                         SEQUENCE LISTING

<110>  Henkel AG & Co. KGaA
 
<120>  Detergent composition comprising protease variants

<130>  PT032016

<150>  EP13178385.4
<151>  2013-07-29

<160>  3     

<170>  PatentIn version 3.5

<210>  1
<211>  1263
<212>  DNA
<213>  bacillius sp.


<220>
<221>  CDS
<222>  (1)..(1263)

<220>
<221>  sig_peptide
<222>  (1)..(80)

<220>
<221>  mat_peptide
<222>  (331)..(1263)

<400>  1
atg  aag aaa ccg ttg ggg  aaa att gtc gca agc  acc gca cta ctc          45
Met  Lys Lys Pro Leu Gly  Lys Ile Val Ala Ser  Thr Ala Leu Leu            
-110                 -105                 -100                            

att tct gtt gct ttt agt tca tcg atc gca tcg gct gca ctt gca aaa         93
Ile Ser Val Ala Phe Ser Ser Ser Ile Ala Ser Ala Ala Leu Ala Lys           
-95                 -90                 -85                 -80           

gac aaa gtt gag gta aag gaa caa gat tca tat cgt gtg cta atc aaa        141
Asp Lys Val Glu Val Lys Glu Gln Asp Ser Tyr Arg Val Leu Ile Lys           
                -75                 -70                 -65               

gca cca act aca tca atc agt act ttt caa tca caa tac gat gtc cgt        189
Ala Pro Thr Thr Ser Ile Ser Thr Phe Gln Ser Gln Tyr Asp Val Arg           
            -60                 -55                 -50                   

tgg gat ttt ggc aaa gag gga ttt aca aca gat gtt gat gcc aaa cag        237
Trp Asp Phe Gly Lys Glu Gly Phe Thr Thr Asp Val Asp Ala Lys Gln           
        -45                 -40                 -35                       

ctc caa acg ctt caa agc aac aaa gac att caa att cag aag gta aat        285
Leu Gln Thr Leu Gln Ser Asn Lys Asp Ile Gln Ile Gln Lys Val Asn           
    -30                 -25                 -20                           

gaa atg aca gta gaa act gtt aca aca gaa aag gcg gaa gtg acg gcg        333
Glu Met Thr Val Glu Thr Val Thr Thr Glu Lys Ala Glu Val Thr Ala           
-15                 -10                 -5              -1  1             

gta cca agt aca caa acc cct tgg ggc ata aag tca att tat aat gat        381
Val Pro Ser Thr Gln Thr Pro Trp Gly Ile Lys Ser Ile Tyr Asn Asp           
            5                   10                  15                    

caa tca att aca aaa aca act gga ggc agc gga att aag gta gct gtt        429
Gln Ser Ile Thr Lys Thr Thr Gly Gly Ser Gly Ile Lys Val Ala Val           
        20                  25                  30                        

tta gat aca ggg gtt tat aca agc cat tta gat tta gct ggt tct gcc        477
Leu Asp Thr Gly Val Tyr Thr Ser His Leu Asp Leu Ala Gly Ser Ala           
    35                  40                  45                            

gag caa tgc aag gat ttt acc caa tct aat cct tta gta gat ggt tca        525
Glu Gln Cys Lys Asp Phe Thr Gln Ser Asn Pro Leu Val Asp Gly Ser           
50                  55                  60                  65            

tgc acc gat cgc caa ggg cat ggt aca cat gtt gcc gga act gta ttg        573
Cys Thr Asp Arg Gln Gly His Gly Thr His Val Ala Gly Thr Val Leu           
                70                  75                  80                

gcg cat gga ggc agt aat gga caa ggc gtt tac ggg gtg gct ccg caa        621
Ala His Gly Gly Ser Asn Gly Gln Gly Val Tyr Gly Val Ala Pro Gln           
            85                  90                  95                    

gcg aaa cta tgg gca tat aaa gta tta gga gat aac ggc agc gga tac        669
Ala Lys Leu Trp Ala Tyr Lys Val Leu Gly Asp Asn Gly Ser Gly Tyr           
        100                 105                 110                       

tct gat gat att gca gca gct atc aga cat gta gct gat gaa gct tca        717
Ser Asp Asp Ile Ala Ala Ala Ile Arg His Val Ala Asp Glu Ala Ser           
    115                 120                 125                           

cgt aca ggt tcc aaa gta gta att aat atg tcg cta ggt tca tct gcc        765
Arg Thr Gly Ser Lys Val Val Ile Asn Met Ser Leu Gly Ser Ser Ala           
130                 135                 140                 145           

aag gat tca ttg att gct agt gca gta gat tat gca tat gga aaa ggt        813
Lys Asp Ser Leu Ile Ala Ser Ala Val Asp Tyr Ala Tyr Gly Lys Gly           
                150                 155                 160               

gta tta atc gtt gct gcg gct ggt aat agt ggg tca ggc agc aat aca        861
Val Leu Ile Val Ala Ala Ala Gly Asn Ser Gly Ser Gly Ser Asn Thr           
            165                 170                 175                   

atc ggc ttt cct ggc ggg ctt gta aat gca gtg gca gta gcg gca ttg        909
Ile Gly Phe Pro Gly Gly Leu Val Asn Ala Val Ala Val Ala Ala Leu           
        180                 185                 190                       

gag aat gtt cag caa aat gga act tat cga gta gct gat ttc tca tct        957
Glu Asn Val Gln Gln Asn Gly Thr Tyr Arg Val Ala Asp Phe Ser Ser           
    195                 200                 205                           

aga ggg aat ccg gca act gct gga gat tat atc att caa gag cgt gat       1005
Arg Gly Asn Pro Ala Thr Ala Gly Asp Tyr Ile Ile Gln Glu Arg Asp           
210                 215                 220                 225           

att gaa gtt tca gct ccg gga gca agt gta gag tct aca tgg tac act       1053
Ile Glu Val Ser Ala Pro Gly Ala Ser Val Glu Ser Thr Trp Tyr Thr           
                230                 235                 240               

ggc ggt tat aat acg atc agc ggt aca tca atg gct aca cct cat gta       1101
Gly Gly Tyr Asn Thr Ile Ser Gly Thr Ser Met Ala Thr Pro His Val           
            245                 250                 255                   

gct ggg tta gct gct aaa atc tgg tca gcg aat act tca tta agt cat       1149
Ala Gly Leu Ala Ala Lys Ile Trp Ser Ala Asn Thr Ser Leu Ser His           
        260                 265                 270                       

agc caa ctg cgc aca gaa ttg caa aat cgc gct aaa gta tat gat att       1197
Ser Gln Leu Arg Thr Glu Leu Gln Asn Arg Ala Lys Val Tyr Asp Ile           
    275                 280                 285                           

aaa ggt ggt atc gga gcc gga aca ggt gac gat tat gca tca ggg ttc       1245
Lys Gly Gly Ile Gly Ala Gly Thr Gly Asp Asp Tyr Ala Ser Gly Phe           
290                 295                 300                 305           

gga tat cca aga gta aaa                                               1263
Gly Tyr Pro Arg Val Lys                                                   
                310                                                       


<210>  2
<211>  421
<212>  PRT
<213>  bacillius sp.

<400>  2

Met  Lys Lys Pro Leu Gly  Lys Ile Val Ala Ser  Thr Ala Leu Leu 
-110                 -105                 -100                 


Ile Ser Val Ala Phe Ser Ser Ser Ile Ala Ser Ala Ala Leu Ala Lys 
-95                 -90                 -85                 -80 


Asp Lys Val Glu Val Lys Glu Gln Asp Ser Tyr Arg Val Leu Ile Lys 
                -75                 -70                 -65     


Ala Pro Thr Thr Ser Ile Ser Thr Phe Gln Ser Gln Tyr Asp Val Arg 
            -60                 -55                 -50         


Trp Asp Phe Gly Lys Glu Gly Phe Thr Thr Asp Val Asp Ala Lys Gln 
        -45                 -40                 -35             


Leu Gln Thr Leu Gln Ser Asn Lys Asp Ile Gln Ile Gln Lys Val Asn 
    -30                 -25                 -20                 


Glu Met Thr Val Glu Thr Val Thr Thr Glu Lys Ala Glu Val Thr Ala 
-15                 -10                 -5              -1  1   


Val Pro Ser Thr Gln Thr Pro Trp Gly Ile Lys Ser Ile Tyr Asn Asp 
            5                   10                  15          


Gln Ser Ile Thr Lys Thr Thr Gly Gly Ser Gly Ile Lys Val Ala Val 
        20                  25                  30              


Leu Asp Thr Gly Val Tyr Thr Ser His Leu Asp Leu Ala Gly Ser Ala 
    35                  40                  45                  


Glu Gln Cys Lys Asp Phe Thr Gln Ser Asn Pro Leu Val Asp Gly Ser 
50                  55                  60                  65  


Cys Thr Asp Arg Gln Gly His Gly Thr His Val Ala Gly Thr Val Leu 
                70                  75                  80      


Ala His Gly Gly Ser Asn Gly Gln Gly Val Tyr Gly Val Ala Pro Gln 
            85                  90                  95          


Ala Lys Leu Trp Ala Tyr Lys Val Leu Gly Asp Asn Gly Ser Gly Tyr 
        100                 105                 110             


Ser Asp Asp Ile Ala Ala Ala Ile Arg His Val Ala Asp Glu Ala Ser 
    115                 120                 125                 


Arg Thr Gly Ser Lys Val Val Ile Asn Met Ser Leu Gly Ser Ser Ala 
130                 135                 140                 145 


Lys Asp Ser Leu Ile Ala Ser Ala Val Asp Tyr Ala Tyr Gly Lys Gly 
                150                 155                 160     


Val Leu Ile Val Ala Ala Ala Gly Asn Ser Gly Ser Gly Ser Asn Thr 
            165                 170                 175         


Ile Gly Phe Pro Gly Gly Leu Val Asn Ala Val Ala Val Ala Ala Leu 
        180                 185                 190             


Glu Asn Val Gln Gln Asn Gly Thr Tyr Arg Val Ala Asp Phe Ser Ser 
    195                 200                 205                 


Arg Gly Asn Pro Ala Thr Ala Gly Asp Tyr Ile Ile Gln Glu Arg Asp 
210                 215                 220                 225 


Ile Glu Val Ser Ala Pro Gly Ala Ser Val Glu Ser Thr Trp Tyr Thr 
                230                 235                 240     


Gly Gly Tyr Asn Thr Ile Ser Gly Thr Ser Met Ala Thr Pro His Val 
            245                 250                 255         


Ala Gly Leu Ala Ala Lys Ile Trp Ser Ala Asn Thr Ser Leu Ser His 
        260                 265                 270             


Ser Gln Leu Arg Thr Glu Leu Gln Asn Arg Ala Lys Val Tyr Asp Ile 
    275                 280                 285                 


Lys Gly Gly Ile Gly Ala Gly Thr Gly Asp Asp Tyr Ala Ser Gly Phe 
290                 295                 300                 305 


Gly Tyr Pro Arg Val Lys 
                310     


<210>  3
<211>  311
<212>  PRT
<213>  Bacillus sp.

<400>  3

Ala Val Pro Ser Thr Gln Thr Pro Trp Gly Ile Lys Ser Ile Tyr Asn 
1               5                   10                  15      


Asp Gln Ser Ile Thr Lys Thr Thr Gly Gly Ser Gly Ile Lys Val Ala 
            20                  25                  30          


Val Leu Asp Thr Gly Val Tyr Thr Ser His Leu Asp Leu Ala Gly Ser 
        35                  40                  45              


Ala Glu Gln Cys Lys Asp Phe Thr Gln Ser Asn Pro Leu Val Asp Gly 
    50                  55                  60                  


Ser Cys Thr Asp Arg Gln Gly His Gly Thr His Val Ala Gly Thr Val 
65                  70                  75                  80  


Leu Ala His Gly Gly Ser Asn Gly Gln Gly Val Tyr Gly Val Ala Pro 
                85                  90                  95      


Gln Ala Lys Leu Trp Ala Tyr Lys Val Leu Gly Asp Asn Gly Ser Gly 
            100                 105                 110         


Tyr Ser Asp Asp Ile Ala Ala Ala Ile Arg His Val Ala Asp Glu Ala 
        115                 120                 125             


Ser Arg Thr Gly Ser Lys Val Val Ile Asn Met Ser Leu Gly Ser Ser 
    130                 135                 140                 


Ala Lys Asp Ser Leu Ile Ala Ser Ala Val Asp Tyr Ala Tyr Gly Lys 
145                 150                 155                 160 


Gly Val Leu Ile Val Ala Ala Ala Gly Asn Ser Gly Ser Gly Ser Asn 
                165                 170                 175     


Thr Ile Gly Phe Pro Gly Gly Leu Val Asn Ala Val Ala Val Ala Ala 
            180                 185                 190         


Leu Glu Asn Val Gln Gln Asn Gly Thr Tyr Arg Val Ala Asp Phe Ser 
        195                 200                 205             


Ser Arg Gly Asn Pro Ala Thr Ala Gly Asp Tyr Ile Ile Gln Glu Arg 
    210                 215                 220                 


Asp Ile Glu Val Ser Ala Pro Gly Ala Ser Val Glu Ser Thr Trp Tyr 
225                 230                 235                 240 


Thr Gly Gly Tyr Asn Thr Ile Ser Gly Thr Ser Met Ala Thr Pro His 
                245                 250                 255     


Val Ala Gly Leu Ala Ala Lys Ile Trp Ser Ala Asn Thr Ser Leu Ser 
            260                 265                 270         


His Ser Gln Leu Arg Thr Glu Leu Gln Asn Arg Ala Lys Val Tyr Asp 
        275                 280                 285             


Ile Lys Gly Gly Ile Gly Ala Gly Thr Gly Asp Asp Tyr Ala Ser Gly 
    290                 295                 300                 


Phe Gly Tyr Pro Arg Val Lys 
305                 310     


