                         SEQUENCE LISTING

<110>  Base4 Innovation Limited
       Medical Research Council
 
<120>  Single nucleotide detection method

<130>  P57530WO

<150>  GB 1306445.6
<151>  2013-04-09

<160>  3     

<170>  PatentIn version 3.5

<210>  1
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  j-shaped oligonucleotide precursor


<220>
<221>  misc_feature
<222>  (74)..(75)
<223>  Phosphorothioate linkage

<400>  1
gtaggtcctg gcacagaaaa aaggaggcag tgatgttcca tgactgattt ttttttcagt       60

catggaacat cactg                                                        75


<210>  2
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Residual single-stranded oligonucleotide region of j-shaped 
       oligonucleotide

<400>  2
gtaggtcctg gcacagaaaa aaggag                                            26


<210>  3
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  single-stranded oligonucleotide


<220>
<221>  misc_feature
<222>  (5)..(6)
<223>  Thymine bases labelled with Alexa Fluor 488 via azide linker

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Thymine base labelled with BHQ-1 quencher

<220>
<221>  misc_feature
<222>  (8)..(9)
<223>  Thymine bases labelled with Alexa Fluor 488 via azide linker

<400>  3
ctcctttttt ctgtgccaga                                                   20


