                         SEQUENCE LISTING

<110>  The Johns Hopkins University
 
<120>  RASAL1 IS A MAJOR TUMOR SUPPRESSOR GENE IN THYROID CANCER

<130>  P12597-02

<150>  61/861,822
<151>  2013-08-02

<160>  86    

<170>  PatentIn version 3.5

<210>  1
<211>  15
<212>  DNA
<213>  Homo sapiens

<400>  1
cgttctaact ccctg                                                        15


<210>  2
<211>  14
<212>  DNA
<213>  Homo sapiens

<400>  2
tggatccctg caag                                                         14


<210>  3
<211>  16
<212>  DNA
<213>  Homo sapiens

<400>  3
tcaaaggcgc actctc                                                       16


<210>  4
<211>  15
<212>  DNA
<213>  Homo sapiens

<400>  4
ctgcccgccc gccat                                                        15


<210>  5
<211>  15
<212>  DNA
<213>  Homo sapiens

<400>  5
gccatgcgcc tcgcc                                                        15


<210>  6
<211>  15
<212>  DNA
<213>  Homo sapiens

<400>  6
gccatgcgcc tcgcc                                                        15


<210>  7
<211>  14
<212>  DNA
<213>  Homo sapiens

<400>  7
cgattcttcg cacc                                                         14


<210>  8
<211>  15
<212>  DNA
<213>  Homo sapiens

<400>  8
acgtctggct cagcg                                                        15


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for RASAL1

<400>  9
gtggatgtgg atggggatga                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for RASAL1

<400>  10
ttcttgcact ggaggtaggt                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for DAB2IP

<400>  11
catggaggaa gaggtggtca                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for DAB2IP

<400>  12
aggtacttct tcttggctgg                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for NF1

<400>  13
tggcactgca agcaaatgga                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for NF1

<400>  14
taggccacgc tctgtgtatt                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for SPRED1

<400>  15
gatgactcaa gtggtggatg                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for SPRED1

<400>  16
cctctatcaa aagccctagc                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for SPRED2

<400>  17
tcatccatgg tgaacgacag                                                   20


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for SPRED2

<400>  18
atggatggtg gaagatgacg                                                   20


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for SPRY1

<400>  19
tccactgatt gccagaactc                                                   20


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for SPRY1

<400>  20
ttcttgtctt ggtgctgtcc                                                   20


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for SPRY2

<400>  21
ttggtgcaaa gccgcgatca                                                   20


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for SPRY2

<400>  22
ttggtgtttc ggatggctct                                                   20


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for RKIP

<400>  23
gcccacccag gttaagaata                                                   20


<210>  24
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for RKIP

<400>  24
ctcgtaaacc agccagacat                                                   20


<210>  25
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for DUSP5

<400>  25
cttcctcaaa gggggatatg                                                   20


<210>  26
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for DUSP5

<400>  26
tcgcacttgg atgcatggta                                                   20


<210>  27
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for DUSP6

<400>  27
agcgactgga acgagaatac                                                   20


<210>  28
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for DUSP6

<400>  28
cgatgtccga ggaagagtca                                                   20


<210>  29
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for TSC1

<400>  29
caacaagcaa atgtcgggga                                                   20


<210>  30
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for TSC1

<400>  30
aggatggata aacgagtggc                                                   20


<210>  31
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for TSC2

<400>  31
tgttggcttg tcctcggaat                                                   20


<210>  32
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for TSC2

<400>  32
caggcagttg tagcagacca                                                   20


<210>  33
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for LKB1

<400>  33
atggacacgt tcatccaccg                                                   20


<210>  34
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for LKB1

<400>  34
gccgtaacct cctcagtagt                                                   20


<210>  35
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MSP forward methylation primer for RASAL1

<400>  35
gtttttattt gtagagttcg gac                                               23


<210>  36
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MSP reverse methylation primer for RASAL1

<400>  36
atcccaatac cgcctatccg                                                   20


<210>  37
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MSP forward unmethylation primer for RASAL1

<400>  37
ggtttttatt tgtagagttt ggat                                              24


<210>  38
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MSP reverse unmethylation primer for RASAL1

<400>  38
aatcccaata ccacctatcc a                                                 21


<210>  39
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for RASAL1 Exon2

<400>  39
tgaagcaggt gacatgtaga cg                                                22


<210>  40
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for RASAL1 Exon2

<400>  40
gggcatctgc taactctagg c                                                 21


<210>  41
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for RASAL1 Exon 3

<400>  41
tatttctatg ggtggaagcc c                                                 21


<210>  42
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for RASAL1 Exon 3

<400>  42
aattcttggt cccagattcc c                                                 21


<210>  43
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for RASAL1 Exon 4

<400>  43
gtgtctgggt ctccaggtgt c                                                 21


<210>  44
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for RASAL1 Exon 4

<400>  44
acgtgaaggt ctgagtcagg g                                                 21


<210>  45
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for RASAL1 Exon 5

<400>  45
cacccagacc ttgacacctg                                                   20


<210>  46
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for RASAL1 Exon 5

<400>  46
tatgatagca ccaccgcact c                                                 21


<210>  47
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for RASAL1 Exon 6

<400>  47
caacagaacc agaccctgtg                                                   20


<210>  48
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for RASAL1 Exon 6

<400>  48
ggactgagga ggtcccaaac                                                   20


<210>  49
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for RASAL1 Exon 7

<400>  49
gtttggacgg tcatggttag g                                                 21


<210>  50
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for RASAL1 Exon 7

<400>  50
ggaagcgagt cttcttgatg g                                                 21


<210>  51
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for RASAL1 Exon 8

<400>  51
cagagcttgg agacctcagt g                                                 21


<210>  52
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for RASAL1 Exon 8

<400>  52
gctgtacatc cacccttctg a                                                 21


<210>  53
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for RASAL1 Exon 9

<400>  53
ctgttcttcc agggctaggt g                                                 21


<210>  54
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for RASAL1 Exon 9

<400>  54
ggtgaacggg tgtaaagtga ac                                                22


<210>  55
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for RASAL1 Exons 10-11

<400>  55
atcgtgttcg gccagatctt                                                   20


<210>  56
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for RASAL1 Exons 10-11

<400>  56
tcctgcaagc ccaccattga                                                   20


<210>  57
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for RASAL1 Exons 12-13

<400>  57
gttcagctcc ctaaatccca                                                   20


<210>  58
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for RASAL1 Exons 12-13

<400>  58
aggtcagggt cctcaggctt                                                   20


<210>  59
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for RASAL1 Exon 14

<400>  59
aagatgagtc ctctcggagc                                                   20


<210>  60
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for RASAL1 Exon 14

<400>  60
cagttctgtc ctgactcctc                                                   20


<210>  61
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for RASAL1 Exon 15

<400>  61
tgtggcttcc tggtcaattc                                                   20


<210>  62
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for RASAL1 Exon 15

<400>  62
gctctgctcc taccatgctt                                                   20


<210>  63
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for RASAL1 Exon 16

<400>  63
tgcccggcaa tatgagtctt                                                   20


<210>  64
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for RASAL1 Exon 16

<400>  64
gaagtccctg agtggtgtct                                                   20


<210>  65
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for RASAL1 Exon 17

<400>  65
ctaggggagc agagaaccat                                                   20


<210>  66
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for RASAL1 Exon 17

<400>  66
acaaagaggc agcacactga                                                   20


<210>  67
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for RASAL1 Exon 18

<400>  67
atggacgagc acacagagca                                                   20


<210>  68
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for RASAL1 Exon 18

<400>  68
ccatcgcggt ggggtctca                                                    19


<210>  69
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for RASAL1 Exon 19

<400>  69
aaggtgagac cccaccgcga                                                   20


<210>  70
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for RASAL1 Exon 19

<400>  70
ccacgagata ggcactgtta                                                   20


<210>  71
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for RASAL1 Exon 20

<400>  71
agtagtaggt ttgaggaggg                                                   20


<210>  72
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for RASAL1 Exon 20

<400>  72
atccaccaac acacatggga                                                   20


<210>  73
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for RASAL1 Exon 21

<400>  73
gtgatggtga tggttcagga                                                   20


<210>  74
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for RASAL1 Exon 21

<400>  74
tgggctcaag caattctca                                                    19


<210>  75
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for RASAL1 Exon 22

<400>  75
agcatgagaa accattgggt c                                                 21


<210>  76
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for RASAL1 Exon 22

<400>  76
gactaggcac gtctctggga g                                                 21


<210>  77
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutagenesis forward primer for RASAL1 N344S

<400>  77
ccctcttccg ttctagctcc ctggcatcc                                         29


<210>  78
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutagenesis reverse primer for RASAL1 N344S

<400>  78
ggatgccagg gagctagaac ggaagaggg                                         29


<210>  79
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutagenesis forward primer for RASAL1 P385S

<400>  79
acatggagct ggattcctgc aagatggacc                                        30


<210>  80
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutagenesis reverse primer for RASAL1 P385S

<400>  80
ggtccatctt gcaggaatcc agctccatgt                                        30


<210>  81
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutagenesis forward primer for RASAL1 R438C

<400>  81
ccgcccgcca tgtgcctcgc cttca                                             25


<210>  82
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutagenesis reverse primer for RASAL1 R438C

<400>  82
tgaaggcgag gcacatggcg ggcgg                                             25


<210>  83
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutagenesis forward primer for RASAL1 P474L

<400>  83
ctcttcttgc gattcttagc acctgccatc c                                      31


<210>  84
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutagenesis reverse primer for RASAL1 P474L

<400>  84
ggatggcagg tgctaagaat cgcaagaaga g                                      31


<210>  85
<211>  3841
<212>  DNA
<213>  Homo sapiens

<400>  85
gtgtttaact ggaaactaga acgagatgga aggggatgtt caaggcccct cccttgactc       60

tgaacggacc cccagggaac atgcgaccct ctctctggcg acgcctccca cccaccacta      120

atacttgctc ctggaccggg gggcgcggag gttggagaga ggaggcaggt gtctgcatgc      180

tacccgggtc tcggacaggc ggcactggga ccacgaggca gggagccagg cttgaagcag      240

gtgacatgta gacgtcccct ggtccagcct cggaacctga gcgcccttct gcctggaaag      300

tttgtggcta ggcgccatgg ccaagagcag ctccctgaat gttcgcgtgg tggagggccg      360

cgcgctgcct gccaaggacg tgtctgggag cagcgacccc tactgcctag tgaaagtgga      420

cgacgaggtg gtggccagga cagctactgt ctggaggagc ctgggcccct tctgggggga      480

ggagtacacg gtgcacctgc ctctggattt ccaccagctg gccttctacg tgctggatga      540

ggacactgtc gggcacgacg acatcatcgg caagatctcg ctgagcaggg aggcgattac      600

agccgacccc cgagggattg acagctggat taacttgagc cgagtggacc cagatgcaga      660

agtgcagggt gagatctgcc tgtcagtgca gatgctggag gatgggcagg gccgctgcct      720

tcgctgccat gtgcttcagg ccagggacct ggctcccaga gacatctctg gcacatctga      780

cccatttgca cgtgtgtttt ggggcagcca gagcttggag acctcaacca tcaagaagac      840

tcgcttcccg cactgggatg aagtgctgga gctgcgggag atgccaggtg ccccgtcccc      900

actgcgggtg gagctctggg actgggacat ggtgggcaag aatgacttct tgggcatggt      960

ggagttctct ccaaagaccc tccagcagaa gccacctaaa ggctggttcc gcctcctgcc     1020

ctttcccaga gccgaggagg attctggggg gaacctgggt gccctgcgag tgaaggtacg     1080

cctgattgag gaccgcgtcc tgccctccca gtgctaccag cctctcatgg agctgctcat     1140

ggagtctgtg caggggccag cagaggagga cactgctagc cccttggctt tgctggaaga     1200

gctgaccttg ggggactgcc gccaggacct tgccaccaag ctggtgaaac tctttcttgg     1260

ccggggactg gctgggcgct ttctggacta tctcacccgg cgtgaggtgg ctcggaccat     1320

ggaccccaac accctcttcc gttctaactc cctggcatcc aagtcgatgg aacagtttat     1380

gaagctcgtg ggcatgccct acctgcacga ggtcctgaag cctgtgatta gccgtgtctt     1440

tgaggagaag aagtacatgg agctggatcc ctgcaagatg gacctgggcc gcacccggag     1500

gatctccttc aaaggcgcac tctcggagga gcagatgcgg gagaccagcc tggggctgct     1560

gacgggctac ctggggccca tcgtggacgc catcgtgggc tccgtggggc gctgcccgcc     1620

cgccatgcgc ctcgccttca agcagctgca ccggcgagtg gaggagcgct tcccccaggc     1680

cgagcaccag gatgtgaagt acctggccat cagtggattt ctcttcttgc gattcttcgc     1740

acctgccatc cttaccccaa agctgtttga ccttcgggac caacacgcgg acccccagac     1800

tagccgctca ctgctgttgc ttgccaaggc tgtgcagagc attggaaacc tgggccagca     1860

gctgggccaa ggcaaggaac tgtggatggc ccccctgcac cccttcctgc tgcagtgtgt     1920

ctcacgtgtg agagacttcc tggaccggct ggtggatgtg gatggggatg aagctggtgt     1980

cccagccagg gccctgttcc cgccctcggc cattgttcga gaaggctatc tgctgaagcg     2040

caaggaggag cctgccggcc tggccacgcg ctttgccttc aagaagcgct acgtctggct     2100

cagcggggag accctctcct tctccaagag tcctgagtgg cagatgtgtc actccatccc     2160

cgtgtctcac atccgcgccg tggagcgcgt agacgagggc gccttccaac tgccccacgt     2220

gatgcaggtg gtgacgcagg acggcacggg ggcgctgcac accacctacc tccagtgcaa     2280

gaatgtgaat gagctcaacc agtggctctc ggccttgcgc aaggccagcg cccccaaccc     2340

gaacaagctg gccgcctgcc accccggtgc cttccgcagc gcgcgctgga cctgctgcct     2400

ccaggctgag cgctcagccg ccggctgcag ccgtacacac tcagctgtca ccctggggga     2460

ctggagtgac ccactggatc ctgatgctga ggcccagaca gtgtatcggc agctgctcct     2520

ggggcgggac cagctcaggc tgaaattact ggaggattct aacatggata caactctgga     2580

ggcagacaca ggggcctgtc ctgaggtcct ggcccggcaa agagcagcaa ctgcccgcct     2640

gctggaggtg ctcgcagacc tggatcgtgc ccacgaggag ttccagcagc aggagcgagg     2700

gaaggcggcc ctgggccccc ttggccccta aggaaatgcc agagctagcc cggaaggagg     2760

agcaagagcc agggggccct cttcagcgca tcctgccccg ggagtctcct gtctccttgg     2820

acctctttga ttctgtggtt tggaggctcc cagagacgtg cctagtcctg tgtgccttga     2880

gtccagaact cagggcatgg aagccctttg gcaggggcca gccttgcact gagtgaaact     2940

tgccctctgg cttgattcag actggagtgg ataggataag gaacctgact tatttgactg     3000

agactggggt ctctacttca ccaaactggc ctctatccat accaaggagg ccagcctggc     3060

cctgagctgc tggatacagc tggacctgaa ttcctgatgc ccatgtgatg ttgttgcccc     3120

agatgggcac taaatggcct cactccttcc tgttttcatg tctgctaatc cctataacct     3180

cactgattct tctgtaccct gcccttggcc taggactcca accacaagct tccagaatca     3240

ggtgccctca ggaagaacca aggctgggtg ggggtccagt gtgccaaact cagacccttg     3300

gagcctggga gaccttgggc caggctgttt atctctctct gggtctcaga ttaccctgta     3360

taaaaagagg agggaaagtc tagatgtgtg gttttcaaac tggattccaa tgaggtagtt     3420

caagaacaag ggaggagttt ctctgactgt gggccaaaga ccacccgcta gaatgtctgc     3480

tagaaatgca gatttctgag gcccagccag ctactgactc agaatctgag ggtatatgaa     3540

ggcaagaatc agggttattt aacagattcc gtaggtcatt tggatgagta tcagctttga     3600

ggaccacagg cctggggaat gggcaatttt attttatttt gtttttagag ttgggggtct     3660

cactctgttg cccaggctgc agtacagtgg catgatcata ggtcagtgca ccctcaaact     3720

tctggacttc agtgatcctc ccacgttagc ctccagagca gctgcgacta caggtgcaag     3780

ccatcacgcc tggctaattt ttaaattttt gtagagatgg ggggtctcac tatgttgccc     3840

a                                                                     3841


<210>  86
<211>  804
<212>  PRT
<213>  Homo sapiens

<400>  86

Met Ala Lys Ser Ser Ser Leu Asn Val Arg Val Val Glu Gly Arg Ala 
1               5                   10                  15      


Leu Pro Ala Lys Asp Val Ser Gly Ser Ser Asp Pro Tyr Cys Leu Val 
            20                  25                  30          


Lys Val Asp Asp Glu Val Val Ala Arg Thr Ala Thr Val Trp Arg Ser 
        35                  40                  45              


Leu Gly Pro Phe Trp Gly Glu Glu Tyr Thr Val His Leu Pro Leu Asp 
    50                  55                  60                  


Phe His Gln Leu Ala Phe Tyr Val Leu Asp Glu Asp Thr Val Gly His 
65                  70                  75                  80  


Asp Asp Ile Ile Gly Lys Ile Ser Leu Ser Arg Glu Ala Ile Thr Ala 
                85                  90                  95      


Asp Pro Arg Gly Ile Asp Ser Trp Ile Asn Leu Ser Arg Val Asp Pro 
            100                 105                 110         


Asp Ala Glu Val Gln Gly Glu Ile Cys Leu Ser Val Gln Met Leu Glu 
        115                 120                 125             


Asp Gly Gln Gly Arg Cys Leu Arg Cys His Val Leu Gln Ala Arg Asp 
    130                 135                 140                 


Leu Ala Pro Arg Asp Ile Ser Gly Thr Ser Asp Pro Phe Ala Arg Val 
145                 150                 155                 160 


Phe Trp Gly Ser Gln Ser Leu Glu Thr Ser Thr Ile Lys Lys Thr Arg 
                165                 170                 175     


Phe Pro His Trp Asp Glu Val Leu Glu Leu Arg Glu Met Pro Gly Ala 
            180                 185                 190         


Pro Ser Pro Leu Arg Val Glu Leu Trp Asp Trp Asp Met Val Gly Lys 
        195                 200                 205             


Asn Asp Phe Leu Gly Met Val Glu Phe Ser Pro Lys Thr Leu Gln Gln 
    210                 215                 220                 


Lys Pro Pro Lys Gly Trp Phe Arg Leu Leu Pro Phe Pro Arg Ala Glu 
225                 230                 235                 240 


Glu Asp Ser Gly Gly Asn Leu Gly Ala Leu Arg Val Lys Val Arg Leu 
                245                 250                 255     


Ile Glu Asp Arg Val Leu Pro Ser Gln Cys Tyr Gln Pro Leu Met Glu 
            260                 265                 270         


Leu Leu Met Glu Ser Val Gln Gly Pro Ala Glu Glu Asp Thr Ala Ser 
        275                 280                 285             


Pro Leu Ala Leu Leu Glu Glu Leu Thr Leu Gly Asp Cys Arg Gln Asp 
    290                 295                 300                 


Leu Ala Thr Lys Leu Val Lys Leu Phe Leu Gly Arg Gly Leu Ala Gly 
305                 310                 315                 320 


Arg Phe Leu Asp Tyr Leu Thr Arg Arg Glu Val Ala Arg Thr Met Asp 
                325                 330                 335     


Pro Asn Thr Leu Phe Arg Ser Asn Ser Leu Ala Ser Lys Ser Met Glu 
            340                 345                 350         


Gln Phe Met Lys Leu Val Gly Met Pro Tyr Leu His Glu Val Leu Lys 
        355                 360                 365             


Pro Val Ile Ser Arg Val Phe Glu Glu Lys Lys Tyr Met Glu Leu Asp 
    370                 375                 380                 


Pro Cys Lys Met Asp Leu Gly Arg Thr Arg Arg Ile Ser Phe Lys Gly 
385                 390                 395                 400 


Ala Leu Ser Glu Glu Gln Met Arg Glu Thr Ser Leu Gly Leu Leu Thr 
                405                 410                 415     


Gly Tyr Leu Gly Pro Ile Val Asp Ala Ile Val Gly Ser Val Gly Arg 
            420                 425                 430         


Cys Pro Pro Ala Met Arg Leu Ala Phe Lys Gln Leu His Arg Arg Val 
        435                 440                 445             


Glu Glu Arg Phe Pro Gln Ala Glu His Gln Asp Val Lys Tyr Leu Ala 
    450                 455                 460                 


Ile Ser Gly Phe Leu Phe Leu Arg Phe Phe Ala Pro Ala Ile Leu Thr 
465                 470                 475                 480 


Pro Lys Leu Phe Asp Leu Arg Asp Gln His Ala Asp Pro Gln Thr Ser 
                485                 490                 495     


Arg Ser Leu Leu Leu Leu Ala Lys Ala Val Gln Ser Ile Gly Asn Leu 
            500                 505                 510         


Gly Gln Gln Leu Gly Gln Gly Lys Glu Leu Trp Met Ala Pro Leu His 
        515                 520                 525             


Pro Phe Leu Leu Gln Cys Val Ser Arg Val Arg Asp Phe Leu Asp Arg 
    530                 535                 540                 


Leu Val Asp Val Asp Gly Asp Glu Ala Gly Val Pro Ala Arg Ala Leu 
545                 550                 555                 560 


Phe Pro Pro Ser Ala Ile Val Arg Glu Gly Tyr Leu Leu Lys Arg Lys 
                565                 570                 575     


Glu Glu Pro Ala Gly Leu Ala Thr Arg Phe Ala Phe Lys Lys Arg Tyr 
            580                 585                 590         


Val Trp Leu Ser Gly Glu Thr Leu Ser Phe Ser Lys Ser Pro Glu Trp 
        595                 600                 605             


Gln Met Cys His Ser Ile Pro Val Ser His Ile Arg Ala Val Glu Arg 
    610                 615                 620                 


Val Asp Glu Gly Ala Phe Gln Leu Pro His Val Met Gln Val Val Thr 
625                 630                 635                 640 


Gln Asp Gly Thr Gly Ala Leu His Thr Thr Tyr Leu Gln Cys Lys Asn 
                645                 650                 655     


Val Asn Glu Leu Asn Gln Trp Leu Ser Ala Leu Arg Lys Ala Ser Ala 
            660                 665                 670         


Pro Asn Pro Asn Lys Leu Ala Ala Cys His Pro Gly Ala Phe Arg Ser 
        675                 680                 685             


Ala Arg Trp Thr Cys Cys Leu Gln Ala Glu Arg Ser Ala Ala Gly Cys 
    690                 695                 700                 


Ser Arg Thr His Ser Ala Val Thr Leu Gly Asp Trp Ser Asp Pro Leu 
705                 710                 715                 720 


Asp Pro Asp Ala Glu Ala Gln Thr Val Tyr Arg Gln Leu Leu Leu Gly 
                725                 730                 735     


Arg Asp Gln Leu Arg Leu Lys Leu Leu Glu Asp Ser Asn Met Asp Thr 
            740                 745                 750         


Thr Leu Glu Ala Asp Thr Gly Ala Cys Pro Glu Val Leu Ala Arg Gln 
        755                 760                 765             


Arg Ala Ala Thr Ala Arg Leu Leu Glu Val Leu Ala Asp Leu Asp Arg 
    770                 775                 780                 


Ala His Glu Glu Phe Gln Gln Gln Glu Arg Gly Lys Ala Ala Leu Gly 
785                 790                 795                 800 


Pro Leu Gly Pro 
                


