               SEQUENCE LISTING

<110> Global Bioenergies
      Scientist of Fortune S.A.

<120> Alkenol dehydratase variants

<130> W1409 PCT S3

<150> EP 13 16 8380.7
<151> 2013-05-17

<150> EP 13 19 6247.4
<151> 2013-12-09

<160> 9

<170> BiSSAP 1.2

<210> 1
<211> 397
<212> PRT
<213> Castellaniella defragrans


<400> 1
Met Arg Phe Thr Leu Lys Thr Thr Ala Ile Val Ser Ala Ala Ala Leu 
1               5                   10                  15      
Leu Ala Gly Phe Gly Pro Pro Pro Arg Ala Ala Glu Leu Pro Pro Gly 
            20                  25                  30          
Arg Leu Ala Thr Thr Glu Asp Tyr Phe Ala Gln Gln Ala Lys Gln Ala 
        35                  40                  45              
Val Thr Pro Asp Val Met Ala Gln Leu Ala Tyr Met Asn Tyr Ile Asp 
    50                  55                  60                  
Phe Ile Ser Pro Phe Tyr Ser Arg Gly Cys Ser Phe Glu Ala Trp Glu 
65                  70                  75                  80  
Leu Lys His Thr Pro Gln Arg Val Ile Lys Tyr Ser Ile Ala Phe Tyr 
                85                  90                  95      
Ala Tyr Gly Leu Ala Ser Val Ala Leu Ile Asp Pro Lys Leu Arg Ala 
            100                 105                 110         
Leu Ala Gly His Asp Leu Asp Ile Ala Val Ser Lys Met Lys Cys Lys 
        115                 120                 125             
Arg Val Trp Gly Asp Trp Glu Glu Asp Gly Phe Gly Thr Asp Pro Ile 
    130                 135                 140                 
Glu Lys Glu Asn Ile Met Tyr Lys Gly His Leu Asn Leu Met Tyr Gly 
145                 150                 155                 160 
Leu Tyr Gln Leu Val Thr Gly Ser Arg Arg Tyr Glu Ala Glu His Ala 
                165                 170                 175     
His Leu Thr Arg Ile Ile His Asp Glu Ile Ala Ala Asn Pro Phe Ala 
            180                 185                 190         
Gly Ile Val Cys Glu Pro Asp Asn Tyr Phe Val Gln Cys Asn Ser Val 
        195                 200                 205             
Ala Tyr Leu Ser Leu Trp Val Tyr Asp Arg Leu His Gly Thr Asp Tyr 
    210                 215                 220                 
Arg Ala Ala Thr Arg Ala Trp Leu Asp Phe Ile Gln Lys Asp Leu Ile 
225                 230                 235                 240 
Asp Pro Glu Arg Gly Ala Phe Tyr Leu Ser Tyr His Pro Glu Ser Gly 
                245                 250                 255     
Ala Val Lys Pro Trp Ile Ser Ala Tyr Thr Thr Ala Trp Thr Leu Ala 
            260                 265                 270         
Met Val His Gly Met Asp Pro Ala Phe Ser Glu Arg Tyr Tyr Pro Arg 
        275                 280                 285             
Phe Lys Gln Thr Phe Val Glu Val Tyr Asp Glu Gly Arg Lys Ala Arg 
    290                 295                 300                 
Val Arg Glu Thr Ala Gly Thr Asp Asp Ala Asp Gly Gly Val Gly Leu 
305                 310                 315                 320 
Ala Ser Ala Phe Thr Leu Leu Leu Ala Arg Glu Met Gly Asp Gln Gln 
                325                 330                 335     
Leu Phe Asp Gln Leu Leu Asn His Leu Glu Pro Pro Ala Lys Pro Ser 
            340                 345                 350         
Ile Val Ser Ala Ser Leu Arg Tyr Glu His Pro Gly Ser Leu Leu Phe 
        355                 360                 365             
Asp Glu Leu Leu Phe Leu Ala Lys Val His Ala Gly Phe Gly Ala Leu 
    370                 375                 380                 
Leu Arg Met Pro Pro Pro Ala Ala Lys Leu Ala Gly Lys 
385                 390                 395         

<210> 2
<211> 1218
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..1218
<223> /mol_type="unassigned DNA"
      /note="E. coli codon optimized sequence coding for linalool
      dehydratase-isomerase from Castellaniella defragrans"
      /organism="Artificial Sequence"

<220> 
<221> CDS
<222> 1..1215
<223> /transl_table=1

<400> 2
atg cac cat cat cat cat cac atg cgt ttt acc ctg aaa acc acc gca      48
Met His His His His His His Met Arg Phe Thr Leu Lys Thr Thr Ala 
1               5                   10                  15      

att gtg agc gca gca gca ctg ctg gca ggt ttt ggt cct ccg cct cgt      96
Ile Val Ser Ala Ala Ala Leu Leu Ala Gly Phe Gly Pro Pro Pro Arg 
            20                  25                  30          

gca gca gaa ctg cct ccg ggt cgt ctg gca acc acc gaa gat tat ttt     144
Ala Ala Glu Leu Pro Pro Gly Arg Leu Ala Thr Thr Glu Asp Tyr Phe 
        35                  40                  45              

gca cag cag gca aaa cag gca gtt aca ccg gat gtt atg gca cag ctg     192
Ala Gln Gln Ala Lys Gln Ala Val Thr Pro Asp Val Met Ala Gln Leu 
    50                  55                  60                  

gca tat atg aac tat att gat ttt atc agc ccg ttt tat agc cgt ggc     240
Ala Tyr Met Asn Tyr Ile Asp Phe Ile Ser Pro Phe Tyr Ser Arg Gly 
65                  70                  75                  80  

tgt agc ttt gaa gca tgg gaa ctg aaa cat aca ccg cag cgt gtt atc     288
Cys Ser Phe Glu Ala Trp Glu Leu Lys His Thr Pro Gln Arg Val Ile 
                85                  90                  95      

aaa tat agc att gcc ttt tat gcc tat ggt ctg gca agc gtt gca ctg     336
Lys Tyr Ser Ile Ala Phe Tyr Ala Tyr Gly Leu Ala Ser Val Ala Leu 
            100                 105                 110         

att gat ccg aaa ctg cgt gca ctg gca ggt cat gat ctg gat att gca     384
Ile Asp Pro Lys Leu Arg Ala Leu Ala Gly His Asp Leu Asp Ile Ala 
        115                 120                 125             

gtt agc aaa atg aaa tgc aaa cgt gtt tgg ggt gat tgg gaa gaa gat     432
Val Ser Lys Met Lys Cys Lys Arg Val Trp Gly Asp Trp Glu Glu Asp 
    130                 135                 140                 

ggt ttt ggc acc gat ccg att gaa aaa gaa aac att atg tat aaa ggc     480
Gly Phe Gly Thr Asp Pro Ile Glu Lys Glu Asn Ile Met Tyr Lys Gly 
145                 150                 155                 160 

cat ctg aat ctg atg tat ggt ctg tat cag ctg gtt acc ggt agc cgt     528
His Leu Asn Leu Met Tyr Gly Leu Tyr Gln Leu Val Thr Gly Ser Arg 
                165                 170                 175     

cgt tat gaa gca gaa cat gca cat ctg acc cgt att att cat gat gaa     576
Arg Tyr Glu Ala Glu His Ala His Leu Thr Arg Ile Ile His Asp Glu 
            180                 185                 190         

att gca gca aat ccg ttt gcc ggt att gtt tgt gaa ccg gat aac tat     624
Ile Ala Ala Asn Pro Phe Ala Gly Ile Val Cys Glu Pro Asp Asn Tyr 
        195                 200                 205             

ttt gtg cag tgt aat agc gtt gcc tat ctg agc ctg tgg gtt tat gat     672
Phe Val Gln Cys Asn Ser Val Ala Tyr Leu Ser Leu Trp Val Tyr Asp 
    210                 215                 220                 

cgt ctg cat ggc acc gat tat cgt gca gca acc cgt gca tgg ctg gat     720
Arg Leu His Gly Thr Asp Tyr Arg Ala Ala Thr Arg Ala Trp Leu Asp 
225                 230                 235                 240 

ttt att cag aaa gat ctg att gat cct gaa cgc ggt gcc ttt tat ctg     768
Phe Ile Gln Lys Asp Leu Ile Asp Pro Glu Arg Gly Ala Phe Tyr Leu 
                245                 250                 255     

agc tat cat ccg gaa agt ggt gca gtt aaa ccg tgg att agc gca tat     816
Ser Tyr His Pro Glu Ser Gly Ala Val Lys Pro Trp Ile Ser Ala Tyr 
            260                 265                 270         

acc acc gca tgg acc ctg gca atg gtt cat ggt atg gac cct gca ttt     864
Thr Thr Ala Trp Thr Leu Ala Met Val His Gly Met Asp Pro Ala Phe 
        275                 280                 285             

agc gaa cgt tat tat ccg cgt ttt aaa cag acc ttt gtg gaa gtg tat     912
Ser Glu Arg Tyr Tyr Pro Arg Phe Lys Gln Thr Phe Val Glu Val Tyr 
    290                 295                 300                 

gat gaa ggt cgt aaa gca cgt gtt cgt gaa acc gca ggc acc gat gat     960
Asp Glu Gly Arg Lys Ala Arg Val Arg Glu Thr Ala Gly Thr Asp Asp 
305                 310                 315                 320 

gca gat ggt ggt gtt ggt ctg gcc agc gca ttt acc ctg ctg ctg gca    1008
Ala Asp Gly Gly Val Gly Leu Ala Ser Ala Phe Thr Leu Leu Leu Ala 
                325                 330                 335     

cgt gaa atg ggt gat cag caa ctg ttc gat cag ctg ctg aat cat ctg    1056
Arg Glu Met Gly Asp Gln Gln Leu Phe Asp Gln Leu Leu Asn His Leu 
            340                 345                 350         

gaa cct ccg gca aaa ccg agc att gtt agc gcc agc ctg cgt tat gaa    1104
Glu Pro Pro Ala Lys Pro Ser Ile Val Ser Ala Ser Leu Arg Tyr Glu 
        355                 360                 365             

cat ccg ggt agc ctg ctg ttt gat gaa ctg ctg ttt ctg gca aaa gtt    1152
His Pro Gly Ser Leu Leu Phe Asp Glu Leu Leu Phe Leu Ala Lys Val 
    370                 375                 380                 

cat gcc ggt ttt ggt gcc ctg ctg cgt atg cct cct ccg gca gca aaa    1200
His Ala Gly Phe Gly Ala Leu Leu Arg Met Pro Pro Pro Ala Ala Lys 
385                 390                 395                 400 

ctg gca ggt aag taa taa                                            1218
Leu Ala Gly Lys        
                       

<210> 3
<211> 404
<212> PRT
<213> Artificial Sequence

<220> 
<223> [CDS]:1..1215 from SEQ ID NO 2

<400> 3
Met His His His His His His Met Arg Phe Thr Leu Lys Thr Thr Ala 
1               5                   10                  15      
Ile Val Ser Ala Ala Ala Leu Leu Ala Gly Phe Gly Pro Pro Pro Arg 
            20                  25                  30          
Ala Ala Glu Leu Pro Pro Gly Arg Leu Ala Thr Thr Glu Asp Tyr Phe 
        35                  40                  45              
Ala Gln Gln Ala Lys Gln Ala Val Thr Pro Asp Val Met Ala Gln Leu 
    50                  55                  60                  
Ala Tyr Met Asn Tyr Ile Asp Phe Ile Ser Pro Phe Tyr Ser Arg Gly 
65                  70                  75                  80  
Cys Ser Phe Glu Ala Trp Glu Leu Lys His Thr Pro Gln Arg Val Ile 
                85                  90                  95      
Lys Tyr Ser Ile Ala Phe Tyr Ala Tyr Gly Leu Ala Ser Val Ala Leu 
            100                 105                 110         
Ile Asp Pro Lys Leu Arg Ala Leu Ala Gly His Asp Leu Asp Ile Ala 
        115                 120                 125             
Val Ser Lys Met Lys Cys Lys Arg Val Trp Gly Asp Trp Glu Glu Asp 
    130                 135                 140                 
Gly Phe Gly Thr Asp Pro Ile Glu Lys Glu Asn Ile Met Tyr Lys Gly 
145                 150                 155                 160 
His Leu Asn Leu Met Tyr Gly Leu Tyr Gln Leu Val Thr Gly Ser Arg 
                165                 170                 175     
Arg Tyr Glu Ala Glu His Ala His Leu Thr Arg Ile Ile His Asp Glu 
            180                 185                 190         
Ile Ala Ala Asn Pro Phe Ala Gly Ile Val Cys Glu Pro Asp Asn Tyr 
        195                 200                 205             
Phe Val Gln Cys Asn Ser Val Ala Tyr Leu Ser Leu Trp Val Tyr Asp 
    210                 215                 220                 
Arg Leu His Gly Thr Asp Tyr Arg Ala Ala Thr Arg Ala Trp Leu Asp 
225                 230                 235                 240 
Phe Ile Gln Lys Asp Leu Ile Asp Pro Glu Arg Gly Ala Phe Tyr Leu 
                245                 250                 255     
Ser Tyr His Pro Glu Ser Gly Ala Val Lys Pro Trp Ile Ser Ala Tyr 
            260                 265                 270         
Thr Thr Ala Trp Thr Leu Ala Met Val His Gly Met Asp Pro Ala Phe 
        275                 280                 285             
Ser Glu Arg Tyr Tyr Pro Arg Phe Lys Gln Thr Phe Val Glu Val Tyr 
    290                 295                 300                 
Asp Glu Gly Arg Lys Ala Arg Val Arg Glu Thr Ala Gly Thr Asp Asp 
305                 310                 315                 320 
Ala Asp Gly Gly Val Gly Leu Ala Ser Ala Phe Thr Leu Leu Leu Ala 
                325                 330                 335     
Arg Glu Met Gly Asp Gln Gln Leu Phe Asp Gln Leu Leu Asn His Leu 
            340                 345                 350         
Glu Pro Pro Ala Lys Pro Ser Ile Val Ser Ala Ser Leu Arg Tyr Glu 
        355                 360                 365             
His Pro Gly Ser Leu Leu Phe Asp Glu Leu Leu Phe Leu Ala Lys Val 
    370                 375                 380                 
His Ala Gly Phe Gly Ala Leu Leu Arg Met Pro Pro Pro Ala Ala Lys 
385                 390                 395                 400 
Leu Ala Gly Lys 
                

<210> 4
<211> 1242
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..1242
<223> /mol_type="unassigned DNA"
      /note="E. coli codon optimized sequence coding for linalool
      dehydratase-isomerase from Castellaniella defragrans variant
      V195F-G132A"
      /organism="Artificial Sequence"

<220> 
<221> CDS
<222> 1..1236
<223> /transl_table=1

<400> 4
atg cat cat cat cat cat cac atc aca agt ttg tac aaa aaa gca ggc      48
Met His His His His His His Ile Thr Ser Leu Tyr Lys Lys Ala Gly 
1               5                   10                  15      

tgt ttt acc ctg aaa acc acc gca att gtg agc gca gca gca ctg ctg      96
Cys Phe Thr Leu Lys Thr Thr Ala Ile Val Ser Ala Ala Ala Leu Leu 
            20                  25                  30          

gca ggt ttt ggt cct ccg cct cgt gca gca gaa ctg cct ccg ggt cgt     144
Ala Gly Phe Gly Pro Pro Pro Arg Ala Ala Glu Leu Pro Pro Gly Arg 
        35                  40                  45              

ctg gca acc acc gaa gat tat ttt gca cag cag gca aaa cag gca gtt     192
Leu Ala Thr Thr Glu Asp Tyr Phe Ala Gln Gln Ala Lys Gln Ala Val 
    50                  55                  60                  

aca ccg gat gtt atg gca cag ctg gca tat atg aac tat att gat ttt     240
Thr Pro Asp Val Met Ala Gln Leu Ala Tyr Met Asn Tyr Ile Asp Phe 
65                  70                  75                  80  

atc agc ccg ttt tat agc cgt ggc tgt agc ttt gaa gca tgg gaa ctg     288
Ile Ser Pro Phe Tyr Ser Arg Gly Cys Ser Phe Glu Ala Trp Glu Leu 
                85                  90                  95      

aaa cat aca ccg cag cgt gtt atc aaa tat agc att gcc ttt tat gcc     336
Lys His Thr Pro Gln Arg Val Ile Lys Tyr Ser Ile Ala Phe Tyr Ala 
            100                 105                 110         

tat ggt ctg gca agc gtt gca ctg att gat ccg aaa ctg cgt gca ctg     384
Tyr Gly Leu Ala Ser Val Ala Leu Ile Asp Pro Lys Leu Arg Ala Leu 
        115                 120                 125             

gca ggt cat gat ctg gat att gca gtt agc aaa atg aaa tgc aaa cgt     432
Ala Gly His Asp Leu Asp Ile Ala Val Ser Lys Met Lys Cys Lys Arg 
    130                 135                 140                 

gtt tgg gcg gat tgg gaa gaa gat ggt ttt ggc acc gat ccg att gaa     480
Val Trp Ala Asp Trp Glu Glu Asp Gly Phe Gly Thr Asp Pro Ile Glu 
145                 150                 155                 160 

aaa gaa aac att atg tat aaa ggc cat ctg aat ctg atg tat ggt ctg     528
Lys Glu Asn Ile Met Tyr Lys Gly His Leu Asn Leu Met Tyr Gly Leu 
                165                 170                 175     

tat cag ctg gtt acc ggt agc cgt cgt tat gaa gca gaa cat gca cat     576
Tyr Gln Leu Val Thr Gly Ser Arg Arg Tyr Glu Ala Glu His Ala His 
            180                 185                 190         

ctg acc cgt att att cat gat gaa att gca gca aat ccg ttt gcc ggt     624
Leu Thr Arg Ile Ile His Asp Glu Ile Ala Ala Asn Pro Phe Ala Gly 
        195                 200                 205             

att ttt tgt gaa ccg gat aac tat ttt gtg cag tgt aat agc gtt gcc     672
Ile Phe Cys Glu Pro Asp Asn Tyr Phe Val Gln Cys Asn Ser Val Ala 
    210                 215                 220                 

tat ctg agc ctg tgg gtt tat gat cgt ctg cat ggc acc gat tat cgt     720
Tyr Leu Ser Leu Trp Val Tyr Asp Arg Leu His Gly Thr Asp Tyr Arg 
225                 230                 235                 240 

gca gca acc cgt gca tgg ctg gat ttt att cag aaa gat ctg att gat     768
Ala Ala Thr Arg Ala Trp Leu Asp Phe Ile Gln Lys Asp Leu Ile Asp 
                245                 250                 255     

cct gaa cgc ggt gcc ttt tat ctg agc tat cat ccg gaa agt ggt gca     816
Pro Glu Arg Gly Ala Phe Tyr Leu Ser Tyr His Pro Glu Ser Gly Ala 
            260                 265                 270         

gtt aaa ccg tgg att agc gca tat acc acc gca tgg acc ctg gca atg     864
Val Lys Pro Trp Ile Ser Ala Tyr Thr Thr Ala Trp Thr Leu Ala Met 
        275                 280                 285             

gtt cat ggt atg gac cct gca ttt agc gaa cgt tat tat ccg cgt ttt     912
Val His Gly Met Asp Pro Ala Phe Ser Glu Arg Tyr Tyr Pro Arg Phe 
    290                 295                 300                 

aaa cag acc ttt gtg gaa gtg tat gat gaa ggt cgt aaa gca cgt gtt     960
Lys Gln Thr Phe Val Glu Val Tyr Asp Glu Gly Arg Lys Ala Arg Val 
305                 310                 315                 320 

cgt gaa acc gca ggc acc gat gat gca gat ggt ggt gtt ggt ctg gcc    1008
Arg Glu Thr Ala Gly Thr Asp Asp Ala Asp Gly Gly Val Gly Leu Ala 
                325                 330                 335     

agc gca ttt acc ctg ctg ctg gca cgt gaa atg ggt gat cag caa ctg    1056
Ser Ala Phe Thr Leu Leu Leu Ala Arg Glu Met Gly Asp Gln Gln Leu 
            340                 345                 350         

ttc gat cag ctg ctg aat cat ctg gaa cct ccg gca aaa ccg agc att    1104
Phe Asp Gln Leu Leu Asn His Leu Glu Pro Pro Ala Lys Pro Ser Ile 
        355                 360                 365             

gtt agc gcc agc ctg cgt tat gaa cat ccg ggt agc ctg ctg ttt gat    1152
Val Ser Ala Ser Leu Arg Tyr Glu His Pro Gly Ser Leu Leu Phe Asp 
    370                 375                 380                 

gaa ctg ctg ttt ctg gca aaa gtt cat gcc ggt ttt ggt gcc ctg ctg    1200
Glu Leu Leu Phe Leu Ala Lys Val His Ala Gly Phe Gly Ala Leu Leu 
385                 390                 395                 400 

cgt atg cct cct ccg gca gca aaa ctg gca ggt aag taataa             1242
Arg Met Pro Pro Pro Ala Ala Lys Leu Ala Gly Lys       
                405                 410               

<210> 5
<211> 412
<212> PRT
<213> Artificial Sequence

<220> 
<223> [CDS]:1..1236 from SEQ ID NO 4

<400> 5
Met His His His His His His Ile Thr Ser Leu Tyr Lys Lys Ala Gly 
1               5                   10                  15      
Cys Phe Thr Leu Lys Thr Thr Ala Ile Val Ser Ala Ala Ala Leu Leu 
            20                  25                  30          
Ala Gly Phe Gly Pro Pro Pro Arg Ala Ala Glu Leu Pro Pro Gly Arg 
        35                  40                  45              
Leu Ala Thr Thr Glu Asp Tyr Phe Ala Gln Gln Ala Lys Gln Ala Val 
    50                  55                  60                  
Thr Pro Asp Val Met Ala Gln Leu Ala Tyr Met Asn Tyr Ile Asp Phe 
65                  70                  75                  80  
Ile Ser Pro Phe Tyr Ser Arg Gly Cys Ser Phe Glu Ala Trp Glu Leu 
                85                  90                  95      
Lys His Thr Pro Gln Arg Val Ile Lys Tyr Ser Ile Ala Phe Tyr Ala 
            100                 105                 110         
Tyr Gly Leu Ala Ser Val Ala Leu Ile Asp Pro Lys Leu Arg Ala Leu 
        115                 120                 125             
Ala Gly His Asp Leu Asp Ile Ala Val Ser Lys Met Lys Cys Lys Arg 
    130                 135                 140                 
Val Trp Ala Asp Trp Glu Glu Asp Gly Phe Gly Thr Asp Pro Ile Glu 
145                 150                 155                 160 
Lys Glu Asn Ile Met Tyr Lys Gly His Leu Asn Leu Met Tyr Gly Leu 
                165                 170                 175     
Tyr Gln Leu Val Thr Gly Ser Arg Arg Tyr Glu Ala Glu His Ala His 
            180                 185                 190         
Leu Thr Arg Ile Ile His Asp Glu Ile Ala Ala Asn Pro Phe Ala Gly 
        195                 200                 205             
Ile Phe Cys Glu Pro Asp Asn Tyr Phe Val Gln Cys Asn Ser Val Ala 
    210                 215                 220                 
Tyr Leu Ser Leu Trp Val Tyr Asp Arg Leu His Gly Thr Asp Tyr Arg 
225                 230                 235                 240 
Ala Ala Thr Arg Ala Trp Leu Asp Phe Ile Gln Lys Asp Leu Ile Asp 
                245                 250                 255     
Pro Glu Arg Gly Ala Phe Tyr Leu Ser Tyr His Pro Glu Ser Gly Ala 
            260                 265                 270         
Val Lys Pro Trp Ile Ser Ala Tyr Thr Thr Ala Trp Thr Leu Ala Met 
        275                 280                 285             
Val His Gly Met Asp Pro Ala Phe Ser Glu Arg Tyr Tyr Pro Arg Phe 
    290                 295                 300                 
Lys Gln Thr Phe Val Glu Val Tyr Asp Glu Gly Arg Lys Ala Arg Val 
305                 310                 315                 320 
Arg Glu Thr Ala Gly Thr Asp Asp Ala Asp Gly Gly Val Gly Leu Ala 
                325                 330                 335     
Ser Ala Phe Thr Leu Leu Leu Ala Arg Glu Met Gly Asp Gln Gln Leu 
            340                 345                 350         
Phe Asp Gln Leu Leu Asn His Leu Glu Pro Pro Ala Lys Pro Ser Ile 
        355                 360                 365             
Val Ser Ala Ser Leu Arg Tyr Glu His Pro Gly Ser Leu Leu Phe Asp 
    370                 375                 380                 
Glu Leu Leu Phe Leu Ala Lys Val His Ala Gly Phe Gly Ala Leu Leu 
385                 390                 395                 400 
Arg Met Pro Pro Pro Ala Ala Lys Leu Ala Gly Lys 
                405                 410         

<210> 6
<211> 1242
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..1242
<223> /mol_type="unassigned DNA"
      /note="E. coli codon optimized sequence coding for linalool
      dehydratase-isomerase from Castellaniella defragrans variant
      V195F"
      /organism="Artificial Sequence"

<220> 
<221> CDS
<222> 1..1236
<223> /transl_table=1

<400> 6
atg cat cat cat cat cat cac atc aca agt ttg tac aaa aaa gca ggc      48
Met His His His His His His Ile Thr Ser Leu Tyr Lys Lys Ala Gly 
1               5                   10                  15      

tgt ttt acc ctg aaa acc acc gca att gtg agc gca gca gca ctg ctg      96
Cys Phe Thr Leu Lys Thr Thr Ala Ile Val Ser Ala Ala Ala Leu Leu 
            20                  25                  30          

gca ggt ttt ggt cct ccg cct cgt gca gca gaa ctg cct ccg ggt cgt     144
Ala Gly Phe Gly Pro Pro Pro Arg Ala Ala Glu Leu Pro Pro Gly Arg 
        35                  40                  45              

ctg gca acc acc gaa gat tat ttt gca cag cag gca aaa cag gca gtt     192
Leu Ala Thr Thr Glu Asp Tyr Phe Ala Gln Gln Ala Lys Gln Ala Val 
    50                  55                  60                  

aca ccg gat gtt atg gca cag ctg gca tat atg aac tat att gat ttt     240
Thr Pro Asp Val Met Ala Gln Leu Ala Tyr Met Asn Tyr Ile Asp Phe 
65                  70                  75                  80  

atc agc ccg ttt tat agc cgt ggc tgt agc ttt gaa gca tgg gaa ctg     288
Ile Ser Pro Phe Tyr Ser Arg Gly Cys Ser Phe Glu Ala Trp Glu Leu 
                85                  90                  95      

aaa cat aca ccg cag cgt gtt atc aaa tat agc att gcc ttt tat gcc     336
Lys His Thr Pro Gln Arg Val Ile Lys Tyr Ser Ile Ala Phe Tyr Ala 
            100                 105                 110         

tat ggt ctg gca agc gtt gca ctg att gat ccg aaa ctg cgt gca ctg     384
Tyr Gly Leu Ala Ser Val Ala Leu Ile Asp Pro Lys Leu Arg Ala Leu 
        115                 120                 125             

gca ggt cat gat ctg gat att gca gtt agc aaa atg aaa tgc aaa cgt     432
Ala Gly His Asp Leu Asp Ile Ala Val Ser Lys Met Lys Cys Lys Arg 
    130                 135                 140                 

gtt tgg ggt gat tgg gaa gaa gat ggt ttt ggc acc gat ccg att gaa     480
Val Trp Gly Asp Trp Glu Glu Asp Gly Phe Gly Thr Asp Pro Ile Glu 
145                 150                 155                 160 

aaa gaa aac att atg tat aaa ggc cat ctg aat ctg atg tat ggt ctg     528
Lys Glu Asn Ile Met Tyr Lys Gly His Leu Asn Leu Met Tyr Gly Leu 
                165                 170                 175     

tat cag ctg gtt acc ggt agc cgt cgt tat gaa gca gaa cat gca cat     576
Tyr Gln Leu Val Thr Gly Ser Arg Arg Tyr Glu Ala Glu His Ala His 
            180                 185                 190         

ctg acc cgt att att cat gat gaa att gca gca aat ccg ttt gcc ggt     624
Leu Thr Arg Ile Ile His Asp Glu Ile Ala Ala Asn Pro Phe Ala Gly 
        195                 200                 205             

att ttt tgt gaa ccg gat aac tat ttt gtg cag tgt aat agc gtt gcc     672
Ile Phe Cys Glu Pro Asp Asn Tyr Phe Val Gln Cys Asn Ser Val Ala 
    210                 215                 220                 

tat ctg agc ctg tgg gtt tat gat cgt ctg cat ggc acc gat tat cgt     720
Tyr Leu Ser Leu Trp Val Tyr Asp Arg Leu His Gly Thr Asp Tyr Arg 
225                 230                 235                 240 

gca gca acc cgt gca tgg ctg gat ttt att cag aaa gat ctg att gat     768
Ala Ala Thr Arg Ala Trp Leu Asp Phe Ile Gln Lys Asp Leu Ile Asp 
                245                 250                 255     

cct gaa cgc ggt gcc ttt tat ctg agc tat cat ccg gaa agt ggt gca     816
Pro Glu Arg Gly Ala Phe Tyr Leu Ser Tyr His Pro Glu Ser Gly Ala 
            260                 265                 270         

gtt aaa ccg tgg att agc gca tat acc acc gca tgg acc ctg gca atg     864
Val Lys Pro Trp Ile Ser Ala Tyr Thr Thr Ala Trp Thr Leu Ala Met 
        275                 280                 285             

gtt cat ggt atg gac cct gca ttt agc gaa cgt tat tat ccg cgt ttt     912
Val His Gly Met Asp Pro Ala Phe Ser Glu Arg Tyr Tyr Pro Arg Phe 
    290                 295                 300                 

aaa cag acc ttt gtg gaa gtg tat gat gaa ggt cgt aaa gca cgt gtt     960
Lys Gln Thr Phe Val Glu Val Tyr Asp Glu Gly Arg Lys Ala Arg Val 
305                 310                 315                 320 

cgt gaa acc gca ggc acc gat gat gca gat ggt ggt gtt ggt ctg gcc    1008
Arg Glu Thr Ala Gly Thr Asp Asp Ala Asp Gly Gly Val Gly Leu Ala 
                325                 330                 335     

agc gca ttt acc ctg ctg ctg gca cgt gaa atg ggt gat cag caa ctg    1056
Ser Ala Phe Thr Leu Leu Leu Ala Arg Glu Met Gly Asp Gln Gln Leu 
            340                 345                 350         

ttc gat cag ctg ctg aat cat ctg gaa cct ccg gca aaa ccg agc att    1104
Phe Asp Gln Leu Leu Asn His Leu Glu Pro Pro Ala Lys Pro Ser Ile 
        355                 360                 365             

gtt agc gcc agc ctg cgt tat gaa cat ccg ggt agc ctg ctg ttt gat    1152
Val Ser Ala Ser Leu Arg Tyr Glu His Pro Gly Ser Leu Leu Phe Asp 
    370                 375                 380                 

gaa ctg ctg ttt ctg gca aaa gtt cat gcc ggt ttt ggt gcc ctg ctg    1200
Glu Leu Leu Phe Leu Ala Lys Val His Ala Gly Phe Gly Ala Leu Leu 
385                 390                 395                 400 

cgt atg cct cct ccg gca gca aaa ctg gca ggt aag taataa             1242
Arg Met Pro Pro Pro Ala Ala Lys Leu Ala Gly Lys       
                405                 410               

<210> 7
<211> 412
<212> PRT
<213> Artificial Sequence

<220> 
<223> [CDS]:1..1236 from SEQ ID NO 6

<400> 7
Met His His His His His His Ile Thr Ser Leu Tyr Lys Lys Ala Gly 
1               5                   10                  15      
Cys Phe Thr Leu Lys Thr Thr Ala Ile Val Ser Ala Ala Ala Leu Leu 
            20                  25                  30          
Ala Gly Phe Gly Pro Pro Pro Arg Ala Ala Glu Leu Pro Pro Gly Arg 
        35                  40                  45              
Leu Ala Thr Thr Glu Asp Tyr Phe Ala Gln Gln Ala Lys Gln Ala Val 
    50                  55                  60                  
Thr Pro Asp Val Met Ala Gln Leu Ala Tyr Met Asn Tyr Ile Asp Phe 
65                  70                  75                  80  
Ile Ser Pro Phe Tyr Ser Arg Gly Cys Ser Phe Glu Ala Trp Glu Leu 
                85                  90                  95      
Lys His Thr Pro Gln Arg Val Ile Lys Tyr Ser Ile Ala Phe Tyr Ala 
            100                 105                 110         
Tyr Gly Leu Ala Ser Val Ala Leu Ile Asp Pro Lys Leu Arg Ala Leu 
        115                 120                 125             
Ala Gly His Asp Leu Asp Ile Ala Val Ser Lys Met Lys Cys Lys Arg 
    130                 135                 140                 
Val Trp Gly Asp Trp Glu Glu Asp Gly Phe Gly Thr Asp Pro Ile Glu 
145                 150                 155                 160 
Lys Glu Asn Ile Met Tyr Lys Gly His Leu Asn Leu Met Tyr Gly Leu 
                165                 170                 175     
Tyr Gln Leu Val Thr Gly Ser Arg Arg Tyr Glu Ala Glu His Ala His 
            180                 185                 190         
Leu Thr Arg Ile Ile His Asp Glu Ile Ala Ala Asn Pro Phe Ala Gly 
        195                 200                 205             
Ile Phe Cys Glu Pro Asp Asn Tyr Phe Val Gln Cys Asn Ser Val Ala 
    210                 215                 220                 
Tyr Leu Ser Leu Trp Val Tyr Asp Arg Leu His Gly Thr Asp Tyr Arg 
225                 230                 235                 240 
Ala Ala Thr Arg Ala Trp Leu Asp Phe Ile Gln Lys Asp Leu Ile Asp 
                245                 250                 255     
Pro Glu Arg Gly Ala Phe Tyr Leu Ser Tyr His Pro Glu Ser Gly Ala 
            260                 265                 270         
Val Lys Pro Trp Ile Ser Ala Tyr Thr Thr Ala Trp Thr Leu Ala Met 
        275                 280                 285             
Val His Gly Met Asp Pro Ala Phe Ser Glu Arg Tyr Tyr Pro Arg Phe 
    290                 295                 300                 
Lys Gln Thr Phe Val Glu Val Tyr Asp Glu Gly Arg Lys Ala Arg Val 
305                 310                 315                 320 
Arg Glu Thr Ala Gly Thr Asp Asp Ala Asp Gly Gly Val Gly Leu Ala 
                325                 330                 335     
Ser Ala Phe Thr Leu Leu Leu Ala Arg Glu Met Gly Asp Gln Gln Leu 
            340                 345                 350         
Phe Asp Gln Leu Leu Asn His Leu Glu Pro Pro Ala Lys Pro Ser Ile 
        355                 360                 365             
Val Ser Ala Ser Leu Arg Tyr Glu His Pro Gly Ser Leu Leu Phe Asp 
    370                 375                 380                 
Glu Leu Leu Phe Leu Ala Lys Val His Ala Gly Phe Gly Ala Leu Leu 
385                 390                 395                 400 
Arg Met Pro Pro Pro Ala Ala Lys Leu Ala Gly Lys 
                405                 410         


<210> 8
<211> 1242
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..1242
<223> /mol_type="unassigned DNA"
      /note="E. coli codon optimized sequence coding for linalool
      dehydratase-isomerase from Castellaniella defragrans wild type 
      sequence encoded by pET300NT/DEST"
      /organism="Artificial Sequence"

<220> 
<221> CDS
<222> 1..1236
<223> /transl_table=1

<400> 8
atg cat cat cat cat cat cac atc aca agt ttg tac aaa aaa gca ggc      48
Met His His His His His His Ile Thr Ser Leu Tyr Lys Lys Ala Gly 
1               5                   10                  15      

tgt ttt acc ctg aaa acc acc gca att gtg agc gca gca gca ctg ctg      96
Cys Phe Thr Leu Lys Thr Thr Ala Ile Val Ser Ala Ala Ala Leu Leu 
            20                  25                  30          

gca ggt ttt ggt cct ccg cct cgt gca gca gaa ctg cct ccg ggt cgt     144
Ala Gly Phe Gly Pro Pro Pro Arg Ala Ala Glu Leu Pro Pro Gly Arg 
        35                  40                  45              

ctg gca acc acc gaa gat tat ttt gca cag cag gca aaa cag gca gtt     192
Leu Ala Thr Thr Glu Asp Tyr Phe Ala Gln Gln Ala Lys Gln Ala Val 
    50                  55                  60                  

aca ccg gat gtt atg gca cag ctg gca tat atg aac tat att gat ttt     240
Thr Pro Asp Val Met Ala Gln Leu Ala Tyr Met Asn Tyr Ile Asp Phe 
65                  70                  75                  80  

atc agc ccg ttt tat agc cgt ggc tgt agc ttt gaa gca tgg gaa ctg     288
Ile Ser Pro Phe Tyr Ser Arg Gly Cys Ser Phe Glu Ala Trp Glu Leu 
                85                  90                  95      

aaa cat aca ccg cag cgt gtt atc aaa tat agc att gcc ttt tat gcc     336
Lys His Thr Pro Gln Arg Val Ile Lys Tyr Ser Ile Ala Phe Tyr Ala 
            100                 105                 110         

tat ggt ctg gca agc gtt gca ctg att gat ccg aaa ctg cgt gca ctg     384
Tyr Gly Leu Ala Ser Val Ala Leu Ile Asp Pro Lys Leu Arg Ala Leu 
        115                 120                 125             

gca ggt cat gat ctg gat att gca gtt agc aaa atg aaa tgc aaa cgt     432
Ala Gly His Asp Leu Asp Ile Ala Val Ser Lys Met Lys Cys Lys Arg 
    130                 135                 140                 

gtt tgg ggt gat tgg gaa gaa gat ggt ttt ggc acc gat ccg att gaa     480
Val Trp Gly Asp Trp Glu Glu Asp Gly Phe Gly Thr Asp Pro Ile Glu 
145                 150                 155                 160 

aaa gaa aac att atg tat aaa ggc cat ctg aat ctg atg tat ggt ctg     528
Lys Glu Asn Ile Met Tyr Lys Gly His Leu Asn Leu Met Tyr Gly Leu 
                165                 170                 175     

tat cag ctg gtt acc ggt agc cgt cgt tat gaa gca gaa cat gca cat     576
Tyr Gln Leu Val Thr Gly Ser Arg Arg Tyr Glu Ala Glu His Ala His 
            180                 185                 190         

ctg acc cgt att att cat gat gaa att gca gca aat ccg ttt gcc ggt     624
Leu Thr Arg Ile Ile His Asp Glu Ile Ala Ala Asn Pro Phe Ala Gly 
        195                 200                 205             

att gtt tgt gaa ccg gat aac tat ttt gtg cag tgt aat agc gtt gcc     672
Ile Val Cys Glu Pro Asp Asn Tyr Phe Val Gln Cys Asn Ser Val Ala 
    210                 215                 220                 

tat ctg agc ctg tgg gtt tat gat cgt ctg cat ggc acc gat tat cgt     720
Tyr Leu Ser Leu Trp Val Tyr Asp Arg Leu His Gly Thr Asp Tyr Arg 
225                 230                 235                 240 

gca gca acc cgt gca tgg ctg gat ttt att cag aaa gat ctg att gat     768
Ala Ala Thr Arg Ala Trp Leu Asp Phe Ile Gln Lys Asp Leu Ile Asp 
                245                 250                 255     

cct gaa cgc ggt gcc ttt tat ctg agc tat cat ccg gaa agt ggt gca     816
Pro Glu Arg Gly Ala Phe Tyr Leu Ser Tyr His Pro Glu Ser Gly Ala 
            260                 265                 270         

gtt aaa ccg tgg att agc gca tat acc acc gca tgg acc ctg gca atg     864
Val Lys Pro Trp Ile Ser Ala Tyr Thr Thr Ala Trp Thr Leu Ala Met 
        275                 280                 285             

gtt cat ggt atg gac cct gca ttt agc gaa cgt tat tat ccg cgt ttt     912
Val His Gly Met Asp Pro Ala Phe Ser Glu Arg Tyr Tyr Pro Arg Phe 
    290                 295                 300                 

aaa cag acc ttt gtg gaa gtg tat gat gaa ggt cgt aaa gca cgt gtt     960
Lys Gln Thr Phe Val Glu Val Tyr Asp Glu Gly Arg Lys Ala Arg Val 
305                 310                 315                 320 

cgt gaa acc gca ggc acc gat gat gca gat ggt ggt gtt ggt ctg gcc    1008
Arg Glu Thr Ala Gly Thr Asp Asp Ala Asp Gly Gly Val Gly Leu Ala 
                325                 330                 335     

agc gca ttt acc ctg ctg ctg gca cgt gaa atg ggt gat cag caa ctg    1056
Ser Ala Phe Thr Leu Leu Leu Ala Arg Glu Met Gly Asp Gln Gln Leu 
            340                 345                 350         

ttc gat cag ctg ctg aat cat ctg gaa cct ccg gca aaa ccg agc att    1104
Phe Asp Gln Leu Leu Asn His Leu Glu Pro Pro Ala Lys Pro Ser Ile 
        355                 360                 365             

gtt agc gcc agc ctg cgt tat gaa cat ccg ggt agc ctg ctg ttt gat    1152
Val Ser Ala Ser Leu Arg Tyr Glu His Pro Gly Ser Leu Leu Phe Asp 
    370                 375                 380                 

gaa ctg ctg ttt ctg gca aaa gtt cat gcc ggt ttt ggt gcc ctg ctg    1200
Glu Leu Leu Phe Leu Ala Lys Val His Ala Gly Phe Gly Ala Leu Leu 
385                 390                 395                 400 

cgt atg cct cct ccg gca gca aaa ctg gca ggt aag taataa             1242
Arg Met Pro Pro Pro Ala Ala Lys Leu Ala Gly Lys       
                405                 410               


<210> 9
<211> 412
<212> PRT
<213> Artificial Sequence

<220> 
<223> [CDS]:1..1236 from SEQ ID NO 8

<400> 9
Met His His His His His His Ile Thr Ser Leu Tyr Lys Lys Ala Gly 
1               5                   10                  15      
Cys Phe Thr Leu Lys Thr Thr Ala Ile Val Ser Ala Ala Ala Leu Leu 
            20                  25                  30          
Ala Gly Phe Gly Pro Pro Pro Arg Ala Ala Glu Leu Pro Pro Gly Arg 
        35                  40                  45              
Leu Ala Thr Thr Glu Asp Tyr Phe Ala Gln Gln Ala Lys Gln Ala Val 
    50                  55                  60                  
Thr Pro Asp Val Met Ala Gln Leu Ala Tyr Met Asn Tyr Ile Asp Phe 
65                  70                  75                  80  
Ile Ser Pro Phe Tyr Ser Arg Gly Cys Ser Phe Glu Ala Trp Glu Leu 
                85                  90                  95      
Lys His Thr Pro Gln Arg Val Ile Lys Tyr Ser Ile Ala Phe Tyr Ala 
            100                 105                 110         
Tyr Gly Leu Ala Ser Val Ala Leu Ile Asp Pro Lys Leu Arg Ala Leu 
        115                 120                 125             
Ala Gly His Asp Leu Asp Ile Ala Val Ser Lys Met Lys Cys Lys Arg 
    130                 135                 140                 
Val Trp Gly Asp Trp Glu Glu Asp Gly Phe Gly Thr Asp Pro Ile Glu 
145                 150                 155                 160 
Lys Glu Asn Ile Met Tyr Lys Gly His Leu Asn Leu Met Tyr Gly Leu 
                165                 170                 175     
Tyr Gln Leu Val Thr Gly Ser Arg Arg Tyr Glu Ala Glu His Ala His 
            180                 185                 190         
Leu Thr Arg Ile Ile His Asp Glu Ile Ala Ala Asn Pro Phe Ala Gly 
        195                 200                 205             
Ile Val Cys Glu Pro Asp Asn Tyr Phe Val Gln Cys Asn Ser Val Ala 
    210                 215                 220                 
Tyr Leu Ser Leu Trp Val Tyr Asp Arg Leu His Gly Thr Asp Tyr Arg 
225                 230                 235                 240 
Ala Ala Thr Arg Ala Trp Leu Asp Phe Ile Gln Lys Asp Leu Ile Asp 
                245                 250                 255     
Pro Glu Arg Gly Ala Phe Tyr Leu Ser Tyr His Pro Glu Ser Gly Ala 
            260                 265                 270         
Val Lys Pro Trp Ile Ser Ala Tyr Thr Thr Ala Trp Thr Leu Ala Met 
        275                 280                 285             
Val His Gly Met Asp Pro Ala Phe Ser Glu Arg Tyr Tyr Pro Arg Phe 
    290                 295                 300                 
Lys Gln Thr Phe Val Glu Val Tyr Asp Glu Gly Arg Lys Ala Arg Val 
305                 310                 315                 320 
Arg Glu Thr Ala Gly Thr Asp Asp Ala Asp Gly Gly Val Gly Leu Ala 
                325                 330                 335     
Ser Ala Phe Thr Leu Leu Leu Ala Arg Glu Met Gly Asp Gln Gln Leu 
            340                 345                 350         
Phe Asp Gln Leu Leu Asn His Leu Glu Pro Pro Ala Lys Pro Ser Ile 
        355                 360                 365             
Val Ser Ala Ser Leu Arg Tyr Glu His Pro Gly Ser Leu Leu Phe Asp 
    370                 375                 380                 
Glu Leu Leu Phe Leu Ala Lys Val His Ala Gly Phe Gly Ala Leu Leu 
385                 390                 395                 400 
Arg Met Pro Pro Pro Ala Ala Lys Leu Ala Gly Lys 
                405                 410         

