                         SEQUENCE LISTING

<110>  COOPER, LAURENCE J N
       TORIKAI, HIROKI
       ZHANG, LING
       HULS, HELEN
       WANG-JOHANNING, FENG
       HURTON, LENKA
       OLIVARES, SIMON
       KRISHNAMURTHY, JANANI
 
<120>  HUMAN APPLICATION OF ENGINEERED CHIMERIC ANTIGEN RECEPTOR (CAR) 
       T-CELLS

<130>  UTFC.P1222WO

<140>  Unknown
<141>  2014-05-14

<150>  61/823,253
<151>  2013-05-14

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  711
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  fusion protein

<400>  1

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Leu Cys Glu Leu Pro His 
1               5                   10                  15      


Pro Ala Phe Leu Leu Ile Pro Asp Ile Gln Met Thr Gln Gln Thr Thr 
            20                  25                  30          


Ser Ser Leu Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg 
        35                  40                  45              


Ala Ser Ser Gln Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys 
    50                  55                  60                  


Pro Asp Gly Thr Val Lys Leu Leu Leu Ile Tyr His Thr Ser Arg Leu 
65                  70                  75                  80  


His Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Gly Ser Gly Thr 
                85                  90                  95      


Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr 
            100                 105                 110         


Tyr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr Thr Phe Gly Gly 
        115                 120                 125             


Gly Thr Lys Leu Glu Ile Ile Thr Gly Ser Thr Ser Gly Ser Gly Lys 
    130                 135                 140                 


Pro Gly Ser Gly Glu Gly Ser Thr Lys Gly Glu Glu Val Lys Leu Gln 
145                 150                 155                 160 


Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser Leu Ser Val Thr 
                165                 170                 175     


Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly Val Ser Trp 
            180                 185                 190         


Ile Arg Gln Pro Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly Val Ile 
        195                 200                 205             


Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ser Ala Leu Lys Ser Arg 
    210                 215                 220                 


Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Lys 
225                 230                 235                 240 


Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys 
                245                 250                 255     


His Tyr Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln 
            260                 265                 270         


Gly Thr Ser Val Thr Val Ser Ser Glu Glu Ser Lys Tyr Gly Pro Pro 
        275                 280                 285             


Cys Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Ser Val 
    290                 295                 300                 


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
305                 310                 315                 320 


Pro Glu Val Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro 
                325                 330                 335     


Glu Val Gln Phe Asn Trp Tyr Val Val Asp Gly Val Glu Val His Asn 
            340                 345                 350         


Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Thr Tyr Arg 
        355                 360                 365             


Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys 
    370                 375                 380                 


Glu Tyr Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile 
385                 390                 395                 400 


Glu Lys Thr Ile Ser Lys Ala Ala Lys Gly Gln Pro Arg Glu Pro Gln 
                405                 410                 415     


Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Thr Lys Asn Gln 
            420                 425                 430         


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
        435                 440                 445             


Val Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 
    450                 455                 460                 


Thr Pro Pro Val Leu Asp Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
465                 470                 475                 480 


Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Glu Gly Asn Val Phe 
                485                 490                 495     


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
            500                 505                 510         


Lys Ser Leu Ser Leu Ser Leu Gly Lys Met Phe Trp Val Leu Val Val 
        515                 520                 525             


Val Gly Gly Val Leu Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala 
    530                 535                 540                 


Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Arg Ser Arg Gly Gly His 
545                 550                 555                 560 


Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Arg 
                565                 570                 575     


Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg 
            580                 585                 590         


Ser Arg Val Lys Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln 
        595                 600                 605             


Gln Gly Gln Asn Gln Leu Tyr Asn Glu Glu Leu Asn Leu Gly Arg Arg 
    610                 615                 620                 


Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Pro Glu 
625                 630                 635                 640 


Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn 
                645                 650                 655     


Glu Leu Gln Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly 
            660                 665                 670         


Met Lys Gly Glu Arg Arg Arg Gly Gly Lys Gly His Asp Gly Leu Tyr 
        675                 680                 685             


Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Ala Leu His 
    690                 695                 700                 


Met Gln Ala Leu Pro Pro Arg 
705                 710     


<210>  2
<211>  2034
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide sequence

<400>  2
atgctgctgc tggtgaccag cctgctgctg tgtgagctgc cccaccccgc ctttctgctg       60

atccccgaca tccagatgac ccagaccacc tccagcctga gcgccagcct gggcgaccgg      120

gtgaccatca gctgccgggc cagccaggac atcagcaagt acctgaactg gtatcagcag      180

aagcccgacg gcaccgtcaa gctgctgatc taccacacca gccggctgca cagcggcgtg      240

cccagccggt ttagcggcag cggctccggc accgactaca gcctgaccat ctccaacctg      300

gagcaggagg acatcgccac ctacttttgc cagcagggca acacactgcc ctacaccttt      360

ggcggcggaa caaagctgga gatcaccggc agcacctccg gcagcggcaa gcctggcagc      420

ggcgagggca gcaccaaggg cgaggtgaag ctgcaggaga gcggccctgg cctggtggcc      480

cccagccaga gcctgagcgt gacctgtacc gtgtccggcg tgtccctgcc cgactacggc      540

gtgtcctgga tccggcagcc ccctaggaag ggcctggagt ggctgggcgt gatctggggc      600

agcgagacca cctactacaa cagcgccctg aagagccggc tgaccatcat caaggacaac      660

agcaagagcc aggtgttcct gaagatgaac agcctgcaga ccgacgacac cgccatctac      720

tactgtgcca agcactacta ctacggcggc agctacgcca tggactactg gggccagggc      780

accagcgtga ccgtgtccag cgagagcaag tacggccctc cctgcccccc ttgccctgcc      840

cccgagttcc tgggcggacc cagcgtgttc ctgttccccc ccaagcccaa ggacaccctg      900

atgatcagcc ggacccccga ggtgacctgt gtggtggtgg acgtgtccca ggaggacccc      960

gaggtccagt tcaactggta cgtggacggc gtggaggtgc acaacgccaa gaccaagccc     1020

cgggaggagc agttcaatag cacctaccgg gtggtgtccg tgctgaccgt gctgcaccag     1080

gactggctga acggcaagga atacaagtgt aaggtgtcca acaagggcct gcccagcagc     1140

atcgagaaaa ccatcagcaa ggccaagggc cagcctcggg agccccaggt gtacaccctg     1200

ccccctagcc aagaggagat gaccaagaat caggtgtccc tgacctgcct ggtgaagggc     1260

ttctacccca gcgacatcgc cgtggagtgg gagagcaacg gccagcccga gaacaactac     1320

aagaccaccc cccctgtgct ggacagcgac ggcagcttct tcctgtacag caggctgacc     1380

gtggacaaga gccggtggca ggagggcaac gtctttagct gctccgtgat gcacgaggcc     1440

ctgcacaacc actacaccca gaagagcctg tccctgagcc tgggcaagat gttctgggtg     1500

ctggtcgtgg tgggtggcgt gctggcctgc tacagcctgc tggtgacagt ggccttcatc     1560

atcttttggg tgaggagcaa gcggagcaga ggcggccaca gcgactacat gaacatgacc     1620

ccccggaggc ctggccccac ccggaagcac taccagccct acgcccctcc cagggacttc     1680

gccgcctacc ggagccgggt gaagttcagc cggagcgccg acgcccctgc ctaccagcag     1740

ggccagaacc agctgtacaa cgagctgaac ctgggccgga gggaggagta cgacgtgctg     1800

gacaagcgga gaggccggga ccctgagatg ggcggcaagc cccggagaaa gaaccctcag     1860

gagggcctgt ataacgaact gcagaaagac aagatggccg aggcctacag cgagatcggc     1920

atgaagggcg agcggcggag gggcaagggc cacgacggcc tgtaccaggg cctgagcacc     1980

gccaccaagg atacctacga cgccctgcac atgcaggccc tgccccccag atga           2034


<210>  3
<211>  4246
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide sequence

<400>  3
agttgaagtc ggaagtttac atacacttaa gttggagtca ttaaaactcg tttttcaact       60

actccacaaa tttcttgtta acaaacaata gttttggcaa gtcagttagg acatctactt      120

tgtgcatgac acaagtcatt tttccaacaa ttgtttacag acagattatt tcacttataa      180

ttcactgtat cacaattcca gtgggtcaga agtttacata cactaagttg actgtgcctt      240

taaacagctt ggaaaattcc agaaaatgat gtcatggctt tagaagcttc tgatagacta      300

attgacatca tttgagtcaa ttggaggtgt acctgtggat gtatttcaag gaattctgtg      360

gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca      420

aagcatcgag cggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt      480

tgtgtgaatc gtaactaaca tacgctctcc atcaaaacaa aacgaaacaa aacaaactag      540

caaaataggc tgtccccagt gcaagtgcag gtgccagaac atttctctat cgaaggatct      600

gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt      660

tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg      720

aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa      780

gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga      840

agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc      900

cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg      960

ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga     1020

gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg     1080

tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctacct     1140

gagatcaccg gcgaaggagg cctatcatga agatctatcg attgtacagc tagccgccac     1200

catgctgctg ctggtgacca gcctgctgct gtgtgagctg ccccaccccg cctttctgct     1260

gatccccgac atccagatga cccagaccac ctccagcctg agcgccagcc tgggcgaccg     1320

ggtgaccatc agctgccggg ccagccagga catcagcaag tacctgaact ggtatcagca     1380

gaagcccgac ggcaccgtca agctgctgat ctaccacacc agccggctgc acagcggcgt     1440

gcccagccgg tttagcggca gcggctccgg caccgactac agcctgacca tctccaacct     1500

ggagcaggag gacatcgcca cctacttttg ccagcagggc aacacactgc cctacacctt     1560

tggcggcgga acaaagctgg agatcaccgg cagcacctcc ggcagcggca agcctggcag     1620

cggcgagggc agcaccaagg gcgaggtgaa gctgcaggag agcggccctg gcctggtggc     1680

ccccagccag agcctgagcg tgacctgtac cgtgtccggc gtgtccctgc ccgactacgg     1740

cgtgtcctgg atccggcagc cccctaggaa gggcctggag tggctgggcg tgatctgggg     1800

cagcgagacc acctactaca acagcgccct gaagagccgg ctgaccatca tcaaggacaa     1860

cagcaagagc caggtgttcc tgaagatgaa cagcctgcag accgacgaca ccgccatcta     1920

ctactgtgcc aagcactact actacggcgg cagctacgcc atggactact ggggccaggg     1980

caccagcgtg accgtgtcca gcgagagcaa gtacggccct ccctgccccc cttgccctgc     2040

ccccgagttc ctgggcggac ccagcgtgtt cctgttcccc cccaagccca aggacaccct     2100

gatgatcagc cggacccccg aggtgacctg tgtggtggtg gacgtgtccc aggaggaccc     2160

cgaggtccag ttcaactggt acgtggacgg cgtggaggtg cacaacgcca agaccaagcc     2220

ccgggaggag cagttcaata gcacctaccg ggtggtgtcc gtgctgaccg tgctgcacca     2280

ggactggctg aacggcaagg aatacaagtg taaggtgtcc aacaagggcc tgcccagcag     2340

catcgagaaa accatcagca aggccaaggg ccagcctcgg gagccccagg tgtacaccct     2400

gccccctagc caagaggaga tgaccaagaa tcaggtgtcc ctgacctgcc tggtgaaggg     2460

cttctacccc agcgacatcg ccgtggagtg ggagagcaac ggccagcccg agaacaacta     2520

caagaccacc ccccctgtgc tggacagcga cggcagcttc ttcctgtaca gcaggctgac     2580

cgtggacaag agccggtggc aggagggcaa cgtctttagc tgctccgtga tgcacgaggc     2640

cctgcacaac cactacaccc agaagagcct gtccctgagc ctgggcaaga tgttctgggt     2700

gctggtcgtg gtgggtggcg tgctggcctg ctacagcctg ctggtgacag tggccttcat     2760

catcttttgg gtgaggagca agcggagcag aggcggccac agcgactaca tgaacatgac     2820

cccccggagg cctggcccca cccggaagca ctaccagccc tacgcccctc ccagggactt     2880

cgccgcctac cggagccggg tgaagttcag ccggagcgcc gacgcccctg cctaccagca     2940

gggccagaac cagctgtaca acgagctgaa cctgggccgg agggaggagt acgacgtgct     3000

ggacaagcgg agaggccggg accctgagat gggcggcaag ccccggagaa agaaccctca     3060

ggagggcctg tataacgaac tgcagaaaga caagatggcc gaggcctaca gcgagatcgg     3120

catgaagggc gagcggcgga ggggcaaggg ccacgacggc ctgtaccagg gcctgagcac     3180

cgccaccaag gatacctacg acgccctgca catgcaggcc ctgcccccca gatgactacg     3240

acccgggtga tcagcgggat ctgctgtgcc ttctagttgc cagccatctg ttgtttgccc     3300

ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa     3360

tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg     3420

gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg     3480

ctctatgggt acccaggtgc tgaagaattg acccggttcc tcctgggcca gaaagaagca     3540

ggcacatccc cttctctgtg acacaccctg tccacgcccc tggttcttag ttccagcccc     3600

actcatagga cactcatagc tcaggagggc tccgccttca atcccacccg ctaaagtact     3660

tggagcggtc tctccctccc tcatcagccc accaaaccaa acctagcctc caagagtggg     3720

aagaaattaa agcaagatag gctattaagt gcagagggag agaaaatgcc tccaacatgt     3780

gaggaagtaa tgagagaaat catagaatta tcgggccgct gcattctagt tgtggtttgt     3840

ccaaactcat caatgtatct tatcatgtct ggatcccatc acaaagctct gacctcaatc     3900

ctatagaaag gaggaatgag ccaaaattca cccaacttat tgtgggaagc ttgtggaagg     3960

ctactcgaaa tgtttgaccc aagttaaaca atttaaaggc aatgctacca aatactaatt     4020

gagtgtatgt taacttctga cccactggga atgtgatgaa agaaataaaa gctgaaatga     4080

atcattctct ctactattat tctgatattt cacattctta aaataaagtg gtgatcctaa     4140

ctgaccttaa gacagggaat ctttactcgg attaaatgtc aggaattgtg aaaaagtgag     4200

tttaaatgta tttggctaag gtgtatgtaa acttccgact tcaact                    4246


<210>  4
<211>  680
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein

<400>  4

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Met Ala Gln Val Lys Leu Gln Gln Ser Gly 
            20                  25                  30          


Pro Asp Leu Val Lys Pro Gly Ala Ser Val Lys Ile Ser Cys Lys Ala 
        35                  40                  45              


Ser Gly Tyr Ser Phe Thr Gly Tyr Tyr Met His Trp Val Lys Gln Ser 
    50                  55                  60                  


His Gly Lys Ser Leu Glu Trp Ile Gly Arg Val Asn Pro Asn Ser Gly 
65                  70                  75                  80  


Gly Thr Ser Tyr Asn Gln Lys Phe Lys Asp Lys Ala Ile Leu Thr Val 
                85                  90                  95      


Asp Lys Ser Ser Ser Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser 
            100                 105                 110         


Glu Asp Ser Ala Val Tyr Tyr Cys Ala Arg Ser Lys Gly Asn Tyr Phe 
        115                 120                 125             


Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 
    130                 135                 140                 


Ser Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp 
145                 150                 155                 160 


Ile Glu Leu Thr Gln Ser Pro Ser Ser Leu Ala Val Ser Leu Gly Gln 
                165                 170                 175     


Arg Ala Thr Ile Ser Cys Arg Ala Ser Glu Ser Val Asp Ser His Gly 
            180                 185                 190         


Thr Ser Leu Met His Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys 
        195                 200                 205             


Phe Leu Ile Tyr Arg Ala Ser Asn Leu Glu Ser Gly Ile Pro Ala Arg 
    210                 215                 220                 


Phe Ser Gly Ser Gly Ser Arg Thr Asp Phe Thr Leu Thr Ile Asn Pro 
225                 230                 235                 240 


Val Glu Thr Asp Asp Val Ala Ile Tyr Tyr Cys Gln Gln Ser Asn Glu 
                245                 250                 255     


Asp Pro Pro Thr Phe Gly Gly Gly Thr Lys Leu Glu Leu Lys Glu Ser 
            260                 265                 270         


Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly 
        275                 280                 285             


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
    290                 295                 300                 


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln 
305                 310                 315                 320 


Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
                325                 330                 335     


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr 
            340                 345                 350         


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
        355                 360                 365             


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile 
    370                 375                 380                 


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
385                 390                 395                 400 


Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser 
                405                 410                 415     


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
            420                 425                 430         


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
        435                 440                 445             


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val 
    450                 455                 460                 


Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met 
465                 470                 475                 480 


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
                485                 490                 495     


Leu Gly Lys Met Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala 
            500                 505                 510         


Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg 
        515                 520                 525             


Ser Lys Arg Ser Arg Gly Gly His Ser Asp Tyr Met Asn Met Thr Pro 
    530                 535                 540                 


Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro 
545                 550                 555                 560 


Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser Ala 
                565                 570                 575     


Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu 
            580                 585                 590         


Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly 
        595                 600                 605             


Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu 
    610                 615                 620                 


Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
625                 630                 635                 640 


Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly 
                645                 650                 655     


Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu 
            660                 665                 670         


His Met Gln Ala Leu Pro Pro Arg 
        675                 680 


<210>  5
<211>  4255
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide sequence

<400>  5
agttgaagtc ggaagtttac atacacttaa gttggagtca ttaaaactcg tttttcaact       60

actccacaaa tttcttgtta acaaacaata gttttggcaa gtcagttagg acatctactt      120

tgtgcatgac acaagtcatt tttccaacaa ttgtttacag acagattatt tcacttataa      180

ttcactgtat cacaattcca gtgggtcaga agtttacata cactaagttg actgtgcctt      240

taaacagctt ggaaaattcc agaaaatgat gtcatggctt tagaagcttc tgatagacta      300

attgacatca tttgagtcaa ttggaggtgt acctgtggat gtatttcaag gaattctgtg      360

gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca      420

aagcatcgag cggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt      480

tgtgtgaatc gtaactaaca tacgctctcc atcaaaacaa aacgaaacaa aacaaactag      540

caaaataggc tgtccccagt gcaagtgcag gtgccagaac atttctctat cgaaggatct      600

gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt      660

tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg      720

aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa      780

gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga      840

agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc      900

cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg      960

ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga     1020

gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg     1080

tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctacct     1140

gagatcaccg gcgaaggagg cctatcatga agatctatcg attgtacagc tagccgccac     1200

catgctgctg ctggtgacca gcctgctgct gtgtgagctg ccccaccccg cctttctgct     1260

gatccccatg gcccaggtga agctgcagca gagcggccct gatctggtga agcctggcgc     1320

cagcgtgaag atcagctgca aggccagcgg ctacagcttc accggctact acatgcactg     1380

ggtgaaacag agccacggca agagcctgga atggatcggc agagtgaacc ccaatagcgg     1440

cggcaccagc tacaaccaga agttcaagga caaggccatc ctgaccgtgg acaagagcag     1500

cagcaccgcc tacatggaac tgcggagcct gaccagcgag gacagcgccg tgtactactg     1560

cgcccggtcc aagggcaact acttctacgc catggactac tggggccagg gcaccaccgt     1620

gaccgtgtct agcagcggcg gaggaagcgg agggggagga tctggcggag gcggcagcga     1680

tatcgagctg acccagagcc ctagcagcct ggccgtgtca ctgggccaga gagccaccat     1740

cagctgcaga gcctccgaga gcgtggatag ccacggcacc agcctgatgc actggtatca     1800

gcagaagccc ggccagcccc ccaagttcct gatctaccgg gccagcaacc tggaaagcgg     1860

catccccgcc agattttccg gcagcggcag cagaaccgac ttcaccctga ccatcaaccc     1920

cgtggagaca gacgacgtgg ccatctacta ctgccagcag agcaacgagg accctcccac     1980

ctttggcgga ggcaccaagc tggaactgaa ggagagcaag tacggccctc cctgcccccc     2040

ttgccctgcc cccgagttcc tgggcggacc cagcgtgttc ctgttccccc ccaagcccaa     2100

ggacaccctg atgatcagcc ggacccccga ggtgacctgt gtggtggtgg acgtgtccca     2160

ggaggacccc gaggtccagt tcaactggta cgtggacggc gtggaggtgc acaacgccaa     2220

gaccaagccc cgggaggagc agttcaatag cacctaccgg gtggtgtccg tgctgaccgt     2280

gctgcaccag gactggctga acggcaagga atacaagtgt aaggtgtcca acaagggcct     2340

gcccagcagc atcgagaaaa ccatcagcaa ggccaagggc cagcctcggg agccccaggt     2400

gtacaccctg ccccctagcc aagaggagat gaccaagaat caggtgtccc tgacctgcct     2460

ggtgaagggc ttctacccca gcgacatcgc cgtggagtgg gagagcaacg gccagcccga     2520

gaacaactac aagaccaccc cccctgtgct ggacagcgac ggcagcttct tcctgtacag     2580

caggctgacc gtggacaaga gccggtggca ggagggcaac gtctttagct gctccgtgat     2640

gcacgaggcc ctgcacaacc actacaccca gaagagcctg tccctgagcc tgggcaagat     2700

gttctgggtg ctggtcgtgg tgggtggcgt gctggcctgc tacagcctgc tggtgacagt     2760

ggccttcatc atcttttggg tgaggagcaa gcggagcaga ggcggccaca gcgactacat     2820

gaacatgacc ccccggaggc ctggccccac ccggaagcac taccagccct acgcccctcc     2880

cagggacttc gccgcctacc ggagccgggt gaagttcagc cggagcgccg acgcccctgc     2940

ctaccagcag ggccagaacc agctgtacaa cgagctgaac ctgggccgga gggaggagta     3000

cgacgtgctg gacaagcgga gaggccggga ccctgagatg ggcggcaagc cccggagaaa     3060

gaaccctcag gagggcctgt ataacgaact gcagaaagac aagatggccg aggcctacag     3120

cgagatcggc atgaagggcg agcggcggag gggcaagggc cacgacggcc tgtaccaggg     3180

cctgagcacc gccaccaagg atacctacga cgccctgcac atgcaggccc tgccccccag     3240

atgactacga cccgggtgat cagcgggatc tgctgtgcct tctagttgcc agccatctgt     3300

tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc     3360

ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg     3420

tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga     3480

tgcggtgggc tctatgggta cccaggtgct gaagaattga cccggttcct cctgggccag     3540

aaagaagcag gcacatcccc ttctctgtga cacaccctgt ccacgcccct ggttcttagt     3600

tccagcccca ctcataggac actcatagct caggagggct ccgccttcaa tcccacccgc     3660

taaagtactt ggagcggtct ctccctccct catcagccca ccaaaccaaa cctagcctcc     3720

aagagtggga agaaattaaa gcaagatagg ctattaagtg cagagggaga gaaaatgcct     3780

ccaacatgtg aggaagtaat gagagaaatc atagaattat cgggccgctg cattctagtt     3840

gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcccatca caaagctctg     3900

acctcaatcc tatagaaagg aggaatgagc caaaattcac ccaacttatt gtgggaagct     3960

tgtggaaggc tactcgaaat gtttgaccca agttaaacaa tttaaaggca atgctaccaa     4020

atactaattg agtgtatgtt aacttctgac ccactgggaa tgtgatgaaa gaaataaaag     4080

ctgaaatgaa tcattctctc tactattatt ctgatatttc acattcttaa aataaagtgg     4140

tgatcctaac tgaccttaag acagggaatc tttactcgga ttaaatgtca ggaattgtga     4200

aaaagtgagt ttaaatgtat ttggctaagg tgtatgtaaa cttccgactt caact          4255


<210>  6
<211>  422
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein

<400>  6

Met Asp Trp Thr Trp Ile Leu Phe Leu Val Ala Ala Ala Thr Arg Val 
1               5                   10                  15      


His Ser Asn Trp Val Asn Val Ile Ser Asp Leu Lys Lys Ile Glu Asp 
            20                  25                  30          


Leu Ile Gln Ser Met His Ile Asp Ala Thr Leu Tyr Thr Glu Ser Asp 
        35                  40                  45              


Val His Pro Ser Cys Lys Val Thr Ala Met Lys Cys Phe Leu Leu Glu 
    50                  55                  60                  


Leu Gln Val Ile Ser Leu Glu Ser Gly Asp Ala Ser Ile His Asp Thr 
65                  70                  75                  80  


Val Glu Asn Leu Ile Ile Leu Ala Asn Asn Ser Leu Ser Ser Asn Gly 
                85                  90                  95      


Asn Val Thr Glu Ser Gly Cys Lys Glu Cys Glu Glu Leu Glu Glu Lys 
            100                 105                 110         


Asn Ile Lys Glu Phe Leu Gln Ser Phe Val His Ile Val Gln Met Phe 
        115                 120                 125             


Ile Asn Thr Ser Ser Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Ser Leu Gln Ile Thr 
145                 150                 155                 160 


Cys Pro Pro Pro Met Ser Val Glu His Ala Asp Ile Trp Val Lys Ser 
                165                 170                 175     


Tyr Ser Leu Tyr Ser Arg Glu Arg Tyr Ile Cys Asn Ser Gly Phe Lys 
            180                 185                 190         


Arg Lys Ala Gly Thr Ser Ser Leu Thr Glu Cys Val Leu Asn Lys Ala 
        195                 200                 205             


Thr Asn Val Ala His Trp Thr Thr Pro Ser Leu Lys Cys Ile Arg Asp 
    210                 215                 220                 


Pro Ala Leu Val His Gln Arg Pro Ala Pro Pro Ser Thr Val Thr Thr 
225                 230                 235                 240 


Ala Gly Val Thr Pro Gln Pro Glu Ser Leu Ser Pro Ser Gly Lys Glu 
                245                 250                 255     


Pro Ala Ala Ser Ser Pro Ser Ser Asn Asn Thr Ala Ala Thr Thr Ala 
            260                 265                 270         


Ala Ile Val Pro Gly Ser Gln Leu Met Pro Ser Lys Ser Pro Ser Thr 
        275                 280                 285             


Gly Thr Thr Glu Ile Ser Ser His Glu Ser Ser His Gly Thr Pro Ser 
    290                 295                 300                 


Gln Thr Thr Ala Lys Asn Trp Glu Leu Thr Ala Ser Ala Ser His Gln 
305                 310                 315                 320 


Pro Pro Gly Val Tyr Pro Gln Gly His Ser Asp Thr Thr Val Ala Ile 
                325                 330                 335     


Ser Thr Ser Thr Val Leu Leu Cys Gly Leu Ser Ala Val Ser Leu Leu 
            340                 345                 350         


Ala Cys Tyr Leu Lys Ser Arg Gln Thr Pro Pro Leu Ala Ser Val Glu 
        355                 360                 365             


Met Glu Ala Met Glu Ala Leu Pro Val Thr Trp Gly Thr Ser Ser Arg 
    370                 375                 380                 


Asp Glu Asp Leu Glu Asn Cys Ser His His Leu Ser Arg Met Asp Tyr 
385                 390                 395                 400 


Lys Asp Asp Asp Asp Lys Asp Tyr Lys Asp Asp Asp Asp Lys Asp Tyr 
                405                 410                 415     


Lys Asp Asp Asp Asp Lys 
            420         


<210>  7
<211>  3499
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic polynucleotide sequence

<400>  7
agttgaagtc ggaagtttac atacacttaa gttggagtca ttaaaactcg tttttcaact       60

actccacaaa tttcttgtta acaaacaata gttttggcaa gtcagttagg acatctactt      120

tgtgcatgac acaagtcatt tttccaacaa ttgtttacag acagattatt tcacttataa      180

ttcactgtat cacaattcca gtgggtcaga agtttacata cactaagttg actgtgcctt      240

taaacagctt ggaaaattcc agaaaatgat gtcatggctt tagaagcttc tgatagacta      300

attgacatca tttgagtcaa ttggaggtgt acctgtggat gtatttcaag gaattctgtg      360

gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca      420

aagcatcgag cggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt      480

tgtgtgaatc gtaactaaca tacgctctcc atcaaaacaa aacgaaacaa aacaaactag      540

caaaataggc tgtccccagt gcaagtgcag gtgccagaac atttctctat cgaaggatct      600

gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt      660

tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg      720

aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa      780

gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga      840

agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc      900

cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg      960

ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga     1020

gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg     1080

tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctacct     1140

gagatcaccg gcgaaggagg cctatcatga agatctatcg attgtacagc tagccgccac     1200

catggattgg acctggattc tgtttctggt ggccgctgcc acaagagtgc acagcaactg     1260

ggtgaatgtg atcagcgacc tgaagaagat cgaggatctg atccagagca tgcacattga     1320

tgccaccctg tacacagaat ctgatgtgca ccctagctgt aaagtgaccg ccatgaagtg     1380

ttttctgctg gagctgcagg tgatttctct ggaaagcgga gatgcctcta tccacgacac     1440

agtggagaat ctgatcatcc tggccaacaa tagcctgagc agcaatggca atgtgacaga     1500

gtctggctgt aaggagtgtg aggagctgga ggagaagaac atcaaggagt ttctgcagag     1560

ctttgtgcac atcgtgcaga tgttcatcaa tacaagctct ggcggaggat ctggaggagg     1620

cggatctgga ggaggaggca gtggaggcgg aggatctggc ggaggatctc tgcagattac     1680

atgccctcct ccaatgtctg tggagcacgc cgatatttgg gtgaagtcct acagcctgta     1740

cagcagagag agatacatct gcaacagcgg ctttaagaga aaggccggca cctcttctct     1800

gacagagtgc gtgctgaata aggccacaaa tgtggcccac tggacaacac ctagcctgaa     1860

gtgcattaga gatcctgccc tggtccacca gaggcctgcc cctccatcta cagtgacaac     1920

agccggagtg acacctcagc ctgaatctct gagcccttct ggaaaagaac ctgccgccag     1980

ctctcctagc tctaataata ccgccgccac aacagccgcc attgtgcctg gatctcagct     2040

gatgcctagc aagtctccta gcacaggcac aacagagatc agcagccacg aatcttctca     2100

cggaacacct tctcagacca ccgccaagaa ttgggagctg acagcctctg cctctcacca     2160

gcctccagga gtgtatcctc agggccactc tgatacaaca gtggccatca gcacatctac     2220

agtgctgctg tgtggactgt ctgccgtgtc tctgctggcc tgttacctga agtctagaca     2280

gacacctcct ctggcctctg tggagatgga ggccatggaa gccctgcctg tgacatgggg     2340

aacaagcagc agagatgagg acctggagaa ttgttctcac cacctgtcgc gaatggacta     2400

caaggacgat gacgacaagg attataaaga tgatgatgat aaagattata aagacgacga     2460

tgataagtcg cgatgatgat gactcgagac tagtcccggg tgatcagcgg gatctgctgt     2520

gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga     2580

aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag     2640

taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga     2700

agacaatagc aggcatgctg gggatgcggt gggctctatg ggtacccagg tgctgaagaa     2760

ttgacccggt tcctcctggg ccagaaagaa gcaggcacat ccccttctct gtgacacacc     2820

ctgtccacgc ccctggttct tagttccagc cccactcata ggacactcat agctcaggag     2880

ggctccgcct tcaatcccac ccgctaaagt acttggagcg gtctctccct ccctcatcag     2940

cccaccaaac caaacctagc ctccaagagt gggaagaaat taaagcaaga taggctatta     3000

agtgcagagg gagagaaaat gcctccaaca tgtgaggaag taatgagaga aatcatagaa     3060

ttatcgggcc gctgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg     3120

tctggatccc atcacaaagc tctgacctca atcctataga aaggaggaat gagccaaaat     3180

tcacccaact tattgtggga agcttgtgga aggctactcg aaatgtttga cccaagttaa     3240

acaatttaaa ggcaatgcta ccaaatacta attgagtgta tgttaacttc tgacccactg     3300

ggaatgtgat gaaagaaata aaagctgaaa tgaatcattc tctctactat tattctgata     3360

tttcacattc ttaaaataaa gtggtgatcc taactgacct taagacaggg aatctttact     3420

cggattaaat gtcaggaatt gtgaaaaagt gagtttaaat gtatttggct aaggtgtatg     3480

taaacttccg acttcaact                                                  3499


<210>  8
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  8
cagcgacggc agcttctt                                                     18


<210>  9
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  9
tgcatcacgg agctaaa                                                      17


<210>  10
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  10
agagccggtg gcagg                                                        15


<210>  11
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  11
atgggaaaat caaaagaaat c                                                 21


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  12
ctagtatttg gtagcattgc                                                   20


<210>  13
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  13
tctccagaac atcatccctg ccac                                              24


<210>  14
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  14
tgggccatga ggtccaccac cctg                                              24


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  15
gagggcaacg tctttagctg                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  16
gatgatgaag gccactgtca                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  17
agatgttctg ggtgctggtc                                                   20


