                         SEQUENCE LISTING

<110>  Novozymes A/S
       Udagawa, Hiroaki
 
<120>  MODIFIED FUNGAL HOST CELLS AND METHOD OF USING SAME

<130>  12580-WO-PCT

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  3909
<212>  DNA
<213>  Aspergillus niger

<400>  1
atgactgctc gtgggaaaaa cgttaagacg cttgccacgg agagcgaaat ctttgtctat       60

gaccgaagat gggtcagcga gcccaacagc atccagattc ccgagctacc cccgccacat      120

ccactgcacg tcgataaccc gcccgatacc ctcacaaacc agaacgatct acaagcatgg      180

aggagcctct acttagcaag gaggacatgg gccgcggacc tcacggagcg ctgcgagtcg      240

gcggaaaagc agatacatga gcacaacgag cgcactgata tcatcaaccg agctgcaagc      300

gtagctctcg agaacctcaa aacccacgtc ggcaccctag agcataaatt ccaagaggcc      360

cagtccttcg ccaatgaact tttaagtcaa cagcaagcag cactcaatgg atggaaaagg      420

gcattgtcaa ctttagatag cattccagct cgcaaggact tccccttcct gggccgtccg      480

tcaacaccaa cgaaaggcag aggccaagcg atgggtacac tacaagacta cgtggataca      540

gcggaggtac aaagggctgg ctcggaagcc ccagaaatac ttgcgcgctt tgcccatcaa      600

gttagcacta tcgagaaaaa tgttagtgag attgctacag atactcaacg cctggttgat      660

gaagcctttc ctacgagtgt tgagcccgtg gatggactct tgcaggaaat ggaaacaata      720

tcaaataaga tacaatccga ctacgagcat gttctggcct tgcctaacaa cccaaagaca      780

ttggcaaacg tttctaggct cgctctcagc catacacaag accttctacc atcgttattg      840

gacgttagca acgaaataca ggctggccag gaagaagctg tgagacgata taatgcggca      900

gtgaaagcct cgtcgagtca tatgcggctc atatcttcta ttgagtatcg gcttgccgaa      960

actctacctc agatcaacag cctgcctttg caaagcgaag catttgatgt catttacacg     1020

gtcttccata tgcctattat ctacgggtcg gttctgattg agtctgtgcg acgtcgcgag     1080

ttcaatgata aaatgaagaa cgactcactg actttggcgg aggaaatgtc tgtattccgg     1140

gaagaagagc agcgtcgaag gaagaaatgg ctcaaaaata tggctgattt tatttccatc     1200

tctgatgcta cgacccctgg catcgagata aatctacggg gccaggagca ggaatggcct     1260

gccgttagta ggaaagaagt tgagtcatat atcgaagacc tgaaaaataa acctgacatg     1320

gaaaatcacg cccaagaact gatgcagcgc ttcaaagagc ttgatgctcc tacccggatg     1380

cagcgtcgca gagcaaaagc tttcaaacag ggaagtcttt ttgacctgag ccgaagctcg     1440

cttttactgc gcagtgacga tatggtcaga agtctaaaag atgagaaagt caagcttgaa     1500

gatagattga aaggctccga gagccgtatt cgaaagttag aggatctcct tcatcgtcag     1560

agtcatatga gccgaccctc cagcggcaac ttctcacttg atttccccag ttcaccggcg     1620

tcgcctcacc ccgactcact ttcaaggaga tcttcggtct cttcaaggcg aatgtctgca     1680

aatcaagctc cggaggaaaa ggcgctcgta caacgtatca ctcgcctcga ggccgagcta     1740

gcggctgagc gagacacggt gcaacgactt caaaaggagg cacatgccga gcggcagtct     1800

aataccgaca aaatacaaga agcccagtca acgaagaaag atctcattgg caatctcgaa     1860

gcgcggcaac gtgagtttga tgacgagcgc agatttttgg agggtgaatt aagaaggctt     1920

cgaatccgga ctgaggagct agaagaggag gtggacagaa atctcgacag tcgcgaacac     1980

gagaagcaag acgccgatga gcgcatacat catctcgaga tggaattgca agatgtccat     2040

gcccgagctg aagaagaatt gaggaaagca aatgaaacga ttggacactt gcaatcggag     2100

aagggcgtag gagaaaccct acgggcccgt attgaagagc tacagaacca ggtggctgaa     2160

aacgaaagga aagaaaacga taacaagcac gctttgcagg tcgcttttct caatttgtct     2220

cctggtggct ctgttcctac cgaatttccg aatatcatta aagcaattga ggttctctcc     2280

gaagggctaa caatccatgc caagaatgcg gaggagagtg ccgcaaaagc ttcagcggaa     2340

agcaaggtgc tcgaagaacg gttgggtctg atggagtctg aattggagga tttgagaaag     2400

actgcgacct cacgagaaac agaactttcc caagctcgaa atgaattggc tgaaggaaaa     2460

gcaaagctga gcattgtgac ctcagaattg gaagatgaac gatcaaaact catcgcatta     2520

cagtcccaat ttgctgcggg cgagacagga tccgacgccc tgcgggagcg tgttaccgaa     2580

gaagaacgga agttaactga tctggcccag aggctagctg aggttgagac attggctcgt     2640

cagtctgaag aagaagttac tacctggaag aagaaggttg aagcaatgtc cgaagcggag     2700

cagcaaacta ctgtacggtt cgagacttgc ggggtcaggt tccaggagtt gtcaaagcag     2760

cttttcgcac aagtggagaa gctggagcgc atgctggaac aactagggtt cacgatcgtt     2820

cgtcaaaatg gtgacatagt tgtgcagagg tcgtccaagg tcaatgccct ctctagcacc     2880

ggtgacagtc ttagccagtc gggagtggtg tctgtgagac cggacgcaag ccttctcgat     2940

tggatgcatg ccgatagcaa ggaagaagaa acagataagt tcatggcttt cgtggaatcc     3000

ttatatcaat ttgatgtgga catcttcggc gacgcggtcg tcaagcgtgt aaaggatatt     3060

gaagttcttg ctcgcaaatg gcagaaagaa gcccgcggat accgagacaa gtaccaccgg     3120

gttcaaagcg aagcccatga taaaatcgcc taccggtcct ttaaagaagg ggatcttgca     3180

ctcttcttgc ctacccgcaa ccaagctatc cggtcatggg ctgcgttcaa tgttggcgca     3240

ccacactatt tcctgcgcga gcaagatgcg cacaagcttc aaacccggga ctggctgctt     3300

gctcggatta ccaagatcga agaacgcgtt gttgatctct ccaaatcgat gaacggtgcc     3360

aatcctgacc gccgttccat cggcgaagtc agcgatggca cctcacttga tgacgaaaac     3420

ccgtttgagc tgtctgatgg tctccgctgg tacctcttag atgccacgga agaaaagcca     3480

ggcgccccag cgactccggg cttaggaaag agcactgttg cgccagcaca cgtagacgcc     3540

aaaggcagca ttcgcatgaa acgcacatca gccggtggga atattgccaa aacgttgacc     3600

aagagcttag acagccgacg aaatagttcc aattctaaga aggggcattc ctcgactcct     3660

tcacaacgcg gcaacgaatc aacaacagac ctagcaagac tagctgaaca agacactgct     3720

gccagcttac actcacggga ggcagccccc aaacccgatg agattttaga gatctttgcg     3780

gttggaagaa cagagtataa agctagttta caggttgaac ctaaattaga tgccctttgg     3840

aacgaaagat ctcctgctcc tgaatgccgg gcaaacgagg cagtcaatta cgctaagaaa     3900

ggagagtga                                                             3909


<210>  2
<211>  1302
<212>  PRT
<213>  Aspergillus niger

<400>  2

Met Thr Ala Arg Gly Lys Asn Val Lys Thr Leu Ala Thr Glu Ser Glu 
1               5                   10                  15      


Ile Phe Val Tyr Asp Arg Arg Trp Val Ser Glu Pro Asn Ser Ile Gln 
            20                  25                  30          


Ile Pro Glu Leu Pro Pro Pro His Pro Leu His Val Asp Asn Pro Pro 
        35                  40                  45              


Asp Thr Leu Thr Asn Gln Asn Asp Leu Gln Ala Trp Arg Ser Leu Tyr 
    50                  55                  60                  


Leu Ala Arg Arg Thr Trp Ala Ala Asp Leu Thr Glu Arg Cys Glu Ser 
65                  70                  75                  80  


Ala Glu Lys Gln Ile His Glu His Asn Glu Arg Thr Asp Ile Ile Asn 
                85                  90                  95      


Arg Ala Ala Ser Val Ala Leu Glu Asn Leu Lys Thr His Val Gly Thr 
            100                 105                 110         


Leu Glu His Lys Phe Gln Glu Ala Gln Ser Phe Ala Asn Glu Leu Leu 
        115                 120                 125             


Ser Gln Gln Gln Ala Ala Leu Asn Gly Trp Lys Arg Ala Leu Ser Thr 
    130                 135                 140                 


Leu Asp Ser Ile Pro Ala Arg Lys Asp Phe Pro Phe Leu Gly Arg Pro 
145                 150                 155                 160 


Ser Thr Pro Thr Lys Gly Arg Gly Gln Ala Met Gly Thr Leu Gln Asp 
                165                 170                 175     


Tyr Val Asp Thr Ala Glu Val Gln Arg Ala Gly Ser Glu Ala Pro Glu 
            180                 185                 190         


Ile Leu Ala Arg Phe Ala His Gln Val Ser Thr Ile Glu Lys Asn Val 
        195                 200                 205             


Ser Glu Ile Ala Thr Asp Thr Gln Arg Leu Val Asp Glu Ala Phe Pro 
    210                 215                 220                 


Thr Ser Val Glu Pro Val Asp Gly Leu Leu Gln Glu Met Glu Thr Ile 
225                 230                 235                 240 


Ser Asn Lys Ile Gln Ser Asp Tyr Glu His Val Leu Ala Leu Pro Asn 
                245                 250                 255     


Asn Pro Lys Thr Leu Ala Asn Val Ser Arg Leu Ala Leu Ser His Thr 
            260                 265                 270         


Gln Asp Leu Leu Pro Ser Leu Leu Asp Val Ser Asn Glu Ile Gln Ala 
        275                 280                 285             


Gly Gln Glu Glu Ala Val Arg Arg Tyr Asn Ala Ala Val Lys Ala Ser 
    290                 295                 300                 


Ser Ser His Met Arg Leu Ile Ser Ser Ile Glu Tyr Arg Leu Ala Glu 
305                 310                 315                 320 


Thr Leu Pro Gln Ile Asn Ser Leu Pro Leu Gln Ser Glu Ala Phe Asp 
                325                 330                 335     


Val Ile Tyr Thr Val Phe His Met Pro Ile Ile Tyr Gly Ser Val Leu 
            340                 345                 350         


Ile Glu Ser Val Arg Arg Arg Glu Phe Asn Asp Lys Met Lys Asn Asp 
        355                 360                 365             


Ser Leu Thr Leu Ala Glu Glu Met Ser Val Phe Arg Glu Glu Glu Gln 
    370                 375                 380                 


Arg Arg Arg Lys Lys Trp Leu Lys Asn Met Ala Asp Phe Ile Ser Ile 
385                 390                 395                 400 


Ser Asp Ala Thr Thr Pro Gly Ile Glu Ile Asn Leu Arg Gly Gln Glu 
                405                 410                 415     


Gln Glu Trp Pro Ala Val Ser Arg Lys Glu Val Glu Ser Tyr Ile Glu 
            420                 425                 430         


Asp Leu Lys Asn Lys Pro Asp Met Glu Asn His Ala Gln Glu Leu Met 
        435                 440                 445             


Gln Arg Phe Lys Glu Leu Asp Ala Pro Thr Arg Met Gln Arg Arg Arg 
    450                 455                 460                 


Ala Lys Ala Phe Lys Gln Gly Ser Leu Phe Asp Leu Ser Arg Ser Ser 
465                 470                 475                 480 


Leu Leu Leu Arg Ser Asp Asp Met Val Arg Ser Leu Lys Asp Glu Lys 
                485                 490                 495     


Val Lys Leu Glu Asp Arg Leu Lys Gly Ser Glu Ser Arg Ile Arg Lys 
            500                 505                 510         


Leu Glu Asp Leu Leu His Arg Gln Ser His Met Ser Arg Pro Ser Ser 
        515                 520                 525             


Gly Asn Phe Ser Leu Asp Phe Pro Ser Ser Pro Ala Ser Pro His Pro 
    530                 535                 540                 


Asp Ser Leu Ser Arg Arg Ser Ser Val Ser Ser Arg Arg Met Ser Ala 
545                 550                 555                 560 


Asn Gln Ala Pro Glu Glu Lys Ala Leu Val Gln Arg Ile Thr Arg Leu 
                565                 570                 575     


Glu Ala Glu Leu Ala Ala Glu Arg Asp Thr Val Gln Arg Leu Gln Lys 
            580                 585                 590         


Glu Ala His Ala Glu Arg Gln Ser Asn Thr Asp Lys Ile Gln Glu Ala 
        595                 600                 605             


Gln Ser Thr Lys Lys Asp Leu Ile Gly Asn Leu Glu Ala Arg Gln Arg 
    610                 615                 620                 


Glu Phe Asp Asp Glu Arg Arg Phe Leu Glu Gly Glu Leu Arg Arg Leu 
625                 630                 635                 640 


Arg Ile Arg Thr Glu Glu Leu Glu Glu Glu Val Asp Arg Asn Leu Asp 
                645                 650                 655     


Ser Arg Glu His Glu Lys Gln Asp Ala Asp Glu Arg Ile His His Leu 
            660                 665                 670         


Glu Met Glu Leu Gln Asp Val His Ala Arg Ala Glu Glu Glu Leu Arg 
        675                 680                 685             


Lys Ala Asn Glu Thr Ile Gly His Leu Gln Ser Glu Lys Gly Val Gly 
    690                 695                 700                 


Glu Thr Leu Arg Ala Arg Ile Glu Glu Leu Gln Asn Gln Val Ala Glu 
705                 710                 715                 720 


Asn Glu Arg Lys Glu Asn Asp Asn Lys His Ala Leu Gln Val Ala Phe 
                725                 730                 735     


Leu Asn Leu Ser Pro Gly Gly Ser Val Pro Thr Glu Phe Pro Asn Ile 
            740                 745                 750         


Ile Lys Ala Ile Glu Val Leu Ser Glu Gly Leu Thr Ile His Ala Lys 
        755                 760                 765             


Asn Ala Glu Glu Ser Ala Ala Lys Ala Ser Ala Glu Ser Lys Val Leu 
    770                 775                 780                 


Glu Glu Arg Leu Gly Leu Met Glu Ser Glu Leu Glu Asp Leu Arg Lys 
785                 790                 795                 800 


Thr Ala Thr Ser Arg Glu Thr Glu Leu Ser Gln Ala Arg Asn Glu Leu 
                805                 810                 815     


Ala Glu Gly Lys Ala Lys Leu Ser Ile Val Thr Ser Glu Leu Glu Asp 
            820                 825                 830         


Glu Arg Ser Lys Leu Ile Ala Leu Gln Ser Gln Phe Ala Ala Gly Glu 
        835                 840                 845             


Thr Gly Ser Asp Ala Leu Arg Glu Arg Val Thr Glu Glu Glu Arg Lys 
    850                 855                 860                 


Leu Thr Asp Leu Ala Gln Arg Leu Ala Glu Val Glu Thr Leu Ala Arg 
865                 870                 875                 880 


Gln Ser Glu Glu Glu Val Thr Thr Trp Lys Lys Lys Val Glu Ala Met 
                885                 890                 895     


Ser Glu Ala Glu Gln Gln Thr Thr Val Arg Phe Glu Thr Cys Gly Val 
            900                 905                 910         


Arg Phe Gln Glu Leu Ser Lys Gln Leu Phe Ala Gln Val Glu Lys Leu 
        915                 920                 925             


Glu Arg Met Leu Glu Gln Leu Gly Phe Thr Ile Val Arg Gln Asn Gly 
    930                 935                 940                 


Asp Ile Val Val Gln Arg Ser Ser Lys Val Asn Ala Leu Ser Ser Thr 
945                 950                 955                 960 


Gly Asp Ser Leu Ser Gln Ser Gly Val Val Ser Val Arg Pro Asp Ala 
                965                 970                 975     


Ser Leu Leu Asp Trp Met His Ala Asp Ser Lys Glu Glu Glu Thr Asp 
            980                 985                 990         


Lys Phe Met Ala Phe Val Glu Ser  Leu Tyr Gln Phe Asp  Val Asp Ile 
        995                 1000                 1005             


Phe Gly  Asp Ala Val Val Lys  Arg Val Lys Asp Ile  Glu Val Leu 
    1010                 1015                 1020             


Ala Arg  Lys Trp Gln Lys Glu  Ala Arg Gly Tyr Arg  Asp Lys Tyr 
    1025                 1030                 1035             


His Arg  Val Gln Ser Glu Ala  His Asp Lys Ile Ala  Tyr Arg Ser 
    1040                 1045                 1050             


Phe Lys  Glu Gly Asp Leu Ala  Leu Phe Leu Pro Thr  Arg Asn Gln 
    1055                 1060                 1065             


Ala Ile  Arg Ser Trp Ala Ala  Phe Asn Val Gly Ala  Pro His Tyr 
    1070                 1075                 1080             


Phe Leu  Arg Glu Gln Asp Ala  His Lys Leu Gln Thr  Arg Asp Trp 
    1085                 1090                 1095             


Leu Leu  Ala Arg Ile Thr Lys  Ile Glu Glu Arg Val  Val Asp Leu 
    1100                 1105                 1110             


Ser Lys  Ser Met Asn Gly Ala  Asn Pro Asp Arg Arg  Ser Ile Gly 
    1115                 1120                 1125             


Glu Val  Ser Asp Gly Thr Ser  Leu Asp Asp Glu Asn  Pro Phe Glu 
    1130                 1135                 1140             


Leu Ser  Asp Gly Leu Arg Trp  Tyr Leu Leu Asp Ala  Thr Glu Glu 
    1145                 1150                 1155             


Lys Pro  Gly Ala Pro Ala Thr  Pro Gly Leu Gly Lys  Ser Thr Val 
    1160                 1165                 1170             


Ala Pro  Ala His Val Asp Ala  Lys Gly Ser Ile Arg  Met Lys Arg 
    1175                 1180                 1185             


Thr Ser  Ala Gly Gly Asn Ile  Ala Lys Thr Leu Thr  Lys Ser Leu 
    1190                 1195                 1200             


Asp Ser  Arg Arg Asn Ser Ser  Asn Ser Lys Lys Gly  His Ser Ser 
    1205                 1210                 1215             


Thr Pro  Ser Gln Arg Gly Asn  Glu Ser Thr Thr Asp  Leu Ala Arg 
    1220                 1225                 1230             


Leu Ala  Glu Gln Asp Thr Ala  Ala Ser Leu His Ser  Arg Glu Ala 
    1235                 1240                 1245             


Ala Pro  Lys Pro Asp Glu Ile  Leu Glu Ile Phe Ala  Val Gly Arg 
    1250                 1255                 1260             


Thr Glu  Tyr Lys Ala Ser Leu  Gln Val Glu Pro Lys  Leu Asp Ala 
    1265                 1270                 1275             


Leu Trp  Asn Glu Arg Ser Pro  Ala Pro Glu Cys Arg  Ala Asn Glu 
    1280                 1285                 1290             


Ala Val  Asn Tyr Ala Lys Lys  Gly Glu 
    1295                 1300         


<210>  3
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer 3atg11F

<400>  3
actagttcta gagttgagac attggctcgt                                        30


<210>  4
<211>  29
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer 3atg11R

<400>  4
gaattcgcta gccgtccaga gacctaggg                                         29


<210>  5
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer 5atg11F

<400>  5
gcggccgcgg tcggcgttcg aagg                                              24


<210>  6
<211>  30
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer 5atg11R

<400>  6
tactagtggg ggtatcgaag tcttcggggc                                        30


<210>  7
<211>  30
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer PE001-F

<400>  7
cggatccacc atgcgtctca ctctattatc                                        30


<210>  8
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer PE001-R

<400>  8
accacgtgtc aaaactgcca cacgtcgt                                          28


<210>  9
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer hacA1

<400>  9
tttgctagcc gacggggatg ctttttgc                                          28


<210>  10
<211>  33
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer hacA2

<400>  10
cttccatcat ggtggatcct tcaagcgtga cag                                    33


<210>  11
<211>  33
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer hacA3

<400>  11
ctgtcacgct tgaaggatcc accatgatgg aag                                    33


<210>  12
<211>  30
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer hacA4

<400>  12
ccagcgacgg acactgcagg atgttgtgtc                                        30


<210>  13
<211>  30
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer hacA5

<400>  13
gacacaacat cctgcagtgt ccgtcgctgg                                        30


<210>  14
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer hacA6

<400>  14
ttcacgtgat aaaattataa ggatt                                             25


<210>  15
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  AMG forward primer

<400>  15
ttatacatgg actcgtgact                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  AMG reverse primer

<400>  16
agaatcacag gcatcgcagg                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  hacA forward primer

<400>  17
agaagagaaa gtcatggggc                                                   20


<210>  18
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  hacA reverse primer

<400>  18
agggcacttc cttctccttc                                                   20


