                               SEQUENCE LISTING

<110> UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR 
      COMMERCIALIZATION
 
<120> SOLUBLE MIC NEUTRALIZING MONOCLONAL ANTIBODY FOR TREATING CANCER

<130> 034186-081580-PCT

<140>
<141>

<150> 61/893,734
<151> 2013-10-21

<150> 61/843,182
<151> 2013-07-05

<160> 20    

<170> PatentIn version 3.5

<210> 1
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 1
gcccacatta ataattgg                                                     18


<210> 2
<211> 9
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 2
gatgcaacc                                                                9


<210> 3
<211> 27
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 3
caacattatt ggagtactcc gtggacg                                           27


<210> 4
<211> 27
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 4
ggctactcaa tcaccagtga ttatgcc                                           27


<210> 5
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 5
ataagctaca gtggtagcac t                                                 21


<210> 6
<211> 27
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 6
gcaagggggg ggacttactt tgactac                                           27


<210> 7
<211> 116
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 7
Asp Ile Val Leu Thr Gln Thr Thr Ser Tyr Leu Ser Val Ser Leu Gly 
1               5                   10                  15      


Gly Arg Val Thr Ile Ala Cys Lys Ala Ser Ala His Ile Asn Asn Trp 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Asn Ala Pro Arg Leu Leu Ile 
        35                  40                  45              


Ser Asp Ala Thr Ser Leu Glu Thr Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Lys Asp Tyr Thr Leu Ser Ile Thr Ser Leu Gln Thr 
65                  70                  75                  80  


Glu Asp Val Ala Thr Tyr Tyr Cys Gln His Tyr Trp Ser Thr Pro Trp 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Ala Asp Ala Ala 
            100                 105                 110         


Pro Thr Val Ser 
        115     


<210> 8
<211> 127
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 8
Leu Pro Glu Val Gln Leu Glu Glu Ser Gly Pro Gly Leu Val Lys Pro 
1               5                   10                  15      


Ser Gln Ser Leu Ser Leu Thr Cys Thr Val Thr Gly Tyr Ser Ile Thr 
            20                  25                  30          


Ser Asp Tyr Ala Trp Asn Trp Ile Arg Gln Phe Pro Gly Asn Lys Leu 
        35                  40                  45              


Glu Trp Met Gly Tyr Ile Ser Tyr Ser Gly Ser Thr Asn Tyr Asn Pro 
    50                  55                  60                  


Ser Leu Lys Ser Arg Ile Ser Ile Thr Arg Asp Thr Ser Lys Asn Gln 
65                  70                  75                  80  


Phe Phe Leu Gln Leu Asn Ser Val Ile Thr Glu Asp Thr Ala Thr Tyr 
                85                  90                  95      


Tyr Cys Ala Arg Gly Gly Thr Tyr Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Thr Leu Thr Val Ser Ser Ala Lys Thr Thr Pro Pro Ser Val Tyr 
        115                 120                 125         


<210> 9
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 9
Ala His Ile Asn Asn Trp 
1               5       


<210> 10
<211> 3
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 10
Asp Ala Thr 
1           


<210> 11
<211> 9
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 11
Gln His Tyr Trp Ser Thr Pro Trp Thr 
1               5                   


<210> 12
<211> 9
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 12
Gly Tyr Ser Ile Thr Ser Asp Tyr Ala 
1               5                   


<210> 13
<211> 7
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 13
Ile Ser Tyr Ser Gly Ser Thr 
1               5           


<210> 14
<211> 9
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 14
Ala Arg Gly Gly Thr Tyr Phe Asp Tyr 
1               5                   


<210> 15
<211> 383
<212> PRT
<213> Homo sapiens

<400> 15
Met Gly Leu Gly Pro Val Phe Leu Leu Leu Ala Gly Ile Phe Pro Phe 
1               5                   10                  15      


Ala Pro Pro Gly Ala Ala Ala Glu Pro His Ser Leu Arg Tyr Asn Leu 
            20                  25                  30          


Thr Val Leu Ser Trp Asp Gly Ser Val Gln Ser Gly Phe Leu Thr Glu 
        35                  40                  45              


Val His Leu Asp Gly Gln Pro Phe Leu Arg Cys Asp Arg Gln Lys Cys 
    50                  55                  60                  


Arg Ala Lys Pro Gln Gly Gln Trp Ala Glu Asp Val Leu Gly Asn Lys 
65                  70                  75                  80  


Thr Trp Asp Arg Glu Thr Arg Asp Leu Thr Gly Asn Gly Lys Asp Leu 
                85                  90                  95      


Arg Met Thr Leu Ala His Ile Lys Asp Gln Lys Glu Gly Leu His Ser 
            100                 105                 110         


Leu Gln Glu Ile Arg Val Cys Glu Ile His Glu Asp Asn Ser Thr Arg 
        115                 120                 125             


Ser Ser Gln His Phe Tyr Tyr Asp Gly Glu Leu Phe Leu Ser Gln Asn 
    130                 135                 140                 


Leu Glu Thr Lys Glu Trp Thr Met Pro Gln Ser Ser Arg Ala Gln Thr 
145                 150                 155                 160 


Leu Ala Met Asn Val Arg Asn Phe Leu Lys Glu Asp Ala Met Lys Thr 
                165                 170                 175     


Lys Thr His Tyr His Ala Met His Ala Asp Cys Leu Gln Glu Leu Arg 
            180                 185                 190         


Arg Tyr Leu Lys Ser Gly Val Val Leu Arg Arg Thr Val Pro Pro Met 
        195                 200                 205             


Val Asn Val Thr Arg Ser Glu Ala Ser Glu Gly Asn Ile Thr Val Thr 
    210                 215                 220                 


Cys Arg Ala Ser Gly Phe Tyr Pro Trp Asn Ile Thr Leu Ser Trp Arg 
225                 230                 235                 240 


Gln Asp Gly Val Ser Leu Ser His Asp Thr Gln Gln Trp Gly Asp Val 
                245                 250                 255     


Leu Pro Asp Gly Asn Gly Thr Tyr Gln Thr Trp Val Ala Thr Arg Ile 
            260                 265                 270         


Cys Gln Gly Glu Glu Gln Arg Phe Thr Cys Tyr Met Glu His Ser Gly 
        275                 280                 285             


Asn His Ser Thr His Pro Val Pro Ser Gly Lys Val Leu Val Leu Gln 
    290                 295                 300                 


Ser His Trp Gln Thr Phe His Val Ser Ala Val Ala Ala Ala Ala Ile 
305                 310                 315                 320 


Phe Val Ile Ile Ile Phe Tyr Val Arg Cys Cys Lys Lys Lys Thr Ser 
                325                 330                 335     


Ala Ala Glu Gly Pro Glu Leu Val Ser Leu Gln Val Leu Asp Gln His 
            340                 345                 350         


Pro Val Gly Thr Ser Asp His Arg Asp Ala Thr Gln Leu Gly Phe Gln 
        355                 360                 365             


Pro Leu Met Ser Asp Leu Gly Ser Thr Gly Ser Thr Glu Gly Ala 
    370                 375                 380             


<210> 16
<211> 383
<212> PRT
<213> Homo sapiens

<400> 16
Met Gly Leu Gly Arg Val Leu Leu Phe Leu Ala Val Ala Phe Pro Phe 
1               5                   10                  15      


Ala Pro Pro Ala Ala Ala Ala Glu Pro His Ser Leu Arg Tyr Asn Leu 
            20                  25                  30          


Met Val Leu Ser Gln Asp Gly Ser Val Gln Ser Gly Phe Leu Ala Glu 
        35                  40                  45              


Gly His Leu Asp Gly Gln Pro Phe Leu Arg Tyr Asp Arg Gln Lys Arg 
    50                  55                  60                  


Arg Ala Lys Pro Gln Gly Gln Trp Ala Glu Asn Val Leu Gly Ala Lys 
65                  70                  75                  80  


Thr Trp Asp Thr Glu Thr Glu Asp Leu Thr Glu Asn Gly Gln Asp Leu 
                85                  90                  95      


Arg Arg Thr Leu Thr His Ile Lys Asp Gln Lys Gly Gly Leu His Ser 
            100                 105                 110         


Leu Gln Glu Ile Arg Val Cys Glu Ile His Glu Asp Ser Ser Thr Arg 
        115                 120                 125             


Gly Ser Arg His Phe Tyr Tyr Asp Gly Glu Leu Phe Leu Ser Gln Asn 
    130                 135                 140                 


Leu Glu Thr Gln Glu Ser Thr Val Pro Gln Ser Ser Arg Ala Gln Thr 
145                 150                 155                 160 


Leu Ala Met Asn Val Thr Asn Phe Trp Lys Glu Asp Ala Met Lys Thr 
                165                 170                 175     


Lys Thr His Tyr Arg Ala Met Gln Ala Asp Cys Leu Gln Lys Leu Gln 
            180                 185                 190         


Arg Tyr Leu Lys Ser Gly Val Ala Ile Arg Arg Thr Val Pro Pro Met 
        195                 200                 205             


Val Asn Val Thr Cys Ser Glu Val Ser Glu Gly Asn Ile Thr Val Thr 
    210                 215                 220                 


Cys Arg Ala Ser Ser Phe Tyr Pro Arg Asn Ile Thr Leu Thr Trp Arg 
225                 230                 235                 240 


Gln Asp Gly Val Ser Leu Ser His Asn Thr Gln Gln Trp Gly Asp Val 
                245                 250                 255     


Leu Pro Asp Gly Asn Gly Thr Tyr Gln Thr Trp Val Ala Thr Arg Ile 
            260                 265                 270         


Arg Gln Gly Glu Glu Gln Arg Phe Thr Cys Tyr Met Glu His Ser Gly 
        275                 280                 285             


Asn His Gly Thr His Pro Val Pro Ser Gly Lys Ala Leu Val Leu Gln 
    290                 295                 300                 


Ser Gln Arg Thr Asp Phe Pro Tyr Val Ser Ala Ala Met Pro Cys Phe 
305                 310                 315                 320 


Val Ile Ile Ile Ile Leu Cys Val Pro Cys Cys Lys Lys Lys Thr Ser 
                325                 330                 335     


Ala Ala Glu Gly Pro Glu Leu Val Ser Leu Gln Val Leu Asp Gln His 
            340                 345                 350         


Pro Val Gly Thr Gly Asp His Arg Asp Ala Ala Gln Leu Gly Phe Gln 
        355                 360                 365             


Pro Leu Met Ser Ala Thr Gly Ser Thr Gly Ser Thr Glu Gly Thr 
    370                 375                 380             


<210> 17
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      linker peptide

<400> 17
Gly Gly Gly Gly Ser 
1               5   


<210> 18
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      6xHis tag

<400> 18
His His His His His His 
1               5       


<210> 19
<211> 351
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide


<220>
<221> CDS
<222> (1)..(348)

<400> 19
gac att gtg ctc acc cag act aca tcc tac ttg tct gta tct cta gga         48
Asp Ile Val Leu Thr Gln Thr Thr Ser Tyr Leu Ser Val Ser Leu Gly           
1               5                   10                  15                

ggc aga gtc acc att gct tgc aag gca agt gcc cac att aat aat tgg         96
Gly Arg Val Thr Ile Ala Cys Lys Ala Ser Ala His Ile Asn Asn Trp           
            20                  25                  30                    

tta gcc tgg tat cag cag aaa cca gga aat gct cct agg ctc tta ata        144
Leu Ala Trp Tyr Gln Gln Lys Pro Gly Asn Ala Pro Arg Leu Leu Ile           
        35                  40                  45                        

tct gat gca acc agt ttg gaa act ggg gtt cct tca aga ttc agt ggc        192
Ser Asp Ala Thr Ser Leu Glu Thr Gly Val Pro Ser Arg Phe Ser Gly           
    50                  55                  60                            

agt gga tct gga aag gat tac act ctc agc att acc agt ctt cag act        240
Ser Gly Ser Gly Lys Asp Tyr Thr Leu Ser Ile Thr Ser Leu Gln Thr           
65                  70                  75                  80            

gaa gat gtt gct act tat tac tgt caa cat tat tgg agt act ccg tgg        288
Glu Asp Val Ala Thr Tyr Tyr Cys Gln His Tyr Trp Ser Thr Pro Trp           
                85                  90                  95                

acg ttc ggt gga ggc acc aag ctg gaa atc aaa cgg gct gat gct gca        336
Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Ala Asp Ala Ala           
            100                 105                 110                   

cca act gta tcc acc                                                    351
Pro Thr Val Ser                                                           
        115                                                               


<210> 20
<211> 383
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide


<220>
<221> CDS
<222> (1)..(381)

<400> 20
ctt ccg gag gtc cag ctg gag gag tct gga cct ggc ctg gtg aaa ccc         48
Leu Pro Glu Val Gln Leu Glu Glu Ser Gly Pro Gly Leu Val Lys Pro           
1               5                   10                  15                

tct cag tct ctg tcc ctc acc tgc act gtc act ggc tac tca atc acc         96
Ser Gln Ser Leu Ser Leu Thr Cys Thr Val Thr Gly Tyr Ser Ile Thr           
            20                  25                  30                    

agt gat tat gcc tgg aac tgg atc cgg cag ttt cca gga aac aaa ctg        144
Ser Asp Tyr Ala Trp Asn Trp Ile Arg Gln Phe Pro Gly Asn Lys Leu           
        35                  40                  45                        

gag tgg atg ggc tac ata agc tac agt ggt agc act aac tac aac cca        192
Glu Trp Met Gly Tyr Ile Ser Tyr Ser Gly Ser Thr Asn Tyr Asn Pro           
    50                  55                  60                            

tct ctc aaa agt cga atc tct atc act cga gac acc tcc aag aac cag        240
Ser Leu Lys Ser Arg Ile Ser Ile Thr Arg Asp Thr Ser Lys Asn Gln           
65                  70                  75                  80            

ttc ttc ctg cag ttg aat tct gtg att act gag gac aca gcc aca tat        288
Phe Phe Leu Gln Leu Asn Ser Val Ile Thr Glu Asp Thr Ala Thr Tyr           
                85                  90                  95                

tac tgt gca agg ggg ggg act tac ttt gac tac tgg ggc caa ggc acc        336
Tyr Cys Ala Arg Gly Gly Thr Tyr Phe Asp Tyr Trp Gly Gln Gly Thr           
            100                 105                 110                   

act ctc aca gtc tcc tca gcc aaa acg aca ccc cca tct gtc tat tc         383
Thr Leu Thr Val Ser Ser Ala Lys Thr Thr Pro Pro Ser Val Tyr               
        115                 120                 125                       


