                                SEQUENCE LISTING

<110> GnuBO, Inc.

<120> Systems and Methods for Sequencing in
  Emulsion Based Microfluidics
  

<130> 94868-910118

<140> WO PCT/US14/40082   
<141> 2014-05-29  

<150> US 61/828,582       
<151> 2013-05-29  

<160> 12

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 11
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic positive primer "G"

<400> 1
cagtaaaaat a                                                      11

<210> 2
<211> 12
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer "C"

<400> 2
ctacagagaa at                                                     12

<210> 3
<211> 64
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic wild-type BRAF exon 15 amplicon
      reference subsequence "D"

<400> 3
acagtaaaaa taggtgattt tggtctagct acaatgaaat ctcgatggag tgggtcccat 60
cagt                                                              64

<210> 4
<211> 64
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic final consensus target sequence "I"

<400> 4
acagtaaaaa taggtgattt tggtctagct acagagaaat ctcgatggag tgggtcccat 60
cagt                                                              64

<210> 5
<211> 38
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic wild-type reference sequence 1000,
      reference sequence 1400, stage 6 final assembly

<400> 5
tagctatgac gtagggtact tagatgctga ccgttagc                         38

<210> 6
<211> 38
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic target molecule

<400> 6
aatgggtagg gggggtataa ttgagagaga tgaggtgt                         38

<210> 7
<211> 38
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic stage 2 initial scaffold, assembly scaffold,
      initial target scaffold sequence

<220> 
<221> modified_base  
<222> (1)...(38)
<223> n = g, a, c or t

<400> 7
antnggtngn gngggnanan ttgngngnga tnangngt                         38

<210> 8
<211> 38
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic stage 7 reference sequence (optional)

<220> 
<221> modified_base  
<222> (23)...(23)
<223> n = g, a, c or t

<400> 8
aatgggtagg gtgggtataa ttnagagaga tgaggtgt                         38

<210> 9
<211> 46
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic reference sequence 1700, target nucleic acid,
      wild-type or "reference" allele

<220> 
<221> modified_base  
<222> (36)...(36)
<223> n = g, a, c or t

<400> 9
aaaaacaacc gagctccgac ggatttttgt tcgcantttt gtatat                46

<210> 10
<211> 10
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer

<400> 10
attttaactt                                                        10

<210> 11
<211> 10
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer partition library supplemental
      dinucleotide repeat primer

<400> 11
atatatatat                                                        10

<210> 12
<211> 10
<212> DNA
<213> Artificial Sequence

<220> 
<223> synthetic primer partition library supplemental
      homopolymer primer

<400> 12
tttttttttt                                                        10


