                         SEQUENCE LISTING

<110>  CENTRO DE ESTUDIOS CIENTFICOS DE VALDIVIA
       San Martn, Alejandro
       Barros, Luis Felipe
       Ceballo, Sebastin
 
<120>  GENETICALLY ENCODED PROBE FOR QUANTIFICATION OF PYRUVATE 
       CONCENTRATION WITH HIGH SPATIAL AND TEMPORAL RESOLUTION, AND 
       METHODS USING THE SAME FOR QUANTIFYING PYRUVATE TRANSPORT, 
       CELLULAR PYRUVATE PRODUCTION AND CONSUMPTION, AND MITOCHONDRIAL 
       PYRUVATE CONSUMPTION IN INTACT CELLS

<130>  PXWO 391/2013

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  768
<212>  PRT
<213>  Escherichia coli

<400>  1

Met Val Ser Lys Gly Glu Glu Thr Thr Met Gly Val Ile Lys Pro Asp 
1               5                   10                  15      


Met Lys Ile Lys Leu Lys Met Glu Gly Asn Val Asn Gly His Ala Phe 
            20                  25                  30          


Val Ile Glu Gly Glu Gly Glu Gly Lys Pro Tyr Asp Gly Thr Asn Thr 
        35                  40                  45              


Ile Asn Leu Glu Val Lys Glu Gly Ala Pro Leu Pro Phe Ser Tyr Asp 
    50                  55                  60                  


Ile Leu Thr Thr Ala Phe Ala Tyr Gly Asn Arg Ala Phe Thr Lys Tyr 
65                  70                  75                  80  


Pro Asp Asp Ile Pro Asn Tyr Phe Lys Gln Ser Phe Pro Glu Gly Tyr 
                85                  90                  95      


Ser Trp Glu Arg Thr Met Thr Phe Glu Asp Lys Gly Ile Val Lys Val 
            100                 105                 110         


Lys Ser Asp Ile Ser Met Glu Glu Asp Ser Phe Ile Tyr Glu Ile His 
        115                 120                 125             


Leu Lys Gly Glu Asn Phe Pro Pro Asn Gly Pro Val Met Gln Lys Lys 
    130                 135                 140                 


Thr Thr Gly Trp Asp Ala Ser Thr Glu Arg Met Tyr Val Arg Asp Gly 
145                 150                 155                 160 


Val Leu Lys Gly Asp Val Lys His Lys Leu Leu Leu Glu Gly Gly Gly 
                165                 170                 175     


His His Arg Val Asp Phe Lys Thr Ile Tyr Arg Ala Lys Lys Ala Val 
            180                 185                 190         


Lys Leu Pro Asp Tyr His Phe Val Asp His Arg Ile Glu Ile Leu Asn 
        195                 200                 205             


His Asp Lys Asp Tyr Asn Lys Val Thr Val Tyr Glu Ser Ala Val Ala 
    210                 215                 220                 


Arg Asn Ser Thr Asp Gly Met Asp Glu Leu Tyr Lys Arg Ser Gly Thr 
225                 230                 235                 240 


Thr Ser Leu Tyr Lys Lys Ala Gly Ser Glu Phe Ala Leu Gly Thr Met 
                245                 250                 255     


Ala Tyr Ser Lys Ile Arg Gln Pro Lys Leu Ser Asp Val Ile Glu Gln 
            260                 265                 270         


Gln Leu Glu Phe Leu Ile Leu Glu Gly Thr Leu Arg Pro Gly Glu Lys 
        275                 280                 285             


Leu Pro Pro Glu Arg Glu Leu Ala Lys Gln Phe Asp Val Ser Arg Pro 
    290                 295                 300                 


Ser Leu Arg Glu Ala Ile Gln Arg Leu Glu Ala Lys Gly Leu Leu Leu 
305                 310                 315                 320 


Arg Arg Gln Gly Gly Gly Thr Phe Val Gln Ser Ser Leu Trp Gln Ser 
                325                 330                 335     


Phe Ser Asp Pro Leu Val Glu Leu Leu Ser Asp His Pro Glu Ser Gln 
            340                 345                 350         


Tyr Asp Leu Leu Glu Thr Arg His Ala Leu Glu Gly Ile Ala Ala Tyr 
        355                 360                 365             


Tyr Ala Ala Leu Arg Ser Thr Asp Glu Asp Lys Glu Arg Ile Arg Glu 
    370                 375                 380                 


Leu His His Ala Ile Glu Leu Ala Gln Gln Ser Gly Asp Leu Asp Ala 
385                 390                 395                 400 


Glu Ser Asn Ala Val Leu Gln Tyr Gln Ile Ala Val Thr Glu Ala Ala 
                405                 410                 415     


His Asn Val Val Leu Leu His Leu Leu Arg Cys Met Glu Pro Met Leu 
            420                 425                 430         


Ala Gln Asn Val Arg Gln Asn Phe Glu Leu Leu Tyr Ser Arg Arg Glu 
        435                 440                 445             


Met Leu Pro Leu Val Ser Ser His Arg Thr Arg Ile Phe Glu Ala Ile 
    450                 455                 460                 


Met Ala Gly Lys Pro Glu Glu Ala Arg Glu Ala Ser His Arg His Leu 
465                 470                 475                 480 


Ala Phe Ile Glu Glu Ile Leu Leu Asp Arg Ser Arg Glu Glu Ser Arg 
                485                 490                 495     


Arg Glu Arg Ser Leu Arg Arg Leu Glu Gln Arg Lys Asn Leu Lys Lys 
            500                 505                 510         


Gly Glu Phe Asp Pro Ala Phe Leu Tyr Lys Val Val Leu Lys Arg Ser 
        515                 520                 525             


Thr Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile 
    530                 535                 540                 


Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser 
545                 550                 555                 560 


Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Leu 
                565                 570                 575     


Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr 
            580                 585                 590         


Thr Leu Gly Tyr Gly Leu Gln Cys Phe Ala Arg Tyr Pro Asp His Met 
        595                 600                 605             


Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln 
    610                 615                 620                 


Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala 
625                 630                 635                 640 


Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys 
                645                 650                 655     


Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu 
            660                 665                 670         


Tyr Asn Tyr Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys 
        675                 680                 685             


Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly 
    690                 695                 700                 


Gly Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp 
705                 710                 715                 720 


Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser Ala 
                725                 730                 735     


Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu 
            740                 745                 750         


Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
        755                 760                 765             


<210>  2
<211>  753
<212>  PRT
<213>  Escherichia coli

<400>  2

Met Val Ser Lys Gly Glu Glu Thr Thr Met Gly Val Ile Lys Pro Asp 
1               5                   10                  15      


Met Lys Ile Lys Leu Lys Met Glu Gly Asn Val Asn Gly His Ala Phe 
            20                  25                  30          


Val Ile Glu Gly Glu Gly Glu Gly Lys Pro Tyr Asp Gly Thr Asn Thr 
        35                  40                  45              


Ile Asn Leu Glu Val Lys Glu Gly Ala Pro Leu Pro Phe Ser Tyr Asp 
    50                  55                  60                  


Ile Leu Thr Thr Ala Phe Ala Tyr Gly Asn Arg Ala Phe Thr Lys Tyr 
65                  70                  75                  80  


Pro Asp Asp Ile Pro Asn Tyr Phe Lys Gln Ser Phe Pro Glu Gly Tyr 
                85                  90                  95      


Ser Trp Glu Arg Thr Met Thr Phe Glu Asp Lys Gly Ile Val Lys Val 
            100                 105                 110         


Lys Ser Asp Ile Ser Met Glu Glu Asp Ser Phe Ile Tyr Glu Ile His 
        115                 120                 125             


Leu Lys Gly Glu Asn Phe Pro Pro Asn Gly Pro Val Met Gln Lys Lys 
    130                 135                 140                 


Thr Thr Gly Trp Asp Ala Ser Thr Glu Arg Met Tyr Val Arg Asp Gly 
145                 150                 155                 160 


Val Leu Lys Gly Asp Val Lys His Lys Leu Leu Leu Glu Gly Gly Gly 
                165                 170                 175     


His His Arg Val Asp Phe Lys Thr Ile Tyr Arg Ala Lys Lys Ala Val 
            180                 185                 190         


Lys Leu Pro Asp Tyr His Phe Val Asp His Arg Ile Glu Ile Leu Asn 
        195                 200                 205             


His Asp Lys Asp Tyr Asn Lys Val Thr Val Tyr Glu Ser Ala Val Ala 
    210                 215                 220                 


Arg Asn Ser Thr Asp Gly Met Asp Glu Leu Tyr Lys Arg Ser Gly Thr 
225                 230                 235                 240 


Met Ala Tyr Ser Lys Ile Arg Gln Pro Lys Leu Ser Asp Val Ile Glu 
                245                 250                 255     


Gln Gln Leu Glu Phe Leu Ile Leu Glu Gly Thr Leu Arg Pro Gly Glu 
            260                 265                 270         


Lys Leu Pro Pro Glu Arg Glu Leu Ala Lys Gln Phe Asp Val Ser Arg 
        275                 280                 285             


Pro Ser Leu Arg Glu Ala Ile Gln Arg Leu Glu Ala Lys Gly Leu Leu 
    290                 295                 300                 


Leu Arg Arg Gln Gly Gly Gly Thr Phe Val Gln Ser Ser Leu Trp Gln 
305                 310                 315                 320 


Ser Phe Ser Asp Pro Leu Val Glu Leu Leu Ser Asp His Pro Glu Ser 
                325                 330                 335     


Gln Tyr Asp Leu Leu Glu Thr Arg His Ala Leu Glu Gly Ile Ala Ala 
            340                 345                 350         


Tyr Tyr Ala Ala Leu Arg Ser Thr Asp Glu Asp Lys Glu Arg Ile Arg 
        355                 360                 365             


Glu Leu His His Ala Ile Glu Leu Ala Gln Gln Ser Gly Asp Leu Asp 
    370                 375                 380                 


Ala Glu Ser Asn Ala Val Leu Gln Tyr Gln Ile Ala Val Thr Glu Ala 
385                 390                 395                 400 


Ala His Asn Val Val Leu Leu His Leu Leu Arg Cys Met Glu Pro Met 
                405                 410                 415     


Leu Ala Gln Asn Val Arg Gln Asn Phe Glu Leu Leu Tyr Ser Arg Arg 
            420                 425                 430         


Glu Met Leu Pro Leu Val Ser Ser His Arg Thr Arg Ile Phe Glu Ala 
        435                 440                 445             


Ile Met Ala Gly Lys Pro Glu Glu Ala Arg Glu Ala Ser His Arg His 
    450                 455                 460                 


Leu Ala Phe Ile Glu Glu Ile Leu Leu Asp Arg Ser Arg Glu Glu Ser 
465                 470                 475                 480 


Arg Arg Glu Arg Ser Leu Arg Arg Leu Glu Gln Arg Lys Asn Leu Lys 
                485                 490                 495     


Lys Gly Glu Phe Asp Pro Ala Phe Leu Tyr Lys Val Val Leu Lys Arg 
            500                 505                 510         


Ser Thr Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro 
        515                 520                 525             


Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val 
    530                 535                 540                 


Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys 
545                 550                 555                 560 


Leu Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val 
                565                 570                 575     


Thr Thr Leu Gly Tyr Gly Leu Gln Cys Phe Ala Arg Tyr Pro Asp His 
            580                 585                 590         


Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val 
        595                 600                 605             


Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg 
    610                 615                 620                 


Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu 
625                 630                 635                 640 


Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu 
                645                 650                 655     


Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln 
            660                 665                 670         


Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu Asp 
        675                 680                 685             


Gly Gly Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly 
    690                 695                 700                 


Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser 
705                 710                 715                 720 


Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu 
                725                 730                 735     


Glu Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr 
            740                 745                 750         


Lys 
    


<210>  3
<211>  753
<212>  PRT
<213>  Escherichia coli

<400>  3

Met Val Ser Lys Gly Glu Glu Thr Thr Met Gly Val Ile Lys Pro Asp 
1               5                   10                  15      


Met Lys Ile Lys Leu Lys Met Glu Gly Asn Val Asn Gly His Ala Phe 
            20                  25                  30          


Val Ile Glu Gly Glu Gly Glu Gly Lys Pro Tyr Asp Gly Thr Asn Thr 
        35                  40                  45              


Ile Asn Leu Glu Val Lys Glu Gly Ala Pro Leu Pro Phe Ser Tyr Asp 
    50                  55                  60                  


Ile Leu Thr Thr Ala Phe Ala Tyr Gly Asn Arg Ala Phe Thr Lys Tyr 
65                  70                  75                  80  


Pro Asp Asp Ile Pro Asn Tyr Phe Lys Gln Ser Phe Pro Glu Gly Tyr 
                85                  90                  95      


Ser Trp Glu Arg Thr Met Thr Phe Glu Asp Lys Gly Ile Val Lys Val 
            100                 105                 110         


Lys Ser Asp Ile Ser Met Glu Glu Asp Ser Phe Ile Tyr Glu Ile His 
        115                 120                 125             


Leu Lys Gly Glu Asn Phe Pro Pro Asn Gly Pro Val Met Gln Lys Lys 
    130                 135                 140                 


Thr Thr Gly Trp Asp Ala Ser Thr Glu Arg Met Tyr Val Arg Asp Gly 
145                 150                 155                 160 


Val Leu Lys Gly Asp Val Lys His Lys Leu Leu Leu Glu Gly Gly Gly 
                165                 170                 175     


His His Arg Val Asp Phe Lys Thr Ile Tyr Arg Ala Lys Lys Ala Val 
            180                 185                 190         


Lys Leu Pro Asp Tyr His Phe Val Asp His Arg Ile Glu Ile Leu Asn 
        195                 200                 205             


His Asp Lys Asp Tyr Asn Lys Val Thr Val Tyr Glu Ser Ala Val Ala 
    210                 215                 220                 


Arg Asn Ser Thr Asp Gly Met Asp Glu Leu Tyr Lys Arg Ser Gly Thr 
225                 230                 235                 240 


Thr Ser Leu Tyr Lys Lys Ala Gly Ser Glu Phe Ala Leu Gly Thr Met 
                245                 250                 255     


Ala Tyr Ser Lys Ile Arg Gln Pro Lys Leu Ser Asp Val Ile Glu Gln 
            260                 265                 270         


Gln Leu Glu Phe Leu Ile Leu Glu Gly Thr Leu Arg Pro Gly Glu Lys 
        275                 280                 285             


Leu Pro Pro Glu Arg Glu Leu Ala Lys Gln Phe Asp Val Ser Arg Pro 
    290                 295                 300                 


Ser Leu Arg Glu Ala Ile Gln Arg Leu Glu Ala Lys Gly Leu Leu Leu 
305                 310                 315                 320 


Arg Arg Gln Gly Gly Gly Thr Phe Val Gln Ser Ser Leu Trp Gln Ser 
                325                 330                 335     


Phe Ser Asp Pro Leu Val Glu Leu Leu Ser Asp His Pro Glu Ser Gln 
            340                 345                 350         


Tyr Asp Leu Leu Glu Thr Arg His Ala Leu Glu Gly Ile Ala Ala Tyr 
        355                 360                 365             


Tyr Ala Ala Leu Arg Ser Thr Asp Glu Asp Lys Glu Arg Ile Arg Glu 
    370                 375                 380                 


Leu His His Ala Ile Glu Leu Ala Gln Gln Ser Gly Asp Leu Asp Ala 
385                 390                 395                 400 


Glu Ser Asn Ala Val Leu Gln Tyr Gln Ile Ala Val Thr Glu Ala Ala 
                405                 410                 415     


His Asn Val Val Leu Leu His Leu Leu Arg Cys Met Glu Pro Met Leu 
            420                 425                 430         


Ala Gln Asn Val Arg Gln Asn Phe Glu Leu Leu Tyr Ser Arg Arg Glu 
        435                 440                 445             


Met Leu Pro Leu Val Ser Ser His Arg Thr Arg Ile Phe Glu Ala Ile 
    450                 455                 460                 


Met Ala Gly Lys Pro Glu Glu Ala Arg Glu Ala Ser His Arg His Leu 
465                 470                 475                 480 


Ala Phe Ile Glu Glu Ile Leu Leu Asp Arg Ser Arg Glu Glu Ser Arg 
                485                 490                 495     


Arg Glu Arg Ser Leu Arg Arg Leu Glu Gln Arg Lys Asn Leu Lys Arg 
            500                 505                 510         


Ser Thr Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro 
        515                 520                 525             


Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val 
    530                 535                 540                 


Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys 
545                 550                 555                 560 


Leu Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val 
                565                 570                 575     


Thr Thr Leu Gly Tyr Gly Leu Gln Cys Phe Ala Arg Tyr Pro Asp His 
            580                 585                 590         


Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val 
        595                 600                 605             


Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg 
    610                 615                 620                 


Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu 
625                 630                 635                 640 


Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu 
                645                 650                 655     


Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln 
            660                 665                 670         


Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu Asp 
        675                 680                 685             


Gly Gly Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly 
    690                 695                 700                 


Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln Ser 
705                 710                 715                 720 


Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu 
                725                 730                 735     


Glu Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr 
            740                 745                 750         


Lys 
    


<210>  4
<211>  738
<212>  PRT
<213>  Escherichia coli

<400>  4

Met Val Ser Lys Gly Glu Glu Thr Thr Met Gly Val Ile Lys Pro Asp 
1               5                   10                  15      


Met Lys Ile Lys Leu Lys Met Glu Gly Asn Val Asn Gly His Ala Phe 
            20                  25                  30          


Val Ile Glu Gly Glu Gly Glu Gly Lys Pro Tyr Asp Gly Thr Asn Thr 
        35                  40                  45              


Ile Asn Leu Glu Val Lys Glu Gly Ala Pro Leu Pro Phe Ser Tyr Asp 
    50                  55                  60                  


Ile Leu Thr Thr Ala Phe Ala Tyr Gly Asn Arg Ala Phe Thr Lys Tyr 
65                  70                  75                  80  


Pro Asp Asp Ile Pro Asn Tyr Phe Lys Gln Ser Phe Pro Glu Gly Tyr 
                85                  90                  95      


Ser Trp Glu Arg Thr Met Thr Phe Glu Asp Lys Gly Ile Val Lys Val 
            100                 105                 110         


Lys Ser Asp Ile Ser Met Glu Glu Asp Ser Phe Ile Tyr Glu Ile His 
        115                 120                 125             


Leu Lys Gly Glu Asn Phe Pro Pro Asn Gly Pro Val Met Gln Lys Lys 
    130                 135                 140                 


Thr Thr Gly Trp Asp Ala Ser Thr Glu Arg Met Tyr Val Arg Asp Gly 
145                 150                 155                 160 


Val Leu Lys Gly Asp Val Lys His Lys Leu Leu Leu Glu Gly Gly Gly 
                165                 170                 175     


His His Arg Val Asp Phe Lys Thr Ile Tyr Arg Ala Lys Lys Ala Val 
            180                 185                 190         


Lys Leu Pro Asp Tyr His Phe Val Asp His Arg Ile Glu Ile Leu Asn 
        195                 200                 205             


His Asp Lys Asp Tyr Asn Lys Val Thr Val Tyr Glu Ser Ala Val Ala 
    210                 215                 220                 


Arg Asn Ser Thr Asp Gly Met Asp Glu Leu Tyr Lys Arg Ser Gly Thr 
225                 230                 235                 240 


Met Ala Tyr Ser Lys Ile Arg Gln Pro Lys Leu Ser Asp Val Ile Glu 
                245                 250                 255     


Gln Gln Leu Glu Phe Leu Ile Leu Glu Gly Thr Leu Arg Pro Gly Glu 
            260                 265                 270         


Lys Leu Pro Pro Glu Arg Glu Leu Ala Lys Gln Phe Asp Val Ser Arg 
        275                 280                 285             


Pro Ser Leu Arg Glu Ala Ile Gln Arg Leu Glu Ala Lys Gly Leu Leu 
    290                 295                 300                 


Leu Arg Arg Gln Gly Gly Gly Thr Phe Val Gln Ser Ser Leu Trp Gln 
305                 310                 315                 320 


Ser Phe Ser Asp Pro Leu Val Glu Leu Leu Ser Asp His Pro Glu Ser 
                325                 330                 335     


Gln Tyr Asp Leu Leu Glu Thr Arg His Ala Leu Glu Gly Ile Ala Ala 
            340                 345                 350         


Tyr Tyr Ala Ala Leu Arg Ser Thr Asp Glu Asp Lys Glu Arg Ile Arg 
        355                 360                 365             


Glu Leu His His Ala Ile Glu Leu Ala Gln Gln Ser Gly Asp Leu Asp 
    370                 375                 380                 


Ala Glu Ser Asn Ala Val Leu Gln Tyr Gln Ile Ala Val Thr Glu Ala 
385                 390                 395                 400 


Ala His Asn Val Val Leu Leu His Leu Leu Arg Cys Met Glu Pro Met 
                405                 410                 415     


Leu Ala Gln Asn Val Arg Gln Asn Phe Glu Leu Leu Tyr Ser Arg Arg 
            420                 425                 430         


Glu Met Leu Pro Leu Val Ser Ser His Arg Thr Arg Ile Phe Glu Ala 
        435                 440                 445             


Ile Met Ala Gly Lys Pro Glu Glu Ala Arg Glu Ala Ser His Arg His 
    450                 455                 460                 


Leu Ala Phe Ile Glu Glu Ile Leu Leu Asp Arg Ser Arg Glu Glu Ser 
465                 470                 475                 480 


Arg Arg Glu Arg Ser Leu Arg Arg Leu Glu Gln Arg Lys Asn Leu Lys 
                485                 490                 495     


Arg Ser Thr Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val 
            500                 505                 510         


Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser 
        515                 520                 525             


Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu 
    530                 535                 540                 


Lys Leu Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu 
545                 550                 555                 560 


Val Thr Thr Leu Gly Tyr Gly Leu Gln Cys Phe Ala Arg Tyr Pro Asp 
                565                 570                 575     


His Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr 
            580                 585                 590         


Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr 
        595                 600                 605             


Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu 
    610                 615                 620                 


Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys 
625                 630                 635                 640 


Leu Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys 
                645                 650                 655     


Gln Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu 
            660                 665                 670         


Asp Gly Gly Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile 
        675                 680                 685             


Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Tyr Gln 
    690                 695                 700                 


Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu 
705                 710                 715                 720 


Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu 
                725                 730                 735     


Tyr Lys 
        


<210>  5
<211>  2307
<212>  DNA
<213>  artificial

<220>
<223>  synthesized

<400>  5
atggtgagca agggcgagga gaccacaatg ggcgtaatca agcccgacat gaagatcaag       60

ctgaagatgg agggcaacgt gaatggccac gccttcgtga tcgagggcga gggcgagggc      120

aagccctacg acggcaccaa caccatcaac ctggaggtga aggagggagc ccccctgccc      180

ttctcctacg acattctgac caccgcgttc gcctacggca acagggcctt caccaagtac      240

cccgacgaca tccccaacta cttcaagcag tccttccccg agggctactc ttgggagcgc      300

accatgacct tcgaggacaa gggcatcgtg aaggtgaagt ccgacatctc catggaggag      360

gactccttca tctacgagat acacctcaag ggcgagaact tcccccccaa cggccccgtg      420

atgcagaaga agaccaccgg ctgggacgcc tccaccgaga ggatgtacgt gcgcgacggc      480

gtgctgaagg gcgacgtcaa gcacaagctg ctgctggagg gcggcggcca ccaccgcgtt      540

gacttcaaga ccatctacag ggccaagaag gcggtgaagc tgcccgacta tcactttgtg      600

gaccaccgca tcgagatcct gaaccacgac aaggactaca acaaggtgac cgtttacgag      660

agcgccgtgg cccgcaactc caccgacggc atggacgagc tgtacaagag atctggtacc      720

acaagtttgt acaaaaaagc aggctccgaa ttcgcccttg gtaccatggc ctacagcaaa      780

atccgccaac caaaactctc cgatgtgatt gagcagcaac tggagttttt gatcctcgaa      840

ggcactctcc gcccgggcga aaaactccca ccggaacgcg aactggcaaa acagtttgac      900

gtctcccgtc cctccttgcg tgaggcgatt caacgtctcg aagcgaaggg cttgttgctt      960

cgtcgccagg gtggcggcac ttttgtccag agcagcctat ggcaaagttt cagcgatccg     1020

ctggtggagc tgctctccga ccatcctgag tcacagtatg acttgctcga aacacgacac     1080

gccctggaag gtatcgccgc ttattacgcc gcgctgcgta gtaccgatga agacaaggaa     1140

cgcatccgtg aactccacca cgccatagag ctggcgcagc agtctggcga tctggacgcg     1200

gaatcaaacg ccgtactcca gtatcagatt gccgtcaccg aagcggccca caatgtggtt     1260

ctgcttcatc tgctaaggtg tatggagccg atgttggccc agaatgtccg ccagaacttc     1320

gaattgctct attcgcgtcg cgagatgctg ccgctggtga gtagtcaccg cacccgcata     1380

tttgaagcga ttatggccgg taagccggaa gaagcgcgcg aagcatcgca tcgccatctg     1440

gcctttatcg aagaaatttt gctcgacaga agtcgtgaag agagccgccg tgagcgttct     1500

ctgcgtcgtc tggagcaacg aaagaatctt aagaagggcg aattcgaccc agctttcttg     1560

tacaaagtgg tgcttaagag atctaccatg gtgagcaagg gcgaggagct gttcaccggg     1620

gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc     1680

ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagctgat ctgcaccacc     1740

ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg cctgcagtgc     1800

ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa     1860

ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc     1920

gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc     1980

aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc     2040

tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac     2100

atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac     2160

ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac     2220

cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact     2280

ctcggcatgg acgagctgta caagtaa                                         2307


<210>  6
<211>  2262
<212>  DNA
<213>  artificial

<220>
<223>  synthesized

<400>  6
atggtgagca agggcgagga gaccacaatg ggcgtaatca agcccgacat gaagatcaag       60

ctgaagatgg agggcaacgt gaatggccac gccttcgtga tcgagggcga gggcgagggc      120

aagccctacg acggcaccaa caccatcaac ctggaggtga aggagggagc ccccctgccc      180

ttctcctacg acattctgac caccgcgttc gcctacggca acagggcctt caccaagtac      240

cccgacgaca tccccaacta cttcaagcag tccttccccg agggctactc ttgggagcgc      300

accatgacct tcgaggacaa gggcatcgtg aaggtgaagt ccgacatctc catggaggag      360

gactccttca tctacgagat acacctcaag ggcgagaact tcccccccaa cggccccgtg      420

atgcagaaga agaccaccgg ctgggacgcc tccaccgaga ggatgtacgt gcgcgacggc      480

gtgctgaagg gcgacgtcaa gcacaagctg ctgctggagg gcggcggcca ccaccgcgtt      540

gacttcaaga ccatctacag ggccaagaag gcggtgaagc tgcccgacta tcactttgtg      600

gaccaccgca tcgagatcct gaaccacgac aaggactaca acaaggtgac cgtttacgag      660

agcgccgtgg cccgcaactc caccgacggc atggacgagc tgtacaagag atctggtacc      720

atggcctaca gcaaaatccg ccaaccaaaa ctctccgatg tgattgagca gcaactggag      780

tttttgatcc tcgaaggcac tctccgcccg ggcgaaaaac tcccaccgga acgcgaactg      840

gcaaaacagt ttgacgtctc ccgtccctcc ttgcgtgagg cgattcaacg tctcgaagcg      900

aagggcttgt tgcttcgtcg ccagggtggc ggcacttttg tccagagcag cctatggcaa      960

agtttcagcg atccgctggt ggagctgctc tccgaccatc ctgagtcaca gtatgacttg     1020

ctcgaaacac gacacgccct ggaaggtatc gccgcttatt acgccgcgct gcgtagtacc     1080

gatgaagaca aggaacgcat ccgtgaactc caccacgcca tagagctggc gcagcagtct     1140

ggcgatctgg acgcggaatc aaacgccgta ctccagtatc agattgccgt caccgaagcg     1200

gcccacaatg tggttctgct tcatctgcta aggtgtatgg agccgatgtt ggcccagaat     1260

gtccgccaga acttcgaatt gctctattcg cgtcgcgaga tgctgccgct ggtgagtagt     1320

caccgcaccc gcatatttga agcgattatg gccggtaagc cggaagaagc gcgcgaagca     1380

tcgcatcgcc atctggcctt tatcgaagaa attttgctcg acagaagtcg tgaagagagc     1440

cgccgtgagc gttctctgcg tcgtctggag caacgaaaga atcttaagaa gggcgaattc     1500

gacccagctt tcttgtacaa agtggtgctt aagagatcta ccatggtgag caagggcgag     1560

gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac     1620

aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag     1680

ctgatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgggc     1740

tacggcctgc agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag     1800

tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac     1860

tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg     1920

aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac     1980

aacagccaca acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc     2040

aagatccgcc acaacatcga ggacggcggc gtgcagctcg ccgaccacta ccagcagaac     2100

acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc     2160

gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc     2220

gccgccggga tcactctcgg catggacgag ctgtacaagt aa                        2262


<210>  7
<211>  2262
<212>  DNA
<213>  artificial

<220>
<223>  synthesized

<400>  7
atggtgagca agggcgagga gaccacaatg ggcgtaatca agcccgacat gaagatcaag       60

ctgaagatgg agggcaacgt gaatggccac gccttcgtga tcgagggcga gggcgagggc      120

aagccctacg acggcaccaa caccatcaac ctggaggtga aggagggagc ccccctgccc      180

ttctcctacg acattctgac caccgcgttc gcctacggca acagggcctt caccaagtac      240

cccgacgaca tccccaacta cttcaagcag tccttccccg agggctactc ttgggagcgc      300

accatgacct tcgaggacaa gggcatcgtg aaggtgaagt ccgacatctc catggaggag      360

gactccttca tctacgagat acacctcaag ggcgagaact tcccccccaa cggccccgtg      420

atgcagaaga agaccaccgg ctgggacgcc tccaccgaga ggatgtacgt gcgcgacggc      480

gtgctgaagg gcgacgtcaa gcacaagctg ctgctggagg gcggcggcca ccaccgcgtt      540

gacttcaaga ccatctacag ggccaagaag gcggtgaagc tgcccgacta tcactttgtg      600

gaccaccgca tcgagatcct gaaccacgac aaggactaca acaaggtgac cgtttacgag      660

agcgccgtgg cccgcaactc caccgacggc atggacgagc tgtacaagag atctggtacc      720

acaagtttgt acaaaaaagc aggctccgaa ttcgcccttg gtaccatggc ctacagcaaa      780

atccgccaac caaaactctc cgatgtgatt gagcagcaac tggagttttt gatcctcgaa      840

ggcactctcc gcccgggcga aaaactccca ccggaacgcg aactggcaaa acagtttgac      900

gtctcccgtc cctccttgcg tgaggcgatt caacgtctcg aagcgaaggg cttgttgctt      960

cgtcgccagg gtggcggcac ttttgtccag agcagcctat ggcaaagttt cagcgatccg     1020

ctggtggagc tgctctccga ccatcctgag tcacagtatg acttgctcga aacacgacac     1080

gccctggaag gtatcgccgc ttattacgcc gcgctgcgta gtaccgatga agacaaggaa     1140

cgcatccgtg aactccacca cgccatagag ctggcgcagc agtctggcga tctggacgcg     1200

gaatcaaacg ccgtactcca gtatcagatt gccgtcaccg aagcggccca caatgtggtt     1260

ctgcttcatc tgctaaggtg tatggagccg atgttggccc agaatgtccg ccagaacttc     1320

gaattgctct attcgcgtcg cgagatgctg ccgctggtga gtagtcaccg cacccgcata     1380

tttgaagcga ttatggccgg taagccggaa gaagcgcgcg aagcatcgca tcgccatctg     1440

gcctttatcg aagaaatttt gctcgacaga agtcgtgaag agagccgccg tgagcgttct     1500

ctgcgtcgtc tggagcaacg aaagaatctt aagagatcta ccatggtgag caagggcgag     1560

gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac     1620

aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag     1680

ctgatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgggc     1740

tacggcctgc agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag     1800

tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac     1860

tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg     1920

aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac     1980

aacagccaca acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc     2040

aagatccgcc acaacatcga ggacggcggc gtgcagctcg ccgaccacta ccagcagaac     2100

acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc     2160

gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc     2220

gccgccggga tcactctcgg catggacgag ctgtacaagt aa                        2262


<210>  8
<211>  2217
<212>  DNA
<213>  artificial

<220>
<223>  synthesized

<400>  8
atggtgagca agggcgagga gaccacaatg ggcgtaatca agcccgacat gaagatcaag       60

ctgaagatgg agggcaacgt gaatggccac gccttcgtga tcgagggcga gggcgagggc      120

aagccctacg acggcaccaa caccatcaac ctggaggtga aggagggagc ccccctgccc      180

ttctcctacg acattctgac caccgcgttc gcctacggca acagggcctt caccaagtac      240

cccgacgaca tccccaacta cttcaagcag tccttccccg agggctactc ttgggagcgc      300

accatgacct tcgaggacaa gggcatcgtg aaggtgaagt ccgacatctc catggaggag      360

gactccttca tctacgagat acacctcaag ggcgagaact tcccccccaa cggccccgtg      420

atgcagaaga agaccaccgg ctgggacgcc tccaccgaga ggatgtacgt gcgcgacggc      480

gtgctgaagg gcgacgtcaa gcacaagctg ctgctggagg gcggcggcca ccaccgcgtt      540

gacttcaaga ccatctacag ggccaagaag gcggtgaagc tgcccgacta tcactttgtg      600

gaccaccgca tcgagatcct gaaccacgac aaggactaca acaaggtgac cgtttacgag      660

agcgccgtgg cccgcaactc caccgacggc atggacgagc tgtacaagag atctggtacc      720

atggcctaca gcaaaatccg ccaaccaaaa ctctccgatg tgattgagca gcaactggag      780

tttttgatcc tcgaaggcac tctccgcccg ggcgaaaaac tcccaccgga acgcgaactg      840

gcaaaacagt ttgacgtctc ccgtccctcc ttgcgtgagg cgattcaacg tctcgaagcg      900

aagggcttgt tgcttcgtcg ccagggtggc ggcacttttg tccagagcag cctatggcaa      960

agtttcagcg atccgctggt ggagctgctc tccgaccatc ctgagtcaca gtatgacttg     1020

ctcgaaacac gacacgccct ggaaggtatc gccgcttatt acgccgcgct gcgtagtacc     1080

gatgaagaca aggaacgcat ccgtgaactc caccacgcca tagagctggc gcagcagtct     1140

ggcgatctgg acgcggaatc aaacgccgta ctccagtatc agattgccgt caccgaagcg     1200

gcccacaatg tggttctgct tcatctgcta aggtgtatgg agccgatgtt ggcccagaat     1260

gtccgccaga acttcgaatt gctctattcg cgtcgcgaga tgctgccgct ggtgagtagt     1320

caccgcaccc gcatatttga agcgattatg gccggtaagc cggaagaagc gcgcgaagca     1380

tcgcatcgcc atctggcctt tatcgaagaa attttgctcg acagaagtcg tgaagagagc     1440

cgccgtgagc gttctctgcg tcgtctggag caacgaaaga atcttaagag atctaccatg     1500

gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc     1560

gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc     1620

aagctgaccc tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc     1680

gtgaccaccc tgggctacgg cctgcagtgc ttcgcccgct accccgacca catgaagcag     1740

cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc     1800

aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg     1860

aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag     1920

ctggagtaca actacaacag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc     1980

atcaaggcca acttcaagat ccgccacaac atcgaggacg gcggcgtgca gctcgccgac     2040

cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac     2100

ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg     2160

ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaa        2217


