                         SEQUENCE LISTING

<110>  BEN GURION UNIVERSITY OF THE NEGEV RESEARCH AND DEVELOPMENT
        AUTHORITY
       The Regents of the University of Colorado, a body corporate
 
<120>  IL-1beta-IL-1Ra CHIMERA AND METHODS OF USING THE SAME

<130>  BGU-P-036-PCT

<150>  61/831,318
<151>  2013-06-05

<160>  24    

<170>  PatentIn version 3.5

<210>  1
<211>  120
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Ala Glu Val Pro Glu Leu Ala Ser Glu Met Met Ala Tyr Tyr Ser 
1               5                   10                  15      


Gly Asn Glu Asp Asp Leu Phe Phe Glu Ala Asp Gly Pro Lys Gln Met 
            20                  25                  30          


Lys Cys Ser Phe Gln Asp Leu Asp Leu Cys Pro Leu Asp Gly Gly Ile 
        35                  40                  45              


Gln Leu Arg Ile Ser Asp His His Tyr Ser Lys Gly Phe Arg Gln Ala 
    50                  55                  60                  


Ala Ser Val Val Val Ala Met Asp Lys Leu Arg Lys Met Leu Val Pro 
65                  70                  75                  80  


Cys Pro Gln Thr Phe Gln Glu Asn Asp Leu Ser Thr Phe Phe Pro Phe 
                85                  90                  95      


Ile Phe Glu Glu Glu Pro Ile Phe Phe Asp Thr Trp Asp Asn Glu Ala 
            100                 105                 110         


Tyr Val His Asp Ala Pro Val Arg 
        115                 120 


<210>  2
<211>  152
<212>  PRT
<213>  Homo sapiens

<400>  2

Arg Pro Ser Gly Arg Lys Ser Ser Lys Met Gln Ala Phe Arg Ile Trp 
1               5                   10                  15      


Asp Val Asn Gln Lys Thr Phe Tyr Leu Arg Asn Asn Gln Leu Val Ala 
            20                  25                  30          


Gly Tyr Leu Gln Gly Pro Asn Val Asn Leu Glu Glu Lys Ile Asp Val 
        35                  40                  45              


Val Pro Ile Glu Pro His Ala Leu Phe Leu Gly Ile His Gly Gly Lys 
    50                  55                  60                  


Met Cys Leu Ser Cys Val Lys Ser Gly Asp Glu Thr Arg Leu Gln Leu 
65                  70                  75                  80  


Glu Ala Val Asn Ile Thr Asp Leu Ser Glu Asn Arg Lys Gln Asp Lys 
                85                  90                  95      


Arg Phe Ala Phe Ile Arg Ser Asp Ser Gly Pro Thr Thr Ser Phe Glu 
            100                 105                 110         


Ser Ala Ala Cys Pro Gly Trp Phe Leu Cys Thr Ala Met Glu Ala Asp 
        115                 120                 125             


Gln Pro Val Ser Leu Thr Asn Met Pro Asp Glu Gly Val Met Val Thr 
    130                 135                 140                 


Lys Phe Tyr Phe Gln Glu Asp Glu 
145                 150         


<210>  3
<211>  274
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic

<400>  3

Met Ala Glu Val Pro Glu Leu Ala Ser Glu Met Met Ala Tyr Tyr Ser 
1               5                   10                  15      


Gly Asn Glu Asp Asp Leu Phe Phe Glu Ala Asp Gly Pro Lys Gln Met 
            20                  25                  30          


Lys Cys Ser Phe Gln Asp Leu Asp Leu Cys Pro Leu Asp Gly Gly Ile 
        35                  40                  45              


Gln Leu Arg Ile Ser Asp His His Tyr Ser Lys Gly Phe Arg Gln Ala 
    50                  55                  60                  


Ala Ser Val Val Val Ala Met Asp Lys Leu Arg Lys Met Leu Val Pro 
65                  70                  75                  80  


Cys Pro Gln Thr Phe Gln Glu Asn Asp Leu Ser Thr Phe Phe Pro Phe 
                85                  90                  95      


Ile Phe Glu Glu Glu Pro Ile Phe Phe Asp Thr Trp Asp Asn Glu Ala 
            100                 105                 110         


Tyr Val His Asp Ala Pro Val Arg Lys Leu Arg Pro Ser Gly Arg Lys 
        115                 120                 125             


Ser Ser Lys Met Gln Ala Phe Arg Ile Trp Asp Val Asn Gln Lys Thr 
    130                 135                 140                 


Phe Tyr Leu Arg Asn Asn Gln Leu Val Ala Gly Tyr Leu Gln Gly Pro 
145                 150                 155                 160 


Asn Val Asn Leu Glu Glu Lys Ile Asp Val Val Pro Ile Glu Pro His 
                165                 170                 175     


Ala Leu Phe Leu Gly Ile His Gly Gly Lys Met Cys Leu Ser Cys Val 
            180                 185                 190         


Lys Ser Gly Asp Glu Thr Arg Leu Gln Leu Glu Ala Val Asn Ile Thr 
        195                 200                 205             


Asp Leu Ser Glu Asn Arg Lys Gln Asp Lys Arg Phe Ala Phe Ile Arg 
    210                 215                 220                 


Ser Asp Ser Gly Pro Thr Thr Ser Phe Glu Ser Ala Ala Cys Pro Gly 
225                 230                 235                 240 


Trp Phe Leu Cys Thr Ala Met Glu Ala Asp Gln Pro Val Ser Leu Thr 
                245                 250                 255     


Asn Met Pro Asp Glu Gly Val Met Val Thr Lys Phe Tyr Phe Gln Glu 
            260                 265                 270         


Asp Glu 
        


<210>  4
<211>  360
<212>  PRT
<213>  Homo sapiens

<400>  4

Ala Thr Gly Gly Cys Ala Gly Ala Ala Gly Thr Ala Cys Cys Thr Gly 
1               5                   10                  15      


Ala Gly Cys Thr Cys Gly Cys Cys Ala Gly Thr Gly Ala Ala Ala Thr 
            20                  25                  30          


Gly Ala Thr Gly Gly Cys Thr Thr Ala Thr Thr Ala Cys Ala Gly Thr 
        35                  40                  45              


Gly Gly Cys Ala Ala Thr Gly Ala Gly Gly Ala Thr Gly Ala Cys Thr 
    50                  55                  60                  


Thr Gly Thr Thr Cys Thr Thr Thr Gly Ala Ala Gly Cys Thr Gly Ala 
65                  70                  75                  80  


Thr Gly Gly Cys Cys Cys Thr Ala Ala Ala Cys Ala Gly Ala Thr Gly 
                85                  90                  95      


Ala Ala Gly Thr Gly Cys Thr Cys Cys Thr Thr Cys Cys Ala Gly Gly 
            100                 105                 110         


Ala Cys Cys Thr Gly Gly Ala Cys Cys Thr Cys Thr Gly Cys Cys Cys 
        115                 120                 125             


Thr Cys Thr Gly Gly Ala Thr Gly Gly Cys Gly Gly Cys Ala Thr Cys 
    130                 135                 140                 


Cys Ala Gly Cys Thr Ala Cys Gly Ala Ala Thr Cys Thr Cys Cys Gly 
145                 150                 155                 160 


Ala Cys Cys Ala Cys Cys Ala Cys Thr Ala Cys Ala Gly Cys Ala Ala 
                165                 170                 175     


Gly Gly Gly Cys Thr Thr Cys Ala Gly Gly Cys Ala Gly Gly Cys Cys 
            180                 185                 190         


Gly Cys Gly Thr Cys Ala Gly Thr Thr Gly Thr Thr Gly Thr Gly Gly 
        195                 200                 205             


Cys Cys Ala Thr Gly Gly Ala Cys Ala Ala Gly Cys Thr Gly Ala Gly 
    210                 215                 220                 


Gly Ala Ala Gly Ala Thr Gly Cys Thr Gly Gly Thr Thr Cys Cys Cys 
225                 230                 235                 240 


Thr Gly Cys Cys Cys Ala Cys Ala Gly Ala Cys Cys Thr Thr Cys Cys 
                245                 250                 255     


Ala Gly Gly Ala Gly Ala Ala Thr Gly Ala Cys Cys Thr Gly Ala Gly 
            260                 265                 270         


Cys Ala Cys Cys Thr Thr Cys Thr Thr Thr Cys Cys Cys Thr Thr Cys 
        275                 280                 285             


Ala Thr Cys Thr Thr Thr Gly Ala Ala Gly Ala Ala Gly Ala Ala Cys 
    290                 295                 300                 


Cys Thr Ala Thr Cys Thr Thr Cys Thr Thr Cys Gly Ala Cys Ala Cys 
305                 310                 315                 320 


Ala Thr Gly Gly Gly Ala Thr Ala Ala Cys Gly Ala Gly Gly Cys Thr 
                325                 330                 335     


Thr Ala Thr Gly Thr Gly Cys Ala Cys Gly Ala Thr Gly Cys Ala Cys 
            340                 345                 350         


Cys Thr Gly Thr Ala Cys Gly Ala 
        355                 360 


<210>  5
<211>  459
<212>  DNA
<213>  Homo sapiens

<400>  5
cgaccctctg ggagaaaatc cagcaagatg caagccttca gaatctggga tgttaaccag       60

aagaccttct atctgaggaa caaccaacta gttgctggat acttgcaagg accaaatgtc      120

aatttagaag aaaagataga tgtggtaccc attgagcctc atgctctgtt cttgggaatc      180

catggaggga agatgtgcct gtcctgtgtc aagtctggtg atgagaccag actccagctg      240

gaggcagtta acatcactga cctgagcgag aacagaaagc aggacaagcg cttcgccttc      300

atccgctcag acagtggccc caccaccagt tttgagtctg ccgcctgccc cggttggttc      360

ctctgcacag cgatggaagc tgaccagccc gtcagcctca ccaatatgcc tgacgaaggc      420

gtcatggtca ccaaattcta cttccaggag gacgagtag                             459


<210>  6
<211>  825
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic

<400>  6
atggcagaag tacctgagct cgccagtgaa atgatggctt attacagtgg caatgaggat       60

gacttgttct ttgaagctga tggccctaaa cagatgaagt gctccttcca ggacctggac      120

ctctgccctc tggatggcgg catccagcta cgaatctccg accaccacta cagcaagggc      180

ttcaggcagg ccgcgtcagt tgttgtggcc atggacaagc tgaggaagat gctggttccc      240

tgcccacaga ccttccagga gaatgacctg agcaccttct ttcccttcat ctttgaagaa      300

gaacctatct tcttcgacac atgggataac gaggcttatg tgcacgatgc acctgtacga      360

aagcttcgac cctctgggag aaaatccagc aagatgcaag ccttcagaat ctgggatgtt      420

aaccagaaga ccttctatct gaggaacaac caactagttg ctggatactt gcaaggacca      480

aatgtcaatt tagaagaaaa gatagatgtg gtacccattg agcctcatgc tctgttcttg      540

ggaatccatg gagggaagat gtgcctgtcc tgtgtcaagt ctggtgatga gaccagactc      600

cagctggagg cagttaacat cactgacctg agcgagaaca gaaagcagga caagcgcttc      660

gccttcatcc gctcagacag tggccccacc accagttttg agtctgccgc ctgccccggt      720

tggttcctct gcacagcgat ggaagctgac cagcccgtca gcctcaccaa tatgcctgac      780

gaaggcgtca tggtcaccaa attctacttc caggaggacg agtag                      825


<210>  7
<211>  153
<212>  PRT
<213>  Homo sapiens

<400>  7

Ala Pro Val Arg Ser Leu Asn Cys Thr Leu Arg Asp Ser Gln Gln Lys 
1               5                   10                  15      


Ser Leu Val Met Ser Gly Pro Tyr Glu Leu Lys Ala Leu His Leu Gln 
            20                  25                  30          


Gly Gln Asp Met Glu Gln Gln Val Val Phe Ser Met Ser Phe Val Gln 
        35                  40                  45              


Gly Glu Glu Ser Asn Asp Lys Ile Pro Val Ala Leu Gly Leu Lys Glu 
    50                  55                  60                  


Lys Asn Leu Tyr Leu Ser Cys Val Leu Lys Asp Asp Lys Pro Thr Leu 
65                  70                  75                  80  


Gln Leu Glu Ser Val Asp Pro Lys Asn Tyr Pro Lys Lys Lys Met Glu 
                85                  90                  95      


Lys Arg Phe Val Phe Asn Lys Ile Glu Ile Asn Asn Lys Leu Glu Phe 
            100                 105                 110         


Glu Ser Ala Gln Phe Pro Asn Trp Tyr Ile Ser Thr Ser Gln Ala Glu 
        115                 120                 125             


Asn Met Pro Val Phe Leu Gly Gly Thr Lys Gly Gly Gln Asp Ile Thr 
    130                 135                 140                 


Asp Phe Thr Met Gln Phe Val Ser Ser 
145                 150             


<210>  8
<211>  269
<212>  PRT
<213>  Homo sapiens

<400>  8

Met Ala Glu Val Pro Glu Leu Ala Ser Glu Met Met Ala Tyr Tyr Ser 
1               5                   10                  15      


Gly Asn Glu Asp Asp Leu Phe Phe Glu Ala Asp Gly Pro Lys Gln Met 
            20                  25                  30          


Lys Cys Ser Phe Gln Asp Leu Asp Leu Cys Pro Leu Asp Gly Gly Ile 
        35                  40                  45              


Gln Leu Arg Ile Ser Asp His His Tyr Ser Lys Gly Phe Arg Gln Ala 
    50                  55                  60                  


Ala Ser Val Val Val Ala Met Asp Lys Leu Arg Lys Met Leu Val Pro 
65                  70                  75                  80  


Cys Pro Gln Thr Phe Gln Glu Asn Asp Leu Ser Thr Phe Phe Pro Phe 
                85                  90                  95      


Ile Phe Glu Glu Glu Pro Ile Phe Phe Asp Thr Trp Asp Asn Glu Ala 
            100                 105                 110         


Tyr Val His Asp Ala Pro Val Arg Ser Leu Asn Cys Thr Leu Arg Asp 
        115                 120                 125             


Ser Gln Gln Lys Ser Leu Val Met Ser Gly Pro Tyr Glu Leu Lys Ala 
    130                 135                 140                 


Leu His Leu Gln Gly Gln Asp Met Glu Gln Gln Val Val Phe Ser Met 
145                 150                 155                 160 


Ser Phe Val Gln Gly Glu Glu Ser Asn Asp Lys Ile Pro Val Ala Leu 
                165                 170                 175     


Gly Leu Lys Glu Lys Asn Leu Tyr Leu Ser Cys Val Leu Lys Asp Asp 
            180                 185                 190         


Lys Pro Thr Leu Gln Leu Glu Ser Val Asp Pro Lys Asn Tyr Pro Lys 
        195                 200                 205             


Lys Lys Met Glu Lys Arg Phe Val Phe Asn Lys Ile Glu Ile Asn Asn 
    210                 215                 220                 


Lys Leu Glu Phe Glu Ser Ala Gln Phe Pro Asn Trp Tyr Ile Ser Thr 
225                 230                 235                 240 


Ser Gln Ala Glu Asn Met Pro Val Phe Leu Gly Gly Thr Lys Gly Gly 
                245                 250                 255     


Gln Asp Ile Thr Asp Phe Thr Met Gln Phe Val Ser Ser 
            260                 265                 


<210>  9
<211>  50
<212>  PRT
<213>  Homo sapiens

<400>  9

Met Asp Lys Leu Arg Lys Met Leu Val Pro Cys Pro Gln Thr Phe Gln 
1               5                   10                  15      


Glu Asn Asp Leu Ser Thr Phe Phe Pro Phe Ile Phe Glu Glu Glu Pro 
            20                  25                  30          


Ile Phe Phe Asp Thr Trp Asp Asn Glu Ala Tyr Val His Asp Ala Pro 
        35                  40                  45              


Val Arg 
    50  


<210>  10
<211>  204
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic

<400>  10

Met Asp Lys Leu Arg Lys Met Leu Val Pro Cys Pro Gln Thr Phe Gln 
1               5                   10                  15      


Glu Asn Asp Leu Ser Thr Phe Phe Pro Phe Ile Phe Glu Glu Glu Pro 
            20                  25                  30          


Ile Phe Phe Asp Thr Trp Asp Asn Glu Ala Tyr Val His Asp Ala Pro 
        35                  40                  45              


Val Arg Lys Leu Arg Pro Ser Gly Arg Lys Ser Ser Lys Met Gln Ala 
    50                  55                  60                  


Phe Arg Ile Trp Asp Val Asn Gln Lys Thr Phe Tyr Leu Arg Asn Asn 
65                  70                  75                  80  


Gln Leu Val Ala Gly Tyr Leu Gln Gly Pro Asn Val Asn Leu Glu Glu 
                85                  90                  95      


Lys Ile Asp Val Val Pro Ile Glu Pro His Ala Leu Phe Leu Gly Ile 
            100                 105                 110         


His Gly Gly Lys Met Cys Leu Ser Cys Val Lys Ser Gly Asp Glu Thr 
        115                 120                 125             


Arg Leu Gln Leu Glu Ala Val Asn Ile Thr Asp Leu Ser Glu Asn Arg 
    130                 135                 140                 


Lys Gln Asp Lys Arg Phe Ala Phe Ile Arg Ser Asp Ser Gly Pro Thr 
145                 150                 155                 160 


Thr Ser Phe Glu Ser Ala Ala Cys Pro Gly Trp Phe Leu Cys Thr Ala 
                165                 170                 175     


Met Glu Ala Asp Gln Pro Val Ser Leu Thr Asn Met Pro Asp Glu Gly 
            180                 185                 190         


Val Met Val Thr Lys Phe Tyr Phe Gln Glu Asp Glu 
        195                 200                 


<210>  11
<211>  150
<212>  DNA
<213>  Homo sapiens

<400>  11
atggacaagc tgaggaagat gctggttccc tgcccacaga ccttccagga gaatgacctg       60

agcaccttct ttcccttcat ctttgaagaa gaacctatct tcttcgacac atgggataac      120

gaggcttatg tgcacgatgc acctgtacga                                       150


<210>  12
<211>  615
<212>  DNA
<213>  Artifical

<400>  12
atggacaagc tgaggaagat gctggttccc tgcccacaga ccttccagga gaatgacctg       60

agcaccttct ttcccttcat ctttgaagaa gaacctatct tcttcgacac atgggataac      120

gaggcttatg tgcacgatgc acctgtacga aagcttcgac cctctgggag aaaatccagc      180

aagatgcaag ccttcagaat ctgggatgtt aaccagaaga ccttctatct gaggaacaac      240

caactagttg ctggatactt gcaaggacca aatgtcaatt tagaagaaaa gatagatgtg      300

gtacccattg agcctcatgc tctgttcttg ggaatccatg gagggaagat gtgcctgtcc      360

tgtgtcaagt ctggtgatga gaccagactc cagctggagg cagttaacat cactgacctg      420

agcgagaaca gaaagcagga caagcgcttc gccttcatcc gctcagacag tggccccacc      480

accagttttg agtctgccgc ctgccccggt tggttcctct gcacagcgat ggaagctgac      540

cagcccgtca gcctcaccaa tatgcctgac gaaggcgtca tggtcaccaa attctacttc      600

caggaggacg agtag                                                       615


<210>  13
<211>  19
<212>  PRT
<213>  Homo sapiens

<400>  13

Pro Ile Phe Phe Asp Thr Trp Asp Asn Glu Ala Tyr Val His Asp Ala 
1               5                   10                  15      


Pro Val Arg 
            


<210>  14
<211>  36
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic

<400>  14
cacggatccg ccaccatggc agaagtacct gagctc                                 36


<210>  15
<211>  35
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic

<400>  15
cacaagcttt cgtacaggtg catcgtgcac ataag                                  35


<210>  16
<211>  32
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic

<400>  16
cacaagcttc gaccctctgg gagaaaatcc ag                                     32


<210>  17
<211>  37
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic

<400>  17
tcacagtgcg gccgcctact cgtcctcctg gaagtag                                37


<210>  18
<211>  40
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic

<400>  18
caggatccgc caccatgcga ccctctggga gaaaatccag                             40


<210>  19
<211>  22
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic

<400>  19
cgaccctctg ggagaaaatc ca                                                22


<210>  20
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic

<400>  20
ttcttcttca aagatgaagg gaaagaaggt                                        30


<210>  21
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic

<400>  21
atggacaagc tgaggaagat gctg                                              24


<210>  22
<211>  25
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic

<400>  22
gcagatcgtc tctgaatgga acagg                                             25


<210>  23
<211>  36
<212>  DNA
<213>  Artificial

<220>
<223>  Synthetic

<400>  23
cacggatcca tggacaagaa gctgaggaag atgctg                                 36


<210>  24
<211>  4
<212>  PRT
<213>  Homo sapiens

<400>  24

Tyr Val His Asp 
1               


