               SEQUENCE LISTING

<110> Ludwig-Maximilians-Universität München

<120> Method for the isolation of recombinant prion protein and the use thereof

<130> LMU140801PCT

<150> EP13182421.1
<151> 2013-08-30

<160> 5

<170> BiSSAP 1.2

<210> 1
<211> 624
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..624
<223> /mol_type="unassigned DNA"
      /note="CDS of prion protein"
      /organism="Artificial Sequence"

<400> 1
aagaagcgcc cgaagcctgg aggatggaac actgggggca gccgataccc ggggcagggc      60

agccctggag gcaaccgcta cccacctcag ggcggtggtg gctgggggca gcctcatggt     120

ggtggctggg ggcagcctca tggtggtggc tgggggcagc cccatggtgg tggctgggga     180

cagcctcatg gtggtggctg gggtcaagga ggtggcaccc acagtcagtg gaacaagccg     240

agtaagccaa aaaccaacat gaagcacatg gctggtgctg cagcagctgg ggcagtggtg     300

gggggccttg gcggctacat gctgggaagt gccatgagca ggcccatcat acatttcggc     360

agtgactatg aggaccgtta ctatcgtgaa aacatgcacc gttaccccaa ccaagtgtac     420

tacaggccca tggatgagta cagcaaccag aacaactttg tgcacgactg cgtcaatatc     480

acaatcaagc agcacacggt caccacaacc accaaggggg agaacttcac cgagaccgac     540

gttaagatga tggagcgcgt ggttgagcag atgtgtatca cccagtacga gagggaatct     600

caggcctatt accagagagg atcg                                            624


<210> 2
<211> 208
<212> PRT
<213> Artificial Sequence

<220> 
<223> recombinant prion protein

<400> 2
Lys Lys Arg Pro Lys Pro Gly Gly Trp Asn Thr Gly Gly Ser Arg Tyr 
1               5                   10                  15      
Pro Gly Gln Gly Ser Pro Gly Gly Asn Arg Tyr Pro Pro Gln Gly Gly 
            20                  25                  30          
Gly Gly Trp Gly Gln Pro His Gly Gly Gly Trp Gly Gln Pro His Gly 
        35                  40                  45              
Gly Gly Trp Gly Gln Pro His Gly Gly Gly Trp Gly Gln Pro His Gly 
    50                  55                  60                  
Gly Gly Trp Gly Gln Gly Gly Gly Thr His Ser Gln Trp Asn Lys Pro 
65                  70                  75                  80  
Ser Lys Pro Lys Thr Asn Met Lys His Met Ala Gly Ala Ala Ala Ala 
                85                  90                  95      
Gly Ala Val Val Gly Gly Leu Gly Gly Tyr Met Leu Gly Ser Ala Met 
            100                 105                 110         
Ser Arg Pro Ile Ile His Phe Gly Ser Asp Tyr Glu Asp Arg Tyr Tyr 
        115                 120                 125             
Arg Glu Asn Met His Arg Tyr Pro Asn Gln Val Tyr Tyr Arg Pro Met 
    130                 135                 140                 
Asp Glu Tyr Ser Asn Gln Asn Asn Phe Val His Asp Cys Val Asn Ile 
145                 150                 155                 160 
Thr Ile Lys Gln His Thr Val Thr Thr Thr Thr Lys Gly Glu Asn Phe 
                165                 170                 175     
Thr Glu Thr Asp Val Lys Met Met Glu Arg Val Val Glu Gln Met Cys 
            180                 185                 190         
Ile Thr Gln Tyr Glu Arg Glu Ser Gln Ala Tyr Tyr Gln Arg Gly Ser 
        195                 200                 205             



<210> 3
<211> 2415
<212> DNA
<213> Homo sapiens

<220> 
<221> source
<222> 1..2415
<223> /mol_type="unassigned DNA"
      /note="GenBank entry M13899.1"
      /organism="Homo sapiens"

<400> 3
cggcgccgcg agcttctcct ctcctcacga ccgaggcaga gcagtcatta tggcgaacct      60

tggctgctgg atgctggttc tctttgtggc cacatggagt gacctgggcc tctgcaagaa     120

gcgcccgaag cctggaggat ggaacactgg gggcagccga tacccggggc agggcagccc     180

tggaggcaac cgctacccac ctcagggcgg tggtggctgg gggcagcctc atggtggtgg     240

ctgggggcag cctcatggtg gtggctgggg gcagccccat ggtggtggct ggggacagcc     300

tcatggtggt ggctggggtc aaggaggtgg cacccacagt cagtggaaca agccgagtaa     360

gccaaaaacc aacatgaagc acatggctgg tgctgcagca gctggggcag tggtgggggg     420

ccttggcggc tacatgctgg gaagtgccat gagcaggccc atcatacatt tcggcagtga     480

ctatgaggac cgttactatc gtgaaaacat gcaccgttac cccaaccaag tgtactacag     540

gcccatggat gagtacagca accagaacaa ctttgtgcac gactgcgtca atatcacaat     600

caagcagcac acggtcacca caaccaccaa gggggagaac ttcaccgaga ccgacgttaa     660

gatgatggag cgcgtggttg agcagatgtg tatcacccag tacgagaggg aatctcaggc     720

ctattaccag agaggatcga gcatggtcct cttctcctct ccacctgtga tcctcctgat     780

ctctttcctc atcttcctga tagtgggatg aggaaggtct tcctgttttc accatctttc     840

taatcttttt ccagcttgag ggaggcggta tccacctgca gcccttttag tggtggtgtc     900

tcactctttc ttctctcttt gtcccggata ggctaatcaa tacccttggc actgatgggc     960

actggaaaac atagagtaga cctgagatgc tggtcaagcc ccctttgatt gagttcatca    1020

tgagccgttg ctaatgccag gccagtaaaa gtataacagc aaataaccat tggttaatct    1080

ggacttattt ttggacttag tgcaacaggt tgaggctaaa acaaatctca gaacagtctg    1140

aaataccttt gcctggatac ctctggctcc ttcagcagct agagctcagt atactaatgc    1200

cctatcttag tagagatttc atagctattt agagatattt tccattttaa gaaaacccga    1260

caacatttct gccaggtttg ttaggaggcc acatgatact tattcaaaaa aatcctagag    1320

attcttagct cttgggatgc aggctcagcc cgctggagca tgagctctgt gtgtaccgag    1380

aactggggtg atgttttact tttcacagta tgggctacac agcagctgtt caacaagagt    1440

aaatattgtc acaacactga acctctggct agaggacata ttcacagtga acataactgt    1500

aacatatatg aaaggcttct gggacttgaa atcaaatgtt tgggaatggt gcccttggag    1560

gcaacctccc attttagatg tttaaaggac cctatatgtg gcattccttt ctttaaacta    1620

taggtaatta aggcagctga aaagtaaatt gccttctaga cactgaaggc aaatctcctt    1680

tgtccattta cctggaaacc agaatgattt tgacatacag gagagctgca gttgtgaaag    1740

caccatcatc atagaggatg atgtaattaa aaaatggtca gtgtgcaaag aaaagaactg    1800

cttgcatttc tttatttctg tctcataatt gtcaaaaacc agaattaggt caagttcata    1860

gtttctgtaa ttggcttttg aatcaaagaa tagggagaca atctaaaaaa tatcttaggt    1920

tggagatgac agaaatatga ttgatttgaa gtggaaaaag aaattctgtt aatgttaatt    1980

aaagtaaaat tattccctga attgtttgat attgtcacct agcagatatg tattactttt    2040

ctgcaatgtt attattggct tgcactttgt gagtatctat gtaaaaatat atatgtatat    2100

aaaatatata ttgcatagga cagacttagg agttttgttt agagcagtta acatctgaag    2160

tgtctaatgc attaactttt gtaaggtact gaatacttaa tatgtgggaa acccttttgc    2220

gtggtcctta ggcttacaat gtgcactgaa tcgtttcatg taagaatcca aagtggacac    2280

cattaacagg tctttgaaat atgcatgtac tttatatttt ctatatttgt aactttgcat    2340

gttcttgttt tgttatataa aaaaattgta aatgtttaat atctgactga aattaaacga    2400

gcgaagatga gcacc                                                     2415


<210> 4
<211> 253
<212> PRT
<213> Homo sapiens

<220> 
<223> GenBank entry AAA60182.1

<400> 4
Met Ala Asn Leu Gly Cys Trp Met Leu Val Leu Phe Val Ala Thr Trp 
1               5                   10                  15      
Ser Asp Leu Gly Leu Cys Lys Lys Arg Pro Lys Pro Gly Gly Trp Asn 
            20                  25                  30          
Thr Gly Gly Ser Arg Tyr Pro Gly Gln Gly Ser Pro Gly Gly Asn Arg 
        35                  40                  45              
Tyr Pro Pro Gln Gly Gly Gly Gly Trp Gly Gln Pro His Gly Gly Gly 
    50                  55                  60                  
Trp Gly Gln Pro His Gly Gly Gly Trp Gly Gln Pro His Gly Gly Gly 
65                  70                  75                  80  
Trp Gly Gln Pro His Gly Gly Gly Trp Gly Gln Gly Gly Gly Thr His 
                85                  90                  95      
Ser Gln Trp Asn Lys Pro Ser Lys Pro Lys Thr Asn Met Lys His Met 
            100                 105                 110         
Ala Gly Ala Ala Ala Ala Gly Ala Val Val Gly Gly Leu Gly Gly Tyr 
        115                 120                 125             
Met Leu Gly Ser Ala Met Ser Arg Pro Ile Ile His Phe Gly Ser Asp 
    130                 135                 140                 
Tyr Glu Asp Arg Tyr Tyr Arg Glu Asn Met His Arg Tyr Pro Asn Gln 
145                 150                 155                 160 
Val Tyr Tyr Arg Pro Met Asp Glu Tyr Ser Asn Gln Asn Asn Phe Val 
                165                 170                 175     
His Asp Cys Val Asn Ile Thr Ile Lys Gln His Thr Val Thr Thr Thr 
            180                 185                 190         
Thr Lys Gly Glu Asn Phe Thr Glu Thr Asp Val Lys Met Met Glu Arg 
        195                 200                 205             
Val Val Glu Gln Met Cys Ile Thr Gln Tyr Glu Arg Glu Ser Gln Ala 
    210                 215                 220                 
Tyr Tyr Gln Arg Gly Ser Ser Met Val Leu Phe Ser Ser Pro Pro Val 
225                 230                 235                 240 
Ile Leu Leu Ile Ser Phe Leu Ile Phe Leu Ile Val Gly 
                245                 250             

<210> 5
<211> 8
<212> PRT
<213> Artificial Sequence

<220> 
<223> octarepeat

<400> 5
Gln Pro His Gly Gly Gly Trp Gly 
1               5               

