
Leu Glu Ile Leu Glu Gly Leu Ala Leu Ala Pro His Glu Gly Leu Asn 
                1685                1690                1695    
Ala Arg Gly Ala Ser Pro Leu Glu Ala Ser Pro Ile Leu Glu His Ile 
            1700                1705                1710        
Ser Thr His Arg Leu Tyr Ser Thr His Arg Ala Leu Ala Met Glu Thr 
        1715                1720                1725            
Ala Ser Pro Ile Leu Glu Pro His Glu His Ile Ser Val Ala Leu Ser 
    1730                1735                1740                
Glu Arg Gly Leu Gly Leu Gly Leu Val Ala Leu Thr His Arg Ala Leu 
1745                1750                1755                1760
Ala Ala Ser Asn Met Glu Thr Ala Arg Gly Ala Arg Gly Gly Leu Asn 
                1765                1770                1775    
Ala Leu Ala Leu Tyr Ser Ala Leu Ala Val Ala Leu Ala Ser Asn Thr 
            1780                1785                1790        
Tyr Arg Gly Leu Tyr Ile Leu Glu Val Ala Leu Thr Tyr Arg Gly Leu 
        1795                1800                1805            
Tyr Ile Leu Glu Ser Glu Arg Ala Ser Pro Thr Tyr Arg Gly Leu Tyr 
    1810                1815                1820                
Leu Glu Ala Leu Ala Gly Leu Asn Ala Ser Asn Leu Glu Ala Ser Asn 
1825                1830                1835                1840
Ile Leu Glu Thr His Arg Ala Arg Gly Leu Tyr Ser Gly Leu Ala Leu 
                1845                1850                1855    
Ala Ala Leu Ala Gly Leu Pro His Glu Ile Leu Glu Gly Leu Ala Arg 
            1860                1865                1870        
Gly Thr Tyr Arg Pro His Glu Gly Leu Asn Ser Glu Arg Pro His Glu 
        1875                1880                1885            
Pro Arg Leu Tyr Ser Val Ala Leu Ala Arg Gly Ala Leu Ala Thr Arg 
    1890                1895                1900                
Pro Ile Leu Glu Gly Leu Leu Tyr Ser Thr His Arg Leu Glu Gly Leu 
1905                1910                1915                1920
Gly Leu Gly Leu Tyr Ala Arg Gly Ala Arg Gly Ala Arg Gly Gly Leu 
                1925                1930                1935    
Tyr Thr Tyr Arg Val Ala Leu Gly Leu Thr His Arg Leu Glu Pro His 
            1940                1945                1950        
Glu Gly Leu Tyr Ala Arg Gly Ala Arg Gly Ala Arg Gly Thr Tyr Arg 
        1955                1960                1965            
Val Ala Leu Pro Arg Ala Ser Pro Leu Glu Gly Leu Ala Leu Ala Ala 
    1970                1975                1980                
Arg Gly Ala Ser Asn Pro His Glu Ala Ser Asn Val Ala Leu Ala Arg 
1985                1990                1995                2000
Gly Ser Glu Arg Pro His Glu Ala Leu Ala Gly Leu Ala Arg Gly Thr 
                2005                2010                2015    
His Arg Ala Leu Ala Met Glu Thr Ala Ser Asn Thr His Arg Pro Arg 
            2020                2025                2030        
Val Ala Leu Gly Leu Asn Gly Leu Tyr Thr His Arg Ala Leu Ala Ala 
        2035                2040                2045            
Leu Ala Ala Ser Pro Leu Glu Met Glu Thr Leu Tyr Ser Leu Glu Ala 
    2050                2055                2060                
Leu Ala Met Glu Thr Val Ala Leu Leu Tyr Ser Leu Glu Pro His Glu 
2065                2070                2075                2080
Pro Arg Ala Arg Gly Leu Glu Gly Leu Gly Leu Met Glu Thr Gly Leu 
                2085                2090                2095    
Tyr Ala Leu Ala Ala Arg Gly Met Glu Thr Leu Glu Leu Glu Gly Leu 
            2100                2105                2110        
Asn Val Ala Leu His Ile Ser Ala Ser Pro Gly Leu Leu Glu Val Ala 
        2115                2120                2125            
Leu Leu Glu Gly Leu Ala Leu Ala Pro Arg Leu Tyr Ser Gly Leu Ala 
    2130                2135                2140                
Arg Gly Ala Leu Ala Gly Leu Ala Leu Ala Val Ala Leu Ala Leu Ala 
2145                2150                2155                2160
Ala Arg Gly Leu Glu Ala Leu Ala Leu Tyr Ser Gly Leu Val Ala Leu 
                2165                2170                2175    
Met Glu Thr Gly Leu Gly Leu Tyr Val Ala Leu Thr Tyr Arg Pro Arg 
            2180                2185                2190        
Leu Glu Ala Leu Ala Val Ala Leu Pro Arg Leu Glu Gly Leu Val Ala 
        2195                2200                2205            
Leu Gly Leu Val Ala Leu Gly Leu Tyr Ile Leu Glu Gly Leu Tyr Gly 
    2210                2215                2220                
Leu Ala Ser Pro Thr Arg Pro Leu Glu Ser Glu Arg Ala Leu Ala Leu 
2225                2230                2235                2240
Tyr Ser Gly Leu 
                

<210> 3
<211> 834
<212> PRT
<213> Artificial Sequence

<220> 
<223> Amino acid sequence of wild-type Thermus thermophiles (Tth) DNA
      polymerase (SEQ ID NO: 3)

<400> 3
Met Glu Ala Met Leu Pro Leu Phe Glu Pro Lys Gly Arg Val Leu Leu 
1               5                   10                  15      
Val Asp Gly His His Leu Ala Tyr Arg Thr Phe Phe Ala Leu Lys Gly 
            20                  25                  30          
Leu Thr Thr Ser Arg Gly Glu Pro Val Gln Ala Val Tyr Gly Phe Ala 
        35                  40                  45              
Lys Ser Leu Leu Lys Ala Leu Lys Glu Asp Gly Tyr Lys Ala Val Phe 
    50                  55                  60                  
Val Val Phe Asp Ala Lys Ala Pro Ser Phe Arg His Glu Ala Tyr Glu 
65                  70                  75                  80  
Ala Tyr Lys Ala Gly Arg Ala Pro Thr Pro Glu Asp Phe Pro Arg Gln 
                85                  90                  95      
Leu Ala Leu Ile Lys Glu Leu Val Asp Leu Leu Gly Phe Thr Arg Leu 
            100                 105                 110         
Glu Val Pro Gly Tyr Glu Ala Asp Asp Val Leu Ala Thr Leu Ala Lys 
        115                 120                 125             
Lys Ala Glu Lys Glu Gly Tyr Glu Val Arg Ile Leu Thr Ala Asp Arg 
    130                 135                 140                 
Asp Leu Tyr Gln Leu Val Ser Asp Arg Val Ala Val Leu His Pro Glu 
145                 150                 155                 160 
Gly His Leu Ile Thr Pro Glu Trp Leu Trp Glu Lys Tyr Gly Leu Arg 
                165                 170                 175     
Pro Glu Gln Trp Val Asp Phe Arg Ala Leu Val Gly Asp Pro Ser Asp 
            180                 185                 190         
Asn Leu Pro Gly Val Lys Gly Ile Gly Glu Lys Thr Ala Leu Lys Leu 
        195                 200                 205             
Leu Lys Glu Trp Gly Ser Leu Glu Asn Leu Leu Lys Asn Leu Asp Arg 
    210                 215                 220                 
Val Lys Pro Glu Asn Val Arg Glu Lys Ile Lys Ala His Leu Glu Asp 
225                 230                 235                 240 
Leu Arg Leu Ser Leu Glu Leu Ser Arg Val Arg Thr Asp Leu Pro Leu 
                245                 250                 255     
Glu Val Asp Leu Ala Gln Gly Arg Glu Pro Asp Arg Glu Gly Leu Arg 
            260                 265                 270         
Ala Phe Leu Glu Arg Leu Glu Phe Gly Ser Leu Leu His Glu Phe Gly 
        275                 280                 285             
Leu Leu Glu Ala Pro Ala Pro Leu Glu Glu Ala Pro Trp Pro Pro Pro 
    290                 295                 300                 
Glu Gly Ala Phe Val Gly Phe Val Leu Ser Arg Pro Glu Pro Met Trp 
305                 310                 315                 320 
Ala Glu Leu Lys Ala Leu Ala Ala Cys Arg Asp Gly Arg Val His Arg 
                325                 330                 335     
Ala Ala Asp Pro Leu Ala Gly Leu Lys Asp Leu Lys Glu Val Arg Gly 
            340                 345                 350         
Leu Leu Ala Lys Asp Leu Ala Val Leu Ala Ser Arg Glu Gly Leu Asp 
        355                 360                 365             
Leu Val Pro Gly Asp Asp Pro Met Leu Leu Ala Tyr Leu Leu Asp Pro 
    370                 375                 380                 
Ser Asn Thr Thr Pro Glu Gly Val Ala Arg Arg Tyr Gly Gly Glu Trp 
385                 390                 395                 400 
Thr Glu Asp Ala Ala His Arg Ala Leu Leu Ser Glu Arg Leu His Arg 
                405                 410                 415     
Asn Leu Leu Lys Arg Leu Glu Gly Glu Glu Lys Leu Leu Trp Leu Tyr 
            420                 425                 430         
His Glu Val Glu Lys Pro Leu Ser Arg Val Leu Ala His Met Glu Ala 
        435                 440                 445             
Thr Gly Val Arg Arg Asp Val Ala Tyr Leu Gln Ala Leu Ser Leu Glu 
    450                 455                 460                 
Leu Ala Glu Glu Ile Arg Arg Leu Glu Glu Glu Val Phe Arg Leu Ala 
465                 470                 475                 480 
Gly His Pro Phe Asn Leu Asn Ser Arg Asp Gln Leu Glu Arg Val Leu 
                485                 490                 495     
Phe Asp Glu Leu Arg Leu Pro Ala Leu Gly Lys Thr Gln Lys Thr Gly 
            500                 505                 510         
Lys Arg Ser Thr Ser Ala Ala Val Leu Glu Ala Leu Arg Glu Ala His 
        515                 520                 525             
Pro Ile Val Glu Lys Ile Leu Gln His Arg Glu Leu Thr Lys Leu Lys 
    530                 535                 540                 
Asn Thr Tyr Val Asp Pro Leu Pro Ser Leu Val His Pro Arg Thr Gly 
545                 550                 555                 560 
Arg Leu His Thr Arg Phe Asn Gln Thr Ala Thr Ala Thr Gly Arg Leu 
                565                 570                 575     
Ser Ser Ser Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Thr Pro Leu 
            580                 585                 590         
Gly Gln Arg Ile Arg Arg Ala Phe Val Ala Glu Ala Gly Trp Ala Leu 
        595                 600                 605             
Val Ala Leu Asp Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Leu 
    610                 615                 620                 
Ser Gly Asp Glu Asn Leu Ile Arg Val Phe Gln Glu Gly Lys Asp Ile 
625                 630                 635                 640 
His Thr Gln Thr Ala Ser Trp Met Phe Gly Val Pro Pro Glu Ala Val 
                645                 650                 655     
Asp Pro Leu Met Arg Arg Ala Ala Lys Thr Val Asn Phe Gly Val Leu 
            660                 665                 670         
Tyr Gly Met Ser Ala His Arg Leu Ser Gln Glu Leu Ala Ile Pro Tyr 
        675                 680                 685             
Glu Glu Ala Val Ala Phe Ile Glu Arg Tyr Phe Gln Ser Phe Pro Lys 
    690                 695                 700                 
Val Arg Ala Trp Ile Glu Lys Thr Leu Glu Glu Gly Arg Lys Arg Gly 
705                 710                 715                 720 
Tyr Val Glu Thr Leu Phe Gly Arg Arg Arg Tyr Val Pro Asp Leu Asn 
                725                 730                 735     
Ala Arg Val Lys Ser Val Arg Glu Ala Ala Glu Arg Met Ala Phe Asn 
            740                 745                 750         
Met Pro Val Gln Gly Thr Ala Ala Asp Leu Met Lys Leu Ala Met Val 
        755                 760                 765             
Lys Leu Phe Pro Arg Leu Arg Glu Met Gly Ala Arg Met Leu Leu Gln 
    770                 775                 780                 
Val His Asp Glu Leu Leu Leu Glu Ala Pro Gln Ala Arg Ala Glu Glu 
785                 790                 795                 800 
Val Ala Ala Leu Ala Lys Glu Ala Met Glu Lys Ala Tyr Pro Leu Ala 
                805                 810                 815     
Val Pro Leu Glu Val Glu Val Gly Met Gly Glu Asp Trp Leu Ser Ala 
            820                 825                 830         
Lys Gly 
        

<210> 4
<211> 834
<212> PRT
<213> Artificial Sequence

<220> 
<223> Amino acid sequence of a chimeric DNA-Polymerase variant (SEQ ID
      NO:4), derived from a parent Tth DNA-Polymerase and Bst
      Polymerase I.

<400> 4
Met Glu Ala Met Leu Pro Leu Phe Glu Pro Lys Gly Arg Val Leu Leu 
1               5                   10                  15      
Val Asp Gly His His Leu Ala Tyr Arg Thr Phe Phe Ala Leu Lys Gly 
            20                  25                  30          
Leu Thr Thr Ser Arg Gly Glu Pro Val Gln Ala Val Tyr Asp Phe Ala 
        35                  40                  45              
Lys Ser Leu Leu Lys Ala Leu Lys Glu Asp Gly Tyr Lys Ala Val Phe 
    50                  55                  60                  
Val Val Phe Asp Ala Lys Ala Pro Ser Phe Arg His Glu Ala Tyr Glu 
65                  70                  75                  80  
Ala Tyr Lys Ala Gly Arg Ala Pro Thr Pro Glu Asp Phe Pro Arg Gln 
                85                  90                  95      
Leu Ala Leu Ile Lys Glu Leu Val Asp Leu Leu Gly Phe Thr Arg Leu 
            100                 105                 110         
Glu Val Pro Gly Tyr Glu Ala Asp Asp Val Leu Ala Thr Leu Ala Lys 
        115                 120                 125             
Lys Ala Glu Lys Glu Gly Tyr Glu Val Arg Ile Leu Thr Ala Asp Arg 
    130                 135                 140                 
Asp Leu Tyr Gln Leu Val Ser Asp Arg Val Ala Val Leu His Pro Glu 
145                 150                 155                 160 
Gly His Leu Ile Thr Pro Glu Trp Leu Trp Glu Lys Tyr Gly Leu Arg 
                165                 170                 175     
Pro Glu Gln Trp Val Asp Phe Arg Ala Leu Val Gly Asp Pro Ser Asp 
            180                 185                 190         
Asn Leu Pro Gly Val Lys Gly Ile Gly Glu Lys Thr Ala Leu Lys Leu 
        195                 200                 205             
Leu Lys Glu Trp Gly Ser Leu Glu Asn Leu Leu Lys Asn Leu Asp Arg 
    210                 215                 220                 
Val Lys Pro Glu Asn Val Arg Glu Lys Ile Lys Ala His Leu Glu Asp 
225                 230                 235                 240 
Leu Arg Leu Ser Leu Glu Leu Ser Arg Val Arg Thr Asp Leu Pro Leu 
                245                 250                 255     
Glu Val Asp Leu Ala Gln Gly Arg Glu Pro Asp Arg Glu Gly Leu Arg 
            260                 265                 270         
Ala Phe Leu Glu Arg Leu Glu Phe Gly Ser Leu Leu His Glu Phe Gly 
        275                 280                 285             
Leu Leu Glu Ala Pro Ala Pro Leu Glu Glu Ala Pro Trp Pro Pro Pro 
    290                 295                 300                 
Glu Gly Ala Phe Val Gly Phe Val Leu Ser Arg Pro Glu Pro Met Trp 
305                 310                 315                 320 
Ala Glu Leu Lys Ala Leu Ala Ala Cys Arg Asp Gly Arg Val His Arg 
                325                 330                 335     
Ala Ala Asp Pro Leu Ala Gly Leu Lys Asp Leu Lys Glu Val Arg Gly 
            340                 345                 350         
Leu Leu Ala Lys Asp Leu Ala Val Leu Ala Ser Arg Glu Gly Leu Asp 
        355                 360                 365             
Leu Val Pro Gly Asp Asp Pro Met Leu Leu Ala Tyr Leu Leu Asp Pro 
    370                 375                 380                 
Ser Asn Thr Thr Pro Glu Gly Val Ala Arg Arg Tyr Gly Gly Glu Trp 
385                 390                 395                 400 
Thr Glu Asp Ala Ala His Arg Ala Leu Leu Ser Glu Arg Leu His Arg 
                405                 410                 415     
Asn Leu Leu Lys Arg Leu Glu Gly Glu Glu Lys Leu Leu Trp Leu Tyr 
            420                 425                 430         
His Glu Val Glu Lys Pro Leu Ser Arg Val Leu Ala His Met Glu Ala 
        435                 440                 445             
Thr Gly Val Arg Arg Asp Val Ala Tyr Leu Gln Ala Leu Ser Leu Glu 
    450                 455                 460                 
Leu Ala Glu Glu Ile Arg Arg Leu Glu Glu Glu Val Phe Arg Leu Ala 
465                 470                 475                 480 
Gly His Pro Phe Asn Leu Asn Ser Arg Asp Gln Leu Glu Arg Val Leu 
                485                 490                 495     
Phe Asp Glu Leu Arg Leu Pro Ala Leu Gly Lys Thr Gln Lys Thr Gly 
            500                 505                 510         
Lys Arg Ser Thr Ser Ala Ala Val Leu Glu Ala Leu Arg Glu Ala His 
        515                 520                 525             
Pro Ile Val Glu Lys Ile Leu Gln His Arg Glu Leu Thr Lys Leu Lys 
    530                 535                 540                 
Asn Thr Tyr Val Asp Pro Leu Pro Ser Leu Val His Pro Arg Thr Gly 
545                 550                 555                 560 
Arg Leu His Thr Arg Phe Asn Gln Thr Ala Thr Ala Thr Gly Arg Leu 
                565                 570                 575     
Ser Ser Ser Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Thr Pro Leu 
            580                 585                 590         
Gly Gln Arg Ile Arg Arg Ala Phe Val Ala Glu Ala Gly Trp Ala Leu 
        595                 600                 605             
Val Ala Leu Asp Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Ile 
    610                 615                 620                 
Ala Asp Asp Asp Asn Leu Ile Glu Ala Phe Gln Arg Asp Leu Asp Ile 
625                 630                 635                 640 
His Thr Lys Thr Ala Met Asp Ile Phe His Val Ser Glu Glu Glu Val 
                645                 650                 655     
Thr Ala Asn Met Arg Arg Gln Ala Lys Ala Val Asn Phe Gly Ile Val 
            660                 665                 670         
Tyr Gly Ile Ser Asp Tyr Gly Leu Ala Gln Asn Leu Asn Ile Thr Arg 
        675                 680                 685             
Lys Glu Ala Ala Glu Phe Ile Glu Arg Tyr Phe Gln Ser Phe Pro Lys 
    690                 695                 700                 
Val Arg Ala Trp Ile Glu Lys Thr Leu Glu Glu Gly Arg Lys Arg Gly 
705                 710                 715                 720 
Tyr Val Glu Thr Leu Phe Gly Arg Arg Arg Tyr Val Pro Asp Leu Asn 
                725                 730                 735     
Ala Arg Asn Phe Asn Val Arg Ser Phe Ala Glu Arg Thr Ala Met Asn 
            740                 745                 750         
Thr Pro Val Gln Gly Thr Ala Ala Asp Leu Met Lys Leu Ala Met Val 
        755                 760                 765             
Lys Leu Phe Pro Arg Leu Arg Glu Met Gly Ala Arg Met Leu Leu Gln 
    770                 775                 780                 
Val His Asp Glu Leu Leu Leu Glu Ala Pro Gln Ala Arg Ala Glu Glu 
785                 790                 795                 800 
Val Ala Ala Leu Ala Lys Glu Ala Met Glu Lys Ala Tyr Pro Leu Ala 
                805                 810                 815     
Val Pro Leu Glu Val Glu Val Gly Met Gly Glu Asp Trp Leu Ser Ala 
            820                 825                 830         
Lys Gly 
        

<210> 5
<211> 831
<212> PRT
<213> Artificial Sequence

<220> 
<223> Amino acid sequence of wild-type Thermus Flavus (Tfl) DNA
      polymerase (SEQ ID NO: 5)

<400> 5
Met Ala Met Leu Pro Leu Phe Glu Pro Lys Gly Arg Val Leu Leu Val 
1               5                   10                  15      
Asp Gly His His Leu Ala Tyr Arg Thr Phe Phe Ala Leu Lys Gly Leu 
            20                  25                  30          
Thr Thr Ser Arg Gly Glu Pro Val Gln Ala Val Tyr Gly Phe Ala Lys 
        35                  40                  45              
Ser Leu Leu Lys Ala Leu Lys Glu Asp Gly Asp Val Val Val Val Val 
    50                  55                  60                  
Phe Asp Ala Lys Ala Pro Ser Phe Arg His Glu Ala Tyr Glu Ala Tyr 
65                  70                  75                  80  
Lys Ala Gly Arg Ala Pro Thr Pro Glu Asp Phe Pro Arg Gln Leu Ala 
                85                  90                  95      
Leu Ile Lys Glu Leu Val Asp Leu Leu Gly Leu Val Arg Leu Glu Val 
            100                 105                 110         
Pro Gly Phe Glu Ala Asp Asp Val Leu Ala Thr Leu Ala Lys Arg Ala 
        115                 120                 125             
Glu Lys Glu Gly Tyr Glu Val Arg Ile Leu Thr Ala Asp Arg Asp Leu 
    130                 135                 140                 
Tyr Gln Leu Leu Ser Glu Arg Ile Ala Ile Leu His Pro Glu Gly Tyr 
145                 150                 155                 160 
Leu Ile Thr Pro Ala Trp Leu Tyr Glu Lys Tyr Gly Leu Arg Pro Glu 
                165                 170                 175     
Gln Trp Val Asp Tyr Arg Ala Leu Ala Gly Asp Pro Ser Asp Asn Ile 
            180                 185                 190         
Pro Gly Val Lys Gly Ile Gly Glu Lys Thr Ala Gln Arg Leu Ile Arg 
        195                 200                 205             
Glu Trp Gly Ser Leu Glu Asn Leu Phe Gln His Leu Asp Gln Val Lys 
    210                 215                 220                 
Pro Ser Leu Arg Glu Lys Leu Gln Ala Gly Met Glu Ala Leu Ala Leu 
225                 230                 235                 240 
Ser Arg Lys Leu Ser Gln Val His Thr Asp Leu Pro Leu Glu Val Asp 
                245                 250                 255     
Phe Gly Arg Arg Arg Thr Pro Asn Leu Glu Gly Leu Arg Ala Phe Leu 
            260                 265                 270         
Glu Arg Leu Glu Phe Gly Ser Leu Leu His Glu Phe Gly Leu Leu Glu 
        275                 280                 285             
Gly Pro Lys Ala Ala Glu Glu Ala Pro Trp Pro Pro Pro Glu Gly Ala 
    290                 295                 300                 
Phe Leu Gly Phe Ser Phe Ser Arg Pro Glu Pro Met Trp Ala Glu Leu 
305                 310                 315                 320 
Leu Ala Leu Ala Gly Ala Trp Glu Gly Arg Leu His Arg Ala Gln Asp 
                325                 330                 335     
Pro Leu Arg Gly Leu Arg Asp Leu Lys Gly Val Arg Gly Ile Leu Ala 
            340                 345                 350         
Lys Asp Leu Ala Val Leu Ala Leu Arg Glu Gly Leu Asp Leu Phe Pro 
        355                 360                 365             
Glu Asp Asp Pro Met Leu Leu Ala Tyr Leu Leu Asp Pro Ser Asn Thr 
    370                 375                 380                 
Thr Pro Glu Gly Val Ala Arg Arg Tyr Gly Gly Glu Trp Thr Glu Asp 
385                 390                 395                 400 
Ala Gly Glu Arg Ala Leu Leu Ala Glu Arg Leu Phe Gln Thr Leu Lys 
                405                 410                 415     
Glu Arg Leu Lys Gly Glu Glu Arg Leu Leu Trp Leu Tyr Glu Glu Val 
            420                 425                 430         
Glu Lys Pro Leu Ser Arg Val Leu Ala Arg Met Glu Ala Thr Gly Val 
        435                 440                 445             
Arg Leu Asp Val Ala Tyr Leu Gln Ala Leu Ser Leu Glu Val Glu Ala 
    450                 455                 460                 
Glu Val Arg Gln Leu Glu Glu Glu Val Phe Arg Leu Ala Gly His Pro 
465                 470                 475                 480 
Phe Asn Leu Asn Ser Arg Asp Gln Leu Glu Arg Val Leu Phe Asp Glu 
                485                 490                 495     
Leu Gly Leu Pro Ala Ile Gly Lys Thr Glu Lys Thr Gly Lys Arg Ser 
            500                 505                 510         
Thr Ser Ala Ala Val Leu Glu Ala Leu Arg Glu Ala His Pro Ile Val 
        515                 520                 525             
Asp Arg Ile Leu Gln Tyr Arg Glu Leu Thr Lys Leu Lys Asn Thr Tyr 
    530                 535                 540                 
Ile Asp Pro Leu Pro Ala Leu Val His Pro Lys Thr Gly Arg Leu His 
545                 550                 555                 560 
Thr Arg Phe Asn Gln Thr Ala Thr Ala Thr Gly Arg Leu Ser Ser Ser 
                565                 570                 575     
Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Thr Pro Leu Gly Gln Arg 
            580                 585                 590         
Ile Arg Arg Ala Phe Val Ala Glu Glu Gly Trp Val Leu Val Val Leu 
        595                 600                 605             
Asp Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Leu Ser Gly Asp 
    610                 615                 620                 
Glu Asn Leu Ile Arg Val Phe Gln Glu Gly Arg Asp Ile His Thr Gln 
625                 630                 635                 640 
Thr Ala Ser Trp Met Phe Gly Val Ser Pro Glu Gly Val Asp Pro Leu 
                645                 650                 655     
Met Arg Arg Ala Ala Lys Thr Ile Asn Phe Gly Val Leu Tyr Gly Met 
            660                 665                 670         
Ser Ala His Arg Leu Ser Gly Glu Leu Ser Ile Pro Tyr Glu Glu Ala 
        675                 680                 685             
Val Ala Phe Ile Glu Arg Tyr Phe Gln Ser Tyr Pro Lys Val Arg Ala 
    690                 695                 700                 
Trp Ile Glu Gly Thr Leu Glu Glu Gly Arg Arg Arg Gly Tyr Val Glu 
705                 710                 715                 720 
Thr Leu Phe Gly Arg Arg Arg Tyr Val Pro Asp Leu Asn Ala Arg Val 
                725                 730                 735     
Lys Ser Val Arg Glu Ala Ala Glu Arg Met Ala Phe Asn Met Pro Val 
            740                 745                 750         
Gln Gly Thr Ala Ala Asp Leu Met Lys Leu Ala Met Val Arg Leu Phe 
        755                 760                 765             
Pro Arg Leu Gln Glu Leu Gly Ala Arg Met Leu Leu Gln Val His Asp 
    770                 775                 780                 
Glu Leu Val Leu Glu Ala Pro Lys Asp Arg Ala Glu Arg Val Ala Ala 
785                 790                 795                 800 
Leu Ala Lys Glu Val Met Glu Gly Val Trp Pro Leu Gln Val Pro Leu 
                805                 810                 815     
Glu Val Glu Val Gly Leu Gly Glu Asp Trp Leu Ser Ala Lys Glu 
            820                 825                 830     

<210> 6
<211> 831
<212> PRT
<213> Artificial Sequence

<220> 
<223> Amino acid sequence of a chimeric DNA-Polymerase variant (SEQ ID
      NO:6), derived from a parent Tfl DNA-Polymerase and Bst
      Polymerase I

<400> 6
Met Ala Met Leu Pro Leu Phe Glu Pro Lys Gly Arg Val Leu Leu Val 
1               5                   10                  15      
Asp Gly His His Leu Ala Tyr Arg Thr Phe Phe Ala Leu Lys Gly Leu 
            20                  25                  30          
Thr Thr Ser Arg Gly Glu Pro Val Gln Ala Val Tyr Asp Phe Ala Lys 
        35                  40                  45              
Ser Leu Leu Lys Ala Leu Lys Glu Asp Gly Asp Val Val Val Val Val 
    50                  55                  60                  
Phe Asp Ala Lys Ala Pro Ser Phe Arg His Glu Ala Tyr Glu Ala Tyr 
65                  70                  75                  80  
Lys Ala Gly Arg Ala Pro Thr Pro Glu Asp Phe Pro Arg Gln Leu Ala 
                85                  90                  95      
Leu Ile Lys Glu Leu Val Asp Leu Leu Gly Leu Val Arg Leu Glu Val 
            100                 105                 110         
Pro Gly Phe Glu Ala Asp Asp Val Leu Ala Thr Leu Ala Lys Arg Ala 
        115                 120                 125             
Glu Lys Glu Gly Tyr Glu Val Arg Ile Leu Thr Ala Asp Arg Asp Leu 
    130                 135                 140                 
Tyr Gln Leu Leu Ser Glu Arg Ile Ala Ile Leu His Pro Glu Gly Tyr 
145                 150                 155                 160 
Leu Ile Thr Pro Ala Trp Leu Tyr Glu Lys Tyr Gly Leu Arg Pro Glu 
                165                 170                 175     
Gln Trp Val Asp Tyr Arg Ala Leu Ala Gly Asp Pro Ser Asp Asn Ile 
            180                 185                 190         
Pro Gly Val Lys Gly Ile Gly Glu Lys Thr Ala Gln Arg Leu Ile Arg 
        195                 200                 205             
Glu Trp Gly Ser Leu Glu Asn Leu Phe Gln His Leu Asp Gln Val Lys 
    210                 215                 220                 
Pro Ser Leu Arg Glu Lys Leu Gln Ala Gly Met Glu Ala Leu Ala Leu 
225                 230                 235                 240 
Ser Arg Lys Leu Ser Gln Val His Thr Asp Leu Pro Leu Glu Val Asp 
                245                 250                 255     
Phe Gly Arg Arg Arg Thr Pro Asn Leu Glu Gly Leu Arg Ala Phe Leu 
            260                 265                 270         
Glu Arg Leu Glu Phe Gly Ser Leu Leu His Glu Phe Gly Leu Leu Glu 
        275                 280                 285             
Gly Pro Lys Ala Ala Glu Glu Ala Pro Trp Pro Pro Pro Glu Gly Ala 
    290                 295                 300                 
Phe Leu Gly Phe Ser Phe Ser Arg Pro Glu Pro Met Trp Ala Glu Leu 
305                 310                 315                 320 
Leu Ala Leu Ala Gly Ala Trp Glu Gly Arg Leu His Arg Ala Gln Asp 
                325                 330                 335     
Pro Leu Arg Gly Leu Arg Asp Leu Lys Gly Val Arg Gly Ile Leu Ala 
            340                 345                 350         
Lys Asp Leu Ala Val Leu Ala Leu Arg Glu Gly Leu Asp Leu Phe Pro 
        355                 360                 365             
Glu Asp Asp Pro Met Leu Leu Ala Tyr Leu Leu Asp Pro Ser Asn Thr 
    370                 375                 380                 
Thr Pro Glu Gly Val Ala Arg Arg Tyr Gly Gly Glu Trp Thr Glu Asp 
385                 390                 395                 400 
Ala Gly Glu Arg Ala Leu Leu Ala Glu Arg Leu Phe Gln Thr Leu Lys 
                405                 410                 415     
Glu Arg Leu Lys Gly Glu Glu Arg Leu Leu Trp Leu Tyr Glu Glu Val 
            420                 425                 430         
Glu Lys Pro Leu Ser Arg Val Leu Ala Arg Met Glu Ala Thr Gly Val 
        435                 440                 445             
Arg Leu Asp Val Ala Tyr Leu Gln Ala Leu Ser Leu Glu Val Glu Ala 
    450                 455                 460                 
Glu Val Arg Gln Leu Glu Glu Glu Val Phe Arg Leu Ala Gly His Pro 
465                 470                 475                 480 
Phe Asn Leu Asn Ser Arg Asp Gln Leu Glu Arg Val Leu Phe Asp Glu 
                485                 490                 495     
Leu Gly Leu Pro Ala Ile Gly Lys Thr Glu Lys Thr Gly Lys Arg Ser 
            500                 505                 510         
Thr Ser Ala Ala Val Leu Glu Ala Leu Arg Glu Ala His Pro Ile Val 
        515                 520                 525             
Asp Arg Ile Leu Gln Tyr Arg Glu Leu Thr Lys Leu Lys Asn Thr Tyr 
    530                 535                 540                 
Ile Asp Pro Leu Pro Ala Leu Val His Pro Lys Thr Gly Arg Leu His 
545                 550                 555                 560 
Thr Arg Phe Asn Gln Thr Ala Thr Ala Thr Gly Arg Leu Ser Ser Ser 
                565                 570                 575     
Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Thr Pro Leu Gly Gln Arg 
            580                 585                 590         
Ile Arg Arg Ala Phe Val Ala Glu Glu Gly Trp Val Leu Val Val Leu 
        595                 600                 605             
Asp Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Ile Ala Asp Asp 
    610                 615                 620                 
Asp Asn Leu Ile Glu Ala Phe Gln Arg Asp Leu Asp Ile His Thr Lys 
625                 630                 635                 640 
Thr Ala Met Asp Ile Phe His Val Ser Glu Glu Glu Val Thr Ala Asn 
                645                 650                 655     
Met Arg Arg Gln Ala Lys Ala Val Asn Phe Gly Ile Val Tyr Gly Ile 
            660                 665                 670         
Ser Asp Tyr Gly Leu Ala Gln Asn Leu Asn Ile Thr Arg Lys Glu Ala 
        675                 680                 685             
Ala Glu Phe Ile Glu Arg Tyr Phe Gln Ser Tyr Pro Lys Val Arg Ala 
    690                 695                 700                 
Trp Ile Glu Gly Thr Leu Glu Glu Gly Arg Arg Arg Gly Tyr Val Glu 
705                 710                 715                 720 
Thr Leu Phe Gly Arg Arg Arg Tyr Val Pro Asp Leu Asn Ala Arg Asn 
                725                 730                 735     
Phe Asn Val Arg Ser Phe Ala Glu Arg Thr Ala Met Asn Thr Pro Val 
            740                 745                 750         
Gln Gly Thr Ala Ala Asp Leu Met Lys Leu Ala Met Val Arg Leu Phe 
        755                 760                 765             
Pro Arg Leu Gln Glu Leu Gly Ala Arg Met Leu Leu Gln Val His Asp 
    770                 775                 780                 
Glu Leu Val Leu Glu Ala Pro Lys Asp Arg Ala Glu Arg Val Ala Ala 
785                 790                 795                 800 
Leu Ala Lys Glu Val Met Glu Gly Val Trp Pro Leu Gln Val Pro Leu 
                805                 810                 815     
Glu Val Glu Val Gly Leu Gly Glu Asp Trp Leu Ser Ala Lys Glu 
            820                 825                 830     

<210> 7
<211> 35
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..35
<223> /organism="Artificial Sequence"
      /note="Primer HG1"
      /mol_type="unassigned DNA"

<400> 7
acatgattag caaaagggcc tagcttggac tcaga                                35


<210> 8
<211> 35
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..35
<223> /organism="Artificial Sequence"
      /note="Primer HG2"
      /mol_type="unassigned DNA"

<400> 8
tgcacctgct ctgtgattat gactatccca cagtc                                35


<210> 9
<211> 2499
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..2499
<223> /organism="Artificial Sequence"
      /note="Nucleic acid sequence of the improved DNA-Polymerase
      variant SD DNA Polymerase (SEQ ID NO:9)"
      /mol_type="unassigned DNA"

<400> 9
atgaggggga tgctgcccct ctttgagccc aagggccggg tcctcctggt ggacggccac      60

cacctggcct accgcacctt ccacgccctg aagggcctca ccaccagccg gggggagccg     120

gtgcaggcgg tctacgactt cgccaagagc ctcctcaagg ccctcaagga ggacggggac     180

gcggtgatcg tggtctttga cgccaaggcc ccctccttcc gccacgaggc ctacgggggg     240

tacaaggcgg gccgggcccc cacgccggag gactttcccc ggcaactcgc cctcatcaag     300

gagctggtgg acctcctggg gctggcgcgc ctcgaggtcc cgggctacga ggcggacgac     360

gtcctggcca gcctggccaa gaaggcggaa aaggagggct acgaggtccg catcctcacc     420

gccgacaaag acctttacca gctcctttcc gaccgcatcc acgtcctcca ccccgagggg     480

tacctcatca ccccggcctg gctttgggaa aagtacggcc tgaggcccga ccagtgggcc     540

gactaccggg ccctgaccgg ggacgagtcc gacaaccttc ccggggtcaa gggcatcggg     600

gagaagacgg cgaggaagct tctggaggag tgggggagcc tggaagccct cctcaagaac     660

ctggaccggc tgaagcccgc catccgggag aagatcctgg cccacatgga cgatctgaag     720

ctctcctggg acctggccaa ggtgcgcacc gacctgcccc tggaggtgga cttcgccaaa     780

aggcgggagc ccgaccggga gaggcttagg gcctttctgg agaggcttga gtttggcagc     840

ctcctccacg agttcggcct tctggaaagc cccaaggccc tggaggaggc cccctggccc     900

ccgccggaag gggccttcgt gggctttgtg ctttcccgca aggagcccat gtgggccgat     960

cttctggccc tggccgccgc cagggggggc cgggtccacc gggcccccga gccttataaa    1020

gccctcaggg acctgaagga ggcgcggggg cttctcgcca aagacctgag cgttctggcc    1080

ctgagggaag gccttggcct cccgcccggc gacgacccca tgctcctcgc ctacctcctg    1140

gacccttcca acaccacccc cgagggggtg gcccggcgct acggcgggga gtggacggag    1200

gaggcggggg agcgggccgc cctttccgag aggctcttcg ccaacctgtg ggggaggctt    1260

gagggggagg agaggctcct ttggctttac cgggaggtgg agaggcccct ttccgctgtc    1320

ctggcccaca tggaggccac gggggtgcgc ctggacgtgg cctatctcag ggccttgtcc    1380

ctggaggtgg ccgaggagat cgcccgcctc gaggccgagg tcttccgcct ggccggccac    1440

cccttcaacc tcaactcccg ggaccagctg gaaagggtcc tctttgacga gctagggctt    1500

cccgccatcg gcaagacgga gaagaccggc aagcgctcca ccagcgccgc cgtcctggag    1560

gccctccgcg aggcccaccc catcgtggag aagatcctgc agtaccggga gctcaccaag    1620

ctgaagagca cctacattga ccccttgccg gacctcatcc accccaggac gggccgcctc    1680

cacacccgct tcaaccagac ggccacggcc acgggcaggc taagtagctc cgatcccaac    1740

ctccagaaca tccccgtccg caccccgctt gggcagagga tccgccgggc cttcatcgcc    1800

gaggaggggt ggctattggt ggccctggac tatagccaga tagagctcag ggtgctggcc    1860

cacattgcgg atgacgataa cctgatcgaa gcctttcagc gcgatctgga cattcatacg    1920

aaaaccgcga tggatatctt ccatgtgagc gaagaggaag tgaccgcgaa tatgcggcgc    1980

caagccaaag cggttaacta tggcattgtg tatggcatca gcgattatgg tctggcgcag    2040

aatctgaaca ttacccgcaa agaggcagcg gaattcattg agcgctactt tcagagcttc    2100

cccaaggtgc gggcctggat tgagaagacc ctggaggagg gcaggaggcg ggggtacgtg    2160

gagaccctct tcggccgccg ccgctacgtg ccagacctag aggcccggaa ctttaacgtg    2220

cgcagctttg cggaacgcac cgcgatgaac acccccgtcc agggcaccgc tgccgacctc    2280

atgaagctgg ctatggtgaa gctcttcccc aggctggagg aaatgggggc caggatgctc    2340

cttcaggtcc acgacgagct ggtcctcgag gccccaaaag agagggcgga ggccgtggcc    2400

cggctggcca aggaggtcat ggagggggtg tatcccctgg ccgtgcccct ggaggtggag    2460

gtggggatag gggaggactg gctctccgcc aaggagtga                           2499


<210> 10
<211> 2502
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..2502
<223> /organism="Artificial Sequence"
      /note="Nucleic acid sequence of a chimeric DNA-Polymerase variant
      (SEQ ID NO:10), derived from a parent Tth DNA-Polymerase and Bst
      Polymerase I"
      /mol_type="unassigned DNA"

<400> 10
atggaagcga tgctgccgct gtttgaaccg aaaggccgtg tgctgctggt ggatggccat      60

catctggcgt atcgtacctt ttttgcgctg aaaggcctga ccacctcccg tggcgaaccg     120

gtgcaggcgg tgtatgattt tgcgaaatcc ctgctgaaag cgctgaaaga agatggctat     180

aaagcggtgt ttgtggtgtt tgatgcgaaa gcgccgtcct ttcgtcatga agcgtatgaa     240

gcgtataaag cgggccgtgc gccgaccccg gaagattttc cgcgtcagct ggcgctgatt     300

aaagaactgg tggatctgct gggctttacc cgtctggaag tgccgggcta tgaagcggat     360

gatgtgctgg cgaccctggc gaaaaaagcg gaaaaagaag gctatgaagt gcgtattctg     420

accgcggatc gtgatctgta tcagctggtg tccgatcgtg tggcggtgct gcatccggaa     480

ggccatctga ttaccccgga atggctgtgg gaaaaatatg gcctgcgtcc ggaacagtgg     540

gtggattttc gtgcgctggt gggcgatccg tccgataatc tgccgggcgt gaaaggcatt     600

ggcgaaaaaa ccgcgctgaa actgctgaaa gaatggggct ccctggaaaa tctgctgaaa     660

aatctggatc gtgtgaaacc ggaaaatgtg cgtgaaaaaa ttaaagcgca tctggaagat     720

ctgcgtctgt ccctggaact gtcccgtgtg cgtaccgatc tgccgctgga agtggatctg     780

gcgcagggcc gtgaaccgga tcgtgaaggc ctgcgtgcgt ttctggaacg tctggaattt     840

ggctccctgc tgcatgaatt tggcctgctg gaagcgccgg cgccgctgga agaagcgccg     900

tggccgccgc cggaaggcgc gtttgtgggc tttgtgctgt cccgtccgga accgatgtgg     960

gcggaactga aagcgctggc ggcgtgccgt gatggccgtg tgcatcgtgc ggcggatccg    1020

ctggcgggcc tgaaagatct gaaagaagtg cgtggcctgc tggcgaaaga tctggcggtg    1080

ctggcgtccc gtgaaggcct ggatctggtg ccgggcgatg atccgatgct gctggcgtat    1140

ctgctggatc cgtccaatac caccccggaa ggcgtggcgc gtcgttatgg cggcgaatgg    1200

accgaagatg cggcgcatcg tgcgctgctg tccgaacgtc tgcatcgtaa tctgctgaaa    1260

cgtctggaag gcgaagaaaa actgctgtgg ctgtatcatg aagtggaaaa accgctgtcc    1320

cgtgtgctgg cgcatatgga agcgaccggc gtgcgtcgtg atgtggcgta tctgcaggcg    1380

ctgtccctgg aactggcgga agaaattcgt cgtctggaag aagaagtgtt tcgtctggcg    1440

ggccatccgt ttaatctgaa ttcccgtgat cagctggaac gtgtgctgtt tgatgaactg    1500

cgtctgccgg cgctgggcaa aacccagaaa accggcaaac gttccacctc cgcggcggtg    1560

ctggaagcgc tgcgtgaagc gcatccgatt gtggaaaaaa ttctgcagca tcgtgaactg    1620

accaaactga aaaataccta tgtggatccg ctgccgtccc tggtgcatcc gcgtaccggc    1680

cgtctgcata cccgttttaa tcagaccgcg accgcgaccg gccgtctgtc ctcctccgat    1740

ccgaatctgc agaatattcc ggtgcgtacc ccgctgggcc agcgtattcg tcgtgcgttt    1800

gtggcggaag cgggctgggc gctggtggcg ctggattatt cccagattga actgcgtgtg    1860

ctggcgcata ttgcggatga tgataatctg attgaagcgt ttcagcgtga tctggatatt    1920

cataccaaaa ccgcgatgga tatttttcat gtgtccgaag aagaagtgac cgcgaatatg    1980

cgtcgtcagg cgaaagcggt gaattttggc attgtgtatg gcatttccga ttatggcctg    2040

gcgcagaatc tgaatattac ccgtaaagaa gcggcggaat ttattgaacg ttattttcag    2100

tcctttccga aagtgcgtgc gtggattgaa aaaaccctgg aagaaggccg taaacgtggc    2160

tatgtggaaa ccctgtttgg ccgtcgtcgt tatgtgccgg atctgaatgc gcgtaatttt    2220

aatgtgcgtt cctttgcgga acgtaccgcg atgaataccc cggtgcaggg caccgcggcg    2280

gatctgatga aactggcgat ggtgaaactg tttccgcgtc tgcgtgaaat gggcgcgcgt    2340

atgctgctgc aggtgcatga tgaactgctg ctggaagcgc cgcaggcgcg tgcggaagaa    2400

gtggcggcgc tggcgaaaga agcgatggaa aaagcgtatc cgctggcggt gccgctggaa    2460

gtggaagtgg gcatgggcga agattggctg tccgcgaaag gc                       2502


<210> 11
<211> 2493
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..2493
<223> /organism="Artificial Sequence"
      /note="Nucleic acid sequence of a chimeric DNA-Polymerase variant
      (SEQ ID NO:11), derived from a parent Tfl DNA-Polymerase and Bst
      Polymerase I"
      /mol_type="unassigned DNA"

<400> 11
atggcgatgc tgccgctgtt tgaaccgaaa ggccgtgtgc tgctggtgga tggccatcat      60

ctggcgtatc gtaccttttt tgcgctgaaa ggcctgacca cctcccgtgg cgaaccggtg     120

caggcggtgt atgattttgc gaaatccctg ctgaaagcgc tgaaagaaga tggcgatgtg     180

gtggtggtgg tgtttgatgc gaaagcgccg tcctttcgtc atgaagcgta tgaagcgtat     240

aaagcgggcc gtgcgccgac cccggaagat tttccgcgtc agctggcgct gattaaagaa     300

ctggtggatc tgctgggcct ggtgcgtctg gaagtgccgg gctttgaagc ggatgatgtg     360

ctggcgaccc tggcgaaacg tgcggaaaaa gaaggctatg aagtgcgtat tctgaccgcg     420

gatcgtgatc tgtatcagct gctgtccgaa cgtattgcga ttctgcatcc ggaaggctat     480

ctgattaccc cggcgtggct gtatgaaaaa tatggcctgc gtccggaaca gtgggtggat     540

tatcgtgcgc tggcgggcga tccgtccgat aatattccgg gcgtgaaagg cattggcgaa     600

aaaaccgcgc agcgtctgat tcgtgaatgg ggctccctgg aaaatctgtt tcagcatctg     660

gatcaggtga aaccgtccct gcgtgaaaaa ctgcaggcgg gcatggaagc gctggcgctg     720

tcccgtaaac tgtcccaggt gcataccgat ctgccgctgg aagtggattt tggccgtcgt     780

cgtaccccga atctggaagg cctgcgtgcg tttctggaac gtctggaatt tggctccctg     840

ctgcatgaat ttggcctgct ggaaggcccg aaagcggcgg aagaagcgcc gtggccgccg     900

ccggaaggcg cgtttctggg cttttccttt tcccgtccgg aaccgatgtg ggcggaactg     960

ctggcgctgg cgggcgcgtg ggaaggccgt ctgcatcgtg cgcaggatcc gctgcgtggc    1020

ctgcgtgatc tgaaaggcgt gcgtggcatt ctggcgaaag atctggcggt gctggcgctg    1080

cgtgaaggcc tggatctgtt tccggaagat gatccgatgc tgctggcgta tctgctggat    1140

ccgtccaata ccaccccgga aggcgtggcg cgtcgttatg gcggcgaatg gaccgaagat    1200

gcgggcgaac gtgcgctgct ggcggaacgt ctgtttcaga ccctgaaaga acgtctgaaa    1260

ggcgaagaac gtctgctgtg gctgtatgaa gaagtggaaa aaccgctgtc ccgtgtgctg    1320

gcgcgtatgg aagcgaccgg cgtgcgtctg gatgtggcgt atctgcaggc gctgtccctg    1380

gaagtggaag cggaagtgcg tcagctggaa gaagaagtgt ttcgtctggc gggccatccg    1440

tttaatctga attcccgtga tcagctggaa cgtgtgctgt ttgatgaact gggcctgccg    1500

gcgattggca aaaccgaaaa aaccggcaaa cgttccacct ccgcggcggt gctggaagcg    1560

ctgcgtgaag cgcatccgat tgtggatcgt attctgcagt atcgtgaact gaccaaactg    1620

aaaaatacct atattgatcc gctgccggcg ctggtgcatc cgaaaaccgg ccgtctgcat    1680

acccgtttta atcagaccgc gaccgcgacc ggccgtctgt cctcctccga tccgaatctg    1740

cagaatattc cggtgcgtac cccgctgggc cagcgtattc gtcgtgcgtt tgtggcggaa    1800

gaaggctggg tgctggtggt gctggattat tcccagattg aactgcgtgt gctggcgcat    1860

attgcggatg atgataatct gattgaagcg tttcagcgtg atctggatat tcataccaaa    1920

accgcgatgg atatttttca tgtgtccgaa gaagaagtga ccgcgaatat gcgtcgtcag    1980

gcgaaagcgg tgaattttgg cattgtgtat ggcatttccg attatggcct ggcgcagaat    2040

ctgaatatta cccgtaaaga agcggcggaa tttattgaac gttattttca gtcctatccg    2100

aaagtgcgtg cgtggattga aggcaccctg gaagaaggcc gtcgtcgtgg ctatgtggaa    2160

accctgtttg gccgtcgtcg ttatgtgccg gatctgaatg cgcgtaattt taatgtgcgt    2220

tcctttgcgg aacgtaccgc gatgaatacc ccggtgcagg gcaccgcggc ggatctgatg    2280

aaactggcga tggtgcgtct gtttccgcgt ctgcaggaac tgggcgcgcg tatgctgctg    2340

caggtgcatg atgaactggt gctggaagcg ccgaaagatc gtgcggaacg tgtggcggcg    2400

ctggcgaaag aagtgatgga aggcgtgtgg ccgctgcagg tgccgctgga agtggaagtg    2460

ggcctgggcg aagattggct gtccgcgaaa gaa                                 2493


<210> 12
<211> 16
<212> PRT
<213> Artificial Sequence

<220> 
<223> Amino acid sequence motif comprised in embodiments of the
      DNA-Polymerase variants

<400> 12
Ala Asn Phe Asn Val Arg Ser Phe Ala Glu Arg Thr Ala Met Asn Thr 
1               5                   10                  15      



<210> 13
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> Amino acid sequence motif comprised in embodiments of the
      DNA-Polymerase variants

<400> 13
Asx Asp Tyr Gly Xaa Xaa Xaa Xaa Xaa Xaa Ile Thr Arg Lys 
1               5                   10                  

<210> 14
<211> 13
<212> PRT
<213> Artificial Sequence

<220> 
<223> Amino acid sequence motif comprised in embodiments of the
      DNA-Polymerase variants

<400> 14
Asp Tyr Gly Leu Ala Gln Asn Leu Asn Ile Thr Arg Lys 
1               5                   10              

<210> 15
<211> 876
<212> PRT
<213> Artificial Sequence

<220> 
<223> Amino acid sequence of wild-type Bacillus stearothermophilus DNA
      Polymerase I (SEQ ID NO: 15)

<400> 15
Met Lys Lys Lys Leu Val Leu Ile Asp Gly Asn Ser Val Ala Tyr Arg 
1               5                   10                  15      
Ala Phe Phe Ala Leu Pro Leu Leu His Asn Asp Lys Gly Ile His Thr 
            20                  25                  30          
Asn Ala Val Tyr Gly Phe Thr Met Met Leu Asn Lys Ile Leu Ala Glu 
        35                  40                  45              
Glu Gln Pro Thr His Leu Leu Val Ala Phe Asp Ala Gly Lys Thr Thr 
    50                  55                  60                  
Phe Arg His Glu Thr Phe Gln Glu Tyr Lys Gly Gly Arg Gln Gln Thr 
65                  70                  75                  80  
Pro Pro Glu Leu Ser Glu Gln Phe Pro Leu Leu Arg Glu Leu Leu Lys 
                85                  90                  95      
Ala Tyr Arg Ile Pro Ala Tyr Glu Leu Asp His Tyr Glu Ala Asp Asp 
            100                 105                 110         
Ile Ile Gly Thr Leu Ala Ala Arg Ala Glu Gln Glu Gly Phe Glu Val 
        115                 120                 125             
Lys Ile Ile Ser Gly Asp Arg Asp Leu Thr Gln Leu Ala Ser Arg His 
    130                 135                 140                 
Val Thr Val Asp Ile Thr Lys Lys Gly Ile Thr Asp Ile Glu Pro Tyr 
145                 150                 155                 160 
Thr Pro Glu Thr Val Arg Glu Lys Tyr Gly Leu Thr Pro Glu Gln Ile 
                165                 170                 175     
Val Asp Leu Lys Gly Leu Met Gly Asp Lys Ser Asp Asn Ile Pro Gly 
            180                 185                 190         
Val Pro Gly Ile Gly Glu Lys Thr Ala Val Lys Leu Leu Lys Gln Phe 
        195                 200                 205             
Gly Thr Val Glu Asn Val Leu Ala Ser Ile Asp Glu Val Lys Gly Glu 
    210                 215                 220                 
Lys Leu Lys Glu Asn Leu Arg Gln His Arg Asp Leu Ala Leu Leu Ser 
225                 230                 235                 240 
Lys Gln Leu Ala Ser Ile Cys Arg Asp Ala Pro Val Glu Leu Ser Leu 
                245                 250                 255     
Asp Asp Ile Val Tyr Glu Gly Gln Asp Arg Glu Lys Val Ile Ala Leu 
            260                 265                 270         
Phe Lys Glu Leu Gly Phe Gln Ser Phe Leu Glu Lys Met Ala Ala Pro 
        275                 280                 285             
Ala Ala Glu Gly Glu Lys Pro Leu Glu Glu Met Glu Phe Ala Ile Val 
    290                 295                 300                 
Asp Val Ile Thr Glu Glu Met Leu Ala Asp Lys Ala Ala Leu Val Val 
305                 310                 315                 320 
Glu Val Met Glu Glu Asn Tyr His Asp Ala Pro Ile Val Gly Ile Ala 
                325                 330                 335     
Leu Val Asn Glu His Gly Arg Phe Phe Met Arg Pro Glu Thr Ala Leu 
            340                 345                 350         
Ala Asp Ser Gln Phe Leu Ala Trp Leu Ala Asp Glu Thr Lys Lys Lys 
        355                 360                 365             
Ser Met Phe Asp Ala Lys Arg Ala Val Val Ala Leu Lys Trp Lys Gly 
    370                 375                 380                 
Ile Glu Leu Arg Gly Val Ala Phe Asp Leu Leu Leu Ala Ala Tyr Leu 
385                 390                 395                 400 
Leu Asn Pro Ala Gln Asp Ala Gly Asp Ile Ala Ala Val Ala Lys Met 
                405                 410                 415     
Lys Gln Tyr Glu Ala Val Arg Ser Asp Glu Ala Val Tyr Gly Lys Gly 
            420                 425                 430         
Val Lys Arg Ser Leu Pro Asp Glu Gln Thr Leu Ala Glu His Leu Val 
        435                 440                 445             
Arg Lys Ala Ala Ala Ile Trp Ala Leu Glu Gln Pro Phe Met Asp Asp 
    450                 455                 460                 
Leu Arg Asn Asn Glu Gln Asp Gln Leu Leu Thr Lys Leu Glu Gln Pro 
465                 470                 475                 480 
Leu Ala Ala Ile Leu Ala Glu Met Glu Phe Thr Gly Val Asn Val Asp 
                485                 490                 495     
Thr Lys Arg Leu Glu Gln Met Gly Ser Glu Leu Ala Glu Gln Leu Arg 
            500                 505                 510         
Ala Ile Glu Gln Arg Ile Tyr Glu Leu Ala Gly Gln Glu Phe Asn Ile 
        515                 520                 525             
Asn Ser Pro Lys Gln Leu Gly Val Ile Leu Phe Glu Lys Leu Gln Leu 
    530                 535                 540                 
Pro Val Leu Lys Lys Thr Lys Thr Gly Tyr Ser Thr Ser Ala Asp Val 
545                 550                 555                 560 
Leu Glu Lys Leu Ala Pro His His Glu Ile Val Glu Asn Ile Leu His 
                565                 570                 575     
Tyr Arg Gln Leu Gly Lys Leu Gln Ser Thr Tyr Ile Glu Gly Leu Leu 
            580                 585                 590         
Lys Val Val Arg Pro Asp Thr Gly Lys Val His Thr Met Phe Asn Gln 
        595                 600                 605             
Ala Leu Thr Gln Thr Gly Arg Leu Ser Ser Ala Glu Pro Asn Leu Gln 
    610                 615                 620                 
Asn Ile Pro Ile Arg Leu Glu Glu Gly Arg Lys Ile Arg Gln Ala Phe 
625                 630                 635                 640 
Val Pro Ser Glu Pro Asp Trp Leu Ile Phe Ala Ala Asp Tyr Ser Gln 
                645                 650                 655     
Ile Glu Leu Arg Val Leu Ala His Ile Ala Asp Asp Asp Asn Leu Ile 
            660                 665                 670         
Glu Ala Phe Gln Arg Asp Leu Asp Ile His Thr Lys Thr Ala Met Asp 
        675                 680                 685             
Ile Phe His Val Ser Glu Glu Glu Val Thr Ala Asn Met Arg Arg Gln 
    690                 695                 700                 
Ala Lys Ala Val Asn Phe Gly Ile Val Tyr Gly Ile Ser Asp Tyr Gly 
705                 710                 715                 720 
Leu Ala Gln Asn Leu Asn Ile Thr Arg Lys Glu Ala Ala Glu Phe Ile 
                725                 730                 735     
Glu Arg Tyr Phe Ala Ser Phe Pro Gly Val Lys Gln Tyr Met Glu Asn 
            740                 745                 750         
Ile Val Gln Glu Ala Lys Gln Lys Gly Tyr Val Thr Thr Leu Leu His 
        755                 760                 765             
Arg Arg Arg Tyr Leu Pro Asp Ile Thr Ser Arg Asn Phe Asn Val Arg 
    770                 775                 780                 
Ser Phe Ala Glu Arg Thr Ala Met Asn Thr Pro Ile Gln Gly Ser Ala 
785                 790                 795                 800 
Ala Asp Ile Ile Lys Lys Ala Met Ile Asp Leu Ala Ala Arg Leu Lys 
                805                 810                 815     
Glu Glu Gln Leu Gln Ala Arg Leu Leu Leu Gln Val His Asp Glu Leu 
            820                 825                 830         
Ile Leu Glu Ala Pro Lys Glu Glu Ile Glu Arg Leu Cys Glu Leu Val 
        835                 840                 845             
Pro Glu Val Met Glu Gln Ala Val Thr Leu Arg Val Pro Leu Lys Val 
    850                 855                 860                 
Asp Tyr His Tyr Gly Pro Thr Trp Tyr Asp Ala Lys 
865                 870                 875     

<210> 16
<211> 1228
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..1228
<223> /organism="Artificial Sequence"
      /note="Nucleic acid sequence of murine G3PDH cDNA (SEQ ID NO:
      16)"
      /mol_type="unassigned DNA"

<400> 16
acagccgcat cttcttgtgc agtgccagcc tcgtcccgta gacaaaatgg tgaaggtcgg      60

tgtgaacgga tttggccgta ttgggcgcct ggtcaccagg gctgccattt gcagtggcaa     120

agtggagatt gttgccatca acgacccctt cattgacctc aactacatgg tctacatgtt     180

ccagtatgac tccactcacg gcaaattcaa cggcacagtc aaggccgaga atgggaagct     240

tgtcatcaac gggaagccca tcaccatctt ccaggagcga gaccccacta acatcaaatg     300

gggtgaggcc ggtgctgagt atgtcgtgga gtctactggt gtcttcacca ccatggagaa     360

ggccggggcc cacttgaagg gtggagccaa acgggtcatc atctccgccc cttctgccga     420

tgcccccatg tttgtgatgg gtgtgaacca cgagaaatat gacaactcac tcaagattgt     480

cagcaatgca tcctgcacca ccaactgctt agcccccctg gccaaggtca tccatgacaa     540

ctttggcatt gtggaagggc tcatgaccac agtccatgcc atcactgcca cccagaagac     600

tgtggatggc ccctctggaa agctgtggcg tgatggccgt ggggctgccc agaacatcat     660

ccctgcatcc actggtgctg ccaaggctgt gggcaaggtc atcccagagc tgaacgggaa     720

gctcactggc atggccttcc gtgttcctac ccccaatgtg tccgtcgtgg atctgacgtg     780

ccgcctggag aaacctgcca agtatgatga catcaagaag gtggtgaagc aggcatctga     840

gggcccactg aagggcatct tgggctacac tgaggaccag gttgtctcct gcgacttcaa     900

cagcaactcc cactcttcca ccttcgatgc cggggctggc attgctctca atgacaactt     960

tgtcaagctc atttcctggt atgacaatga atacggctac agcaacaggg tggtggacct    1020

catggcctac atggcctcca aggagtaaga aaccctggac cacccacccc agcaaggaca    1080

ctgagcaaga gaggccctat cccaactcgg cccccaacac tgagcatctc cctcacaatt    1140

tccatcccag acccccataa taacaggagg ggcctaggga gccctcccta ctctcttgaa    1200

taccatcaat aaagttcgct gcacccac                                       1228


<210> 17
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..21
<223> /organism="Artificial Sequence"
      /note="Primer F1"
      /mol_type="unassigned DNA"

<400> 17
gtgaaggtcg gtgtgaacgg a                                              21


<210> 18
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /organism="Artificial Sequence"
      /note="Primer F2"
      /mol_type="unassigned DNA"

<400> 18
ttctgccgat gcccccatgt                                                20


<210> 19
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /organism="Artificial Sequence"
      /note="Primer F3"
      /mol_type="unassigned DNA"

<400> 19
gcatcctgca ccaccaactg                                                20


<210> 20
<211> 20
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..20
<223> /organism="Artificial Sequence"
      /note="Primer R3"
      /mol_type="unassigned DNA"

<400> 20
gagcttcccg ttcagctctg                                                20


<210> 21
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..21
<223> /organism="Artificial Sequence"
      /note="Primer R2"
      /mol_type="unassigned DNA"

<400> 21
cagatccacg acggacacat t                                              21


<210> 22
<211> 21
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..21
<223> /organism="Artificial Sequence"
      /note="Primer R1"
      /mol_type="unassigned DNA"

<400> 22
ggtttcttac tccttggagg c                                              21


<210> 23
<211> 28
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..28
<223> /organism="Artificial Sequence"
      /note="AmpliFluor AF3"
      /mol_type="unassigned DNA"

<400> 23
caggatgcgc atcctgcacc accaactg                                       28


