
                                SEQUENCE LISTING

<110> Fred Hutchinson Cancer Research Center
      Bloom, Jesse D.

<120> COMPOSITIONS AND METHODS FOR DIRECTED
  IMMUNOGEN EVOLUTION AND USES THEREOF
  

<130> 360056.419WO

<140> PCT                 
<141> 2014-08-21  

<150> US 61/870,122       
<151> 2013-08-26  

<160> 5

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 1701
<212> DNA
<213> Artificial Sequence

<220> 
<223> variant of hemagglutinin from Influenza A virus H3N2
      

<400> 1
atgaagacca tcattgcttt gagctacatt ttctgtctgg ctctcggcca agaccttcca      60
ggaaatgaca acagcacagc aacgctgtgc ctgggacatc atgcggtgcc aaacggaaca     120
ctagtgaaaa caatcacaga tgatcagatt gaagtgacta atgctactga gctagttcag     180
aactcctcaa cggggaaaat atgcaacaat cctcatcgaa tccttgatgg aataaactgc     240
acactgatag atgctctatt gggggaccct cattgtgatg tttttcaaaa tgagaaatgg     300
gaccttttcg ttgaacgcag caaagctttc agcaactgtt acccttatga tgtgccagat     360
tatgcctccc ttaggtcact agttgcctcg tcaggcactc tggagtttat caatgagagt     420
ttcaattgga ctggggtcac tcagaatggg acaagctcag cttgcaaaag gggacctaat     480
agcagttttt tcagtagact gaactggttg accaaatcag gaagcacata tccagtgctg     540
aacgtgacta tgccaaacaa tgacaatttt gacaaactat acatttgggg gattcaccac     600
ccgagcacga accaagaaca aaccagcctg tatgttcaag catcagggag agtcacagtc     660
tctaccagga gaagccagca aactataatc ccgaatatcg ggtccagacc ctgggtaagg     720
ggtctgtcta gtagaataag catctattgg acaatagtta agccgggaga cgtactggta     780
attaatagta ctgggaacct aatcgctcct cggggttatt tcaaaatgcg cactgggaaa     840
agctcaataa tgaggtcaga tgcacctatt gatacctgta tttctgaatg catcactcca     900
aatggaagca ttcccaatga caagcccttt caaaacgtaa acaagatcac atatggagca     960
tgccccaagt atgttaagca aaacaccctg aagttggcaa cagggatgcg gaatgtacca    1020
gagaaacaaa ctagaggcct attcggcgca atagcaggtt tcatagaaaa tggttgggag    1080
ggaatgatag acggttggta cggtttcagg catcaaaatt ctgagggcac aggacaagca    1140
gcagatctta aaagcactca agcagccatc gaccaaatca atgggaaatt gaacagggta    1200
atcgagaaga cgaacgagaa attccatcaa atcgaaaagg aattctcaga agtagaaggg    1260
agaattcagg acctcgagaa atacgttgaa gacactaaaa tagatctctg gtcttacaat    1320
gcggagcttc ttgtcgctct ggagaatcaa catacaattg acctgactga ctcggaaatg    1380
aacaagctgt ttgaaaaaac aaggaggcaa ctgagggaaa atgctgaaga catgggcaat    1440
ggttgcttca aaatatacca caaatgtgac aacgcttgca tagagtcaat cagaaatggg    1500
acttatgacc atgatgtata cagagacgaa gcattaaaca accggtttca gatcaaaggt    1560
gttgaactga agtctggata caaagactgg atcctgtgga tttcctttgc catatcatgc    1620
tttttgcttt gtgttgtttt gctggggttc atcatgtggg cctgccagag aggcaacatt    1680
aggtgcaaca tttgcatttg a                                              1701

<210> 2
<211> 1677
<212> DNA
<213> Artificial Sequence

<220> 
<223> variant of hemagglutinin from Influenza A virus H3N2
      

<400> 2
atgaagacca tcattgcttt gagctacatt ttctgtctgg ctctcggcca agaccttcca      60
ggaaatgaca acagcacagc aacgctgtgc ctgggacatc atgcggtgcc aaacggaaca     120
ctagtgaaaa caatcacaga tgatcagatt gaagtgacta atgctactga gctagttcag     180
agctcctcaa cggggaaaat atgcaacaat cctcatcgaa tccttgatgg aatagactgc     240
acactgatag atgctctatt gggggaccct cattgtgatg tttttcaaaa tgagacatgg     300
gaccttttcg ttgaacgcag caaagctttc agcaactgtt ttccttatga tgtgccagat     360
tatgcctccc ttaggtcact agttgcctcg tcaggcactc tggagtttat cactgagggt     420
ttcacttgga ctggggtcac tcagaatggg ggaagcaatg cttgcaaaag gggacctggt     480
agcggttttt tcagtagact gaactggttg accaaatcag gaagcacata tccagtgctg     540
aacgtgacta tgccaaacaa tgacaatttt gacaaactat acatttgggg gattttccac     600
ccgagcacga accaagagca aaccagcgcg tatgttcaag catcagggag agtcacagtc     660
tctaccagga gaagccagca aactataatc ccgaatatcg ggtccagaag aataagcatc     720
tattggacaa tagttaagcc gggagacgta ctggtaatta atagtaatgg gaacctaatc     780
gctcctcggg gttatttcaa aatgcgcact gggaaaagct caataatgag gtcagatgca     840
cctattgata cctgtatttc tgaatgcatc actccaaatg gaagcattcc caatgacaag     900
ccctttcaaa acgtaaacaa gatcacatat ggagcatgcc ccaagtatgt taagcaaaac     960
accctgaagt tggcaacagg gatgcggaat gtaccagaga aacaaactag aggcctattc    1020
ggcgcaatag caggtttcat agaaaatggt tgggagggaa tgatagacgg ttggtacggt    1080
ttcaggcatc aaaattctga gggcacagga caagcagcag atcttaaaag cactcaagca    1140
gccatcgacc aaatcaatgg gaaattgaac agggtaatcg agaagacgaa cgagaaattc    1200
catcaaatcg aaaaggaatt ctcagaagta gaagggagaa ttcaggacct cgagaaatac    1260
gttgaagaca ctaaaataga tctctggtct tacaatgcgg agcttcttgt cgctctggag    1320
aatcaacata caattgacct gactgactcg gaaatgaaca agctgtttga aaaaacaagg    1380
aggcaactga gggaaaatgc tgaagacatg ggcaatggtt gcttcaaaat ataccacaaa    1440
tgtgacaacg cttgcataga gtcaatcaga aatgggactt atgaccatga tgtatacaga    1500
gacgaagcat taaacaaccg gtttcagatc aaaggtgttg aactgaagtc tggatacaaa    1560
gactggatcc tgtggatttc ctttgccata tcatgctttt tgctttgtgt tgttttgctg    1620
gggttcatca tgtgggcctg ccagagaggc aacattaggt gcaacatttg catttga       1677

<210> 3
<211> 1677
<212> DNA
<213> Artificial Sequence

<220> 
<223> variant of hemagglutinin from Influenza A virus H3N2
      

<400> 3
atgaagacca tcattgcttt gagctacatt ttctgtctgg ctctcggcca agaccttcca      60
ggaaatgaca acagcacagc aacgctgtgc ctgggacatc atgcggtgcc aaacggaaca     120
ctagtgaaaa caatcacaga tgatcagatt gaagtgacta atgctactga gctagttcag     180
aactcctcaa cggggaaaat atgcaacaat cctcatcgaa tccttgatgg aataaactgc     240
acactgatag atgctctatt gggggaccct cattgtgatg tttttcaaaa tgagaaatgg     300
gaccttttcg ttgaacgcag caaagctttc agcaactgtt ttccttatga tgtgccagat     360
tatgcctccc ttaggtcact agttgcctcg tcaggcactc tggagtttat caatgagagt     420
ttcaattgga ctggggtcac tcagaatggg acaagctcag cttgcaaaag gggacctaat     480
agcagttttt tcagtagact gaactggttg accaaatcag gaagcacata tccagtgctg     540
aacgtgacta tgccaaacaa tgacaatttt gacaaactat acatttgggg gattttccac     600
ccgagcacga accaagagca aaccagcgcg tatgttcaag catcagggag agtcacagtc     660
tctaccagga gaagccagca aactataatc ccgaatatcg ggtccagaag aataagcatc     720
tattggacaa tagttaagcc gggagacgta ctggtaatta atagtactgg gaacctaatc     780
gctcctcggg gttatttcaa aatgcgcact gggaaaagct caataatgag gtcagatgca     840
cctattgata cctgtatttc tgaatgcatc actccaaatg gaagcattcc caatgacaag     900
ccctttcaaa acgtaaacaa gatcacatat ggagcatgcc ccaagtatgt taagcaaaac     960
accctgaagt tggcaacagg gatgcggaat gtaccagaga aacaaactag agaactattc    1020
ggcgcaatag caggtttcat agaaaatggt tgggagggaa tgatagacgg ttggtacggt    1080
ttcaggcatc aaaattctga gggcacagga caagcagcag atcttaaaag cactcaagca    1140
gccatcgacc aaatcaatgg gaaattgaac agggtaatcg agaagacgaa cgaggaattc    1200
catcaaatcg aaaaggaatt ctcagaagta gaagggagaa ttcaggacct cgagaaatac    1260
gttgaagaca ctaaaataga tctctggtct tacaatgcgg agcttcttgt cgctctggag    1320
aatcaacata caattgacct gactgactcg gaaatgaaca agctgtttga aaaaacaagg    1380
aggcaactga gggaaaatgc tgaagacatg ggcaatggtt gcttcaaaat ataccacaaa    1440
tgtgacaacg cttgcataga gtcaatcaga aatgggactt atgaccatga tgtatacaga    1500
gacgaagcat taaacaaccg gtttcagatc aaaggtgttg aactgaagtc tggatacaaa    1560
gactggatcc tgtggatttc ctttgccata tcatgctttt tgctttgtgt tgttttgctg    1620
gggttcatca tgtgggcctg ccagagaggc aacattaggt gcaacatttg catttga       1677

<210> 4
<211> 1362
<212> DNA
<213> Artificial Sequence

<220> 
<223> variant of neuraminidase from Influenza A virus H3N2
      

<400> 4
atgaatccaa accagaaaat aataaccatt gggtcaatct gtatggtagt cggaataatt      60
agcctaatat tgcaaatagg aaatataatc tcaatatgga ttagccattc aattcaaacc     120
ggaaatcaaa accatactgg aatatgcaac caaggcagca ttacctataa agttgttgct     180
gggcaggact caacttcagt gatattaacc ggcaattcat ctctttgtcc catccgtggg     240
tgggctatac acagcaaaga caatggcata agaattggtt ccaaaggaga cgtttttgtc     300
ataagagagc cttttatttc atgttctcac ttggaatgca ggaccttttt tctgactcaa     360
ggcgccttac tgaatgacaa gcattcaagg gggaccttta aggacagaag cccttatagg     420
gccttaatga gctgccctgt cggtgaagct ccgtccccgt acaattcaag gtttgaatcg     480
gttgcttggt cagcaagtgc atgtcatgat ggaatgggct ggctaacaat cggaatttct     540
ggtccagatg atggagcagt ggctgtatta aaatacaacc gcataataac tgaaaccata     600
aaaagttgga ggaagaatat attgagaaca caagagtctg aatgtacctg tgtaaatggt     660
tcatgtttta ccataatgac cgatggccca agtgatgggc tggcctcgta caaaattttc     720
aagatcgaga aggggaaggt tactaaatcg atagagttga atgcacctaa ttctcactac     780
gaggaatgtt cctgttaccc tgataccggc aaagtgatgt gtgtgtgcag agacaattgg     840
cacggttcga accgaccatg ggtgtccttc gaccaaaacc tagattataa aataggatac     900
atctgcagtg gggttttcgg tgacaacccg cgtcccaaag atggaacagg cagctgtggc     960
ccagtgtctg ctgatggagc aaacggagta aagggatttt catataagta tggcaatggt    1020
gtttggatag gaaggactaa aagtgacagt tccagacatg ggtttgagat gatttgggat    1080
cctaatggat ggacagagac tgatagtagg ttctctatga gacaagatgt tgtggcaata    1140
actaatcggt cagggtacag cggaagtttc gttcaacatc ctgagctaac agggctagac    1200
tgtatgaggc cttgcttctg ggttgaatta atcagggggc tacctgagga ggacgcaatc    1260
tggactagtg ggagcatcat ttctttttgt ggtgtgaata gtgatactgt agattggtct    1320
tggccagacg gtgctgagtt gccgttcacc attgacaagt ag                       1362

<210> 5
<211> 1362
<212> DNA
<213> Artificial Sequence

<220> 
<223> variant of neuraminidase from Influenza A virus H3N2
      

<400> 5
atgaatccaa accagaaaat aataaccatt gggtcaatct gtatggtagt cggaataatt      60
agcctaatat tgcaaatagg aaatataatc tcaatatgga ttagccattc aattcaaacc     120
ggaaatcaaa accatactgg aatatgcaac caaggcagca ttacctataa agttgttgct     180
gggcaggact caacttcagt gatattaacc ggcaattcat ctctttgtcc catccgtggg     240
tgggctatac acagcaaaga caatggcata agaattggtt ccaaaggaga cgtttttgtc     300
ataagagagc cttttatttc atgttctcac ttggaatgca ggaccttttt tctgactcaa     360
ggcgccttac tgaatgacaa gcattcaagg aggaccttta aggacagaag cccttatagg     420
gccttaatga gctgccctgt cggtgaagct ccgtccccgt acaattcaag gtttgaatcg     480
gttgcttggt cagcaagtgc atgtcatgat ggaatgggct ggctaacaat cggaatttct     540
ggtccagatg atggagcagt ggctgtatta aaatacaacc gcataataac tgaaaccata     600
aaaagttgga ggaagaatat attgagaaca caagagtctg aatgtacctg tgtaaatggt     660
tcatgtttta ccataatgac cgatggccca agtgatgggc tggcctcgta caaaattttc     720
aagatcgaga aggggaaggt tactaaatcg atagagttga atgcacctaa ttctcactac     780
gaggaatgtt cctgttaccc tgataccggc aaagtgatgt gtgtgtgcag agacaattgg     840
cacggttcga accgaccatg ggtgtccttc gaccaaaacc tagattataa aataggatac     900
atctgcagtg gggttttcgg tgacaacccg cgtcccaaag atggaacagg cagctgtggc     960
ccagtgtctg ctgatggagc aaacggagta aagggatttt catataagta tggcaatggt    1020
gtttggatag gaaggactaa aagtgacagt tccagacatg ggtttgagat gatttgggat    1080
cctaatggat ggacagagac tgatagtagg ttctctatga gacaagatgt tgtggcaata    1140
actaatcggt cagggtacag cggaagtttc gttcaacatc ctgagctaac agggctagac    1200
tgtatgaggc cttgcttctg ggttgaatta atcagggggc tacctgagga ggacgcaatc    1260
tggactagtg ggagcatcat ttctttttgt ggtgtgaata gtgatactgt agattggtct    1320
tggccagacg gtgctgagtt gccgttcacc attgacaagt ag                       1362
