                         LISTADO DE SECUENCIAS

<110>  Universidad Miguel Hernndez de Elche
 
<120>  Vacuna recombinante frente al virus de la septicemia hemorrgica 
       vrica

<130>  ES2047.14

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  1327
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  ADNc del ectodominio de la glicoprotena G del VHSV

<400>  1
cagatcactc aacgacctcc ggtcgaaaac atctcgacgt accatgcaga ctgggacact       60

ccgctataca ctcatccctc caactgcagg gacgattcct ttgtcccgat tcgaccagct      120

caactcaggt gtcctcatga atttgaagac ataaacaagg gactggtttc cgtcccaacc      180

aggatcatcc atctcccgct atcggtcacc agcgtctccg cagtagcgag tggccactac      240

ctgcacagag tgacttatcg agtcacctgt tcgaccagct tctttggagg gcaaaccatc      300

gaaaagacca tcttggaggc gaaactgtct cgtcaggagg ccacagacga ggcaagcaag      360

gatcacgagt acccgttctt ccctgaaccc tcctgcatct ggatgaaaaa caatgtccat      420

aaggacataa ctcactatta caagacccca aaaacagtat cggtggatct ctacagcagg      480

aaatttctca accctgattt catagagggg gtttgcacaa cctcgccctg tcaaactcat      540

tggcagggag tctattgggt cggtgccaca cctaaagccc attgccccac gtcggaaaca      600

ctagaaggac acctgttcac caggacccat gatcacaggg tggtcaaggc aattgtggca      660

ggccatcatc cctgcgggac tcacaatggc atgcacagtg acattctgcg ggacagaatg      720

gatcaagact gacctggggg acctgatcca ggtgacagga ccggggggca cgaggaaact      780

gactccaaac aagtgtgtca ataccgatat ccagatgagg ggggcaacag acgacttttc      840

ttatctcaac catctcatca ccaacatggc tcaaagaacc gagtgcctag atgcccatag      900

tgatatcacc gcttctggga aagtatcctc atttctcctc tcaaagtttc gtcccagcca      960

ccctggacct ggcaaggcac actatcttct cgacggtcaa atcatgcgag gtgactgtga     1020

ctatgaggca gtagtcagca tcaactacaa tcgcgctcaa tacaagacgg tgaacaacac     1080

atggaaatca tggaaacggg tagacaacaa cacagacggg tacgatggga tgatatttgg     1140

ggacaaattg atcatcccgg acatcgaaaa gtatcagagt gtctatgaca gtggaatgct     1200

cgttcaaaga aaccttgtgg aagtccctca tctgagcatt gtgtttgtct ccaacacatc     1260

tgatctttcc actaatcaca tccacaccaa cctaatccct tcggattggt cattcaactg     1320

gagtctt                                                               1327


<210>  2
<211>  71
<212>  DNA
<213>  Limanda limanda

<400>  2
atgaagttca ctgccacctt cctcgtgatg ttcatcttcg tcctcatggt tgaacctgga       60

gagtgtggtg c                                                            71


<210>  3
<211>  2533
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  Promotor de la  beta-actina

<400>  3
ttcacacagg aaacagctat gaccatgatt acgccaagct tttagacctt cttacttttg       60

gggattatat aagtattttc tcaataaata tctcatatct tactgtggtt taactgctga      120

atctaaaatt ttaatacaaa agtagttata tttgttgtac attgtaaact ataacttaac      180

ttcagtttca gagaaactca tgtgctcaaa atgtaaaaaa agtttcctgt taaatatttt      240

gtaaatgtat tgaagacaaa ataagaaaaa aaaaaatata agccactaaa tcacactgtc      300

cttggtatca gcaagagatt ctgacataat cagctgtttt tgtttattac tgccattgaa      360

ggccatgtgc attagtccca agttacacat taaaaagtca catgtagctt accaacatca      420

gtgctgttca agcacagcct catctactat tcaaactgtg gcaccatcta aaatatgcca      480

gaattttttt atttaatgaa tttgaccctg aaatatgtat taatatcact cctgtgattt      540

ttttgtaatc agcttacaat tacaggaatg caagcctgat tcattacaag tttcactaca      600

ctttctctga caacatcacc tactgaactc agaccagcta gttgctcctt aagtatacaa      660

tcatgtcact aatcctcatt tcaatgaaaa atacccctat tgtacttggt acttggtaga      720

taaccacaga gcagtattat gccattattg tgaatacaat aagaggtaaa tgacctacag      780

agctgctgct gctgttgtgt tagattgtaa acacagcaca ggatcaagga ggtgtccatc      840

actatgacca atactagcac tttgcacagg ctctttgaaa ggctgaaaag agccttattg      900

gcgttatcac aacaaaatac gcaaatacgg aaaacaacgt attgaacttc gcaaacaaaa      960

aacagcgatt ttgatgaaaa tcgcttaggc cttgctcttc aaacaatcca gcttctcctt     1020

ctttcactct caagttgcaa gaagcaagtg tagcaatgtg cacgcgacag ccgggtgtgt     1080

gacgctggac caatcagagc gcagagctcc gaaagtttac cttttatggc tagagccggc     1140

atctgccgtc atataaaaga gcgcgcccag cgtctcagcc tcactttgag ctcctccaca     1200

cgcagctagt gcggaatatc atctgcctgt aacccattct ctaaagtcga caaacccccc     1260

caaacctaag gtgagttgat ctttaagctt tttacatttt cagctcgcat atatcaattc     1320

gaacgtttaa ttagaatgtt taaataaagc tagattaaat gattaggctc agttaccggt     1380

cttttttttc tcatttacgt gcgaactctg cttaaactct agttattctt tattaatatg     1440

tggttatttt tatatatgta tgttatcata actgtactgg ctatgtcagg tggtaatgac     1500

tgtaacgtta cgttactcgt tgtaggcacg acattgaatg ggccggtgtt gaaataagtc     1560

ttcaacccct tttaacctca aaatgtgctc tggttaacaa ggattttaac agctatcagt     1620

atgactgtgc ggttttaaag ccgttagtga ggcacgttgc acacttgatg gatggccgga     1680

atgggaagtt ctttatgcag gcagtgctgc agcagggtgt gacctacttt agctaacgtt     1740

agccggctaa ccagcattca tctgccggta acttgagtct aatattctct atgtgatatc     1800

gaagtgatca aagacacgtc tgttagctca ctttaaccaa ctgtagtgaa aaatagcgca     1860

gtgtgcagcc cttcaagtct ttcatttagg ctgattattc aatcatttta ttaactatta     1920

acgcgttact aaacgtaagg taacgtagtc agtttttaat aactggtgaa aagtactggt     1980

tgggtttaaa tggtgactta taattgtgtt ggagggggaa acctttttga taaaggctat     2040

ataatctcaa atgaatgggc tgaggatggt gttcacaggt gctttagtga agtccgctcg     2100

tgaagagtcg ctgaagtgac tgcagatctg tagcgcatgc gttttggcag acggccgttg     2160

aaattcggtt gagtaattga taccaggtga ggctagagga tgtagaaatt catttgtgta     2220

gaatttaggg agtggcctgg cgtgatgaat gtcgaaatcc gttccttttt actgaaccct     2280

atgtctctgc tgagtgccac accgccggca caaagcgtct caaaccattg ccttttatgg     2340

taataatgag aatgcagagg gacttccttt gtctggcaca tctgaggcgc gcattgtcac     2400

actagcaccc actagcggtc agactgcaga caaacaggaa gctgactcca catggtcaca     2460

tgctcactga agtgttgact tccctgacag ctgtgcactt tctaaaccgg ttttctcatt     2520

catttacagt tca                                                        2533


<210>  4
<211>  3941
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  cido nucleico recombinante

<400>  4
ttcacacagg aaacagctat gaccatgatt acgccaagct tttagacctt cttacttttg       60

gggattatat aagtattttc tcaataaata tctcatatct tactgtggtt taactgctga      120

atctaaaatt ttaatacaaa agtagttata tttgttgtac attgtaaact ataacttaac      180

ttcagtttca gagaaactca tgtgctcaaa atgtaaaaaa agtttcctgt taaatatttt      240

gtaaatgtat tgaagacaaa ataagaaaaa aaaaaatata agccactaaa tcacactgtc      300

cttggtatca gcaagagatt ctgacataat cagctgtttt tgtttattac tgccattgaa      360

ggccatgtgc attagtccca agttacacat taaaaagtca catgtagctt accaacatca      420

gtgctgttca agcacagcct catctactat tcaaactgtg gcaccatcta aaatatgcca      480

gaattttttt atttaatgaa tttgaccctg aaatatgtat taatatcact cctgtgattt      540

ttttgtaatc agcttacaat tacaggaatg caagcctgat tcattacaag tttcactaca      600

ctttctctga caacatcacc tactgaactc agaccagcta gttgctcctt aagtatacaa      660

tcatgtcact aatcctcatt tcaatgaaaa atacccctat tgtacttggt acttggtaga      720

taaccacaga gcagtattat gccattattg tgaatacaat aagaggtaaa tgacctacag      780

agctgctgct gctgttgtgt tagattgtaa acacagcaca ggatcaagga ggtgtccatc      840

actatgacca atactagcac tttgcacagg ctctttgaaa ggctgaaaag agccttattg      900

gcgttatcac aacaaaatac gcaaatacgg aaaacaacgt attgaacttc gcaaacaaaa      960

aacagcgatt ttgatgaaaa tcgcttaggc cttgctcttc aaacaatcca gcttctcctt     1020

ctttcactct caagttgcaa gaagcaagtg tagcaatgtg cacgcgacag ccgggtgtgt     1080

gacgctggac caatcagagc gcagagctcc gaaagtttac cttttatggc tagagccggc     1140

atctgccgtc atataaaaga gcgcgcccag cgtctcagcc tcactttgag ctcctccaca     1200

cgcagctagt gcggaatatc atctgcctgt aacccattct ctaaagtcga caaacccccc     1260

caaacctaag gtgagttgat ctttaagctt tttacatttt cagctcgcat atatcaattc     1320

gaacgtttaa ttagaatgtt taaataaagc tagattaaat gattaggctc agttaccggt     1380

cttttttttc tcatttacgt gcgaactctg cttaaactct agttattctt tattaatatg     1440

tggttatttt tatatatgta tgttatcata actgtactgg ctatgtcagg tggtaatgac     1500

tgtaacgtta cgttactcgt tgtaggcacg acattgaatg ggccggtgtt gaaataagtc     1560

ttcaacccct tttaacctca aaatgtgctc tggttaacaa ggattttaac agctatcagt     1620

atgactgtgc ggttttaaag ccgttagtga ggcacgttgc acacttgatg gatggccgga     1680

atgggaagtt ctttatgcag gcagtgctgc agcagggtgt gacctacttt agctaacgtt     1740

agccggctaa ccagcattca tctgccggta acttgagtct aatattctct atgtgatatc     1800

gaagtgatca aagacacgtc tgttagctca ctttaaccaa ctgtagtgaa aaatagcgca     1860

gtgtgcagcc cttcaagtct ttcatttagg ctgattattc aatcatttta ttaactatta     1920

acgcgttact aaacgtaagg taacgtagtc agtttttaat aactggtgaa aagtactggt     1980

tgggtttaaa tggtgactta taattgtgtt ggagggggaa acctttttga taaaggctat     2040

ataatctcaa atgaatgggc tgaggatggt gttcacaggt gctttagtga agtccgctcg     2100

tgaagagtcg ctgaagtgac tgcagatctg tagcgcatgc gttttggcag acggccgttg     2160

aaattcggtt gagtaattga taccaggtga ggctagagga tgtagaaatt catttgtgta     2220

gaatttaggg agtggcctgg cgtgatgaat gtcgaaatcc gttccttttt actgaaccct     2280

atgtctctgc tgagtgccac accgccggca caaagcgtct caaaccattg ccttttatgg     2340

taataatgag aatgcagagg gacttccttt gtctggcaca tctgaggcgc gcattgtcac     2400

actagcaccc actagcggtc agactgcaga caaacaggaa gctgactcca catggtcaca     2460

tgctcactga agtgttgact tccctgacag ctgtgcactt tctaaaccgg ttttctcatt     2520

catttacagt tcagccggta ccgagctcgg atccactagt aacggccgcc agtgtgctgg     2580

aattcgccct tatgaagttc actgccacct tcctcgtgat gttcatcttc gtcctcatgg     2640

ttgaacctgg agagtgtggt gcggccgctc agatcactca acgacctccg gtcgaaaaca     2700

tctcgacgta ccatgcagac tgggacactc cgctatacac tcatccctcc aactgcaggg     2760

acgattcctt tgtcccgatt cgaccagctc aactcaggtg tcctcatgaa tttgaagaca     2820

taaacaaggg actggtttcc gtcccaacca ggatcatcca tctcccgcta tcggtcacca     2880

gcgtctccgc agtagcgagt ggccactacc tgcacagagt gacttatcga gtcacctgtt     2940

cgaccagctt ctttggaggg caaaccatcg aaaagaccat cttggaggcg aaactgtctc     3000

gtcaggaggc cacagacgag gcaagcaagg atcacgagta cccgttcttc cctgaaccct     3060

cctgcatctg gatgaaaaac aatgtccata aggacataac tcactattac aagaccccaa     3120

aaacagtatc ggtggatctc tacagcagga aatttctcaa ccctgatttc atagaggggg     3180

tttgcacaac ctcgccctgt caaactcatt ggcagggagt ctattgggtc ggtgccacac     3240

ctaaagccca ttgccccacg tcggaaacac tagaaggaca cctgttcacc aggacccatg     3300

atcacagggt ggtcaaggca attgtggcag gccatcatcc ctggggactc acaatggcat     3360

gcacagtgac attctgcggg acagaatgga tcaagactga cctgggggac ctgatccagg     3420

tgacaggacc ggggggcacg aggaaactga ctccaaacaa gtgtgtcaat accgatatcc     3480

agatgagggg ggcaacagac gacttttctt atctcaacca tctcatcacc aacatggctc     3540

aaagaaccga gtgcctagat gcccatagtg atatcaccgc ttctgggaaa gtatcctcat     3600

ttctcctctc aaagtttcgt cccagccacc ctggacctgg caaggcacac tatcttctcg     3660

acggtcaaat catgcgaggt gactgtgact atgaggcagt agtcagcatc aactacaatc     3720

gcgctcaata caagacgatg aacaacacat ggaaatcatg gaaacgggta gacaacaaca     3780

cagacgggta cgatgggatg atatttgggg acaaattgat catcccggac atcgaaaagt     3840

atcagagtgt ctatgacagt ggaatgctcg ttcaaagaaa ccttgtggaa gtccctcatc     3900

tgagcattgt gtttgtctcc aacacatctg atctttcctg a                         3941


<210>  5
<211>  442
<212>  PRT
<213>  Secuencia artificial

<220>
<223>  Ectodominio de la gliocoprotena G del VHSV

<400>  5

Val Ile Leu Ile Ile Ile Ile Lys Ser Thr Thr Pro Gln Ile Thr Gln 
1               5                   10                  15      


Arg Pro Pro Val Glu Asn Ile Ser Thr Tyr His Ala Asp Trp Asp Thr 
            20                  25                  30          


Pro Leu Tyr Thr His Pro Ser Asn Cys Arg Asp Asp Ser Phe Val Pro 
        35                  40                  45              


Asn Arg Pro Ala Gln Leu Arg Cys Pro His Glu Phe Glu Asp Ile Asn 
    50                  55                  60                  


Lys Gly Leu Val Ser Val Pro Thr Arg Ile Ile His Leu Pro Leu Ser 
65                  70                  75                  80  


Val Thr Ser Val Ser Ala Val Ala Ser Gly His Tyr Leu His Arg Val 
                85                  90                  95      


Thr Tyr Arg Val Thr Cys Ser Thr Ser Phe Phe Gly Gly Gln Thr Ile 
            100                 105                 110         


Glu Lys Thr Ile Leu Glu Ala Lys Leu Ser Arg Gln Glu Ala Thr Asp 
        115                 120                 125             


Glu Ala Ser Lys Asp His Glu Tyr Pro Phe Phe Pro Glu Pro Ser Cys 
    130                 135                 140                 


Ile Trp Met Lys Asn Asn Val His Lys Asp Ile Thr His Tyr Tyr Lys 
145                 150                 155                 160 


Thr Pro Lys Thr Val Ser Val Asp Leu Tyr Ser Arg Lys Phe Leu Asn 
                165                 170                 175     


Pro Asp Phe Ile Glu Gly Val Cys Thr Thr Ser Pro Cys Gln Thr His 
            180                 185                 190         


Trp Gln Gly Val Tyr Trp Val Gly Ala Thr Pro Lys Ala His Cys Pro 
        195                 200                 205             


Thr Ser Glu Thr Leu Glu Gly His Leu Phe Thr Arg Thr His Asp His 
    210                 215                 220                 


Arg Val Val Lys Ala Ile Val Ala Gly His His Pro Trp Gly Leu Thr 
225                 230                 235                 240 


Met Ala Cys Thr Val Thr Phe Cys Gly Thr Glu Trp Ile Lys Thr Asp 
                245                 250                 255     


Leu Gly Asp Leu Ile Gln Val Thr Gly Pro Gly Gly Thr Arg Lys Leu 
            260                 265                 270         


Thr Pro Asn Lys Cys Val Asn Thr Asp Ile Gln Met Arg Gly Ala Thr 
        275                 280                 285             


Asp Asp Phe Ser Tyr Leu Asn His Leu Ile Thr Asn Met Ala Gln Arg 
    290                 295                 300                 


Thr Glu Cys Leu Asp Ala His Ser Asp Ile Thr Ala Ser Gly Lys Val 
305                 310                 315                 320 


Ser Ser Phe Leu Leu Ser Lys Phe Arg Pro Ser His Pro Gly Pro Gly 
                325                 330                 335     


Lys Ala His Tyr Leu Leu Asp Gly Gln Ile Met Arg Gly Asp Cys Asp 
            340                 345                 350         


Tyr Glu Ala Val Val Ser Ile Asn Tyr Asn Arg Ala Gln Tyr Lys Thr 
        355                 360                 365             


Met Asn Asn Thr Trp Lys Ser Trp Lys Arg Val Asp Asn Asn Thr Asp 
    370                 375                 380                 


Gly Tyr Asp Gly Met Ile Phe Gly Asp Lys Leu Ile Ile Pro Asp Ile 
385                 390                 395                 400 


Glu Lys Tyr Gln Ser Val Tyr Asp Ser Gly Met Leu Val Gln Arg Asn 
                405                 410                 415     


Leu Val Glu Val Pro His Leu Ser Ile Val Phe Val Ser Asn Thr Ser 
            420                 425                 430         


Asp Leu Ser Thr Asn His Ile His Thr Asn 
        435                 440         


<210>  6
<211>  22
<212>  PRT
<213>  Limanda limanda

<400>  6

Met Lys Phe Thr Ala Thr Phe Leu Val Met Phe Ile Phe Val Leu Met 
1               5                   10                  15      


Val Glu Pro Gly Glu Cys 
            20          


<210>  7
<211>  27
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  Cebador directo para deteccin de la glicoprotena G del VHSV

<400>  7
cgaccagctc aactcaggtg tcctcat                                           27


<210>  8
<211>  27
<212>  DNA
<213>  Secuencia artificial

<220>
<223>  Cebador inverso para deteccin de la glicoprotena G del VHSV

<400>  8
ggtgactcga taagtcactc tgtgcac                                           27


