                         SEQUENCE LISTING

<110>  Novozymes A/S
 
<120>  METHOD FOR PRODUCING A COFFEE EXTRACT

<130>  12671-WO-PCT

<160>  15    

<170>  PatentIn version 3.5

<210>  1
<211>  771
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic gene


<220>
<221>  CDS
<222>  (1)..(768)

<400>  1
atg att tcc gtc gct ttg gtc cca ctc ttc atc gct gtg ctt gcc agc         48
Met Ile Ser Val Ala Leu Val Pro Leu Phe Ile Ala Val Leu Ala Ser           
1               5                   10                  15                

gcg acg tcg agc gtc gtc ctc ccg acg aac tcg ttc agc tcg tac agc         96
Ala Thr Ser Ser Val Val Leu Pro Thr Asn Ser Phe Ser Ser Tyr Ser           
            20                  25                  30                    

gcc ttt gag cag cac tgg aac tac ctc tac cct tgg ggc tcg gac cac        144
Ala Phe Glu Gln His Trp Asn Tyr Leu Tyr Pro Trp Gly Ser Asp His           
        35                  40                  45                        

aac ggc tcc ggg cgc atg gtg ggc agc tcg tcg aac cac acg tac atc        192
Asn Gly Ser Gly Arg Met Val Gly Ser Ser Ser Asn His Thr Tyr Ile           
    50                  55                  60                            

agc gtc gcg gac aac gtt ctc acg ctc acc tcg aag ccc gtc tcc ggg        240
Ser Val Ala Asp Asn Val Leu Thr Leu Thr Ser Lys Pro Val Ser Gly           
65                  70                  75                  80            

cag cct ccg agc acc tcc aac ccg cac ccg gcc atc cat tac ttc tct        288
Gln Pro Pro Ser Thr Ser Asn Pro His Pro Ala Ile His Tyr Phe Ser           
                85                  90                  95                

ggc act gtc cat gcg aag caa cag gtc aag gtc gac gga agt agc gtc        336
Gly Thr Val His Ala Lys Gln Gln Val Lys Val Asp Gly Ser Ser Val           
            100                 105                 110                   

acg ggg ttc gac atc cag gga gag ttc att gct ccg act gca aaa ggc        384
Thr Gly Phe Asp Ile Gln Gly Glu Phe Ile Ala Pro Thr Ala Lys Gly           
        115                 120                 125                       

acg tgg cct gcg ttc tgg ctt act gcg gtg aat gga tgg cct ccg gag        432
Thr Trp Pro Ala Phe Trp Leu Thr Ala Val Asn Gly Trp Pro Pro Glu           
    130                 135                 140                           

agc gac att ggt gaa tgg aag ggc acc cag gaa aac tgg ttc aat acc        480
Ser Asp Ile Gly Glu Trp Lys Gly Thr Gln Glu Asn Trp Phe Asn Thr           
145                 150                 155                 160           

ttc aac act tcc tca tca gtc gcg acg aag cgc gtc gcc tgg ccc acg        528
Phe Asn Thr Ser Ser Ser Val Ala Thr Lys Arg Val Ala Trp Pro Thr           
                165                 170                 175               

gac ggc cag ttc cat tcc ctg aag gcc gag ctg cgc acg atc tcg ggc        576
Asp Gly Gln Phe His Ser Leu Lys Ala Glu Leu Arg Thr Ile Ser Gly           
            180                 185                 190                   

aac acg aag gac ctc tcg atc aag tac tac ttc gac gga acg ctg cag        624
Asn Thr Lys Asp Leu Ser Ile Lys Tyr Tyr Phe Asp Gly Thr Leu Gln           
        195                 200                 205                       

gcg act cac acg gca gct aac ttc cgc aac gcc gct atg tgg ttg att        672
Ala Thr His Thr Ala Ala Asn Phe Arg Asn Ala Ala Met Trp Leu Ile           
    210                 215                 220                           

gtc gac ctt cag atg gag gga agc tcg ggc tct ccg ggc cca gct ggc        720
Val Asp Leu Gln Met Glu Gly Ser Ser Gly Ser Pro Gly Pro Ala Gly           
225                 230                 235                 240           

ggc acg acg ttc caa atc agg aac gtc cag ttg acg aag tat acg ccg        768
Gly Thr Thr Phe Gln Ile Arg Asn Val Gln Leu Thr Lys Tyr Thr Pro           
                245                 250                 255               

tga                                                                    771


<210>  2
<211>  256
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  2

Met Ile Ser Val Ala Leu Val Pro Leu Phe Ile Ala Val Leu Ala Ser 
1               5                   10                  15      


Ala Thr Ser Ser Val Val Leu Pro Thr Asn Ser Phe Ser Ser Tyr Ser 
            20                  25                  30          


Ala Phe Glu Gln His Trp Asn Tyr Leu Tyr Pro Trp Gly Ser Asp His 
        35                  40                  45              


Asn Gly Ser Gly Arg Met Val Gly Ser Ser Ser Asn His Thr Tyr Ile 
    50                  55                  60                  


Ser Val Ala Asp Asn Val Leu Thr Leu Thr Ser Lys Pro Val Ser Gly 
65                  70                  75                  80  


Gln Pro Pro Ser Thr Ser Asn Pro His Pro Ala Ile His Tyr Phe Ser 
                85                  90                  95      


Gly Thr Val His Ala Lys Gln Gln Val Lys Val Asp Gly Ser Ser Val 
            100                 105                 110         


Thr Gly Phe Asp Ile Gln Gly Glu Phe Ile Ala Pro Thr Ala Lys Gly 
        115                 120                 125             


Thr Trp Pro Ala Phe Trp Leu Thr Ala Val Asn Gly Trp Pro Pro Glu 
    130                 135                 140                 


Ser Asp Ile Gly Glu Trp Lys Gly Thr Gln Glu Asn Trp Phe Asn Thr 
145                 150                 155                 160 


Phe Asn Thr Ser Ser Ser Val Ala Thr Lys Arg Val Ala Trp Pro Thr 
                165                 170                 175     


Asp Gly Gln Phe His Ser Leu Lys Ala Glu Leu Arg Thr Ile Ser Gly 
            180                 185                 190         


Asn Thr Lys Asp Leu Ser Ile Lys Tyr Tyr Phe Asp Gly Thr Leu Gln 
        195                 200                 205             


Ala Thr His Thr Ala Ala Asn Phe Arg Asn Ala Ala Met Trp Leu Ile 
    210                 215                 220                 


Val Asp Leu Gln Met Glu Gly Ser Ser Gly Ser Pro Gly Pro Ala Gly 
225                 230                 235                 240 


Gly Thr Thr Phe Gln Ile Arg Asn Val Gln Leu Thr Lys Tyr Thr Pro 
                245                 250                 255     


<210>  3
<211>  777
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic gene


<220>
<221>  CDS
<222>  (1)..(774)

<400>  3
atg ttt aat tct ata ccc cat ttc ctg cta atg acc tct ggg gtc gga         48
Met Phe Asn Ser Ile Pro His Phe Leu Leu Met Thr Ser Gly Val Gly           
1               5                   10                  15                

att gcg ttg atg cca caa gtg atg gca gtc agg acc atg ata cca tgc         96
Ile Ala Leu Met Pro Gln Val Met Ala Val Arg Thr Met Ile Pro Cys           
            20                  25                  30                    

acc agc ttc gac aac cag tcc aac ttc gac gca gac tgg aat tac aac        144
Thr Ser Phe Asp Asn Gln Ser Asn Phe Asp Ala Asp Trp Asn Tyr Asn           
        35                  40                  45                        

tac ccc tgg gga acc gac cac aac ggc gcc gcg cgt atg gat ggt gcc        192
Tyr Pro Trp Gly Thr Asp His Asn Gly Ala Ala Arg Met Asp Gly Ala           
    50                  55                  60                            

aac gcg gtc cgg ttg gcg agc aga ggt gac cgt aca ctt gtc att act        240
Asn Ala Val Arg Leu Ala Ser Arg Gly Asp Arg Thr Leu Val Ile Thr           
65                  70                  75                  80            

gca cgc cgt gcc gaa ggc ctg ccg cct gcc act cac gga ggc cag cag        288
Ala Arg Arg Ala Glu Gly Leu Pro Pro Ala Thr His Gly Gly Gln Gln           
                85                  90                  95                

att cct atc agg tat ctg tct ggg gcc atc cac gcc aaa gaa cag ttt        336
Ile Pro Ile Arg Tyr Leu Ser Gly Ala Ile His Ala Lys Glu Gln Phe           
            100                 105                 110                   

act gtc cgg cca aac ggc ggc tac gat ttc aca gga gag ttc aaa gcc        384
Thr Val Arg Pro Asn Gly Gly Tyr Asp Phe Thr Gly Glu Phe Lys Ala           
        115                 120                 125                       

aca aca acc aag ggt acc tgg cca gcc ttc tgg ctc aca gga gtg gac        432
Thr Thr Thr Lys Gly Thr Trp Pro Ala Phe Trp Leu Thr Gly Val Asp           
    130                 135                 140                           

agt tgg cca ccg gag att gac atg gca gaa tgg aaa ggt agc ggg aaa        480
Ser Trp Pro Pro Glu Ile Asp Met Ala Glu Trp Lys Gly Ser Gly Lys           
145                 150                 155                 160           

atc agc ttc aac acg ttc aac acg tcg agc gaa gtc gtg tcg cga gac        528
Ile Ser Phe Asn Thr Phe Asn Thr Ser Ser Glu Val Val Ser Arg Asp           
                165                 170                 175               

gtg gat tac cct gcg cct gac cag ttc cat agc atc aag tgc gaa gtg        576
Val Asp Tyr Pro Ala Pro Asp Gln Phe His Ser Ile Lys Cys Glu Val           
            180                 185                 190                   

aga gac gaa ggg cag gat gtc cgc gcc aat ttc tat atg gat ggc aac        624
Arg Asp Glu Gly Gln Asp Val Arg Ala Asn Phe Tyr Met Asp Gly Asn           
        195                 200                 205                       

ctg ctt acc act cag atc gga aaa gga tac gtc ggg aaa cca ctc ttc        672
Leu Leu Thr Thr Gln Ile Gly Lys Gly Tyr Val Gly Lys Pro Leu Phe           
    210                 215                 220                           

ttg att atc aac ttg cag atg gag gga tct tcg ggc acc ccg ggc ccc        720
Leu Ile Ile Asn Leu Gln Met Glu Gly Ser Ser Gly Thr Pro Gly Pro           
225                 230                 235                 240           

gag tca gat acc gaa tac gcc ata cga aac ctg gag gtt ctt tcc tat        768
Glu Ser Asp Thr Glu Tyr Ala Ile Arg Asn Leu Glu Val Leu Ser Tyr           
                245                 250                 255               

aac ccg tga                                                            777
Asn Pro                                                                   
                                                                          


<210>  4
<211>  258
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  4

Met Phe Asn Ser Ile Pro His Phe Leu Leu Met Thr Ser Gly Val Gly 
1               5                   10                  15      


Ile Ala Leu Met Pro Gln Val Met Ala Val Arg Thr Met Ile Pro Cys 
            20                  25                  30          


Thr Ser Phe Asp Asn Gln Ser Asn Phe Asp Ala Asp Trp Asn Tyr Asn 
        35                  40                  45              


Tyr Pro Trp Gly Thr Asp His Asn Gly Ala Ala Arg Met Asp Gly Ala 
    50                  55                  60                  


Asn Ala Val Arg Leu Ala Ser Arg Gly Asp Arg Thr Leu Val Ile Thr 
65                  70                  75                  80  


Ala Arg Arg Ala Glu Gly Leu Pro Pro Ala Thr His Gly Gly Gln Gln 
                85                  90                  95      


Ile Pro Ile Arg Tyr Leu Ser Gly Ala Ile His Ala Lys Glu Gln Phe 
            100                 105                 110         


Thr Val Arg Pro Asn Gly Gly Tyr Asp Phe Thr Gly Glu Phe Lys Ala 
        115                 120                 125             


Thr Thr Thr Lys Gly Thr Trp Pro Ala Phe Trp Leu Thr Gly Val Asp 
    130                 135                 140                 


Ser Trp Pro Pro Glu Ile Asp Met Ala Glu Trp Lys Gly Ser Gly Lys 
145                 150                 155                 160 


Ile Ser Phe Asn Thr Phe Asn Thr Ser Ser Glu Val Val Ser Arg Asp 
                165                 170                 175     


Val Asp Tyr Pro Ala Pro Asp Gln Phe His Ser Ile Lys Cys Glu Val 
            180                 185                 190         


Arg Asp Glu Gly Gln Asp Val Arg Ala Asn Phe Tyr Met Asp Gly Asn 
        195                 200                 205             


Leu Leu Thr Thr Gln Ile Gly Lys Gly Tyr Val Gly Lys Pro Leu Phe 
    210                 215                 220                 


Leu Ile Ile Asn Leu Gln Met Glu Gly Ser Ser Gly Thr Pro Gly Pro 
225                 230                 235                 240 


Glu Ser Asp Thr Glu Tyr Ala Ile Arg Asn Leu Glu Val Leu Ser Tyr 
                245                 250                 255     


Asn Pro 
        


<210>  5
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  5
acacaactgg ggatccacca tgaggaggtc cttccaacaa ccc                         43


<210>  6
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  6
ccctctagat ctcgagtgga catcttgaag ggacaactcc t                           41


<210>  7
<211>  1061
<212>  DNA
<213>  Neurospora crassa


<220>
<221>  exon
<222>  (1)..(710)

<220>
<221>  CDS
<222>  (1)..(710)

<220>
<221>  Intron
<222>  (711)..(840)

<220>
<221>  exon
<222>  (841)..(893)

<220>
<221>  CDS
<222>  (841)..(893)

<220>
<221>  Intron
<222>  (894)..(990)

<220>
<221>  exon
<222>  (991)..(1025)

<220>
<221>  CDS
<222>  (991)..(1022)

<220>
<221>  3'UTR
<222>  (1026)..(1061)

<400>  7
atg agg agg tcc ttc caa caa ccc ctt ccc ctt ctc ctc caa acc atc         48
Met Arg Arg Ser Phe Gln Gln Pro Leu Pro Leu Leu Leu Gln Thr Ile           
1               5                   10                  15                

acc atc ctc tgc ctc ttc cca tct acc ctc gcc tca tcc aag acc ctc         96
Thr Ile Leu Cys Leu Phe Pro Ser Thr Leu Ala Ser Ser Lys Thr Leu           
            20                  25                  30                    

cta atc cct tcc aca tcc ttc aac tcc acc acc acc ttc aac acc tac        144
Leu Ile Pro Ser Thr Ser Phe Asn Ser Thr Thr Thr Phe Asn Thr Tyr           
        35                  40                  45                        

tgg tcc ttc aat tac ccc tgg ggc acc gac cac aat ggc gcc gcg cgc        192
Trp Ser Phe Asn Tyr Pro Trp Gly Thr Asp His Asn Gly Ala Ala Arg           
    50                  55                  60                            

atg tcc cct tcc caa gtc tcc ata gcc ccc tct gct gac ggc acc tca        240
Met Ser Pro Ser Gln Val Ser Ile Ala Pro Ser Ala Asp Gly Thr Ser           
65                  70                  75                  80            

agc acc ctc act cta acc gcc cac cgc gtc acc ggc caa aaa ccc gct        288
Ser Thr Leu Thr Leu Thr Ala His Arg Val Thr Gly Gln Lys Pro Ala           
                85                  90                  95                

acc cac ggc ggc aaa cag atc ccc atc aag tac cta tcc ggc gcc atc        336
Thr His Gly Gly Lys Gln Ile Pro Ile Lys Tyr Leu Ser Gly Ala Ile           
            100                 105                 110                   

cac gcc aag cag cac ttc acc att act tct tct tct tct ggt gct ggt        384
His Ala Lys Gln His Phe Thr Ile Thr Ser Ser Ser Ser Gly Ala Gly           
        115                 120                 125                       

gca gtg tca ggc tac gat ttc tca gcc gag ttc cgc gcc ccc gtt gcg        432
Ala Val Ser Gly Tyr Asp Phe Ser Ala Glu Phe Arg Ala Pro Val Ala           
    130                 135                 140                           

aag ggg acc tgg cct gcg ttt tgg ttg acg gcc gtc aat ggg tgg ccg        480
Lys Gly Thr Trp Pro Ala Phe Trp Leu Thr Ala Val Asn Gly Trp Pro           
145                 150                 155                 160           

ccc gag att gat atg gcg gag tgg aag ggg agc ggc aag att agc ttt        528
Pro Glu Ile Asp Met Ala Glu Trp Lys Gly Ser Gly Lys Ile Ser Phe           
                165                 170                 175               

aac acg ttc aat act agt agt cag gtc atg gct cgg gat gtg gtt tat        576
Asn Thr Phe Asn Thr Ser Ser Gln Val Met Ala Arg Asp Val Val Tyr           
            180                 185                 190                   

ggc ccg aac ggg agt gag aag gag tgg cat agg gtt gtg tgt gag atc        624
Gly Pro Asn Gly Ser Glu Lys Glu Trp His Arg Val Val Cys Glu Ile           
        195                 200                 205                       

agg agg gat gga agg aat ggt gga aag gat gtg gag gtg agg ttt cgg        672
Arg Arg Asp Gly Arg Asn Gly Gly Lys Asp Val Glu Val Arg Phe Arg           
    210                 215                 220                           

atg gat ggg cag cta gtg gta acg cag tgg ggg aag gg  gtatgttggg         720
Met Asp Gly Gln Leu Val Val Thr Gln Trp Gly Lys Gly                       
225                 230                 235                               

cagccacttt atttgtgagt ttaaaccttt tttttttcct ttttttttct tcttcttctt      780

tcgttgtcgg ggtgaggttt ttcgcgttgt cggttgctaa ctggaagggt gatattgcag      840

g att atc aat ctc cag atg gaa ggg tcg tcg ggt tca cct ggt cct gaa      889
  Ile Ile Asn Leu Gln Met Glu Gly Ser Ser Gly Ser Pro Gly Pro Glu         
          240                 245                 250                     

gcg g gtaagtcaac tataggtctc ttcgttttgg tgatggaaga acctgatcgt           943
Ala                                                                       
                                                                          

ctcagccggt ccatttgctg acagtgatac agacactttg tatcaag tc  cga aat        998
                                                    Val Arg Asn           
                                                    255                   

ctt gag gtc tgg agc tat ggt gac tga gccgtgagca gaggagttgt             1045
Leu Glu Val Trp Ser Tyr Gly Asp                                           
        260                 265                                           

cccttcaaga tgtcca                                                     1061


<210>  8
<211>  265
<212>  PRT
<213>  Neurospora crassa

<400>  8

Met Arg Arg Ser Phe Gln Gln Pro Leu Pro Leu Leu Leu Gln Thr Ile 
1               5                   10                  15      


Thr Ile Leu Cys Leu Phe Pro Ser Thr Leu Ala Ser Ser Lys Thr Leu 
            20                  25                  30          


Leu Ile Pro Ser Thr Ser Phe Asn Ser Thr Thr Thr Phe Asn Thr Tyr 
        35                  40                  45              


Trp Ser Phe Asn Tyr Pro Trp Gly Thr Asp His Asn Gly Ala Ala Arg 
    50                  55                  60                  


Met Ser Pro Ser Gln Val Ser Ile Ala Pro Ser Ala Asp Gly Thr Ser 
65                  70                  75                  80  


Ser Thr Leu Thr Leu Thr Ala His Arg Val Thr Gly Gln Lys Pro Ala 
                85                  90                  95      


Thr His Gly Gly Lys Gln Ile Pro Ile Lys Tyr Leu Ser Gly Ala Ile 
            100                 105                 110         


His Ala Lys Gln His Phe Thr Ile Thr Ser Ser Ser Ser Gly Ala Gly 
        115                 120                 125             


Ala Val Ser Gly Tyr Asp Phe Ser Ala Glu Phe Arg Ala Pro Val Ala 
    130                 135                 140                 


Lys Gly Thr Trp Pro Ala Phe Trp Leu Thr Ala Val Asn Gly Trp Pro 
145                 150                 155                 160 


Pro Glu Ile Asp Met Ala Glu Trp Lys Gly Ser Gly Lys Ile Ser Phe 
                165                 170                 175     


Asn Thr Phe Asn Thr Ser Ser Gln Val Met Ala Arg Asp Val Val Tyr 
            180                 185                 190         


Gly Pro Asn Gly Ser Glu Lys Glu Trp His Arg Val Val Cys Glu Ile 
        195                 200                 205             


Arg Arg Asp Gly Arg Asn Gly Gly Lys Asp Val Glu Val Arg Phe Arg 
    210                 215                 220                 


Met Asp Gly Gln Leu Val Val Thr Gln Trp Gly Lys Gly Ile Ile Asn 
225                 230                 235                 240 


Leu Gln Met Glu Gly Ser Ser Gly Ser Pro Gly Pro Glu Ala Val Arg 
                245                 250                 255     


Asn Leu Glu Val Trp Ser Tyr Gly Asp 
            260                 265 


<210>  9
<211>  1347
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic gene


<220>
<221>  CDS
<222>  (1)..(1344)

<400>  9
atg ttg tgg ttg cgc aag gtg atc cct gtt ttc ctc tct ctg gtc gtt         48
Met Leu Trp Leu Arg Lys Val Ile Pro Val Phe Leu Ser Leu Val Val           
1               5                   10                  15                

gcg gcc cgc gcc gag aca cag atc gtc tct ggt gct gct tgg aca gac         96
Ala Ala Arg Ala Glu Thr Gln Ile Val Ser Gly Ala Ala Trp Thr Asp           
            20                  25                  30                    

aca agc gga aac gtc att cag gcc cat ggc gct gga atc ttg aag gtc        144
Thr Ser Gly Asn Val Ile Gln Ala His Gly Ala Gly Ile Leu Lys Val           
        35                  40                  45                        

gga agc acg ttt tac tgg ttc ggt gaa gac aag acg gag aac agt gcc        192
Gly Ser Thr Phe Tyr Trp Phe Gly Glu Asp Lys Thr Glu Asn Ser Ala           
    50                  55                  60                            

ttg ttc cac gca gtg tcg tgc tat aca tct acg gac ctg acg aac tgg        240
Leu Phe His Ala Val Ser Cys Tyr Thr Ser Thr Asp Leu Thr Asn Trp           
65                  70                  75                  80            

act cgt caa agc aat gcg ctt tct ccg gtc gcc aac acc atg att tcc        288
Thr Arg Gln Ser Asn Ala Leu Ser Pro Val Ala Asn Thr Met Ile Ser           
                85                  90                  95                

tct aac aac atc gtt gag cgt ccc aaa gtt tta ttc aac aag aag aat        336
Ser Asn Asn Ile Val Glu Arg Pro Lys Val Leu Phe Asn Lys Lys Asn           
            100                 105                 110                   

caa gaa tac gtg atg tgg ttc cat tct gat agt tcc aac tat gga gct        384
Gln Glu Tyr Val Met Trp Phe His Ser Asp Ser Ser Asn Tyr Gly Ala           
        115                 120                 125                       

gcc atg gtc ggt gtc gca act gcg aaa act ccc tgc ggc ccc tat aca        432
Ala Met Val Gly Val Ala Thr Ala Lys Thr Pro Cys Gly Pro Tyr Thr           
    130                 135                 140                           

ttc aaa ggc agc ttc aaa cct ctc ggc gca gat tct cgc gat gaa ggg        480
Phe Lys Gly Ser Phe Lys Pro Leu Gly Ala Asp Ser Arg Asp Glu Gly           
145                 150                 155                 160           

ctg ttt cag gat gac gac tcc gca caa acc gcc tac ctt ctc tat gcc        528
Leu Phe Gln Asp Asp Asp Ser Ala Gln Thr Ala Tyr Leu Leu Tyr Ala           
                165                 170                 175               

tcc gat aac aac cag aac ttc aag ata tca agg ttg gat gac aac tat        576
Ser Asp Asn Asn Gln Asn Phe Lys Ile Ser Arg Leu Asp Asp Asn Tyr           
            180                 185                 190                   

tac aat gtg act gct cag gct agc gtt ttg acc ggt gcc aca ctt gaa        624
Tyr Asn Val Thr Ala Gln Ala Ser Val Leu Thr Gly Ala Thr Leu Glu           
        195                 200                 205                       

gca ccg ggc att gta aag cac agc gga aaa tac ttc cta atc gcc tcg        672
Ala Pro Gly Ile Val Lys His Ser Gly Lys Tyr Phe Leu Ile Ala Ser           
    210                 215                 220                           

cac acc agc gga tgg gct ccg aac cca aac aag ttc ttc tca gca tct        720
His Thr Ser Gly Trp Ala Pro Asn Pro Asn Lys Phe Phe Ser Ala Ser           
225                 230                 235                 240           

tcc ttg tcc ggc ccg tgg tcg tct caa caa gat atc acc acc gcg tcc        768
Ser Leu Ser Gly Pro Trp Ser Ser Gln Gln Asp Ile Thr Thr Ala Ser           
                245                 250                 255               

aca cgc acc tgg tac tcg caa aac gca ttc gac ttg cct ctc ggt aat        816
Thr Arg Thr Trp Tyr Ser Gln Asn Ala Phe Asp Leu Pro Leu Gly Asn           
            260                 265                 270                   

aat gcg atc tac atg gga gat agg tgg agg ccc agc tta ctt gga agc        864
Asn Ala Ile Tyr Met Gly Asp Arg Trp Arg Pro Ser Leu Leu Gly Ser           
        275                 280                 285                       

agc cgg tac atc tgg tat cct atc gat ttc tcc agc ggg tcg ccg caa        912
Ser Arg Tyr Ile Trp Tyr Pro Ile Asp Phe Ser Ser Gly Ser Pro Gln           
    290                 295                 300                           

ctc gtg cat gcc gat gtg tgg tcg gtg aac ccg tct gcc ggc acg tat        960
Leu Val His Ala Asp Val Trp Ser Val Asn Pro Ser Ala Gly Thr Tyr           
305                 310                 315                 320           

act gtt gct caa gga acc act tac gaa gct gag aag ggt aca ctc ggc       1008
Thr Val Ala Gln Gly Thr Thr Tyr Glu Ala Glu Lys Gly Thr Leu Gly           
                325                 330                 335               

ggg tct tcc aag ctc ttg tcc aat tcg ggg ttc tct ggg ggc agc gca       1056
Gly Ser Ser Lys Leu Leu Ser Asn Ser Gly Phe Ser Gly Gly Ser Ala           
            340                 345                 350                   

gta ggt tac ctc ggt cat ggt ggc act gtg acg atc aac aac gtt caa       1104
Val Gly Tyr Leu Gly His Gly Gly Thr Val Thr Ile Asn Asn Val Gln           
        355                 360                 365                       

ggc aat gga gga gcg cac tgg gtt gcg atc tat ttt gcc aat ggc gac       1152
Gly Asn Gly Gly Ala His Trp Val Ala Ile Tyr Phe Ala Asn Gly Asp           
    370                 375                 380                           

tcc aca tat agg aat gtc acc gtc agc gta aat gga ggc tcc tct gtt       1200
Ser Thr Tyr Arg Asn Val Thr Val Ser Val Asn Gly Gly Ser Ser Val           
385                 390                 395                 400           

ctc gtc gac cag cca gac tct gga gga gga ggc gta gtc att agt gta       1248
Leu Val Asp Gln Pro Asp Ser Gly Gly Gly Gly Val Val Ile Ser Val           
                405                 410                 415               

cct gtc aaa gtg aac ctg aat aat ggc gca aac tcg atc acc ttt ggc       1296
Pro Val Lys Val Asn Leu Asn Asn Gly Ala Asn Ser Ile Thr Phe Gly           
            420                 425                 430                   

tcc gga caa tcc aat tac gct gcc gac ctt gac aag att atc gtc tac       1344
Ser Gly Gln Ser Asn Tyr Ala Ala Asp Leu Asp Lys Ile Ile Val Tyr           
        435                 440                 445                       

tga                                                                   1347


<210>  10
<211>  448
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  10

Met Leu Trp Leu Arg Lys Val Ile Pro Val Phe Leu Ser Leu Val Val 
1               5                   10                  15      


Ala Ala Arg Ala Glu Thr Gln Ile Val Ser Gly Ala Ala Trp Thr Asp 
            20                  25                  30          


Thr Ser Gly Asn Val Ile Gln Ala His Gly Ala Gly Ile Leu Lys Val 
        35                  40                  45              


Gly Ser Thr Phe Tyr Trp Phe Gly Glu Asp Lys Thr Glu Asn Ser Ala 
    50                  55                  60                  


Leu Phe His Ala Val Ser Cys Tyr Thr Ser Thr Asp Leu Thr Asn Trp 
65                  70                  75                  80  


Thr Arg Gln Ser Asn Ala Leu Ser Pro Val Ala Asn Thr Met Ile Ser 
                85                  90                  95      


Ser Asn Asn Ile Val Glu Arg Pro Lys Val Leu Phe Asn Lys Lys Asn 
            100                 105                 110         


Gln Glu Tyr Val Met Trp Phe His Ser Asp Ser Ser Asn Tyr Gly Ala 
        115                 120                 125             


Ala Met Val Gly Val Ala Thr Ala Lys Thr Pro Cys Gly Pro Tyr Thr 
    130                 135                 140                 


Phe Lys Gly Ser Phe Lys Pro Leu Gly Ala Asp Ser Arg Asp Glu Gly 
145                 150                 155                 160 


Leu Phe Gln Asp Asp Asp Ser Ala Gln Thr Ala Tyr Leu Leu Tyr Ala 
                165                 170                 175     


Ser Asp Asn Asn Gln Asn Phe Lys Ile Ser Arg Leu Asp Asp Asn Tyr 
            180                 185                 190         


Tyr Asn Val Thr Ala Gln Ala Ser Val Leu Thr Gly Ala Thr Leu Glu 
        195                 200                 205             


Ala Pro Gly Ile Val Lys His Ser Gly Lys Tyr Phe Leu Ile Ala Ser 
    210                 215                 220                 


His Thr Ser Gly Trp Ala Pro Asn Pro Asn Lys Phe Phe Ser Ala Ser 
225                 230                 235                 240 


Ser Leu Ser Gly Pro Trp Ser Ser Gln Gln Asp Ile Thr Thr Ala Ser 
                245                 250                 255     


Thr Arg Thr Trp Tyr Ser Gln Asn Ala Phe Asp Leu Pro Leu Gly Asn 
            260                 265                 270         


Asn Ala Ile Tyr Met Gly Asp Arg Trp Arg Pro Ser Leu Leu Gly Ser 
        275                 280                 285             


Ser Arg Tyr Ile Trp Tyr Pro Ile Asp Phe Ser Ser Gly Ser Pro Gln 
    290                 295                 300                 


Leu Val His Ala Asp Val Trp Ser Val Asn Pro Ser Ala Gly Thr Tyr 
305                 310                 315                 320 


Thr Val Ala Gln Gly Thr Thr Tyr Glu Ala Glu Lys Gly Thr Leu Gly 
                325                 330                 335     


Gly Ser Ser Lys Leu Leu Ser Asn Ser Gly Phe Ser Gly Gly Ser Ala 
            340                 345                 350         


Val Gly Tyr Leu Gly His Gly Gly Thr Val Thr Ile Asn Asn Val Gln 
        355                 360                 365             


Gly Asn Gly Gly Ala His Trp Val Ala Ile Tyr Phe Ala Asn Gly Asp 
    370                 375                 380                 


Ser Thr Tyr Arg Asn Val Thr Val Ser Val Asn Gly Gly Ser Ser Val 
385                 390                 395                 400 


Leu Val Asp Gln Pro Asp Ser Gly Gly Gly Gly Val Val Ile Ser Val 
                405                 410                 415     


Pro Val Lys Val Asn Leu Asn Asn Gly Ala Asn Ser Ile Thr Phe Gly 
            420                 425                 430         


Ser Gly Gln Ser Asn Tyr Ala Ala Asp Leu Asp Lys Ile Ile Val Tyr 
        435                 440                 445             


<210>  11
<211>  1224
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic gene


<220>
<221>  CDS
<222>  (1)..(1224)

<400>  11
atg agg ttg cag aag gat ttc gat cga gtc atg ggc att atc ttg atg         48
Met Arg Leu Gln Lys Asp Phe Asp Arg Val Met Gly Ile Ile Leu Met           
1               5                   10                  15                

att ctc att ctc gca atc ccc tcc ttg cag atc tgt aac aag tcg tac         96
Ile Leu Ile Leu Ala Ile Pro Ser Leu Gln Ile Cys Asn Lys Ser Tyr           
            20                  25                  30                    

act gag ggc gac ccg gtc atc atc aac ccg gtc cct ggc ctc ccc gtc        144
Thr Glu Gly Asp Pro Val Ile Ile Asn Pro Val Pro Gly Leu Pro Val           
        35                  40                  45                        

gac ttc att cga ggt gtg gat gca tcg gaa gca ccc tgg atc atc gag        192
Asp Phe Ile Arg Gly Val Asp Ala Ser Glu Ala Pro Trp Ile Ile Glu           
    50                  55                  60                            

ttg ggt ggc aag tac tac gac gag aac gga gtc gag agg gat ctc ttg        240
Leu Gly Gly Lys Tyr Tyr Asp Glu Asn Gly Val Glu Arg Asp Leu Leu           
65                  70                  75                  80            

gac atc ttg aag gaa aac ggc gtg aac tgg atc agg ctc cga gtg tgg        288
Asp Ile Leu Lys Glu Asn Gly Val Asn Trp Ile Arg Leu Arg Val Trp           
                85                  90                  95                

aac gat cct tat gac gaa cag ggt agg cct tat gga gga ggc aac tgt        336
Asn Asp Pro Tyr Asp Glu Gln Gly Arg Pro Tyr Gly Gly Gly Asn Cys           
            100                 105                 110                   

gat ctc cct cgc atg acc gac ttc gca gca aag gcg aag gcg aaa ggc        384
Asp Leu Pro Arg Met Thr Asp Phe Ala Ala Lys Ala Lys Ala Lys Gly           
        115                 120                 125                       

ttc gga gtg ttg att gat ttc cac tat tcg gat tgg tgg gca gac ccc        432
Phe Gly Val Leu Ile Asp Phe His Tyr Ser Asp Trp Trp Ala Asp Pro           
    130                 135                 140                           

tcg aaa cag tcg aag ccc aag gcc tgg gcc aac ctc tcg tac ccc gag        480
Ser Lys Gln Ser Lys Pro Lys Ala Trp Ala Asn Leu Ser Tyr Pro Glu           
145                 150                 155                 160           

ctc gtg gaa gcg gtc tat aac tgg acc tac aac gcg ttg aag tac atg        528
Leu Val Glu Ala Val Tyr Asn Trp Thr Tyr Asn Ala Leu Lys Tyr Met           
                165                 170                 175               

gcc gag cac aac gca ttg ccc gat atg gtg cag atc gga aac gag att        576
Ala Glu His Asn Ala Leu Pro Asp Met Val Gln Ile Gly Asn Glu Ile           
            180                 185                 190                   

aac aac ggc ttc ctc tgg cct gat ggc tcg gca gcc aac tgg aca cag        624
Asn Asn Gly Phe Leu Trp Pro Asp Gly Ser Ala Ala Asn Trp Thr Gln           
        195                 200                 205                       

ttc gtc ggt ttg ttg aaa gca gcc att tcg gca gtg aaa gac gtg aac        672
Phe Val Gly Leu Leu Lys Ala Ala Ile Ser Ala Val Lys Asp Val Asn           
    210                 215                 220                           

ccc aac atc aag att gtc atc cat ctc gca gga gtc aag gcg gat ttc        720
Pro Asn Ile Lys Ile Val Ile His Leu Ala Gly Val Lys Ala Asp Phe           
225                 230                 235                 240           

tac atc aac ttc att gac agg ttg atc aac tcc gga gtc tcc ttc gac        768
Tyr Ile Asn Phe Ile Asp Arg Leu Ile Asn Ser Gly Val Ser Phe Asp           
                245                 250                 255               

gtc atc gca atc tcg ttc tat ccc tat tgg cat ggt acc atg gac gac        816
Val Ile Ala Ile Ser Phe Tyr Pro Tyr Trp His Gly Thr Met Asp Asp           
            260                 265                 270                   

ttc agg aac ttg gtg cgc act ctc gtg cag agg tac gat aag aag atc        864
Phe Arg Asn Leu Val Arg Thr Leu Val Gln Arg Tyr Asp Lys Lys Ile           
        275                 280                 285                       

ctc gtc gca gag acc gcg tac gcc tgg acc ctc gat gat tcc gat gga        912
Leu Val Ala Glu Thr Ala Tyr Ala Trp Thr Leu Asp Asp Ser Asp Gly           
    290                 295                 300                           

cac ccg aac att ttc ggt tcg agg gac ctc gag gtc aag gga ggt tac        960
His Pro Asn Ile Phe Gly Ser Arg Asp Leu Glu Val Lys Gly Gly Tyr           
305                 310                 315                 320           

aaa gcc tcg atc cag gga cag gcc tcg ttc atc cgg gac ctc atc gca       1008
Lys Ala Ser Ile Gln Gly Gln Ala Ser Phe Ile Arg Asp Leu Ile Ala           
                325                 330                 335               

gca ctc tac gag gag ggt aag gat aaa gca ctc ggc atc ttc tac tgg       1056
Ala Leu Tyr Glu Glu Gly Lys Asp Lys Ala Leu Gly Ile Phe Tyr Trp           
            340                 345                 350                   

ggt gca acc tgg att cct tat cct ggt gcc gga tgg aag act ggc gaa       1104
Gly Ala Thr Trp Ile Pro Tyr Pro Gly Ala Gly Trp Lys Thr Gly Glu           
        355                 360                 365                       

gga aac ccc tgg gag aac cag gca ttg ttc gac ttc aac ggt agg gcc       1152
Gly Asn Pro Trp Glu Asn Gln Ala Leu Phe Asp Phe Asn Gly Arg Ala           
    370                 375                 380                           

ttg cct tcc ctc aag gtc ttc agg ctc gtg tac gaa gcc cag ccc gtc       1200
Leu Pro Ser Leu Lys Val Phe Arg Leu Val Tyr Glu Ala Gln Pro Val           
385                 390                 395                 400           

gag atc aaa cct ttg gaa ctc taa                                       1224
Glu Ile Lys Pro Leu Glu Leu                                               
                405                                                       


<210>  12
<211>  407
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  12

Met Arg Leu Gln Lys Asp Phe Asp Arg Val Met Gly Ile Ile Leu Met 
1               5                   10                  15      


Ile Leu Ile Leu Ala Ile Pro Ser Leu Gln Ile Cys Asn Lys Ser Tyr 
            20                  25                  30          


Thr Glu Gly Asp Pro Val Ile Ile Asn Pro Val Pro Gly Leu Pro Val 
        35                  40                  45              


Asp Phe Ile Arg Gly Val Asp Ala Ser Glu Ala Pro Trp Ile Ile Glu 
    50                  55                  60                  


Leu Gly Gly Lys Tyr Tyr Asp Glu Asn Gly Val Glu Arg Asp Leu Leu 
65                  70                  75                  80  


Asp Ile Leu Lys Glu Asn Gly Val Asn Trp Ile Arg Leu Arg Val Trp 
                85                  90                  95      


Asn Asp Pro Tyr Asp Glu Gln Gly Arg Pro Tyr Gly Gly Gly Asn Cys 
            100                 105                 110         


Asp Leu Pro Arg Met Thr Asp Phe Ala Ala Lys Ala Lys Ala Lys Gly 
        115                 120                 125             


Phe Gly Val Leu Ile Asp Phe His Tyr Ser Asp Trp Trp Ala Asp Pro 
    130                 135                 140                 


Ser Lys Gln Ser Lys Pro Lys Ala Trp Ala Asn Leu Ser Tyr Pro Glu 
145                 150                 155                 160 


Leu Val Glu Ala Val Tyr Asn Trp Thr Tyr Asn Ala Leu Lys Tyr Met 
                165                 170                 175     


Ala Glu His Asn Ala Leu Pro Asp Met Val Gln Ile Gly Asn Glu Ile 
            180                 185                 190         


Asn Asn Gly Phe Leu Trp Pro Asp Gly Ser Ala Ala Asn Trp Thr Gln 
        195                 200                 205             


Phe Val Gly Leu Leu Lys Ala Ala Ile Ser Ala Val Lys Asp Val Asn 
    210                 215                 220                 


Pro Asn Ile Lys Ile Val Ile His Leu Ala Gly Val Lys Ala Asp Phe 
225                 230                 235                 240 


Tyr Ile Asn Phe Ile Asp Arg Leu Ile Asn Ser Gly Val Ser Phe Asp 
                245                 250                 255     


Val Ile Ala Ile Ser Phe Tyr Pro Tyr Trp His Gly Thr Met Asp Asp 
            260                 265                 270         


Phe Arg Asn Leu Val Arg Thr Leu Val Gln Arg Tyr Asp Lys Lys Ile 
        275                 280                 285             


Leu Val Ala Glu Thr Ala Tyr Ala Trp Thr Leu Asp Asp Ser Asp Gly 
    290                 295                 300                 


His Pro Asn Ile Phe Gly Ser Arg Asp Leu Glu Val Lys Gly Gly Tyr 
305                 310                 315                 320 


Lys Ala Ser Ile Gln Gly Gln Ala Ser Phe Ile Arg Asp Leu Ile Ala 
                325                 330                 335     


Ala Leu Tyr Glu Glu Gly Lys Asp Lys Ala Leu Gly Ile Phe Tyr Trp 
            340                 345                 350         


Gly Ala Thr Trp Ile Pro Tyr Pro Gly Ala Gly Trp Lys Thr Gly Glu 
        355                 360                 365             


Gly Asn Pro Trp Glu Asn Gln Ala Leu Phe Asp Phe Asn Gly Arg Ala 
    370                 375                 380                 


Leu Pro Ser Leu Lys Val Phe Arg Leu Val Tyr Glu Ala Gln Pro Val 
385                 390                 395                 400 


Glu Ile Lys Pro Leu Glu Leu 
                405         


<210>  13
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  13
cccttgtcga tgcgatgtat c                                                 21


<210>  14
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  14
atcctcaatt ccgtcggtcg a                                                 21


<210>  15
<211>  309
<212>  PRT
<213>  Bacillus sp.

<400>  15

Ala Asn Ser Gly Phe Tyr Val Ser Gly Thr Thr Leu Tyr Asp Ala Asn 
1               5                   10                  15      


Gly Asn Pro Phe Val Met Arg Gly Ile Asn His Gly His Ala Trp Tyr 
            20                  25                  30          


Lys Asp Gln Ala Thr Thr Ala Ile Glu Gly Ile Ala Asn Thr Gly Ala 
        35                  40                  45              


Asn Thr Val Arg Ile Val Leu Ser Asp Gly Gly Gln Trp Thr Lys Asp 
    50                  55                  60                  


Asp Ile His Thr Val Arg Asn Leu Ile Ser Leu Ala Glu Asp Asn His 
65                  70                  75                  80  


Leu Val Ala Val Leu Glu Val His Asp Ala Thr Gly Tyr Asp Ser Ile 
                85                  90                  95      


Ala Ser Leu Asn Arg Ala Val Asp Tyr Trp Ile Glu Met Arg Ser Ala 
            100                 105                 110         


Leu Ile Gly Lys Glu Asp Thr Val Ile Ile Asn Ile Ala Asn Glu Trp 
        115                 120                 125             


Phe Gly Ser Trp Glu Gly Asp Ala Trp Ala Asp Gly Tyr Lys Gln Ala 
    130                 135                 140                 


Ile Pro Arg Leu Arg Asn Ala Gly Leu Asn His Thr Leu Met Val Asp 
145                 150                 155                 160 


Ala Ala Gly Trp Gly Gln Phe Pro Gln Ser Ile His Asp Tyr Gly Arg 
                165                 170                 175     


Glu Val Phe Asn Ala Asp Pro Gln Arg Asn Thr Met Phe Ser Ile His 
            180                 185                 190         


Met Tyr Glu Tyr Ala Gly Gly Asn Ala Ser Gln Val Arg Thr Asn Ile 
        195                 200                 205             


Asp Arg Val Leu Asn Gln Asp Leu Ala Leu Val Ile Gly Glu Phe Gly 
    210                 215                 220                 


His Arg His Thr Asn Gly Asp Val Asp Glu Ala Thr Ile Met Ser Tyr 
225                 230                 235                 240 


Ser Glu Gln Arg Gly Val Gly Trp Leu Ala Trp Ser Trp Lys Gly Asn 
                245                 250                 255     


Gly Pro Glu Trp Glu Tyr Leu Asp Leu Ser Asn Asp Trp Ala Gly Asn 
            260                 265                 270         


Asn Leu Thr Ala Trp Gly Asn Thr Ile Val Asn Gly Pro Tyr Gly Leu 
        275                 280                 285             


Arg Glu Thr Ser Arg Leu Ser Thr Val Phe Thr Gly Gly Gly Ser Asp 
    290                 295                 300                 


Gly Gly Thr Ser Pro 
305                 


