                         SEQUENCE LISTING

<110>  Viventia Bio Inc
 
<120>  Compositions and Methods for Detection and Treatment of 
       Hepatocellular Carcinoma

<130>  11169-00001

<140>  PCT/CA2014/
<141>  2014-04-14

<150>  US 61/811,360
<151>  2013-04-12

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  229
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variable light chain and heavy chain sequence of antibody 
       fragment binding to EpCAM

<400>  1

His Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val 
1               5                   10                  15      


Gly Asp Arg Val Thr Ile Thr Cys Arg Ser Thr Lys Ser Leu Leu His 
            20                  25                  30          


Ser Asn Gly Ile Thr Tyr Leu Tyr Trp Tyr Gln Gln Lys Pro Gly Lys 
        35                  40                  45              


Ala Pro Lys Leu Leu Ile Tyr Gln Met Ser Asn Leu Ala Ser Gly Val 
    50                  55                  60                  


Pro Ser Arg Phe Ser Ser Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Ala Gln 
                85                  90                  95      


Asn Leu Glu Ile Pro Arg Thr Phe Gly Gln Gly Thr Lys Val Glu Leu 
            100                 105                 110         


Lys Arg Glu Val Gln Leu Val Gln Ser Gly Pro Gly Leu Val Gln Pro 
        115                 120                 125             


Gly Gly Ser Val Arg Ile Ser Cys Ala Ala Ser Gly Tyr Thr Phe Thr 
    130                 135                 140                 


Asn Tyr Gly Met Asn Trp Val Lys Gln Ala Pro Gly Lys Gly Leu Glu 
145                 150                 155                 160 


Trp Met Gly Trp Ile Asn Thr Tyr Thr Gly Glu Ser Thr Tyr Ala Asp 
                165                 170                 175     


Ser Phe Lys Gly Arg Phe Thr Phe Ser Leu Asp Thr Ser Ala Ser Ala 
            180                 185                 190         


Ala Tyr Leu Gln Ile Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr 
        195                 200                 205             


Tyr Cys Ala Arg Ala Ile Lys Gly Asp Tyr Trp Gly Gln Gly Thr Leu 
    210                 215                 220                 


Leu Thr Val Ser Ser 
225                 


<210>  2
<211>  669
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sequence of immunotoxin VB4-845

<400>  2

Met Lys Tyr Leu Leu Pro Thr Ala Ala Ala Gly Leu Leu Leu Leu Ala 
1               5                   10                  15      


Ala Gln Pro Ala Met Ala His His His His His His Asp Ile Gln Met 
            20                  25                  30          


Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr 
        35                  40                  45              


Ile Thr Cys Arg Ser Thr Lys Ser Leu Leu His Ser Asn Gly Ile Thr 
    50                  55                  60                  


Tyr Leu Tyr Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu 
65                  70                  75                  80  


Ile Tyr Gln Met Ser Asn Leu Ala Ser Gly Val Pro Ser Arg Phe Ser 
                85                  90                  95      


Ser Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln 
            100                 105                 110         


Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Ala Gln Asn Leu Glu Ile Pro 
        115                 120                 125             


Arg Thr Phe Gly Gln Gly Thr Lys Val Glu Leu Lys Arg Ala Thr Pro 
    130                 135                 140                 


Ser His Asn Ser His Gln Val Pro Ser Ala Gly Gly Pro Thr Ala Asn 
145                 150                 155                 160 


Ser Gly Thr Ser Gly Ser Glu Val Gln Leu Val Gln Ser Gly Pro Gly 
                165                 170                 175     


Leu Val Gln Pro Gly Gly Ser Val Arg Ile Ser Cys Ala Ala Ser Gly 
            180                 185                 190         


Tyr Thr Phe Thr Asn Tyr Gly Met Asn Trp Val Lys Gln Ala Pro Gly 
        195                 200                 205             


Lys Gly Leu Glu Trp Met Gly Trp Ile Asn Thr Tyr Thr Gly Glu Ser 
    210                 215                 220                 


Thr Tyr Ala Asp Ser Phe Lys Gly Arg Phe Thr Phe Ser Leu Asp Thr 
225                 230                 235                 240 


Ser Ala Ser Ala Ala Tyr Leu Gln Ile Asn Ser Leu Arg Ala Glu Asp 
                245                 250                 255     


Thr Ala Val Tyr Tyr Cys Ala Arg Phe Ala Ile Lys Gly Asp Tyr Trp 
            260                 265                 270         


Gly Gln Gly Thr Leu Leu Thr Val Ser Ser Glu Phe Gly Gly Ala Pro 
        275                 280                 285             


Glu Phe Pro Lys Pro Ser Thr Pro Pro Gly Ser Ser Gly Leu Glu Gly 
    290                 295                 300                 


Gly Ser Leu Ala Ala Leu Thr Ala His Gln Ala Cys His Leu Pro Leu 
305                 310                 315                 320 


Glu Thr Phe Thr Arg His Arg Gln Pro Arg Gly Trp Glu Gln Leu Glu 
                325                 330                 335     


Gln Cys Gly Tyr Pro Val Gln Arg Leu Val Ala Leu Tyr Leu Ala Ala 
            340                 345                 350         


Arg Leu Ser Trp Asn Gln Val Asp Gln Val Ile Arg Asn Ala Leu Ala 
        355                 360                 365             


Ser Pro Gly Ser Gly Gly Asp Leu Gly Glu Ala Ile Arg Glu Gln Pro 
    370                 375                 380                 


Glu Gln Ala Arg Leu Ala Leu Thr Leu Ala Ala Ala Glu Ser Glu Arg 
385                 390                 395                 400 


Phe Val Arg Gln Gly Thr Gly Asn Asp Glu Ala Gly Ala Ala Ser Ala 
                405                 410                 415     


Asp Val Val Ser Leu Thr Cys Pro Val Ala Ala Gly Glu Cys Ala Gly 
            420                 425                 430         


Pro Ala Asp Ser Gly Asp Ala Leu Leu Glu Arg Asn Tyr Pro Thr Gly 
        435                 440                 445             


Ala Glu Phe Leu Gly Asp Gly Gly Asp Val Ser Phe Ser Thr Arg Gly 
    450                 455                 460                 


Thr Gln Asn Trp Thr Val Glu Arg Leu Leu Gln Ala His Arg Gln Leu 
465                 470                 475                 480 


Glu Glu Arg Gly Tyr Val Phe Val Gly Tyr His Gly Thr Phe Leu Glu 
                485                 490                 495     


Ala Ala Gln Ser Ile Val Phe Gly Gly Val Arg Ala Arg Ser Gln Asp 
            500                 505                 510         


Leu Asp Ala Ile Trp Arg Gly Phe Tyr Ile Ala Gly Asp Pro Ala Leu 
        515                 520                 525             


Ala Tyr Gly Tyr Ala Gln Asp Gln Glu Pro Asp Ala Arg Gly Arg Ile 
    530                 535                 540                 


Arg Asn Gly Ala Leu Leu Arg Val Tyr Val Pro Arg Ser Ser Leu Pro 
545                 550                 555                 560 


Gly Phe Tyr Arg Thr Gly Leu Thr Leu Ala Ala Pro Glu Ala Ala Gly 
                565                 570                 575     


Glu Val Glu Arg Leu Ile Gly His Pro Leu Pro Leu Arg Leu Asp Ala 
            580                 585                 590         


Ile Thr Gly Pro Glu Glu Glu Gly Gly Arg Leu Glu Thr Ile Leu Gly 
        595                 600                 605             


Trp Pro Leu Ala Glu Arg Thr Val Val Ile Pro Ser Ala Ile Pro Thr 
    610                 615                 620                 


Asp Pro Arg Asn Val Gly Gly Asp Leu Asp Pro Ser Ser Ile Pro Asp 
625                 630                 635                 640 


Lys Glu Gln Ala Ile Ser Ala Leu Pro Asp Tyr Ala Ser Gln Pro Gly 
                645                 650                 655     


Lys Pro Pro His His His His His His Lys Asp Glu Leu 
            660                 665                 


<210>  3
<211>  2084
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence of VB4-845

<400>  3
gaattcctgc aggtctatgg aacgataaat gcccatgaaa attctatttc aaggagacag       60

tcataatgaa atacctattg cctacggcag ccgctggatt gttattactc gctgcccaac      120

cagcgatggc gcaccatcat caccatcacg atatccagat gacccagtcc ccgtcctccc      180

tgagtgcttc tgttggtgac cgtgttacca tcacctgccg ttccaccaaa tccctcctgc      240

actccaacgg tatcacctac ctttattggt atcaacagaa accgggtaaa gctccgaaac      300

ttctgatcta ccagatgtcc aacctggctt ccggtgttcc gtctcgtttc tccagttctg      360

gttctggtac cgacttcacc ctgaccatct cttctctgca gccggaagac ttcgctacct      420

actactgcgc tcagaacctg gaaatcccgc gtaccttcgg tcagggtacc aaagttgaac      480

ttaagcgcgc taccccgtct cacaactccc accaggttcc atccgcaggc ggtccgactg      540

ctaactctgg aactagtgga tccgaagtac agctggttca gtccggcccg ggtcttgttc      600

aaccgggtgg ttccgttcgt atctcttgcg ctgcttctgg ttacacgttc accaactacg      660

gcatgaactg ggtcaaacag gctccgggta aaggcctgga atggatgggc tggatcaaca      720

cctacaccgg tgaatccacc tacgctgact ccttcaaagg tcgcttcact ttctccctcg      780

acacaagtgc tagtgctgca tacctccaaa tcaactcgct gcgtgcagag gatacagcag      840

tctattactg cgcccgtttc gctatcaaag gtgactactg gggtcaaggc acgctgctga      900

ccgtttcctc ggaatttggt ggcgcgccgg agttcccgaa accgtccacc ccgccgggtt      960

cttctggttt agagggcggc agcctggccg cgctgaccgc gcaccaggcc tgccacctgc     1020

cgctggagac tttcacccgt catcgccagc cgcgcggctg ggaacaactg gagcagtgcg     1080

gctatccggt gcagcggctg gtcgccctct acctggcggc gcgactgtca tggaaccagg     1140

tcgaccaggt gatccgcaac gccctggcca gccccggcag cggcggcgac ctgggcgaag     1200

cgatccgcga gcagccggag caggcccgtc tggccctgac cctggccgcc gccgagagcg     1260

agcgcttcgt ccggcagggc accggcaacg acgaggccgg cgcggccagc gccgacgtgg     1320

tgagcctgac ctgcccggtc gccgccggtg aatgcgcggg cccggcggac agcggcgacg     1380

ccctgctgga gcgcaactat cccactggcg cggagttcct cggcgacggt ggcgacgtca     1440

gcttcagcac ccgcggcacg cagaactgga cggtggagcg gctgctccag gcgcaccgcc     1500

aactggagga gcgcggctat gtgttcgtcg gctaccacgg caccttcctc gaagcggcgc     1560

aaagcatcgt cttcggcggg gtgcgcgcgc gcagccagga tctcgacgcg atctggcgcg     1620

gtttctatat cgccggcgat ccggcgctgg cctacggcta cgcccaggac caggaacccg     1680

acgcgcgcgg ccggatccgc aacggtgccc tgctgcgggt ctatgtgccg cgctccagcc     1740

tgccgggctt ctaccgcacc ggcctgaccc tggccgcgcc ggaggcggcg ggcgaggtcg     1800

aacggctgat cggccatccg ctgccgctgc gcctggacgc catcaccggc cccgaggagg     1860

aaggcgggcg cctggagacc attctcggct ggccgctggc cgagcgcacc gtggtgattc     1920

cctcggcgat ccccaccgac ccgcgcaacg tcggtggcga cctcgacccg tccagcatcc     1980

ccgacaagga acaggcgatc agcgccctgc cggactacgc cagccagccc ggcaaaccgc     2040

cgcatcacca ccatcaccat aaagacgaac tgtagtgact cgag                      2084


<210>  4
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Light chain CDR1

<400>  4

Arg Ser Thr Lys Ser Leu Leu His Ser Asn Gly Ile Thr Tyr Leu Tyr 
1               5                   10                  15      


<210>  5
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Light chain CDR2

<400>  5

Gln Met Ser Asn Leu Ala Ser 
1               5           


<210>  6
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Light chain CDR3

<400>  6

Ala Gln Asn Leu Glu Ile Pro Arg Thr 
1               5                   


<210>  7
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Heavy chain CDR1

<400>  7

Asn Tyr Gly Met Asn 
1               5   


<210>  8
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Heavy chain CDR2

<400>  8

Trp Ile Asn Thr Tyr Thr Gly Glu Ser Thr Tyr Ala Asp Ser Phe Lys 
1               5                   10                  15      


<210>  9
<211>  0
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Heavy chain CDR3

<400>  9
000
<210>  10
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Tox1 DNA primer

<400>  10
ctcggaattc ggtggcgcgc cggagttccc gaaaccgtcc accccgccgg gttcttctgg       60

ttta                                                                    64


<210>  11
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Tox2 DNA primer

<400>  11
gtcaagcttc tacagttcgt ctttatggtg atggtggtga tgcggcggtt tcccgggctg       60


<210>  12
<211>  73
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA primer

<400>  12
ctagataacg agggcaaaaa atgaaaaaga cagctatcgc gattgcagtg gcactggctg       60

gtttcgctac cgt                                                          73


<210>  13
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA primer

<400>  13
gccactgcaa tcgcgatagc tgtctttttc attttttgcc ctcgttat                    48


<210>  14
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA primer

<400>  14
agcgcaggcc gaccaccatc atcaccatca cgat                                   34


<210>  15
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA primer

<400>  15
atcgtgatgg tgatgatggt ggtcggcctg cgctacggta gcgaaaccag ccagt            55


<210>  16
<211>  2084
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized sequence of VB4-845

<400>  16
gaattcctgc aggtctatgg aacgataaat gcccatgaaa attctatttc aaggagacag       60

tcataatgaa atatctgctg ccgactgctg ctgcgggtct gctgctgctc gcggctcagc      120

ctgctatggc acaccaccat caccaccatg acatccagat gacccagtct cctagctctc      180

ttagcgcaag cgtaggtgac cgtgtgacca tcacctgccg tagcactaaa tctctgctgc      240

atagcaacgg catcacctac ctgtattggt accagcagaa accgggtaaa gctccgaaac      300

tgctgatcta ccagatgtct aacctggcta gcggtgttcc tagccgtttt agctctagcg      360

gtagcggtac tgacttcacc ctgaccatta gctctctgca gcctgaagac ttcgcgacct      420

actactgcgc ccagaacctt gaaatcccgc gtactttcgg ccagggtacc aaagtcgaac      480

tgaaacgtgc gaccccgagc cataactctc accaggttcc tagcgcaggt ggtccgactg      540

ctaactctgg cactagcggt tctgaagttc agctcgttca gtctggtccg ggtctggttc      600

agccgggtgg tagcgttcgt atctcttgcg cggcttctgg ttacaccttc actaactacg      660

gtatgaactg ggttaaacag gctccgggta aaggtctgga gtggatgggt tggattaaca      720

cctacacggg tgaatctacc tacgccgata gcttcaaagg tcgtttcacc tttagccttg      780

acacctctgc gtctgcggcg taccttcaga tcaactctct gcgtgctgag gacaccgctg      840

tttactactg cgctcgtttc gcgattaaag gtgactattg gggccagggc accctgctga      900

ccgttagctc tgagttcggt ggtgcgcctg agttccctaa accgtctacc ccgccaggtt      960

cttctggtct tgaaggtggt agcttggcag cgttgaccgc acaccaagca tgccacctgc     1020

cgctggagac cttcacccgt caccgtcagc cgcgtggttg ggaacagctg gagcagtgcg     1080

gttatccggt tcagcgtctg gtagctctgt acctggctgc tcgtctgagc tggaaccagg     1140

ttgaccaggt gatccgtaac gcgctcgcta gcccgggttc tggtggtgac ctgggtgaag     1200

ctatccgtga acagccggaa caagctcgtc tcgcgttgac ccttgctgca gcggaatctg     1260

aacgtttcgt tcgtcagggt accggtaacg atgaagctgg tgcagcgtct gcggatgtag     1320

ttagcctgac ttgcccggtt gcggctggtg aatgcgctgg tccggcagac tctggtgacg     1380

cgttgcttga acgtaactac ccgaccggtg ctgagttcct gggtgacggt ggtgacgtta     1440

gctttagcac ccgtggtacc cagaactgga ccgttgaacg tctgctgcag gctcaccgtc     1500

agcttgaaga acgtggttat gttttcgtag gttaccacgg taccttcctg gaggcggcgc     1560

agtctatcgt gttcggtggt gttcgtgctc gttcccagga ccttgacgca atttggcgcg     1620

gtttctacat cgcgggtgac ccagcgctcg cgtacggtta cgctcaggac caggaaccgg     1680

atgcgcgtgg tcgtattcgt aacggtgcac tgctgcgtgt gtacgttcct cgttctagcc     1740

tgccgggttt ctatcgtacc ggtctgaccc tggctgcccc ggaagccgcg ggtgaagttg     1800

aacgtctgat cggtcaccct ctgccgctgc gtctggatgc gatcaccggt ccggaagaag     1860

aaggtggtcg tcttgaaacc atcctgggtt ggccgttggc tgagcgtact gtagtcatcc     1920

cgtctgcgat cccgaccgac ccgcgtaacg taggtggtga ccttgacccg tctagcatcc     1980

cggataaaga acaggccatc agcgcactgc cggactacgc gtctcaaccg ggtaaaccgc     2040

cgcaccacca tcatcaccac aaagatgaac tgtagtgact cgag                      2084


