                         SEQUENCE LISTING

<110>  Novozymes A/S
 
<120>  Regulated PepC Expression

<130>  12683-WO-PCT

<160>  58    

<170>  PatentIn version 3.5

<210>  1
<211>  1485
<212>  DNA
<213>  Aspergillus oryzae


<220>
<221>  CDS
<222>  (1)..(1485)
<223>  PepC protease encoding sequence

<220>
<221>  sig_peptide
<222>  (1)..(48)

<220>
<221>  misc_feature
<222>  (49)..(345)
<223>  Pro-peptide

<220>
<221>  mat_peptide
<222>  (346)..(1485)

<400>  1
atg  aga ggc atc ctc ggc  ctt tcc ctg ctg cca  cta cta gca gcg          45
Met  Arg Gly Ile Leu Gly  Leu Ser Leu Leu Pro  Leu Leu Ala Ala            
-115                 -110                 -105                            

gcc  tcc ccc gtt gct gtt gac tcc atc cac aac gga gcg gct ccc att        93
Ala  Ser Pro Val Ala Val Asp Ser Ile His Asn Gly Ala Ala Pro Ile          
-100                 -95                 -90                 -85          

ctt tcg gcc tca aat gcc aaa gag gtt cca gac tct tac att gtc gtc        141
Leu Ser Ala Ser Asn Ala Lys Glu Val Pro Asp Ser Tyr Ile Val Val           
                -80                 -75                 -70               

ttc aag aag cat gtt tcc gct gaa acg gct gct gct cat cac acc tgg        189
Phe Lys Lys His Val Ser Ala Glu Thr Ala Ala Ala His His Thr Trp           
            -65                 -60                 -55                   

gtg cag gac atc cac gat tcg atg act gga cgc atc gac ctg aag aag        237
Val Gln Asp Ile His Asp Ser Met Thr Gly Arg Ile Asp Leu Lys Lys           
        -50                 -45                 -40                       

cgc tct ctt ttt ggt ttc agt gat gac ctt tac ctc ggt ctc aag aac        285
Arg Ser Leu Phe Gly Phe Ser Asp Asp Leu Tyr Leu Gly Leu Lys Asn           
    -35                 -30                 -25                           

acc ttc gat atc gcc ggg tcc cta gcg ggc tac tcc gga cat ttc cat        333
Thr Phe Asp Ile Ala Gly Ser Leu Ala Gly Tyr Ser Gly His Phe His           
-20                 -15                 -10                 -5            

gag gat gtg atc gag cag gtc cgg aga cat cct gat gtt gaa tac atc        381
Glu Asp Val Ile Glu Gln Val Arg Arg His Pro Asp Val Glu Tyr Ile           
            -1  1               5                   10                    

gag aaa gac acc gaa gtc cac acc atg gag gag aca acc gag aag aat        429
Glu Lys Asp Thr Glu Val His Thr Met Glu Glu Thr Thr Glu Lys Asn           
        15                  20                  25                        

gct ccc tgg ggc ttg gct cgt atc tct cac cgt gac agc ctc tcg ttc        477
Ala Pro Trp Gly Leu Ala Arg Ile Ser His Arg Asp Ser Leu Ser Phe           
    30                  35                  40                            

ggt acc ttt aac aag tac ctg tat gct tcg gaa ggc ggt gag ggt gtc        525
Gly Thr Phe Asn Lys Tyr Leu Tyr Ala Ser Glu Gly Gly Glu Gly Val           
45                  50                  55                  60            

gat gct tat act att gac act ggt atc aac att gag cat gtc gat ttc        573
Asp Ala Tyr Thr Ile Asp Thr Gly Ile Asn Ile Glu His Val Asp Phe           
                65                  70                  75                

gag gat cga gca cac tgg gga aag acc atc cct agc aat gat gag gat        621
Glu Asp Arg Ala His Trp Gly Lys Thr Ile Pro Ser Asn Asp Glu Asp           
            80                  85                  90                    

gcg gat ggc aac gga cac gga act cac tgc tcc gga acc att gct ggt        669
Ala Asp Gly Asn Gly His Gly Thr His Cys Ser Gly Thr Ile Ala Gly           
        95                  100                 105                       

aag aag tac ggt gtt gcc aag aag gcc aac atc tat gcc gtc aag gtc        717
Lys Lys Tyr Gly Val Ala Lys Lys Ala Asn Ile Tyr Ala Val Lys Val           
    110                 115                 120                           

ttg agg tcc agc ggt tct ggc act atg tcc gat gtc gtt ctg ggt gtc        765
Leu Arg Ser Ser Gly Ser Gly Thr Met Ser Asp Val Val Leu Gly Val           
125                 130                 135                 140           

gag tgg gcc gtc cag tcc cac ctc aag aag gct aag gac gcc aaa gat        813
Glu Trp Ala Val Gln Ser His Leu Lys Lys Ala Lys Asp Ala Lys Asp           
                145                 150                 155               

gcc aag gtc aag ggt ttc aag ggc agc gtt gcc aac atg agt ctt ggt        861
Ala Lys Val Lys Gly Phe Lys Gly Ser Val Ala Asn Met Ser Leu Gly           
            160                 165                 170                   

ggt gcc aag tcc agg acc ctt gag gct gct gtc aat gct ggt gtt gag        909
Gly Ala Lys Ser Arg Thr Leu Glu Ala Ala Val Asn Ala Gly Val Glu           
        175                 180                 185                       

gct ggt ctt cac ttc gcc gtt gct gct ggt aac gac aat gcc gat gcc        957
Ala Gly Leu His Phe Ala Val Ala Ala Gly Asn Asp Asn Ala Asp Ala           
    190                 195                 200                           

tgc aac tac tcc cct gct gcc gct gag aat gcc atc act gtc ggt gcc       1005
Cys Asn Tyr Ser Pro Ala Ala Ala Glu Asn Ala Ile Thr Val Gly Ala           
205                 210                 215                 220           

tcg acc ctt cag gat gag cgt gct tac ttc tcc aac tac gga aag tgc       1053
Ser Thr Leu Gln Asp Glu Arg Ala Tyr Phe Ser Asn Tyr Gly Lys Cys           
                225                 230                 235               

act gac atc ttt gcc ccg ggt ccc aac att ctt tcc acc tgg act ggc       1101
Thr Asp Ile Phe Ala Pro Gly Pro Asn Ile Leu Ser Thr Trp Thr Gly           
            240                 245                 250                   

agc aag cac gct gtc aac acc atc tct gga acc tct atg gct tct cct       1149
Ser Lys His Ala Val Asn Thr Ile Ser Gly Thr Ser Met Ala Ser Pro           
        255                 260                 265                       

cac att gct ggt ctg ctg gcc tac ttc gtt tct ctg cag cct gct cag       1197
His Ile Ala Gly Leu Leu Ala Tyr Phe Val Ser Leu Gln Pro Ala Gln           
    270                 275                 280                           

gac tct gct ttc gct gtc gat gag ctt act cct gcc aag ctc aag aag       1245
Asp Ser Ala Phe Ala Val Asp Glu Leu Thr Pro Ala Lys Leu Lys Lys           
285                 290                 295                 300           

gat atc atc tcc atc gcc acc cag ggt gcc ctt act gat atc cca tct       1293
Asp Ile Ile Ser Ile Ala Thr Gln Gly Ala Leu Thr Asp Ile Pro Ser           
                305                 310                 315               

gac acc ccc aac ctt ctc gcc tgg aac ggc ggt ggt gcc gac aac tac       1341
Asp Thr Pro Asn Leu Leu Ala Trp Asn Gly Gly Gly Ala Asp Asn Tyr           
            320                 325                 330                   

acc cag att gtc gcc aag ggt gga tac aag gcc ggc agt gac aac ctt       1389
Thr Gln Ile Val Ala Lys Gly Gly Tyr Lys Ala Gly Ser Asp Asn Leu           
        335                 340                 345                       

aag gac cgc ttt gac gga cta gtc aac aag gcc gag aag ttg ctc gct       1437
Lys Asp Arg Phe Asp Gly Leu Val Asn Lys Ala Glu Lys Leu Leu Ala           
    350                 355                 360                           

gag gag ctt gga gct att tac agt gag atc cag ggt gct gtt gtt gca       1485
Glu Glu Leu Gly Ala Ile Tyr Ser Glu Ile Gln Gly Ala Val Val Ala           
365                 370                 375                 380           


<210>  2
<211>  495
<212>  PRT
<213>  Aspergillus oryzae

<400>  2

Met  Arg Gly Ile Leu Gly  Leu Ser Leu Leu Pro  Leu Leu Ala Ala 
-115                 -110                 -105                 


Ala  Ser Pro Val Ala Val Asp Ser Ile His Asn Gly Ala Ala Pro Ile 
-100                 -95                 -90                 -85 


Leu Ser Ala Ser Asn Ala Lys Glu Val Pro Asp Ser Tyr Ile Val Val 
                -80                 -75                 -70     


Phe Lys Lys His Val Ser Ala Glu Thr Ala Ala Ala His His Thr Trp 
            -65                 -60                 -55         


Val Gln Asp Ile His Asp Ser Met Thr Gly Arg Ile Asp Leu Lys Lys 
        -50                 -45                 -40             


Arg Ser Leu Phe Gly Phe Ser Asp Asp Leu Tyr Leu Gly Leu Lys Asn 
    -35                 -30                 -25                 


Thr Phe Asp Ile Ala Gly Ser Leu Ala Gly Tyr Ser Gly His Phe His 
-20                 -15                 -10                 -5  


Glu Asp Val Ile Glu Gln Val Arg Arg His Pro Asp Val Glu Tyr Ile 
            -1  1               5                   10          


Glu Lys Asp Thr Glu Val His Thr Met Glu Glu Thr Thr Glu Lys Asn 
        15                  20                  25              


Ala Pro Trp Gly Leu Ala Arg Ile Ser His Arg Asp Ser Leu Ser Phe 
    30                  35                  40                  


Gly Thr Phe Asn Lys Tyr Leu Tyr Ala Ser Glu Gly Gly Glu Gly Val 
45                  50                  55                  60  


Asp Ala Tyr Thr Ile Asp Thr Gly Ile Asn Ile Glu His Val Asp Phe 
                65                  70                  75      


Glu Asp Arg Ala His Trp Gly Lys Thr Ile Pro Ser Asn Asp Glu Asp 
            80                  85                  90          


Ala Asp Gly Asn Gly His Gly Thr His Cys Ser Gly Thr Ile Ala Gly 
        95                  100                 105             


Lys Lys Tyr Gly Val Ala Lys Lys Ala Asn Ile Tyr Ala Val Lys Val 
    110                 115                 120                 


Leu Arg Ser Ser Gly Ser Gly Thr Met Ser Asp Val Val Leu Gly Val 
125                 130                 135                 140 


Glu Trp Ala Val Gln Ser His Leu Lys Lys Ala Lys Asp Ala Lys Asp 
                145                 150                 155     


Ala Lys Val Lys Gly Phe Lys Gly Ser Val Ala Asn Met Ser Leu Gly 
            160                 165                 170         


Gly Ala Lys Ser Arg Thr Leu Glu Ala Ala Val Asn Ala Gly Val Glu 
        175                 180                 185             


Ala Gly Leu His Phe Ala Val Ala Ala Gly Asn Asp Asn Ala Asp Ala 
    190                 195                 200                 


Cys Asn Tyr Ser Pro Ala Ala Ala Glu Asn Ala Ile Thr Val Gly Ala 
205                 210                 215                 220 


Ser Thr Leu Gln Asp Glu Arg Ala Tyr Phe Ser Asn Tyr Gly Lys Cys 
                225                 230                 235     


Thr Asp Ile Phe Ala Pro Gly Pro Asn Ile Leu Ser Thr Trp Thr Gly 
            240                 245                 250         


Ser Lys His Ala Val Asn Thr Ile Ser Gly Thr Ser Met Ala Ser Pro 
        255                 260                 265             


His Ile Ala Gly Leu Leu Ala Tyr Phe Val Ser Leu Gln Pro Ala Gln 
    270                 275                 280                 


Asp Ser Ala Phe Ala Val Asp Glu Leu Thr Pro Ala Lys Leu Lys Lys 
285                 290                 295                 300 


Asp Ile Ile Ser Ile Ala Thr Gln Gly Ala Leu Thr Asp Ile Pro Ser 
                305                 310                 315     


Asp Thr Pro Asn Leu Leu Ala Trp Asn Gly Gly Gly Ala Asp Asn Tyr 
            320                 325                 330         


Thr Gln Ile Val Ala Lys Gly Gly Tyr Lys Ala Gly Ser Asp Asn Leu 
        335                 340                 345             


Lys Asp Arg Phe Asp Gly Leu Val Asn Lys Ala Glu Lys Leu Leu Ala 
    350                 355                 360                 


Glu Glu Leu Gly Ala Ile Tyr Ser Glu Ile Gln Gly Ala Val Val Ala 
365                 370                 375                 380 


<210>  3
<211>  1599
<212>  DNA
<213>  Aspergillus niger


<220>
<221>  CDS
<222>  (1)..(1599)
<223>  PepC protease encoding sequence

<220>
<221>  sig_peptide
<222>  (1)..(48)

<220>
<221>  misc_feature
<222>  (49)..(345)
<223>  Pro-peptide

<220>
<221>  mat_peptide
<222>  (346)..(1599)

<400>  3
atg  aag ggc atc ctc ggc  ctt tcc ctc ctc ccg  ttg ctg acg gct          45
Met  Lys Gly Ile Leu Gly  Leu Ser Leu Leu Pro  Leu Leu Thr Ala            
-115                 -110                 -105                            

gcg  tcg ccc gtc ttc gtt gac tcc atc cat aat gaa gct gcc ccc atc        93
Ala  Ser Pro Val Phe Val Asp Ser Ile His Asn Glu Ala Ala Pro Ile          
-100                 -95                 -90                 -85          

ttg tct gct acc aac gcg aag gag gtt ccc gac tcc tac atc gtc gtt        141
Leu Ser Ala Thr Asn Ala Lys Glu Val Pro Asp Ser Tyr Ile Val Val           
                -80                 -75                 -70               

ttc aag aag cac gtc act tca gag ctg gct tcg gct cac cac agc tgg        189
Phe Lys Lys His Val Thr Ser Glu Leu Ala Ser Ala His His Ser Trp           
            -65                 -60                 -55                   

gtg cag gac atc cat gac tct cag agc gag cgg act gag ctg aag aag        237
Val Gln Asp Ile His Asp Ser Gln Ser Glu Arg Thr Glu Leu Lys Lys           
        -50                 -45                 -40                       

cgg tcg ctc ttc ggc ctt ggg gac gag gtc tat ctg ggt ctc aag aac        285
Arg Ser Leu Phe Gly Leu Gly Asp Glu Val Tyr Leu Gly Leu Lys Asn           
    -35                 -30                 -25                           

acc ttt gac att gct ggt tct ctg atc ggt tac tct ggt cac ttc cac        333
Thr Phe Asp Ile Ala Gly Ser Leu Ile Gly Tyr Ser Gly His Phe His           
-20                 -15                 -10                 -5            

gag gat gtc atc gag caa gtc cgc aga cac ccc gat gtc gat tac atc        381
Glu Asp Val Ile Glu Gln Val Arg Arg His Pro Asp Val Asp Tyr Ile           
            -1  1               5                   10                    

gag cgg gat tcc gaa gtt cac acc atg gaa ggg gcc acc gaa aag aac        429
Glu Arg Asp Ser Glu Val His Thr Met Glu Gly Ala Thr Glu Lys Asn           
        15                  20                  25                        

gcc cct tgg ggt ctg gct cgt atc tct cac cgt gat agc ctg acc ttc        477
Ala Pro Trp Gly Leu Ala Arg Ile Ser His Arg Asp Ser Leu Thr Phe           
    30                  35                  40                            

ggt aac ttc aac aag tac ctg tat gcc tcc gag ggg ggt gag ggc gtt        525
Gly Asn Phe Asn Lys Tyr Leu Tyr Ala Ser Glu Gly Gly Glu Gly Val           
45                  50                  55                  60            

gac gcc tac acc att gac acg ggt atc aac gtt gac cac gtt gac ttc        573
Asp Ala Tyr Thr Ile Asp Thr Gly Ile Asn Val Asp His Val Asp Phe           
                65                  70                  75                

gag ggc cgt gcc act tgg ggc aag aca atc cct acc aac gat gaa gat        621
Glu Gly Arg Ala Thr Trp Gly Lys Thr Ile Pro Thr Asn Asp Glu Asp           
            80                  85                  90                    

ctc gat ggc aat ggt cac gga act cac tgc tcc gga acc atg gct ggt        669
Leu Asp Gly Asn Gly His Gly Thr His Cys Ser Gly Thr Met Ala Gly           
        95                  100                 105                       

aag aag tac ggt gtt gcc aag aag gcc aac ctc tat gct gtc aag gtc        717
Lys Lys Tyr Gly Val Ala Lys Lys Ala Asn Leu Tyr Ala Val Lys Val           
    110                 115                 120                           

ctc cgg tcg agc ggc tct ggc acc atg tct gat gtc gtt tct ggt gtc        765
Leu Arg Ser Ser Gly Ser Gly Thr Met Ser Asp Val Val Ser Gly Val           
125                 130                 135                 140           

gag tat gcc gtc cag gct cat atc aag aag gcc aag gat gcc aag aac        813
Glu Tyr Ala Val Gln Ala His Ile Lys Lys Ala Lys Asp Ala Lys Asn           
                145                 150                 155               

ggc aag gtc aag gga ttc aag ggc agc gtt gcc aac atg agt ctc ggt        861
Gly Lys Val Lys Gly Phe Lys Gly Ser Val Ala Asn Met Ser Leu Gly           
            160                 165                 170                   

ggt ggc aag tct aag acc ctc gag gat gct gtt aac gct ggt gtt gag        909
Gly Gly Lys Ser Lys Thr Leu Glu Asp Ala Val Asn Ala Gly Val Glu           
        175                 180                 185                       

gct ggt ctt cac ttc gcc gtt gcc gcc ggt aat gac aat gct gat gct        957
Ala Gly Leu His Phe Ala Val Ala Ala Gly Asn Asp Asn Ala Asp Ala           
    190                 195                 200                           

tgc aac tac tct cct gct gct gcc gag aag gcc atc acc gtt ggt gcc       1005
Cys Asn Tyr Ser Pro Ala Ala Ala Glu Lys Ala Ile Thr Val Gly Ala           
205                 210                 215                 220           

tcg aca ctt gct gac gag cgt gcg tac ttc tcc aac tac gga gag tgc       1053
Ser Thr Leu Ala Asp Glu Arg Ala Tyr Phe Ser Asn Tyr Gly Glu Cys           
                225                 230                 235               

act gac atc ttc gct cct ggt ctc aac atc ctg tcc acc tgg att ggc       1101
Thr Asp Ile Phe Ala Pro Gly Leu Asn Ile Leu Ser Thr Trp Ile Gly           
            240                 245                 250                   

agc aac tac gcc acc aac atc atc tct ggc act tcc atg gcc tct cct       1149
Ser Asn Tyr Ala Thr Asn Ile Ile Ser Gly Thr Ser Met Ala Ser Pro           
        255                 260                 265                       

cac att gct ggc ctg ctg gcc tac ttt gtc tcc ctc cag ccc ccc tcg       1197
His Ile Ala Gly Leu Leu Ala Tyr Phe Val Ser Leu Gln Pro Pro Ser           
    270                 275                 280                           

gac tct gca ttc gct gtt gag gag ctt act cct gct aag ctg aag aag       1245
Asp Ser Ala Phe Ala Val Glu Glu Leu Thr Pro Ala Lys Leu Lys Lys           
285                 290                 295                 300           

gac atc atc gcc atc gcc acc gag ggc gct ctc act gac att ccc tcc       1293
Asp Ile Ile Ala Ile Ala Thr Glu Gly Ala Leu Thr Asp Ile Pro Ser           
                305                 310                 315               

aac acc ccc aac gta agt cat gcc gct gtt ggt att tat aag aga aac       1341
Asn Thr Pro Asn Val Ser His Ala Ala Val Gly Ile Tyr Lys Arg Asn           
            320                 325                 330                   

gag cta act cag aaa ttc agc tcc ttg cct gga acg gtg gtg gtt ccg       1389
Glu Leu Thr Gln Lys Phe Ser Ser Leu Pro Gly Thr Val Val Val Pro           
        335                 340                 345                       

aga act aca ccg aca tcg ttg gca gcg gtg gct aca agg tct cct ctg       1437
Arg Thr Thr Pro Thr Ser Leu Ala Ala Val Ala Thr Arg Ser Pro Leu           
    350                 355                 360                           

cca aga acc gca tcg agg acc gta ttg agg gtc tcg ttc aca agg ccg       1485
Pro Arg Thr Ala Ser Arg Thr Val Leu Arg Val Ser Phe Thr Arg Pro           
365                 370                 375                 380           

aag agc tgc tca ccg agg agc ttg gtg cca tct aca gcg aga tcc agg       1533
Lys Ser Cys Ser Pro Arg Ser Leu Val Pro Ser Thr Ala Arg Ser Arg           
                385                 390                 395               

atg ccg tcg tcg cat aga tca gaa ctc gtg ctt tcc aga ctt aga tcg       1581
Met Pro Ser Ser His Arg Ser Glu Leu Val Leu Ser Arg Leu Arg Ser           
            400                 405                 410                   

gaa gac ttg gtt ttt ttt                                               1599
Glu Asp Leu Val Phe Phe                                                   
        415                                                               


<210>  4
<211>  533
<212>  PRT
<213>  Aspergillus niger

<400>  4

Met  Lys Gly Ile Leu Gly  Leu Ser Leu Leu Pro  Leu Leu Thr Ala 
-115                 -110                 -105                 


Ala  Ser Pro Val Phe Val Asp Ser Ile His Asn Glu Ala Ala Pro Ile 
-100                 -95                 -90                 -85 


Leu Ser Ala Thr Asn Ala Lys Glu Val Pro Asp Ser Tyr Ile Val Val 
                -80                 -75                 -70     


Phe Lys Lys His Val Thr Ser Glu Leu Ala Ser Ala His His Ser Trp 
            -65                 -60                 -55         


Val Gln Asp Ile His Asp Ser Gln Ser Glu Arg Thr Glu Leu Lys Lys 
        -50                 -45                 -40             


Arg Ser Leu Phe Gly Leu Gly Asp Glu Val Tyr Leu Gly Leu Lys Asn 
    -35                 -30                 -25                 


Thr Phe Asp Ile Ala Gly Ser Leu Ile Gly Tyr Ser Gly His Phe His 
-20                 -15                 -10                 -5  


Glu Asp Val Ile Glu Gln Val Arg Arg His Pro Asp Val Asp Tyr Ile 
            -1  1               5                   10          


Glu Arg Asp Ser Glu Val His Thr Met Glu Gly Ala Thr Glu Lys Asn 
        15                  20                  25              


Ala Pro Trp Gly Leu Ala Arg Ile Ser His Arg Asp Ser Leu Thr Phe 
    30                  35                  40                  


Gly Asn Phe Asn Lys Tyr Leu Tyr Ala Ser Glu Gly Gly Glu Gly Val 
45                  50                  55                  60  


Asp Ala Tyr Thr Ile Asp Thr Gly Ile Asn Val Asp His Val Asp Phe 
                65                  70                  75      


Glu Gly Arg Ala Thr Trp Gly Lys Thr Ile Pro Thr Asn Asp Glu Asp 
            80                  85                  90          


Leu Asp Gly Asn Gly His Gly Thr His Cys Ser Gly Thr Met Ala Gly 
        95                  100                 105             


Lys Lys Tyr Gly Val Ala Lys Lys Ala Asn Leu Tyr Ala Val Lys Val 
    110                 115                 120                 


Leu Arg Ser Ser Gly Ser Gly Thr Met Ser Asp Val Val Ser Gly Val 
125                 130                 135                 140 


Glu Tyr Ala Val Gln Ala His Ile Lys Lys Ala Lys Asp Ala Lys Asn 
                145                 150                 155     


Gly Lys Val Lys Gly Phe Lys Gly Ser Val Ala Asn Met Ser Leu Gly 
            160                 165                 170         


Gly Gly Lys Ser Lys Thr Leu Glu Asp Ala Val Asn Ala Gly Val Glu 
        175                 180                 185             


Ala Gly Leu His Phe Ala Val Ala Ala Gly Asn Asp Asn Ala Asp Ala 
    190                 195                 200                 


Cys Asn Tyr Ser Pro Ala Ala Ala Glu Lys Ala Ile Thr Val Gly Ala 
205                 210                 215                 220 


Ser Thr Leu Ala Asp Glu Arg Ala Tyr Phe Ser Asn Tyr Gly Glu Cys 
                225                 230                 235     


Thr Asp Ile Phe Ala Pro Gly Leu Asn Ile Leu Ser Thr Trp Ile Gly 
            240                 245                 250         


Ser Asn Tyr Ala Thr Asn Ile Ile Ser Gly Thr Ser Met Ala Ser Pro 
        255                 260                 265             


His Ile Ala Gly Leu Leu Ala Tyr Phe Val Ser Leu Gln Pro Pro Ser 
    270                 275                 280                 


Asp Ser Ala Phe Ala Val Glu Glu Leu Thr Pro Ala Lys Leu Lys Lys 
285                 290                 295                 300 


Asp Ile Ile Ala Ile Ala Thr Glu Gly Ala Leu Thr Asp Ile Pro Ser 
                305                 310                 315     


Asn Thr Pro Asn Val Ser His Ala Ala Val Gly Ile Tyr Lys Arg Asn 
            320                 325                 330         


Glu Leu Thr Gln Lys Phe Ser Ser Leu Pro Gly Thr Val Val Val Pro 
        335                 340                 345             


Arg Thr Thr Pro Thr Ser Leu Ala Ala Val Ala Thr Arg Ser Pro Leu 
    350                 355                 360                 


Pro Arg Thr Ala Ser Arg Thr Val Leu Arg Val Ser Phe Thr Arg Pro 
365                 370                 375                 380 


Lys Ser Cys Ser Pro Arg Ser Leu Val Pro Ser Thr Ala Arg Ser Arg 
                385                 390                 395     


Met Pro Ser Ser His Arg Ser Glu Leu Val Leu Ser Arg Leu Arg Ser 
            400                 405                 410         


Glu Asp Leu Val Phe Phe 
        415             


<210>  5
<211>  19
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer oJaL113

<400>  5
gagctgctgg atttggctg                                                    19


<210>  6
<211>  19
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer oJaL114

<400>  6
ccaacagccg actcaggag                                                    19


<210>  7
<211>  19
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer oJaL228

<400>  7
accagcagca acggcgaag                                                    19


<210>  8
<211>  38
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer oJaL229

<400>  8
ggccttccct gccggtaaca tgagaggcat cctcggcc                               38


<210>  9
<211>  38
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer oJaL230

<400>  9
ggccgaggat gcctctcatg ttaccggcag ggaaggcc                               38


<210>  10
<211>  40
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer oJaL231

<400>  10
cgtccacgcg gggattatgc ggatgtggac gggttatcgg                             40


<210>  11
<211>  40
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer oJaL232

<400>  11
ccgataaccc gtccacatcc gcataatccc cgcgtggacg                             40


<210>  12
<211>  40
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer oJaL233

<400>  12
taatcactcc gaaaggtccc ccccgtcaag gagcttatcg                             40


<210>  13
<211>  40
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer oJaL234

<400>  13
cgataagctc cttgacgggg gggacctttc ggagtgatta                             40


<210>  14
<211>  18
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer oJaL235

<400>  14
gcacagccgt agtgggag                                                     18


<210>  15
<211>  27
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer  B2103F03

<400>  15
actagttaga atgctggacc agccccg                                           27


<210>  16
<211>  23
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer  B2103F04

<400>  16
aagcttattt gtctctgaca cac                                               23


<210>  17
<211>  46
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer P801

<400>  17
gaaacctgtc gtgccagtta attaagagag agttgaacct ggacgc                      46


<210>  18
<211>  46
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer P802

<400>  18
gcggccgctt ttttttgcga tcgcggtgac tgacacctgg cggtag                      46


<210>  19
<211>  45
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer P803

<400>  19
gcgatcgcaa aaaaaagcgg ccgcccccag ttgtgtatat agagg                       45


<210>  20
<211>  48
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer P804

<400>  20
gccgattcat taatgcaggg cgcgcctgaa tgtataagct agcttccg                    48


<210>  21
<211>  47
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer P805

<400>  21
tagcttatac attcaggcgc gccttcggta aatacactat cacacac                     47


<210>  22
<211>  51
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer P806

<400>  22
gattcattaa tgcagggcgc ggtttaaaca ttagtgatac cccactctaa g                51


<210>  23
<211>  39
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer P830

<400>  23
aaaaaaggcc ttcttggccc cacacaacat acgagccgg                              39


<210>  24
<211>  36
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer P831

<400>  24
aaaaaagctt ttatacattc aaatatgtat ccgctc                                 36


<210>  25
<211>  952
<212>  DNA
<213>  Humicola lanuginosa

<400>  25
gcggccgcac catgaagttc ttcaccacca tcctcagcac cgccagcctt gttgctgctc       60

tccccgccgc tgttgactcg aaccataccc cggccgctcc tgaacttgtt gcccggagtc      120

ctattcgtcg agaggtctcg caggatctgt ttaaccagtt caatctcttt gcacagtatt      180

ctgcagccgc atactgcgga aaaaacaatg atgccccagc tggtacaaac attacgtgcc      240

gcggacgggc ctgccccgag gtagagaagg cggatgcaac gtttctctac tcgtttgaag      300

actctggagt gggcgatgtc accggcttcc ttgctctcga caacacgaac aaattgatcg      360

tcctctcttt ccgtggctct cgttccatag agaactggat cgcgaatctt aacttcggct      420

tgaaagaaat aaatgacatt tgctccggct gcaggggaca tgacggcttc acttcgtcct      480

ggaggtctgt agccgatacg ttaaggcaga aggtggagga tgctgtgagg gagcatcccg      540

actatcgcgt ggtgtttacc ggacatagct tgggtggtgc attggcaact gttgccggag      600

cagacctgcg tggaaatggg tatgatatcg acgtgttttc atatggcgcc ccccgagtcg      660

gaaacagggc ttttgcagaa ttcctgaccg tacagaccgg cggaacactc taccgcatta      720

cccacaccaa tgatattgtc cctagactcc cgccgcgcga attcggttac agccattcta      780

gcccagaata ctggatcaaa tctggaaccc ttgtccccgt ccggcgacga gacatcgtga      840

agatagaagg catcgatgcc accggcggca ataaccagcc taacattccg gatatccctg      900

cgcacctatg gtacttcggg ttaattggga catgtcttta gtaagcgatc gc              952


<210>  26
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer P875

<400>  26
cagttgtcgc ttggtgcatc                                                   20


<210>  27
<211>  51
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer P882

<400>  27
cttagagtgg ggtatcacta ataagcttgt ttctgcatta atgaatcggc c                51


<210>  28
<211>  28
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer P881

<400>  28
aagcttatta gtgatacccc actctaag                                          28


<210>  29
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer P864

<400>  29
aggacttccc ctacggctcc g                                                 21


<210>  30
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer P200

<400>  30
ccaactccgc cgttgcatat c                                                 21


<210>  31
<211>  36
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer P920

<400>  31
ttttttttaa ttaattggcg gtgatattga tggcac                                 36


<210>  32
<211>  48
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer P921

<400>  32
aaaaaagctt gcatgcacta gtttatacat tcaaatatgt atccgctc                    48


<210>  33
<211>  43
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer P922

<400>  33
tagtgcatgc cctagggtcg acttaagcaa ggattttctt aac                         43


<210>  34
<211>  50
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer P923

<400>  34
cgaccctagg gcatgcacta gtctgtcaga ccaagtttac tcatatatac                  50


<210>  35
<211>  45
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer P924

<400>  35
aaaaaagctt actagtgcat gcgtttctgc attaatgaat cggcc                       45


<210>  36
<211>  42
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer MLxnoli20

<400>  36
caactggggg cggccgcacc atgaagctac tctctctgac cg                          42


<210>  37
<211>  34
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer MLxnoli21

<400>  37
gtcagtcacc gcgatcgctc agggggtgac gatg                                   34


<210>  38
<211>  690
<212>  DNA
<213>  Humicola insolens

<400>  38
atgaagttct tcaccacgat cctctcgact gcctcgttgg tcgcagcctt gcctgcggca       60

gtggattcca accacacacc ggcagcaccc gagctcgtgg cacgccagct cggagccatc      120

cagaacgact tggaatcggg ttcgcctgac gcgtgtcccg atgcaattct cattttcgca      180

cgaggatcga tggaacccgg taacatgggt atcactgtcg gacctgcgtt ggcaaacggt      240

ttgaaggagc atatccccaa catctggatt cagggagtgg gtggccctta cgacgcagcg      300

ctcgcaacca acttcttgcc tcgcggaacg tcgcaggcca acatcgacga gggaaaaagg      360

ctcttccacc tcgcccatca gaagtgtccc aacacaccgg tggtggcagg aggatactcc      420

cagggtgcag cgttgattgc cgcagccgtc tcggaattgt cgggagcagt gaaggagcag      480

gtcaagggag tcgtcttgtt cggatacacc cagaacctcc agaaccgagg aggcattccc      540

aactatcctc gcgagcgcac gaaggtgttc tgtaacgtgg gtgatgccgt gtgtacaggc      600

atcccgatca tcactcctgc ccacctctcg tataccatcc aggcgagggg tgaggcagca      660

cggttcctcg tcgaccgaat tagggcgtga                                       690


<210>  39
<211>  47
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer DAuP810

<400>  39
caactggggg cggccgcacc atgaagttct tcaccacgat cctctcg                     47


<210>  40
<211>  41
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer DAuP811

<400>  40
gtcagtcacc gcgatcgtca cgccctaatt cggtcgacga g                           41


<210>  41
<211>  1400
<212>  DNA
<213>  Aspergillus oryzae


<220>
<221>  promoter
<222>  (1)..(1400)
<223>  Aspergillus oryzae niaD promoter, PniaD.

<400>  41
gttaccggca gggaaggcca atgaaagtaa aattacgagg gagggagcat gaacaaggat       60

gctgagtatg aataagtcga atggtcagcc agtgcattaa ctccaaataa ggaggcaatc      120

caccacacta aaatactctt gcctatcgta tgatggcacg cagtacgtgt tacccatgcg      180

cgggcagtgg acattctatt aggtcacggc agtaactcct tgttaccata taacgcctcg      240

gagaaaggtc acaataagca atgctcctag gaacccacca gcgatttccg cggagtccca      300

aaatcagctc attctgggag gtgggacgct cgaaattagg gcaagccttc aggctggacg      360

gcgtcccacc gcttaaccaa gcgttgaggc aaataaatcg cgttgaccca cacaacactc      420

tcgaggctcc agccatttgt ccgctcaacc ttgcaggatt tctttttcgt catattaatt      480

ggttctttga agaatgatgg agacaatgcc gtgaagccat gtgcaacttc caattagaag      540

tgttgttgct tatcgtccga atgagcctgg ttcgcgtgga gaatgggcca gatgggagct      600

cacggctgtt agagcggagc tactactctg tacgtaccct tcaaaggaat tctcggtaag      660

tttgttagag ggatattgct cacgtttaat tggcactcca ggatccttta aatccaggca      720

aaaatcgctc gatctggctt tttttgccaa tcttggaagt ctaccgtata cttgtagtta      780

cacccttgag gatttaccac atgagaagaa caccaaccta actgcgtgat ttaaaacgcc      840

attgctatga tgcttgaaga gtgccggata tatcggcatc taacttagga tttgtcttac      900

gtacaatatt tatgacctgt ggtgaaacct gaggcaacaa gggggcgcga tttaccagac      960

tggcgttcac ataccaatac agtgcttaat tgtaggtctc atgggtggaa tgagatgacc     1020

ttccctttca tctattctta agaggaacag ggatggtacc cacaccatac cccgaagagc     1080

tcgtgatgta atagaccctt tcgtagtatg cgggttttta ttgagatgcc gatatgcaaa     1140

cttgtagtaa gactaataat aacaggtgca attaattgaa tttggggcct gttaagcttt     1200

tcgtccgacc accagtgacg tccgtccgat aagccgggaa cgatcgataa ggaggctatc     1260

ccgtcgatcc tgactaacct tttccagttg ttccttaact ttgcaccatc tccgcggagg     1320

aatccctgac ccaatcttat ggtgaggtgc tatcgtcctt atcaatccct gtcacgattg     1380

ccgataaccc gtccacatcc                                                 1400


<210>  42
<211>  1400
<212>  DNA
<213>  Aspergillus oryzae


<220>
<221>  promoter
<222>  (1)..(1400)
<223>  Aspergillus oryzae sorA promoter, PsorA.

<400>  42
ctccccgacc accaagttcc acgtcctgac cgcccagctg tctttccctc gctctgcccg       60

taattctgat ctccaggccg gattccaagt gctgtcgtct gacctcgaaa gcaccactat      120

ctactaccag ttctccaatg agtcaatcat cgtcgaccgc agcaacacca gtgctgccgc      180

gaagaccacc aatggaatcg tcagcaccaa tgagtctgga cgtctccggt tgttcgattt      240

gcagggcgat gcccaggaaa ttgagactct ggacctcacg gtcgttgtgg ataactctgt      300

cctcgagatc tatgccaatg gacgttttgc cctgagtact tgggctcggt atgtattctg      360

tgatggtcga caacaaggca ttggctaaca atctttcgca gttcctggta caagaactcg      420

actgacatca agttcttcca caacggtgcg ggtgaagtga cattcagcaa tgttactgtc      480

tccgagggcc tgtttgaagc ctggcctgag cgtgtctaat gtaagatatt gtgatggggt      540

agggaaaggg ttgcttgtat atatgagctc gatgtcatgg aaagtaaaat ctgtggtcga      600

gttaataatt ataaaaccta gttagttcag agtaatccat ctggttgtac atttcgtgaa      660

ttcttcaaaa cggcatacca ccgctggacc tctgggctgg cgatcgactc gggaagccga      720

gaacaggcga gcccggcaaa ccacctcgcc ggccgccgga gaccaggtta tcgatgtgaa      780

agtgtgccca aacgtcactt ccaaagtcta ttaaagaata cttcgtatcc atgaccaggt      840

ttgatacctg aagggaaact ccctggaaat aaactcatga tcacctcgtt cacctaatca      900

atatggttaa gccgagccgt ccctccacaa gcgtcatctt ccacgacggt gactatgacg      960

cgcacatgaa ctacgataaa aatcgctcat agatataacg attaccccat aaccggacgg     1020

gccgatcatt gcgtttattt tgtccctgtc ctgttgcctg aacgccggcg gaactgtatg     1080

ttgaccggaa ccgatcatcg aaaggtcagg ttccggcgga accgaacaca ctcaacccca     1140

tgggcagact actccaggtt tcgactccgg attatgacta tcattctaga ctagtgtcta     1200

gtgataatcc actcaatggt tcgcaattgt ggggctacag tgactatgta gtcttgggtt     1260

acagtttgtt ggagagtggg gatgtatatc ctggggtatt gtagattagt ataaaaccac     1320

acacggatcc ccacgtgtat ggtaggtatc cctaagtgtg ctcatattgt tcacttgtgt     1380

catactatta gtgcaccgaa                                                 1400


<210>  43
<211>  611
<212>  DNA
<213>  Aspergillus oryzae


<220>
<221>  promoter
<222>  (1)..(611)
<223>  Aspergillus oryzae sorB promoter, PsorB.

<400>  43
gacgcagtgt ccctgtatta aaagctgtac gataaaacgt ataatagaat ggaatgactt       60

gacatcatga tttggtggta ctttcgttgc gcctctggaa tctacgcgaa aatgaatgaa      120

attgacgagt actaaactgt ccaacactga tgttgacaaa caacatcgag caagatcggc      180

tcacttattc aataaggagg tagggtttca tatcgcgctg ttagcgctgt caaggcgagt      240

agtcgccaat ccgagtctct gggcgaaggc tttgctttgc ttcatcgggc cggaagggtc      300

cgtttctatg cgcagtgaaa ctgtgaaaga ccattaggca tacaccaacc gagtaaagga      360

tcagcgggag aaattaaagt cagatagaac agctcataag ttctccattt cccccacgac      420

cccgcaggtc ccaagctgga gatgtgagac cggaaccgaa gtgtggttgt tccgccggaa      480

ccgtgatgcc gatgaacata ctacttattt caataggccc ttcagcgatt ataaggatgc      540

agtttaaatc ttcctttttt catttctcca gattcacccc tgaatacctt gtttagagtt      600

tagggagcaa a                                                           611


<210>  44
<211>  28
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer HTJP-2

<400>  44
ccgcacgtgt caagcaaccc caatccgc                                          28


<210>  45
<211>  37
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer HTJP-3

<400>  45
cgcggatcca ccatgggtgt caatttcaaa gttcttg                                37


<210>  46
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer HTJP-24

<400>  46
ccgcaacaac aggttacaaa g                                                 21


<210>  47
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer HTJP-25

<400>  47
ccaggttacc catttcgatg                                                   20


<210>  48
<211>  44
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer HTJP-231

<400>  48
ctaactacta actaggttaa ttaactcccc gaccaccaag ttcc                        44


<210>  49
<211>  36
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer HTJP-232

<400>  49
aaaatacttt actagtgcat gcatgaggtc tttttg                                 36


<210>  50
<211>  41
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer HTJP-233

<400>  50
gcccttcatg gtggctagct tcggtgcact aatagtatga c                           41


<210>  51
<211>  39
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer HTJP-234

<400>  51
gtgcaccgaa gctagccacc atgaagggca tcctcggcc                              39


<210>  52
<211>  30
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer HTJP-235

<400>  52
ccttaattaa gacgcagtgt ccctgtatta                                        30


<210>  53
<211>  30
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer HTJP-236

<400>  53
ctagctagct ttgctcccta aactctaaac                                        30


<210>  54
<211>  35
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer HTJP-280

<400>  54
agttaattaa gctagcctcc ccgaccacca agttc                                  35


<210>  55
<211>  36
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer HTJP-281

<400>  55
gtaagactga gctagcgcat gcatgaggtc tttttg                                 36


<210>  56
<211>  31
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer HTJP-282

<400>  56
agttaattaa gctagcgacg cagtgtccct g                                      31


<210>  57
<211>  29
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer HTJP-341

<400>  57
ccttaattaa ggatgtggac gggttatcg                                         29


<210>  58
<211>  26
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer HTJP-342

<400>  58
ctagctagcg ttaccggcag ggaagg                                            26


